CN214794828U

CN214794828U - ELISA kit for detecting ciprofloxacin

Info

Publication number: CN214794828U
Application number: CN202120581502.3U
Authority: CN
Inventors: 卢宝驹
Original assignee: Beijing Aoben Biotechnology Co ltd
Current assignee: Beijing Aoben Biotechnology Co ltd
Priority date: 2021-03-22
Filing date: 2021-03-22
Publication date: 2021-11-19
Anticipated expiration: 2031-03-22

Abstract

The utility model provides an ELISA kit for detecting ciprofloxacin, which comprises a box body and a box cover, wherein the box body is divided into a detection area and a storage area from top to bottom, two groups of ELISA plate positioning assemblies are arranged in the detection area, and a first storage drawer and a second storage drawer are slidably connected in the storage area; a first buffer storage plate is clamped and fixed in the first storage drawer, and a ciprofloxacin standard solution and a ciprofloxacin antibody are clamped in the first buffer storage plate; the second storage drawer is internally provided with a second buffer storage plate, the second buffer storage plate is internally provided with an enzyme-labeled secondary antibody, a stop solution, a substrate developing solution, a cleaning solution and a complex solution, and the second buffer storage plate is also internally provided with an enzyme-labeled plate and a specification. The reagent kit of the utility model has simple structure, reasonable design and design in different areas; the detection area is convenient for horizontally placing the enzyme label plate during detection; deposit the district in through first buffering and deposit board and second buffering and deposit the board and deposit various reagents, be convenient for get and put, can prevent to collide each other and lead to damaging simultaneously.

Description

ELISA kit for detecting ciprofloxacin

Technical Field

The utility model belongs to the technical field of ciprofloxacin detects, especially, relate to an ELISA kit for detecting ciprofloxacin.

Background

Ciprofloxacin (CIP), also known as ciprofloxacin, belongs to the third-generation quinolone antibacterial synthetic drugs, has a wide antibacterial spectrum and strong antibacterial activity, is one of the most antibacterial drugs in fluoroquinolone drugs clinically applied at present, and has been widely applied to human clinical and animal husbandry breeding industries. Because the medicine is easy to induce bacterial drug resistance, accumulation poisoning can occur after long-term application, so that articular cartilage diseases, photoreaction, hepatotoxicity and hepatotoxicity can be caused, genotoxicity which causes lymphocyte chromosomal variation can be caused, and human tendon rupture can be caused. As a common medicine for people and livestock, the medicine residue has greater harm to the health of human bodies through a food chain, and is not beneficial to the treatment of human diseases by the medicine. Therefore, it is necessary to enhance the residual detection and supervision of the drug in animal food. European union, united states. The highest residual quantity of ciprofloxacin in animal-derived foods is forbidden or limited in Japan, China and the like.

At present, the main methods for detecting ciprofloxacin residues in animal derived foods comprise a microbiological method, a fluorescence method, a High Performance Liquid Chromatography (HPLC), a liquid chromatography-mass spectrometry (LC-MS), a capillary electrophoresis method, an enzyme-linked immunosorbent assay (ELISA) and the like. Commonly used methods for detecting ciprofloxacin are chromatographic and immunological detection methods. The chromatographic detection method is sensitive and accurate, needs expensive instruments and special workers, is relatively complicated in sample treatment, and has certain limitation on application, especially in basic large-scale screening and field detection. The immunological detection method is sensitive, specific, rapid, simple and convenient, overcomes the defects and is suitable for large-scale screening.

However, the existing detection kit has a single structure, and only stores all the objects in a row, but is inconvenient to take and place in practical use and has the risk of mutual collision and damage in the transferring process.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the problem of providing an ELISA kit for detecting ciprofloxacin, which has simple structure, reasonable design and design in different areas; the kit body is provided with a detection area and a storage area, and an ELISA plate positioning assembly is arranged in the detection area, so that an ELISA plate can be horizontally placed and shaken during detection; set up in depositing the district and deposit drawer and second and deposit the drawer and deposit various reagents through first buffering deposit board and second buffering deposit the board, be convenient for get and put, can prevent to collide each other and lead to damaging simultaneously.

