CN212152244U

CN212152244U - Adipose tissue small RNA extraction element

Info

Publication number: CN212152244U
Application number: CN202020218293.1U
Authority: CN
Inventors: 戴俊程; 江玥; 何元林
Original assignee: Hundred Million Co ltd Of Population Health Research Institute Of Section Of Nanjing
Current assignee: Hundred Million Co ltd Of Population Health Research Institute Of Section Of Nanjing
Priority date: 2020-02-27
Filing date: 2020-02-27
Publication date: 2020-12-15
Anticipated expiration: 2030-02-27

Abstract

The utility model discloses an adipose tissue small RNA extraction element, including organism and motor, the fixed surface that connecting rod and motor were passed through to the bottom of organism is connected, and the bottom of inner chamber of the body runs through first rotation post and second rotation post from interior to exterior in proper order, and the output fixed connection of shaft coupling and motor is passed through to the bottom of first rotation post, the utility model relates to an extract technical field. This adipose tissue small RNA extraction element, the internal surface of a bent section of thick bamboo is the peak valley form, carry out the breakage to the adipose tissue through a bent section of thick bamboo, first gear and second gear are in coordination, realize that first rotation post and second rotate post antiport's effect, thereby realize curb plate and side lever antiport's purpose, strengthen crushing effect, the silk screen reduces the resistance that the curb plate received, carry out the breakage to the adipose tissue through the silk screen simultaneously, further improve crushing effect, the problem that common adipose tissue small RNA extraction element is difficult to the guarantee to adipose tissue's crushing effect has been solved.

Description

Adipose tissue small RNA extraction element

Technical Field

The utility model relates to an extract technical field, specifically be an adipose tissue small RNA extraction element.

Background

Adipose tissue is composed of a large number of clustered fat cells, the clustered fat cells are divided into lobules by thin loose connective tissue, reticular fibers in the adipose tissue are well developed, the intercellular substance of lipid-protected tissue is few, fat at joints of the adipose tissue has the function of buffering muscular movement, fat at arms and soles helps to play a role of supporting pads, RNA (ribonucleic acid) is a genetic information carrier existing in biological cells and partial viruses and viroids, RNA is a chain molecule formed by condensation of ribonucleotide through a phosphodiester bond, and one ribonucleotide molecule is composed of phosphate, ribose and base.

When extracting adipose tissue small RNA, need carry out the breakage to adipose tissue, make small RNA and adipose tissue separation, then extract, but common extraction element is difficult to the guarantee to adipose tissue's crushing effect, be difficult to guarantee small RNA and adipose tissue complete segregation, the result of use is relatively poor, and common adipose tissue small RNA extraction element is difficult to guarantee the leakproofness, when extracting a small amount of small RNA, need the opening device, device inside and external direct contact, get into dust impurity easily, the internal environment of pollution device.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides an adipose tissue small RNA extraction element has solved common adipose tissue small RNA extraction element and has been difficult to ensure not enough to adipose tissue's crushing effect and leakproofness, receives the problem of pollution easily.

In order to achieve the above purpose, the utility model discloses a following technical scheme realizes: the utility model provides an adipose tissue small RNA extraction element, includes organism and motor, the fixed surface that connecting rod and motor were passed through to the bottom of organism is connected, the bottom of internal chamber of the engine has first rotation post and second rotation post from interior to outer running through in proper order, and the output fixed connection of shaft coupling and motor is passed through to the bottom of first rotation post, the fixed surface of first rotation post is connected with the curb plate, the side of curb plate is seted up flutedly, the side of curb plate runs through there is the silk screen, the side of first rotation post is passed through connecting rod fixedly connected with bent section of thick bamboo, the second rotates the fixed surface of post and is connected with the side lever.

Preferably, the outer surface of the first rotating column is fixedly connected with a first gear, the outer surface of the second rotating column is fixedly connected with a second gear, and the outer surface of the first gear is meshed with the outer surface of the second gear.

Preferably, the right side of the machine body is communicated with a C-shaped pipe, an inner cavity of the C-shaped pipe is fixedly connected with a first filter screen and a second filter screen from top to bottom in sequence, and the right side of the machine body is communicated with a side pipe communicated with the inner cavity of the C-shaped pipe.

Preferably, the right side of the C-shaped pipe is communicated with a fixed cylinder, the bottom of the fixed cylinder is communicated with an extraction pipe, and the inner cavity of the fixed cylinder is connected with a movable column through a sealing ring in a sliding manner.

Preferably, the side of the fixed cylinder is provided with an L-shaped groove, and the outer surface of the movable column is fixedly connected with an adjusting rod in sliding connection with the inner surface of the L-shaped groove.

Preferably, the bottom of the C-shaped pipe is fixedly connected with an air pump, the output end of the air pump is communicated with an air outlet pipe, the outer surface of the air outlet pipe is fixedly connected with a one-way valve, and the left end of the air outlet pipe penetrates through the side surface of the C-shaped pipe and extends into the C-shaped pipe.

