CN212279636U

CN212279636U - Freezing and thawing control system for human embryo freezing

Info

Publication number: CN212279636U
Application number: CN202020395683.6U
Authority: CN
Inventors: 倪晓蓓; 陈林君; 朱丽华; 孙海翔
Original assignee: Nanjing Drum Tower Hospital
Current assignee: Nanjing Drum Tower Hospital
Priority date: 2020-03-25
Filing date: 2020-03-25
Publication date: 2021-01-05
Anticipated expiration: 2030-03-25

Abstract

The utility model discloses a freezing and thawing control system for human embryo cryopreservation, which comprises a box body, wherein a computer main control system, a display system, a pipeline system and a miniature camera system are arranged in the box body, and the computer main control system controls the whole process of embryo freezing and thawing; the pipeline system comprises a liquid nitrogen delivery pipe and a plurality of pipelines, wherein one end of the liquid nitrogen delivery pipe is connected with the liquid nitrogen controller, one ends of the pipelines are connected with a refrigerating fluid storage bottle, a thawing fluid storage bottle and a waste fluid storage bottle, and the liquid nitrogen delivery pipe and the other ends of the pipelines are connected with a first needle probe; the inner wall of the box body is provided with a plurality of drawer-type units, and the drawer-type units are internally provided with miniature freezing and storing devices; the box body is internally provided with a movable track, the miniature camera system is arranged on the track and rotates along the track at regular time to shoot embryo images in the miniature freezing and storing device, and the shot embryo images are displayed through the display system. The system can be operated integrally, does not need to transfer for multiple times, and can freeze multiple embryos simultaneously.

Description

Freezing and thawing control system for human embryo freezing

Technical Field

The utility model belongs to medical equipment, concretely relates to a control system that is used for freezing of human embryo vitrification to unfreeze.

Background

The birth of the human embryo freezing and thawing technology is a milestone for the development of assisted reproduction. The australian scholars reported a message that the first example of frozen embryo transfer was pregnant since the history, since which embryo freeze thawing techniques developed rapidly, as early as 1983. In the treatment period of assisted reproduction, redundant embryos are urgently needed to be preserved, or the preservation of whole embryos is urgently needed because the development of complications after the oocyte picking operation cannot develop fresh period transplantation, the development of the embryo freezing and thawing technology solves the difficult problem of embryo preservation, is beneficial to reducing the incidence rate of multiple pregnancy and ovarian hyperstimulation syndrome (OHSS), enables each oocyte to generate higher cumulative Pregnancy Rate (PR), and generates great economic and social benefits.

At present, there are two main approaches to cryopreservation of embryos, programmed freezing technology and vitrification freezing technology. The programmed freezing technology is that embryos are placed in freezing protective agents with different concentrations step by step and then placed in a programmed cooling instrument for slow cooling, dehydration balance is performed step by step, operation is simple, but when the embryos are cooled to a temperature below the freezing point of freezing liquid, ice crystals can be generated in embryo cells so as to generate physical damage, and meanwhile, time for waiting for programmed cooling is too long. The vitrification freezing technology developed in recent decades uses a freezing protective agent with higher concentration to rapidly cool, thereby effectively avoiding the physical damage of ice crystals in the process of programmed freezing and gradually becoming a new technology of embryo freezing. However, the vitrification freezing technology has not an integrated operation system so far, the freezing process is completed manually, the requirement for technicians is high, and the time and energy cost is too high because the freezing process cannot be completed in batches. The two freezing methods need to transfer embryos for multiple times in the freezing process, so that the risk of embryo loss is increased; in addition, the two methods have the problem of cryopreservation of vectors, at present, closed vectors and open vectors are mainly used, the open vectors are rarely used clinically, the closed vectors are mainly Cryptop vectors, and the closed vectors have the problems of long operation time, high technical requirements and high price, so that the best vector in the aspects of operation, safety, cost and the like is not provided at present.

The consumable material used in the process of vitrifying and freezing the embryo is a common culture dish, freezing liquid is preheated before freezing, then the frozen liquid is sucked into the culture dish which is marked, the embryo to be frozen is transferred into different freezing liquids from a culture system step by step according to required time and then is transferred into liquid nitrogen, for the safety of a sample, the embryo of the same patient can be frozen at one time, even if the embryo of the same patient can be frozen for 2-3 times due to the time limitation, and the patient with a plurality of embryos frozen often needs to be frozen for multiple times, so the whole freezing process consumes time and labor. When unfreezing, the embryo needs to be taken out from the liquid nitrogen storage tank, balanced in unfreezing liquid with different gradients, and the reverse process of the freezing process is implemented, so that the problems of time consumption and labor consumption are also caused.

