CN212504881U - Convenient microorganism sampling culture apparatus and culture system thereof - Google Patents
Convenient microorganism sampling culture apparatus and culture system thereof Download PDFInfo
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- CN212504881U CN212504881U CN202021448568.7U CN202021448568U CN212504881U CN 212504881 U CN212504881 U CN 212504881U CN 202021448568 U CN202021448568 U CN 202021448568U CN 212504881 U CN212504881 U CN 212504881U
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Abstract
The utility model relates to a convenient microorganism sample culture apparatus and cultivation system thereof, convenient microorganism sample culture apparatus include box body, lid, backup pad and agar culture medium, the backup pad pass the lid, together fixed with the lid, the backup pad on be provided with the agar culture medium, the box body install together with the lid, form the cultivation space, the volume in cultivation space be greater than the volume of agar culture medium. The utility model discloses a convenient microorganism sample culture apparatus easy operation, effect that test disinfecting and sterilizing equipment that can convenient and fast is favorable to disinfecting and sterilizing equipment's using widely.
Description
Technical Field
The utility model relates to an environmental surface microorganism sample culture apparatus specifically is a convenient microorganism sample culture apparatus and culture system thereof.
Background
The sterilization and disinfection equipment is a common equipment in life and production, and the sterilization and disinfection effect of the sterilization and disinfection equipment is an important parameter of the equipment. The existing experimental process for measuring the sterilization and disinfection effects of the medicines is complex. The sampling culture method of the microorganisms on the surface of the object comprises the following steps: (1) using cotton swab soaked with sterilized normal saline to pick up 25cm on the surface of the object to be detected2The area of the cotton swab is smeared in the cotton swab for 10 times, then a part of the cotton swab contacted with the hand is cut off, and the cotton swab is put into a sampling tube containing 10mL of sterilized normal saline for inspection. (2) The test tube with the cotton swab was shaken thoroughly. The liquid is 10-1And (4) diluting the solution. If the pollution is serious, the dilution can be increased by ten times, and 1ml10 is sucked-1Adding the sample solution into 9ml of sterile water, and mixing uniformly, wherein the solution is 10-2Diluting the solution by analogy to 10-3、10-4. (3) Selecting 1-2 dilutions and respectively taking 1ml of sample liquid to respectively inject into an aseptic plate by aseptic operation, preparing two plates (parallel samples) for each dilution, pouring the plate counting agar culture medium which is melted and cooled to about 45 ℃ into the plates, and fully mixing about 15ml of the plate counting agar culture medium. (4) After the agar is solidified, the plate is turned over, and the plate is placed at 36 +/-1 ℃ for culturing for 48h and then counted. (5) Detecting and culturing fungi, namely selecting 3-4 dilutions, dropwise adding 1mL of suspension into each dish (the diameter is 9cm), pouring the dissolved and to-be-solidified Bengal culture medium, uniformly mixing, and culturing in an incubator at 25 ℃ for 5-7 days. This method has the following problems: (1) operating environment demanding, other steps than samplingThe procedure needs to be performed in a sterile environment, requiring specialized equipment. (2) The operation requirement is high, the operation is required to be carried out by operators in a sterile environment, and the requirement on the operators is strict. Because of the above reasons, the rapid and convenient microorganism detection can not be carried out on objects or in sealed spaces, but the sterilization and disinfection effect can not be conveniently and rapidly displayed by the existing disinfection equipment, thereby influencing the popularization and the use of the sterilization and disinfection equipment.
SUMMERY OF THE UTILITY MODEL
The utility model relates to a convenient microorganism sampling culture apparatus, cultivation system and sample cultivation method thereof has solved the biological sample detection of current environment thing and has operated complicacy, the problem that the operational environment required height.
In order to solve the technical problem, the utility model discloses a technical scheme does:
the utility model provides a convenient microorganism sample culture apparatus, includes box body, lid, backup pad and agar-agar culture medium, the backup pad pass the lid, together fixed with the lid, the backup pad on be provided with the agar-agar culture medium, the box body install together with the lid, form the culture space, the volume in culture space be greater than the volume of agar-agar culture medium.
