CN212255220U - Automatic reagent sampling thin-layer chromatography device - Google Patents

Abstract

The utility model discloses an automatic reagent sampling thin layer chromatography device, including the capillary frame that is used for installing the capillary, X axial actuating mechanism, Y axial drive structure and test bench, X axial actuating mechanism and Y axial actuating mechanism are used for driving the capillary frame respectively and remove in X axle and Y axle direction, the capillary frame sets up in the test bench top, be equipped with the reagent test tube seat of placing the test tube on the test bench, be used for placing the layer board of thin layer chromatography board and be located the exhibition jar of layer board one side bottom, still be provided with rotary mechanism and the moving mechanism who drives the layer board rotation respectively and remove on the test bench. The utility model has simple and reasonable structure, convenient disassembly and cleaning and intelligent operation; the device of the utility model can effectively reduce the variables influencing the experimental result and reduce the experimental error; use the utility model discloses a consumption of thin layer chromatography board can also effectively be saved to the device, practices thrift the experiment cost, avoids the wasting of resources.

Description

Automatic reagent sampling thin-layer chromatography device

Technical Field

The utility model relates to a chemical industry equipment technical field, concretely relates to automatic reagent sampling thin layer chromatography device.

Background

The thin layer chromatography is one of chromatography, and utilizes the difference of physical properties of each component to separate and measure a multi-component mixture, and is mainly used for quickly separating and qualitatively analyzing a small amount of substances and separating a small amount of samples in a laboratory. The detection method needs to prepare a thin-layer chromatography plate, the thin-layer chromatography plate is a plane plate with the surface uniformly coated with an adsorbent, a carrier or other active substances, and the bottom plate can be made of glass plates, plastic sheets, metal sheets and the like. At present, a beaker is generally used for containing a developing agent, then a thin layer chromatography plate is placed in the beaker containing the developing agent for detection, and simultaneously, an ultraviolet lamp is used for irradiating the thin layer chromatography plate for observation and analysis.

In the laboratory of colleges and universities and enterprises, this process is all accomplished by the laboratory technician usually, and the laboratory technician all need spend a large amount of time every day and carry out the chromatography experiment, has greatly restricted work efficiency, and the laboratory technician can't guarantee in the chromatography experiment that the angle, the immersion depth, the immersion time of some board dynamics, some board time, thin layer chromatography board immersion developing agent keep unanimous, leads to the experiment to have extra variable influence experimental data.

In conclusion, a machine can be used for replacing simple and repeated manual labor, the labor input in the chromatography experiment is reduced, the machine replaces the manual labor to complete the task, the experiment efficiency is improved, the point plate strength, the immersion angle and the millisecond precision are controlled strictly through software delay and program setting, the point plate immersion time and the chromatography plate immersion developer time are controlled strictly, and the experiment error is reduced.

At present, similar automatic plate counting devices and thin-layer chromatography devices have few patents, and a few devices related to the experiment have simple structure and single function and have various defects, for example, a thin-layer chromatography experimental device with the application number of CN201720251303.X only sets up an observation platform after sampling is completed, and the specific experimental operation steps are not simplified; in application No. CN201721860102.6, a device integrating thin layer chromatography spotting and development is designed, but the device has a simple and crude structure, low working efficiency and no function of automatically dipping a developing agent. The utility model discloses to above not enough, solve above device bulky, the function singleness, change and wash shortcoming inconvenient, that degree of automation is low.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an automatic reagent sampling thin layer chromatography device of degree of automation height, change convenience, labour saving and time saving, reducible experimental error.

The utility model provides an automatic reagent sampling thin layer chromatography device, is including capillary frame, actuating mechanism and the test bench that is used for installing the capillary, actuating mechanism is used for driving the capillary frame removes, the capillary frame sets up the test bench top, be equipped with the reagent test tube seat of placing the test tube on the test bench, be used for placing the layer board of thin layer chromatography board and be located the exhibition jar of layer board one side bottom, still be provided with the drive respectively on the test bench rotatory and moving mechanism who removes of layer board.

