CN211705395U - Hydrogen peroxide instantaneous sterilization device - Google Patents
Hydrogen peroxide instantaneous sterilization device Download PDFInfo
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- CN211705395U CN211705395U CN201922497854.6U CN201922497854U CN211705395U CN 211705395 U CN211705395 U CN 211705395U CN 201922497854 U CN201922497854 U CN 201922497854U CN 211705395 U CN211705395 U CN 211705395U
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Abstract
The utility model discloses an instantaneous hydrogen peroxide sterilization device, which aims to solve the problem of slow sterilization speed of the sterilization device. The utility model discloses an including compressed air filter, the vaporization section of thick bamboo, the sterilization pipe, hydrogen peroxide liquid storage bottle, installation heating rod and evaporating dish in the vaporization section of thick bamboo, intercommunication liquid feed pipe between hydrogen peroxide liquid storage bottle and the vaporization section of thick bamboo, install the transfer pump on the liquid feed pipe, intercommunication intake pipe between compressed air filter and the vaporization section of thick bamboo, install the admission valve in the intake pipe, air-vent valve and flowmeter, intercommunication sterilization air-feed pipe between sterilization pipe and the vaporization section of thick bamboo, install the sterilization blast valve on the sterilization blast pipe, intercommunication concentration detection pipe on the sterilization pipe, installation concentration valve and hydrogen peroxide concentration sensor on the concentration detection pipe, intercommunication exhaust pipe between intake pipe and the sterilization air-feed pipe, the last residual valve of installation of exhaust pipe.
Description
Technical Field
The utility model relates to a sterilization apparatus, more specifically say, it relates to a hydrogen peroxide instantaneous sterilization apparatus.
Background
With the recent rise of CAR-T cell immunotherapy, there is a demand for corresponding immunotherapeutic cell preparation devices. A typical CAR-T course of therapy includes five steps: isolation, modification (CAR-T cell preparation), expansion, reinfusion and monitoring. In order to increase the efficiency and level of automation of cell preparation, cell therapy workstations (cell production isolators) have come into existence. Wherein the operations of cell separation, modification and the like are completed in the cell production isolator. When the cell-filled embryo sac and the cryopreservation tube (cell raw materials for short) enter the cell isolator, sterile transfer needs to be realized, and a special sterilizer and a transfer channel need to be developed. The process requirements of cell production need to realize rapid sterilization and rapid transfer of cell raw materials, corresponding sterilization equipment is needed, the traditional sterilizer is low in sterilization speed, the sterilization period is generally 3-5 hours, the shortest sterilization period is more than 30 minutes, and the requirement for rapid sterilization cannot be met.
SUMMERY OF THE UTILITY MODEL
The utility model overcomes the defect of slow sterilization speed of the existing sterilization device, provides a hydrogen peroxide instantaneous sterilization device, and has the advantages of high sterilization speed and good sterilization effect.
In order to solve the technical problem, the utility model discloses a following technical scheme: the utility model provides an instantaneous sterilization apparatus of hydrogen peroxide, including compressed air filter, the vaporization section of thick bamboo, the sterilization pipe, hydrogen peroxide stock solution bottle, install heating rod and evaporating dish in the vaporization section of thick bamboo, intercommunication liquid feed pipe between hydrogen peroxide stock solution bottle and the vaporization section of thick bamboo, install the transfer pump on the liquid feed pipe, intercommunication intake pipe between compressed air filter and the vaporization section of thick bamboo, install admission valve, air-vent valve and flowmeter in the intake pipe, intercommunication sterilization air supply pipe between sterilization pipe and the vaporization section of thick bamboo, install the sterilization air supply valve on the sterilization air supply pipe, intercommunication blast pipe on the sterilization pipe, install discharge valve on the blast pipe, intercommunication concentration detection pipe between blast pipe and the sterilization air supply pipe, install concentration valve and hydrogen peroxide concentration sensor on the concentration detection pipe, intercommunication residue discharge pipe between intake pipe and the sterilization air supply pipe, residue discharge valve is installed on the residue.
