CN212451432U - Sheath liquid circulating water bath temperature control device of flow cytometry sorting instrument - Google Patents
Sheath liquid circulating water bath temperature control device of flow cytometry sorting instrument Download PDFInfo
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- CN212451432U CN212451432U CN202020971441.7U CN202020971441U CN212451432U CN 212451432 U CN212451432 U CN 212451432U CN 202020971441 U CN202020971441 U CN 202020971441U CN 212451432 U CN212451432 U CN 212451432U
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Abstract
The utility model provides a flow cytometer sheath liquid circulation water bath temperature control device. Specifically, the apparatus comprises: the water bath barrel comprises a barrel body, the barrel body is provided with an inner wall and an outer wall, the inner wall and the outer wall form a closed hollow interlayer together, and the barrel body is also provided with a first water inlet and a first water outlet which are communicated with the hollow interlayer; the circulating water supply device includes: a water temperature adjusting system and a circulating water pumping system. Circulating water with preset water temperature circularly flows between the interlayer of the water bath barrel and the circulating water supply device through the water pump and the pipeline, so that the constant temperature of the sheath fluid in the water bath barrel is ensured. The utility model discloses a device can make sheath liquid temperature invariant, and easily operation, can make flow cytometer experimental result more reliable and more stable.
Description
Technical Field
The utility model relates to a solution control by temperature change technical field, concretely relates to flow cytometer sheath liquid circulation water bath temperature control device.
Background
Flow cytometry is a technique for individually, multiparameter, rapid qualitative, quantitative analysis or sorting of single lines of cells or biological particles, etc. in a rapid linear flow state. The single cell suspension with fluorescence to be detected enters a flow chamber under the pressure of gas, cells are arranged in a single row and sequentially pass through a detection area of the flow chamber under the coating of sheath liquid, laser vertically irradiates a sample flow of the detection area, and the sample generates scattered light and laser fluorescence under the irradiation of the sample. The scattered light is divided into forward scattered light (reflecting the size of the cell volume) and side scattered light (reflecting the complexity of the intracellular particles), and the fluorescent signal represents information inside and outside the labeled cell. Then, these optical signals are converted into electric signals, which are transmitted to a computer and are simulated into image data files by corresponding software. The cells to be sorted are separated into single cells wrapped by sheath fluid through high-frequency oscillation, the target cells are charged with positive electricity or negative electricity and are deflected under the action of a high-voltage electric field to fall into corresponding collectors, and therefore the cells are separated.
Factors influencing the cell sorting effect are many, including preparation of earlier single cell suspension, setting of sorting parameters, collection mode of sorted cells and the like. At present, in order to ensure the activity and the state of cells, a general flow cytometry sorting instrument has the temperature control functions of a sample loading bin and a sorting bin, but has no effective temperature control system for sheath fluid wrapping the cells. The sheath fluid temperature is related to the room temperature, and as the startup time increases, the sheath fluid temperature increases, which affects the stability of the fluid flow and thus the sorting effect.
Therefore, the circulating water bath temperature control device of the sheath fluid of the flow cytometer, which can make the temperature of the sheath fluid constant and is easy to operate, is urgently needed in the field.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a can make sheath liquid constancy of temperature, flow cytometer sheath liquid circulation water bath temperature control device of easily operating.