Further, the driving mechanism comprises an X-axis driving mechanism and a Z-axis driving mechanism, the X-axis driving mechanism comprises a first seat body, a first stepping motor and a first sliding table, the first sliding table is arranged on the first seat body in a sliding manner along the X-axis direction through a lead screw nut mechanism, the first stepping motor is used for driving the lead screw nut mechanism, the Z-axis driving mechanism comprises a second seat body, a second stepping motor and a second sliding table, the second seat body is arranged on the first sliding table, the second sliding table is arranged on the second seat body in a sliding manner along the Z-axis direction through the lead screw nut mechanism, the second stepping motor is used for driving the lead screw nut mechanism, the capillary tube frame is arranged on the second sliding table, the X-axis driving mechanism can drive the first sliding table to move in the X-axis direction, namely, the capillary tube frame can move in the X-axis direction, the Z-axis driving mechanism can drive the second sliding table to move in the Z-axis direction, namely the capillary tube frame can move in the Z-axis direction, and the capillary tube frame is connected with a capillary tube; the capillary tube can finish the function of automatically sampling from a test bench and then dotting the plate by moving in the X-axis direction and the Z-axis direction, the capillary tube rack is moved to the upper part of a reagent test tube seat by a specific driving mechanism for controlling the X-axis direction, then the capillary tube is moved downwards into a test tube for storing a sample by controlling the driving mechanism for controlling the Z-axis direction, the sample in the test tube is absorbed by the capillary effect of the capillary tube, then the capillary tube is moved upwards by controlling the driving mechanism for controlling the Z-axis direction to be separated from the sample test tube, then the capillary tube is moved to the upper position of a supporting plate along the X-axis direction by controlling the capillary tube to move downwards by controlling the driving mechanism for controlling the capillary tube to be contacted with the thin-layer chromatography plate, the thin-layer chromatography plate placed on the supporting plate is spotted, and the force and the time of the spotting plate are accurately controllable by the matching, the error of an artificial point plate is avoided, the experimental efficiency is improved, the error is reduced, the second seat body is arranged on the first sliding table through the sliding table connecting piece, a screw hole is formed in the specific sliding table connecting piece, a screw hole corresponding to the screw hole is formed in the second seat body, the sliding table connecting piece is fixedly connected with the second seat body through the matching of the bolt and the screw hole, the same screw hole is also formed in the first sliding table, and the sliding table connecting piece is fixedly connected with the first sliding table through the matching of the bolt and the screw hole, namely, the connection between the second seat body and the first sliding table is realized, the design enables the second seat body to be conveniently arranged on the first sliding table, and the second seat body (the second sliding table) to have the free movement in the X-axis; in addition, the capillary tube mounting frame is fixedly connected to the second sliding table through bolts, namely, one end of the capillary tube frame is provided with a threaded connecting hole, the corresponding position of the second sliding table is also provided with a threaded connecting hole, and the capillary tube frame is mounted on the second sliding table by screwing bolts into the threaded connecting holes.

Further, a second concave groove is arranged in the middle of the second sliding table, a second mounting groove is arranged on the second seat body, a second stepping motor is fixedly connected in the mounting groove and positioned in the second concave groove, a second support plate for connecting a second guide rod is further arranged on the second seat body, the second support plate is fixedly connected at two ends of the second seat body, the second sliding table is slidably connected on the second guide rod, a first concave groove is arranged in the middle of the first sliding table, a first mounting groove is arranged on the first seat body, the first stepping motor is fixedly connected in the first mounting groove and positioned in the first concave groove, a first support plate for connecting the first guide rod is further arranged on the first seat body, the first support plate is fixedly connected at two ends of the first seat body, the first sliding table is slidably connected on the first guide rod, a second concave groove is formed in the second sliding table, a second mounting hole is formed in the second base body, and the second stepping motor is fixed in the second mounting groove and located in the second concave groove; in addition, the second support plate and the second guide rod are designed to limit the moving direction of the second sliding table, so that the second sliding table only moves up and down along the Z-axis direction, and the capillary can be vertically inserted into a sample test tube for sampling, thereby ensuring the success of sampling; the purpose of designing first concave groove and first mounting groove is the same with second concave groove and second mounting groove, and the difference lies in having increased the ascending portable distance of first slip table (second pedestal promptly) X axle direction, designs first backup pad and first guide bar and is for spacing first slip table, makes first slip table (second pedestal) only remove along X axle direction.

