CN218345451U - Liquid filling system for cell culture workstation and cell culture workstation - Google Patents

Abstract

The utility model relates to a cell culture technical field specifically provides a liquid filling system and cell culture workstation for cell culture workstation, and the liquid filling system who aims at solving current cell culture workstation often appears prefilling inadequately, leads to the volume of the functional liquid of filling to deviate to influence the problem of the cultivation effect of cell. Therefore, the utility model discloses a liquid filling system includes storage device, liquid supply pipe, liquid feeding pipeline, pump unit, first detection component and controlling means, and liquid supply pipe's one end and storage device intercommunication, the other end and pump unit's entrance point intercommunication, liquid feeding pipe's one end and pump unit's exit end intercommunication, first detection component can detect the liquid that flows from liquid supply pipe's the other end, and controlling means can control the action of pump unit according to the testing result that detects the component. Through such setting, can guarantee that the filling pipeline is full of functional liquid for the volume of the functional liquid of filling is more accurate.

Description

Liquid filling system for cell culture workstation and cell culture workstation

Technical Field

The utility model relates to a cell culture technical field specifically provides a liquid filling system and cell culture workstation for cell culture workstation.

Background

With the rapid development of medical and aesthetic medicine, life and health and other industries, cell medicine is playing an increasingly important role, and people have higher and higher requirements on cells with high quality, uniformity and stable sources.

Traditional cells are obtained by artificial culture, and a plurality of workers are required to wear protective articles such as cleaning clothes, masks and the like and enter a clean room for cell culture after thorough disinfection. However, dust and bacteria can be brought into a laboratory in the manual operation process, potential pollution risks exist, too much labor force is occupied, the working efficiency is low, and the labor intensity and dangerousness of personnel can be increased due to the sterile environment of the laboratory, toxic medicines and sharp equipment.

In recent years, a cell culture workstation capable of realizing automated cell culture has come to be provided with a sealed incubator and various automated devices installed in the incubator, and the devices are matched with each other under the control of a control system, so that the automated cell culture can be well realized.

In the process of cell culture, a plurality of functional liquids, such as culture solution, digestive juice, and washing solution, are required to be used, and when the functional liquids are to be filled, the functional liquids need to be filled in a predetermined volume. However, the liquid filling systems of the existing cell culture workstations often have insufficient pre-filling, which causes the volume of the functional liquid to be filled to be deviated, thereby affecting the culture effect of the cells.

Therefore, there is a need in the art for a new solution to the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the above technical problem, promptly, the liquid filling system of solving current cell culture workstation often appears prefilling the insufficient condition, leads to the volume of the functional liquid of filling to appear the deviation to influence the problem of the cultivation effect of cell.

In a first aspect, the utility model provides a liquid filling system for cell culture workstation, liquid filling system includes storage device, liquid supply pipe way, liquid feeding pipeline, pump device, first detection component and controlling means, wherein, storage device is used for the required functional liquid of storage cell culture, liquid supply pipe way one end with storage device intercommunication, liquid supply pipe way the other end with the entrance point intercommunication of pump device, liquid feeding pipeline's one end with the exit end intercommunication of pump device, pump device with first detection component all with the controlling means communication is connected, first detection component can detect the follow the liquid that the other end of liquid feeding pipeline flows out, controlling means can according to the testing result control of detection component the pump device action.

In the above preferred technical solution of the liquid filling system for the cell culture workstation, the liquid filling system further includes a second detection member disposed in the liquid adding pipe, and the second detection member is in communication connection with the control device and can detect bubbles in the liquid adding pipe.

In the above preferred technical solution of the liquid filling system for a cell culture workstation, the liquid filling system further includes a third detection member disposed on the storage device, and the third detection member is communicatively connected to the control device and is capable of detecting the weight of the functional liquid located in the storage device.

In the above preferred technical solution of the liquid filling system for the cell culture workstation, the liquid filling system further includes a refrigerating device and a heating device, the refrigerating device is disposed on the storage device and is capable of cooling the functional liquid in the storage device, and the heating device is disposed on the liquid supply pipeline and is capable of heating the functional liquid in the liquid supply pipeline.

In a preferred technical solution of the above liquid filling system for a cell culture workstation, the heating device includes a heating box and a heating member mounted on the heating box, a part of the liquid supply pipeline is located in the heating box, the heating box stores therein a heating liquid, and the heating member is capable of heating the heating liquid so as to heat the functional liquid in the liquid supply pipeline through the heating liquid.

In the above preferred embodiment of the liquid filling system for the cell culture workstation, the liquid supply pipe located in the heating chamber extends in a spiral configuration.