The utility model provides a flow cytometer sheath liquid circulation water bath temperature control device, the device includes:
the water bath barrel comprises a barrel body, and the barrel body is a hollow container with an opening at the upper part; the barrel body is provided with an inner wall and an outer wall, the inner wall and the outer wall form a closed hollow interlayer together, the barrel body is also provided with a first water inlet and a first water outlet which are communicated with the hollow interlayer, and the first water inlet is positioned below the first water outlet;
a circulating water providing apparatus, comprising: a water temperature regulating system and a circulating water pumping system;
the water temperature adjusting system includes: the device comprises a heat exchange container, a heater and/or a refrigerator, a temperature sensor and a temperature controller;
the temperature sensor is arranged to measure the temperature of water in the heat exchange container; the heater and/or the refrigerator are/is arranged to adjust the water temperature in the heat exchange container; the temperature controller comprises a temperature display and a temperature input key; the temperature controller is used for displaying the real-time temperature of the temperature sensor, inputting a preset temperature and controlling the start and the stop of the heater and/or the refrigerator;
the heat exchange container is provided with a second water inlet and a second water outlet;
the circulating water supply device comprises a third water inlet and a third water outlet;
the second water inlet is connected with the third water inlet, and the second water outlet is connected with the third water outlet; and
the circulating water pumping system comprises a water pump, a water inlet pipe and a water outlet pipe; the water inlet pipe is detachably connected with the third water outlet and the first water inlet; the water outlet pipe is detachably connected with the third water inlet and the first water outlet; the water pump is arranged to circulate water.
In another preferred example, the heat exchange container is a coiled pipe, a rectangular water tank or a cylindrical water tank.
In another preferred example, the temperature sensor is used for measuring the temperature of the second water outlet.
In another preferred example, a drain valve connected with the hollow interlayer is arranged below the barrel body.
In another preferred embodiment, the device further comprises a sealing cover plate, wherein the sealing cover plate comprises a circular ring part and a first sealing ring, the first sealing ring is arranged on the inner edge of the circular ring part, and the sealing cover plate is detachably arranged on the upper edge of the barrel body.
In another preferred example, a second sealing ring is arranged on the upper edge of the barrel body, and the second sealing ring provides sealing for the joint of the barrel body and the sealing cover plate.
In another preferred example, the ring member is a segmented structure including two semicircles, and the first seal ring is a segmented seal ring matched with the segmented structure.
In another preferred example, the barrel body forms a cylindrical space with an upper opening inside, and the diameter of the cylindrical space is 250-250.5mm, and the height of the cylindrical space is 280-290 mm.
In another preferred example, the first water inlet and the first water outlet are respectively located on the same vertical section of the barrel body.
In another preferred example, 2-4 supporting legs are arranged below the water bath barrel.
In another preferred example, a sliding wheel is arranged below the supporting leg, and the sliding wheel is provided with a locking device.
In another preferred embodiment, the outer wall is provided with a handle.
It is understood that within the scope of the present invention, the above-mentioned technical features of the present invention and those specifically described below (e.g. in the examples) can be combined with each other to constitute new or preferred technical solutions. Not to be reiterated herein, but to the extent of space.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the apparatus of the present invention;
FIG. 2 is a top view of an embodiment of the water bath bucket of the present invention;
FIG. 3 is a cross-sectional view of an embodiment of the water bath bucket of the present invention;
in the drawings, each reference numeral denotes:
the water-bath-type solar water heater comprises a temperature control system 1, a water bath barrel 2, a first water outlet 3, a first water inlet 4, a liquid outlet pipe 5, a liquid inlet pipe 6, a third water outlet 7, a third water inlet 8, a sealing cover plate 9, supporting legs 10, universal wheels 11, a drain valve 12, a handle 13 and a hollow interlayer 14.
Detailed Description
The inventor provides a sheath fluid circulating water bath temperature control device of a flow cytometer through extensive and intensive research and a large number of screening and tests. The utility model discloses a device is including placing sheath liquid bucket and having the interbedded water bath bucket of cavity to and make water have the circulating water of predetermined temperature and provide the device, the device can make the sheath liquid in the sheath liquid bucket remain constant temperature throughout, thereby improves flow cytometry sorter's stability. The utility model discloses has accomplished on this basis.
Term(s) for
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
As used herein, the term "comprising" or "includes" can be open, semi-closed, and closed. In other words, the term also includes "consisting essentially of …," or "consisting of ….
As used herein, the term "flow cytometer sheath fluid circulating water bath temperature control device" and "the device of the present invention" are used interchangeably and refer to the flow cytometer sheath fluid circulating water bath temperature control device of the present invention.