In conclusion, the existing vitrification freezing technology is lack of an integrated operating system, embryos need to be transferred for multiple times in the freezing process, the risk of embryo loss is increased, multiple embryos need to be frozen in multiple times, time and labor are consumed, and operators are easily frozen by liquid nitrogen in the operating process.

SUMMERY OF THE UTILITY MODEL

The purpose of the invention is as follows: an object of the utility model is to provide a can carry out the integration operation, need not multiple transfer and can freeze the freezing control system that unfreezes that is used for human embryo cryopreserving of many pieces of embryo simultaneously.

The technical scheme is as follows: the utility model comprises a box body, wherein a computer main control system, a display system, a pipeline system, a micro camera system, a refrigerating fluid storage bottle, a thawing fluid storage bottle and a waste fluid storage bottle are arranged in the box body, and the computer main control system controls the whole process of freezing and thawing embryos; the display system displays embryo image information and various monitoring data in the processes of freezing and unfreezing the embryo;

the pipeline system comprises a liquid nitrogen delivery pipe and a plurality of pipelines, wherein one end of the liquid nitrogen delivery pipe is connected with a liquid nitrogen controller, and the liquid nitrogen controller is externally connected with a liquid nitrogen storage tank; one ends of the plurality of pipelines are connected with a refrigerating fluid storage bottle, a thawing fluid storage bottle and a waste fluid storage bottle, and the other ends of the liquid nitrogen delivery pipes and the other ends of the plurality of pipelines are connected with corresponding first needle probes with elasticity; the liquid nitrogen controller is connected with a computer main control system, and when the balance of the refrigerating fluid is finished, the liquid nitrogen controller issues an instruction to lower a first needle type probe connected with a liquid nitrogen delivery pipe to a target position to start nitrogen injection and refrigeration;

a plurality of drawer-type units are arranged on the inner wall of the box body along the circumferential direction, and a micro freezing and storing device is arranged in each drawer-type unit; a movable track is arranged above the drawer type unit in the box body, the miniature camera system is arranged on the track, the miniature camera system moves and rotates along the track at regular time to shoot embryo images in the miniature cryopreservation device, and the shot embryo images are displayed through a display system; the micro camera system can observe the state of the embryo in the micro cryopreservation device in real time.

The micro freezing and storing device comprises a freezing pool, a connecting part and a handle, wherein the connecting part is connected between the freezing pool and the handle; a cylindrical groove is formed in the middle of the freezing tank, a wave roll is arranged at the bottom of the cylindrical groove, the cylindrical groove is divided into a washing tank part and an embryo part by the wave roll, the washing tank part is a liquid inlet and absorption part of the first needle type probe, and the embryo part is an embryo placement part; the wave roll limits the embryo in a certain space, and the embryo is prevented from being lost in the liquid discharging process of the flushing pool.

The micro freezing and storing device also comprises a protective cap, the protective cap is sleeved on the freezing tank, and the joint of the protective cap and the freezing tank is sealed; placing a drawer type basket hanging frame in the liquid nitrogen storage tank, wherein a plurality of basket boxes are arranged on the drawer type basket hanging frame, after the embryo is frozen in a cold state, the embryo is stored in the corresponding basket box after a protective cap is sleeved on a freezing pool, and placing the micro freezing and storing device into the liquid nitrogen storage tank for storage through the drawer type basket hanging frame; the protective cap is provided with a low-temperature-resistant position sensor which is used for positioning embryos in the liquid nitrogen storage tank; the protective cap avoids cross contamination of embryos in liquid nitrogen.

The computer main control system comprises a power switch, a plurality of valve controllers, a memory and a processor, wherein the valve controllers are respectively arranged on pipelines of a freezing liquid storage bottle, a thawing liquid storage bottle and a waste liquid storage bottle and a liquid nitrogen conveying pipe; the flowmeter monitors the liquid flow; the whole freezing and unfreezing process is controlled through a computer main control system, and integrated operation is realized.

The liquid nitrogen conveying pipe and the pipeline are respectively provided with a heat insulation layer, a pressure sensor, a temperature sensor and a heating system, and the heat insulation layers are used for insulating and preserving heat of the pipeline; the pressure sensor detects the pressure of the pipeline; the temperature sensor detects the temperature of the liquid in the pipeline; the heating system preheats the liquid in the pipeline; the pipeline system transmits the collected temperature, pressure and flow data to the computer main control system, and the computer main control system issues instructions according to a preset program; and automatic operation is realized.

