CN212418338U - Perforated plate liquid treatment device adaptive to centrifugal machine - Google Patents
Perforated plate liquid treatment device adaptive to centrifugal machine Download PDFInfo
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- CN212418338U CN212418338U CN202020936479.0U CN202020936479U CN212418338U CN 212418338 U CN212418338 U CN 212418338U CN 202020936479 U CN202020936479 U CN 202020936479U CN 212418338 U CN212418338 U CN 212418338U
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- 239000007788 liquid Substances 0.000 title claims abstract description 200
- 230000003044 adaptive effect Effects 0.000 title abstract description 4
- 238000000034 method Methods 0.000 claims description 8
- 230000003746 surface roughness Effects 0.000 claims description 8
- 238000010276 construction Methods 0.000 claims 1
- 238000002474 experimental method Methods 0.000 abstract description 15
- 238000005119 centrifugation Methods 0.000 description 9
- 238000006243 chemical reaction Methods 0.000 description 9
- 230000006978 adaptation Effects 0.000 description 5
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Abstract
The utility model provides a porous plate liquid processing device adaptive to a centrifuge, which comprises an opening frame, a liquid collecting component and a liquid storage tank; the mouth-shaped frame is in a mouth-shaped structure, and the cross section of the mouth-shaped frame is a four-side ring; a liquid collecting component is arranged between the mouth-shaped frame and the liquid storage tank; the liquid collecting assembly forms a funnel structure in the mouth-shaped frame; during application, the space position of the liquid collecting assembly is higher than that of the liquid storage tank. Through the utility model discloses a perforated plate liquid processing apparatus, can with in the experiment need remove wherein the perforated plate back-off of liquid in perforated plate liquid processing apparatus among, carry out centrifugal operation for liquid reverse movement reaches the open end of the inside reservoir of perforated plate.
Description
Technical Field
The utility model belongs to centrifugate processing apparatus field, concretely relates to perforated plate liquid processing apparatus of centrifuge adaptation.
Background
The collection or disposal of the centrifugation of liquids in multi-well plates is a common experimental operation in the performance of biological experiments. Currently, methods of collecting or disposing of liquids in multi-well plates include: and sucking the liquid one by using a single-channel liquid shifter and discarding the liquid, or sucking the liquid one by using a multi-channel liquid shifter and discarding the liquid, or sucking the liquid by using a vacuum pump suction system and a suction nozzle and discarding the liquid, or manually throwing the liquid in the porous plate.
However, these pipetting methods all have different defects, and when the single-channel pipettor is used for performing the liquid pipetting step one by one, the throughput is low, the time consumption is long, and consumable materials such as pipette tips and the like are consumed. For a multi-channel pipette, a large amount of consumables such as a sucker and the like are still consumed in a liquid disposal device, and the multi-channel pipette is more suitable for a multi-hole plate with 96 holes and the like and is not suitable for a multi-hole plate with 384 holes and higher holes; in the process of imbibing, liquid is easy to remain; the bottom and the wall of the reaction hole are easy to scratch, and the experiment result is obviously influenced. In reactions using multi-well plates, such as plate-bottom cell culture and detection, affinity capture reaction and enzyme-linked immunosorbent assay, a liquid reaction system in a well is combined with adsorbed substances on the bottom and wall of the well, continuous multi-step operations including liquid feeding, well washing, reaction liquid collection and the like are required, and a suction head of a multi-channel pipette is easy to scratch on molecules or biological structures captured on the wall and bottom of the well when liquid is removed, so that the reaction system is changed, the read quantitative result is inaccurate, and finally the reliability reported by a detection laboratory is reduced. For a vacuum suction system, liquid is easy to remain in a pipeline, and the pipeline has higher pollution risk after being used for multiple times.
The above prior art has the technical problems which are difficult to solve: in the biological experiment process, flux, operation time and consumable consumption cannot be taken into consideration, the method of using a pipettor and vacuum suction cannot ensure that the reaction hole wall is not damaged, the manual spin-drying cannot ensure that the reaction holes are not cross-contaminated, and the defects further cannot ensure the accuracy of results, and even cannot be applied to the flexible switching of liquid collection and separation of the hole plates.
