CN102517204B - Cell gas poisoning apparatus - Google Patents
Cell gas poisoning apparatus Download PDFInfo
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- CN102517204B CN102517204B CN 201110432490 CN201110432490A CN102517204B CN 102517204 B CN102517204 B CN 102517204B CN 201110432490 CN201110432490 CN 201110432490 CN 201110432490 A CN201110432490 A CN 201110432490A CN 102517204 B CN102517204 B CN 102517204B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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Abstract
The invention discloses a cell gas poisoning apparatus comprising a cell poisoning cavity. A gas inlet of the cell poisoning cavity is connected to a gas mixing apparatus. A gas inlet of the gas mixing apparatus is connected to a gas outlet of a gas mixing apparatus. The cell poisoning cavity is connected to a gas discharging apparatus through a first gas outlet. A liquid inlet of the cell poisoning cavity is connected to a culture solution atomization supplying apparatus. The cell gas poisoning apparatus also comprises a temperature maintaining apparatus used for providing a constant-temperature poisoning environment for the cell poisoning cavity, and a controlling apparatus used for controlling a cell poisoning cavity temperature apparatus. According to the invention, under the controlling of the cell poisoning cavity temperature apparatus and the effect of the temperature maintaining apparatus, when the cell poisoning cavity reaches a preset temperature, living cells are placed in the cell poisoning cavity; oxygen from an oxygen generation apparatus and poisonous gas produced by a smoking machine are mixed in the gas mixing apparatus, wherein the oxygen satisfies a preset concentration requirement; the mixed gas is delivered to the cell poinsoning cavity through the first gas outlet, such that cell poisoning upon the living cells in the cell poisoning cavity is realized.
Description
Technical field
The present invention relates to a kind of laboratory apparatus, relate in particular to a kind of cell gas exposure instrument.
Background technology
Obnoxious flavour refers to diminish HUMAN HEALTH under general or certain condition, or the gas of harm job safety, includes poisonous gas, inflammable gas and choke damp etc., and obnoxious flavour is obvious to all to the harm of human body.Flue gas with cigarette is example, lung cancer is one of modal malignant tumour in the world, and studies show that at present smoking is one of major reason that causes lung cancer, also have a large amount of objectionable impuritiess in the cigarette, it produced murder by poisoning to the human body viable cell after these objectionable impuritiess were inhaled into human body, thereby brought out cell carcinogenesis.For the harm of more deep research obnoxious flavour to human body, need a kind of specific equipment that can carry out the cell contamination to obnoxious flavour at present badly.
Summary of the invention
The object of the present invention is to provide a kind of cell gas exposure instrument that can carry out the cell contamination to obnoxious flavour.
For achieving the above object, the invention provides a kind of cell gas exposure instrument, comprise cell contamination chamber, the inlet mouth in described cell contamination chamber is connected with gas mixer, the inlet mouth of described gas mixer links to each other with the air outlet of smoking machine, described cell contamination chamber is connected with gas barrier by first air outlet, the fluid inlet in described cell contamination chamber is connected with nutrient solution atomizing feedway, also comprise for the thermostat of the constant temperature toxic environment being provided for described cell contamination chamber, and the control device that is used for the control said apparatus.
Cell gas exposure instrument of the present invention comprises that also the escape pipe of described ozonizer is provided with first magnetic valve for the ozonizer of giving described cell contamination chamber sterilization.
Cell gas exposure instrument of the present invention, described cell contamination chamber, described gas mixer, described gas barrier, described nutrient solution atomizing feedway, described thermostat and described ozonizer are positioned at the clean work station of box shape.
