CN114870428B - Production device and working method of preparation for promoting synthesis of gardenia crocin - Google Patents
Production device and working method of preparation for promoting synthesis of gardenia crocin Download PDFInfo
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- CN114870428B CN114870428B CN202210368633.2A CN202210368633A CN114870428B CN 114870428 B CN114870428 B CN 114870428B CN 202210368633 A CN202210368633 A CN 202210368633A CN 114870428 B CN114870428 B CN 114870428B
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- 238000002360 preparation method Methods 0.000 title claims abstract description 53
- SEBIKDIMAPSUBY-ARYZWOCPSA-N Crocin Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)C(C)=CC=CC(C)=C\C=C\C=C(/C)\C=C\C=C(C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O1)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SEBIKDIMAPSUBY-ARYZWOCPSA-N 0.000 title claims abstract description 45
- SEBIKDIMAPSUBY-JAUCNNNOSA-N Crocin Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C(=O)OC1OC(COC2OC(CO)C(O)C(O)C2O)C(O)C(O)C1O)C=CC=C(/C)C(=O)OC3OC(COC4OC(CO)C(O)C(O)C4O)C(O)C(O)C3O SEBIKDIMAPSUBY-JAUCNNNOSA-N 0.000 title claims abstract description 45
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 28
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 27
- 230000001737 promoting effect Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 15
- 240000001972 Gardenia jasminoides Species 0.000 title 1
- 239000002994 raw material Substances 0.000 claims abstract description 59
- 238000010828 elution Methods 0.000 claims abstract description 54
- 241000157835 Gardenia Species 0.000 claims abstract description 44
- 238000000926 separation method Methods 0.000 claims abstract description 38
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 238000000605 extraction Methods 0.000 claims abstract description 10
- 238000005086 pumping Methods 0.000 claims abstract description 10
- 238000003860 storage Methods 0.000 claims abstract description 9
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 27
- 239000000243 solution Substances 0.000 claims description 20
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 18
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 12
- TWCMVXMQHSVIOJ-UHFFFAOYSA-N Aglycone of yadanzioside D Natural products COC(=O)C12OCC34C(CC5C(=CC(O)C(O)C5(C)C3C(O)C1O)C)OC(=O)C(OC(=O)C)C24 TWCMVXMQHSVIOJ-UHFFFAOYSA-N 0.000 claims description 10
- PLMKQQMDOMTZGG-UHFFFAOYSA-N Astrantiagenin E-methylester Natural products CC12CCC(O)C(C)(CO)C1CCC1(C)C2CC=C2C3CC(C)(C)CCC3(C(=O)OC)CCC21C PLMKQQMDOMTZGG-UHFFFAOYSA-N 0.000 claims description 10
- PFOARMALXZGCHY-UHFFFAOYSA-N homoegonol Natural products C1=C(OC)C(OC)=CC=C1C1=CC2=CC(CCCO)=CC(OC)=C2O1 PFOARMALXZGCHY-UHFFFAOYSA-N 0.000 claims description 10
- 230000001360 synchronised effect Effects 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 claims description 6
- 239000000049 pigment Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- PBCJIPOGFJYBJE-UHFFFAOYSA-N acetonitrile;hydrate Chemical compound O.CC#N PBCJIPOGFJYBJE-UHFFFAOYSA-N 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 239000003480 eluent Substances 0.000 claims description 3
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- 238000011068 loading method Methods 0.000 claims description 3
- 238000004587 chromatography analysis Methods 0.000 claims description 2
- 230000001588 bifunctional effect Effects 0.000 claims 1
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- 230000001580 bacterial effect Effects 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 6
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- 235000015334 Phyllostachys viridis Nutrition 0.000 description 4
- 239000011425 bamboo Substances 0.000 description 4
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- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 244000124209 Crocus sativus Species 0.000 description 3
- 235000015655 Crocus sativus Nutrition 0.000 description 3
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- 230000012010 growth Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- RGSFGYAAUTVSQA-UHFFFAOYSA-N Cyclopentane Chemical compound C1CCCC1 RGSFGYAAUTVSQA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
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- 241001474374 Blennius Species 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 241000596148 Crocus Species 0.000 description 1
- 235000004237 Crocus Nutrition 0.000 description 1
- 241001113425 Iridaceae Species 0.000 description 1
- 239000012880 LB liquid culture medium Substances 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 102000008579 Transposases Human genes 0.000 description 1
- 108010020764 Transposases Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