Further, a plurality of mounting holes are formed in the capillary tube support along the length direction of the capillary tube support, the distance between the axes of every two adjacent mounting holes is 10mm, a clamping device is arranged in each mounting hole, a capillary tube hole used for mounting a capillary tube is formed in each clamping device, each clamping device comprises a threaded connecting portion and a clamping portion located at the bottom of each threaded connecting portion, one end of each threaded connecting portion penetrates through each mounting hole and is connected with an upper fastening nut, the other end of each threaded connecting portion is connected with a lower fastening nut, each clamping portion is of a circular table type structure formed by surrounding a plurality of clamping blocks, a fastening sleeve used for fastening the clamping portions is further sleeved between each lower fastening nut and the clamping portion, the inner diameter of each fastening sleeve is larger than the diameter of the upper end of each clamping portion and smaller than the diameter of the lower end of each clamping portion, the capillary tube is convenient to replace, and the whole clamping device is, when the capillary tube needs to be replaced, the lower fastening nut is rotated to enable the lower fastening nut not to extrude the fastening sleeve, the clamping portion is not extruded by the fastening sleeve, the capillary tube can be drawn out after the capillary tube is replaced, the lower fastening nut is rotated to extrude the fastening sleeve, the fastening sleeve is close to the clamping portion, the inner diameter of the fastening sleeve is larger than the diameter of the upper end of the clamping portion and smaller than the diameter of the lower end of the clamping portion, so that the fastening sleeve can have fastening force on the side surface of the circular truncated cone type clamping portion, the clamping portion clamps the capillary tube to complete replacement, and the capillary tube is fixed.

Further, a plurality of round holes for placing test tubes are arranged on the reagent test tube seat along the length direction of the reagent test tube seat, the distance between the axes of the two adjacent round holes is 10mm, the reagent test tube seat is movably arranged on the test bed, a cleaning test tube seat is also movably arranged on the test bed and is arranged in parallel to the reagent test tube seat, a plurality of round holes for placing test tubes are arranged on the cleaning test tube seat along the length direction of the cleaning test tube seat, the distance between the axes of the two adjacent round holes is 10mm, and a sponge layer is also arranged on the test bed between the reagent test tube seat and the cleaning test tube seat; the reagent test tube seat and the cleaning test tube seat are movably arranged, so that the sample reagent and the cleaning solution can be conveniently replaced, the axial distance between adjacent round holes on the reagent test tube seat and the cleaning test tube seat is designed to be 10mm, the reagent test tube seat and the cleaning test tube seat are adapted to a commercially available 'bullet' test tube, and the reagent test tube seat and the cleaning test tube seat are designed according to the diameter of the 'bullet' test tube; in addition, reagent test tube seat and the mode that washs the test tube seat activity and set up on the test bench are many, both ends of reagent test tube seat and washing test tube seat in this application all are provided with the side arm, be provided with the draw-in groove on the test bench, connect reagent test tube seat and washing test tube seat on the test bench in the draw-in groove of side arm embedding test bench, still design the sponge layer on the test bench, the purpose of this design is to carrying out the drying to the capillary after wasing, it is specific for after the capillary absorbs the washing liquid from wasing the test tube, remove the capillary to the sea level layer, absorb away through the washing liquid in with the capillary, then the capillary is removing and is absorbing the sample, thereby reached the abluent mesh to the capillary, promote the experimental effect.

Further, the below of test bench is equipped with the bottom plate, the test bench with the bottom plate passes through a plurality of bracing piece fixed connection, all be provided with the screw thread at the both ends of bracing piece, also be provided with the threaded connection hole at the edge of test bench, the one end of bracing piece and the threaded connection hole fixed connection at test bench edge, also be equipped with the threaded connection hole on the edge of bottom plate, the other end of bracing piece and the threaded connection hole fixed connection on the bottom plate, link into an integrated entity bottom plate and test bench through a plurality of bracing pieces, can fixed drive layer board pivoted slewing mechanism and the moving mechanism that the drive layer board removed along the Y axle direction on the bottom plate to and place the exhibition jar on the bottom plate, drive the thin layer chromatography version of connection on the layer board after the layer board rotates and dip the.