During the sterilization operation, the method comprises the following steps: a. installing a sterilizing tube on an isolator, placing the outlet end of the sterilizing tube in the isolator, placing the inlet end of the sterilizing tube outside the isolator, and placing a sterilizing object into the sterilizing tube from the outer end of the sterilizing tube; b. in the dehumidification stage, an air inlet valve, a pressure regulating valve, a sterilization air supply valve, an exhaust valve and a concentration valve are opened, a heating rod and an evaporation disc work for preheating, and the interior of the sterilization pipe is dehumidified to be below 10% RH; when the temperature in the sterilizing tube reaches 37 ℃, all valves are closed, hydrogen peroxide liquid drops are injected into the sterilizing tube through a delivery pump after the temperature on an evaporation disc reaches 140 ℃, an air inlet valve and a concentration valve are opened, the sterilizing tube enters a concentration adjusting stage, dry compressed air enters the vaporizing tube through a compressed air filter and a pressure regulating valve in the process, the pipeline is preheated through hot air in the vaporizing tube, and hydrogen peroxide gas is prevented from contacting with the tube wall and condensing into liquid hydrogen peroxide in the sterilizing process; c. setting the injection amount of hydrogen peroxide, starting a delivery pump to inject a hydrogen peroxide solution into a vaporization cylinder to vaporize the hydrogen peroxide solution to generate gaseous hydrogen peroxide, mixing the vaporized hydrogen peroxide gas with air, passing through a concentration valve, detecting the concentration by a hydrogen peroxide concentration sensor, and then discharging into an exhaust pipe, detecting the concentration of the hydrogen peroxide in real time by the hydrogen peroxide concentration sensor, and closing the concentration valve to enter a sterilization stage when the concentration reaches 3000 ppm; d. in the sterilization stage, a sterilization air supply valve and an exhaust valve are opened, hydrogen peroxide gas is introduced into a sterilization pipe, and the sterilization action time is 20 to 60 seconds; e. and a residue removing stage: and closing the sterilizing air supply valve, opening the residue discharge valve, the air inlet valve, the pressure regulating valve, the concentration valve and the exhaust valve, discharging residual hydrogen peroxide gas in the sterilizing tube and the vaporization cylinder by using dry compressed air sent by the compressed air filter, closing all the valves when the concentration of the hydrogen peroxide is less than 1ppm, finishing residue discharge, and taking out the sterilized substances from the isolator through the outlet end of the sterilizing tube. The whole sterilization process is fast and the sterilization effect is good.
Preferably, a temperature sensor is installed in the vaporization cylinder. The temperature sensor is convenient for controlling the temperature.
Preferably, a sterilization temperature sensor and a hydrogen peroxide sterilization concentration sensor are installed in the sterilization tube. The arrangement is convenient for controlling the temperature and the concentration of the hydrogen peroxide gas in the sterilizing tube, and ensures the sterilizing effect.
Preferably, the air intake valve, the residual air exhaust valve, the sterilization air supply valve, the exhaust valve, and the concentration valve are all solenoid valves. The control of the electromagnetic valve is convenient and reliable.
Preferably, the two ends of the sterilizing tube are respectively connected with an inlet end cover and an outlet end cover, and a flange is arranged on the side, close to the outlet end cover, of the sterilizing tube. The flange facilitates the connection of the sterilizing tube and the isolator. The inlet end cover and the outlet end cover ensure the sealing of the two ends of the sterilizing tube.
Preferably, the sterilizing tube is provided with an inductive switch near the inlet end cover, and the inlet end cover and the inductive switch are correspondingly provided with an inductive head. The arrangement of the inductive switch and the inductive head is convenient for judging whether the inlet end cover is connected in place.
Preferably, the device also comprises a mounting box, and the compressed air filter, the vaporizing cylinder, the hydrogen peroxide storage bottle, the infusion pump, the air inlet valve, the pressure regulating valve, the sterilization air supply valve, the exhaust valve, the concentration valve and the residue discharge valve are all mounted in the mounting box. The parts are arranged in the mounting box, so that the structure is compact, and the device is convenient to use.
Compared with the prior art, the beneficial effects of the utility model are that: when the hydrogen peroxide instantaneous sterilization device carries out sterilization operation, the sterilization speed is high, and the sterilization effect is good.