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- DMEGYFMYUHOHGS-UHFFFAOYSA-N heptamethylene Natural products C1CCCCCC1 DMEGYFMYUHOHGS-UHFFFAOYSA-N 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
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- 229910052742 iron Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
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- 229920001282 polysaccharide Polymers 0.000 description 1
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- 239000011148 porous material Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- 229960005486 vaccine Drugs 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0215—Solid material in other stationary receptacles
- B01D11/0223—Moving bed of solid material
- B01D11/0238—Moving bed of solid material on fixed or rotating flat surfaces, e.g. tables combined with rotating elements or on rotating flat surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Saccharide Compounds (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention discloses a production device of a preparation for promoting gardenia crocin synthesis, which comprises an elution barrel, wherein a raw material separation barrel is arranged in the elution barrel, separation holes are formed in the raw material separation barrel in an annular equidistant array, a dual-function connecting part is arranged between the outer bottom of the raw material separation barrel and the inner bottom surface of the elution barrel, at least six groups of liquid inlet pipes are arranged in the annular equidistant array at the position, close to the top, of the elution barrel, one end of each liquid inlet pipe extends to the inner bottom surface of the elution barrel and does not contact with the inner bottom surface of the elution barrel, a preparation storage cavity is formed between the elution barrel and the raw material separation barrel, and a pumping pipe is inserted in the preparation storage cavity; meanwhile, the invention also discloses a working method of a production device of the preparation for promoting the synthesis of the gardenia crocin, endophytes with synergistic promoting effect are used as main materials for compounding, and the total sum of the preparation for promoting the synthesis of the gardenia crocin is found, so that the extraction method of the crocin and the acquisition range of a carrier are enlarged.
Description
Technical Field
The invention relates to the technical field of crocin pigment synthesis agents, in particular to a production device and a working method of a preparation for promoting crocin pigment synthesis of gardenia.
Background
Phosphorus, potassium, calcium, magnesium, iron, zinc, boron and the like are essential elements for plant fruit growth, promote the enlargement development and uniform coloring of fruits, and can be used as a natural soil conditioner to directly supplement nutrient elements which are missing in soil, activate beneficial microorganism bacteria and inhibit harmful bacteria. For example, chitin: activating cells, having the function of a plant vaccine, strengthening the resistance of fruit trees to bad natural environment, inducing to generate resistance factors to diseases, and thus reducing the occurrence rate of the diseases; cyclopentane polyhydrophenanthrene: the plant growth regulator has important roles in the growth and development of plants, is involved in regulating and controlling a plurality of aspects of plant development, and has important roles in the defense of the plants against environmental stress; seaweed extract: is rich in 18 protein amino acids and algal polysaccharide which can be directly absorbed and utilized by plants, can promote the formation of soil aggregate structure and improve the internal pore space of soil.
Crocus sativus is a plant of the genus crocus of the family Iridaceae, native to the south of Europe to Iran, and transferred into the Tibet via India, so is also called crocus sativus, and is cultivated in small amounts in Zhejiang, jiangxi, jiangsu, beijing and Shanghai in China. It is not only a rare medicinal material, but also a rare spice, and modern pharmacological research shows that crocus sativus has strong circulatory system function and biological activities such as anti-tumor, antioxidation, etc. The dry column head of the Chinese medicine is used as a medicine, namely the stigma croci, and has the effects of activating blood circulation to dissipate blood stasis, cooling blood to remove toxicity, resolving depression and soothing nerves. Crocin pigment, also called crocin pigment, refers to the general name of carotenoid, crocin and other pigments extracted from crocin stigma.
Because of its low extraction carrier content, there is a need for a preparation for crocin synthesis and a method for operating a preparation production apparatus.
