Sleeve type negative pressure biological reaction device
Technical Field
The utility model relates to a biochemical test reaction household utensils field specifically is a bushing type negative pressure biological reaction device.
Background
A bioreactor is a reaction system which utilizes naturally existing microorganisms or microorganisms with special degradation capacity to inoculate liquid phase or solid phase, is also used for experimental tools for culturing biological cell tissues, provides 1-hour high-pressure culture environment for the cell tissues every day, and has the advantages that strict aseptic conditions for culturing the cell tissues are required, once the polluted cell tissues are subjected to irrecoverable lethal shock, the asepsis is the basis of cell culture, the sufficient nutrient environment (including supply of nutrients (the degradation rate of biological factors required by various cells is high) and removal of waste) is realized, and the liquid is changed at regular intervals for meeting the requirements of the biological tissues.
Patent 2018112940730 discloses a biological culture reaction device, which comprises a culture chamber and a pressurizing assembly, wherein the pressurizing assembly comprises an inner pressure head, an upper pressure plate, a lower pressure plate and a pressure spring; the upper end face of the inner pressing head is connected with an upper pressing plate through a bolt, the lower end of the inner pressing head is sleeved with a lower pressing plate, the lower part of the inner pressing head is provided with a boss, the upper end of a through hole of the lower pressing plate is provided with a convex ring, and the lower pressing plate is matched with the boss at the lower part of the inner pressing head through the convex ring to be axially positioned, so that the lower pressing plate can move upwards along with the inner pressing; the inner pressure head is sleeved with a pressure spring, and two ends of the pressure spring are respectively in contact connection with the upper pressure plate and the lower pressure plate. The inner pressure head and the lower pressure plate are respectively in press-contact connection with the silica gel membrane in the culture cabin, wherein the lower pressure plate is used for tightly pressing the silica gel membrane and the upper end face of the tank body to tightly seal the silica gel membrane and the upper end face of the tank body, and the inner pressure head applies set pressure to liquid in the tank body through the silica gel membrane. The main technical problem solved by the technology of the patent is that the sealing condition is good, and the pollution of the reactor is reduced. But it is not very good to simulate the physical environment that cartilage in the joint cavity is subjected to under the environment of positive pressure alone.
At present, research shows that negative pressure can promote cell growth, but no negative pressure bioreactor exists in the market, and human joints and other parts exist under negative pressure for a long time, so the negative pressure bioreactor is also a bionic technology.
SUMMERY OF THE UTILITY MODEL
The utility model discloses the technical problem who mainly solves how to provide a bushing type negative pressure biological reaction device and method, can simulate the condition that joint motion and resting state negative pressure exist in the joint chamber, better deposit cartilage formation and promote the directional differentiation of cartilage.
In order to solve the technical problem, the utility model discloses a technical scheme as follows:
a sleeve type negative pressure biological reaction device is characterized in that: the structure comprises a culture tank body, wherein a tank cover is arranged on the culture tank body, a rubber diaphragm is tightly connected between the tank cover and the tank body, and a base plate is arranged between the tank cover and the rubber diaphragm, so that the stress is uniform during sealing; in order to increase the stability of the culture tank during placement, a fixed small arm is arranged at the bottom of the culture tank body, and the fixed small arm and the fixed notch are mutually clamped and rotated by about 5 degrees and are screwed tightly to be fixed with the culture tank body; the middle of the inner side of the culture tank body is provided with a supporting rod, a sleeve is movably mounted on the supporting rod, the sleeve is connected with a first ejector rod arranged at an opening on one side of the culture tank body, the first ejector rod is connected with a second ejector rod through a connecting piece, a sealing gasket is arranged on a part, extending into the wall of the culture tank body, of the second ejector rod, the tail end of the second ejector rod is connected with a pressurizing assembly, the pressurizing assembly acts on the tail end of the second ejector rod, the first ejector rod is acted through force transmission, the sleeve is jacked up to a rubber diaphragm.
Preferably, the bottom of the culture tank body is screwed and connected with the pressure sensor through a threaded opening.
Preferably, the pressurizing sleeve is connected with the air cylinder, the oil cylinder and the servo motor or the linear motor; when the device works, the sleeve is jacked up by the first ejector rod to generate corresponding negative pressure, after negative pressure stimulation is completed, the pressurizing assembly relaxes, and the sleeve and the ejector rod return to the initial state.