Drawings
FIG. 1 is a schematic diagram of the present invention;
fig. 2 is a schematic structural view of the usage state of the present invention;
fig. 3 is a partial structural schematic diagram of the present invention;
FIG. 4 is a schematic structural view of the sterilization tube of the present invention;
in the figure: 1. the device comprises a mounting box, 2, a compressed air filter, 3, a vaporization barrel, 4, a sterilization pipe, 5, a hydrogen peroxide liquid storage bottle, 6, a heating rod, 7, an evaporation disc, 8, a liquid feeding pipe, 9, an infusion pump, 10, an air inlet pipe, 11, an air inlet valve, 12, a pressure regulating valve, 13, a sterilization air feeding pipe, 14, a sterilization air feeding valve, 15, an exhaust pipe, 16, an exhaust valve, 17, a concentration detection pipe, 18, a concentration valve, 19, a hydrogen peroxide concentration sensor, 20, a residue discharge pipe, 21, a residue discharge valve, 22, a flowmeter, 23, a one-way valve, 24, a temperature sensor, 25, a sterilization temperature sensor, 26, a hydrogen peroxide sterilization concentration sensor, 27, an inlet end cover, 28, an outlet end cover, 29, a flange, 30, a sensing switch, 31, a sensing head, 32, an air inlet, 33 and an air.
Detailed Description
The technical solution of the present invention is further described in detail by the following specific embodiments in combination with the accompanying drawings: example (b): an instantaneous hydrogen peroxide sterilization device (see attached figures 1 to 4) comprises a mounting box 1, a compressed air filter 2, a vaporization cylinder 3, a sterilization pipe 4 and a hydrogen peroxide liquid storage bottle 5, wherein a heating rod 6 and an evaporation tray 7 are mounted in the vaporization cylinder, a liquid feeding pipe 8 is communicated between the hydrogen peroxide liquid storage bottle and the vaporization cylinder, a liquid conveying pump 9 is mounted on the liquid feeding pipe, an air inlet pipe 10 is communicated between the compressed air filter and the vaporization cylinder, an air inlet valve 11 and a pressure regulating valve 12 are mounted on the air inlet pipe, a sterilization air feeding pipe 13 is communicated between the sterilization pipe and the vaporization cylinder, a sterilization air feeding valve 14 is mounted on the sterilization air feeding pipe, an exhaust pipe 15 is communicated on the sterilization pipe, an exhaust valve 16 is mounted on the exhaust pipe, a concentration detection pipe 17 is communicated between the exhaust pipe and the sterilization air feeding pipe, a concentration valve 18 and a hydrogen peroxide concentration sensor 19 are mounted on the concentration detection pipe, the residue discharge pipe is provided with a residue discharge valve 21. The air inlet valve, the residual discharge valve, the sterilization air supply valve, the exhaust valve and the concentration valve are all electromagnetic valves. A flow meter 22 is mounted on the intake pipe. The infusion pump is a peristaltic pump, and a check valve 23 is arranged on the liquid feeding pipe. One end of the concentration detecting pipe is connected with the sterilizing air supply pipe between the sterilizing air supply valve and the vaporizing cylinder. One end of the residue discharge pipe is connected with the sterilization air supply pipe between the sterilization pipe and the sterilization air supply valve, and the other end of the residue discharge pipe is connected with the air inlet pipe between the compressed air filter and the air inlet valve. A temperature sensor 24 is arranged in the vaporization cylinder, the evaporation tray and the heating rods are arranged at the bottom of the vaporization cylinder, a plurality of heating rods are uniformly distributed, and the temperature sensor is arranged at the position of the evaporation tray. A sterilization temperature sensor 25 and a hydrogen peroxide sterilization concentration sensor 26 are installed in the sterilization tube. The two ends of the sterilizing tube are respectively connected with an inlet end cover 27 and an outlet end cover 28, and a flange 29 is arranged on the side of the sterilizing tube close to the outlet end cover. An induction switch 30 is arranged on the sterilizing tube close to the opening end cover, and an induction head 31 is correspondingly arranged on the outlet end cover and the induction switch. The sterilizing pipe is provided with an air inlet 32 and an air outlet 33, the air inlet and the air outlet are both arranged between the flange and the inlet end cover, the air inlet is connected with the sterilizing air supply pipe, and the air outlet is connected with the exhaust pipe. The compressed air filter, the vaporization cylinder, the hydrogen peroxide storage bottle, the infusion pump, the flowmeter, the air inlet valve, the pressure regulating valve, the sterilization air supply valve, the exhaust valve, the concentration valve and the residue discharge valve are all arranged in the mounting box.
