CN212438940U - Experimental mouse eye vitreous cavity injection needle - Google Patents
Experimental mouse eye vitreous cavity injection needle Download PDFInfo
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- CN212438940U CN212438940U CN202020168702.1U CN202020168702U CN212438940U CN 212438940 U CN212438940 U CN 212438940U CN 202020168702 U CN202020168702 U CN 202020168702U CN 212438940 U CN212438940 U CN 212438940U
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Abstract
The utility model discloses an injection needle for experimental mouse eye glass body cavity, include rubber suction ball, glass needle afterbody, glass needle head portion in proper order, its characterized in that, glass needle afterbody, glass needle head portion are hollow structure, and the tip of glass needle afterbody is the inclined plane. The utility model provides the high success rate of mouse eye vitreous body injection in the ophthalmic animal experiment absorbs 1 mu L injection through the superfine glass needle tubing of syringe needle somatic part, pierces and effectively injects behind the mouse eye, can improve the success rate of injection, reduces the error of injection dosage, improves the reliability of experimental result, can form standard experiment consumptive material.
Description
Technical Field
The invention relates to an injection needle for an eye vitreous cavity of an experimental mouse, which is used for injecting the eye vitreous cavity of a rat or a mouse in an animal experiment.
Background
The intravitreal injection is an important technical means for animal experiments in basic ophthalmic research, is used in many important basic research fields such as animal models of retinal neovascularization, choroidal neovascularization and the like, and is used for injecting molecules such as antibodies, proteins and microRNA to be researched into the vitreous body cavity of the eye through the intravitreal injection, and further verifying the in vivo effect of the molecules by observing the change of the area of the retinal neovascularization. However, the volume of the eye of the mouse is small, the injection amount of the vitreous cavity is very small, and is only 1 muL, so that the requirement on the accuracy of the injection amount is very high, and the injection dosage error caused by the residue of liquid in the injection needle cannot exist, thereby influencing the experimental result.
The common clinical syringe that uses in the experiment, including 1mL syringe and insulin syringe, because the condition such as syringe design can lead to the injection liquid to remain in the syringe head by a small amount, and produce the bubble easily, lead to the error of injection dosage, the syringe needle is too thick to the rat eye simultaneously, can lead to the injection liquid to flow back in the eye, influences the reliability of experimental result.
Chinese patent document CN 209154192U, application date 2019.07.26, with patent names: the utility model provides a special syringe of operation and syringe needle of ophthalmology vitreous cavity injection art, discloses a special syringe of operation and syringe needle of ophthalmology vitreous cavity injection art, including the syringe lumen, the outside symmetry of syringe lumen one end is provided with the fixed block, and the inside of syringe lumen is provided with second rubber ejector pad, the intermediate position department that second rubber ejector pad is close to fixed block one side is provided with the push rod, and the one end that the second rubber ejector pad was kept away from to the push rod is provided with the pressing block. This syringe needle is provided with a little square lug in the place apart from needle point 3.5mm, the front end distance of lug is 3.5mm from the needle point, the trailing edge is apart from the needle point 4mm for the operation in-process, utilize the lug can accurate needle feeding position, the operation link has been reduced, do not need assistant's volume to get the position of needle feeding, the human input has been reduced, and need not use special ophthalmology measuring tape, avoid leading to needle feeding positional deviation because of the precision problem of measuring tape, improve the operation precision. The injection needle has the defects that the injection needle is mainly used for vitreous cavity injection of clinical patients, is too large, is not suitable for rat eyes, cannot accurately and finely quantify to 1 mu L, and cannot be used for ophthalmic animal experiments.
Chinese patent document CN 101732792a, application date 2010.06.16, with patent names: a medical vitreous cavity injector is disclosed, which is provided with a three-way pipe, two built-in one-way valves and two 1mL injectors. The single pipe end of the three-way pipe is connected with a 30G injection needle, the double pipe ends are respectively connected with two 1mL injection needle pipes through built-in one-way valves, and the control flow directions of the two one-way valves are respectively opposite and enter the three-way pipe. The technical scheme disclosed by the patent is mainly used for intravitreal injection of clinical patients, the needle head is too large, the needle head is not suitable for rat eyes, accurate and fine quantification cannot be achieved to 1 mu L, and the needle head cannot be used for ophthalmic animal experiments.
In summary, there is a need for a simple, practical, convenient, and accurate dosage injection needle for mouse eye vitreous cavity to reduce the error of injection dosage and improve the reliability of experimental results.
Disclosure of Invention
The utility model discloses the technical problem that will solve is: provided is an injection needle for vitreous cavity of eye of an experimental mouse, which is easy to insert and accurate in dosage.
In order to solve the technical problem, the technical scheme of the utility model is that: the injection needle for the vitreous body cavity of the mouse eye for the experiment sequentially comprises a rubber suction ball, a glass needle tail part and a glass needle head part, and is characterized in that the glass needle tail part and the glass needle head part are both of a hollow structure, and the end part of the glass needle tail part is an inclined plane.
Preferably, the volume of the glass tip segment is not less than 1 μ L.
More preferably, the surface of the glass needle head part is provided with 1 μ L scale mark.
Preferably, the length of the glass tip segment is 5cm and the outer diameter is 0.5 mm.