Preferably: the box cover is provided with a plurality of bosses.
Preferably: the box cover is a microorganism breathable sealing film, gauze, a plastic film or kraft paper.
Preferably: the supporting plate is provided with a fixing and enhancing mechanism for increasing the fixing of the agar culture medium and the supporting plate.
Preferably: the fixed reinforcing mechanism is a fixing hole, a fixing sheet and a fixing line which are arranged on the supporting plate.
The utility model also provides a convenient microorganism sample culture system, including convenient microorganism sample culture apparatus and incubator, the incubator include box and case lid, box and case lid install together, the box in be provided with a plurality of fixed convenient microorganism sample culture apparatus's fixed establishment.
Preferably: the fixing mechanism is a plurality of fixing grooves arranged at the lower part of the box body.
Preferably: the fixing groove comprises a lower small groove and an upper large groove.
Preferably: the convenient microorganism sampling culture system further comprises a temperature control mechanism and a humidity control mechanism.
The utility model has the advantages that:
the utility model discloses a fix the microorganism agar-agar culture medium of sterilization in the backup pad, put into the box body, make box body and the sealed aseptic save of lid, during the use, detach sealed package, take out the backup pad fast, the agar-agar culture medium above using cleans contact object surface sampling area, accomplishes the sample operation, then puts back the box body, covers the sealed cultivation of lid, and the box body is put back in quick sample, can guarantee the interior sterile environment of box, can realize the aseptic cultivation of microorganism. The utility model discloses directly with the culture medium with treat the regional direct contact of sample, accomplish the sample, dilute and the coating step, the step is simple, convenient operation, can convenient and fast efficient treat that detection object or environment carry out the microorganism sample and train the count, the disinfection effect of calculation disinfecting equipment that can convenient and fast is favorable to disinfection and sterilization equipment's using widely.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of the convenient microorganism sampling and culturing device of the present invention;
FIG. 2 is another schematic structural view of the convenient microorganism sampling and culturing device of the present invention;
FIG. 3 is a schematic view of another structure of the convenient microorganism sampling and culturing device of the present invention;
fig. 4 is a schematic structural view of the box cover and the supporting plate of the present invention;
fig. 5 is another schematic structural view of the cover and the support plate of the present invention;
fig. 6 is another schematic structural view of the cover and the support plate of the present invention;
FIG. 7 is a schematic front view of the convenient microorganism sampling and culturing system of the present invention;
FIG. 8 is a schematic left side view of the portable microorganism sampling and culturing system of the present invention;
FIG. 9 is another schematic front view of the portable microorganism sampling and culturing system of the present invention;
in the figure, 1 is a box cover, 2 is a boss, 3 is a support plate, 4 is a fixing hole, 5 is a box body, 6 is an agar culture medium, 7 is gauze, 8 is a support body, 9 is a fixing sheet, 10 is a fixing line, 11 is a box cover, 12 is a box body, 13 is a fixing groove, and 14 is a temperature control mechanism.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Example 1
As shown in figures 1-6, a convenient microorganism sampling culture device comprises a box body 5, a box cover 1, a support plate 3 and an agar culture medium 6, wherein the support plate 3 penetrates through the box cover 1 and is fixed with the box cover 1, the agar culture medium 6 is arranged on the support plate 3, the box body 5 and the box cover 1 are installed together to form a culture space, and the volume of the culture space is larger than that of the agar culture medium 6.
The box body 5 can be set into different shapes according to actual requirements, such as rectangle, circle, round platform shape and the like. In this embodiment, a rectangle is used. The box body 5 can be used for culturing different bacteria types according to needs, and different box bodies 5 are arranged. As shown in fig. 1 and 2, fig. 1 shows a large cartridge 5, and fig. 2 shows a small cartridge 5.