When the hydrogen peroxide instantaneous sterilization device is used for sterilization, the method comprises the following steps: a. transversely installing a sterilizing pipe on an isolator, arranging an outlet end of the sterilizing pipe connected with an outlet end cover in the isolator, arranging an inlet end of a connecting inlet end cover outside the isolator, tightly connecting a flange with the isolator, opening the inlet end cover, putting a sterilizing object into the sterilizing pipe from the outer end of the sterilizing pipe, and closing the inlet end cover; b. in the dehumidification stage, an air inlet valve, a pressure regulating valve, a sterilization air supply valve, an exhaust valve and a concentration valve are opened, a heating rod and an evaporation disc work for preheating, and the interior of the sterilization pipe is dehumidified to be below 10% RH; when the temperature in the sterilizing tube reaches 37 ℃, all valves are closed, hydrogen peroxide liquid drops are injected into the sterilizing tube through a delivery pump after the temperature on an evaporation disc reaches 140 ℃, an air inlet valve and a concentration valve are opened, the sterilizing tube enters a concentration adjusting stage, dry compressed air enters the vaporizing tube through a compressed air filter and a pressure regulating valve in the process, the pipeline is preheated through hot air in the vaporizing tube, and hydrogen peroxide gas is prevented from contacting with the tube wall and condensing into liquid hydrogen peroxide in the sterilizing process; c. setting the injection amount of hydrogen peroxide, starting a delivery pump to inject a hydrogen peroxide solution into a vaporization cylinder to vaporize the hydrogen peroxide solution to generate gaseous hydrogen peroxide, mixing the vaporized hydrogen peroxide gas with air, passing through a concentration valve, detecting the concentration by a hydrogen peroxide concentration sensor, and then discharging into an exhaust pipe, detecting the concentration of the hydrogen peroxide in real time by the hydrogen peroxide concentration sensor, and closing the concentration valve to enter a sterilization stage when the concentration reaches 3000 ppm; d. in the sterilization stage, a sterilization air supply valve and an exhaust valve are opened, hydrogen peroxide gas is introduced into a sterilization pipe, and the sterilization action time is 20 to 60 seconds; e. and a residue removing stage: and closing the sterilizing air supply valve, opening the residue discharge valve, the air inlet valve, the pressure regulating valve, the concentration valve and the exhaust valve, discharging residual hydrogen peroxide gas in the sterilizing tube and the vaporization cylinder by using dry compressed air sent by the compressed air filter, closing all the valves when the concentration of the hydrogen peroxide is less than 1ppm, finishing residue discharge, and taking out the sterilized substances from the isolator through the outlet end of the sterilizing tube. The sterilizing tube can be arranged obliquely or vertically in the step a, and the arrangement mode needs to install a fixing frame for fixing the sterilizing object in the sterilizing tube to prevent the sterilizing object from sliding under the action of gravity.
The hydrogen peroxide instantaneous sterilization device performs the sterilization experiment as follows:
will 106Placing a Biological Indicator (BI) and a chemical indicator card into the sterilizing tube;
starting the hydrogen peroxide instantaneous sterilization device to perform sterilization operation;
and (3) quickly taking out the BI after the sterilization operation is finished, respectively inoculating the unsterilized BI and the sterilized BI to a TSB culture medium, and culturing for 7 days in a thermostat at 55-60 ℃.