In order to solve the technical problem, the utility model discloses a technical scheme is: an ELISA kit for detecting ciprofloxacin comprises a box body and a box cover, wherein the box cover is hinged on the box body; the box body is divided into a detection area and a storage area from top to bottom, two groups of enzyme label plate positioning assemblies are arranged in the detection area, a partition plate is arranged in the middle of the storage area so as to divide the storage area into two parts, and a first storage drawer and a second storage drawer are respectively connected in a sliding manner;

a first buffer storage plate is clamped and fixed in the first storage drawer, and 6 bottles of ciprofloxacin standard solution and 2 bottles of ciprofloxacin antibody are clamped and fixed in the first buffer storage plate;

a second buffer storage plate is clamped and fixed in the second storage drawer, 1 bottle of enzyme-labeled secondary antibody, 1 bottle of stop solution, 2 bottles of substrate color development solution, 1 bottle of washing solution and 1 bottle of complex solution are clamped and fixed in the second buffer storage plate, and an enzyme-labeled plate and a specification are further clamped and fixed in the second buffer storage plate.

In the technical scheme, the detection area is used for detection, and when detection is carried out, the enzyme label plate can be placed in the positioning assembly for horizontal placement; arranging a first storage drawer and a second storage drawer for storing various solutions; the first buffer storage plate and the second buffer storage plate are arranged to clamp the solution bottles, so that the solution bottles can be conveniently stored and taken out, and the solutions in the bottles can be stored separately and can be effectively prevented from colliding with each other and being damaged.

Furthermore, a sliding channel is formed in the side wall of the bottom of the storage area, sliding connection blocks matched with the sliding channel are arranged on the side walls of the bottoms of the first storage drawer and the second storage drawer, and the sliding connection blocks are clamped in the sliding channel and are in sliding connection along the sliding channel.

Furthermore, a recovery groove, 8 positioning holes and 8 label bosses for recovering waste are arranged in the first buffer storage plate, the 8 positioning holes are respectively used for storing ciprofloxacin standard solution and ciprofloxacin antibody, and each label boss is respectively arranged at the side of each corresponding positioning hole so as to be convenient for pasting corresponding labels and distinguishing liquid in each positioning hole.

Further, a first sealing cover is further arranged in the first storage drawer, the first sealing cover is arranged on the upper side of the first buffering storage plate in a covering mode, a sealing plate is arranged at the bottom of the first sealing cover, and the sealing plate is inserted into the recycling groove to seal the recycling groove.

Furthermore, a clamping groove for clamping the ELISA plate, a storage groove for placing a specification, 6 fixing holes and 6 second label bosses are formed in the second buffer storage plate, and the 6 fixing holes are respectively used for fixedly storing a substrate developing solution, a stop solution, a washing solution, an ELISA secondary antibody and a complex solution; each second label boss is arranged at the side of the corresponding fixing hole respectively so as to be convenient for sticking the corresponding label and distinguishing the liquid in each fixing hole.

Furthermore, a second sealing cover is further arranged in the second storage drawer and covers the upper side of the second buffer storage plate and abuts against the second buffer storage plate.

Furthermore, a partition plate is fixedly arranged in the middle of the detection area so as to divide the detection area into two parts, a group of ELISA plate positioning assemblies is arranged in each part, each ELISA plate positioning assembly comprises two positioning plates and a plurality of buffer springs which are oppositely arranged, the bottom walls of the positioning plates are in sliding connection with the bottom wall of the detection area, and the two positioning plates are respectively fixed with the inner wall of the detection area and the side wall of the partition plate through the buffer springs;

a plurality of steps are arranged on the positioning plates in a step type design, and the steps of the two positioning plates are arranged oppositely, so that a corresponding fixing clamping groove is formed between the steps with different heights.

Further, a lifting handle is arranged on the top wall of the box cover, a protruding block is arranged on the bottom wall of the box cover, and the protruding block is matched with the opening of the detection area.

Further, be provided with two connecting plates on the lateral wall of lid, two correspond respectively on the connecting plate and be furnished with the second connecting plate, two the second connecting plate is fixed mounting respectively on the lateral wall that the drawer was deposited to first lateral wall and the second of depositing the drawer, set up between connecting plate and the second connecting plate and use latch fitting or fixed pin to carry out the connecting hole fixed so that fix both.

