CN114870428A - Production device and working method of preparation for promoting synthesis of gardenia crocetin - Google Patents
Production device and working method of preparation for promoting synthesis of gardenia crocetin Download PDFInfo
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- CN114870428A CN114870428A CN202210368633.2A CN202210368633A CN114870428A CN 114870428 A CN114870428 A CN 114870428A CN 202210368633 A CN202210368633 A CN 202210368633A CN 114870428 A CN114870428 A CN 114870428A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 54
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 31
- 230000001737 promoting effect Effects 0.000 title claims abstract description 30
- 238000003786 synthesis reaction Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 13
- PANKHBYNKQNAHN-JTBLXSOISA-N Crocetin Natural products OC(=O)C(\C)=C/C=C/C(/C)=C\C=C\C=C(\C)/C=C/C=C(/C)C(O)=O PANKHBYNKQNAHN-JTBLXSOISA-N 0.000 title claims description 19
- PANKHBYNKQNAHN-JUMCEFIXSA-N carotenoid dicarboxylic acid Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C(=O)O)C=CC=C(/C)C(=O)O PANKHBYNKQNAHN-JUMCEFIXSA-N 0.000 title claims description 19
- PANKHBYNKQNAHN-MQQNZMFNSA-N crocetin Chemical compound OC(=O)C(/C)=C/C=C/C(/C)=C/C=C/C=C(\C)/C=C/C=C(\C)C(O)=O PANKHBYNKQNAHN-MQQNZMFNSA-N 0.000 title claims description 14
- 240000001972 Gardenia jasminoides Species 0.000 title 1
- 238000010828 elution Methods 0.000 claims abstract description 76
- 239000002994 raw material Substances 0.000 claims abstract description 59
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- 239000007788 liquid Substances 0.000 claims abstract description 15
- 238000003860 storage Methods 0.000 claims abstract description 9
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 18
- 244000124209 Crocus sativus Species 0.000 claims description 12
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- 238000004587 chromatography analysis Methods 0.000 claims description 7
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- 238000011068 loading method Methods 0.000 claims description 3
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 claims description 3
- 238000011017 operating method Methods 0.000 claims 2
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- SEBIKDIMAPSUBY-ARYZWOCPSA-N Crocin Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H](O)[C@@H]1O)O)OC(=O)C(C)=CC=CC(C)=C\C=C\C=C(/C)\C=C\C=C(C)C(=O)O[C@H]1[C@@H]([C@@H](O)[C@H](O)[C@@H](CO[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)O1)O)O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SEBIKDIMAPSUBY-ARYZWOCPSA-N 0.000 abstract description 7
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Images
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0215—Solid material in other stationary receptacles
- B01D11/0223—Moving bed of solid material
- B01D11/0238—Moving bed of solid material on fixed or rotating flat surfaces, e.g. tables combined with rotating elements or on rotating flat surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/0288—Applications, solvents
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Extraction Or Liquid Replacement (AREA)
- Saccharide Compounds (AREA)
Abstract
The invention discloses a production device of a preparation for promoting synthesis of gardenia crocein, which comprises an elution cylinder, wherein a raw material separation cylinder is arranged in the elution cylinder, an annular equidistant array in the raw material separation cylinder is provided with separation holes, a dual-function connecting part is arranged between the outer bottom of the raw material separation cylinder and the inner bottom surface of the elution cylinder, the annular equidistant array at the position close to the top of the elution cylinder is provided with at least six groups of liquid inlet pipes, one end of each liquid inlet pipe extends to the inner bottom surface of the elution cylinder and is not contacted with the inner bottom surface of the elution cylinder, a preparation storage cavity is arranged between the elution cylinder and the raw material separation cylinder, and a suction pipe is inserted in the preparation storage cavity; meanwhile, the invention also discloses a working method of the production device of the preparation for promoting the synthesis of the gardenia crocein, which is characterized in that after the endophyte with the synergistic promotion effect is used as the main material, the main material is compounded, the total preparation for promoting the synthesis of the gardenia crocein is found, and the extraction method of the crocin and the acquisition range of the carrier are enlarged.
Description
Technical Field
The invention relates to the technical field of crocin synthesizing agents, in particular to a production device and a working method of a preparation for promoting synthesis of gardenia crocin.