The miniature camera system comprises a microscope and a miniature camera, and the distance of the microscope for focusing the embryo is adjusted in real time through the movement and the lifting of the track, so that the embryo image in the miniature freezing and storing device is positioned, and the image under the field of view of the microscope is shot through the miniature camera; the state of the embryo in the freezing tank is observed in real time, so that the embryo is prevented from being damaged due to over freezing.

The liquid nitrogen controller is provided with a nitrogen injection valve, a temperature sensor and an automatic pressure relief valve, and the nitrogen injection valve completes nitrogen injection according to a program instruction of the computer; the temperature sensor monitors the temperature of the pipeline; the automatic pressure relief valve maintains the pressure of the liquid nitrogen pipeline and the liquid nitrogen container within a safe range; and automatic operation is realized.

One end of each of the pipelines, which is connected with the refrigerating fluid storage bottle, the unfreezing fluid storage bottle and the waste liquid storage bottle, is provided with a second needle probe, and the second needle probes are used for sucking the liquid in the refrigerating fluid storage bottle and the unfreezing fluid storage bottle; the needle type probes which are different are convenient to flush the freezing tank and discharge waste liquid in the limited box body space.

A notch is formed in the box body corresponding to the drawer type unit, the micro freezing and storing device is installed in the drawer type unit through the notch, and a sealing assembly and a spring bolt assembly are installed at the contact part of the box body of the drawer type unit and the micro freezing and storing device; the operation is convenient.

The bottom of the drawer-type unit is funnel-shaped, a local net-shaped structure is arranged at the position, corresponding to the embryo part of the freezing tank, of the bottom of the drawer-type unit, the bottom of the drawer-type unit is connected with the top of a cylindrical groove of the freezing tank in a buckling mode, and the connection position of the drawer-type unit and the freezing tank is sealed; the partial net structure and the wave roll limit the embryos in a certain space together, and the embryos are prevented from being lost.

Has the advantages that: compared with the prior art, the utility model, its beneficial effect lies in: (1) the automation of the freezing and thawing process is quickly and effectively realized; (2) the micro cryopreservation device is an upper machine carrier in the freezing and thawing process and a cryopreservation carrier, so that the risk of embryos in the multiple transfer process is reduced; (3) the use cost of consumables is reduced, the miniature cryopreservation device is small in size and flexible in storage and taking, the space for storing embryos in the liquid nitrogen tank is saved, and meanwhile, the risk of hypoxia caused by liquid nitrogen frostbite and liquid nitrogen volatilization in the operation process of personnel is reduced; (4) the protective cap has a good sealing effect, so that cross contamination in liquid nitrogen is avoided, and the protective cap is provided with a positioning device to facilitate positioning of embryos; (5) novel structure, degree of automation height, easy operation, factor of safety height.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic sectional view taken along line A-A' of the case of the present invention;

FIG. 3 is a schematic view of the miniature cryopreservation apparatus of the present invention inserted into a drawer unit;

FIG. 4 is a schematic view of the miniature cryopreservation apparatus of the present invention inserted into the drawer unit along the B-B' section;

FIG. 5 is a schematic view of a micro cryopreservation apparatus according to the present invention;

FIG. 6 is a schematic view of the micro cryopreservation device of the present invention in a state where a protective cap is fitted over the micro cryopreservation device;

fig. 7 is a schematic view of a liquid nitrogen basket for storing a micro cryopreservation device of the present invention.

Detailed Description

The invention is described in further detail below with reference to specific embodiments and the accompanying drawings.

As shown in fig. 1, the utility model comprises a box body 1, the box body 1 comprises a computer main control system 3, a display system 4, a pipeline system, a micro camera system 6, a freezing liquid storage bottle 10, a thawing liquid storage bottle 11 and a waste liquid storage bottle 12 which are connected with each other, the pipeline system comprises a liquid nitrogen delivery pipe 7 and a plurality of pipelines 13, one end of the liquid nitrogen delivery pipe 7 is connected with a liquid nitrogen controller 8, and the liquid nitrogen controller 8 is externally connected with a liquid nitrogen storage tank; one ends of a plurality of pipelines 13 are connected with a refrigerating fluid storage bottle 10, a thawing fluid storage bottle 11 and a waste fluid storage bottle 12, the other ends of a liquid nitrogen delivery pipe 7 and the other ends of the plurality of pipelines 13 are connected with corresponding first needle probes 15 with elasticity respectively, and the first needle probes 15 are installed on the box body 1 through a support; liquid nitrogen controller 8 is connected with computer major control system 3, in this embodiment, refrigerating fluid storage bottle 10 quantity is two, the gradient of refrigerating fluid is different in two refrigerating fluid storage bottles 10, thawing solution storage bottle 11 quantity is three, the gradient of thawing solution is all inequality in three thawing solution storage bottle 11, waste liquid storage bottle 12 quantity is one, each liquid storage bottle all is furnished with independent pipeline 13, and a plurality of pipelines 13 and refrigerating fluid storage bottle 10, thawing solution storage bottle 11, the one end that waste liquid storage bottle 12 is connected all is equipped with second needle type probe 14, absorb refrigerating fluid storage bottle 10, the liquid in thawing solution storage bottle 11 through second needle type probe 14.