Furthermore, ciprofloxacin coupling antigen is coated in the hole of the ELISA plate; the concentrations of the ciprofloxacin standard solution in 6 bottles are respectively 0 mug/L, 2 mug/L, 6 mug/L, 18 mug/L, 48 mug/L and 144 mug/L; the enzyme-labeled secondary antibody is a ciprofloxacin anti-antibody labeled by horseradish peroxidase; the substrate color development liquid comprises a liquid A and a liquid B, wherein the liquid A is carbamide peroxide, and the liquid B is tetramethyl benzidine; the stop solution is 2mol/L sulfuric acid.

The utility model has the advantages and positive effects that:

the reagent kit of the utility model has simple structure, reasonable design and design in different areas; the kit body is provided with a detection area and a storage area, and an ELISA plate positioning assembly is arranged in the detection area, so that an ELISA plate can be horizontally placed and shaken during detection; set up in depositing the district and deposit drawer and second and deposit the drawer and deposit various reagents through first buffering deposit board and second buffering deposit the board, be convenient for get and put, can prevent to collide each other and lead to damaging simultaneously.

Drawings

FIG. 1 is a split view of an ELISA kit for detecting ciprofloxacin of the present invention;

FIG. 2 is a schematic diagram showing the overall structure of an ELISA kit for detecting ciprofloxacin according to the present invention;

FIG. 3 is a cross-sectional view of a box body of an ELISA kit for detecting ciprofloxacin according to the present invention;

FIG. 4 is a schematic structural diagram of a box cover in the ELISA kit for detecting ciprofloxacin of the present invention;

FIG. 5 is a schematic diagram of a first storage drawer of an ELISA kit for detecting ciprofloxacin of the present invention;

FIG. 6 is a schematic structural diagram of a first buffer storage plate in an ELISA kit for detecting ciprofloxacin of the present invention;

FIG. 7 is a schematic structural diagram of a first sealing cover in the ELISA kit for detecting ciprofloxacin of the present invention;

FIG. 8 is a schematic diagram of a second storage drawer of the ELISA kit for detecting ciprofloxacin of the present invention;

FIG. 9 is a schematic structural diagram of a second buffer storage plate in an ELISA kit for detecting ciprofloxacin according to the present invention;

FIG. 10 is a schematic structural diagram of a second sealing cover in the ELISA kit for detecting ciprofloxacin of the present invention;

in the figure: 1-box body, 2-box cover, 21-handle, 22-lug, 23-connecting plate, 24-second connecting plate, 25-connecting hole, 3-detection area, 31-partition board, 4-storage area, 41-sliding channel, 42-sliding joint block, 5-ELISA plate positioning component, 51-positioning plate, 511-step, 512-fixing slot, 52-buffer spring, 6-partition board, 7-first storage drawer, 8-second storage drawer, 9-first buffer storage plate, 91-recovery slot, 92-positioning hole, 93-label boss, 10-second buffer storage plate, 101-slot, 102-storage slot, 103-fixing hole, 104-second label boss, 11-first sealing cover, 111-sealing plate, 12-second sealing cover.

Detailed Description

The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings.

Example (b):

referring to fig. 1 and 2, an ELISA kit for detecting ciprofloxacin comprises a box body 1 and a box cover 2, wherein the box cover 2 is hinged on the box body 1; the box body 1 is divided into a detection area 3 and a storage area 4 from top to bottom, two groups of enzyme label plate positioning assemblies 5 are arranged in the detection area 3, a partition plate 6 is arranged in the middle of the storage area 4 so as to divide the storage area 4 into two parts, and a first storage drawer 7 and a second storage drawer 8 are respectively connected in a sliding manner; the detection area 3 is used for detection, and when detection is carried out, the enzyme label plate can be placed in the positioning assembly 5 for horizontal placement; a first storage drawer 7 and a second storage drawer 8 are provided for storing various solutions.

A first buffer storage plate 9 is fixedly clamped in the

first storage drawer

7, and 6 bottles of ciprofloxacin standard solution and 2 bottles of ciprofloxacin antibody are fixedly clamped in the first buffer storage plate 9;

a second buffer storage plate 10 is clamped and fixed in the

second storage drawer

8, 1 bottle of enzyme-labeled secondary antibody, 1 bottle of stop solution, 2 bottles of substrate color development solution, 1 bottle of washing solution and 1 bottle of complex solution are clamped and fixed in the second buffer storage plate 10, and an enzyme-labeled plate and a specification are further clamped and fixed in the second buffer storage plate 10. The first buffer storage plate 9 and the second buffer storage plate 10 are arranged to clamp the solution bottles, so that the solution bottles are convenient to store and take and place, and the solutions in the bottles are stored separately, so that the solutions can be effectively prevented from colliding with each other and being damaged.

