CN212504822U

CN212504822U - Fluorescent quantitative PCR instrument

Info

Publication number: CN212504822U
Application number: CN202020929524.XU
Authority: CN
Inventors: 曹婧; 陈礼朋; 任亚楠; 张向兵; 赵胜杰
Original assignee: Henan Geyue Testing Technology Co ltd
Current assignee: Henan Geyue Testing Technology Co ltd
Priority date: 2020-05-27
Filing date: 2020-05-27
Publication date: 2021-02-09
Anticipated expiration: 2030-05-27

Abstract

The utility model discloses a fluorescence quantitative PCR instrument, which comprises a body, wherein an internal hollow box body is arranged at the lower side of the body, an exhaust pipe and an air inlet pipe which are respectively communicated with the inside of the body and the box body are arranged at the bottom of the body, a first fan which blows air into the exhaust pipe from the inside of the body is arranged at one end of the exhaust pipe communicated with the inside of the body, a cooling assembly arranged inside the box body is connected between the exhaust pipe and the air inlet pipe, and an auxiliary heat dissipation device is arranged in the box body; the utility model discloses simple structure dispels the heat including circulative cooling's mode in the body, has avoided this internal air and outside air contact, and the dust in the outside air can not enter into the body, has guaranteed the normal work of internal circuit, has mentioned the life of PCR appearance.

Description

Fluorescent quantitative PCR instrument

Technical Field

The utility model belongs to the technical field of fluorescence detection, a fluorescence quantitative PCR appearance is related to.

Background

The fluorescent quantitative PCR is a new quantitative experimental technology which is introduced by American application Biosystems company in 1996, and is characterized in that a PCR product is marked and tracked through a fluorescent dye or a fluorescent marked specific probe, the reaction process is monitored on line in real time, the product is analyzed by combining corresponding software, and the initial concentration of a sample template to be detected is calculated.

The PCR appearance that uses at present can produce higher temperature at the during operation, consequently can set up the louvre on the PCR appearance body generally and dispel the heat, prevent that the too high problem of burning out the internal circuit of internal temperature, install radiator fan in the PCR appearance that has, convection current through the air is to PCR appearance inside fan heat, can inhale the PCR appearance with the dust in the air through louvre and radiator fan, thereby influence the work of internal circuit, reduce the life of PCR appearance.

SUMMERY OF THE UTILITY MODEL

To the above problem, the utility model provides a fluorescence quantitative PCR appearance, thereby fine solution prior art dust carry out the inside problem that influences the work of internal circuit of PCR appearance from the louvre.

In order to realize the purpose, the utility model discloses a technical scheme as follows: a fluorescent quantitative PCR instrument comprises a body, wherein a box body with a hollow inner part is arranged at the lower side of the body, an exhaust pipe and an air inlet pipe which are respectively communicated with the inside of the body and the box body are arranged at the bottom of the body, a first fan for blowing air into the exhaust pipe from the inside of the body is arranged at one end of the exhaust pipe communicated with the inside of the body, a cooling assembly arranged in the box body is connected between the exhaust pipe and the air inlet pipe, and an auxiliary heat dissipation device is arranged in the box body;

the cooling assembly comprises a plurality of circulation pipelines and semiconductor refrigeration pieces, wherein one ends of the circulation pipelines are communicated with the exhaust pipe, the semiconductor refrigeration pieces are arranged on the side faces of the circulation pipelines, the other ends of the circulation pipelines are communicated with the air inlet pipe, and the refrigeration faces of the semiconductor refrigeration pieces are attached to the side faces of the circulation pipelines along the length direction of the circulation pipelines.

Furthermore, supplementary heat abstractor is including setting up in the box side and towards the air intake of circulating line one end, set up in air intake department from the box outside to the inside second fan of blowing of box and set up in the box side and towards the air exit of the circulating line other end.

Furthermore, a first metal filter screen is arranged between the second fan and the circulating pipeline, and a second metal filter screen fixed on the side wall of the box body is arranged at the air outlet.

Furthermore, the filtering precision of the first metal filter screen and the second metal filter screen is 200-300 meshes/inch.

Furthermore, a plurality of circulation pipelines are equidistantly arranged in parallel and are integrally connected, and the two ends of each circulation pipeline are respectively provided with a connecting part which is respectively communicated with the exhaust pipe and the air inlet pipe in a sealing way.

Furthermore, the cross section of each circulation pipeline is rectangular, and the semiconductor refrigeration pieces are arranged on two opposite sides of each circulation pipeline.

