CN211947060U - Photosynthetic bacteria reactor - Google Patents
Photosynthetic bacteria reactor Download PDFInfo
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- CN211947060U CN211947060U CN202020498675.4U CN202020498675U CN211947060U CN 211947060 U CN211947060 U CN 211947060U CN 202020498675 U CN202020498675 U CN 202020498675U CN 211947060 U CN211947060 U CN 211947060U
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- 241000894006 Bacteria Species 0.000 title claims abstract description 120
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 114
- 239000007788 liquid Substances 0.000 claims abstract description 65
- 238000010438 heat treatment Methods 0.000 claims abstract description 48
- 238000012258 culturing Methods 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims description 77
- 239000011521 glass Substances 0.000 claims description 30
- 238000003756 stirring Methods 0.000 claims description 26
- 230000001954 sterilising effect Effects 0.000 claims description 20
- 238000007789 sealing Methods 0.000 claims description 14
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 37
- 238000007872 degassing Methods 0.000 description 13
- 238000000034 method Methods 0.000 description 13
- 230000008569 process Effects 0.000 description 8
- 230000006378 damage Effects 0.000 description 7
- 230000000249 desinfective effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000012423 maintenance Methods 0.000 description 5
- 238000003825 pressing Methods 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 238000010907 mechanical stirring Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 239000013529 heat transfer fluid Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
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Abstract
The application provides a photosynthetic bacteria reactor, belongs to photosynthetic bacteria's cultivation technical field. The photosynthetic bacteria reactor comprises a reaction device, the reaction device comprises an inner cylinder and an outer cylinder, the inner cylinder is arranged in the outer cylinder, the inner cylinder is provided with a first groove for culturing photosynthetic bacteria, and an annular groove for containing heat-conducting liquid is arranged between the inner cylinder and the outer cylinder. This reactor can carry out the liquid bath heating through the outer heat conduction liquid of inner jar to the inner jar, avoids the solution direct contact of heating device and inner jar and pollutes solution to avoid photosynthetic bacteria's cultivation to suffer the pollution, and the heating is more even, is favorable to photosynthetic bacteria's growth.
Description
Technical Field
The application relates to the technical field of culture of photosynthetic bacteria, in particular to a photosynthetic bacteria reactor.
Background
The photosynthetic bacteria can be widely applied to the aspects of agriculture, animal husbandry, environmental protection and the like as probiotics, along with the continuous deepening of the research and the application of the photosynthetic bacteria, how to realize the expanded culture of the photosynthetic bacteria is the first problem facing at present, and the premise of realizing the large-scale application of the photosynthetic bacteria is to solve the problem of the expanded culture of the photosynthetic bacteria.
In the prior art, the photosynthetic bacteria are generally polluted in the process of culturing the photosynthetic bacteria.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a photosynthetic bacteria reactor can improve the contaminated problem of photosynthetic bacteria culture's in-process.
The embodiment of the application provides a photosynthetic bacteria reactor, including reaction unit, reaction unit includes inner casing and outer jar. The inner jar is arranged in the outer jar, the inner jar is provided with a first groove for culturing photosynthetic bacteria, and an annular groove for containing heat-conducting liquid is arranged between the inner jar and the outer jar.
When the inner jar needs to be used for culturing photosynthetic bacteria, the heat conducting liquid is directly added into the outer jar to heat the inner jar in a liquid bath manner, so that the situation that the photosynthetic bacteria are polluted due to the contact of the heater and the solution (nutrient solution and bacteria liquid) in the inner jar can be avoided, the heating is more uniform, and the solution in the inner jar is in a proper temperature range, thereby being beneficial to the growth of the photosynthetic bacteria.
In an alternative embodiment, the inner cylinder and the outer cylinder are both transparent cylinders, and the cylinder wall of the outer cylinder is further provided with a light-emitting device.
The inner and outer jars are made of transparent materials, the growth condition of the photosynthetic bacteria can be known by observing the color of the solution in the inner jar, and the light and the sunlight generated by the light-emitting device can penetrate through the transparent jar, so that the illumination requirement of the photosynthetic bacteria can be met.
In an alternative embodiment, the cylinder wall of the outer cylinder is provided with a heating device configured to heat the heat conducting liquid in the annular groove.
Heating device sets up at the outer jar, can effectively heat the heat conduction liquid to the temperature of control heat conduction liquid, thereby carry out the even heating to the inner jar.