SUMMERY OF THE UTILITY MODEL
For solving the problem that prior art exists, the utility model provides a perforated plate liquid treatment device of centrifuge adaptation can with in the experiment need remove wherein the perforated plate back-off of liquid in perforated plate liquid treatment device among, carry out centrifugal operation for liquid reverse movement reaches the open end of the inside stock solution pipe of perforated plate.
The utility model provides a porous plate liquid processing device adaptive to a centrifuge, which comprises an opening frame, a liquid collecting component and a liquid storage tank; the mouth-shaped frame is in a mouth-shaped structure, and the cross section of the mouth-shaped frame is a four-side ring; a liquid collecting component is arranged between the mouth-shaped frame and the liquid storage tank; the liquid collecting assembly forms a funnel structure in the mouth-shaped frame; during application, the space position of the liquid collecting assembly is higher than that of the liquid storage tank. Through this technical scheme, can be used to in the experiment need remove wherein the perforated plate back-off of liquid in the perforated plate liquid processing apparatus among, carry out centrifugal operation for the liquid reverse movement is to the open end of the liquid storage pipe of perforated plate inside.
Further, be porous plate liquid processing apparatus, the collection liquid subassembly include collection liquid board and collection liquid channel, collect liquid board with the contained angle scope of the inside side of mouth type frame is 95~170, collection liquid channel be located between the adjacent collection liquid board. Through this technical scheme, need remove among wherein the perforated plate back-off of liquid in the experiment in the perforated plate liquid processing apparatus, when carrying out centrifugal operation, can collect the perforated plate liquid fast in the reservoir of bottom.
Further, for the porous plate liquid treatment device, the porous plate liquid treatment device further comprises a porous plate fixing device, and the porous plate fixing device is of an annular structure; in the application process, the shape of the longitudinal section of the orifice plate fixing device is an inverted trapezoid. Through this technical scheme, need remove among wherein the perforated plate back-off of liquid in the experiment in the perforated plate liquid processing apparatus, when carrying out centrifugal operation, can collect the perforated plate liquid fast in the reservoir of bottom.
Further, for the porous plate liquid treatment device, the surface roughness Ra of the liquid collecting component ranges from 0.1 to 10 μm. Through this technical scheme, need remove among wherein the perforated plate back-off of liquid in the experiment in the perforated plate liquid processing apparatus, when carrying out centrifugal operation, can collect the perforated plate liquid fast in the reservoir of bottom.
Further, for the porous plate liquid treatment device, the liquid collecting assembly is symmetrically arranged at the side part of the liquid storage tank by taking the center of the liquid storage tank as a symmetrical point. Through this technical scheme, need remove among wherein the perforated plate back-off of liquid in the experiment in the perforated plate liquid processing apparatus, when carrying out centrifugal operation, can collect the perforated plate liquid fast in the reservoir of bottom.
Further, in the liquid handling apparatus for a multi-well plate, the well plate holding means is a quadrangular ring structure, a circumference of a top cross section of the well plate holding means is larger than a circumference of a bottom cross section by at least 1mm, and a surface roughness Ra of the well plate holding means ranges from 0.1 to 10 μm. Through the technical scheme, the porous plate which needs to remove liquid in the experiment is reversely buckled in the porous plate liquid treatment device, and the porous plate can be easily taken out after the centrifugal operation is completed.
Further, for the porous plate liquid treatment device, the mouth-shaped frame, the liquid collection assembly and the liquid storage tank are of an integrally formed structure. Through this technical scheme, need remove among wherein the perforated plate back-off of liquid in the experiment in the perforated plate liquid processing apparatus, when carrying out centrifugal operation, can collect the perforated plate liquid fast in the reservoir of bottom.
Further, as for the perforated plate liquid treatment apparatus, the perforated plate liquid treatment apparatus further includes a holder ring, the holder ring is located at the bottom of the mouth frame, and the circumference of the cross section of the holder ring is smaller than the circumference of the cross section of the top end of the perforated plate fixing apparatus. Through this technical scheme, can make things convenient for the stack of porous plate liquid treatment facilities stack.