Furthermore, a rotating mechanism for driving the support plate to rotate is fixedly arranged on the bottom plate, one end of a rocker is connected to a rotating part of the rotating mechanism, the other end of the rocker is rotatably connected with a T-shaped sliding block, the T-shaped sliding block is slidably connected in a support plate sliding rail and has two moving degrees of freedom in the X-axis direction and the Y-axis direction, the support plate sliding rail is arranged at the bottom of the support plate, the expanding cylinder is arranged on the bottom plate, the support plate sliding rail is arranged along the X-axis direction, so that the T-shaped sliding block is slidably connected in the support plate sliding rail and can move in the X-axis direction, in addition, the width of the support plate sliding rail along the Y-axis direction is more than 5mm (namely the movable distance of the support plate in the Y-axis direction is 5mm) of the T-shaped sliding block, the T-shaped sliding block can move in the Y-axis direction, the design enables the T-shaped sliding block to move in the X-axis direction to drive the supporting plate to rotate, and enables the T-shaped sliding block to move in the Y-axis direction to drive the supporting plate to move in the Y-axis direction.

Furthermore, a moving mechanism for driving the supporting plate to move along the length direction of the reagent test tube seat is fixedly arranged on the bottom plate, the rotating part of the moving mechanism is provided with an external gear which is meshed with the rack, the supporting plate is rotationally arranged on the test bed through a rotating shaft, the rack is rotationally connected to the rotating shaft through a connecting rod, a support plate at the joint of the connecting rod and the rotating shaft is provided with a yielding groove, the yielding groove limits the connecting rod in the axial direction of the rotating shaft (namely the connecting rod can only rotate in the yielding groove but can not move along the Y-axis direction), a through hole is arranged on the test bench, one end of the rotating shaft is fixedly connected on the supporting plate, the other end of the rotating shaft passes through the through hole and is rotatably connected on the test bench, and the rotating shaft has freedom of movement in the Y-axis direction in the through hole, and the supporting plate can move in the Y-axis direction through the driving of the moving mechanism.

Further, still the activity is provided with on the layer board and is used for fixing the splint of thin layer chromatography board, and splint slip sets up the tip at the layer board, is provided with the screw on splint and layer board, and the accessible bolt is connected the splint at the tip of layer board, can make thin layer chromatography board fix on the layer board through splint when the layer board rotates, conveniently immerses the development agent in the exhibition jar with thin layer chromatography board.

The utility model has the advantages that:

the utility model has simple and reasonable structure, convenient disassembly and cleaning, intelligent operation, automatic completion of capillary cleaning, reagent sampling, plate spotting and reagent sampling by immersing into the developing agent, development of the reagent in the process of greatly reducing repeated labor in the experimental process, effective reduction of variables influencing the experimental result, reduction of experimental errors and high practicability;

the utility model discloses increased the actuating mechanism of Y axle direction, made thin layer chromatography board layer board can move in Y axle direction, after accomplishing a point board, operating personnel can select to change all by the sampling reagent, drive thin layer chromatography board layer board and remove along the Y axle, carry out the point board procedure once more (when carrying out the analysis of sampling point board on thin layer chromatography board, every sampling point generally only need apart from 5mm can, the utility model relates to an interval between the capillary is 10mm to and can move the distance in the design Y axle direction and be 5mm, can carry out the point board once more after the drive layer board removes 5mm along the Y axle, can effectively save thin layer chromatography board consumption, practice thrift the experiment cost, avoid the wasting of resources.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of the structure of the clamping device of the present invention;

FIG. 3 is a schematic diagram of the mechanism of the middle support plate of the present invention;

fig. 4 is a schematic view of the installation of the capillary frame, the holding device and the capillary tube according to the present invention;

fig. 5 is an exploded view of the present invention;

fig. 6 is a flowchart of the execution procedure of the present invention.