The first set of experimental parameters was as follows:
| serial number | Humidity (RH%) | Sterilization time(s) | Sterilization concentration (ppm) | Temperature (. degree.C.) |
| 1 | 17.5-23 | 90 | 3323.2 | 41 |
| 2 | 17-20 | 60 | 3250 | 41.4 |
| 3 | 17-19 | 40 | 3323.4 | 41.5 |
| 4 | 18.5-20 | 20 | 2900 | 40 |
The experimental results are as follows:
the second set of experimental parameters was as follows:
| serial number | Humidity (RH%) | Sterilization time(s) | Sterilization concentration (ppm) | Temperature (. degree.C.) |
| 1 | 10-21 | 60 | 1800 | 37.6 |
| 2 | 9-22 | 60 | 1856.5 | 38.6 |
| 3 | 10-21.5 | 60 | 1500 | 37.3 |
| 4 | 11-23 | 60 | 1530 | 37.9 |
| 5 | 10.5-22.5 | 60 | 1572.5 | 36.4 |
The experimental results are as follows:
as can be seen from the results of the culture, Bacillus stearothermophilus 10 could be obtained at a high concentration of about 3000ppm for 20 seconds6The sterilization effect is achieved, the sterilization temperature is about 37 ℃, and the concentration can reach the thermophilic fat bacillus 10 when the concentration is 1500ppm6And (5) killing and sterilizing effects.
The above-described embodiments are merely preferred and are not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (7)
1. The utility model provides an instantaneous sterilization apparatus of hydrogen peroxide, characterized by, including compressed air filter, the vaporization section of thick bamboo, the sterilization pipe, hydrogen peroxide stock solution bottle, install heating rod and evaporating dish in the vaporization section of thick bamboo, communicate the liquid feed pipe between hydrogen peroxide stock solution bottle and the vaporization section of thick bamboo, install the transfer pump on the liquid feed pipe, communicate the intake pipe between compressed air filter and the vaporization section of thick bamboo, install admission valve, air-vent valve and flowmeter in the intake pipe, communicate the air feed pipe that disinfects between sterilization pipe and the vaporization section of thick bamboo, install the sterilization air feed valve on the sterilization air feed pipe, communicate the blast pipe on the sterilization pipe, install discharge valve on the blast pipe, communicate the concentration detection pipe between blast pipe and the sterilization air feed pipe, install concentration valve and hydrogen peroxide concentration sensor on the concentration detection pipe, communicate the exhaust pipe between intake pipe and the sterilization air feed pipe.
2. An instantaneous hydrogen peroxide sterilizer as claimed in claim 1, wherein the vaporizing cylinder is provided with a temperature sensor.
3. An instantaneous hydrogen peroxide sterilization apparatus as defined in claim 1, wherein a sterilization temperature sensor and a hydrogen peroxide sterilization concentration sensor are installed in the sterilization tube.
4. An instantaneous hydrogen peroxide sterilizer as claimed in claim 1, wherein the air inlet valve, residual discharge valve, sterilizing air supply valve, exhaust valve and concentration valve are all solenoid valves.
5. An instant hydrogen peroxide sterilizer as claimed in claim 1, wherein the sterilizing tube has an inlet end cap and an outlet end cap connected to both ends thereof, and a flange is provided on the sterilizing tube at a side close to the outlet end cap.
6. An instant hydrogen peroxide sterilizer as claimed in claim 5, wherein the sterilizing tube has an inductive switch near the inlet end cap, and an inductive head is mounted on the inlet end cap corresponding to the inductive switch.
7. The instantaneous hydrogen peroxide sterilization device according to any one of claims 1 to 6, further comprising a mounting box, wherein the compressed air filter, the vaporization barrel, the hydrogen peroxide storage bottle, the infusion pump, the air inlet valve, the pressure regulating valve, the sterilization air supply valve, the exhaust valve, the concentration valve and the residue discharge valve are all mounted in the mounting box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922497854.6U CN211705395U (en) | 2019-12-31 | 2019-12-31 | Hydrogen peroxide instantaneous sterilization device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922497854.6U CN211705395U (en) | 2019-12-31 | 2019-12-31 | Hydrogen peroxide instantaneous sterilization device |
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| Publication Number | Publication Date |
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| CN211705395U true CN211705395U (en) | 2020-10-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201922497854.6U Active CN211705395U (en) | 2019-12-31 | 2019-12-31 | Hydrogen peroxide instantaneous sterilization device |
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