The utility model discloses beneficial effect:
the utility model discloses simple structure, and the negative pressure state of the human articular cavity of simulation that can be fine, the negative pressure reaches human articular cavity negative pressure-0.17- — 0.6bar in addition, and fixed bioreactor of putting into every day is cultivateed when cultivateing cartilage, the internal true environment of better simulation to better promote cartilage to form.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained without inventive work, wherein:
FIG. 1 is a schematic view of the connection structure of the reactor and the pressurizing sleeve of the present invention;
reference numerals:
1-a culture tank body; 2-can cover; 3-a rubber diaphragm; 4-fixing the forearm; 5-fixing the notch; 6-supporting rods; 7-a sleeve; 8-a first ejector rod; 9-ejector rod II; 10-a seal gasket; 11-pressure sensor.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it should be apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1, in an embodiment of the present invention, a sleeve-type negative pressure bioreactor is characterized in that: the structure comprises a culture tank body 1, wherein a tank cover 2 is arranged on the culture tank body 1, a rubber diaphragm 3 is tightly connected between the tank cover 2 and the tank body 1, and a base plate is arranged between the tank cover 2 and the rubber diaphragm 3, so that the stress is uniform during sealing; in order to increase the stability of the culture tank during placement, the bottom of the culture tank body 1 is provided with a small fixed arm 4, and the small fixed arm 4 and the fixed notch 5 are mutually clamped and rotated by about 5 degrees and are screwed down to be fixed with the culture tank body; the middle of the culture tank body 1 is internally provided with a support rod 6, the support rod is movably provided with a sleeve 7, the sleeve 7 is connected with a first ejector rod 8 arranged at an opening at one side of the culture tank body 1, the first ejector rod 8 is connected with a second ejector rod 9 through a connecting piece, a sealing gasket 10 is arranged at a part of the second ejector rod 9 extending into the wall of the culture tank body 1, the tail end of the second ejector rod 9 is connected with a pressurizing assembly, external force is applied to the first ejector rod 8 through the lever principle, the sleeve 7 jacks up the rubber diaphragm 3 through the first ejector rod 8, and corresponding negative pressure.
In the specific implementation process, the bottom of the culture tank body 1 is screwed with the pressure sensor 11 through a threaded opening.
In the specific implementation process, the pressurizing sleeve is connected with the air cylinder, the oil cylinder and the servo motor or the linear motor; under normal conditions, the upper end of the sleeve is flush with the bottom surface of the silicon membrane, and when the device works, external force is applied to the first 8 ejector rods according to the lever principle, the sleeve 7 jacks up the rubber membrane 3 through the first 8 ejector rods, and corresponding negative pressure is generated.
In the specific implementation process, the reaction method comprises the following steps:
step 1: putting the culture into the tank body of the utility model, adding corresponding culture solution, covering a matched culture dish cover to prevent pollution while oxygen and carbon dioxide enter, putting the culture dish into a cell culture box for normal culture according to the principle of the original culture dish;
step 2: carrying out negative pressure stimulation within a fixed time every day, taking out the culture tank body from the incubator before carrying out the negative pressure stimulation, taking down the culture dish cover, covering a sterilized silica gel membrane and covering the tank cover, and then screwing down by using a coaxial wrench matched with the tank cover;
and step 3: then putting the culture tank into a pressurizing reaction box, inserting the two tail ends of the ejector rods into preset positions in a pressurizing assembly, and fixing the culture tank;
and 4, step 4: opening the programmed pressurizing device, pressurizing the ejector rod II through the pressurizing assembly to drive the ejector rod I and the sleeve to move upwards, ejecting the silica gel membrane on the sleeve through the lever principle to generate negative pressure, then loosening the pressurizing device, and repeating the steps;
and 5: and after the negative pressure stimulation is completed, taking down the culture tank body, unscrewing the tank cover, taking down the tank cover and the diaphragm in a fume hood, replacing the culture dish cover, putting the culture dish cover into the cell culture box, and performing conventional culture again to complete the negative pressure biological reaction.
In a specific implementation process, the temperature in the pressurized reaction box in the step 3 is set to be constant at 37 ℃.
The utility model discloses beneficial effect:
the utility model discloses simple structure, and the negative pressure state of the human articular cavity of simulation that can be fine, the negative pressure reaches human articular cavity negative pressure-0.17- — 0.6bar in addition, and fixed bioreactor of putting into every day is cultivateed when cultivateing cartilage, the internal true environment of better simulation to better promote cartilage to form.
The above only is the embodiment of the present invention, not limiting the patent scope of the present invention, all of which utilize the equivalent structure or equivalent flow transformation made by the content of the specification of the present invention, or directly or indirectly applied to other related technical fields, all included in the same way in the patent protection scope of the present invention.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.