In the description of the present invention, it is to be understood that the terms "coaxial", "bottom", "one end", "top", "middle", "other end", "upper", "one side", "top", "inner", "front", "center", "both ends", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.
Furthermore, the terms "first", "second", "third", "fourth" are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated, whereby the features defined as "first", "second", "third", "fourth" may explicitly or implicitly include at least one such feature.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "disposed," "connected," "fixed," "screwed" and the like are to be construed broadly, e.g., as meaning fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.
Sheath liquid circulating water bath temperature control device of flow cytometry
The utility model provides a flow cytometer sheath liquid circulation water bath temperature control device, the device includes:
the water bath barrel comprises a barrel body, and the barrel body is a hollow container with an opening at the upper part; the barrel body is provided with an inner wall and an outer wall, the inner wall and the outer wall form a closed hollow interlayer together, the barrel body is also provided with a first water inlet and a first water outlet which are communicated with the hollow interlayer, and the first water inlet is positioned below the first water outlet;
a circulating water providing apparatus, comprising: a water temperature regulating system and a circulating water pumping system;
the water temperature adjusting system includes: the device comprises a heat exchange container, a heater and/or a refrigerator, a temperature sensor and a temperature controller;
the temperature sensor is arranged to measure the temperature of water in the heat exchange container; the heater and/or the refrigerator are/is arranged to adjust the water temperature in the heat exchange container; the temperature controller comprises a temperature display and a temperature input key; the temperature controller is used for displaying the real-time temperature of the temperature sensor, inputting a preset temperature and controlling the start and the stop of the heater and/or the refrigerator;
the heat exchange container is provided with a second water inlet and a second water outlet;
the circulating water supply device comprises a third water inlet and a third water outlet;
the second water inlet is connected with the third water inlet, and the second water outlet is connected with the third water outlet; and
the circulating water pumping system comprises a water pump, a water inlet pipe and a water outlet pipe; the water inlet pipe is detachably connected with the third water outlet and the first water inlet; the water outlet pipe is detachably connected with the third water inlet and the first water outlet; the water pump is arranged to circulate water.
In another preferred example, the heat exchange container is a coiled pipe, a rectangular water tank or a cylindrical water tank.
In another preferred example, the temperature sensor is used for measuring the temperature of the second water outlet.
In another preferred example, a drain valve connected with the hollow interlayer is arranged below the barrel body.
In another preferred embodiment, the device further comprises a sealing cover plate, wherein the sealing cover plate comprises a circular ring part and a first sealing ring, the first sealing ring is arranged on the inner edge of the circular ring part, and the sealing cover plate is detachably arranged on the upper edge of the barrel body.
In another preferred example, a second sealing ring is arranged on the upper edge of the barrel body, and the second sealing ring provides sealing for the joint of the barrel body and the sealing cover plate.
In another preferred example, the ring member is a segmented structure including two semicircles, and the first seal ring is a segmented seal ring matched with the segmented structure.
In another preferred example, the barrel body forms a cylindrical space with an upper opening inside, and the diameter of the cylindrical space is 250-250.5mm, and the height of the cylindrical space is 280-290 mm.
In another preferred example, the first water inlet and the first water outlet are respectively located on the same vertical section of the barrel body.
In another preferred example, 2-4 supporting legs are arranged below the water bath barrel.
In another preferred example, a sliding wheel is arranged below the supporting leg, and the sliding wheel is provided with a locking device.
In another preferred embodiment, the outer wall is provided with a handle.
The utility model discloses a main advantage includes:
1. the device of the utility model has simple structure and convenient operation, can effectively control the temperature of the sheath liquid and ensure the stability of the liquid flow when the flow cell sorter is used;
2. the device of the utility model can make the circulating water flow circularly, is self-integrated, and is suitable for being used in a clean laboratory without a water tap and a drain pipe;
3. the size of the sheath liquid circulating water bath barrel in the device of the utility model is designed to be suitable for BD Aria I, BD Aria II and BD Aria III series flow cytometry sorters, and the universality is wide;
4. the utility model discloses a device is simultaneously because of having installed the universal wheel that has the locking function, and convenient device's removal is with fixed, need not artifical transport, labour saving and time saving.