Cell gas exposure instrument of the present invention, described cell contamination chamber is the capsule shape chamber, it comprises the last jet chamber of half capsule shape and the following nutrition chamber of half capsule shape that is mated, be equipped be used to the culture dish that holds viable cell by carriage in the nutrition chamber under described, described first inlet pipe that is provided with straight-through described culture dish in the jet chamber that, the upper end of described first inlet pipe protrudes upward the spherical upper end in described jet chamber and links to each other with the escape pipe of described gas mixer and the escape pipe of described ozonizer respectively by pipeline, described jet chamber is provided with first escape pipe at the position that is positioned at described first inlet pipe, one side, described first escape pipe links to each other with described gas barrier, described nutrition chamber down is provided with first liquid-inlet pipe and first drain pipe, described first liquid-inlet pipe links to each other with described nutrient solution atomizing feedway, the lower ends downward of described first drain pipe is stretched out the described spherical lower end in nutrition chamber down and is connected to a Y-tube, second passage of described Y-tube links to each other with the influx of described nutrient solution atomizing feedway and constitutes the closed cycle device, and the 3rd passage of described Y-tube stretches out outside the described clean work station by a bend pipe.
Cell gas exposure instrument of the present invention, described gas mixer comprises a gas mixer, described gas mixer is provided with for the oxygen intake pipe that links to each other with oxygen generating plant and at least one is for the toxic gas inlet pipe that links to each other with the toxic gas generating unit, one of them described toxic gas inlet pipe links to each other with the smoke outlet of described smoking machine, on described oxygen intake pipe and the described toxic gas inlet pipe respectively correspondence the 6th magnetic valve and the 7th magnetic valve are installed, be equipped with gas concentration sensor in described oxygen intake pipe and the described toxic gas inlet pipe, the outlet of described gas mixer with advance the plume control valve and link to each other, describedly advance the plume control valve and link to each other with described first inlet pipe by a pipe connecting, second magnetic valve is installed on the described pipe connecting.
Cell gas exposure instrument of the present invention, described gas barrier comprises off-gas pump, the inlet mouth of described off-gas pump links to each other with described first escape pipe by extraction flow control valve, the 3rd magnetic valve, the tar strainer of serial connection successively, and stretch out to the right outside the described clean work station air outlet of described off-gas pump.
Cell gas exposure instrument of the present invention, described nutrient solution atomizing feedway comprises ultrasonic humidifier, the escape pipe of described ultrasonic humidifier links to each other with described first liquid-inlet pipe, be provided with the 5th magnetic valve and pressure unit on the escape pipe of described ultrasonic humidifier successively, be provided with humidity sensor in described first liquid-inlet pipe, the inlet pipe of described ultrasonic humidifier links to each other with described threeway, and the inlet pipe of described ultrasonic humidifier is provided with the 4th magnetic valve.
Cell gas exposure instrument of the present invention, described thermostat comprises corresponding upper water box and the lower header that covers on described jet chamber and the described nutrition chamber down respectively, described upper water box and described lower header communicate, described upper water box links to each other with the electric heating constant temperature water tank, described electric heating constant temperature water tank links to each other with the suction port of water pump, and the relief outlet of described water pump links to each other with described lower header.
Cell gas exposure instrument of the present invention, described control device comprises:
Ozone air inlet control unit, it is successively by a switch triode and a solid state relay and described first magnetic valve, and the ozone that is used for the described ozonizer generation of control enters in the described cell contamination chamber;
Humidity controlling unit, it is successively by switch triode and a solid state relay and described the 5th magnetic valve, is used for entering described cell according to the Data Control nutrient solution that described humidity sensor and described pressure unit are passed back and contaminates in the chamber;
The oxygen control unit, it is successively by a switch triode and a solid state relay and described the 6th magnetic valve, and the Data Control oxygen that is used for passing back according to oxygen concentration sensor enters in the described gas mixer;
The smog control unit, it is successively by a switch triode and a solid state relay and described the 7th magnetic valve, and the Data Control flue gas that is used for passing back according to the flue gas concentration sensor enters in the described gas mixer;
The air inlet control unit, it is successively by switch triode and a solid state relay and described second magnetic valve, is used for advancing the plume control valve by described control and controls mixed gas and enter described cell and contaminate in the chamber;
The control unit of bleeding, it is successively by a switch triode and a solid state relay and described the 3rd magnetic valve, is used for discharging described cell by the gas after described extraction flow control valve and the described off-gas pump control test and contaminates outside the chamber;
Ozone is discharged control unit, and it is successively by a switch triode and a solid state relay and described the 4th magnetic valve, and the ozone that is used for after control is sterilized is discharged outside the described cell contamination chamber;
The constant temperature water tank control unit is used for the described thermostat of control and maintains temperature required;
Upper computer is used for carrying out communication with above-mentioned control unit.