Compared with the prior art, the utility model discloses following beneficial effect has:

1. in the utility model, the first fan sucks the heat generated by the circuit work in the body into the exhaust pipe, the heat enters the circulating pipeline from the exhaust pipe, the semiconductor refrigeration piece arranged on the circulating pipeline reduces the temperature in the circulating pipeline to cool the hot air entering the circulating pipeline, and the cold air in the circulating pipeline enters the body from the air inlet pipe to cool the circuit work; the utility model discloses simple structure dispels the heat including circulative cooling's mode in the body, has avoided this internal air and outside air contact, and the dust in the outside air can not enter into the body, has guaranteed the normal work of internal circuit, has improved the life of PCR appearance.

2. The auxiliary heat dissipation device can dissipate heat generated by the semiconductor refrigeration piece to the outside of the box body from the box body, and the cooling effect of the circulating pipeline is guaranteed.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a sectional view taken along line A-A of FIG. 1;

fig. 3 is a perspective view of a flow conduit.

In the figure: the device comprises a body 1, a box body 2, an exhaust pipe 3, an air inlet pipe 4, a first fan 5, a circulating pipeline 6, a semiconductor refrigerating sheet 7, an air inlet 8, a second fan 9, an air outlet 10, a first metal filter screen 11, a second metal filter screen 12, a connecting part 13, a mounting plate 14 and a mounting hole 15.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1, the fluorescence quantitative PCR instrument of the present invention comprises a body 1, wherein a working circuit concentrated on a PCB board is installed in the body 1, an internally hollow box 2 is disposed at the lower side of the body 1, an exhaust pipe 3 and an air inlet pipe 4 which are respectively communicated with the inside of the body 1 and the box 2 are disposed at the bottom of the body 1, a first fan 5 which blows air into the exhaust pipe 3 from the inside of the body 1 is disposed at one end of the exhaust pipe 3 communicated with the inside of the body 1, a cooling assembly disposed inside the box 2 is connected between the exhaust pipe 3 and the air inlet pipe 4, and an auxiliary heat dissipation device is disposed in the box 2;

the cooling assembly comprises a plurality of circulation pipelines 6 and semiconductor refrigerating sheets 7, wherein one ends of the circulation pipelines are communicated with the exhaust pipe 3, the semiconductor refrigerating sheets 7 are arranged on the side faces of the circulation pipelines 6, the other ends of the circulation pipelines 6 are communicated with the air inlet pipe 4, and refrigerating faces of the semiconductor refrigerating sheets 7 are attached to the side faces of the circulation pipelines 6 along the length direction of the circulation pipelines 6.

In the embodiment, through holes with the same cross section shapes as the end parts of the exhaust pipe 3 and the air inlet pipe 4 are respectively formed in the bottom surface of the body 1 and the top surface of the box body 2, the end parts of the air inlet pipe 4 and the exhaust pipe 3 are respectively inserted into the through holes and are connected with the box body 2 and the body 1 in a full-length welding mode so as to ensure the sealing property of the connection part; the first fan 5 is fixed to the bottom of the body 1 by means of screws; the refrigeration surface of the semiconductor refrigeration piece 7 is bonded on the circulation pipeline 6 through epoxy resin, the type of the semiconductor refrigeration piece 7 is TEC1-12706, a lead of the semiconductor refrigeration piece 7 is electrically connected with a working circuit in the body 1, holes are formed in one sides of the box body 2 and the body 1, the lead of the semiconductor refrigeration piece 7 penetrates out of the holes in the box body 2, penetrates into the holes in the body 1 and is connected with the working circuit, sealant is filled in the holes, the first fan 5 is also electrically connected with the working circuit in the body 1, and when the working circuit works, the semiconductor refrigeration piece 7 and the first fan 5 also work.

Specifically, as shown in fig. 1, the auxiliary heat dissipation device includes an air inlet 8 disposed at a side surface of the box 2 and facing one end of the circulation duct 6, a second fan 9 disposed at the air inlet 8 for blowing air from outside the box 2 to inside the box 2, and an air outlet 10 disposed at a side surface of the box 2 and facing the other end of the circulation duct 6; the refrigerating surface of the semiconductor refrigerating sheet 7, which is attached to the circulating pipeline 6, refrigerates to enable the circulating pipeline 6 to have a lower temperature, meanwhile, the heating surface of the semiconductor refrigerating sheet 7 radiates heat into the box body 2, and the second fan 9 discharges the heat out of the box body 2 from the air outlet 10, so that the continuous refrigerating performance of the semiconductor refrigerating sheet 7 is improved, and the normal work of a cooling assembly is guaranteed; the second fan 9 is also electrically connected with the working circuit in the body 1 and works simultaneously with the first fan 5.

