CN211978938U - Kit for rapidly detecting melamine in food - Google Patents

Abstract

The utility model relates to a kit for short-term test melamine in food, including the box body and with box body one end articulated lid, set up the rectangular channel that is linked together with the external world in the box body, be provided with in the rectangular channel and place the board, the upper surface of placing the board is opened there is a plurality of to place the chamber, every bottom surface of placing the chamber all is equipped with a plurality of reagent storage tank, places the board top and is equipped with the mount, opening below the mount, the mount lock is on placing the board, the upper surface of mount is opened there is the low groove, has placed the ELIAS board in the low groove. The utility model discloses an indirect competition ELISA method detects melamine content, and detection cost is lower, has quick simple and convenient, sensitive accurate detection advantage, and structural design is reasonable in the reagent box moreover, makes things convenient for the staff to use, and built-in thermal-insulated heat preservation avoids external environment to cause the influence to the reagent in the reagent box.

Description

Kit for rapidly detecting melamine in food

Technical Field

The utility model relates to a kit especially relates to a kit that is arranged in melamine of short-term test food, belongs to food detection technology field.

Background

Melamine (melamine) is a pure white monoclinic prismatic crystal with low toxicity and no smell, is similar to protein powder, belongs to triazine nitrogen-containing heterocyclic ring organic compounds, and is widely applied to the production of plastics, dyes and chemical fertilizers and the textile industry. The nitrogen content of the melamine is up to 66.6%, and the melamine can cause a false image with higher protein content in detection only by adding the melamine into food, so that serious food safety accidents such as US pet food pollution events in 2007 and Chinese poisonous milk powder events in 2008 are caused, and the melamine residue phenomenon is found in related products such as feed, milk powder, biscuits, cream candy and the like at present. Animal experiment results show that the intake of melamine can cause reproduction disorder, cystitis, chronic nephritis, kidney stone and even bladder cancer, so that the rapid detection of melamine components in biomedical samples, particularly foods, has very important significance.

At present, the domestic method for detecting melamine is mainly high performance liquid chromatography, and the confirmation method is a gas mass spectrometry combined method, but the method is complex, has high detection cost, and wastes a large amount of manpower and material resources; enzyme-linked immunosorbent assay (ELISA) has the advantages of high specificity, high sensitivity, good stability and simple operation, can simultaneously and rapidly detect a large number of samples, and is widely used for primary screening of veterinary drugs and toxin detection in food at present. In order to make up for the defects of various detection methods, various melamine detection kits are developed in succession, and the principle of applying an enzyme-linked immunosorbent assay (ELISA) is mainly focused, but the melamine kit on the market at present has a simple structure and no corresponding heat preservation structure, and the built-in reagent bottles are randomly stacked and are easy to be damaged in the transportation or storage process.

SUMMERY OF THE UTILITY MODEL

The utility model discloses mainly be to the not good, the unreasonable problem of structural design of melamine kit heat preservation effect among the prior art, provide a kit for melamine in short-term test food, this kit simple structure adopts indirect competition ELISA method to detect melamine content, and the detection cost is lower, has quick simple and convenient, sensitive accurate detection advantage, and structural design is reasonable in the kit in addition, makes things convenient for the staff to use, and built-in thermal-insulated heat preservation avoids external environment to cause the influence to reagent in the kit.

The purpose of the utility model is mainly realized by the following scheme:

the utility model provides a kit for melamine in short-term test food, including the box body and with box body one end articulated lid, set up the rectangular channel that is linked together with the external world in the box body, be provided with in the rectangular channel and place the board, the upper surface of placing the board is opened there is a plurality of to place the chamber, every the bottom surface of placing the chamber all is equipped with a plurality of reagent storage tank, it is equipped with the mount to place the board top, opening below the mount, the mount lock is on placing the board, the upper surface of mount is opened there is the low groove, the ELIAS board has been placed in the low groove, and the reagent bottle is placed in the reagent storage tank, and inner structure reasonable in design makes the reagent bottle put neatly, and the staff of being convenient for uses, also can avoid transporting or deposit the in-.

Preferably, it is equipped with 3 to place the chamber, reagent storage tank is equipped with 14, 3 place among the chamber wherein 2 place the intracavity and all be equipped with 4 reagent storage tanks, another place the intracavity and be equipped with 6 reagent storage tanks, the reagent bottle is placed rationally, can place the operation of being convenient for according to use order or use custom.

Preferably, the reagent storage tank stores reagent bottles, and the reagents contained in 14 reagent bottles are: 6 bottles of standard substance solution, 1 bottle of high-concentration standard substance solution, 1 bottle of melamine enzyme marker, 1 bottle of melamine anti-reagent, 1 bottle of substrate liquid A liquid, 1 bottle of substrate liquid B liquid, 1 bottle of stop solution, 1 bottle of concentrated cleaning solution and 1 bottle of melamine concentrated sample diluent, adopt indirect competition ELISA method to detect melamine content, detection cost is lower, has quick simple and convenient, sensitive accurate detection advantage moreover.