As shown in fig. 1, a computer main control system 3 controls the whole process of freezing and unfreezing the embryo; the computer main control system 3 comprises a power switch, a plurality of valve controllers, a memory and a processor, wherein the valve controllers are respectively arranged on pipelines 13 of a refrigerating fluid storage bottle 10, a thawing fluid storage bottle 11 and a waste fluid storage bottle 12, the valve controllers are provided with time controllers and flow meters, when a freezing and thawing program is executed, the time controllers upload an instruction of starting or ending implementation time to the processor in real time, and the processor issues the valve controller with the instruction of valve switching; the flow meter monitors the liquid flow in the pipeline 13 and in the liquid nitrogen delivery pipe 7. The memory stores a computer program that is executed by the processor.

The display system 4 displays embryo image information and various monitoring data of the embryo in the processes of freezing and unfreezing the embryo, and comprises a display and related elements, wherein the display is arranged on the side surface of the shell. The device comprises a plurality of related elements such as a time controller, a temperature sensing device, a pressure sensing device, an image acquisition card, a wireless communicator and the like, wherein the plurality of temperature sensors and the plurality of pressure sensors are respectively arranged at refrigerating fluid, unfreezing fluid and liquid nitrogen conveying pipelines, and the related elements are connected through wires sleeved with heat insulation layers. The wireless communicator uploads the temperature value, the pressure value, the time and the like to the server, and can record and store related data in the embryo freezing process in real time; the image acquisition card can transmit data to the computer main control system 3 and display the data through a display of the display system 4.

As shown in fig. 1, a liquid nitrogen delivery pipe 7 is arranged above a box body 1, one end of the liquid nitrogen delivery pipe 7 is connected with a liquid nitrogen controller 8, a nitrogen injection valve, a temperature sensor and an automatic pressure relief valve are arranged on the liquid nitrogen controller 8, and the nitrogen injection valve can complete nitrogen injection according to a program instruction of a computer; the temperature sensor detects the temperature of the pipeline; the automatic pressure relief valve maintains the pressure of the liquid nitrogen pipeline and the liquid nitrogen container within a safe range. The liquid nitrogen controller 8 is externally connected with a liquid nitrogen tank, the other end of the liquid nitrogen delivery pipe 7 is connected with a first needle type probe 15 with elasticity, and the other end of the liquid nitrogen delivery pipe 7 is also provided with a valve controller; the liquid nitrogen controller 8 is connected with the computer main control system 3, and when the balance of the refrigerating fluid is finished, the liquid nitrogen controller 8 issues an instruction to lower a first needle probe 15 connected with a liquid nitrogen delivery pipe 7 to a target position to start nitrogen injection and refrigeration; the first needle probe 15 has the same structure as the second needle probe 14, but the first needle probe 15 connected to the liquid nitrogen delivery pipe 7 is resistant to low temperature.

The liquid nitrogen delivery pipe 7 and the pipeline 13 are respectively provided with a heat insulation layer, a pressure sensor, a temperature sensor and a heating system, and the heat insulation layers are used for insulating and preserving heat of the pipeline; the pressure sensor detects the pressure of the pipeline; the temperature sensor detects the temperature of the liquid in the pipeline; the heating system preheats the liquid in the pipeline; the pipeline system transmits the collected temperature, pressure and flow data to the computer main control system 3 for recording and storing, and the computer main control system 3 issues instructions according to a preset program.