In an alternative embodiment, the inner casing and the outer casing are both glass casings, the heating device is fixed on the inner wall of the outer casing through a sucker, and the light-emitting device is fixed on the outer wall of the outer casing through a sucker.
The glass jar has good light transmission effect and good heat conduction effect, the light-emitting device is arranged on the outer wall of the outer jar, and light generated by the light-emitting device can well penetrate through the jar wall of the outer jar and the jar wall of the inner jar so as to promote the culture of photosynthetic bacteria; and the heat in the heat-conducting liquid in the annular groove formed by the outer cylinder and the inner cylinder can be better transferred into the inner cylinder, and the temperature of the heat-conducting liquid is basically consistent with the reaction temperature in the inner cylinder, so that the control of the reaction temperature of the inner cylinder is more accurate and stable.
In optional embodiment, still include the inlet means, have the feed liquor chamber in the inlet means, feed liquor chamber and first recess intercommunication are provided with the first degassing unit that is used for disinfecting in the feed liquor intracavity, are provided with the second degassing unit that is used for disinfecting in the first recess.
When the reactor is started, the second disinfection device is started to comprehensively disinfect the inner cylinder, then the nutrient solution is disinfected, and the nutrient solution is circulated in the liquid inlet cavity and is disinfected and disinfected by the first disinfection device; then get into in the first recess by the disinfection of second degassing unit sterilization, can carry out secondary sterilization to the nutrient solution, disinfect more thoroughly to avoid other miscellaneous fungus to bring about the influence to photosynthetic bacteria growth, avoid photosynthetic bacteria to receive the pollution. And the nutrient solution after primary sterilization directly enters the first groove without external transfer, thereby avoiding secondary pollution of the nutrient solution.
In optional embodiment, be provided with the sealed lid of detachable on the first recess, inlet means includes feed liquor pipe and disinfection pipe, and the pipeline of disinfection pipe and the pipeline of feed liquor pipe form the feed liquor chamber, and the one end and the sealed lid of feed liquor pipe are connected, and with first recess intercommunication, the other end and the disinfection pipe intercommunication of feed liquor pipe, and the one end of keeping away from the feed liquor pipe of disinfection pipe is provided with the inlet, sets up first degassing unit in the disinfection pipe.
Through the effect of sealed lid, can form anaerobic environment in the inner tank to the cultivation of photosynthetic bacteria is favorable to. And before the nutrient solution enters the first groove, the nutrient solution is firstly sterilized in the disinfection pipe for the first time, then flows through the liquid inlet pipe, and is then sterilized in the first groove for the second time, so that the sterilization effect is better.
In an optional embodiment, the disinfection tube is positioned above the sealing cover, the disinfection tube is horizontally arranged, and the liquid inlet tube is vertically arranged.
When the nutrient solution flows in the horizontally disinfecting pipe, the flowing speed is relatively slow, and the nutrient solution can flow into the first groove quickly in the disinfecting pipe for a long time so as to sterilize thoroughly for the first time and then enter the first groove through the vertically arranged liquid inlet pipe.
In an alternative embodiment, the magnetic stirrer is further included, and the bottom plate of the inner cylinder is a glass plate. The magnetic stirrer is arranged below the glass plate and is configured to form a magnetic field in the first groove so as to control the stirrer in the first groove to rotate.
Magnetic stirrers can make the stirring son in the glass container rotate, compares with traditional puddler agitator, if when traditional agitator broke down the maintenance, need tear the puddler open, then carries out the maintenance of instrument, can destroy the anaerobic environment in the container, and can make photosynthetic bacteria's cultivation suffer the pollution. And use magnetic stirrers to stir, set up the stirring in the inner tank, do not have mechanical connection's relation between magnetic stirrers's main part and the inner tank, can make things convenient for the change and the maintenance of magnetic stirrers main part, avoid photosynthetic bacteria culture's in-process to suffer the pollution.
In an alternative embodiment, the glass panel extends in the direction of the outer cylinder and is sealingly connected to the lower end of the outer cylinder, the outer wall of the inner cylinder, the glass panel and the inner wall of the outer cylinder forming an annular groove.
The bottom wall of the first groove and the bottom wall of the annular groove share the same glass plate, so that the thickness of the glass plate in the reactor can be reduced, the distance between the main body of the magnetic stirrer and the stirrer is reduced, the stirring motion of the stirrer is facilitated, and the stirring effect is better.