Preferably, the elisa plate is a 96-hole polystyrene elisa plate, and the elisa plate is placed in a vacuum aluminum foil bag, so that the elisa plate can be used for detecting a plurality of continuous samples at one time, and can also be used for detecting a plurality of times by disassembling the plate holes.

Preferably, the fixing frame is internally provided with fixed foam which is abutted against the upper surface of the reagent bottle, so that the reagent bottle is prevented from shaking to cause damage in transportation or placement.

Preferably, the upper surface of the placing plate is pasted with a label at the position corresponding to the reagent bottle, so that the staff can conveniently identify the type of the reagent contained in the reagent bottle, and the operation is convenient.

Preferably, the front surface of box body is provided with the buckle, the front side of lid be provided with the splint that bayonet socket cooperation was used, buckle and splint cooperation are used, the switching of the kit of being convenient for.

Preferably, the upper surfaces of the placing plate and the fixing frame are internally provided with two mutually symmetrical holding parts, the placing plate and the fixing frame are placed through the holding parts, the design is reasonable, and the operation is convenient.

As the preferred, all be equipped with thermal-insulated heat preservation on the inner wall of box body and lid, increased insulation construction, reduced the influence of external environment to reagent.

Preferably, a pressing plate is arranged on the heat insulation layer on the inner wall of the box cover, when the box cover is closed, the pressing plate covers the lower groove, the pressing plate can compact the interior of the box body, and the phenomenon that the box body is shaken due to gaps between the pressing plate and the box body, between the pressing plate and the fixing frame, between the fixing frame and the ELISA plate and between the ELISA plate and the box cover is avoided.

Therefore, the utility model discloses possess following advantage:

(1) the kit of the utility model adopts an indirect competition ELISA method to detect the content of melamine in food, has lower detection cost and has the advantages of rapidness, convenience, sensitivity and accuracy;

(2) the reagent kit has reasonable internal structural design, the reagent bottles are placed neatly, the reagent kit is convenient for workers to use, and the reagent bottles can be prevented from being damaged in the transportation or storage process;

(3) the utility model discloses the box body of kit and the inner wall of lid all are equipped with thermal-insulated heat preservation, can reduce the influence of external environment to reagent in the reagent bottle.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the box body and the box cover of the present invention;

FIG. 3 is a schematic structural view of the placing board of the present invention;

FIG. 4 is a schematic structural view of the fixing frame of the present invention taken out;

FIG. 5 is a schematic structural view of the placing board of the present invention when it is fastened to the fixing frame;

fig. 6 is a schematic structural view of the utility model when the box cover is closed.

Illustration of the drawings: 1-box body, 2-box cover, 3-rectangular groove, 4-placing plate, 5-placing cavity, 6-reagent storage groove, 7-fixing frame, 8-lower groove, 9-enzyme label plate, 10-label, 11-buckle, 12-clamp plate, 13-handle part and 14-pressing plate.

Detailed Description

The technical solution of the present invention is further specifically described below by way of examples and with reference to the accompanying drawings.

Example 1

As shown in fig. 1 and 6, the utility model provides a technical scheme, a kit for melamine in short-term test food, including box body 1 and with 1 one end articulated lid 2 of box body, as shown in fig. 2, set up the rectangular channel 3 that is linked together with the external world in the box body 1, be provided with in the rectangular channel 3 and place board 4, as shown in fig. 3, the upper surface of placing board 4 is opened has a plurality of to place chamber 5, every the bottom surface of placing chamber 5 all is equipped with a plurality of reagent storage tank 6, as shown in fig. 4, 5, places board 4 top and is equipped with mount 7, open under the mount 7, mount 7 lock is on placing board 4, and the upper surface of mount 7 is opened has low groove 8, the ELIAS plate 9 has been placed in the low groove 8.

Example 2

As shown in fig. 1 and 6, a kit for rapidly detecting melamine in food comprises a box body 1 and a box cover 2 hinged to one end of the box body 1, the materials of the box body 1 and the box cover 2 have the characteristics of light resistance and heat resistance, preferably plastics, particularly polyethylene, the color is white, the reflected light has low heat absorption coefficient, a buckle 11 is pasted on the front surface of the box body 1, a clamping plate 12 matched with the bayonet is pasted on the front side of the box cover 2, heat insulation layers are respectively arranged on the inner walls of the box body 1 and the box cover 2, the heat insulation layers of the embodiment adopt heat insulation coatings, as shown in fig. 2, a rectangular groove 3 communicated with the outside is arranged in the box body 1, a placing plate 4 made of hard plastics is arranged in the rectangular groove 3, as shown in fig. 3, a plurality of placing cavities 5 are arranged on the upper surface of the placing plate 4, and a plurality of reagent storage grooves 6 are arranged on the bottom surface of each placing, the upper surface of the placing plate 4 is adhered with labels 10 corresponding to the positions of the reagent bottles, in this embodiment, 3 placing cavities 5 are provided, 14 reagent storage grooves 6 are provided, 4 reagent storage grooves 6 are provided in 2 placing cavities 5 on the left side of the 3 placing cavities 5, 6 reagent storage grooves 6 are provided in the placing cavities 5 on the right side, as shown in fig. 4 and 5, a fixing frame 7 made of hard plastic is provided above the placing plate 4, the lower end of the fixing frame 7 is open, the fixing frame 7 is buckled on the placing plate 4, fixed foam is provided inside the fixing frame 7, the fixed foam is abutted against the upper surface of the reagent bottles, a lower groove 8 is provided on the upper surface of the fixing frame 7, an elisa plate 9 is provided in the lower groove 8, two mutually symmetrical handle parts 13 are provided in the upper surfaces of the placing plate 4 and the fixing frame 7, a pressing plate 14 is provided on the heat insulating layer on the inner wall of the box cover 2, when the box, the pressing plate 14 covers the lower groove 8, and the pressing plate 14 is made of soft silica gel.