Preferably, a first metal filter 11 is arranged between the second fan 9 and the circulating pipeline 6, and a second metal filter 12 fixed on the side wall of the box body 2 is arranged at the air outlet 10; the shape of the cross section of the first metal filter screen 11 is the same as that of the cross section of the box body 2, the second metal filter screen 12 is fixed on the box body 2 through screws, and moreover, the box body 2 is formed by splicing six sheet metal parts through screws, so that the flow pipeline 6, the second fan 9, the first metal filter screen 11 and the second metal filter screen 12 are convenient to install; first metal filters 11 and second metal filters 12 all can effectually prevent that the dust in the box 2 outside air from entering into box 2, avoid the dust to adhere to circulation pipeline 6 and semiconductor refrigeration piece 7 on, guaranteed the refrigeration effect of semiconductor refrigeration piece 7.

Preferably, the filtering precision of the first metal filter 11 and the second metal filter 12 is 200-300 meshes/inch, which effectively filters dust in the air entering the box 2.

In this embodiment, as shown in fig. 3, a plurality of the circulation pipes 6 are arranged in parallel at equal intervals and integrally connected, and both ends of the circulation pipes 6 are provided with connecting portions 13 respectively communicated with the exhaust duct 3 and the air inlet duct 4 in a sealing manner; specifically, the connecting part 13 is a pipeline with a rectangular cross section, the lower end of the connecting part 13 is integrally formed with the circulating pipelines 6 and communicated with the plurality of circulating pipelines 6, the edge of the upper end of the connecting part 13 is provided with a mounting plate 14, the mounting plate 14 is provided with a mounting hole 15, the mounting plate 14 is also provided with a sealing gasket, the mounting plate 14 is fixed on the box body 2 through bolts and is communicated with the exhaust pipe 3 and the air inlet pipe 4; a plurality of flow ducts 6 can improve the cooling efficiency.

In this embodiment, as shown in fig. 2, every the shape of the cross section of circulation pipeline 6 is the rectangle, semiconductor refrigeration piece 7 sets up in every circulation pipeline 6 relative both sides, can further improve circulation pipeline 6's cooling efficiency for cooling module cooling effect is more obvious, has improved the utility model discloses a reliability.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.

Claims

1. A fluorescence quantitative PCR instrument is characterized in that: the air cooling device comprises a body, wherein a box body with a hollow interior is arranged on the lower side of the body, an exhaust pipe and an air inlet pipe which are respectively communicated with the interior of the body and the interior of the box body are arranged at the bottom of the body, a first fan for blowing air into the exhaust pipe from the interior of the body is arranged at one end of the exhaust pipe communicated with the interior of the body, a cooling assembly arranged in the box body is connected between the exhaust pipe and the air inlet pipe, and an auxiliary heat dissipation device is arranged in the box body;

2. The fluorescence quantitative PCR instrument according to claim 1, characterized in that: the auxiliary heat dissipation device comprises an air inlet, a second fan and an air outlet, wherein the air inlet is formed in the side face of the box body and faces one end of the circulating pipeline, the second fan is arranged at the air inlet and blows air from the outside of the box body to the inside of the box body, and the air outlet is formed in the side face of the box body and faces the other end of the circulating pipeline.

3. The fluorescence quantitative PCR instrument according to claim 2, characterized in that: a first metal filter screen is arranged between the second fan and the circulating pipeline, and a second metal filter screen fixed on the side wall of the box body is arranged at the air outlet.

4. The fluorescence quantitative PCR instrument according to claim 3, wherein: the filtering precision of the first metal filter screen and the second metal filter screen is 200-300 meshes/inch.

5. The fluorescence quantitative PCR instrument according to claim 1, characterized in that: a plurality of the circulation pipeline is equidistantly arranged in parallel and is integrally connected, and the two ends of the circulation pipeline are respectively provided with a connecting part which is hermetically communicated with the exhaust pipe and the air inlet pipe.

6. The fluorescence quantitative PCR instrument according to claim 1, characterized in that: the cross section of each circulation pipeline is rectangular, and the semiconductor refrigeration pieces are arranged on two opposite sides of each circulation pipeline.