The present invention will be further described with reference to specific embodiments. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The experimental procedures, in which specific conditions are not noted in the following examples, are generally carried out under conventional conditions or conditions recommended by the manufacturers. Unless otherwise indicated, percentages and parts are by weight.
Example 1
This embodiment provides a basis the utility model discloses a flow cytometer sheath liquid circulation water bath temperature control device. As shown in fig. 1-3.
The device comprises a water bath barrel 2 and a circulating water supply device 1.
The water bath barrel 2 comprises a barrel body which is a hollow container with an opening at the upper part; the barrel body has inner wall and outer wall, inner wall and outer wall form a confined cavity intermediate layer together, the barrel body still have with the first water inlet and the first delivery port of cavity intermediate layer intercommunication, just first water inlet is located first delivery port below.
In this embodiment, the device further has a sealing cover 9, wherein the sealing cover has a circular ring part and a first sealing ring (e.g. a silicone ring), wherein the first sealing ring is disposed on an inner edge of the circular ring part, and the sealing cover is detachably disposed on an upper edge of the barrel body. The inner diameter of the sealing cover plate is matched with the outer diameter of the sheath liquid barrel. Can provide support and better sealed sheath liquid bucket and ladle body inner space's clearance for the sheath liquid bucket.
In another preferred example, the sealing cover plate is of a two-semicircular segmented structure. Because the sheath liquid barrel itself has a plurality of pipelines connected with it, the segmented design can make the sealing cover plate install and remove from the side of the sheath liquid barrel. Preferably, the first sealing ring is correspondingly segmented.
The sections of the sealing cover plate can be connected through a common mode in the field, for example, a connecting piece and a screw are used for connecting, a screw hole for respectively connecting the two adjacent sections is formed in the connecting piece, a corresponding screw hole is formed in one end of the sectional cover plate, and two ends of the connecting piece are respectively fixed on different sections through the screw to be connected.
The existence of the sealing cover plate can ensure that the size of the opening of the water bath barrel is easy to control without adjusting the size of the barrel body.
Preferably, a second sealing ring is arranged on the upper edge of the barrel body. The second sealing washer parcel along a week on the ladle body, be located between sealed apron and the ladle body upper limb, can for sealed apron with the connection on the ladle body upper limb provides sealedly. The annular part of the sealing cover 9 has a width of 5-10cm, may provide an operating plane, and may provide attachment points for the placement of the first sealing ring. The existence of sealing washer can make the temperature in the sheath liquid bucket receive ambient temperature to influence littleer, provides the buffering for the contact of sheath liquid bucket and water bath bucket simultaneously.
Preferably, a handle is further arranged on the sealing cover plate. The handle can make the sealing cover plate easy to remove.
Preferably, the first water inlet 4 and the first water outlet 3 are respectively positioned on the same vertical section of the barrel body. To maximize the distance between the two.
The circulating water supply device 1 includes: a water temperature adjusting system and a circulating water pumping system.
The water temperature adjusting system includes: the device comprises a heat exchange container, a heater and a refrigerator, a temperature sensor and a temperature controller.
The temperature sensor is arranged to measure the temperature of water in the heat exchange container.
The heater and the refrigerator are arranged to regulate the temperature of water in the heat exchange container.
The temperature controller comprises a temperature display and a temperature input key; the temperature controller is used for displaying the real-time temperature of the temperature sensor, inputting a preset temperature and controlling the start and the stop of the heater and the refrigerator. The temperature controller may use a temperature controller commonly used in the art, such as WK-SM 3A.