As shown in fig. 2 to 6, a plurality of drawer units 2 are uniformly arranged on the inner wall of the box body 1 along the circumferential direction, and a micro cryopreservation device 5 is arranged in each drawer unit 2; a notch is formed in the box body 1 at a position corresponding to the drawer type unit 2, and the micro freezing and storing device 5 is arranged in the drawer type unit 2 through the notch; the contact part of the drawer type unit box body and the miniature preservation device 5 is provided with a sealing component and a spring bolt component. The micro freezing and storing device 5 comprises a freezing tank 21, a connecting part 22, a handle 23 and a protective cap 24, wherein the connecting part 22 is connected between the freezing tank 21 and the handle 23; a cylinder groove 20 is formed in the middle of the freezing tank 21, a wave roll 19 is arranged at the bottom of the cylinder groove 20, the wave roll 19 divides the cylinder groove 20 into a washing tank portion 17 and an embryo portion 18, the washing tank portion 17 is a liquid inlet and liquid absorption portion of the first needle type probe 15, and the embryo portion 18 is an embryo placement portion. The handle 23 can be pasted with a two-dimensional code electronic tag printed with embryo information and color, and the electronic tag uploads the information to a cloud server after scanning to generate a human embryo freezing and thawing database. The protective cap 24 is sleeved on the freezing tank 21, and the joint of the protective cap 24 and the freezing tank 21 is sealed, so that the risk of mutual cross contamination of embryos in liquid nitrogen can be avoided; as shown in fig. 7, a drawer type basket hanging rack 25 is placed in the liquid nitrogen tank, a plurality of basket boxes 26 are arranged on the drawer type basket hanging rack 25, after the embryo is frozen, the embryo is stored in the corresponding basket box 26 after the freezing tank 21 is sleeved with a protective cap 24, and the micro freezing storage device 5 is placed in the liquid nitrogen tank for storage through the drawer type basket hanging rack 25, so that a large amount of storage space is saved; the protective cap 24 is provided with a low temperature resistant position sensor which locates the embryo in the liquid nitrogen tank.

As shown in fig. 4, the bottom of the drawer-type unit 2 is funnel-shaped, a partial net-shaped structure 16 is disposed at the bottom of the drawer-type unit 2 corresponding to the embryo portion 18 of the freezing tank, the bottom of the drawer-type unit 2 is connected with the top of the cylindrical groove 20 of the freezing tank 21 by a snap-fit, and the connection between the drawer-type unit 2 and the freezing tank 21 is sealed. The partial network 16 houses a water level sensor for sensing the level of liquid in the cylindrical recess 20.

Because the embryos need to be in a liquid environment all the time, the wave roll 19 at the bottom of the cylindrical groove 20 in the freezing tank 21 is of great importance, the wave roll 19 can always ensure the water level of the embryos, and the embryos are locked in a certain space with the partial reticular structure 16 right above the wave roll, so that the embryos are prevented from being lost in the liquid discharging process of the washing tank. The material of the micro-storage device 5 is low temperature resistant, the bottom is transparent, the bottom is flat, and a technician can transfer embryos under a microscope.

As shown in FIG. 1, a movable rail 9 is arranged above the drawer-type unit 2 in the box 1, the micro camera system 6 is arranged on the rail 9, and the micro camera system 6 is periodically moved and rotated along the rail 9 to shoot the embryo image in the micro cryopreservation device 5, and the shot embryo image is displayed by the display system 4. The micro camera system 6 comprises a microscope and a micro camera, the distance of the microscope for focusing the embryo is adjusted in real time through the movement and the lifting of the track 9, so that the embryo image in the micro freezing and storing device 5 is positioned, and the image under the microscope field is shot through the micro camera.

When the liquid inlet program is executed, the first needle probe 15 of the target liquid pipeline moves along the track and stretches to the target position, liquid is fed to the pool flushing part 17 of the freezing pool, and when the liquid reaches the sample inlet volume, a signal is transmitted to the controller to close the liquid inlet valve and lift the liquid inlet valve to the box body part. When the liquid outlet program is executed, a first needle probe 15 on a pipeline 13 connected with the waste liquid storage bottle 12 moves along a track and stretches to a target position, the first needle probe is opposite to a pool flushing part 17 of the freezing pool to start liquid suction, and when the liquid outlet volume reaches a specified value, a signal can be transmitted to the controller to close the liquid outlet valve and lift the liquid outlet valve to the box body part.

The specific operation process is as follows:

1. freezing process

1) Preparing a portable freezing pool: before freezing, firstly, the handle 23 of the micro freezing storage device 5 is pasted with low temperature resistant electronic labels with different colors, the labels are marked with patient information such as name, medical record number, embryo period and the like, the labels can be printed and can be scanned into a server to generate a human embryo freezing database. Adding balanced culture solution into the embryo part 18 in the cylindrical groove 20 of the freezing tank 21, sucking the corresponding embryo into the embryo part 18 of the freezing tank 21 under a microscope after double checking, putting the freezing tank 21 filled with the embryo into an incubator for transfer, and finishing the embryo sample loading after the embryos needing to be mechanically frozen together;

