CN211856292U - Flow cytometer laser collimation calibrating device - Google Patents
Flow cytometer laser collimation calibrating device Download PDFInfo
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- CN211856292U CN211856292U CN201922357618.4U CN201922357618U CN211856292U CN 211856292 U CN211856292 U CN 211856292U CN 201922357618 U CN201922357618 U CN 201922357618U CN 211856292 U CN211856292 U CN 211856292U
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- 239000012530 fluid Substances 0.000 claims abstract description 19
- 238000007493 shaping process Methods 0.000 claims description 22
- 238000009434 installation Methods 0.000 abstract description 30
- 238000000034 method Methods 0.000 description 8
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
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Abstract
The utility model relates to a flow cytometer laser collimation calibrating device, belonging to the technical field of biological medical treatment; the laser collimator comprises a mounting base plate, a laser, a fluid focusing assembly, a reflecting mirror, a flow cell and two collimation sliding blocks, wherein a light path between the laser and the reflecting mirror is vertical to a light path between the reflecting mirror and the flow cell; the utility model provides a flow cytometer laser collimation calibrating device, its simple structure is reliable, and the installation is easy and simple to handle, and the precision is high.
Description
Technical Field
The utility model relates to a flow cytometer laser collimation calibrating device belongs to biomedical technical field.
Background
In the adjustment of the optical system of the current flow cytometer, an important link is to vertically center the laser beam on the flow channel of the flow cell, so as to realize the directional collection of forward and lateral fluorescence. The existing adjusting method has a difficulty that the laser is ensured to be vertically incident relative to the flow cell; this has a great influence on the accuracy of the subsequent image acquisition; therefore, the laser collimation needs to be calibrated, but the structure of the calibration device is complex;
various collimation methods utilize the characteristic that light rays are linearly transmitted, and a light through hole or a receiving point is arranged on a set path of the light; but the butt joint of the collimation tool and the optical assembly needs an angle square or a right-angle block to repeatedly determine the vertical or parallel reference relationship, which is very complicated; the axial collimation of the light beam is determined through the single light hole and the reciprocating movement; the technical requirements for workers are high. Certain error rates also exist for repeated confirmations.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to overcome not enough among the prior art, provide a flow cytometer laser collimation calibrating device, its simple structure is reliable, and the installation is easy and simple to handle, and the precision is high.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a flow cytometer laser collimation calibration device comprises a horizontal installation base plate, a laser arranged on the installation base plate, a fluid focusing assembly vertically arranged on the installation base plate, a reflecting mirror arranged between the fluid focusing assembly and the laser, a flow pool arranged above the fluid focusing assembly, a collimation guide rail arranged on the installation base plate and two collimation sliding blocks clamped on the collimation tool guide rail to slide, wherein a light path between the laser and the reflecting mirror is vertical to a light path between the reflecting mirror and the flow pool, the installation base plate is connected with the fluid focusing assembly and the collimation guide rail through a collimation fixing plate, the bottom surface of the collimation fixing plate is completely attached to the installation base plate, the bottom surface of the collimation guide rail is completely attached to the installation base plate, the fluid focusing assembly and the collimation guide rail are both attached to the side surface of the installation base plate, the side surface is vertical to the installation base plate and is parallel to a laser light path reflected by the reflecting mirror, the two collimation sliding blocks are vertically provided with through light holes, and laser reflected by the reflector penetrates through the flow cell and then sequentially penetrates through the two light holes and is parallel to the sliding direction of the collimation sliding blocks.
Further, the above-mentioned flow cytometer laser alignment calibration apparatus further includes a laser shaping device, where the laser shaping device includes a transverse shaping device and a longitudinal shaping device that are disposed on a light path between the reflector and the flow cell.
Furthermore, the reflecting mirror is connected with the mounting base plate in a sliding mode through a manual translation table, and the moving direction of the reflecting mirror is parallel to the light path between the laser and the reflecting mirror.
Further, the diameter of the light hole is not smaller than the cross-sectional diameter of the laser shaped by the laser shaping device.
Further, the two collimating sliders are identical.
Further, a laser supporting frame is further arranged between the installation bottom plate and the laser, the installation device supporting frame is connected with the installation bottom plate through bolts, and the laser is arranged above the laser supporting frame.
Compared with the prior art, the utility model discloses the beneficial effect who reaches:
1. the technical scheme is provided with the collimation fixing plate for mounting and positioning the mounting base plate, the fluid focusing assembly and the collimation guide rail, so that the positioning precision is ensured, and the mounting process is simple and convenient;
2. the technical scheme is provided with two identical collimation sliding blocks, so that errors caused by multiple sliding of the same sliding block are avoided, the operation is simplified, and the calibration precision is ensured;
3. the technical scheme is provided with the manual translation table, so that the reflector can be conveniently finely adjusted, the calibration precision is ensured, and the operation is simplified;
4. this technical scheme is equipped with laser shaping device and carries out the plastic to laser, conveniently obtains the facula of specific shape, satisfies the signal collection requirement.