Referring to fig. 1, 3 and 5, in the present embodiment, a sliding channel 41 is formed on a bottom side wall of the storage area 4, a sliding joint block 42 matched with the sliding channel 41 is arranged on a bottom side wall of the first storage drawer 7 and the second storage drawer 8, and the sliding joint block 42 is clamped in the sliding channel 41 and is slidably connected along the sliding channel 41. Wherein, set up sliding channel 41 and slide joint block 42 and match, be convenient for deposit drawer 7 with the second with the first inner wall of depositing drawer 8 and box body 1 spacing to be convenient for deposit drawer 7 with the second and deposit drawer 8 when pulling out and remain stable.

Referring to fig. 6, in the present embodiment, a

recycling groove

91, 8

positioning holes

92 and 8 label bosses 93 for recycling waste are formed in the first buffer storage plate 9, the 8 positioning holes 92 are respectively used for storing ciprofloxacin standard solution and ciprofloxacin antibody, and each label boss 93 is respectively arranged at the side of each corresponding positioning hole 92, so as to attach a corresponding label to distinguish the liquid in each positioning hole 92. A recovery tank 91 is arranged in the first buffer storage plate 9 and is used for containing used reagent bottles and enzyme label plates; the positioning hole 92 is used for storing and fixing the solution bottle, so that the position of the solution bottle is fixed, and the solution bottle is prevented from being poured and spilled; set up label boss 93 and be used for pasting and establish the label to distinguish different solution bottles, get when so that use and put, reduce the time of looking for.

Referring to fig. 5 and 7, a first sealing cover 11 is further provided in the first storage drawer 7, the first sealing cover 11 is covered on the upper side of the first buffer storage plate 9, a sealing plate 111 is provided on the bottom of the first sealing cover 11, and the sealing plate 111 is inserted into the recovery groove 91 to seal the recovery groove 91. The first sealing cover 11 is arranged to position the first buffer storage plate 9, wherein the sealing plate 111 seals the opening of the recovery groove 91, so that sundries in the recovery groove 91 can be prevented from contacting with nearby reagent bottles, and sanitation is guaranteed.

In this embodiment, referring to fig. 9, a slot 101 for clamping an elisa plate, a storage slot 102 for placing a specification, 6

fixing holes

103 and 6 second label bosses 104 are formed in the second buffer storage plate 10, and the 6 fixing holes 103 are respectively used for fixing and storing a substrate developing solution, a stop solution, a washing solution, an elisa secondary antibody and a complex solution; each second label boss 104 is respectively arranged at the side of the corresponding fixing hole 103 so as to be convenient for attaching the corresponding label to distinguish the liquid in each fixing hole 103. A clamping groove 101 is formed in the second buffer storage plate 10 and used for clamping an enzyme label plate, a storage groove 102 is used for storing a specification, and a fixing hole 103 is formed for clamping and fixing a solvent bottle, so that collision between the solvent bottles is prevented, and safe storage is guaranteed; set up second label boss 104 and be used for pasting and establish the label to distinguish different solution bottles, get when being convenient for use and put, reduce the time of looking for.

Referring to fig. 8 and 10, a second sealing cover 12 and a second sealing cover 12 are further disposed in the second storage drawer 8, and the second sealing cover 12 is disposed on the upper side of the second buffer storage plate 10 and abuts against the second buffer storage plate 10. The second sealing cover 12 is arranged on the upper side of the second buffer storage plate 10 and used for sealing the upper space of the second storage drawer 8, so that the normal and orderly storage of the articles stored inside is guaranteed, and the articles are prevented from leaking.