In the figure: 1 first sliding table, 2 first seat body, 3 first guide rod, 4 first supporting plate, 5 first stepping motor, 6 second sliding table, 7 second seat body, 8 second guide rod, 9 second supporting plate, 10 second stepping motor, 11 rotating mechanism, 12 moving mechanism, 13 extending cylinder, 14 rocker, 15 external gear, 16 rack, 17 bottom plate, 18 supporting plate, 19 supporting rod, 20 supporting plate slide rail, 21 test bed, 22 reagent test tube seat, 23 sponge layer, 24 cleaning test tube seat, 25 capillary tube rack, 26 capillary tube, 27 clamping device, 28 threaded connection part, 29 clamping part, 30 clamping block, 31 upper clamping nut, 32 lower clamping nut, 33 clamping sleeve, 34 rotating shaft, 35 abdicating groove, 36 clamping plate, 37T-shaped sliding block, 38 sliding table connection part, 39 connecting rod, 40 thin layer chromatography plate.

Detailed Description

The present invention will be described in detail with reference to the following embodiments:

examples

As shown in fig. 1, an automatic reagent sampling thin-layer chromatography device comprises a capillary tube rack 25, an X-axis driving structure, a Z-axis driving mechanism, a test bed frame 21, a reagent test tube seat 22, a cleaning test tube seat 24, a sponge layer 23, a developing cylinder 13, a supporting plate 18, a rotating mechanism 11 and a moving mechanism 12; as shown in fig. 4, the capillary tube frame 25 is provided with a plurality of mounting holes with an axial distance of 10mm along the length direction thereof, the mounting holes are connected with a clamping device 27, a capillary hole for mounting the capillary tube 26 is arranged in the clamping device 27, and the diameter of the capillary hole is slightly larger than that of the capillary tube; as shown in fig. 2, the clamping device 27 comprises a threaded connection portion 28 and a clamping portion 29 (the clamping portion is surrounded by 4 clamping blocks 30 to form a truncated cone-shaped structure), the clamping portion 29 is located at the bottom of the threaded connection portion 28, one end of the threaded connection portion 28 passes through the mounting hole and is connected with an upper fastening nut 31, the other end is connected with a lower fastening nut 32, a fastening sleeve 33 is also sleeved between the lower fastening nut 32 and the clamping part 29, the inner diameter of the fastening sleeve 33 is larger than the diameter of the upper end of the clamping part 29 and smaller than the diameter of the lower end of the clamping part 29, when the lower fastening nut 32 is rotated, the lower fastening nut 32 presses the fastening sleeve 33, the fastening sleeve 33 approaches to the clamping portion 29, the fastening sleeve 33 clamps the clamping block 30 to contract the clamping portion 29, the diameter of the capillary hole in the clamping portion 29 is reduced to fix the capillary 26, the capillary 26 penetrates through the clamping device 27, and the distance between two adjacent capillaries is 10 mm.

The X-axis driving mechanism comprises a first base body 2, a first stepping motor 5 and a first sliding table 1, a first mounting groove is formed in the first base body 2, a first concave groove is formed in the middle of the first sliding table 1, two ends of the first base body 2 are connected with first supporting plates 4 through bolts, the first supporting plates 4 are connected with first guide rods 3 through bolts, the first sliding table 1 is connected onto the first guide rods 3 in a sliding mode, a rotating portion of the first stepping motor 5 is connected with a lead screw nut mechanism, the lead screw nut mechanism is used for driving the first sliding table 1 to move along the X-axis direction, the first stepping motor 5 is mounted in the first mounting groove, and when the first sliding table 1 moves to the position of the first stepping motor 5, the first concave groove can accommodate the next first stepping motor 5; the Z-axis driving mechanism comprises a second seat body 7, a second stepping motor 10 and a second sliding table 6, a second mounting groove is formed in the second seat body 7, a second concave groove is formed in the middle of the second sliding table 6, two ends of the second seat body 7 are connected with second supporting plates 9 through bolts, the second supporting plates 9 are connected with second guide rods 8 through bolts, the second sliding table 6 is connected onto the second guide rods 8 in a sliding mode, the rotating portion of the second stepping motor 10 is connected with another screw nut mechanism, the screw nut mechanism is used for driving the second sliding table 6 to move along the Z-axis direction, the second stepping motor 10 is mounted in the second mounting groove, and when the second sliding table 6 moves to the position of the second stepping motor 10, the second concave groove can accommodate the second stepping motor 10; second pedestal 7 passes through slip table connecting piece 38 to be installed on first slip table 1 (first slip table 1, slip table connecting piece 38, second pedestal 7 all is provided with the screw, can be with first slip table 1 and slip table connecting piece 37 fixed connection through the bolt, the rethread bolt is with slip table connecting piece 38 and second pedestal 7 fixed connection, second pedestal 7 and first slip table 1 fixed connection have been realized promptly, capillary tube support 25 passes through bolted connection on second slip table 6, capillary tube support 25 is located test bench 21's top.

