CN212199298U - Cell culture plate fixing device for detection - Google Patents
Cell culture plate fixing device for detection Download PDFInfo
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- CN212199298U CN212199298U CN202020604854.1U CN202020604854U CN212199298U CN 212199298 U CN212199298 U CN 212199298U CN 202020604854 U CN202020604854 U CN 202020604854U CN 212199298 U CN212199298 U CN 212199298U
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Abstract
The utility model discloses a fixing device of a cell culture plate for detection, which comprises a horizontal supporting part used for supporting and clamping the cell culture plate; the plate cover clamping part is arranged at one corner of the horizontal supporting part and is used for clamping the cell culture plate and the plate cover; and the hole site identification part is arranged on the horizontal supporting part, the upper surface of the hole site identification part is matched and attached with the bottom surface of the cell culture plate, and the hole site identification part is used for distinguishing the cell culture holes on the cell culture plate. The utility model has the advantages of simple structure and simple operation. The hole sites are clearly and accurately distinguished, the accurate addition of the reaction reagent is ensured, the reagent addition process is more time-saving and labor-saving, the visual wear of detection personnel is reduced, the phenomenon of repeated addition or addition omission is avoided, the accuracy of an experimental result is ensured, the detection cost is reduced, and the experimental failure is avoided; the clamping part of the plate cover can easily realize that the cell culture plate and the plate cover are clamped and fixed, effectively prevents a sample from being exposed in a natural environment, guarantees the sterility of the sample, and ensures the smooth development of an experiment.
Description
Technical Field
The utility model belongs to the technical field of the reagent detects technique and specifically relates to a cell culture plate fixing device is used in detection convenient to hole site is distinguished.
Background
At present, diagnostic reagents have wide application, and in the biomedical industry, the immunology, microbiology, molecular biology and other disciplines all relate to reagent detection. The detection and diagnosis are indispensable experimental processes in human diseases and epidemiology, and the conventional detection kit mostly adopts 96-well plates including an enzyme immunoassay plate, a chemiluminescence plate and the like, and even adopts a pyrogen-free 96-well cell culture plate in the detection process with higher requirements such as a peptidoglycan detection kit and the like.
The existing 96-well cell culture plate is made of optically transparent pure polystyrene, in the process of adding samples, the wells are not easy to distinguish, more reaction reagents (generally 5-10) are added in the detection process, and the addition amount in each reaction well is smaller (generally 10-50 microliter), so that the whole operation process is time-consuming and labor-consuming, the vision of detection personnel is seriously abraded, and the phenomenon of repeated addition or addition omission often occurs; once repeated addition or missing addition occurs, the detection result is abnormal, and the detection is carried out again until accurate data is obtained, so that samples are wasted, and the detection cost is greatly increased; if the abnormality cannot be found in time, the direction of the whole experiment is deviated, and the experiment fails.
In addition, in the whole detection, mixing and reaction processes of the existing 96-well cell culture plate, a specific fixing device is not arranged between the plate body and the plate cover, so that the plate cover is easy to separate, the sample is exposed in the natural environment, the sterility of the sample cannot be ensured, and the smooth development of the experiment is seriously influenced.
Disclosure of Invention
The utility model aims to provide a simple structure, easy and simple to handle's cell culture plate fixing device for detection.
In order to achieve the above purpose, the utility model can adopt the following technical proposal:
the cell culture plate fixing device for detection of the utility model comprises a horizontal supporting part for supporting and clamping the cell culture plate; the plate cover clamping part is arranged at one corner of the horizontal supporting part and is used for clamping the cell culture plate and the plate cover; and the hole site identification part is arranged on the horizontal supporting part, the upper surface of the hole site identification part is matched and attached with the bottom surface of the cell culture plate, and the hole site identification part is used for distinguishing the cell culture holes on the cell culture plate.