Reagent storage tank 6 is internal to be deposited with the reagent bottle, and 14 reagent bottles are built-in the reagent be: 6 bottles of standard substance solution, 1ml and 1 bottle of high-concentration standard substance solution, 1ml and 1 bottle of melamine enzyme marker, 6ml and 1 bottle of melamine anti-reagent, 6ml and 1 bottle of substrate solution A, 6ml and 1 bottle of substrate solution B, 6ml and 1 bottle of stop solution, 6ml and 1 bottle of concentrated cleaning solution, 40ml and 1 bottle of melamine concentrated sample diluent and 12 ml; the ELISA plate 9 adopts a 96-hole polystyrene ELISA plate 9, the ELISA plate 9 is placed in a vacuum aluminum foil bag, the ELISA plate 9 adopts a 96-hole reagent plate as a solid phase carrier, the reagent plate consists of 12 detachable ELISA reaction microporous strips, each detachable ELISA reaction microporous strip has 8 reaction holes, and melamine coupling antigen is pre-coated in the reaction holes.

It should be understood that this example is for illustrative purposes only and is not intended to limit the scope of the present invention. Furthermore, it should be understood that various changes and modifications of the present invention may be made by those skilled in the art after reading the teachings of the present invention, and these equivalents also fall within the scope of the appended claims.

Claims (10)

1. The utility model provides a kit for melamine in short-term test food, including box body (1) and with box cover (2) that box body (1) one end is articulated, its characterized in that: rectangular channel (3) that are linked together with the external world have been seted up in box body (1), be provided with in rectangular channel (3) and place board (4), the upper surface of placing board (4) is opened has a plurality of to place chamber (5), every the bottom surface of placing chamber (5) all is equipped with a plurality of reagent storage tank (6), it is equipped with mount (7) to place board (4) top, open below mount (7), mount (7) lock is on placing board (4), the upper surface of mount (7) is opened has low groove (8), ELIAS plate (9) have been placed in low groove (8).

2. The kit for rapidly detecting melamine in food according to claim 1, wherein: it is equipped with 3 to place chamber (5), reagent storage tank (6) are equipped with 14, 3 place in chamber (5) wherein 2 place chamber (5) in all be equipped with 4 reagent storage tank (6), another place and be equipped with 6 reagent storage tank (6) in chamber (5).

3. The kit for rapidly detecting melamine in food according to claim 2, wherein: reagent bottles are stored in the reagent storage tank (6), and reagents contained in 14 reagent bottles are respectively as follows: 6 bottles of standard substance solution, 1 bottle of high-concentration standard substance solution, 1 bottle of melamine enzyme marker, 1 bottle of melamine anti-reagent, 1 bottle of substrate solution A, 1 bottle of substrate solution B, 1 bottle of stop solution, 1 bottle of concentrated cleaning solution and 1 bottle of melamine concentrated sample diluent.

4. The kit for rapidly detecting melamine in food according to claim 3, wherein: the ELISA plate (9) adopts a 96-hole polystyrene ELISA plate (9), and the ELISA plate (9) is placed in a vacuum aluminum foil bag.

5. The kit for rapidly detecting melamine in food according to claim 4, wherein: fixed foam is arranged inside the fixing frame (7), and the fixed foam is abutted to the upper surface of the reagent bottle.

6. The kit for rapidly detecting melamine in food according to claim 5, wherein: the upper surface of the placing plate (4) is pasted with a label (10) at the position corresponding to the reagent bottle.

7. The kit for rapidly detecting melamine in food according to claim 1, wherein: the front surface of box body (1) is provided with buckle (11), the front side of lid (2) be provided with splint (12) that bayonet socket cooperation was used.

8. The kit for rapidly detecting melamine in food according to claim 1, wherein: two mutually symmetrical holding hand parts (13) are arranged on the upper surfaces of the placing plate (4) and the fixing frame (7).

9. The kit for rapidly detecting melamine in food according to claim 1, wherein: and heat insulation layers are arranged on the inner walls of the box body (1) and the box cover (2).

10. The kit for rapidly detecting melamine in food according to claim 9, wherein: and a pressing plate (14) is arranged on the heat insulation layer on the inner wall of the box cover (2), and when the box cover (2) is closed, the pressing plate (14) covers the lower groove (8).

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