The cleaning test tube seat 24, the sponge layer 23, the reagent test tube seat 22 and the supporting plate 18 are arranged on the test bed 21 in sequence, the cleaning test tube seat 24 and the reagent test tube seat 22 are movably arranged on the test bed 21, reagents and cleaning liquid are convenient to replace, the cleaning test tube seat 24 and the reagent test tube seat 22 are provided with a plurality of round holes used for placing commercially available 'bullet' test tubes along the length direction, the distance between the axes of two adjacent round holes is set to be 10mm according to the diameter of the 'bullet' test tubes, the sponge layer 23 is fixed on the test bed 21 through glue, the supporting plate 18 is rotatably arranged on the test bed 21 (the two sides of the supporting plate 18 are fixedly provided with rotating shafts 34, the supporting plate 18 is rotatably arranged on the test bed 21 through the rotating shafts 34), the bottom of the supporting plate 18 is provided with a supporting plate slide rail 20, the bottom of the test bed 21 is provided with a bottom plate 17, the bottom plate 17 is provided with an unfolding cylinder 13 (the bottom plate 17 is provided with two opposite plates, a groove is formed between the two plates, the unfolding cylinder 13 is positioned in the groove and can prevent the position of the unfolding cylinder 13 from being unstable), the unfolding cylinder 13 is positioned at the bottom of one side of the supporting plate 18 and ensures that the supporting plate 18 can enter the unfolding cylinder 13 after rotating, the bottom plate 17 is fixedly provided with a rotating mechanism 11 (a steering engine can be selected) for driving the supporting plate 18 to rotate, the rotating part of the rotating mechanism 11 is connected with a rocker 14, the other end of the rocker 14 is rotatably connected with a T-shaped sliding block 37, the T-shaped sliding block 37 is slidably connected in a supporting plate sliding rail 20, and the T-shaped sliding block 37 has two freedom degrees of movement in the X-axis direction and the Y-axis direction in the supporting plate sliding rail 20 (the supporting plate sliding rail 20 is arranged in the X-axis direction, the width of the supporting plate sliding rail, the driving rotation mechanism 11 drives the rocker 14 to rotate, and the rocker 14 rotates to drive the T-shaped sliding block to move in the X-axis direction in the supporting plate sliding rail 20, so as to drive the supporting plate 18 to rotate; a moving mechanism 12 (a steering engine can be selected) for driving the supporting plate 18 to move along the Y axis is fixedly arranged on the bottom plate 17, the rotating part of the moving mechanism 12 is connected with an external gear 15, the external gear 15 is meshed with a rack 16, a connecting rod 39 is arranged on the rack, and the connecting rod 39 is rotatably connected to the rotating shaft 34; as shown in fig. 3, an abdicating groove 35 is provided on the supporting plate 18 at the connection position of the connecting rod 39 and the rotating shaft 34, a part of the connecting rod 39 is located in the abdicating groove 35, the abdicating groove 35 limits the connecting rod 39 in the axial direction of the rotating shaft 34 (i.e. the width of the abdicating groove 35 is slightly larger than the width of the connecting rod 39, the connecting rod 39 can only rotate in the abdicating groove 35 but cannot move along the Y-axis, so that the external gear 15 and the rack 16 which are engaged with each other when the supporting plate 18 rotates are not separated), a clamping plate 36 is also movably provided on the end of the supporting plate 18, the clamping plate 36 is connected to the supporting plate 18 through bolts, and when the supporting plate 18 is driven to rotate to drive the thin layer chromatography plate 40 to be immersed in the developing agent in.