In an alternative embodiment, the magnetic stirrer further comprises a control device, and the control device is electrically connected with the magnetic stirrer, the heating device and the light-emitting device.
The operation of the stirring, heating and light-emitting devices can be controlled by the control device, so that the automatic culture of the photosynthetic bacteria can be realized, and the large-scale production can be realized.
In an optional embodiment, the magnetic stirrer further comprises a control panel, wherein a power control key, a heating control key, a light-emitting control key and a stirring control key are arranged on the control panel, the power control key is electrically connected with the control device to control the control device to be powered on or powered off, the heating control key is electrically connected with the control device to control the heating device to be turned on or off, the light-emitting control key is electrically connected with the control device to control the light-emitting device to be turned on or off, and the stirring control key is electrically connected with the control device to control the magnetic stirrer to be turned on or off.
The control keys are respectively arranged to independently and accurately control each condition, so that the control is more accurate, and the adjustment of the conditions required by the growth of the photosynthetic bacteria is more facilitated.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments are briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present application and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained from the drawings without inventive efforts and also belong to the protection scope of the present application.
Fig. 1 is a schematic view of a first structure of a photosynthetic bacteria reactor provided in an embodiment of the present application;
FIG. 2 is a cross-sectional view A-A of FIG. 1;
fig. 3 is a schematic view of a second structure of a photosynthetic bacteria reactor provided in the embodiment of the present application.
Icon: 10-inner cylinder; 11-a first groove; 12-a sealing cover; 13-an exhaust valve; 14-a glass plate; 15-a first drain pipe; 20-outer cylinder; 21-an annular groove; 22-a second drain; 30-a heating device; 40-a light emitting device; 50-a liquid inlet device; 51-a liquid inlet pipe; 52-sterilizing the tube; 53-liquid inlet; 54-a support bar; 60-a second disinfection device; 61-a first disinfection device; 70-a magnetic stirrer; 80-a support frame; 90-a box body; 92-a distribution box arrangement; 93-control panel.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described below with reference to the drawings in the embodiments of the present application.
The inventors have found that there is often a problem of contamination of photosynthetic bacteria when they are cultured on a large scale. The inventors have further studied and found that the cause of the contamination of the photosynthetic bacteria is mainly as follows:
(1) in the prior art, the culture of photosynthetic bacteria needs to be carried out at a proper temperature range (for example, 30-36 ℃), which is usually higher than the indoor environment temperature, so that the solution (nutrient solution + bacteria solution) in the container needs to be heated when the photosynthetic bacteria are cultured. Generally, a heater is disposed on the inner wall of the container, and the solution in the container is directly heated by the heater, so that the photosynthetic bacteria are cultured in a proper temperature range.
(2) In the prior art, before the nutrient solution enters the container to culture the photosynthetic bacteria, the nutrient solution is generally required to be sterilized and disinfected, so that the photosynthetic bacteria are prevented from being polluted by the mixed bacteria in the nutrient solution. However, when the nutrient solution is transferred to a container after the nutrient solution is sterilized, the nutrient solution is secondarily contaminated, so that the photosynthetic bacteria are contaminated during the cultivation process. Meanwhile, the nutrient solution is not thoroughly sterilized at one time, and photosynthetic bacteria may be polluted.
(3) After inoculating the photosynthetic bacteria solution into the nutrient solution, it is usually necessary to perform a culture with stirring by providing a stirring device in order to perform a large-scale culture of the photosynthetic bacteria. In the prior art, a container is usually made of a metal material, when photosynthetic bacteria are cultured, a mechanical stirring device is usually used for stirring a solution in the container, a stirring motor is arranged outside the container, and a stirring rod penetrates through the wall of the container and then enters the container for stirring; on the other hand, when the agitating unit needed the maintenance if taking place to damage, then need dismantle the puddler, carry out agitator motor's maintenance, when dismantling the puddler, can destroy the anaerobic environment in the container, can make photosynthetic bacteria's cultivation environment suffer the pollution.