In a preferred embodiment of the above liquid filling system for a cell culture workstation, the liquid filling system further includes a temperature sensor installed in the heating tank, and the temperature sensor is configured to detect a temperature of the heated liquid.

In the above preferable technical scheme of the liquid filling system for the cell culture workstation, the heating box is provided with a heat-insulating layer.

In the above preferred embodiment of the liquid filling system for a cell culture workstation, the storage means is mounted outside the incubator of the cell culture workstation.

In a second aspect, the present invention also provides a cell culture workstation comprising the above-described liquid filling system.

Under the condition that adopts above-mentioned technical scheme, the utility model discloses a liquid filling system includes storage device, liquid supply pipe way, liquid feeding pipeline, pump unit, first detection component and controlling means, wherein, storage device is used for the required functional liquid of storage cell culture, liquid supply pipe way's one end with storage device intercommunication, liquid supply pipe way's the other end with pump unit's entrance point intercommunication, liquid feeding pipe way's one end with pump unit's exit end intercommunication, pump unit with first detection component all with the controlling means communication is connected, first detection component can detect the follow the liquid that the other end of liquid feeding pipeline flows out, controlling means can according to the testing result control of detection component the pump unit action. Through such setting, can guarantee that the filling pipeline is full of functional liquid, improve the sufficiency of prefilling for the volume of the functional liquid of filling is more accurate, improves the cultivation effect of cell.

Further, the utility model discloses a liquid filling system is still including setting up second in the liquid feeding pipeline detects the component, the second detect the component with controlling means communication connection just can detect and be located the bubble in the liquid feeding pipeline. Through such setting, can avoid being located to have the bubble in the functional liquid of liquid feeding pipeline, further improve the accuracy of the functional liquid's of filling volume.

Still further, the utility model discloses a liquid filling system still includes refrigerating plant and heating device, refrigerating plant sets up storage device is last and can be right functional liquid in the storage device cools down, heating device sets up on the liquid supply pipe way and can be right functional liquid in the liquid supply pipe way heats. By means of the arrangement, the functional liquid can be stored in the storage device for low-temperature storage, the storage time of the functional liquid is prolonged, and when the functional liquid is required to be used, the functional liquid is heated to a required temperature by the heating device.

Still further, the utility model discloses a heating device includes the heating cabinet and installs heating member on the heating cabinet, supply liquid pipeline's partly to be located in the heating cabinet, the heating cabinet is the storage has the heating fluid body, heating member can be right the heating fluid body heats with passing through the heating fluid body is right supply liquid pipeline is interior functional liquid heats.

Through such setting for heating temperature is more stable, and the heating effect is better.

Still further, the liquid supply pipe located in the heating box extends in a spiral structure. Through the arrangement, the contact area between the liquid supply pipeline and the heating liquid can be increased, and the heating of the functional liquid in the liquid supply pipeline is facilitated.

Still further, the utility model discloses a liquid filling system is still including setting up temperature sensor in the heating cabinet, temperature sensor is used for detecting the temperature of the heating liquid. Through such an arrangement, it is convenient to detect the temperature of the heating liquid in the heating tank so as to accurately control the temperature of the functional liquid.

Still further, an insulating layer is arranged on the heating box. By such an arrangement, it is more advantageous to keep the temperature of the heated liquid in the heating tank stable.

Still further, the utility model discloses install storage device in the outside of the incubator of cell culture workstation. Through such setting, when supplementing the functional liquid in the storage device, need not to open the incubator to can avoid causing the pollution to the environment in the incubator, in addition, still need not to occupy the inner space of incubator.

Drawings

Preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which:

FIG. 1 is a schematic diagram of a cell culture workstation according to the present invention;

list of reference numbers:

1. an incubator; 2. a pump device; 3. a storage device; 4. a liquid supply conduit; 5. a heating box; 6. a heating member; 7. a liquid feeding pipe; 8. a first detection member; 9. a third detecting member.

Detailed Description

Preferred embodiments of the present invention will be described below with reference to the accompanying drawings. It should be understood by those skilled in the art that these embodiments are only for explaining the technical principle of the present invention, and are not intended to limit the scope of the present invention.

It should be noted that in the description of the present invention, the terms "inside", "outside", "upper", "lower", etc. indicating directions or positional relationships are based on the directions or positional relationships shown in the drawings, which are only for convenience of description, and do not indicate or imply that the devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.

Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "disposed," "connected," and "mounted" are to be construed broadly and may include, for example, a fixed connection, a detachable connection, or an integral connection. The specific meaning of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

The liquid filling system based on the prior cell culture workstation pointed out by the background art often has the problem of insufficient pre-filling, which causes the deviation of the volume of the filled functional liquid, thereby influencing the culture effect of cells. The utility model provides a liquid filling system for cell culture workstation aims at detecting the first component that detects of the liquid that flows from the export of liquid feeding pipeline through the setting to cooperation controlling means controls pump unit, can guarantee that filling pipeline intussuseption is full of functional liquid, improves the sufficiency of prefilling, makes the volume of the functional liquid of filling more accurate, improves the cultivation effect of cell.

Specifically, as shown in fig. 1, the cell culture workstation of the present invention comprises an incubator 1, a pump device 2, a storage device 3, a liquid supply pipeline 4, a liquid supply pipeline 7, a first detection component 8 and a control device (not shown in the figure), wherein the pump device 2 and the first detection component 8 are all in communication connection with the control device.

The storage device 3 is used for storing functional liquids required for cell culture, such as a culture solution, a digestion solution, and a washing solution.

With continued reference to fig. 1, one end of the liquid supply pipe 4 communicates with the storage device 3, the other end of the liquid supply pipe 4 communicates with the inlet end of the pump device 2, the outlet end of the pump device 2 communicates with one end of the liquid charging pipe 7, the pump device 2 is installed in the incubator 1, and the first detection member 8 can detect the liquid flowing out from the other end of the liquid charging pipe 7.

In the process of cell culture, when functional liquid needs to be filled into a cell culture bottle, the pump device 2 is started, the functional liquid stored in the storage device 3 flows towards the pump device 2 along the liquid supply pipeline 4 under the action of the pump device 2, and the functional liquid enters the liquid filling pipeline 7 after flowing through the pump device 2.

The first detection member 8 detects the other end of the liquid feeding pipe 7 in real time, and the control device can control the pump device 2 to operate according to the detection result of the first detection member 8. Specifically, when it is detected that the functional liquid flows out from the other end of the filler pipe 7, indicating that the filler pipe 7 is filled with the liquid, the control device controls the pump device 2 to stop operating. Then the cell culture bottle to be filled with liquid is moved to the other end of the liquid adding pipeline 7, and then the pump device 2 is operated, so that the volume of the filled functional liquid can be accurately controlled.

It should be noted that, in practical applications, the first detecting member 8 may be set as a liquid detecting sensor, the liquid detecting sensor is set right below the other end of the liquid feeding pipe 7, when liquid flows out from the other end of the liquid feeding pipe 7, the liquid detecting sensor can just drop on the liquid detecting sensor, the liquid detecting sensor sends a specific signal to the control device after detecting the liquid, and the control device controls the pump device 2 to stop running after receiving the specific signal sent by the liquid detecting sensor; or, also can set up first detection component 8 into the camera, gather the image of the other end of liquid feeding pipeline 7 in real time through the camera to give controlling means with the image transmission, controlling means judges whether liquid flows through the other end that liquid feeding pipeline 7 was judged through carrying out analysis processes to the image, etc. this kind of adjustment and the change of the specific type of first detection component 8 do not deviate the utility model discloses a principle and scope all should be injectd within the protection scope of the utility model.

Preferably, the utility model discloses a liquid filling system is still including setting up second detection component (not shown in the figure) in the liquid feeding pipeline 7, the second detect the component with controlling means communication connection just can detect and be located the bubble in the liquid feeding pipeline 7.

Through detecting the component with the second and detecting the component 8 cooperation use, can be more accurate assurance filling pipe 7 intussuseption is full of functional liquid. In a particular application, the control device controls the pump device 2 to stop operating only when the first detection member 8 detects that there is an outflow of liquid at the other end of the filling duct 7 and there is no steam pocket in the functional liquid located in the filling duct 7. That is, even if the first detection means 8 detects the outflow of liquid at the other end of the filling duct 7, if the second detection means detects the presence of a steam pocket in the functional liquid located in the filling duct 7, the operation of the pump device 2 is continued until there is no steam pocket in the functional liquid located in the filling duct 7.

In practical applications, the second detecting member may be a bubble detecting sensor, such as an ultrasonic bubble detecting sensor, in which the density of the liquid is relatively stable under normal conditions without bubbles mixed therein, the ultrasonic wave is smoothly transmitted to the receiving end in the liquid, the signal has almost no large distortion, if bubbles exist in the liquid, the density of the liquid is reduced, the ultrasonic wave is scattered by the bubbles on the propagation path, and the energy received by the receiving end is attenuated and the signal waveform is distorted compared with the normal conditions; alternatively, other types of detecting members may be used, and such adjustments and changes to the specific type of the second detecting member do not depart from the scope of the present invention, and should be limited within the scope of the present invention.