Disclosure of Invention
The invention aims to provide a production device of a preparation for synthesizing the crocin of the fructus gardeniae, and aims to find the total sum of the preparation for synthesizing the crocin of the fructus gardeniae, increase the extraction method of the crocin and the acquisition range of a carrier so as to solve the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the production device of the preparation for promoting the synthesis of the gardenia crocin comprises an elution barrel, wherein a raw material separation barrel is arranged in the elution barrel, separation holes are formed in the raw material separation barrel in an annular equidistant array manner, a dual-function connecting part is arranged between the outer bottom of the raw material separation barrel and the inner bottom surface of the elution barrel, at least six groups of liquid inlet pipes are arranged in the annular equidistant array manner at the position, close to the top, of the elution barrel, one end of each liquid inlet pipe extends to the inner bottom surface of the elution barrel and is not contacted with the inner bottom surface of the elution barrel, a preparation storage cavity is formed between the elution barrel and the raw material separation barrel, and a pumping pipe is inserted in the preparation storage cavity;
a lifting assembly is arranged at the top of the raw material separation cylinder;
the top of the elution barrel is provided with a barrel cover, the upper surface of the barrel cover is provided with a rotary driving assembly, and the rotary driving assembly is in transmission connection with the raw material separation barrel;
the bottom surface of the raw material separating cylinder is fixedly provided with a positioning shaft, the surface of the positioning shaft is fixedly provided with a linear bearing, the inner bottom surface of the eluting cylinder is fixedly provided with a positioning pipe, and the positioning shaft is inserted into the positioning pipe in a sliding manner;
limiting plates are symmetrically arranged on two sides of the cylinder cover, limiting grooves are symmetrically formed on two sides of the elution cylinder, the limiting plates are in clearance fit with the limiting grooves, a limiting ring is fixedly connected to the inner bottom surface of the cylinder cover, a turntable bearing is fixedly arranged on the inner side of the limiting ring, the limiting ring is in clearance fit with the raw material separation cylinder, and rubber base plates are arranged on the tops of the limiting ring and the raw material separation cylinder;
the lifting assembly comprises a support, the support is arranged on one side of the elution barrel, two groups of electric push rods are symmetrically arranged at the top of the support, a connecting joint is fixedly arranged at the end of a telescopic rod of each electric push rod, and the connecting joint is fixedly connected with the upper surface of the barrel cover.
The liquid inlet pipe comprises a liquid inlet pipe, a liquid outlet pipe and a liquid outlet pipe, wherein the liquid inlet pipe is characterized in that the liquid inlet pipe is fixedly provided with a valve at the pipe orifice, and the pipe orifice of the liquid inlet pipe is provided with identification patches, and the identification patches correspond to preparations of different categories.
The rotary driving assembly comprises a driving motor, the driving motor is fixedly arranged on the upper surface of the cylinder cover, a linkage shaft is fixedly arranged at the middle position in the raw material separating cylinder, synchronous pulleys are fixedly arranged at the top end of the linkage shaft and the output shaft end of the driving motor, and a synchronous belt is connected between the two groups of synchronous pulleys in a transmission manner.
The device comprises a barrel cover, a flow meter, a preparation collecting barrel, a flow meter, a preparation collecting barrel and a preparation collecting barrel, wherein the flow meter is arranged in the extraction pipe, one end of the extraction pipe penetrates through the barrel cover and extends into the elution barrel, the preparation collecting barrel is arranged on one side of the barrel cover, and the other end of the extraction pipe extends into the preparation collecting barrel.