Background
Phosphorus, potassium, calcium, magnesium, iron, zinc, boron and the like are essential elements for the growth of plant fruits, promote the expansion and development of the fruits and uniformly color, and can directly supplement nutrient elements lacking in soil, activate beneficial microbial bacteria and inhibit harmful bacteria as a natural soil conditioner. For example, chitin: the activated cells have the effect of a plant vaccine, strengthen the resistance of fruit trees to adverse natural environment, induce and generate resistance factors to diseases, and thus can reduce the incidence of diseases; cyclopentane polyhydrophenanthrene: the plant growth regulator plays an important role in plant growth, participates in many aspects of plant development regulation and control, and plays an important role in plant defense against environmental stress; refined seaweed: the fertilizer is rich in 18 proteinogenic amino acids and algal polysaccharides which can be directly absorbed and utilized by plants, and can promote the formation of soil aggregate structures and improve the internal pore spaces of soil.
Crocus sativus is a plant of crocus of Iridaceae, native to southern Europe to Iran, introduced into Tibet through India, so it is also called saffron, which is commonly used and cultivated in Zhejiang, Jiangxi, Jiangsu, Beijing and Shanghai in China. The crocus sativus is a famous and precious medicinal material and a famous and precious spice, and modern pharmacological research shows that the crocus sativus has strong circulatory system effect and biological activities such as anti-tumor and anti-oxidation. According to the traditional Chinese medicine, the dry stigma is used as a medicine, namely saffron, and has the effects of promoting blood circulation to remove blood stasis, cooling blood and removing toxicity, and relieving depression and soothing nerves. Crocin, also known as crocin, is a generic term for carotenoids, crocin and other pigments extracted from stigma croci.
Because of the small amount of extraction carrier and the low content, a preparation for synthesizing crocetin and a working method of a preparation production device are needed.
Disclosure of Invention
The invention aims to provide a production device of a preparation for promoting synthesis of gardenia saffron pigment, which finds out a preparation total for promoting synthesis of gardenia saffron pigment, increases an extraction method of saffron pigment and an acquisition range of a carrier, and solves the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
the production device of the preparation for promoting the synthesis of the gardenia crocetin comprises an elution cylinder, wherein a raw material separation cylinder is arranged in the elution cylinder, separation holes are formed in an annular equidistant array in the raw material separation cylinder, a dual-function connecting part is arranged between the outer bottom of the raw material separation cylinder and the inner bottom surface of the elution cylinder, at least six groups of liquid inlet pipes are arranged in the annular equidistant array at the position, close to the top, of the elution cylinder, one end of each liquid inlet pipe extends to the inner bottom surface of the elution cylinder and is not in contact with the inner bottom surface of the elution cylinder, a preparation storage cavity is formed between the elution cylinder and the raw material separation cylinder, and a drawing-off pipe is inserted in the preparation storage cavity;
a lifting assembly is arranged at the top of the raw material separation cylinder;
the top of the elution barrel is provided with a barrel cover, the upper surface of the barrel cover is provided with a rotary driving assembly, and the rotary driving assembly is in transmission connection with the raw material separation barrel.
The bottom surface of the raw material separation barrel is fixedly provided with a positioning shaft, the surface of the positioning shaft is fixedly provided with a linear bearing, the inner bottom surface of the elution barrel is fixedly provided with a positioning pipe, and the positioning shaft is inserted in the positioning pipe in a sliding manner.
Wherein, the equal fixed mounting in mouth of pipe of feed liquor pipe has the valve, and the mouth of pipe of feed liquor pipe is provided with the sign subsides, the sign pastes the preparation that corresponds different classes.
The device comprises a barrel cover, a washing barrel, a limiting plate, a limiting groove, a limiting ring, a rotating disc bearing, a limiting ring, a raw material separating barrel and a rubber base plate, wherein the limiting plate is symmetrically arranged on two sides of the barrel cover, the limiting groove is symmetrically arranged on two sides of the washing barrel, the limiting plate and the limiting groove are in clearance fit, the limiting ring is fixedly connected to the inner bottom surface of the barrel cover, the rotating disc bearing is fixedly arranged on the inner side of the limiting ring, the limiting ring and the raw material separating barrel are in clearance fit, and the rubber base plate is arranged at the tops of the limiting ring and the raw material separating barrel.
The lifting assembly comprises a support, the support is arranged on one side of the elution barrel, two groups of electric push rods are symmetrically arranged at the top of the support, a connecting joint is fixedly mounted at the end of a telescopic rod of each electric push rod, and the connecting joint is fixedly connected with the upper surface of the barrel cover.