Preferably, the liquid filling system of the present invention further comprises a third detecting member 9 disposed on the storage device 3, wherein the third detecting member 9 is in communication connection with the control device and can detect the weight of the functional liquid in the storage device 3.

By providing the third detection means 9 to detect the weight of the functional liquid in the storage device 3, when the functional liquid is insufficient, it is possible to prompt the user to replenish the functional liquid into the storage device 3 in time.

It should be noted that, in practical applications, a person skilled in the art may set the third detecting member 9 as a weighing sensor, and install the weighing sensor at the bottom of the storage device 3, or may set the third detecting member 9 as a pressure sensor, and install the pressure sensor at the bottom of the storage device 3, etc., and such adjustment and change of the specific type of the third detecting member 9 do not deviate from the scope of the present invention, and should be limited within the protection scope of the present invention.

Preferably, the utility model discloses a liquid filling system still includes refrigerating plant (not shown in the figure) and heating device, refrigerating plant sets up storage device 3 is last and can be right functional liquid in the storage device 3 cools down, heating device sets up on the liquid supply pipe 4 and can be right functional liquid in the liquid supply pipe 4 heats.

By providing the refrigeration device in the storage device 3, the storage device 3 has a low-temperature environment, generally about 4 degrees, and the storage time of the functional liquid can be prolonged.

It should be noted that the internal ambient temperature of the storage device 3 is not limited to the 4 degrees described above, for example, the internal ambient temperature of the storage device 3 may also be set to 3 degrees, 5 degrees, 8 degrees, or the like, and a person skilled in the art may flexibly set the internal ambient temperature of the storage device 3 according to experiments or experience as long as the storage time of the functional liquid can be prolonged.

It should also be noted that, in practical applications, those skilled in the art can set the refrigeration device as a refrigerant compressor refrigeration device, a semiconductor refrigeration device, etc., and this adjustment and change to the specific type of the refrigeration device do not deviate from the principle and scope of the present invention, which should be limited within the protection scope of the present invention.

Before the functional liquid is conveyed to the cell culture bottle, the functional liquid can be heated by a heating device arranged on the liquid supply pipeline 4, and exemplarily, the functional liquid can be heated to about 37 degrees so as to meet the requirement of cell culture.

In practical applications, a person skilled in the art can set the temperature range of the functional liquid according to the specific requirements of cell culture, which is not limited by the present invention.

Furthermore, it should be noted that, in practical applications, a person skilled in the art may install the storage device 3 inside the incubator 1, or may install the storage device 3 outside the incubator 1, and such adjustment and change of the specific installation position of the storage device 3 do not deviate from the principle and scope of the present invention, and should be limited within the protection scope of the present invention.

Preferably, as shown in FIG. 1, the cell culture workstation of the present invention installs the storage device 3 outside the incubator 1.

With will storage device 3 installs compare in the inside of incubator 1, through will storage device 3 installs the outside of incubator 1, on the one hand, when replenishing the functional liquid, need not to open incubator 1 to can avoid causing the pollution to the internal environment of incubator 1, on the other hand, can also avoid taking up the inner space of incubator 1.

Preferably, as shown in fig. 1, the heating device of the present invention comprises a heating box 5 and a heating member 6 installed on the heating box 5, a part of the liquid supply pipe 4 is located inside the heating box 5, a heating liquid is stored inside the heating box 5, the heating member 6 can heat the heating liquid to pass through the heating liquid to heat the functional liquid inside the liquid supply pipe 4.

It should be noted that, in practical applications, a person skilled in the art may set the heating member 6 as a heating wire, or may set the heating member 6 as a heating pipe, or may set the heating member 6 as a heating plate, and so on, and such adjustment and change of the specific structural form of the heating member 6 do not depart from the principle and scope of the present invention as long as the heating liquid in the heating box 5 can be heated by the heating member 6.

In addition, it should be noted that, in practical application, a person skilled in the art may also cancel the heating box 5, and directly wind the heating member 6 on the liquid supply pipeline 4 to heat the functional liquid in the liquid supply pipeline 4, and such adjustment and change of the specific structural form of the heating device do not deviate from the principle and scope of the present invention, which should be defined within the protection scope of the present invention.

Of course, preferably set up the structural style of "heating cabinet 5+ heating member 6" with heating device to the above-mentioned introduction, compare in directly winding heating member 6 on supply pipe 4, the utility model discloses an indirect functional liquid heating to in the supply pipe 4 of heating fluid for heating temperature is more stable, and heating effect is better.

Preferably, the heating member 6 is provided as a heating wire.