In order to solve the problem of pollution in the process of culturing photosynthetic bacteria, the inventor provides a photosynthetic bacteria reactor, which has the following specific structure:
fig. 1 is a schematic view of a first structure of a photosynthetic bacteria reactor provided in this embodiment; FIG. 2 is a cross-sectional view A-A of FIG. 1; fig. 3 is a schematic view of a third structure of the photosynthetic bacteria reactor provided in this embodiment. Referring to fig. 1-3, in the present embodiment, the reaction device of the photosynthetic bacteria reactor comprises an inner casing 10 and an outer casing 20, the inner casing 10 is disposed in the outer casing 20, the inner casing 10 has a first groove 11 for culturing photosynthetic bacteria, and an annular groove 21 for containing heat transfer fluid is formed between the inner casing 10 and the outer casing 20.
Alternatively, the heat conducting liquid may be water, oil, or other liquid capable of conducting heat, and the application is not limited thereto, and the heat conducting liquid is water as an example for explanation. The specific heat capacity of water is higher, and the light transmissivity is better, and when cultivateing photosynthetic bacteria, photosynthetic bacteria (fungus liquid) and nutrient solution have been put into to first recess 11, and the annular groove 21 is built-in to put water. The water in the annular groove 21 is heated firstly, and then the solution in the inner cylinder 10 is heated in a water bath mode, so that the solution can be heated more uniformly, and the growth of photosynthetic bacteria is facilitated. And the heater is not required to be contacted with the solution in the first groove 11, so that the photosynthetic bacteria are prevented from being polluted in the culture process. Because the light transmissivity of water is better, external light can pass through water and shine to the inner jar 10 in to cultivate the photosynthetic bacteria, both can satisfy the heating demand of the solution in first recess 11, can satisfy the illumination demand of the solution in first recess 11 again.
In order to realize water bath heating, the water is heated by the heating device 30 to make the water in the annular groove 21 reach a proper temperature range, and then the solution in the first groove 11 is heated by the water bath mode to make the photosynthetic bacteria culture in the proper temperature range.
In order to heat the water with high efficiency by the heating device 30, optionally, the heating device 30 is arranged on the cylinder wall of the outer cylinder 20, and the heating device 30 is configured to heat the heat-conducting liquid in the annular groove 21, that is, to heat the water in the annular groove 21. Further, by providing the heating means 30 on the inner wall of the outer tub 20, the water can be directly contacted with the heating means 30 to efficiently heat the water.
Alternatively, the inner casing 10 and the outer casing 20 are both square structures, and the heating device 30 may be bar-shaped heating pipes (there may be two or four heating pipes), which are vertically installed on two opposite sides of the inner wall of the outer casing 20, respectively, or one heating pipe is vertically installed on each of four inner walls of the outer casing 20, so as to rapidly and uniformly heat water. In other embodiments, a strip-shaped heating pipe may also be horizontally installed on the inner wall of the outer cylinder 20, and the embodiment of the present application is not limited.
In order to further prevent the photosynthetic bacteria from being polluted in the culture process, the inner jar 10 and the outer jar 20 are transparent jars, the jar wall of the outer jar 20 is provided with the light emitting device 40, and light generated by the light emitting device 40 and external sunlight can penetrate through the jar wall of the outer jar 20 and the jar wall of the inner jar 10 and water in the annular groove 21, so that the photosynthetic bacteria can be illuminated to grow. And the solution does not contact with the light emitting device 40, thereby preventing the culture of the photosynthetic bacteria from being polluted.
Further, if the inner casing 10 and the outer casing 20 are both of a square structure, the light emitting device 40 may be a strip-shaped light emitting lamp (12, 16 or 8 strips may be provided), the strip-shaped light emitting lamps are horizontally installed on four outer walls of the outer casing 20, respectively, and three, four or two light emitting lamps are horizontally installed on each outer wall of the outer casing 20, respectively, so as to satisfy the illumination condition required for the growth of the photosynthetic bacteria. In other embodiments, a strip-shaped light emitting tube may also be vertically installed on the outer wall of the outer casing 20, and the embodiment of the present application is not limited thereto.
In other embodiments, the inner cylinder 10 and the outer cylinder 20 may have a column structure or other irregular shapes, which is not limited in this application. Accordingly, the shapes of the light emitting device 40 and the heating device 30 may be adjusted accordingly, or the installation manner thereof may be adjusted, etc., so that the light emitting device 40 and the heating device 30 can be installed on the cylinder wall of the outer cylinder 20.