Advantageous effects

The utility model provides an adipose tissue small RNA extraction element. Compared with the prior art, the method has the following beneficial effects:

(1) the adipose tissue small RNA extraction device comprises a body cavity, wherein a first rotating column and a second rotating column sequentially penetrate through the bottom of the body cavity from inside to outside, the bottom end of the first rotating column is fixedly connected with the output end of a motor through a coupler, a side plate is fixedly connected to the outer surface of the first rotating column, a groove is formed in the side surface of the side plate, a silk screen penetrates through the side surface of the side plate, a curved cylinder is fixedly connected to the side surface of the first rotating column through a connecting rod, a side rod is fixedly connected to the outer surface of the second rotating column, a first gear is fixedly connected to the outer surface of the first rotating column, a second gear is fixedly connected to the outer surface of the second rotating column, the outer surface of the first gear is meshed with the outer surface of the second gear, the inner surface of the curved cylinder is in a peak valley shape, adipose tissues passing through the curved cylinder are crushed, and the first gear and the second gear cooperate with each other to realize the effect of reverse rotation of the first, thereby realize curb plate and side lever antiport's purpose, strengthen crushing effect, the silk screen reduces the resistance that the curb plate received, carries out the breakage to the adipose tissue through the silk screen simultaneously, further improves crushing effect, has solved common adipose tissue small RNA extraction element and has been difficult to the problem of guarantee to adipose tissue's crushing effect.

(2) The bottom of the fixed cylinder is communicated with the extraction pipe, the inner cavity of the fixed cylinder is connected with the movable column in a sliding way through the sealing ring, the side surface of the fixed cylinder is provided with the L-shaped groove, the outer surface of the movable column is fixedly connected with the adjusting rod in a sliding way with the inner surface of the L-shaped groove, the L-shaped groove is convenient for the adjusting rod to move, meanwhile, the position of the adjusting rod can be conveniently fixed, the position of the movable column can be adjusted through the adjusting rod, thereby adjust the position of two sealing washers, the left side sealing washer seals the extraction tube left side position in to the solid fixed cylinder, the extraction of the liquid of being convenient for, and the right side sealing washer seals extraction tube right side position in to the solid fixed cylinder, ensures that the outside air can not get into the extraction tube through the solid fixed cylinder, has solved common adipose tissue small RNA extraction element and has been difficult to ensure the leakproofness, receives the problem of pollution easily.

Drawings

Fig. 1 is a schematic view of the internal structure of the present invention;

FIG. 2 is a cross-sectional view of the fixed cylinder structure of the present invention;

FIG. 3 is a schematic view of the structural connection between the first rotary column and the curved cylinder of the present invention;

fig. 4 is a schematic view of the structural connection between the first gear and the second gear of the present invention.

In the figure: 1. a body; 2. a motor; 3. a first rotating column; 4. a second rotating cylinder; 5. a side plate; 6. a groove; 7. a wire mesh; 8. a curved cylinder; 9. a side lever; 10. a first gear; 11. a second gear; 12. a C-shaped pipe; 13. a first filter screen; 14. a second filter screen; 15. a side tube; 16. a fixed cylinder; 17. an extraction pipe; 18. moving the column; 19. a seal ring; 20. an L-shaped groove; 21. adjusting a rod; 22. an air pump; 23. an air outlet pipe; 24. a one-way valve.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides a technical solution: an adipose tissue small RNA extraction device comprises a machine body 1 and a motor 2, wherein the top of the machine body 1 is communicated with a feeding pipe, the side surface of the machine body 1 is communicated with a discharging pipe, the motor 2 is electrically connected with an external power supply and is controlled by a control switch, the bottom of the machine body 1 is fixedly connected with the outer surface of the motor 2 through a connecting rod, a first rotating column 3 and a second rotating column 4 sequentially penetrate through the bottom of an inner cavity of the machine body 1 from inside to outside, the four second rotating columns 4 are arranged and are uniformly distributed around the second rotating column 4, the bottom end of the first rotating column 3 is fixedly connected with the output end of the motor 2 through a coupler, the outer surface of the first rotating column 3 is fixedly connected with a side plate 5, the side surface of the side plate 5 is provided with a groove 6, the side surface of the side plate 5 is penetrated with a silk screen 7, the side surface of the first rotating column 3 is fixedly connected with a curved cylinder 8 through a, the adipose tissues passing through the curved tube 8 are crushed, the outer surface of the second rotating column 4 is fixedly connected with a side rod 9, the groove 6 ensures that the side plate 5 and the side rod 9 do not collide with each other when the side plate 5 and the side rod 9 rotate, the outer surface of the first rotating column 3 is fixedly connected with a first gear 10, the outer surface of the second rotating column 4 is fixedly connected with a second gear 11, the outer surface of the first gear 10 is meshed with the outer surface of the second gear 11, the right side of the machine body 1 is communicated with a C-shaped tube 12, the inner cavity of the C-shaped tube 12 is fixedly connected with a first filter screen 13 and a second filter screen 14 from top to bottom in sequence, the right side of the machine body 1 is communicated with a side tube 15 communicated with the inner cavity of the C-shaped tube 12, the side tube 15 is provided with a plurality of side tubes, so that liquid at a plurality of height positions in the machine body 1 can enter the C-shaped tube 12, the, the inner chamber of solid fixed cylinder 16 has removal post 18 through sealing washer 19 sliding connection, sealing washer 19 sets up two, L type groove 20 has been seted up to solid fixed cylinder 16's side, the outer fixed surface who removes post 18 is connected with the regulation pole 21 with the internal surface sliding connection in L type groove 20, the bottom fixedly connected with air pump 22 of C type pipe 12, air pump 22 and external power source electric connection, control through control switch, the output intercommunication of air pump 22 has outlet duct 23, and the outer fixed surface of outlet duct 23 is connected with check valve 24, the left end of outlet duct 23 runs through the side of C type pipe 12 and extends to the inside of C type pipe 12.