In the present embodiment, referring to fig. 3, a partition plate 31 is fixedly disposed in the middle of the detection area 3 so as to divide the detection area 3 into two parts, each part is provided with a set of elisa plate positioning assembly 5, each elisa plate positioning assembly 5 includes two positioning plates 51 and a plurality of buffer springs 52, which are oppositely disposed, the bottom walls of the positioning plates 51 are slidably connected with the bottom wall of the detection area 3, and the two positioning plates 51 are respectively fixed with the inner wall of the detection area 3 and the side wall of the partition plate 31 through the buffer springs 52; the ELISA plate positioning assembly 5 is used for clamping and preventing the ELISA plate so as to be convenient for use in detection; when the elisa plate clamp is used, the elisa plate is placed between the positioning plates 51 for clamping, and the two positioning plates 51 are separated from each other on the elisa plate, so that the buffer springs 52 generate corresponding elastic force to enable the two positioning plates 51 to fixedly clamp the elisa plate; when the slight oscillation is needed, the positioning plate 51 can be moved slightly, and the whole elisa plate can be driven to swing left and right under the driving of the buffer spring 52 to realize the oscillation.

The positioning plate 51 is provided with a plurality of steps 511 in a step-type design, and the steps 511 of the two positioning plates 51 are arranged oppositely, so that a corresponding fixing slot 512 is formed between the steps 511 with different heights. The positioning plate 51 is provided with a plurality of steps 511, so that a plurality of fixing clamping grooves 512 with different widths are formed between the two positioning plates 51, the positioning plate can be suitable for enzyme labeling plates with various widths, and the application range is wide.

In this embodiment, referring to fig. 4, a handle 21 is disposed on the top wall of the box cover 2, a protrusion 22 is disposed on the bottom wall of the box cover, and the protrusion 22 is adapted to the opening of the detection area 3. The handle 21 is arranged to facilitate the movement of the whole reagent kit.

In this embodiment, referring to fig. 2, two connecting plates 23 are disposed on the side wall of the box cover 2, the two connecting plates 23 are respectively and correspondingly matched with a second connecting plate 24, the two second connecting plates 24 are respectively and fixedly mounted on the side wall of the first storage drawer 7 and the side wall of the second storage drawer 8, and a connecting hole 25 for fixing the two connecting plates by using a locking piece or a fixing pin is disposed between the connecting plates 23 and the second connecting plates 24. Set up connecting plate 23 and second connecting plate 24 and be convenient for deposit drawer 8 with lid 2 and first deposit drawer 7 and second and fix to prevent that the three from opening, when fixing, can use lock or fixed pin cooperation nut with first connecting plate 23 and second connecting plate 24 lock die can, reopen when using can.

In the embodiment, ciprofloxacin coupling antigen is coated in the hole of the ELISA plate; the concentrations of the 6 bottles of ciprofloxacin standard solutions are respectively 0 mug/L, 2 mug/L, 6 mug/L, 18 mug/L, 48 mug/L and 144 mug/L; the enzyme-labeled secondary antibody is a ciprofloxacin anti-antibody labeled by horseradish peroxidase; the substrate color development liquid comprises a liquid A and a liquid B, wherein the liquid A is carbamide peroxide, and the liquid B is tetramethyl benzidine; the stop solution is 2mol/L sulfuric acid; the washing solution has a pH value of 7.2, and contains 0.01% of Tween-20, 3g/L of sodium azide preservative and 0.01mol/L of phosphate buffer solution, wherein the percentage is weight volume percentage; the complex solution is phosphate buffer solution with pH value of 7.0 and 0.01 mol/L. Wherein, the ciprofloxacin coating antigen and the ciprofloxacin monoclonal antibody in the embodiment are both from Wuhanyangda biotechnology limited company; the enzyme-labeled secondary antibody is obtained by coupling ciprofloxacin anti-antibody with horseradish peroxidase (HRP), wherein the ciprofloxacin anti-antibody is obtained by immunizing a goat serving as an immune animal and a goat without a pathogen by using a ciprofloxacin monoclonal antibody as an immunogen.

The utility model discloses a detect ELISA kit of ciprofloxacin when preparing:

1. preparing an enzyme label plate: diluting the coating source to 15 μ g/mL with a coating buffer solution (pH9.6 carbonate), adding 100 μ l to each well, incubating at 4 ℃ in the dark for 16h, pouring off the liquid in the wells, washing with a washing solution for 1 time, standing for 30s, patting to dry, adding 200 μ l of a blocking solution to each well, incubating at 37 ℃ in the dark for 2h, pouring off the liquid in the wells, patting to dry, and performing vacuum sealing and preservation with an aluminum film after drying.