2) freezing and loading on a machine: firstly, preparing, turning on a power supply, filling a refrigerating fluid storage bottle 10 and a waste fluid storage bottle 12 into a liquid nitrogen storage tank, cleaning a pipeline system, and inserting a prepared micro freezing and storing device 5 into a drawer type unit 2 for freezing; the freezing procedure is started, the first needle type probe 15 on the pipeline 13 of the first freezing liquid storage bottle moves to stretch to the target position, the first needle type probe is opposite to the pool flushing part 17 of the freezing pool 21, the valve controller on the first freezing liquid storage bottle is opened, liquid feeding is started, when liquid reaches the sampling volume, the valve controller on the first freezing liquid storage bottle is closed by a transmission signal to the controller, and the first needle type probe 15 corresponding to the first freezing liquid storage bottle is lifted to the box body part. The embryo is balanced a period in first refrigerating fluid, and the first needle probe 15 on the pipeline 13 of back waste liquid storage bottle moves flexible to the target position after the procedure preset time arrives, just faces towards pond portion 17 of freezing pond 21, and the drain valve is opened, begins to go out the liquid, and first refrigerating fluid flows into waste liquid storage bottle 12, but liquid valve is closed to the controller to the transferable signal when going out the liquid volume and reaching regulation numerical value, and the first needle probe 15 that waste liquid storage bottle 12 corresponds lifts to the box portion. First needle probe 15 on the pipeline 13 of second cryogenic fluid storage bottle removes to stretch out and draw back to the target location, just to the portion 17 of dashing of freezing pond 21, and the second cryogenic fluid inlet valve is opened, and the second cryogenic fluid dashes the pond, and transmission signal closes the valve controller on the second cryogenic fluid storage bottle to the controller when liquid reachs the appearance volume, and first needle probe 15 that the second cryogenic fluid storage bottle corresponds lifts to box portion. After the preset time is reached, the first needle probe 15 on the pipeline 13 of the waste liquid storage bottle moves and stretches to a target position, the first needle probe is opposite to the pool flushing part 17 of the freezing pool 21, the liquid outlet valve is opened to begin to discharge liquid, the second freezing liquid flows into the waste liquid storage bottle 12, when the liquid outlet volume reaches a specified value, a signal can be transmitted to the controller to close the liquid outlet valve, and the first needle probe 15 corresponding to the waste liquid storage bottle is lifted to the box body part. Meanwhile, the first needle probe 15 corresponding to the liquid nitrogen delivery pipe 7 moves and stretches to a target position, the first needle probe is opposite to the tank flushing part 17 of the freezing tank 21, a nitrogen injection valve is opened, the liquid nitrogen flushes the tank, when the volume of the discharged nitrogen reaches a specified value, a signal can be transmitted to a liquid nitrogen controller to close the nitrogen outlet valve, the first needle probe 15 corresponding to the liquid nitrogen delivery pipe 7 is lifted to the tank body part, and the embryo is frozen; in the process, the state of each step of the embryo can be shot by a microscope and a miniature camera in the box body 1 and displayed on a display of the display system 4;

3) sealing the freezing pool: the handle 23 is held by hand, the micro freezing and storing device 5 is taken out of the drawer type unit 2, the protective cap 24 is sleeved on the micro freezing and storing device, the micro freezing and storing device 5 is placed into liquid nitrogen to be turned into a box, and all the caps of the micro freezing and storing device 5 to be frozen together are sleeved;

4) and (3) liquid nitrogen preservation: the micro cryopreservation device 5 sleeved with the protective cap 24 is put into a liquid nitrogen basket box 26 and put into a liquid nitrogen storage tank for storage.

2. Thawing process

1) Preparing: before thawing, firstly searching the cryopreservation position and color of a patient to be thawed according to an operation notice and the freezing information, quickly positioning according to an electronic positioning system provided by a protective cap 24, taking out the patient from a liquid nitrogen storage tank, checking the patient by two persons, putting the patient into a liquid nitrogen transfer box, taking all the embryos to be thawed in the same batch out, putting the embryos into the liquid nitrogen transfer box, and preparing for thawing and loading the embryos on a computer;