And those not described in detail in this specification are well within the skill of those in the art.

When the device is used, adipose tissue fluid is put into the machine body 1 through the feed port, the feed port is closed, the motor 2 is started, the motor 2 drives the first rotating column 3 to rotate, the first rotating column 3 drives the side plate 5 and the curved cylinder 8 to rotate, the side plate 5 drives the silk screen 7 to rotate, the first rotating column 3 drives the second rotating column 4 to rotate through the first gear 10 and the second gear 11, the second rotating column 4 drives the side rod 9 to rotate, the adipose tissue fluid is stirred and crushed, the air pump 22 is started, the air pump 22 blows air into the C-shaped tube 12 through the air outlet pipe 23 rapidly, so that the fluid in the C-shaped tube 12 flows clockwise, other impurities are filtered when the fluid enters the first filter screen 13, the fluid containing small RNA passes through the first filter screen 13, when the fluid passes through the second filter screen 14, the second filter screen 14 blocks the small RNA in the fluid, so that the small RNA stays between the first filter screen 13 and the second filter screen 14, when small RNA needs to be extracted, the adjusting rod 21 is rotated to enable the adjusting rod 21 to be in a vertical state, the adjusting rod 21 drives the moving column 18 to move towards the right, the moving column 18 drives the two sealing rings 19 to move towards the right, and when the left sealing ring 19 moves to the right side of the extracting pipe 17, the small RNA is extracted through the extracting pipe 17.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides an adipose tissue small RNA extraction element, includes organism (1) and motor (2), the surface fixed connection of connecting rod and motor (2) is passed through to the bottom of organism (1), its characterized in that: organism (1) inner chamber's bottom runs through first rotation post (3) and second from interior to exterior in proper order and rotates post (4), and the output fixed connection of shaft coupling and motor (2) is passed through to the bottom of first rotation post (3), the external fixed surface of first rotation post (3) is connected with curb plate (5), the side of curb plate (5) is seted up flutedly (6), silk screen (7) have been run through to the side of curb plate (5), the side of first rotation post (3) is through the crooked section of thick bamboo (8) of connecting rod fixedly connected with, the external fixed surface of second rotation post (4) is connected with side lever (9).

2. The apparatus for extracting small RNA from adipose tissues according to claim 1, wherein: the outer surface of the first rotating column (3) is fixedly connected with a first gear (10), the outer surface of the second rotating column (4) is fixedly connected with a second gear (11), and the outer surface of the first gear (10) is meshed with the outer surface of the second gear (11).

3. The apparatus for extracting small RNA from adipose tissues according to claim 1, wherein: the right side of the machine body (1) is communicated with a C-shaped pipe (12), the inner cavity of the C-shaped pipe (12) is fixedly connected with a first filter screen (13) and a second filter screen (14) from top to bottom in sequence, and the right side of the machine body (1) is communicated with a side pipe (15) communicated with the inner cavity of the C-shaped pipe (12).

4. The apparatus for extracting small RNA from adipose tissues according to claim 3, wherein: the right side of the C-shaped pipe (12) is communicated with a fixed cylinder (16), the bottom of the fixed cylinder (16) is communicated with an extraction pipe (17), and the inner cavity of the fixed cylinder (16) is connected with a movable column (18) in a sliding mode through a sealing ring (19).

5. The apparatus for extracting small RNA from adipose tissues according to claim 4, wherein: an L-shaped groove (20) is formed in the side face of the fixed cylinder (16), and an adjusting rod (21) which is connected with the inner surface of the L-shaped groove (20) in a sliding mode is fixedly connected to the outer surface of the movable column (18).

6. The apparatus for extracting small RNA from adipose tissues according to claim 3, wherein: the bottom fixedly connected with air pump (22) of C type pipe (12), the output intercommunication of air pump (22) has outlet duct (23), and the external fixed surface of outlet duct (23) is connected with check valve (24), the left end of outlet duct (23) runs through the side of C type pipe (12) and extends to the inside of C type pipe (12).