2. Assembling a kit: firstly, a first buffer storage plate 9 and a second buffer storage plate 10 are respectively placed in a first storage drawer 7 and a second storage drawer 8; secondly, respectively placing 6 bottles of ciprofloxacin standard solution and ciprofloxacin antibody in positioning holes 92 of a first buffer storage plate 9, and attaching corresponding labels to label bosses 93 corresponding to the positioning holes 92; thirdly, clamping the ELISA plate in a clamping groove 101 of the second buffer storage plate 10, placing the specification in a storage groove 102, respectively clamping the substrate developing solution, the stop solution, the washing solution, the ELISA secondary antibody and the redissolution in fixed holes 103, and attaching corresponding labels to second label bosses 104 corresponding to the fixed holes 103; fourthly, respectively covering the first sealing cover 11 and the second sealing cover 12 in the first storage drawer 7 and the second storage drawer 8, and combining the first storage drawer 7 and the second storage drawer 8 with the box body 1; the box cover 2 is buckled, and the lock penetrates through the two connecting holes 25 to lock the connecting plate 23 and the second connecting plate 24, namely the box cover 2 and the box body 1 are fixed, and the first storage drawer 7, the second storage drawer 8 and the box body 1 are fixed and do not move any more.

The ELISA kit for detecting ciprofloxacin of the utility model has the following detection steps:

1. placing the enzyme label plate into a proper fixed clamping groove 512 and keeping the enzyme label plate horizontal; adding 50 mul of standard solution/sample into the corresponding micropores of the ELISA plate, adding 50 mul/pore of the mixed solution of the antibody and the enzyme-labeled secondary antibody (10:1), lightly shaking and uniformly mixing, covering the mixture with a cover plate film, and reacting for 30min in a dark environment at 25 ℃.

2. Carefully uncovering the cover plate film, drying liquid in the holes, fully washing the holes for 4-5 times by using 250 mu l of washing liquid at intervals of 10s, and patting the holes dry by using absorbent paper (bubbles which are not removed after patting the holes dry can be slightly punctured by using an unused gun head).

3. Adding 50 μ l/hole of the substrate solution A, adding 50 μ l/hole of the substrate solution B, gently shaking, mixing, covering with a cover plate, and developing at 25 deg.C in dark environment for 15 min.

4. Adding 50 mul/hole of stop solution, lightly shaking and mixing, setting the detection wavelength of an enzyme-labeling instrument to be 450nm and the reference wavelength to be 620nm, and measuring the OD value of each hole.

The above detailed description of the embodiments of the present invention is only for the preferred embodiments of the present invention, and the present invention should not be considered as limiting the scope of the present invention. All the equivalent changes and improvements made according to the application scope of the present invention should still fall within the patent coverage of the present invention.

Claims

1. An ELISA kit for detecting ciprofloxacin, which is characterized in that: the box comprises a box body (1) and a box cover (2), wherein the box cover (2) is hinged on the box body (1); the box body (1) is divided into a detection area (3) and a storage area (4) from top to bottom, two groups of enzyme label plate positioning assemblies (5) are arranged in the detection area (3), a partition plate (6) is arranged in the middle of the storage area (4) so as to divide the storage area (4) into two parts, and a first storage drawer (7) and a second storage drawer (8) are respectively connected in a sliding manner;

a first buffer storage plate (9) is fixedly clamped in the first storage drawer (7), and 6 bottles of ciprofloxacin standard solution and 2 bottles of ciprofloxacin antibody are fixedly clamped in the first buffer storage plate (9);

a second buffer storage plate (10) is clamped and fixed in the second storage drawer (8), 1 bottle of enzyme-labeled secondary antibody, 1 bottle of stop solution, 2 bottles of substrate color development solution, 1 bottle of washing solution and 1 bottle of complex solution are clamped and fixed in the second buffer storage plate (10), and an enzyme label plate and a specification are further clamped and arranged in the second buffer storage plate (10).

2. The ELISA kit for detecting ciprofloxacin according to claim 1, wherein: the utility model discloses a drawer storage device, including storage area (4), storage area (8), sliding channel (41) have been seted up on the bottom lateral wall of storage area (4), first storage drawer (7) and second are deposited and are provided with on the bottom lateral wall of drawer (8) with sliding channel (41) assorted sliding connection piece (42), sliding connection piece (42) card is established in sliding channel (41) and along sliding channel (41) sliding connection.