In the embodiment of the present application, the transparent cylinder may be made of a glass material or other transparent organic polymer materials. If the transparent casing is a glass casing, the heating device 30 is fixed to the inner wall of the outer casing 20 by a suction cup, and the light emitting device 40 is fixed to the outer wall of the outer casing 20 by a suction cup. The heating pipe and the luminous lamp tube are fixed through the sucker, so that the damage to the glass jar can be avoided, and the heating pipe and the luminous lamp tube are convenient to install and detach. Simultaneously, the light transmissivity and the heat conductivity of glass jar are all better, and when the water bath heating, the temperature of water is unanimous basically with the temperature to the solution in the first recess 11, makes photosynthetic bacteria's reaction temperature's control more accurate, stable, and the light-emitting lamp sends ground light and can be utilized by photosynthetic bacteria high-efficiently, do benefit to photosynthetic bacteria's growth.
In order to put the nutrient solution into the first groove 11, the photosynthetic bacteria bacterial liquid is inoculated on the nutrient solution in the first groove 11. The photosynthetic bacteria reactor further comprises a liquid inlet device 50, a liquid inlet cavity (not shown) is arranged in the liquid inlet device 50, the liquid inlet cavity is communicated with the first groove 11, a first disinfection device 61 for sterilization is arranged in the liquid inlet cavity, and a second disinfection device 60 for sterilization is arranged in the first groove 11.
When starting the reactor, open second degassing unit 60 earlier and disinfect comprehensively inner cylinder 10, then disinfect to the nutrient solution, the nutrient solution passes through inlet means 50 feed liquor in first recess 11, can disinfect to the nutrient solution through the first degassing unit 61 in the feed liquor intracavity earlier, then directly shift the nutrient solution to first recess 11 through the feed liquor chamber in, do not need the step of extra transfer, avoid transfer process in the nutrient solution to suffer secondary pollution. And the nutrient solution enters the first groove 11 and is sterilized again through the second sterilizing device 60, and after twice sterilization, the sterilization is more thorough, and the photosynthetic bacteria are further prevented from being polluted in the culture process.
Optionally, a detachable sealing cover 12 is disposed on the first groove 11, and when the photosynthetic bacteria are cultured, the sealing cover 12 is used to seal the first groove 11, so that an anaerobic environment is formed in the first groove 11, so as to facilitate the growth of the photosynthetic bacteria.
Further, the liquid inlet device 50 comprises a liquid inlet pipe 51 and a disinfection pipe 52, a liquid inlet cavity is formed by a pipeline of the disinfection pipe 52 and a pipeline of the liquid inlet pipe 51, one end of the liquid inlet pipe 51 is connected with the sealing cover 12 and communicated with the first groove 11, the other end of the liquid inlet pipe 51 is communicated with the disinfection pipe 52, a liquid inlet 53 is arranged at one end, far away from the liquid inlet pipe 51, of the disinfection pipe 52, and a first disinfection device 61 is arranged in the disinfection pipe 52.
Before entering the first groove 11, the nutrient solution is sterilized in the sterilizing tube 52 for the first time, then flows through the liquid inlet tube 51, and then is sterilized in the first groove 11 for the second time, so that the sterilizing effect is better. To avoid contamination of the culture of photosynthetic bacteria.
Alternatively, the sterilizing duct 52 is positioned above the sealing cover 12, the sterilizing duct 52 is horizontally disposed, and the liquid feeding duct 51 is vertically disposed. Nutrient solution gets into disinfection pipe 52 from inlet 53 earlier in, because disinfection pipe 52 level sets up, the speed that the nutrient solution flows is slower relatively, can be for a long time in disinfection pipe 52 through a degassing unit 61 disinfection of disinfecting to carry out thorough first time sterilization, then in the inlet pipe 51 entering first recess 11 through vertical setting, can flow into first recess 11 through the action of gravity with the nutrient solution in the disinfection pipe 52.
Further, in order to install the disinfection tube 52 above the sealing cover 12, two support rods 54 are respectively arranged at two ends of the disinfection tube 52, the support rods 54 are vertically arranged, and one end of each support rod 54, which is far away from the disinfection tube 52, is connected to the sealing cover 12, so as to horizontally install the disinfection tube 52.
Optionally, a first valve is disposed on the liquid inlet pipe 51, and the liquid inlet pipe 51 can be controlled to be opened or closed by the valve. Optionally, the first valve may be a ball valve, a butterfly valve, a stop valve, etc., and the present application is not limited thereto, as long as the valve capable of intercepting flow can be the first valve.