Cell gas exposure instrument of the present invention is equipped with into cigarette strainer on the described pipeline that advances between plume control valve and described second magnetic valve, on the pipeline between described extraction flow control valve and the described water pump strainer of bleeding is installed.
Cell gas exposure instrument of the present invention comprises cell contamination chamber, the inlet mouth in cell contamination chamber is connected with gas mixer, the inlet mouth of gas mixer links to each other with the air outlet of smoking machine, cell contamination chamber is connected with gas barrier by first air outlet, the fluid inlet in cell contamination chamber is connected with nutrient solution atomizing feedway, also comprise for the thermostat that the constant temperature toxic environment is provided to cell contamination chamber, and the control device that is used for control device.During use, gas mixer is linked to each other with oxygen generating plant, under the control of control device, fixed temperature (is generally 37 ℃ when cell contamination chamber both is issued in the thermostat effect, and human body temperature) time, viable cell is put in the cell contamination chamber, the survival of viable cell is then ensured by the atomizing of nutrient solution then feedway, and then will (be generally 21% from set concentration of meeting of oxygen generating plant, being oxygen concentration in the atmosphere) toxic gas that produces of the oxygen that requires and smoking machine mixes the back at gas mixer and delivers to cell contamination chamber by first air outlet, thereby realized the viable cell that is put in the cell contamination chamber is carried out the cell contamination.
Description of drawings
Fig. 1 is the cell gas exposure instrument structural representation of one embodiment of the invention;
Fig. 2 is the electrical structure synoptic diagram of the control device part of cell gas exposure instrument among Fig. 1.
Embodiment
Below in conjunction with accompanying drawing the specific embodiment of the present invention is described in detail:
The cell gas exposure instrument of present embodiment comprises cell contamination chamber, the inlet mouth in cell contamination chamber is connected with gas mixer, the inlet mouth of gas mixer links to each other with the air outlet of smoking machine, cell contamination chamber is connected with gas barrier by first air outlet, the fluid inlet in cell contamination chamber is connected with nutrient solution atomizing feedway, also comprise for the thermostat that the constant temperature toxic environment is provided to cell contamination chamber, be used for the ozonizer to cell contamination chamber sterilization, and the control device that is used for these devices of control.
In conjunction with shown in Figure 1, gas mixer, gas barrier, nutrient solution atomizing feedway, thermostat and ozonizer 1 all are positioned at the clean work station 2 of box shape, to prevent external environmental.In addition, the escape pipe of ozonizer 1 is provided with the first magnetic valve J1 that enters cell contamination chamber for control ozone.
Wherein, cell contamination chamber is the capsule shape chamber, it comprises the last jet chamber 3 of half capsule shape and the following nutrition chamber 4 of half capsule shape that is mated, be equipped be used to the culture dish 5 that holds viable cell by carriage 8 in the following nutrition chamber 4, go up first inlet pipe that is provided with straight-through culture dish 5 in the jet chamber 3, the upper end of first inlet pipe protrudes upward the spherical upper end in jet chamber 3 and links to each other with the escape pipe of gas mixer 6 and the escape pipe of ozonizer 1 respectively by pipeline, go up jet chamber 3 and be provided with first escape pipe at the position that is positioned at first inlet pipe, one side, first escape pipe links to each other with tar strainer 10, following nutrition chamber 4 is provided with first liquid-inlet pipe and first drain pipe, first liquid-inlet pipe links to each other with the escape pipe of ultrasonic humidifier 11, the lower ends downward of first drain pipe is stretched out down, and the spherical lower end in nutrition chamber 4 is connected to a Y-tube, second passage of Y-tube links to each other with the influx of ultrasonic humidifier 11, thereby make ultrasonic humidifier 11 constitute the closed cycle device, the 3rd passage of Y-tube stretches out outside the clean work station 2 by a bend pipe.Wherein, carriage 8 is generally the carriage of a series of different heights, selects as required during use, with the lower end of first inlet pipe of adjusting jet chamber 3 and the distance between the culture dish 5, satisfies the different experiments requirement.