3. The ELISA kit for detecting ciprofloxacin according to claim 1, wherein: a recovery groove (91), 8 positioning holes (92) and 8 label bosses (93) for recovering waste are arranged in the first buffer storage plate (9), 8 positioning holes (92) are respectively used for storing ciprofloxacin standard solution and ciprofloxacin antibody, and each label boss (93) is respectively arranged at the side of each corresponding positioning hole (92) so as to be convenient for pasting corresponding labels and distinguish liquid in each positioning hole (92).

4. The ELISA kit for detecting ciprofloxacin according to claim 3, wherein: still be provided with first sealed lid (11) in first storage drawer (7), the upside of first buffering storage plate (9) is located in first sealed lid (11) lid, the bottom of first sealed lid (11) is provided with closing plate (111), closing plate (111) are inserted and are established in accumulator (91) and seal accumulator (91).

5. The ELISA kit for detecting ciprofloxacin according to claim 1, wherein: a clamping groove (101) for clamping an enzyme label plate, a storage groove (102) for placing a specification, 6 fixing holes (103) and 6 second label bosses (104) are formed in the second buffer storage plate (10), and the 6 fixing holes (103) are respectively used for fixedly storing a substrate color development solution, a stop solution, a washing solution, an enzyme-labeled secondary antibody and a complex solution; each second label boss (104) is arranged at the side of the corresponding fixing hole (103) so as to be convenient for attaching the corresponding label to distinguish the liquid in each fixing hole (103).

6. The ELISA kit for detecting ciprofloxacin according to claim 5, wherein: a second sealing cover (12) is further arranged in the second storage drawer (8), the second sealing cover (12) is covered on the upper side of the second buffer storage plate (10) and is propped against the second buffer storage plate (10) through the second sealing cover (12).

7. The ELISA kit for detecting ciprofloxacin according to claim 1, wherein: the detection area (3) is divided into two parts by a partition plate (31) fixedly arranged in the middle of the detection area (3), a group of ELISA plate positioning assemblies (5) is arranged in each part, each ELISA plate positioning assembly (5) comprises two positioning plates (51) and a plurality of buffer springs (52) which are oppositely arranged, the bottom walls of the positioning plates (51) are in sliding connection with the bottom wall of the detection area (3), and the two positioning plates (51) are respectively fixed with the inner wall of the detection area (3) and the side wall of the partition plate (31) through the buffer springs (52);

a plurality of steps (511) are arranged on the positioning plates (51) in a step type design, the steps (511) of the two positioning plates (51) are arranged oppositely, and therefore a corresponding fixed clamping groove (512) is formed between the steps (511) with different heights.

8. The ELISA kit for detecting ciprofloxacin according to claim 1, wherein: the portable detection box is characterized in that a handle (21) is arranged on the top wall of the box cover (2), a convex block (22) is arranged on the bottom wall of the box cover, and the convex block (22) is matched with an opening of the detection area (3).

9. The ELISA kit for detecting ciprofloxacin according to claim 1, wherein: be provided with two connecting plates (23) on the lateral wall of lid (2), two correspond respectively on connecting plate (23) and be furnished with second connecting plate (24), two second connecting plate (24) fixed mounting respectively is on the lateral wall that drawer (7) were deposited to the first lateral wall of depositing and the lateral wall that drawer (8) were deposited to the second, set up between connecting plate (23) and second connecting plate (24) and use latch fitting or fixed pin to carry out fixed connecting hole (25) so that fix both.

10. The ELISA kit for detecting ciprofloxacin according to claim 1, wherein: ciprofloxacin coupling antigen is coated in the holes of the ELISA plate; the concentrations of the ciprofloxacin standard solution in 6 bottles are respectively 0 mug/L, 2 mug/L, 6 mug/L, 18 mug/L, 48 mug/L and 144 mug/L; the enzyme-labeled secondary antibody is a ciprofloxacin anti-antibody labeled by horseradish peroxidase; the substrate color development liquid comprises a liquid A and a liquid B, wherein the liquid A is carbamide peroxide, and the liquid B is tetramethyl benzidine; the stop solution is 2mol/L sulfuric acid.