The rotary driving assembly comprises a driving motor, the driving motor is fixedly installed on the upper surface of the barrel cover, a linkage shaft is fixedly installed at the middle position in the raw material separating barrel, synchronous belt wheels are fixedly installed at the top ends of the linkage shaft and the output shaft ends of the driving motor, and a synchronous belt is connected between the two groups of synchronous belt wheels in a transmission mode.
Wherein, take out and be provided with the flowmeter in the pipe, take out and run through in the cover and extend to the elution section of thick bamboo from pipe one end, cover one side is provided with the preparation collecting vessel, take out and extend to in the preparation collecting vessel from the other end of pipe.
The invention also provides a technical scheme, and a working method of the production device for promoting the synthesis of the preparation for the gardenia crocetin comprises the following steps:
s1, loading the crude product containing the gardenia raw material extract into a raw material separation cylinder, adding ethyl acetate, methanol and water eluent into an elution cylinder through a liquid inlet pipe attached with an identification sticker, and eluting the gardenia raw material, wherein the ethyl acetate: methanol: the proportion of water is 15-20: 20-3.5: 0.05-0.25, in the elution process, starting a driving motor to drive a linkage shaft so as to drive a raw material separation barrel to rotate, and detecting the content of crocetin glycoside in the gardenia by HPLC chromatography after sufficient elution is obtained;
s2, reserving the elution concentrated solution obtained in the step S1 in an elution cylinder, taking out the gardenia raw material in the raw material separation cylinder by using a lifting assembly, reserving the elution concentrated solution, continuously filling acetonitrile water solution with the concentration of 15-22% into the elution cylinder through a liquid inlet pipe, continuously eluting until a secondary concentrated solution is obtained, and detecting the crocin glycoside content in the gardenia through HPLC chromatography;
s3, taking out the secondary concentrated solution to a preparation collecting cylinder through a drawing tube, fusing the secondary concentrated solution with 40-60% methanol water solution under the pressure of 5-15.0 Mpa, removing a small amount of impurities in front and back, and collecting the preparation for promoting the synthesis of the gardenia crocein.
Wherein the elution pressure in the elution cylinder used in the step S2 is 0.05-0.55 MPa.
In summary, due to the adoption of the technology, the invention has the beneficial effects that:
in the invention, the content of crocin glucoside in gardenia is detected by HPLC chromatography: 13.21g/100 g; meanwhile, the gardenia endophyte with the saffron glycoside biosynthesis effect is screened, the two-strain combination is carried out on the gardenia endophyte, the endophyte with the synergistic promotion effect is used as the main material for compounding, the total preparation for promoting the synthesis of the gardenia saffron pigment is found, and the extraction method of the saffron pigment and the acquisition range of the carrier are enlarged;
according to the invention, the elution barrel and the raw material separation barrel are sequentially arranged, the raw material separation barrel is rotationally driven by the driving assembly, so that the gardenia raw material and the preparation are better and fully fused for elution, and meanwhile, the lifting assembly is arranged to rapidly take out the raw material separation barrel, so that the raw material is separated, the addition and reaction of a secondary elution preparation are ensured, the elution efficiency is improved, and the crocetin is rapidly obtained.
Drawings
FIG. 1 is a schematic perspective view of a production apparatus for producing a preparation for promoting the synthesis of gardenia crocein according to the present invention;
FIG. 2 is a schematic view showing a sectional three-dimensional structure of an elution column of the apparatus for producing a preparation for promoting the synthesis of gardenia crocein according to the present invention;
FIG. 3 is a schematic perspective view showing the bottom surface mounting structure of the cartridge cover of the apparatus for producing a preparation for promoting the synthesis of gardenia crocein according to the present invention;
FIG. 4 is a flowchart of the apparatus for producing a preparation for promoting the synthesis of gardenia crocetin of the present invention.
In the figure: 1. an elution cartridge; 101. a cylinder cover; 102. a positioning tube; 103. a limiting plate; 104. a limiting groove; 105. a limiting ring; 106. a turntable bearing; 107. a rubber pad; 2. a raw material separation cylinder; 201. a separation well; 202. positioning the shaft; 3. a dual function connection portion; 4. a liquid inlet pipe; 5. a formulation storage cavity; 6. drawing the tube; 601. a flow meter; 7. a lifting assembly; 701. a support; 702. an electric push rod; 703. connecting a joint; 8. a rotary drive assembly; 801. a drive motor; 802. a linkage shaft; 9. a preparation collecting cylinder.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the description of the present invention, it is to be understood that the terms indicating an orientation or positional relationship are based on the orientation or positional relationship shown in the drawings only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art from the specification.