The horizontal supporting part comprises a horizontal bottom supporting plate, the size of the horizontal bottom supporting plate is matched with that of the cell culture plate, and side wall plates for clamping and fixing the cell culture plate are vertically and fixedly arranged at the peripheral edges of the upper surface of the horizontal bottom supporting plate; the middle parts of the side coamings positioned at the front side and the rear side of the horizontal bottom supporting plate are provided with grabbing openings which are convenient for taking and placing the cell culture plate.
The plate cover clamping part comprises a longitudinal mandrel which is vertically and fixedly arranged at one corner of the horizontal bottom supporting plate, a horizontally rotating sleeve is clamped on the longitudinal mandrel, a connecting arm which strides over the side wall plate is fixedly connected on the rotating sleeve, a horizontal pressing sheet with the lower surface attached to and pressed on the top surface of the plate cover of the cell culture plate is arranged at the outer end of the connecting arm, and the bottom surface of the horizontal pressing sheet is higher than the upper edge of the side wall plate; and a push handle which is convenient for pushing the horizontal pressing sheet to rotate horizontally is arranged on the horizontal pressing sheet.
The hole site identification part comprises a hole site identification card which is arranged on a horizontal bottom supporting plate at the inner side of the side wall plate in an adaptive manner, and hole site distinguishing identification color blocks which are in one-to-one correspondence with the cell culture holes on the cell culture plate are arranged on the upper surface of the hole site identification card; and the hole site identification card positioned at the outer side of the hole site distinguishing identification color block is correspondingly provided with a horizontal row number identification and a vertical row number identification.
The utility model has the advantages of simple structure and simple operation. When the cell culture plate is used for in vitro detection, the horizontal supporting part is used for ensuring the stable clamping of the cell culture plate, and the hole site identification part which is arranged on the horizontal supporting part and is matched and attached with the bottom surface of the cell culture plate is matched for clearly and accurately distinguishing hole sites, so that the accurate addition of a reaction reagent is ensured, the reagent addition process is more time-saving and labor-saving, the visual wear of detection personnel is reduced, the phenomenon of repeated addition or addition omission is avoided, the accuracy of an experimental result is ensured, the detection cost is reduced, and the experimental failure is avoided; in addition, the plate cover clamping part arranged at one corner of the horizontal bearing part can easily realize that the cell culture plate and the plate cover are clamped and fixed, the plate cover is prevented from being separated in the detection, mixing and reaction processes, the sample is effectively prevented from being exposed in the natural environment, the sterility of the sample is ensured, and the smooth development of the experiment is ensured.
Drawings
Fig. 1 is a schematic structural diagram of the present invention (the horizontal serial number marks and the vertical serial number marks are hidden).
Fig. 2 is a schematic structural diagram of the hole identification card of fig. 1.
Detailed Description
As shown in fig. 1 and 2, the fixing device for cell culture plate for detection of the present invention comprises a horizontal supporting portion for supporting and fixing the cell culture plate; the plate cover clamping part is arranged at one corner of the horizontal supporting part and is used for clamping the cell culture plate and the plate cover; and the hole site identification part is arranged on the horizontal supporting part, the upper surface of the hole site identification part is matched and attached with the bottom surface of the cell culture plate, and the hole site identification part is used for distinguishing the cell culture holes on the cell culture plate.
Specifically, the horizontal support part comprises a horizontal bottom support plate 1, the size of the horizontal bottom support plate 1 is matched with that of the cell culture plate, various sizes can be set according to cell culture plates of different models, taking a common 96-hole cell culture plate as an example, and the actual manufacturing length of the horizontal bottom support plate 1 is 127.6mm and the width is 85.75 mm. In order to stably fix the cell culture plate on the horizontal bottom supporting plate 1, side wall plates 2 for clamping and fixing the cell culture plate are vertically and fixedly arranged at the peripheral edges of the upper surface of the horizontal bottom supporting plate 1, and grabbing ports 3 convenient for taking and placing the cell culture plate are formed in the middle parts of the side wall plates 2 positioned at the front side and the rear side of the horizontal bottom supporting plate 1; in addition, the outer side surfaces of the side wall plates positioned at the front side and the rear side of the horizontal bottom supporting plate 1 can be further provided with frosted surfaces, so that an operator can grasp the sand conveniently when transferring the sand, and the friction force is increased.