Drawings
Fig. 1 is a schematic perspective view of a flow cytometer laser alignment calibration apparatus provided in an embodiment of the present invention (in the figure, a dotted line represents a laser path);
fig. 2 is a partially enlarged view at a in fig. 1 (the broken line in the drawing represents the laser light path).
Description of reference numerals: the method comprises the following steps of 1, mounting a base plate, 2, a laser, 3, a fluid focusing assembly, 4, a reflector, 5, a flow cell, 6, a collimation guide rail, 7, a collimation slide block, 8, a collimation fixing plate, 9, a light transmission hole, 10, a laser shaping device, 11, a transverse shaping device, 12, a longitudinal shaping device, 13, a manual translation table and 14, and a laser support frame.
Detailed Description
The present invention will be further described with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The attached drawing shows a laser collimation and calibration device for a flow cytometer, which comprises a horizontal installation base plate 1, a laser 2 arranged on the installation base plate 1, a fluid focusing assembly 3 vertically arranged on the installation base plate 1, a reflector 4 arranged between the fluid focusing assembly 3 and the laser 2, a flow cell 5 arranged above the fluid focusing assembly 3, a collimation guide rail 6 arranged on the installation base plate 1, and two collimation sliders 7 clamped on the collimation tool guide rail to slide, wherein a light path between the laser 2 and the reflector 4 is perpendicular to a light path between the reflector 4 and the flow cell 5, the installation base plate 1, the fluid focusing assembly 3 and the collimation guide rail 6 are connected through a collimation fixing plate 8, the bottom surface of the collimation fixing plate 8 is completely attached to the installation base plate 1, the bottom surface of the collimation guide rail 6 is completely attached to the installation base plate 1, the fluid focusing assembly 3 and the collimation guide rail 6 are both attached to the side surface of the installation base plate 1, the side surface is vertical to the mounting base plate 1 and is parallel to a laser path reflected by the reflector 4, the two collimating slide blocks 7 are vertically provided with through light holes 9, and laser reflected by the reflector 4 passes through the flow cell 5 and then sequentially passes through the two light holes and is parallel to the sliding direction of the collimating slide blocks 7;
in order to ensure that the incident light path and the reflected light path of the laser are mutually vertical, the mirror surface of the reflector 4 and the light path emitted by the laser 2 are arranged at an angle of 45 degrees, the collimation fixing plate 8, the fluid focusing assembly 3 and the collimation guide rail 6 are fixedly connected by utilizing the same side surface, and are fixedly connected by utilizing the installation bottom plate 1, and are positioned and fixed by sharing two mutually vertical surfaces, so that the uniformity of the installation positioning reference datum is ensured, the collimation fixing plate 8 is used, the straight guide rail 6 can be quickly positioned and installed, and the X, Z axis of the collimation guide rail 6 can be conveniently calibrated; can once only fix a position, simplify the installation step, guarantee installation positioning accuracy, avoid reusing angle square or right angle piece to remove the calibration guide rail.
Further, the device also comprises a laser shaping device 10, wherein the laser shaping device comprises a transverse shaping device 11 and a longitudinal shaping device 12 which are arranged on the light path between the reflecting mirror 4 and the flow cell 5;
the laser beam reflected by the reflector 4 irradiates the flow cell 5, and the laser passing through the flow cell 5 generates a forward signal, which is also a key channel of the flow cytometer; the laser beam needs to be shaped at the front end of the flow cell 5, so that a light spot with a specific shape is obtained after shaping, and the requirement of signal collection is met.
Further, the reflecting mirror 4 is connected with the installation bottom plate 1 in a sliding mode through a manual translation stage 13, and the moving direction of the reflecting mirror 4 is parallel to the light path between the laser 2 and the reflecting mirror 4;
the manual translation stage 13 facilitates fine adjustment of the light path reflected by the reflector 4 so as to vertically pass through the flow cell 5 and then pass through the light hole 9, thereby facilitating adjustment.
Further, the diameter of the light hole 9 is not less than the cross-sectional diameter of the laser after shaping by the shaping device;
the size of the light hole 9 is suitable, the laser after shaping can completely penetrate through the light hole and is not blocked, and errors caused by deflection of a light path which is difficult to detect due to the fact that the laser is too large cannot be met, the two collimating sliders 7 and the light hole 9 are calibrated at different distances, calibration precision is guaranteed, and calibration efficiency is improved.
Further, the two collimating sliders 7 are identical;
the two collimating slide blocks 7 are made of the same material, two workpieces with the same size are processed by the same process at the same time in the same environment, the positions of the two light holes 9 on the two workpieces are consistent, and the two light holes 9 are coaxial after being installed on the collimating guide rail 6, so that the calibration precision can be guaranteed, and the calibration efficiency is improved.