2) unfreezing and processing on a machine: turning on a power supply, filling the unfreezing liquid storage bottle 11 and the waste liquid storage bottle 12, cleaning a pipeline system, starting a liquid preheating program in the unfreezing process before a sample is loaded on a machine, and preheating the unfreezing liquid storage bottle and a corresponding pipeline; inserting the prepared micro cryopreservation device 5 into the drawer unit 2 to prepare for thawing; first needle probe 15 on the pipeline 13 of first thawing liquid storage bottle removes flexible to the target location, just to freezing pool 21 towards pool portion 17, and first thawing liquid feed liquor valve is opened, and transmission signal closes first thawing liquid feed liquor valve to the controller when liquid reachs the appearance volume, and first needle probe 15 that first thawing liquid storage bottle corresponds lifts to box portion. The embryo is balanced a period in first thawing liquid, and after the program preset time arrives, first needle probe 15 on pipeline 13 of waste liquid storage bottle 12 moves and stretches to the target position, just faces towards pond portion 17 of freezing pond 21, and the liquid outlet valve opens, begins to go out liquid, and first thawing liquid flows into waste liquid storage bottle 12, but liquid outlet valve is closed to the controller to the transmissible signal when liquid volume reaches the regulation numerical value, and first needle probe 15 that waste liquid storage bottle corresponds lifts to the box portion. The first needle probe 15 on the pipeline 13 of the second thawing liquid storage bottle moves and stretches to a target position, the first needle probe is opposite to the pool flushing part 17 of the freezing pool 21, the second thawing liquid inlet valve is opened, the second thawing liquid flushing pool is used for transmitting a signal to the controller to close the second thawing liquid inlet valve when the liquid reaches the sampling volume, and the first needle probe 15 corresponding to the second thawing liquid storage bottle is lifted to the box body part. After the preset time is reached, the first needle probe 15 on the pipeline 13 of the waste liquid storage bottle moves and stretches to a target position, the first needle probe is opposite to the pool flushing portion 17 of the freezing pool 21, the liquid outlet valve is opened to begin to discharge liquid, the second unfreezing liquid flows into the waste liquid storage bottle 12, when the liquid outlet volume reaches a specified value, a signal can be transmitted to the controller to close the liquid outlet valve, and the first needle probe 15 corresponding to the waste liquid storage bottle is lifted to the box portion. The first needle probe 15 on the pipeline 13 of the third thawing liquid storage bottle moves and stretches to the target position, and is opposite to the pool flushing part 17 of the freezing pool 21, the third thawing liquid inlet valve is opened, the third thawing liquid flushing pool is opened, when the liquid reaches the sample injection volume, a signal is transmitted to the controller to close the third thawing liquid inlet valve, and the first needle probe 15 corresponding to the third thawing liquid storage bottle is lifted to the box body part. After the preset time is reached, the first needle probe 15 on the pipeline 13 of the waste liquid storage bottle moves and stretches to a target position, the first needle probe is opposite to the pool flushing part 17 of the freezing pool 21, the liquid outlet valve is opened to begin to discharge liquid, the third unfreezing liquid flows into the waste liquid storage bottle 12, when the liquid outlet volume reaches a specified value, a signal can be transmitted to the controller to close the liquid outlet valve, and the first needle probe 15 corresponding to the waste liquid storage bottle is lifted to the box body part. After thawing, the cryopreservation apparatus 5 is taken out of the drawer unit 2 and put into an incubator for use.

Claims

1. A freeze-thaw control system for human embryo cryopreservation, comprising a box body (1), characterized in that: a computer main control system (3), a display system (4), a pipeline system, a miniature camera system (6), a refrigerating fluid storage bottle (10), a thawing fluid storage bottle (11) and a waste fluid storage bottle (12) are arranged in the box body (1), and the computer main control system (3) controls the whole process of freezing and thawing of embryos; the display system (4) displays embryo image information and various monitoring data in the processes of freezing and unfreezing the embryo;

the pipeline system comprises a liquid nitrogen delivery pipe (7) and a plurality of pipelines (13), one end of the liquid nitrogen delivery pipe (7) is connected with a liquid nitrogen controller (8), and the liquid nitrogen controller (8) is externally connected with a liquid nitrogen storage tank; one ends of a plurality of pipelines (13) are connected with a refrigerating fluid storage bottle (10), a thawing fluid storage bottle (11) and a waste fluid storage bottle (12), and the other ends of a liquid nitrogen delivery pipe (7) and the other ends of the plurality of pipelines (13) are connected with corresponding first needle probes (15) with elasticity; the liquid nitrogen controller (8) is connected with the computer main control system (3), and when the balance of the refrigerating fluid is finished, the liquid nitrogen controller (8) issues an instruction to lower a first needle probe (15) connected with the liquid nitrogen delivery pipe (7) to a target position to start nitrogen injection and refrigeration;

a plurality of drawer-type units (2) are arranged on the inner wall of the box body (1) along the circumferential direction, and a micro freezing and storing device (5) is arranged in each drawer-type unit (2); a movable track (9) is arranged above the drawer type unit (2) in the box body (1), the miniature camera system (6) is arranged on the track (9), the miniature camera system (6) moves and rotates along the track (9) at regular time to shoot embryo images in the miniature freezing and storing device (5), and the shot embryo images are displayed through the display system (4).