Further, to the perforated plate liquid treatment device, the perforated plate liquid treatment device further comprises a support ring, the support ring is located between the perforated plate fixing device and the liquid collecting assembly, and the support ring is connected with the perforated plate fixing device through an arc-shaped angle. Through the technical scheme, the porous plate which needs to remove liquid in the experiment is reversely buckled in the porous plate liquid treatment device, and the porous plate can be easily taken out after the centrifugal operation is completed.
The utility model discloses the beneficial effect who creates: the utility model provides a perforated plate liquid processing apparatus of centrifuge adaptation, can with in the experiment need remove wherein the perforated plate back-off of liquid in perforated plate liquid processing apparatus among, carry out centrifugal operation for liquid reverse movement realizes following function to the open end in hole:
a) liquid disposal: under the condition of not touching the reaction surface in the hole, the liquid in the porous plate is discarded, so that the liquid residue in the porous plate is ensured to be as little as possible;
b) liquid collection: without touching the reaction surface in the well, the liquid in the porous plate is collected, ensuring the complete recovery of the required reaction liquid in the porous plate.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from these drawings without inventive effort.
FIG. 1 is a top view of an accumulator in external appearance;
FIG. 2 is a top view of an accumulator;
FIG. 3 is a perspective view of a liquid trap;
FIG. 4 is a rear elevational view of a liquid trap;
FIG. 5 is an external view of a liquid trap.
Detailed Description
The experimental methods of the following examples, which are not specified under specific conditions, are generally determined according to national standards. If there is no corresponding national standard, it is carried out according to the usual international standards, to the conventional conditions or to the conditions recommended by the manufacturer.
The features mentioned in the present invention or in the embodiments can be combined. All the features disclosed in this specification may be combined in any combination, and each feature disclosed in this specification may be replaced by alternative features serving the same, equivalent or similar purpose. Thus, unless expressly stated otherwise, the features disclosed are merely generic examples of equivalent or similar features.
In the present invention, all the technical features mentioned herein and preferred features may be combined with each other to form a new technical solution, if not specifically stated.
In order to make the technical means, the creation features, the achievement purposes and the functions of the present invention easy to understand, the present invention will be further explained with reference to the following embodiments, but the present invention includes but is not limited to these embodiments.
EXAMPLE 1 centrifuge-adapted perforated plate liquid treatment apparatus
This example describes primarily a centrifuge adapted multi-well plate liquid handling device.
Referring to fig. 1, the porous plate liquid processing device adapted to the centrifuge comprises an opening frame 1, a liquid collecting plate 2 and a liquid storage tank 4; preferably, the die frame 1, the liquid collecting plate 2 and the liquid storage tank 4 are of an integrally formed structure. The mouth-shaped frame 1 is of a 'mouth' -shaped structure, the cross section of the mouth-shaped frame is a four-side ring, the annular width of the cross section of the mouth-shaped frame 1 can be set between 0.1-5 cm, and the mouth-shaped frame 1 is used for fixing and supporting the liquid collecting plate 2. A liquid collecting plate 2 is arranged in the mouth-shaped frame 1 and is used for collecting liquid collected in the centrifugal process into a liquid storage tank 4 along the liquid collecting plate; the liquid collecting plate 2 comprises a liquid collecting plate 21, a liquid collecting plate 22, a liquid collecting plate 23 and a liquid collecting plate 24, and the liquid collecting plate 21, the liquid collecting plate 22, the liquid collecting plate 23 and the liquid collecting plate 24 form a funnel structure in the mouth-shaped frame 1. A liquid collecting plate 21 and a liquid collecting plate 23 having the same shape and configuration, both having a surface roughness Ra in the range of 0.008 to 10 μm (preferably not more than 0.1 μm), both disposed on both sides of the reservoir 4 in a manner of being centrosymmetric with respect to the center of the reservoir 4; a liquid collecting plate 22 and a liquid collecting plate 24 having the same shape and configuration, both having a surface roughness in the range of 0.008 to 10 μm (preferably not more than 0.1 μm), both being disposed on both sides of the reservoir 4 in a manner of being centrosymmetric with respect to the center of the reservoir 4; the drip plate 21, the drip plate 22, the drip plate 23 and the drip plate 24 are connected to the side of the reservoir 4 in a seamless abutting manner. The cross-section of the reservoir 4 comprises a circle, but the cross-section of the reservoir 4 may also be irregular or polygonal. The reservoir 4 is a liquid storage component for collecting liquid during centrifugation. During application, the liquid collecting plate 2 is positioned at a higher spatial position than the liquid storage tank 4.
