CN212413963U - Simple continuous inoculator for blade vascular bundle - Google Patents
Simple continuous inoculator for blade vascular bundle Download PDFInfo
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- CN212413963U CN212413963U CN202020442857.XU CN202020442857U CN212413963U CN 212413963 U CN212413963 U CN 212413963U CN 202020442857 U CN202020442857 U CN 202020442857U CN 212413963 U CN212413963 U CN 212413963U
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- fixed sleeve
- vascular bundle
- inoculator
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Abstract
The utility model provides a simple continuous inoculator for a blade vascular bundle, which comprises a liquid transferring device, wherein the front end of the liquid transferring device is provided with a connecting head; one end of the fixed sleeve is detachably sleeved outside the connector; the needle tube is fixed at the other end of the fixed sleeve and communicated with the fixed sleeve, a needle head is arranged at the other end, far away from the fixed sleeve, of the needle tube, the needle point of the needle head has a certain width, and the included angle between the needle head and the needle tube is 90-180 degrees. The utility model discloses can realize absorbing a fungus liquid and can puncture inoculation blade vascular bundle in succession many times, need not frequently to dip in the fungus, practice thrift operating time, improve inoculation speed by a wide margin.
Description
[ technical field ] A method for producing a semiconductor device
The utility model relates to a simple and easy continuous inoculator of blade vascular bundle.
[ background of the invention ]
Sweet potato blast, the pathogen of which is sweet potato Ralstonia Solanacearum, can cause destructive attack to sweet potato production areas in south China, the yield of sweet potatoes in diseased fields generally loses 20-30%, the serious sweet potatoes can reach 70-80%, and even the sweet potatoes are completely harvested, so the sweet potato blast is classified as sweet potato epidemic disease in China. In production, especially in high-incidence areas of the potato blast bacteria, the disease is prevented by breeding a disease-resistant variety of the potato blast bacteria. A key step in the process of breeding the disease-resistant variety is to inoculate the variety with the potato blast germs to carry out disease resistance identification. The indoor inoculation method of the potato plague bacteria is a leaf pricking method: (1) culturing CSFV, inoculating purified CSFV into sterile PSA liquid culture medium, shaking at 28 deg.C for 16 hr, measuring bacterial concentration, and adjusting bacterial liquid OD600The value is 0.8-1.0 for standby; (2) dipping bacteria liquid by using a sterile scalpel to puncture the main veins of the sweet potato leaves, wherein the puncture position is close to the leaf apex and is about 1/5 leaves away from the leaf apex; (3) moistening after inoculation, spray humidifying after inoculation, placing in an illumination incubator at 28 deg.C and 9 deg.C5% humidity, 16h light/8 h dark light cycle culture. Because each leaf needs to be dipped with the bacterial liquid again before puncturing each leaf in order to ensure that each leaf contacts with enough bacterial liquid, repeated bacterial dipping and puncturing consume time, and the inoculation efficiency is low.
[ Utility model ] content
The to-be-solved technical problem of the utility model lies in providing a simple and easy continuous inoculator of blade vascular bundle, can realize absorbing a fungus liquid and can puncture inoculation blade vascular bundle many times in succession, need not frequently to dip in the fungus, practices thrift operating time, improves inoculation speed by a wide margin.
The utility model discloses a realize like this:
a simple continuous inoculator for a blade vascular bundle comprises
The front end of the liquid transferring device is provided with a connecting head;
one end of the fixed sleeve is detachably sleeved outside the connector;
the needle tube is fixed at the other end of the fixed sleeve and communicated with the fixed sleeve, the other end of the needle tube, which is far away from the fixed sleeve, is provided with a needle head, and the needle point of the needle head has a certain width.
Furthermore, the included angle between the needle head and the needle tube is 90-180 degrees.
Furthermore, the fixed sleeve is internally provided with a clamping part.
Furthermore, the needle head is V-shaped, and the angle of the included angle is 45-180 degrees.
Furthermore, the width of the needle point is 0.2-0.5 cm.
The utility model has the advantages that:
(1) the leaf vascular bundle can be continuously punctured and inoculated for multiple times by sucking once bacterial liquid, frequent bacterium dipping is not needed, the operation time is saved, and the inoculation speed is greatly improved; (2) the utility model has simple structure and reasonable design, the designed needle point has a certain width and can easily cut off the blade, and the rapid and accurate puncture inoculation of the blade vascular bundle can be realized; in a word, the method has the characteristics of simple and easy operation, low cost and obvious effect.
[ description of the drawings ]
The invention will be further described with reference to the following examples with reference to the accompanying drawings.
FIG. 1 is a schematic view of the simple continuous inoculator for the blade vascular bundle of the present invention.