The plate cover clamping part comprises a longitudinal mandrel 4 vertically and fixedly arranged at one corner of a horizontal bottom supporting plate 1, a horizontally rotating sleeve 5 is clamped on the longitudinal mandrel 4, a connecting arm 6 striding over from the upper part of a side wall plate 2 is fixedly connected on the rotating sleeve 5, and the outer end of the connecting arm 6 is provided with a horizontal pressing sheet 7 with the lower surface attached to and pressed on the top surface of a plate cover of the cell culture plate (namely the vertical distance between the horizontal pressing sheet 7 and the upper surface of the horizontal bottom supporting plate 1 is matched with the height of the cell culture plate buckled with the plate cover); in addition, the bottom surface of the horizontal pressing sheet 7 should be higher than the upper edges of the side coamings 2, and at least the bottom surface of the horizontal pressing sheet 7 should be higher than the upper edge of one of the two side coamings 2 close to the longitudinal mandrel 4, so as to ensure that the horizontal pressing sheet 7 can be easily rotated into or out of the upper part of the horizontal bottom supporting plate 1. For convenient operation, a push handle 8 is further disposed on the horizontal pressing plate 7 for pushing the horizontal pressing plate to rotate, and the push handle 8 may be a fixing plate vertically fixed on the top surface of the horizontal pressing plate 7. In addition, in order to improve the service durability of the device, the horizontal bottom supporting plate 1, the side wall plate 2 and the longitudinal mandrel 4 can be of an integrated structure made of resin or stainless steel materials, and the rotating sleeve 5, the connecting arm 6, the horizontal pressing plate 7 and the fixing plate can also be of an integrated structure made of resin or stainless steel materials and hinged on the longitudinal mandrel 4; and polishing treatment can be carried out on all edges of the horizontal bottom supporting plate 1, the side wall plate 2, the longitudinal core shaft 4, the rotating sleeve 5, the connecting arm 6, the horizontal pressing sheet 7 and the fixing plate, so that operators are prevented from being scratched when the device is used.
The hole site identification part comprises a hole site identification card 9 which is adapted to be placed on the horizontal bottom supporting plate 1 at the inner side of the side enclosing plate 2, and hole site distinguishing identification color blocks 10 which are in one-to-one correspondence with the cell culture holes on the cell culture plate are arranged on the upper surface of the hole site identification card 9; similarly, the hole site identification card 9 may also be set with various sizes according to cell culture plates of different models, taking a commonly used 96-well cell culture plate as an example, at this time, the actual manufacturing size of the hole site identification card should be consistent with the size of the horizontal bottom supporting plate 1, and also be 127.6mm long and 85.75mm wide, and at the same time, the hole site distinguishing identification color blocks 10 on the hole site identification card 9 should be correspondingly set to be 12 × 8 color blocks, and correspond to the cell culture wells of the 96-well cell culture plate one by one. In addition, the hole site distinguishes and marks the color lump 10 can also use the gradual change color of the same color to distinguish for the color lump in every horizontal row, and use different colors to distinguish between row and row, thus reach every cell culture hole of the cell culture plate can correspond to the color lump of a color, reduce the visual error to the minimum, the easy, clear, accurate distinguishing hole site, guarantee the accurate addition of the reaction reagent, lighten the eyesight abrasion of the inspector; in addition, a horizontal serial number mark 11 and a vertical serial number mark 12 can be correspondingly arranged on the hole site identification card 9 positioned outside the hole site distinguishing identification color block 10, and the hole site of any cell culture hole can be easily searched through the horizontal serial number mark 11 and the vertical serial number mark 12.