2. The freeze-thaw control system for human embryo cryopreservation according to claim 1, wherein: the micro freezing and storing device (5) comprises a freezing pool (21), a connecting part (22) and a handle (23), wherein the connecting part (22) is connected between the freezing pool (21) and the handle (23); a cylinder groove (20) is formed in the middle of the freezing tank (21), a wave roll (19) is arranged at the bottom of the cylinder groove (20), the wave roll (19) divides the cylinder groove (20) into a washing tank part (17) and an embryo part (18), the washing tank part (17) is a liquid inlet and liquid absorption part of the first needle type probe (15), and the embryo part (18) is an embryo placement part.

3. The freeze-thaw control system for human embryo cryopreservation according to claim 2, wherein: the micro freezing and storing device (5) further comprises a protective cap (24), the protective cap (24) is sleeved on the freezing tank (21), and the connection part of the protective cap (24) and the freezing tank (21) is sealed; a drawer type basket hanging rack (25) is placed in the liquid nitrogen storage tank, a plurality of basket boxes (26) are arranged on the drawer type basket hanging rack (25), after the embryos are frozen in a cold mode, the embryos are stored in the corresponding basket boxes (26) after being sleeved with a protective cap (24) by a freezing pool (21), and the micro freezing and storing device (5) is placed in the liquid nitrogen storage tank for storage through the drawer type basket hanging rack (25); the protective cap (24) is provided with a low temperature resistant position sensor which positions embryos in the liquid nitrogen tank.

4. The freeze-thaw control system for human embryo cryopreservation according to claim 1, wherein: the computer main control system (3) comprises a power switch, a plurality of valve controllers, a memory and a processor, wherein the valve controllers are respectively arranged on a pipeline (13) of a freezing liquid storage bottle (10), a thawing liquid storage bottle (11) and a waste liquid storage bottle (12) and a liquid nitrogen conveying pipe (7), a time controller and a flowmeter are arranged on the valve controllers, when freezing and thawing procedures are executed, the time controller uploads an instruction of starting or ending implementation time to the processor in real time, and the processor issues the valve controller with the instruction of valve switching; the flow meter monitors the liquid flow.

5. The freeze-thaw control system for human embryo cryopreservation according to claim 4, wherein: the liquid nitrogen conveying pipe (7) and the pipeline (13) are respectively provided with a heat insulation layer, a pressure sensor, a temperature sensor and a heating system, and the heat insulation layers are used for insulating and preserving heat of the pipeline; the pressure sensor detects the pressure of the pipeline; the temperature sensor detects the temperature of the liquid in the pipeline; the heating system preheats the liquid in the pipeline; the pipeline system transmits the collected temperature, pressure and flow data to the computer main control system (3), and the computer main control system (3) issues instructions according to a preset program.

6. The freeze-thaw control system for human embryo cryopreservation according to claim 1, wherein: the micro camera system (6) comprises a microscope and a micro camera, the distance of the microscope for focusing the embryo is adjusted in real time through the movement and the lifting of the track (9), so that the embryo image in the micro freezing and storing device (5) is positioned, and the image under the microscope field is shot through the micro camera.

7. The freeze-thaw control system for human embryo cryopreservation according to claim 1, wherein: the liquid nitrogen controller (8) is provided with a nitrogen injection valve, a temperature sensor and an automatic pressure relief valve, and the nitrogen injection valve completes nitrogen injection according to a program instruction of a computer; the temperature sensor monitors the temperature of the pipeline; the automatic pressure relief valve maintains the pressure of the liquid nitrogen pipeline and the liquid nitrogen container within a safe range.

8. The freeze-thaw control system for human embryo cryopreservation according to claim 4, wherein: one end of each of the plurality of pipelines (13) connected with the refrigerating fluid storage bottle (10), the unfreezing fluid storage bottle (11) and the waste fluid storage bottle (12) is provided with a second needle probe (14), and liquid in the refrigerating fluid storage bottle (10) and the unfreezing fluid storage bottle (11) is sucked through the second needle probe (14).

9. The freeze-thaw control system for human embryo cryopreservation according to claim 1, wherein: a notch is formed in the box body (1) at a position corresponding to the drawer type unit (2), and the micro freezing and storing device (5) is arranged in the drawer type unit (2) through the notch; the contact part of the drawer type unit box body and the micro freezing preservation device (5) is provided with a sealing component and a spring bolt component.

10. The freeze-thaw control system for human embryo cryopreservation according to claim 2, wherein: the bottom of the drawer-type unit (2) is funnel-shaped, a local net-shaped structure (16) is arranged at the position, corresponding to the embryo part (18) of the freezing tank, of the bottom of the drawer-type unit (2), the bottom of the drawer-type unit (2) is connected with the top of a cylindrical groove (20) of the freezing tank (21) in a buckling mode, and the connection position of the drawer-type unit (2) and the freezing tank (21) is sealed.