The sharp head of the glass needle is beneficial to easily penetrate into the vitreous cavity, the body is slender and can contain about 1 mu L of liquid, the body is provided with 1 mu L of scale marks, the tail part of the glass needle is used for being connected with a rubber suction ball, and the tail rubber suction ball is used for helping to suck 1 mu L of injection liquid and inject the injection liquid into the vitreous cavity.
The utility model discloses a head is sharp, easily pierces rat eye vitreous body chamber, and syringe needle somatic part is superfine, and is less to the wound of rat eye, does benefit to the restoration of rat eye and prevents to infect, and needle body portion can hold about 1 uL liquid simultaneously, and difficult gassing or the incomplete scheduling problem of injection make experimental operation accurate, and the data that is favorable to the experiment in later stage to observe and experiment are accurate, helps ophthalmology's basic scientific research work.
The utility model discloses being the important technological means of animal experiment among the ophthalmology basic research in view of vitreous cavity injection, all can using in many important basic research fields such as retina neovascular, animal models such as choroid neovascular, inject the molecule such as antibody, protein, microRNA that will study into the vitreous body intracavity through vitreous cavity injection, and then verify the internal effect of molecule through observing the change of retina neovascular area. However, the mouse eye is very small, the intraocular volume is small, the injection amount of the vitreous cavity is very small, and is only 1 muL, so that the requirement on the accuracy of the injection amount is very high, and the injection dosage error caused by the residue of liquid in the injection needle cannot exist, thereby influencing the experimental result. At present, the common clinical syringes used in the experiment include a 1mL syringe and an insulin syringe, and due to the design of the syringe and other situations, a small amount of injection liquid remains on the head of the syringe, which causes errors of injection dosage, and the needle head is too thick for the rat eye, which causes the injection liquid to flow back out of the eye, which affects the credibility of the experiment result.
The utility model provides the high success rate of mouse eye vitreous body injection in the ophthalmic animal experiment absorbs 1 mu L injection through the superfine glass needle tubing of syringe needle somatic part, pierces and effectively injects behind the mouse eye, can improve the success rate of injection, reduces the error of injection dosage, improves the reliability of experimental result, can form standard experiment consumptive material.
Drawings
Fig. 1 is a schematic view of the injection needle for vitreous body cavity of laboratory mouse eye provided by the utility model.
Detailed Description
In order to make the present invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.
Examples
As shown in fig. 1, the injection needle for a vitreous cavity of an experimental mouse eye provided by this embodiment is an experimental consumable, and sequentially includes a rubber suction ball 3, a glass needle tail portion 1, and a glass needle head portion 2, where the glass needle head portion 2 is a hollow structure and has an end portion that is an inclined plane.
The volume of the glass needle head part 2 is not less than 1 muL, and the surface of the glass needle head part 2 is provided with 1 muL scale marks 4.
The length of the glass needle head part 2 is 5cm, and the outer diameter is 0.5 mm.
The glass needle head part 2 has a sharp slope, is beneficial to easily penetrate into a vitreous cavity, is slender and can contain about 1 mu L of liquid, the body part is provided with scale marks of 1 mu L, the length of the glass needle tail part 1 is 3cm and is used for being connected with the rubber suction ball 3, and the rubber suction ball at the tail part is used for helping to suck 1 mu L of injection liquid and inject the injection liquid into the vitreous cavity.
The experimental mouse eye vitreous cavity injection needle has the advantages that an experimental operator can firstly use a 1 mu L liquid transfer gun to suck 1 mu L of injection liquid, then the injection liquid is dripped on a sterilized glass sheet to form liquid drops, then the injection needle is taken, a rubber suction ball is pinched to form negative pressure and then is aligned to the injection liquid drops, the rubber suction ball is loosened slowly to enable the injection liquid to be sucked into the body of the injection needle to 1 mu L scale, the rubber suction ball is fixed and not loosened, the head part of the glass needle is slowly punctured into the mouse eye vitreous cavity, the rubber suction ball is pinched after the glass needle enters the vitreous cavity, the injection liquid enters the mouse eye vitreous cavity, and the experimental operation is completed.
Claims (4)
1. The utility model provides an injection needle of experiment mouse eye vitreous cavity, includes rubber suction ball (3), glass needle afterbody (1), glass needle head portion (2) in proper order, its characterized in that, glass needle afterbody (1), glass needle head portion (2) are hollow structure, and the tip of glass needle afterbody (1) is the inclined plane.
2. The experimental mouse vitreous cavity injection needle according to claim 1, wherein the volume of the glass tip section (2) is not less than 1 μ L.
3. The experimental mouse vitreous cavity injection needle according to claim 2, wherein the surface of the glass tip section (2) is provided with 1 μ L of graduation marks (4).
4. The experimental mouse vitreous cavity injection needle according to claim 1, wherein the glass tip segment (2) has a length of 5cm and an outer diameter of 0.5 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020168702.1U CN212438940U (en) | 2020-02-13 | 2020-02-13 | Experimental mouse eye vitreous cavity injection needle |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020168702.1U CN212438940U (en) | 2020-02-13 | 2020-02-13 | Experimental mouse eye vitreous cavity injection needle |
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| Publication Number | Publication Date |
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| CN212438940U true CN212438940U (en) | 2021-02-02 |
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| CN202020168702.1U Active CN212438940U (en) | 2020-02-13 | 2020-02-13 | Experimental mouse eye vitreous cavity injection needle |
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2020
- 2020-02-13 CN CN202020168702.1U patent/CN212438940U/en active Active
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