Fig. 2 is a front view of the engagement of the locking collar with the needle cannula of the present invention.
Figure 3 is a side view of the needle cannula and needle of the present invention.
Fig. 4 is a front view of the needle of the present invention.
[ detailed description ] embodiments
Referring to fig. 1 to 4, the present invention relates to a simple continuous inoculator for a blade vascular bundle, which comprises a liquid transferring device 1, wherein a connecting head 2 is arranged at the front end of the liquid transferring device 1;
a fixed sleeve 3, wherein one end of the fixed sleeve 3 is detachably sleeved outside the connector 2, and the fixed sleeve 3 is hermetically connected with the outside of the connector 2;
a needle tubing 4, needle tubing 4 is fixed the other end of adapter sleeve 3, just needle tubing 4 with adapter sleeve 3 is linked together, needle tubing 4 keeps away from adapter sleeve 3's the other end is provided with syringe needle 5, the needle point 6 of syringe needle 5 has certain width, is convenient for pierce through the blade vascular bundle.
The included angle alpha between the needle head 5 and the needle tube 4 is 90-180 degrees.
The inside block portion that is provided with of fixed sleeve 3 for fixed sleeve 3 better block is fixed in the connector 2 outside, and the connection of the two is inseparabler firm.
The needle head 5 is V-shaped, and the angle beta at the included angle is 45-180 degrees.
The width of the needle point 6 is 0.2-0.5 cm.
For better understanding of the above technical solutions, the following detailed descriptions will be provided in conjunction with the drawings and the detailed description of the embodiments.
The installation process of this embodiment is as follows: firstly, sucking a bacterial liquid by a pipettor 1; then the fixed sleeve 3 is sleeved and clamped on the connector 2; slightly pressing the pipettor 1 until the bacteria liquid flows out of the connector 2 and flows to the needle point 6 of the needle head 5 along the needle tube 4 through the fixed sleeve 3; puncturing the vascular bundle of the leaves by using the needle point 6 of the needle head 5 to complete the inoculation of the leaves; the pressure is increased and the pipettor 1 is lightly pressed to enable the bacteria liquid to continuously flow out to the needle point 6 of the needle head 5, so that continuous blade inoculation is realized.
The utility model has the advantages of it is following:
(1) the leaf vascular bundle can be continuously punctured and inoculated for multiple times by sucking once bacterial liquid, frequent bacterium dipping is not needed, the operation time is saved, and the inoculation speed is greatly improved; (2) the utility model has simple structure and reasonable design, the designed needle point has a certain width and can easily cut off the blade, and the rapid and accurate puncture inoculation of the blade vascular bundle can be realized; in a word, the method has the characteristics of simple and easy operation, low cost and obvious effect.
Although specific embodiments of the present invention have been described, it will be understood by those skilled in the art that the specific embodiments described are illustrative only and are not limiting upon the scope of the invention, and that equivalent modifications and variations can be made by those skilled in the art without departing from the spirit of the invention, which is to be limited only by the claims appended hereto.
Claims (5)
1. The utility model provides a simple and easy continuous inoculator of blade vascular bundle which characterized in that: comprises that
The front end of the liquid transferring device is provided with a connecting head;
one end of the fixed sleeve is detachably sleeved outside the connector;
the needle tube is fixed at the other end of the fixed sleeve and communicated with the fixed sleeve, the other end of the needle tube, which is far away from the fixed sleeve, is provided with a needle head, and the needle point of the needle head has a certain width.
2. The simple continuous inoculator of a leaf vascular bundle as claimed in claim 1, wherein: the included angle between the needle head and the needle tube is 90-180 degrees.
3. The simple continuous inoculator of a leaf vascular bundle as claimed in claim 1, wherein: the fixed sleeve is internally provided with a clamping part.
4. The simple continuous inoculator of a leaf vascular bundle as claimed in claim 1, wherein: the needle head is V-shaped, and the angle of the included angle is 45-180 degrees.
5. The simple continuous inoculator of a leaf vascular bundle as claimed in claim 1, wherein: the width of the needle point is 0.2-0.5 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020442857.XU CN212413963U (en) | 2020-03-31 | 2020-03-31 | Simple continuous inoculator for blade vascular bundle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020442857.XU CN212413963U (en) | 2020-03-31 | 2020-03-31 | Simple continuous inoculator for blade vascular bundle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212413963U true CN212413963U (en) | 2021-01-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020442857.XU Active CN212413963U (en) | 2020-03-31 | 2020-03-31 | Simple continuous inoculator for blade vascular bundle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212413963U (en) |
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2020
- 2020-03-31 CN CN202020442857.XU patent/CN212413963U/en active Active
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