Example 1
The invention provides a production device of a preparation for promoting gardenia crocein synthesis as shown in figures 1-3, which comprises an elution cylinder 1, wherein a raw material separation cylinder 2 is arranged in the elution cylinder 1, separation holes 201 are arranged in an annular equidistant array in the raw material separation cylinder 2, a dual-function connecting part 3 is arranged between the outer bottom of the raw material separation cylinder 2 and the inner bottom surface of the elution cylinder 1, at least six groups of liquid inlet pipes 4 are arranged in the annular equidistant array at the position close to the top of the elution cylinder 1, one end of each liquid inlet pipe 4 extends to the inner bottom surface of the elution cylinder 1 and is not contacted with the inner bottom surface of the elution cylinder 1, a preparation storage cavity 5 is arranged between the elution cylinder 1 and the raw material separation cylinder 2, a drawing pipe 6 is inserted in the preparation storage cavity 5, a flow meter 601 is arranged in the drawing pipe 6, one end of the drawing pipe 6 penetrates through a cylinder cover 101 and extends into the elution cylinder 1, a preparation collecting cylinder 9 is arranged at one side of the cylinder cover 101, the other end of the drawing-off tube 6 extends into the preparation collecting cylinder 9.
The bottom surface of the raw material separation barrel 2 is fixedly provided with a positioning shaft 202, the surface of the positioning shaft 202 is fixedly provided with a linear bearing, the inner bottom surface of the elution barrel 1 is fixedly provided with a positioning tube 102, and the positioning shaft 202 is inserted in the positioning tube 102 in a sliding manner.
The equal fixed mounting in mouth of pipe of feed liquor pipe 4 has the valve, and the mouth of pipe of feed liquor pipe 4 is provided with the sign subsides, the sign pastes the preparation that corresponds different classes.
The cylinder cover 101 bilateral symmetry is provided with limiting plate 103, the spacing groove 104 has been seted up to an elution section of thick bamboo 1 bilateral symmetry, limiting plate 103 and spacing groove 104 clearance fit, bottom surface fixedly connected with spacing ring 105 in the cylinder cover 101, the inboard fixed mounting of spacing ring 105 has a slewing bearing 106, spacing ring 105 and 2 clearance fits of raw material separating cylinder, and spacing ring 105 and 2 tops of raw material separating cylinder are provided with rubber tie plate 107.
The top of the raw material separation barrel 2 is provided with a lifting assembly 7, the lifting assembly 7 comprises a support 701, the support 701 is arranged on one side of the elution barrel 1, two sets of electric push rods 702 are symmetrically arranged on the top of the support 701, a connecting joint 703 is fixedly installed at the telescopic rod end of each electric push rod 702, and the connecting joint 703 is fixedly connected with the upper surface of the barrel cover 101.
The top of the elution barrel 1 is provided with a barrel cover 101, the upper surface of the barrel cover 101 is provided with a rotary driving assembly 8, the rotary driving assembly 8 is connected with the transmission of the raw material separation barrel 2, the rotary driving assembly 8 comprises a driving motor 801, the driving motor 801 is fixedly installed on the upper surface of the barrel cover 101, a linkage shaft 802 is fixedly installed at the center of the raw material separation barrel 2, synchronous belt wheels are fixedly installed at the top ends of the linkage shaft 802 and the output shaft ends of the driving motor 801, and a synchronous belt is connected between the two groups of synchronous belt wheels in a transmission manner.