Further, the upper end pipe wall of the liquid inlet pipe 51 is connected with the disinfection pipe 52, the liquid inlet pipe 51 is communicated with the disinfection pipe 52, the lower end pipe wall of the liquid inlet pipe 51 is connected with the sealing cover 12, and the liquid inlet pipe 51 is communicated with the first groove 11. In order to adjust the pressure in the first groove 11, an exhaust valve 13 is provided on the sealing cover 12 to exhaust the internal pressure of the first groove 11 during the cultivation of the photosynthetic bacteria.
In the embodiment of the present application, the first sterilizing device 61 and the second sterilizing device 60 may be both ultraviolet sterilizers. In other embodiments, the first disinfection device 61 and the second disinfection device 60 may also be radiation sterilizers or filter sterilizers, etc., and only disinfection devices capable of sterilizing bacteria in the nutrient solution are within the scope of the present application.
In order to stir the nutrient solution and the photosynthetic bacteria in the first groove 11 so that the photosynthetic bacteria can be cultured on a large scale. The photosynthetic bacteria reactor also comprises a magnetic stirrer 70, and the bottom plate of the inner cylinder 10 is a glass plate 14; a magnetic stirrer 70 is disposed below the glass sheet 14, the magnetic stirrer 70 being configured to form a magnetic field within the first recess 11 to control the rotation of the stirrer within the first recess 11.
The use of the glass sheet 14 does not damage the magnetic field generated by the magnetic stirrer 70. Stir solution through magnetic stirrers 70, even the agitator takes place to damage, direct change magnetic stirrers 70 the main part can, can make and realize stable anaerobic environment in the first recess 11, avoid photosynthetic bacteria's cultivation environment to suffer destruction, avoid photosynthetic bacteria to suffer pollution at the in-process of cultivateing.
Optionally, the glass panel 14 extends in a direction towards the outer cylinder 20 and is sealingly connected to the lower end of the outer cylinder 20, the outer wall of the inner cylinder 10, the glass panel 14 and the inner wall of the outer cylinder 20 forming an annular groove 21. Optionally, the lower ends of the outer cylinders 20 are each sealingly connected to the glass sheets 14 by a sealant.
The bottom wall of the first groove 11 and the bottom wall of the annular groove 21 share the same glass plate 14, the thickness of the bottom plate of the first groove 11 can be made small, and the distance between the main body of the magnetic stirrer 70 and the stirrer can be made relatively small for the stirring motion of the stirrer.
Optionally, a support frame 80 is further provided at the lower end of the glass plate 14, and the reaction device is supported by the support frame 80. The support bracket 80 is further provided with a box 90, and the main body of the magnetic stirrer 70 is arranged in the box 90, so that the main body of the magnetic stirrer 70 is basically contacted with the glass plate 14 but the glass plate 14 does not cause pressure to the main body of the magnetic stirrer 70.
After the culture of the photosynthetic bacteria is completed, the mixture of the nutrient solution and the photosynthetic bacteria in the first groove 11 needs to be discharged, and the water in the annular groove 21 needs to be discharged. Optionally, a first drain is provided on the glass plate 14, the first drain is located on the bottom wall of the first groove 11, a first drain pipe 15 is connected to the first drain, and the solution in the first groove 11 is discharged through the first drain pipe 15. Further, a second valve is provided on the first drain pipe 15 to control the conduction or the cutoff of the first drain pipe 15.
The glass plate 14 is further provided with a second water outlet which is positioned on the bottom wall of the annular groove 21, a second water discharge pipe 22 is connected to the second water outlet, and water in the annular groove 21 is discharged through the second water discharge pipe 22 through the second water outlet. Further, a third valve is provided on the second drain pipe 22 to control the conduction or the cutoff of the second drain pipe 22.
Alternatively, the second drain port may also be provided at the lower end of the cylinder wall of the outer cylinder 20, and may also drain the water in the annular groove 21.
In order to more conveniently realize automatic culture of the photosynthetic bacteria, the method is convenient for large-scale production. The photosynthetic bacteria reactor further includes a control device (not shown) electrically connected to the magnetic stirrer 70, the heating device 30 and the light emitting device 40.
The control unit is electrically connected to the electric box assembly 92 and is connected to the ac power source in the room through an input terminal of the electric box assembly 92. A power distribution box assembly 92 may be disposed below the box 90.