The temperature controller preferably controls the temperature in a range suitable for survival of the cells, such as 10-37 ℃.
The water temperature regulating system can also be a water bath kettle commonly used in a laboratory.
The heat exchange container is provided with a second water inlet and a second water outlet; the circulating water supply device comprises a third water inlet and a third water outlet; the second water inlet is connected with the third water inlet, and the second water outlet is connected with the third water outlet. Preferably, the temperature sensor is used for measuring the temperature of the second water outlet.
In another preferred example, the water inlet of the heat exchange container is the water inlet of the circulating water supply device. The water outlet of the heat exchange container is the water outlet of the circulating water supply device. Preferably, the heat exchange container is also provided with a separate water filling port, so that water can be conveniently introduced into the circulating pipeline.
When the heat exchange container is an open container, such as a water bath, the water inlet is the water outlet.
The circulating water pumping system comprises a water pump, a water inlet pipe 6 and a water outlet pipe 5; the water inlet pipe 6 is detachably connected with the third water outlet 7 and the first water inlet 4; the water outlet pipe 5 is detachably connected with the third water inlet 8 and the first water outlet 3.
Through the pipeline intercommunication, heat exchange container, inlet tube, cavity intermediate layer and outlet pipe form a closed loop pipeline, but the circulating water circulation flow.
The water pump is arranged to circulate water. The water pump may be connected to the circulation line in a manner common in the art. Preferably, the water pump is disposed downstream of the heat exchange vessel. The water pump may be a pump commonly used in the art. Such as a peristaltic pump or the like. For example a peristaltic pump may be provided to cooperate with the inlet pipe 6 (hose) to convey the circulating water.
Preferably, the heat exchange container, the heater, the refrigerator, the temperature sensor, the temperature controller and/or the water pump are integrated in a cabinet. Preferably, a sliding wheel is arranged below the chassis.
Preferably, the outer wall of the tub is provided with 2 handles 13.
The lower part of the water bath barrel is provided with a supporting leg 10. Below the legs there are sliding wheels 11, preferably universal wheels. The sliding wheel is provided with a locking device, and after the position is determined, the device can be fixed and cannot easily slide.
Preferably, the legs 10 are angled at an angle consistent with the angle of tilt of the flow cytometer carriage. Thereby guaranteeing that the sheath liquid pressure is not influenced after the sheath liquid circulating water bath temperature control device of the flow cytometry sorting instrument is loaded.
Preferably, the heat exchange container is a coiled pipe, a rectangular water tank or a cylindrical water tank.
Preferably, a drain valve 12 (such as a ball valve) connected with the hollow interlayer 14 is arranged below the barrel body.
Preferably, a sliding wheel is arranged below the supporting leg. The sliding wheel has a locking device.
More preferably, the water bath barrel 2 forms a cylindrical space with an upper opening in the barrel body. Meanwhile, the inner diameter is 250mm, and the inner height is 280 mm. This size is designed to fit the sheath fluid bucket of the BD Aria I, BD Aria II, BD Aria III series flow cytometric sorter. The external diameter of the barrel body is 292mm, and the height of the barrel body is 300 mm. The integral height of the barrel body and the supporting legs is 620 mm. A first water outlet 3 is arranged at one side of the outer wall of the bucket body and is 50mm away from the top of the bucket, and a water inlet 4 is arranged at the other side of the outer wall of the bucket body and is 30mm away from the bottom of the bucket. The drain valve 12 is level with the first water inlet 4 or below the first water inlet 4. The first water outlet 3 and the first water inlet 4 adopt CPP quick connectors. The barrel body, the supporting legs, the handle and the cover plate are all made of SS304 steel materials.
All documents mentioned in this application are incorporated by reference into this application as if each were individually incorporated by reference. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the above teachings of the present invention, and these equivalents also fall within the scope of the appended claims.