The invention also provides a technical scheme, a working method of a production device of the preparation for promoting the synthesis of the gardenia crocin, which comprises the following steps:
s1, loading a raw product containing a gardenia raw material extract into a raw material separation barrel, respectively adding eluent of ethyl acetate, methanol and water into the elution barrel through a liquid inlet pipe attached with a label, and eluting the gardenia raw material, wherein the ethyl acetate: methanol: the proportion of water is 15-20:20-3.5:0.05-0.25, in the elution process, starting a driving motor to drive a linkage shaft so as to drive a raw material separation cylinder to rotate, and detecting the crocin aglycone content in the gardenia by HPLC (high performance liquid chromatography) after sufficient elution;
s2, the eluting concentrated solution in the step S1 is left in an eluting cylinder, the gardenia raw material in the raw material separating cylinder is taken out by utilizing a lifting component, the eluting concentrated solution is reserved, an acetonitrile water solution with the concentration of 15-22% is continuously poured into the eluting cylinder through a liquid inlet pipe for continuous eluting until a secondary concentrated solution is obtained, and the crocin aglycone content in the gardenia is detected by HPLC (high performance liquid chromatography) method;
s3, taking out the secondary concentrated solution through a pumping pipe to a preparation collecting cylinder, fusing with 40-60% methanol aqueous solution at 5-15.0 Mpa pressure, removing a small amount of front and rear impurities, and collecting the preparation for promoting the synthesis of the gardenia crocin.
Wherein the eluting pressure in the eluting cylinder used in the step S2 is 0.05-0.55 Mpa.
In summary, the beneficial effects of the invention are as follows due to the adoption of the technology:
in the invention, the content of crocin aglycone in the gardenia is detected by HPLC chromatography: 13.21g/100g; meanwhile, the gardenia endophyte with the biological synthesis effect on crocin aglycone is screened, double-bacteria combination is carried out on the gardenia endophyte, the endophyte with the synergistic promotion effect is used as a main material for compounding, and the total preparation for promoting the synthesis of crocin of the gardenia is found, so that the extraction method of crocin and the acquisition range of a carrier are enlarged;
according to the invention, the elution barrel and the raw material separation barrel are sequentially arranged, the raw material separation barrel is driven by the driving component to rotate, the gardenia raw material and the preparation are better fused fully for elution, and meanwhile, the lifting component is arranged, so that the raw material separation barrel is rapidly taken out, the raw material is separated, the addition and the reaction of the secondary elution preparation are ensured, the elution efficiency is improved, and the crocin is rapidly obtained.
Drawings
FIG. 1 is a schematic perspective view showing the structure of a device for producing a preparation for promoting the synthesis of gardenia crocin according to the present invention.
FIG. 2 is a schematic cross-sectional perspective view of an elution column of a production apparatus for a preparation for promoting the synthesis of gardenia crocin according to the present invention.
FIG. 3 is a perspective view showing the structure of the bottom surface of the cap of the apparatus for producing a preparation for promoting the synthesis of gardenia crocin according to the present invention.
FIG. 4 is a working flow chart of a production apparatus of the agent for promoting the synthesis of gardenia crocin according to the present invention.
In the figure: 1. an elution drum; 101. a cylinder cover; 102. a positioning tube; 103. a limiting plate; 104. a limit groove; 105. a limiting ring; 106. a turntable bearing; 107. a rubber backing plate; 2. a raw material separation cylinder; 201. a separation hole; 202. positioning a shaft; 3. a dual function connection; 4. a liquid inlet pipe; 5. a formulation storage cavity; 6. a drawing-off pipe; 601. a flow meter; 7. a lifting assembly; 701. a bracket; 702. an electric push rod; 703. a connection joint; 8. a rotary drive assembly; 801. a driving motor; 802. a linkage shaft; 9. a preparation collecting cylinder.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "orientation" or "positional relationship" are based on the orientation or positional relationship shown in the drawings, and are merely for convenience of description and to simplify the description, rather than to indicate or imply that the apparatus or elements referred to must have a particular orientation, be constructed and operate in a particular orientation, and therefore should not be construed as limiting the invention.
In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art based on the specification.