When adding samples, only a horizontal bottom supporting plate 1 with a proper size and a corresponding hole site identification card 9 are selected according to the requirements of a detection test, then the hole site identification card 9 is placed on the horizontal bottom supporting plate 1, then a corresponding cell culture plate is placed, the cell culture holes on the cell culture plate are ensured to be in one-to-one correspondence with the hole site distinguishing identification color blocks 10 on the hole site identification card 9, and then the cover can be opened for adding a reaction reagent; after the reaction reagent is added, the plate cover of the cell culture plate is buckled, the horizontal pressing sheet 7 is rotated to press and fix the cell culture plate and the plate cover, and then a series of subsequent operations such as transferring, uniformly mixing, reacting and the like can be carried out.
Claims (7)
1. The utility model provides a detect and use cell culture plate fixing device which characterized in that: comprises that
The horizontal supporting part is used for supporting and clamping the cell culture plate;
the plate cover clamping part is arranged at one corner of the horizontal supporting part and is used for clamping the cell culture plate and the plate cover; and
and the hole site identification part is arranged on the horizontal supporting part, the upper surface of the hole site identification part is matched and attached with the bottom surface of the cell culture plate, and the hole site identification part is used for distinguishing the cell culture holes on the cell culture plate.
2. The cell culture plate fixing device for assay according to claim 1, wherein: the horizontal supporting part comprises a horizontal bottom supporting plate, the size of the horizontal bottom supporting plate is matched with that of the cell culture plate, and side wall plates for clamping and fixing the cell culture plate are vertically and fixedly arranged at the peripheral edges of the upper surface of the horizontal bottom supporting plate.
3. The cell culture plate fixing device for assay according to claim 2, wherein: the middle parts of the side coamings positioned at the front side and the rear side of the horizontal bottom supporting plate are provided with grabbing openings which are convenient for taking and placing the cell culture plate.
4. The cell culture plate fixing device for assay according to claim 2, wherein: the plate cover clamping part comprises a longitudinal mandrel which is vertically and fixedly arranged at one corner of the horizontal bottom supporting plate, a horizontally rotating sleeve is clamped on the longitudinal mandrel, a connecting arm which strides over from the upper part of the side wall plate is fixedly connected on the rotating sleeve, a horizontal pressing sheet with a lower surface which is attached and pressed on the top surface of the plate cover of the cell culture plate is arranged at the outer end of the connecting arm, and the bottom surface of the horizontal pressing sheet is higher than the upper edge of the side wall plate.
5. The cell culture plate fixing device for assay according to claim 4, wherein: and a push handle which is convenient for pushing the horizontal pressing sheet to rotate horizontally is arranged on the horizontal pressing sheet.
6. The cell culture plate fixing device for assay according to claim 2, wherein: the hole site recognition part comprises a hole site identification card which is placed on a horizontal bottom supporting plate on the inner side of the side wall plate through an adapter card, and a hole site distinguishing identification color block which is in one-to-one correspondence with the cell culture holes on the cell culture plate is arranged on the upper surface of the hole site identification card.
7. The cell culture plate fixing device for assay according to claim 6, wherein: and the hole site identification card positioned at the outer side of the hole site distinguishing identification color block is correspondingly provided with a horizontal row number identification and a vertical row number identification.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020604854.1U CN212199298U (en) | 2020-04-21 | 2020-04-21 | Cell culture plate fixing device for detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020604854.1U CN212199298U (en) | 2020-04-21 | 2020-04-21 | Cell culture plate fixing device for detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212199298U true CN212199298U (en) | 2020-12-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020604854.1U Active CN212199298U (en) | 2020-04-21 | 2020-04-21 | Cell culture plate fixing device for detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN212199298U (en) |
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2020
- 2020-04-21 CN CN202020604854.1U patent/CN212199298U/en active Active
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