As shown in figure 4, the working method of the production device of the preparation for promoting the synthesis of gardenia crocein comprises the following steps:
s1, loading the crude product containing the gardenia raw material extract into a raw material separation cylinder 2, adding ethyl acetate, methanol and water eluent into an elution cylinder 1 through a liquid inlet pipe 4 attached with an identification sticker, and eluting the gardenia raw material, wherein the ethyl acetate: methanol: the proportion of water is 15-20: 20-3.5: 0.05-0.25, in the elution process, starting a driving motor 801 to drive a linkage shaft 802, so as to drive a raw material separation barrel 2 to rotate, and detecting the crocetin glycoside content in the gardenia by HPLC chromatography after sufficient elution is obtained;
s2, reserving the elution concentrated solution obtained in the step S1 in the elution cylinder 1, taking out the gardenia raw material in the raw material separation cylinder 2 by using the lifting assembly 7, reserving the elution concentrated solution, continuously filling acetonitrile water solution with the concentration of 15-22% into the elution cylinder 1 through the liquid inlet pipe 4, continuously eluting until a secondary concentrated solution is obtained, and detecting the crocetin glycoside content in the gardenia through HPLC chromatography;
s3, taking out the secondary concentrated solution through a drawing tube 6 to a preparation collecting cylinder 9, fusing the secondary concentrated solution with 40-60% methanol water solution under the pressure of 5-15.0 Mpa, removing a small amount of impurities in front and back, and collecting the preparation for promoting the synthesis of the gardenia crocein.
Specifically, the elution pressure in the elution cylinder 1 used in the step S2 is 0.05 to 0.55 Mpa.
Example 2
Pouring the melted culture medium into a sterile plate by adopting a plate coating method to prepare a sterile plate, cooling and solidifying, selecting the gardenia endophytic bacteria strain for culturing, dropwise adding a certain amount of strain suspension on the surface of the plate, uniformly dispersing the bacteria strain to the whole surface of the plate by using a sterile glass coating rod, culturing, and separating and purifying various strains;
performing gene sequencing on the strain
Extracting DNA of the endophyte, formulating quality control indexes, adopting a transposase method to segment the DNA and connect the barcodes after meeting the standard, mixing all libraries in proportion, connecting a sequencing joint, and finally accurately quantifying the DNA library by using the Qubit. After library construction was complete, a concentration and volume of DNA library was added to each individual Flow cell and the Flow cells were transferred to a gridios X5 sequencer for real-time single molecule sequencing.
Strains which regulate crocin glycosidyl genes are selected by gene sequencing and comparative genomic analysis. Thereafter, the growth curves of these several strains in gardenia were dynamically monitored. After strain 1 dies down, the number of strain 2 is rapidly reduced through a curve, and then the two strains are found to have a synergistic relationship after being researched, so that a design experiment is obtained, a single strain and double strains are combined in a gardenia plant for experiment, the result is judged by measuring the quality of gardenia fruits and the content of a pigment glycoside medium, after the strain is determined, the bacterial colony is selected and inoculated in a new LB liquid culture medium for shake flask culture, and the amplification culture is carried out.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Claims (9)
1. The production device of the preparation for promoting the synthesis of the gardenia crocein is characterized by comprising an elution cylinder (1), wherein a raw material separation cylinder (2) is arranged in the elution cylinder (1), separation holes (201) are formed in the raw material separation cylinder (2) in an annular equidistant array, a dual-function connecting part (3) is arranged between the outer bottom of the raw material separation cylinder (2) and the inner bottom surface of the elution cylinder (1), at least six groups of liquid inlet pipes (4) are arranged in the elution cylinder (1) close to the top in an annular equidistant array, one end of each liquid inlet pipe (4) extends to the inner bottom surface of the elution cylinder (1) and is not contacted with the inner bottom surface of the elution cylinder (1), a preparation storage cavity (5) is formed between the elution cylinder (1) and the raw material separation cylinder (2), and a drawing-off pipe (6) is inserted in the preparation storage cavity (5);
a lifting component (7) is arranged at the top of the raw material separation cylinder (2);
a barrel cover (101) is arranged at the top of the elution barrel (1), a rotary driving assembly (8) is arranged on the upper surface of the barrel cover (101), and the rotary driving assembly (8) is in transmission connection with the raw material separation barrel (2).
2. The apparatus for producing a preparation for promoting the synthesis of gardenia crocetin according to claim 1, characterized in that: the bottom surface of the raw material separation barrel (2) is fixedly provided with a positioning shaft (202), the surface of the positioning shaft (202) is fixedly provided with a linear bearing, the inner bottom surface of the elution barrel (1) is fixedly provided with a positioning pipe (102), and the positioning shaft (202) is inserted into the positioning pipe (102) in a sliding manner.
3. The apparatus for producing a preparation for promoting the synthesis of gardenia crocetin according to claim 1, characterized in that: the equal fixed mounting in mouth of pipe of feed liquor pipe (4) has the valve, and the mouth of pipe of feed liquor pipe (4) is provided with the sign subsides, the sign pastes the preparation that corresponds different classes.