As shown in FIG. 6, the device of the present invention uses STC12 as MCU, and starts to operate through micro-motion key or mobile phone Bluetooth control program. Firstly, an initialization program is executed, the first sliding table 1 and the second sliding table 6 are operated to an initialization position, the position is taken as a reference point, the first and second stepping motors are controlled to rotate by controlling pulse signals output to the first and second stepping motors, the screw nut mechanism converts the rotation of the first and second stepping motors into linear motion of the first and second sliding tables respectively, the capillary tube 26 is driven to be above the cleaning test tube seat 24 filled with a cleaning solution test tube by the motion of the first sliding table 1, then the second sliding table 6 descends to immerse the capillary tube 26 in the cleaning solution for 2s, the cleaning liquid is sucked into the capillary tube 26 by capillary effect, then the capillary tube 26 is moved to above the sponge layer 23, and then is lowered to contact the sponge, the cleaning liquid in the capillary tube 26 is sucked out through the sponge, this process is a cleaning process, and the capillary 26 may be considered to be cleaned after being repeatedly performed three times.

After the capillary tube 26 is cleaned, moving the capillary tube 26 to the position above the reagent test tube seat 22 filled with the sampling reagent, descending to immerse the capillary tube 26 in the reagent for 1s, moving the capillary tube 26 to the position above the supporting plate 18 after sampling is finished, then descending the capillary tube 26 to be in contact with the thin-layer chromatography plate clamped and fixed on the supporting plate 18 through the clamping plate 36 for 100ms, and sucking the reagent in the capillary tube by the thin-layer chromatography plate, wherein the process is a sampling point plate process.

After the operation of the sampling point plate is completed for one time, the distance between each sampling point on the thin-layer chromatography plate is 10mm, a vacant position for sampling point plates is left between the sampling points, in order to avoid the resource waste of the thin-layer chromatography plate, improve the use efficiency of the thin-layer chromatography plate, reduce the laboratory loss and save the cost, a reagent test tube seat 24 can be taken down to replace a new row of sampling reagents, then a supporting plate 18 is driven by a moving mechanism 12 to translate for 5mm along the Y-axis direction, then a cleaning and sampling point plate program is executed again, and after the program is executed, the distance between each sampling point on the thin-layer chromatography plate is 5 mm; and finally, driving the supporting plate 18 to rotate through the rotating mechanism 11, immersing the thin layer chromatography plate on the supporting plate 18 into the developing agent in the developing cylinder 13, immersing all sampling points in the developing agent, and observing a reaction result after waiting for a period of time.

The above is that the preferred embodiment of the present invention is only used for explaining the present invention, and is not used for limiting the present invention. All obvious changes or variations led by the technical proposal of the utility model are still within the protection scope of the utility model.

Claims (9)

1. The utility model provides an automatic reagent sampling thin layer chromatography device, its characterized in that, is including capillary frame (25), actuating mechanism and test bench (21) that are used for installing capillary (26), actuating mechanism is used for driving capillary frame (25) remove, capillary frame (25) set up test bench (21) top, be equipped with reagent test tube seat (22) of placing the test tube on test bench (21), be used for placing layer board (40) layer board (18) and be located exhibition jar (13) of layer board (18) one side bottom, still be provided with the drive respectively on test bench (21) rotatory and moving mechanism (11) and the moving mechanism (12) that remove layer board (18).

2. The automatic reagent sampling thin-layer chromatography device according to claim 1, wherein the driving mechanism comprises an X-axis driving mechanism and a Z-axis driving mechanism, the X-axis driving mechanism comprises a first base body (2), a first stepping motor (5) and a first sliding table (1), the first sliding table is slidably arranged on the first base body (2) along the X-axis direction through a lead screw nut mechanism, the first stepping motor (5) is used for driving the lead screw nut mechanism, the Z-axis driving mechanism comprises a second base body (7), a second stepping motor (10) and a second sliding table (6), the second base body (7) is arranged on the first sliding table (1), the second sliding table (6) is slidably arranged on the second base body (7) along the Z-axis direction through a lead screw nut mechanism, and the second stepping motor (10) is used for driving the lead screw nut mechanism, the capillary tube frame (25) is installed on the second sliding table (6).