Example 1
The invention provides a production device of a preparation for promoting gardenia crocin synthesis, which is shown in figures 1-3, and comprises an elution barrel 1, wherein a raw material separation barrel 2 is arranged in the elution barrel 1, separation holes 201 are annularly and equidistantly arranged in the raw material separation barrel 2, a dual-function connecting part 3 is arranged between the outer bottom of the raw material separation barrel 2 and the inner bottom surface of the elution barrel 1, at least six groups of liquid inlet pipes 4 are annularly and equidistantly arranged at the position, close to the top, of the elution barrel 1, one end of each liquid inlet pipe 4 extends to the inner bottom surface of the elution barrel 1 and is not contacted with the inner bottom surface of the elution barrel 1, a preparation storage cavity 5 is arranged between the elution barrel 1 and the raw material separation barrel 2, a pumping pipe 6 is inserted in the preparation storage cavity 5, a flow meter 601 is arranged in the pumping pipe 6, one end of the pumping pipe 6 penetrates through a barrel cover 101 and extends into the elution barrel 1, one side of the barrel cover 101 is provided with a preparation collection barrel 9, and the other end of the pumping pipe 6 extends into the preparation collection barrel 9.
The bottom surface of the raw material separation cylinder 2 is fixedly provided with a positioning shaft 202, the surface of the positioning shaft 202 is fixedly provided with a linear bearing, the inner bottom surface of the elution cylinder 1 is fixedly provided with a positioning tube 102, and the positioning shaft 202 is in sliding insertion connection with the positioning tube 102.
The mouth of the liquid inlet pipe 4 is fixedly provided with a valve, and the mouth of the liquid inlet pipe 4 is provided with a label which corresponds to different types of preparations.
The utility model discloses a washing and dehydrating device, including barrel cover 101, rubber backing plate 107, barrel cover 101, barrel cover 2, stopper 103, stopper 104, stopper 103 and stopper 104 clearance fit are offered to barrel cover 101 bilateral symmetry, bottom surface fixedly connected with spacing ring 105 in the barrel cover 101, the inboard fixed mounting of spacing ring 105 has carousel bearing 106, spacing ring 105 and raw materials separating tube 2 clearance fit, and spacing ring 105 and raw materials separating tube 2 top are provided with rubber backing plate 107.
The utility model discloses a washing and drying device, including a raw materials separating section of thick bamboo 2, a raw materials separating section of thick bamboo 2 top is provided with hoisting assembly 7, hoisting assembly 7 includes support 701, support 701 sets up in elution section of thick bamboo 1 one side, support 701 top symmetry is provided with two sets of electric putter 702, the telescopic link end fixed mounting of electric putter 702 has attach fitting 703, attach fitting 703 and cover 101 upper surface fixed connection.
The elution section of thick bamboo 1 top is provided with cover 101, cover 101 upper surface is provided with rotary drive subassembly 8, rotary drive subassembly 8 and raw materials separating drum 2 transmission are connected, rotary drive subassembly 8 includes driving motor 801, driving motor 801 fixed mounting is in cover 101 upper surface, central point puts fixed mounting in the raw materials separating drum 2 has universal driving shaft 802, the equal fixed mounting of output shaft end of universal driving shaft 802 top and driving motor 801 has synchronous pulleys, and the transmission is connected with the hold-in range between two sets of synchronous pulleys.
As shown in FIG. 4, the working method of the production device of the preparation for promoting the synthesis of gardenia crocin comprises the following steps:
s1, loading a raw product containing a gardenia raw material extract into a raw material separation barrel 2, respectively adding eluent of ethyl acetate, methanol and water into an elution barrel 1 through a liquid inlet pipe 4 attached with a label, and eluting the gardenia raw material, wherein the ethyl acetate: methanol: the proportion of water is 15-20:20-3.5:0.05-0.25, in the elution process, starting a driving motor 801 to drive a linkage shaft 802, so as to drive a raw material separation cylinder 2 to rotate, and detecting the crocin aglycone content in the gardenia through an HPLC (high performance liquid chromatography) after sufficient elution;
s2, the eluting concentrated solution in the step S1 is left in the eluting barrel 1, the gardenia raw material in the raw material separating barrel 2 is taken out by utilizing the lifting component 7, the eluting concentrated solution is reserved, the eluting is continuously carried out by filling acetonitrile water solution with the concentration of 15-22% into the eluting barrel 1 through the liquid inlet pipe 4 until secondary concentrated solution is obtained, and the crocin aglycone content in the gardenia is detected by HPLC (high performance liquid chromatography);
s3, taking out the secondary concentrated solution through a pumping pipe 6 to a preparation collecting cylinder 9, fusing the secondary concentrated solution with 40-60% methanol aqueous solution at the pressure of 5-15.0 Mpa, removing a small amount of front and rear impurities, and collecting the preparation for promoting the synthesis of the gardenia crocin.