It should be noted that, in practical applications, a person skilled in the art may set the heating liquid as pure water, or may set the heating liquid as heating oil, etc., and such adjustment and change of the specific type of the heating liquid do not depart from the principle and scope of the present invention, as long as the functional liquid in the liquid supply pipeline 4 can be heated by the heating liquid.

Preferably, the utility model discloses will the heating fluid in heating cabinet 5 sets up to the pure water.

By using purified water as the heating liquid, the functional liquid in the liquid supply pipeline 4 can be prevented from being polluted.

Preferably, as shown in fig. 1, the liquid supply duct 4 inside the heating box 5 extends in a spiral configuration. By arranging the liquid supply pipeline 4 in the heating box 5 to be of a spiral structure, the contact area between the liquid supply pipeline 4 and a heating liquid can be increased, and the heating of the functional liquid in the liquid supply pipeline 4 is facilitated.

Preferably, the cell culture workstation of the present invention further comprises a temperature sensor (not shown in the figure) disposed in the heating chamber 5, wherein the temperature sensor is used for detecting the temperature of the heating liquid.

By providing a temperature sensor in the heating tank 5 to detect the temperature of the heated liquid, it is convenient to accurately control the temperature of the functional liquid.

Preferably, the heating box 5 of the present invention is provided with a heat insulating layer (not shown in the figure).

The heat preservation layer is arranged on the heating box 5, so that the temperature of the heating liquid in the heating box 5 is more favorably kept stable.

Finally, it should be noted that a plurality of functional liquids are required during the cell culture process, and in practical applications, a person skilled in the art may store a plurality of different functional liquids in the same storage device 3, in which case a plurality of independent storage boxes may be provided in the storage device 3, each storage box storing one functional liquid, and a separate liquid supply conduit 4, pump device 2 and liquid supply conduit 7 are provided for each functional liquid.

Further, in view of the above, one heating device may be provided for each liquid supply pipe 4, or a plurality of liquid supply pipes 4 may be heated by one heating device in common, and illustratively, a portion of each liquid supply pipe 4 may be mounted in a heating tank 5, and the functional liquid in the liquid supply pipe 4 may be heated by the heated liquid in the heating tank 5.

So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions can be made on the related technical features by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions will fall into the protection scope of the invention.

Claims (10)

1. A liquid filling system for a cell culture workstation is characterized by comprising a storage device, a liquid supply pipeline, a liquid adding pipeline, a pump device, a first detection component and a control device, wherein the storage device is used for storing functional liquid required by cell culture, one end of the liquid supply pipeline is communicated with the storage device, the other end of the liquid supply pipeline is communicated with an inlet end of the pump device, one end of the liquid adding pipeline is communicated with an outlet end of the pump device, the pump device and the first detection component are both in communication connection with the control device, the first detection component can detect liquid flowing out of the other end of the liquid supply pipeline, and the control device can control the pump device to act according to a detection result of the detection component.

2. The liquid filling system for a cell culture workstation of claim 1, further comprising a second detection member disposed within the priming conduit, the second detection member being communicatively coupled to the control device and capable of detecting air bubbles within liquid located within the priming conduit.

3. The liquid filling system for a cell culture workstation according to claim 1, further comprising a third detection member disposed on the storage device, the third detection member being communicatively coupled to the control device and capable of detecting the weight of the functional liquid located in the storage device.

4. The liquid filling system for the cell culture workstation according to claim 1, further comprising a refrigerating device and a heating device, wherein the refrigerating device is disposed on the storage device and can cool the functional liquid in the storage device, and the heating device is disposed on the liquid supply pipeline and can heat the functional liquid in the liquid supply pipeline.

5. The liquid filling system for a cell culture workstation of claim 4, wherein the heating device comprises a heating tank within which a portion of the liquid supply conduit is located and a heating member mounted on the heating tank, the heating tank storing a heated liquid therein, the heating member being capable of heating the heated liquid to heat the functional liquid within the liquid supply conduit with the heated liquid.

6. The liquid filling system for a cell culture workstation of claim 5, wherein the liquid supply conduit within the heating chamber extends in a spiral configuration.

7. The liquid filling system for a cell culture workstation of claim 5, further comprising a temperature sensor mounted within the heating chamber for detecting a temperature of the heated liquid.

8. The liquid filling system for the cell culture workstation according to claim 5, wherein an insulating layer is provided on the heating chamber.

9. The liquid filling system for a cell culture workstation of any one of claims 1 to 8, wherein the storage device is installed outside an incubator of the cell culture workstation.

10. A cell culture workstation comprising a liquid priming system according to any one of claims 1 to 9.

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