The box cover 1 is provided with a plurality of bosses 2, and the bosses 2 are matched with the box body 5 to seal the box body 5. One, two or more bosses 2 may be provided. As shown in fig. 1, two bosses 2 are arranged in fig. 1 and are gradually reduced. The large boss 2 is sealed with the large box body 5, and the small box body 5 is sealed with the small boss 2. As shown in fig. 3 and 5, a boss 2 is provided.
The box cover 1 can be an integrally formed plastic box cover 1, and can also be made of other materials. As shown in FIG. 3, for culturing microorganisms requiring a large amount of oxygen, the box cover 1 may be made of a gas permeable material such as a microorganism sealing film, gauze 7, plastic film or kraft paper, and a support 8 for supporting and fixing the gas permeable material is further provided. As shown in fig. 1, the present embodiment uses a case 5 integrally formed of plastic.
As shown in FIGS. 1, 5 and 6, the supporting plate 3 is provided with a fixation enhancing mechanism for increasing the fixation of the agar medium 6 to the supporting plate 3. The fixing reinforcing mechanism is a fixing hole 4 (shown in figure 1), a fixing sheet 9 (shown in figure 5) and a fixing line 10 (shown in figure 6) which are arranged on the supporting plate 3. The fixing hole 4 is used in this embodiment.
Example 2
As shown in figures 7-9, a convenient microorganism sampling culture system, including convenient microorganism sampling culture apparatus and incubator, convenient microorganism sampling culture apparatus in this implementation includes a convenient microorganism sampling culture apparatus, including box body 5, lid 1, backup pad 3 and agar medium 6, backup pad 3 pass lid 1, together fixed with lid 1, backup pad 3 on be provided with agar medium 6, box body 5 and lid 1 install together, form the culture space, the volume of culture space be greater than the volume of agar medium 6.
The box body 5 is rectangular, circular, truncated cone-shaped and the like.
The box cover 1 is provided with 2 bosses 2, and the bosses 2 are matched with the box body 5 to seal the box body 5. The box cover 1 is an integrally formed plastic box cover 1. The supporting plate 3 is provided with a fixing and enhancing mechanism for increasing the fixing of the agar culture medium 6 and the supporting plate 3. The fixing reinforcing mechanism is a fixing hole 4 provided on the support plate 3.
The incubator comprises an incubator body 12 and an incubator cover 11, wherein the incubator body 12 and the incubator cover 11 are installed together, and a plurality of fixing mechanisms for fixing the convenient microorganism sampling and culturing device are arranged in the incubator body 12.
In this embodiment, two rows of 5 fixing mechanisms are provided.
The fixing mechanism is a plurality of fixing grooves 13 arranged at the lower part of the box body 12.
The fixing groove 13 of the present embodiment includes a lower small groove and an upper large groove. Also can choose to set up fixed recess 13 alone, the utility model discloses can realize that the convenient microorganism sample culture apparatus of two kinds of different box body 5 sizes cultivates the operation.
As shown in FIG. 9, the convenient microorganism sampling and culturing system further comprises a temperature control mechanism 14. The temperature control mechanism 14 generally employs an electric heating device and a thermometer, which facilitates accurate determination of the culture environment and facilitates the microorganisms to be in a proper growth environment.
Example 3
A convenient microorganism sampling and culturing method adopts the convenient microorganism sampling and culturing system of the embodiment 2, and comprises the following steps:
(1) after the convenient microorganism sampling culture system is prepared by aseptic operation, the convenient microorganism sampling culture system is put into a box body for sealing and aseptic storage;
(2) when sampling detection is needed, the supporting plate is taken out, the agar culture medium is enabled to wipe the sampling area on the surface of the object to be detected or to be placed in the space to be detected for a plurality of times, then the agar culture medium is rapidly placed into the box body, and the box cover and the box body are installed together;
(3) inserting the convenient microorganism sampling culture device after sampling into the fixed groove, covering the box cover, and then culturing;
(4) after the completion of the culture, the microorganism count and species confirmation were performed. In the operation of step 2 of this embodiment, an alcohol lamp may be used near the sampling point to create a local sterile environment, so as to improve the sampling accuracy.