Specifically, the eluting pressure in the eluting tube 1 used in the step S2 is 0.05-0.55 Mpa.
Example 2
Pouring the melted culture medium into a sterile plate by adopting a coating plate method to prepare a sterile plate, selecting gardenia endophyte strain for culturing after cooling and solidifying, dripping a certain amount of strain suspension on the surface of the plate, uniformly dispersing the bacterial liquid on the surface of the whole plate by using a sterile glass coating rod for culturing, and separating and purifying various strains;
gene sequencing of the above Strain
Extracting DNA of the endophyte, preparing quality control index, adopting a transposase method to fragment the DNA and connect barcode after meeting the standard, mixing the libraries according to a proportion, connecting sequencing joints, and finally accurately quantifying the DNA library by using Qubit. After library construction was completed, a concentration and volume of DNA library was added to each individual Flow cell and the Flow cells were transferred to a GridION X5 sequencer for real-time single molecule sequencing.
Strains regulating crocin aglycone genes were selected by comparative genomic analysis by gene sequencing. Thereafter, the growth curves of these several strains in gardenia were monitored dynamically. After the bacterial strain 1 decays, the number of the bacterial strain 2 is rapidly reduced through curve discovery, and then the two bacterial strains are found to have a synergistic relationship after exploration, so that a designed experiment is obtained, a single bacterial strain and double bacterial strains are combined in a gardenia plant for experiment, the result is judged through measuring the quality of gardenia fruits and the content of pigmentary aglycone, after the bacterial strain is determined, the bacterial colony is picked up and inoculated in a new LB liquid culture medium for shake flask culture, and the culture is expanded.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Claims (6)
1. The production device of the preparation for promoting gardenia crocin synthesis is characterized by comprising an elution barrel (1), wherein a raw material separation barrel (2) is arranged in the elution barrel (1), separation holes (201) are annularly and equidistantly arranged in the raw material separation barrel (2), a bifunctional connecting part (3) is arranged between the outer bottom of the raw material separation barrel (2) and the inner bottom surface of the elution barrel (1), at least six groups of liquid inlet pipes (4) are annularly and equidistantly arranged at the position, close to the top, of the elution barrel (1), one end of each liquid inlet pipe (4) extends to the inner bottom surface of the elution barrel (1) and is not contacted with the inner bottom surface of the elution barrel (1), a preparation storage cavity (5) is arranged between the elution barrel (1) and the raw material separation barrel (2), and a pumping pipe (6) is inserted in the preparation storage cavity (5);
a lifting assembly (7) is arranged at the top of the raw material separation barrel (2);
the top of the elution barrel (1) is provided with a barrel cover (101), the upper surface of the barrel cover (101) is provided with a rotary driving assembly (8), and the rotary driving assembly (8) is in transmission connection with the raw material separation barrel (2);
a positioning shaft (202) is fixedly arranged on the bottom surface of the raw material separating cylinder (2), a linear bearing is fixedly arranged on the surface of the positioning shaft (202), a positioning pipe (102) is fixedly arranged on the inner bottom surface of the eluting cylinder (1), and the positioning shaft (202) is in sliding connection with the positioning pipe (102);
limiting plates (103) are symmetrically arranged on two sides of the cylinder cover (101), limiting grooves (104) are symmetrically formed on two sides of the elution cylinder (1), the limiting plates (103) and the limiting grooves (104) are in clearance fit, a limiting ring (105) is fixedly connected to the inner bottom surface of the cylinder cover (101), a turntable bearing (106) is fixedly arranged on the inner side of the limiting ring (105), the limiting ring (105) and the raw material separation cylinder (2) are in clearance fit, and rubber base plates (107) are arranged on the tops of the limiting ring (105) and the raw material separation cylinder (2);
the lifting assembly (7) comprises a support (701), the support (701) is arranged on one side of the elution barrel (1), two groups of electric push rods (702) are symmetrically arranged at the top of the support (701), a connecting joint (703) is fixedly arranged at the telescopic rod end of each electric push rod (702), and the connecting joint (703) is fixedly connected with the upper surface of the barrel cover (101).