Claims (10)
1. A flow cytometer sheath fluid circulation water bath temperature control device is characterized in that the device comprises:
the water bath barrel comprises a barrel body, and the barrel body is a hollow container with an opening at the upper part; the barrel body is provided with an inner wall and an outer wall, the inner wall and the outer wall form a closed hollow interlayer together, the barrel body is also provided with a first water inlet and a first water outlet which are communicated with the hollow interlayer, and the first water inlet is positioned below the first water outlet;
a circulating water providing apparatus, comprising: a water temperature regulating system and a circulating water pumping system;
the water temperature adjusting system includes: the device comprises a heat exchange container, a heater and/or a refrigerator, a temperature sensor and a temperature controller;
the temperature sensor is arranged to measure the temperature of water in the heat exchange container; the heater and/or the refrigerator are/is arranged to adjust the water temperature in the heat exchange container; the temperature controller comprises a temperature display and a temperature input key; the temperature controller is used for displaying the real-time temperature of the temperature sensor, inputting a preset temperature and controlling the start and the stop of the heater and/or the refrigerator;
the heat exchange container is provided with a second water inlet and a second water outlet;
the circulating water supply device comprises a third water inlet and a third water outlet;
the second water inlet is connected with the third water inlet, and the second water outlet is connected with the third water outlet; and
the circulating water pumping system comprises a water pump, a water inlet pipe and a water outlet pipe; the water inlet pipe is detachably connected with the third water outlet and the first water inlet; the water outlet pipe is detachably connected with the third water inlet and the first water outlet; the water pump is arranged to circulate water.
2. The flow cytometer sheath fluid circulating water bath temperature control device of claim 1, wherein the heat exchange container is a coiled tube, a rectangular water tank or a cylindrical water tank.
3. The flow cytometer sheath fluid circulating water bath temperature control device of claim 1, wherein the temperature sensor is configured to measure the temperature of the second water outlet.
4. The flow cytometer sheath fluid circulating water bath temperature control device of claim 1, wherein a drain valve connected with the hollow interlayer is arranged below the barrel body.
5. The sheath fluid circulating water bath temperature control device of the flow cytometer as described in claim 1, further comprising a sealing cover plate, wherein said sealing cover plate has a circular ring component and a first sealing ring, said first sealing ring is disposed on the inner edge of said circular ring component, and said sealing cover plate is detachably disposed on the upper edge of the bucket body.
6. The circulating water bath temperature control device for sheath fluid of a flow cytometer as described in claim 5, wherein a second sealing ring is disposed on the upper edge of said barrel body, and said second sealing ring provides a seal for the junction of said barrel body and said sealing cover plate.
7. The flow cytometer sheath fluid circulating water bath temperature control device of claim 5, wherein the ring component is a segmented structure comprising two semicircles, and the first sealing ring is a segmented sealing ring matching with the segmented structure.
8. The sheath fluid circulating water bath temperature control device of claim 1, wherein a cylindrical space with an upper opening is formed inside the barrel body, and the diameter of the cylindrical space is 250-250.5mm, and the height of the cylindrical space is 280-290 mm.
9. The circulating water bath temperature control device for sheath fluid of a flow cytometer as described in claim 1, wherein 2-4 legs are disposed below said water bath barrel.
10. The flow cytometer sheath fluid circulating water bath temperature control device of claim 9, wherein a sliding wheel is disposed below the support leg, and the sliding wheel has a locking device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020971441.7U CN212451432U (en) | 2020-06-01 | 2020-06-01 | Sheath liquid circulating water bath temperature control device of flow cytometry sorting instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020971441.7U CN212451432U (en) | 2020-06-01 | 2020-06-01 | Sheath liquid circulating water bath temperature control device of flow cytometry sorting instrument |
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| CN212451432U true CN212451432U (en) | 2021-02-02 |
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| CN202020971441.7U Active CN212451432U (en) | 2020-06-01 | 2020-06-01 | Sheath liquid circulating water bath temperature control device of flow cytometry sorting instrument |
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| CN (1) | CN212451432U (en) |
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