Referring to fig. 2, the liquid treatment device with a perforated plate adapted to the centrifuge further includes a liquid collecting channel 3, the structure of the liquid collecting channel 3 includes, but is not limited to, a concave groove structure of a semicircular arc body, one end of the liquid collecting channel 3 is connected with the side edge of the mouth-shaped frame 1, and the other end is connected with the liquid storage tank 4, and the liquid is collected into the liquid storage tank 4 through the liquid collecting channel 3 after centrifugation. The liquid collecting channel 3 includes a liquid collecting channel 31, a liquid collecting channel 32, a liquid collecting channel 33, and a liquid collecting channel 34. The liquid collecting plate 21 and the liquid collecting plate 22 are connected through a liquid collecting channel 31, the liquid collecting plate 22 and the liquid collecting plate 23 are connected through a liquid collecting channel 32, the liquid collecting plate 23 and the liquid collecting plate 24 are connected through a liquid collecting channel 33, and the liquid collecting plate 24 and the liquid collecting plate 21 are connected through a liquid collecting channel 34. The surface roughness Ra of the liquid collection channel 3 is in the range of 0.008-10 μm (preferably not more than 0.1 μm).
Referring to fig. 3, the mouth frame 1 further includes a support ring 5, a support ring 6 and a side ring 7, the support ring 5 is a four-sided ring and is located at the bottom of the mouth frame 1, and the circumference of the cross section of the support ring 5 is smaller than the circumference of the cross section of the top end of the side ring 7, so as to stack the porous plate liquid processing device adapted to the centrifuge. The support ring 6 is a four-side ring, the support ring 6 is positioned between the side ring 7 and the liquid collecting component 2, and the support ring 6 and the side ring 7 are connected by an arc angle or a right angle; the support ring 6 is used to support a centrifuge plate (including but not limited to one of a 6-well plate, a 12-well plate, a 24-well plate, a 48-well plate, a 96-well plate, a 384-well plate, or a 1536-well plate). The side ring 7 is a four-side ring, the cross section of the side ring 7 is a four-side ring, the perimeter from the cross section at the top end of the side ring 7 to the cross section at the bottom end of the side ring 7 is in a continuously decreasing trend, the perimeter of the cross section at the top end of the side ring 7 is at least 1mm greater than that of the cross section at the bottom end of the side ring 7, and the longitudinal section of the side ring 7 is trapezoidal; the side ring 7 is connected to the collector plate 2 by a support ring 6, the side ring 7 having a surface roughness Ra in the range of 0.008-10 μm (preferably no more than 0.1 μm), the side ring 7 being used to secure a well plate assembly for liquid collection or disposal.
Referring to fig. 2 and 3, the angle between the plane of the liquid collecting plate 21 (or the liquid collecting plate 23) and the nearest adjacent plane on the side ring 7 is 95-170 °.
Referring to fig. 4, the bottom of the perforated plate liquid processing device adapted to the centrifuge of the present invention is provided with a plurality of set squares 8, which are used to support the centrifugal force borne by the liquid collecting plate 2 during the centrifugation process, so as to prolong the stability and durability of the liquid collecting plate 2.