3. The automatic reagent sampling thin-layer chromatography device according to claim 2, wherein a second concave groove is arranged in the middle of the second sliding table (6), a second mounting groove is arranged on the second base body (7), the second stepping motor (10) is fixedly connected in the mounting groove and positioned in the second concave groove, a second support plate (9) for connecting a second guide rod (8) is further arranged on the second base body (7), the second support plate (9) is fixedly connected at both ends of the second base body (7), the second sliding table (6) is slidably connected on the second guide rod (8), a first concave groove is arranged in the middle of the first sliding table (1), a first mounting groove is arranged on the first base body (2), the first stepping motor (5) is fixedly connected in the first mounting groove and positioned in the first concave groove, still be equipped with first backup pad (4) that are used for connecting first guide bar (3) on first pedestal (2), first backup pad (4) fixed connection be in the both ends of first pedestal (2), first slip table (1) sliding connection be in on first guide bar (3).

4. The automatic reagent sampling thin-layer chromatography device according to claim 1, wherein the capillary frame (25) is provided with a plurality of mounting holes along the length direction thereof, the distance between the axes of two adjacent mounting holes is 10mm, the mounting holes are internally provided with clamping devices (27), the clamping devices (27) are internally provided with capillary holes for mounting capillaries (26), each clamping device (27) comprises a threaded connection part (28) and a clamping part (29) positioned at the bottom of the threaded connection part (28), one end of the threaded connection part (28) penetrates through the mounting hole and is connected with an upper fastening nut (31), the other end of the threaded connection part is connected with a lower fastening nut (32), the clamping part (29) is a truncated cone-shaped structure formed by surrounding a plurality of clamping blocks (30), and a fastening sleeve (33) for fastening the clamping part (29) is further sleeved between the lower fastening nut (32) and the clamping part (29), the inner diameter of the fastening sleeve (33) is larger than the diameter of the upper end of the clamping part (29) and smaller than the diameter of the lower end of the clamping part (29).

5. The automatic reagent sampling thin-layer chromatography device according to claim 1, wherein a plurality of round holes for holding test tubes are arranged on the reagent tube seat (22) along the length direction of the reagent tube seat, the distance between the axes of two adjacent round holes is 10mm, the reagent tube seat (22) is movably arranged on the test bench (21), a cleaning tube seat (24) is also movably arranged on the test bench (21), the cleaning tube seat (24) is arranged in parallel with the reagent tube seat (22), a plurality of round holes for holding test tubes are arranged on the cleaning tube seat (24) along the length direction of the cleaning tube seat, the distance between the axes of two adjacent round holes is 10mm, and a sponge layer (23) is also arranged on the test bench (21) between the reagent tube seat (22) and the cleaning tube seat (24).

6. An automated reagent sampling thin layer chromatography device according to claim 1, characterized in that a bottom plate (17) is arranged below the test stand (21), and the test stand (21) is fixedly connected with the bottom plate (17) through a plurality of support rods (19).

7. The automatic reagent sampling thin-layer chromatography device according to claim 6, wherein a rotating mechanism (11) for driving the supporting plate (18) to rotate is fixedly arranged on the bottom plate (17), one end of a rocker (14) is connected to a rotating part of the rotating mechanism (11), the other end of the rocker (14) is rotatably connected with a T-shaped sliding block (37), the T-shaped sliding block (37) is slidably connected in a supporting plate sliding rail (20) and has two freedom degrees of movement in the X-axis direction and the Y-axis direction, the supporting plate sliding rail (20) is arranged at the bottom of the supporting plate (18), and the expanding cylinder (13) is mounted on the bottom plate (17).

8. The automatic reagent sampling thin-layer chromatography device according to claim 6, wherein a moving mechanism (12) for driving the supporting plate (18) to move along the length direction of the reagent test tube seat (22) is fixedly arranged on the bottom plate (17), an external gear (15) is arranged on a rotating part of the moving mechanism (12), the external gear (15) is meshed with a rack (16), the supporting plate (18) is rotatably arranged on the test bench (21) through a rotating shaft (34), the rack (16) is rotatably connected to the rotating shaft (34) through a connecting rod (39), an abdicating groove (35) is arranged on the supporting plate (18) at the joint of the connecting rod (39) and the rotating shaft (34), and the abdicating groove (35) limits the connecting rod (39) in the axial direction of the rotating shaft (34).

9. An automated reagent sampling thin-layer chromatography device according to claim 1, characterized in that a clamping plate (36) for fixing the thin-layer chromatography plate (40) is movably arranged on the supporting plate (18).

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