4. The apparatus for producing a preparation for promoting the synthesis of gardenia crocetin according to claim 1, characterized in that: cover (101) bilateral symmetry is provided with limiting plate (103), spacing groove (104) have been seted up to an elution section of thick bamboo (1) bilateral symmetry, limiting plate (103) and spacing groove (104) clearance fit, bottom surface fixedly connected with spacing ring (105) in cover (101), spacing ring (105) inboard fixed mounting has slewing bearing (106), spacing ring (105) and raw material separating cylinder (2) clearance fit, and spacing ring (105) and raw material separating cylinder (2) top are provided with rubber backing plate (107).
5. The apparatus for producing a preparation for promoting the synthesis of gardenia crocetin according to claim 1, characterized in that: the lifting assembly (7) comprises a support (701), the support (701) is arranged on one side of the elution barrel (1), two sets of electric push rods (702) are symmetrically arranged at the top of the support (701), a connecting joint (703) is fixedly installed at the telescopic rod end of each electric push rod (702), and the connecting joint (703) is fixedly connected with the upper surface of the barrel cover (101).
6. The apparatus for producing a preparation for promoting the synthesis of gardenia crocetin according to claim 1, characterized in that: rotatory drive assembly (8) include driving motor (801), driving motor (801) fixed mounting is in cover (101) upper surface, position fixed mounting placed in the middle has universal driving shaft (802) in raw materials separating cylinder (2), the equal fixed mounting of output axle head on universal driving shaft (802) top and driving motor (801) has synchronous pulley, and the transmission is connected with the hold-in range between two sets of synchronous pulley.
7. The apparatus for producing a preparation for promoting the synthesis of gardenia safflorin according to claim 1, wherein: be provided with flowmeter (601) in drawing from pipe (6), draw from pipe (6) one end and run through in cover (101) and extend to elution section of thick bamboo (1), cover (101) one side is provided with preparation collecting cylinder (9), draw from the other end of pipe (6) and extend to in preparation collecting cylinder (9).
8. An operating method of the apparatus for producing a preparation for promoting synthesis of gardenia crocetin according to claims 1 to 7, characterized by comprising the steps of:
s1, loading the crude product containing the gardenia raw material extract into a raw material separation cylinder (2), adding the eluent of ethyl acetate, methanol and water into an elution cylinder (1) through a liquid inlet pipe (4) attached with an identification sticker, and eluting the gardenia raw material, wherein the ethyl acetate: methanol: the proportion of water is 15-20: 20-3.5: 0.05-0.25, in the elution process, starting a driving motor (801) to drive a linkage shaft (802), so as to drive a raw material separation barrel (2) to rotate, and detecting the content of crocetin glycoside in the gardenia by HPLC chromatography after sufficient elution is obtained;
s2, reserving the elution concentrated solution obtained in the step S1 in an elution cylinder (1), taking out the gardenia raw material in the raw material separation cylinder (2) by using a lifting assembly (7), reserving the elution concentrated solution, continuously filling acetonitrile water solution with the concentration of 15-22% into the elution cylinder (1) through a liquid inlet pipe (4) for continuous elution until a secondary concentrated solution is obtained, and detecting the content of crocetin glycoside in the gardenia by using HPLC chromatography;
s3, taking out the secondary concentrated solution through a drawing tube (6) to a preparation collecting cylinder (9), fusing the secondary concentrated solution with 40-60% methanol water solution under the pressure of 5-15.0 Mpa, removing a small amount of front and rear impurities, and collecting the preparation for promoting the synthesis of the gardenia saffron pigment.
9. The operating method of the apparatus for producing a preparation for promoting synthesis of gardenia crocetin according to claim 8, characterized in that: the elution pressure in the elution cylinder (1) used in the step S2 is 0.05-0.55 Mpa.
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| CN112915582A (en) * | 2021-01-25 | 2021-06-08 | 王凯捷 | Instrument is drawed to water-soluble natural pigment in red heart dragon fruit in lip glaze preparation |
| CN112940056A (en) * | 2021-02-03 | 2021-06-11 | 上海诗丹德标准技术服务有限公司 | Preparation method of crocin reference substance |
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| GB0813747D0 (en) * | 2007-07-26 | 2008-09-03 | Hoya Corp | Separation method |
| CN206872713U (en) * | 2017-06-24 | 2018-01-12 | 江苏连恒生物科技有限公司 | A kind of analysis extraction element of blackberry, blueberry anthocyanidin |
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