When using, combine to show with fig. 1 to 5, will the perforated plate liquid processing apparatus of centrifuge adaptation install on centrifuge's horizontal rotor, before centrifugation, with the perforated plate (including but not limited to 6 orifice plates, 12 orifice plates, 24 orifice plates, 48 orifice plates, 96 orifice plates, 384 orifice plates or 1536 orifice plates) back-off the inboard of centrifuge adaptation's perforated plate liquid processing apparatus's mouth type frame 1, begin centrifugation, because the cross section girth of side ring 7 constantly reduces, consequently can constantly lock the orifice plate in centrifugation process, liquid on the centrifuging tube in the orifice plate simultaneously can assemble reservoir 4 along collecting liquid board 2 and collecting liquid passageway 3, after the centrifugation is accomplished, takes off the orifice plate and accomplishes the separation of the inside centrifuging liquid supernatant of orifice plate and deposit, avoids adopting the drawback that can't effectively remove the supernatant of centrifugation among the traditional approach, And scratch and rub and experimental pollution caused by touching the inner wall of the centrifugal pipe.
The basic principles and the main features of the invention and the advantages of the invention have been shown and described above. It will be understood by those skilled in the art that the present invention is not limited to the above embodiments, and that the foregoing embodiments and descriptions are provided only to illustrate the principles of the present invention without departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (9)
1. The porous plate liquid treatment device matched with the centrifuge is characterized by comprising an opening frame, a liquid collecting assembly and a liquid storage tank;
the mouth-shaped frame is in a mouth-shaped structure, and the cross section of the mouth-shaped frame is a four-side ring;
a liquid collecting component is arranged between the mouth-shaped frame and the liquid storage tank; the liquid collecting assembly forms a funnel structure in the mouth-shaped frame;
during application, the space position of the liquid collecting assembly is higher than that of the liquid storage tank.
2. A porous plate liquid treatment device according to claim 1, wherein said liquid collection assembly comprises liquid collection plates and liquid collection channels, the included angle between said liquid collection plates and the inner side of said die frame is 95-170 °, and said liquid collection channels are located between adjacent liquid collection plates.
3. The multi-well plate liquid handling device according to claim 1, further comprising a well plate fixing device having a ring structure; in the application process, the shape of the longitudinal section of the orifice plate fixing device is an inverted trapezoid.
4. A porous plate liquid treatment apparatus according to claim 1, wherein said liquid collection member has a surface roughness Ra in the range of 0.1 to 10 μm.
5. A multi-well plate liquid handling device according to claim 1, wherein said liquid trap assembly is symmetrically disposed at the side of said reservoir with the center of said reservoir as a symmetry point.
6. The multi-well plate liquid handling device according to claim 3, wherein said well plate holding means is a quadrilateral ring structure, a circumference of a top cross section of said well plate holding means is larger than a circumference of a bottom cross section by at least 1mm, and a surface roughness Ra of said well plate holding means is in the range of 0.1 to 10 μm.
7. A multi-well plate liquid handling device according to claim 3 wherein said mouth frame, liquid collection assembly and reservoir are of an integrally formed construction.
8. The multi-well plate liquid handling device of claim 6, further comprising a support ring, said support ring being located at the bottom of said frame, said support ring having a cross-section with a smaller circumference than a top cross-section of said well plate fixture.
9. The perforated plate liquid management apparatus of claim 6 further comprising a support ring positioned between the perforated plate fixture and the manifold assembly, wherein the support ring is connected to the perforated plate fixture at an arcuate corner.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020936479.0U CN212418338U (en) | 2020-05-28 | 2020-05-28 | Perforated plate liquid treatment device adaptive to centrifugal machine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020936479.0U CN212418338U (en) | 2020-05-28 | 2020-05-28 | Perforated plate liquid treatment device adaptive to centrifugal machine |
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| Publication Number | Publication Date |
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| CN212418338U true CN212418338U (en) | 2021-01-29 |
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| CN202020936479.0U Active CN212418338U (en) | 2020-05-28 | 2020-05-28 | Perforated plate liquid treatment device adaptive to centrifugal machine |
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Effective date of registration: 20240311 Address after: 200000 Building 1, No.1 Haikun Road, Fengxian District, Shanghai Patentee after: Shanghai Mima Life Science Co.,Ltd. Country or region after: China Address before: 518000 room 1701, building B2, zone B, phase II, Baoneng Science Park (South District), Qinghu Industrial Zone, Gangtou community, Bantian street, Longgang District, Shenzhen City, Guangdong Province Patentee before: Shenzhen Bi Code Technology Co.,Ltd. Country or region before: China |