2. The apparatus for producing a preparation for promoting the synthesis of gardenia crocin according to claim 1, wherein: the mouth of the liquid inlet pipe (4) is fixedly provided with a valve, the mouth of the liquid inlet pipe (4) is provided with a label, and the label corresponds to different types of preparations.
3. The apparatus for producing a preparation for promoting the synthesis of gardenia crocin according to claim 1, wherein: the rotary driving assembly (8) comprises a driving motor (801), the driving motor (801) is fixedly arranged on the upper surface of the cylinder cover (101), a linkage shaft (802) is fixedly arranged at the middle position in the raw material separating cylinder (2), synchronous pulleys are fixedly arranged at the top end of the linkage shaft (802) and the output shaft end of the driving motor (801), and synchronous belts are connected between the two groups of synchronous pulleys in a transmission mode.
4. The apparatus for producing a preparation for promoting the synthesis of gardenia crocin according to claim 1, wherein: the device is characterized in that a flowmeter (601) is arranged in the extraction tube (6), one end of the extraction tube (6) penetrates through the tube cover (101) and extends into the elution tube (1), a preparation collecting tube (9) is arranged on one side of the tube cover (101), and the other end of the extraction tube (6) extends into the preparation collecting tube (9).
5. A method of operating a device for producing a preparation for the synthesis of gardenia-promoted crocin according to any one of claims 1 to 4, comprising the steps of:
s1, loading a raw product containing a gardenia raw material extract into a raw material separating cylinder (2), respectively adding eluent of ethyl acetate, methanol and water into an eluting cylinder (1) through a liquid inlet pipe (4) attached with a label, and eluting the gardenia raw material, wherein the ethyl acetate: methanol: the proportion of water is 15-20:20-3.5:0.05-0.25, in the elution process, starting a driving motor (801) to drive a linkage shaft (802), so as to drive a raw material separating cylinder (2) to rotate, and detecting the crocin aglycone content in the gardenia through HPLC chromatography after sufficient elution;
s2, the eluting concentrated solution in the step S1 is left in an eluting cylinder (1), the gardenia raw material in a raw material separating cylinder (2) is taken out by utilizing a lifting component (7), the eluting concentrated solution is reserved, an acetonitrile water solution with the concentration of 15-22% is continuously poured into the eluting cylinder (1) through a liquid inlet pipe (4) for continuous eluting until a secondary concentrated solution is obtained, and the crocin aglycone content in the gardenia is detected by an HPLC (high performance liquid chromatography) method;
s3, taking out the secondary concentrated solution through a pumping pipe (6) to a preparation collecting cylinder (9), fusing the secondary concentrated solution with 40-60% methanol aqueous solution at the pressure of 5-15.0 Mpa, removing a small amount of front and rear impurities, and collecting the preparation for promoting the synthesis of the gardenia crocin.
6. The method for operating a production apparatus for a gardenia-promoting crocin pigment synthesis preparation according to claim 5, wherein: the eluting pressure in the eluting cylinder (1) used in the step S2 is 0.05-0.55 Mpa.
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| CN112915582A (en) * | 2021-01-25 | 2021-06-08 | 王凯捷 | Instrument is drawed to water-soluble natural pigment in red heart dragon fruit in lip glaze preparation |
| CN112940056A (en) * | 2021-02-03 | 2021-06-11 | 上海诗丹德标准技术服务有限公司 | Preparation method of crocin reference substance |
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| GB0813747D0 (en) * | 2007-07-26 | 2008-09-03 | Hoya Corp | Separation method |
| CN206872713U (en) * | 2017-06-24 | 2018-01-12 | 江苏连恒生物科技有限公司 | A kind of analysis extraction element of blackberry, blueberry anthocyanidin |
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