Optionally, the photosynthetic bacteria reactor further comprises a control panel 93, a power control key, a heating control key, a light-emitting control key and a stirring control key are arranged on the control panel 93, the power control key is electrically connected with the control device to control the control device to be powered on or powered off, the heating control key is electrically connected with the control device to control the heating device 30 to be turned on or off, the light-emitting control key is electrically connected with the control device to control the light-emitting device 40 to be turned on or off, and the stirring control key is electrically connected with the control device to control the magnetic stirrer 70 to be turned on or off.
Further, the control panel 93 is further provided with a first disinfection control key and a second disinfection control key, the first disinfection control key is electrically connected with the control device to control the first disinfection device 61 to be opened or closed, and the second disinfection control key is electrically connected with the control device to control the second disinfection device 60 to be opened or closed. The control keys are respectively arranged to independently and accurately control each condition, so that the control is more accurate, and the adjustment of the conditions required by the growth of the photosynthetic bacteria is more facilitated.
Optionally, the control panel 93 may be a touch screen or a key panel, and the control key may be a button or a touch key on the touch screen, which is not limited in this application. A control panel 93 is provided within the case 90 for operation.
In the embodiment of the present application, the main body of the magnetic stirrer 70 is disposed at the center inside the box 90, and substantially coincides with the projection of the first groove 11 in the vertical direction, so as to control the rotation of the stirrer inside the first groove 11. The control panel 93 is also disposed in the box 90, and an operation opening is formed in the box 90 to operate the control key.
The working principle of the photosynthetic bacteria reactor provided by the embodiment of the application is as follows:
the photosynthetic bacteria reactor is completely installed, whether each part can work normally is checked, the second valve is firstly closed to cut off the first drainage pipe 15, then the sealing cover 12 is opened, clear water is added into the first groove 11 for cleaning, then the second valve is opened, and the water in the first groove 11 is emptied through the first drainage pipe 15. And (3) switching on the power supply, pressing the second disinfection control key to start the second disinfection device 60 to sterilize and disinfect the first groove 11, and after disinfection is finished, pressing the second disinfection control key to close the second disinfection device 60.
The third valve is closed to shut off the second water discharge pipe 22, and water is filled into the annular groove 21 from the opening at the upper end of the annular groove 21 (which is beneficial to subsequent water bath heating and can balance the air pressure in the first groove 11 and the annular groove 21). Pressing first disinfection control key and second disinfection control key makes first degassing unit 61 and second degassing unit 60 all open, closes the second valve, makes first drain pipe 15 cut off the flow, adds the culture solution from inlet 53, in the culture solution gets into first recess 11 through disinfection pipe 52 and feed liquor pipe 51, sterilizes through first degassing unit 61 and second degassing unit 60, presses first disinfection control key and second disinfection control key to close first degassing unit 61 and second degassing unit 60.
Photosynthetic bacteria liquid is added from the liquid inlet 53, enters the first groove 11 through the disinfection pipe 52 and the liquid inlet pipe 51, and is inoculated to the nutrient solution. Pressing the stirring control key, and starting the magnetic stirrer 70 to control the stirring time and the stirring speed of the stirrer in the first groove 11; and the heating control key is pressed, the heating device 30 is started to heat water, so that the solution is heated in a water bath, and the temperature required by the culture of the photosynthetic bacteria is controlled; pressing the light control key turns on the light emitting device 40 to provide light to the photosynthetic bacteria.
After a certain number of days of cultivation, all switches are closed, the second valve is opened, the nutrient solution and the photosynthetic bacteria are discharged through the first drain pipe 15, and the cultivated photosynthetic bacteria are transferred into a designated container. The first groove 11 and the annular groove 21 are then cleaned.
The beneficial effects of the photosynthetic bacteria reactor that this application embodiment provided include:
(1) solution (nutrient solution + photosynthetic bacteria) through magnetic stirrers 70 in to first recess 11 stirs, need not use the mechanical stirring device who has the puddler to stir solution, magnetic stirrers 70 is when taking place to damage, the main part of direct change magnetic stirrers 70 can, can not cause the destruction to the anaerobic environment in first recess 11, in order to do benefit to photosynthetic bacteria's continuous culture, and the in-process of avoiding maintaining the agitator causes the pollution to photosynthetic bacteria's cultivation.