In one embodiment, a laser support frame 14 is further arranged between the installation bottom plate 1 and the laser 2, the installation device support frame is connected with the installation bottom plate 1 through bolts, and the laser 2 is arranged above a laser 2 support frame;
the mounting base plate 1 can be better protected as the connecting piece between 2 and the mounting base plate 1 of laser instrument 2 support frames, prevents that 2 direct contact of laser instrument and mounting base plate from causing wearing and tearing at the dismouting process, also can make 2 operation of laser instrument more steady.
The calibration method of the flow cytometer laser collimation calibration device comprises the following steps:
after the device is assembled, two collimation sliding blocks 7 are respectively arranged at two ends of the guide rail, and the position of the manual translation table is adjusted to enable laser to pass through two light holes 9 and to form the brightest and most concentrated light spot on a light screen about 2 meters away, wherein laser protective glasses are required to be worn in the above process to protect eyes;
only one collimation slider 7 is placed, whether the light beam is collimated or not can be judged by moving back and forth, but because only one collimation slider 7 is provided, the light spot on the light screen is shielded only once, the intensity change of the light spot is not obvious, and the light beam can be determined by adjusting and placing back and forth for several times; therefore, the two collimating sliders 7 can be used and operated simultaneously more conveniently, and the working hours for assembly and adjustment can be saved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be considered as the protection scope of the present invention.
Claims (6)
1. A flow cytometer laser collimation calibrating device which is characterized in that: the device comprises a horizontal mounting base plate (1), a laser (2) arranged on the mounting base plate, a fluid focusing assembly (3) vertically arranged on the mounting base plate, a reflecting mirror (4) arranged between the fluid focusing assembly and the laser, a flow pool (5) arranged above the fluid focusing assembly, a collimation guide rail (6) arranged on the mounting base plate and two collimation sliding blocks (7) clamped on the collimation tool guide rail, wherein the light path between the laser and the reflecting mirror is perpendicular to the light path between the reflecting mirror and the flow pool, the mounting base plate is connected with the fluid focusing assembly and the collimation guide rail through a collimation fixing plate (8), the bottom surface of the collimation fixing plate is completely attached with the mounting base plate, the bottom surface of the collimation guide rail is completely attached with the mounting base plate, and the fluid focusing assembly and the collimation guide rail are both attached with the side surface of the mounting base plate, the side surface is perpendicular to the mounting base plate and is parallel to a laser light path reflected by the reflector, the two collimation sliding blocks are provided with through light holes (9) vertically, and laser reflected by the reflector penetrates through the flow cell and then sequentially penetrates through the two light holes and is parallel to the sliding direction of the collimation sliding blocks.
2. A flow cytometer laser alignment calibration device as described in claim 1, wherein: the device also comprises a laser shaping device (10), wherein the laser shaping device comprises a transverse shaping device (11) and a longitudinal shaping device (12) which are arranged on a light path between the reflecting mirror and the flow cell.
3. A flow cytometer laser alignment calibration device as described in claim 1, wherein: the reflector is connected with the mounting base plate in a sliding mode through a manual translation table (13), and the moving direction of the reflector is parallel to a light path between the laser and the reflector.
4. A flow cytometer laser alignment calibration device as described in claim 2, wherein: the diameter of the light hole is not smaller than the cross-sectional diameter of the laser shaped by the laser shaping device.
5. A flow cytometer laser alignment calibration device as described in claim 1, wherein: the two collimating sliders are identical.
6. A flow cytometer laser alignment calibration device as described in claim 1, wherein: still be equipped with laser instrument support frame (14) between mounting plate and the laser instrument, pass through bolted connection between laser instrument support frame and the mounting plate, the laser instrument sets up in laser instrument support frame top.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922357618.4U CN211856292U (en) | 2019-12-25 | 2019-12-25 | Flow cytometer laser collimation calibrating device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922357618.4U CN211856292U (en) | 2019-12-25 | 2019-12-25 | Flow cytometer laser collimation calibrating device |
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| Publication Number | Publication Date |
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| CN211856292U true CN211856292U (en) | 2020-11-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201922357618.4U Active CN211856292U (en) | 2019-12-25 | 2019-12-25 | Flow cytometer laser collimation calibrating device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114894084A (en) * | 2022-02-16 | 2022-08-12 | 深圳市深视智能科技有限公司 | Laser transmitter calibration system and calibration method |
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2019
- 2019-12-25 CN CN201922357618.4U patent/CN211856292U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114894084A (en) * | 2022-02-16 | 2022-08-12 | 深圳市深视智能科技有限公司 | Laser transmitter calibration system and calibration method |
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| TR01 | Transfer of patent right |
Effective date of registration: 20231218 Address after: 230000, West Half Floor, 2nd Floor, Building 2, Phase II, Intelligent Science and Technology Park, No. 3959 Susong Road, Hefei Economic and Technological Development Zone, Anhui Province Patentee after: Zhongsheng Medical Technology (Hefei) Co.,Ltd. Address before: 215163 Room 101, building 3, No.8 Jinfeng Road, high tech Zone, Suzhou City, Jiangsu Province Patentee before: ZHONGSHENG (SUZHOU) MEDICAL TECHNOLOGY CO.,LTD. |