For sampling of microorganisms in the air, the agar medium 6 is placed in the air to be sampled for a certain period of time, and then placed in the box 5.
To the disinfecting equipment that disinfects, through the utility model discloses a device convenient and fast's sample is cultivateed the microorganism on air or object surface around the disinfection of disinfecting, can convenient and fast survey out disinfecting equipment that disinfects. Compared with the existing method for measuring the sterilization and disinfection effects, the method has the advantages of simple steps, no need of dilution, coating and other steps, and can effectively improve the efficiency and reduce the pollution probability. No aseptic equipment is needed to provide an aseptic environment, the operation is simple and convenient, and the cost is low.
For sampling objects with different microorganism contents, convenient microorganism sampling culture devices with different sizes can be adopted to realize the dilution of the microorganisms, and the agar culture medium 6 has a proper amount of bacterial colonies.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The utility model provides a convenient microorganism sampling culture apparatus which characterized in that: including box body, lid, backup pad and agar-agar culture medium, the backup pad pass the lid, together fixed with the lid, the backup pad on be provided with the agar-agar culture medium, the box body install together with the lid, form the culture space, the volume in culture space be greater than the volume of agar-agar culture medium.
2. The convenient microorganism sampling and culturing device according to claim 1, wherein: the box cover is provided with a plurality of bosses.
3. The convenient microorganism sampling and culturing device according to claim 1, wherein: the box cover is a microorganism breathable sealing film, gauze, a plastic film or kraft paper.
4. The convenient microorganism sampling and culturing device according to claim 1, wherein: the supporting plate is provided with a fixing and enhancing mechanism for increasing the fixing of the agar culture medium and the supporting plate.
5. The convenient microorganism sampling and culturing device according to claim 4, wherein: the fixed reinforcing mechanism is a fixing hole, a fixing sheet and a fixing line which are arranged on the supporting plate.
6. A convenient microorganism sampling culture system which is characterized in that: the convenient microorganism sampling and culturing device comprises the convenient microorganism sampling and culturing device and an incubator according to any one of claims 1 to 5, wherein the incubator comprises a box body and a box cover, the box body and the box cover are installed together, and a plurality of fixing mechanisms for fixing the convenient microorganism sampling and culturing device are arranged in the box body.
7. The convenient microorganism sampling and culturing system according to claim 6, wherein: the fixing mechanism is a plurality of fixing grooves arranged at the lower part of the box body.
8. The convenient microorganism sampling and culturing system according to claim 7, wherein: the fixing groove comprises a lower small groove and an upper large groove.
9. A convenient microorganism sampling and culturing system according to any one of claims 6 to 8, wherein: the convenient microorganism sampling culture system further comprises a temperature control mechanism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021448568.7U CN212504881U (en) | 2020-07-21 | 2020-07-21 | Convenient microorganism sampling culture apparatus and culture system thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021448568.7U CN212504881U (en) | 2020-07-21 | 2020-07-21 | Convenient microorganism sampling culture apparatus and culture system thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212504881U true CN212504881U (en) | 2021-02-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021448568.7U Active CN212504881U (en) | 2020-07-21 | 2020-07-21 | Convenient microorganism sampling culture apparatus and culture system thereof |
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| Country | Link |
|---|---|
| CN (1) | CN212504881U (en) |
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- 2020-07-21 CN CN202021448568.7U patent/CN212504881U/en active Active
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Address after: 472400 Caogou village, Hongyang Town, Mianchi County, Sanmenxia City, Henan Province Patentee after: Henan maianda biosafety Technology Co.,Ltd. Address before: 472400 Caogou village, Hongyang Town, Mianchi County, Sanmenxia City, Henan Province Patentee before: DIANCHI MAIANDA AGRICULTURAL TECHNOLOGY CO.,LTD. |
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| CP01 | Change in the name or title of a patent holder |