(2) The water bath heating provides the temperature required by the photosynthetic bacteria culture, the heating device 30 is prevented from contacting with the solution to pollute the solution, and the culture of the photosynthetic bacteria is prevented from being polluted. Meanwhile, the heating is more uniform in a water bath mode, and the growth of photosynthetic bacteria is facilitated.
(3) The nutrient solution is sterilized and disinfected by the first disinfection device 61 and the second disinfection device 60 respectively, so that the sterilization of the mixed bacteria in the nutrient solution is more thorough, and the introduction of the mixed bacteria is prevented from polluting photosynthetic bacteria. Meanwhile, the nutrient solution after the first sterilization and disinfection is directly discharged into the first groove 11, and no additional transfer is needed, so that the secondary pollution of the sterilized nutrient solution in the transfer process is avoided.
(4) And each power supply device is independently controlled through the control keys, so that the control of the conditions required by the growth of the photosynthetic bacteria is more accurate, and the growth of the photosynthetic bacteria is facilitated.
The above description is only a few examples of the present application and is not intended to limit the present application, and various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims (10)
1. A photosynthetic bacteria reactor, comprising:
the reaction device comprises an inner cylinder and an outer cylinder, wherein the inner cylinder is arranged in the outer cylinder and is provided with a first groove for culturing photosynthetic bacteria, and an annular groove for containing heat-conducting liquid is formed between the inner cylinder and the outer cylinder.
2. A photosynthetic bacteria reactor according to claim 1 wherein the inner vessel and the outer vessel are both transparent vessels, the walls of the outer vessel being provided with light emitting means.
3. A photosynthetic bacteria reactor according to claim 2 wherein the cylinder wall of the outer cylinder is provided with heating means configured to heat the thermally conductive liquid within the annular groove.
4. A photosynthetic bacteria reactor as claimed in claim 3, further comprising a liquid inlet device, wherein the liquid inlet device has a liquid inlet chamber therein, the liquid inlet chamber is communicated with the first groove, a first disinfection device for sterilization is disposed in the liquid inlet chamber, and a second disinfection device for sterilization is disposed in the first groove.
5. A photosynthetic bacteria reactor according to claim 4 wherein the first groove is provided with a detachable sealing cover, the liquid inlet device comprises a liquid inlet pipe and a disinfection pipe, the pipeline of the disinfection pipe and the pipeline of the liquid inlet pipe form the liquid inlet chamber, one end of the liquid inlet pipe is connected with the sealing cover and is communicated with the first groove, the other end of the liquid inlet pipe is communicated with the disinfection pipe, one end of the disinfection pipe, which is far away from the liquid inlet pipe, is provided with a liquid inlet, and the disinfection pipe is internally provided with the first disinfection device.
6. A photosynthetic bacteria reactor according to claim 5 wherein the disinfection tube is positioned above the sealing cover, the disinfection tube is horizontally disposed, and the liquid inlet tube is vertically disposed.
7. A photosynthetic bacteria reactor according to any one of claims 3-6 further comprising a magnetic stirrer, the bottom plate of the inner vessel being a glass plate;
the magnetic stirrer is arranged below the glass plate and is configured to form a magnetic field in the first groove so as to control the stirrer in the first groove to rotate.
8. A photosynthetic bacteria reactor according to claim 7 characterised in that the glass plate extends in the direction of the outer vessel and is sealingly connected to the lower end of the outer vessel, the outer wall of the inner vessel, the glass plate and the inner wall of the outer vessel forming the annular groove.
9. A photosynthetic bacteria reactor according to claim 8 further comprising a control means electrically connected to each of the magnetic stirrer, the heating means and the light emitting means.
10. A photosynthetic bacteria reactor according to claim 9 further comprising a control panel, wherein the control panel is provided with a power control key, a heating control key, a light emitting control key and a stirring control key, the power control key is electrically connected with the control device to control the control device to be powered on or powered off, the heating control key is electrically connected with the control device to control the heating device to be powered on or off, the light emitting control key is electrically connected with the control device to control the light emitting device to be powered on or off, and the stirring control key is electrically connected with the control device to control the magnetic stirrer to be powered on or off.
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CN202020498675.4U CN211947060U (en) | 2020-04-07 | 2020-04-07 | Photosynthetic bacteria reactor |
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CN202020498675.4U CN211947060U (en) | 2020-04-07 | 2020-04-07 | Photosynthetic bacteria reactor |
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