Wherein, gas mixer comprises a gas mixer 6, gas mixer 6 be provided with for the oxygen intake pipe that links to each other with oxygen generating plant (figure does not draw) with arrange as required one or more for the toxic gas inlet pipe that links to each other with the toxic gas generating unit, one of them toxic gas inlet pipe links to each other with the smoke outlet of smoking machine 7 (a kind of equipment that can extract smog from light back cigarette), when needs add other one or more toxic gases, the toxic gas inlet pipe of respective amount then is set, corresponding the 6th magnetic valve J6 and the 7th magnetic valve J7 that flows into for control gas that be equipped with respectively on oxygen intake pipe and the toxic gas inlet pipe, be equipped with gas concentration sensor in oxygen intake pipe and the toxic gas inlet pipe, to detect inlet gas concentration (gas concentration sensor does not draw among the figure), the outlet of gas mixer 6 with advance plume control valve V1 and link to each other, advance plume control valve V1 and link to each other with first inlet pipe by a pipe connecting, the second magnetic valve J2 that flows into for the control mixed gas is installed on the pipe connecting.Wherein, advance on the pipeline between plume control valve V1 and the second magnetic valve J2 into cigarette strainer M1 is installed.
Wherein, gas barrier comprises off-gas pump 9, and the inlet mouth of off-gas pump 9 links to each other with first escape pipe by extraction flow control valve V2, the 3rd magnetic valve J3, the tar strainer 10 of serial connection successively, and stretch out to the right outside the clean work station 2 air outlet of off-gas pump 9.Wherein, on the pipeline between extraction flow control valve V2 and the water pump 17 the strainer M2 that bleeds is installed.
Wherein, nutrient solution atomizing feedway comprises ultrasonic humidifier 11, be injected with nutritive medium in it during use, be provided with the 5th magnetic valve J5 on the escape pipe of ultrasonic humidifier 11 successively and for detection of the pressure unit 12 of cell contamination cavity pressure, be provided with the humidity sensor 13 for detection of humidity in the cell contamination chamber in first liquid-inlet pipe, the inlet pipe of ultrasonic humidifier 11 is provided with the 4th magnetic valve J4.
Wherein, thermostat comprises corresponding upper water box 14 and the lower header 15 that covers on jet chamber 3 and the following nutrition chamber 4 respectively, upper water box 14 and lower header 15 communicate, upper water box 14 links to each other with electric heating constant temperature water tank 16, electric heating constant temperature water tank 16 links to each other with the suction port of water pump 17, the relief outlet of water pump 17 links to each other with lower header 15, electric heating constant temperature water tank 16 produces 37 ℃ of constant warm water during use, 37 ℃ of warm water under the effect of water pump 17 at electric heating constant temperature water tank 16, lower header 15 and upper water box 14 internal recycle flow, thereby provide needed isoperibol for the cell that is positioned at upper water box 14 and lower header 15 chamber of contaminating.
In conjunction with shown in Figure 2, wherein, control device comprises ozone air inlet control unit, and it is successively by switch triode and solid state relay and the first magnetic valve J1, is used for ozone that control ozonizer 1 produces and enters cell and contaminate in the chamber; Humidity controlling unit, it is successively by switch triode and a solid state relay and described the 5th magnetic valve J5, is used for entering cell according to the Data Control nutrient solution that humidity sensor 13 and pressure unit 12 are passed back and contaminates in the chamber; The oxygen control unit, it by switch triode and solid state relay and the 6th magnetic valve J6, enters in the gas mixer 6 for the Data Control oxygen of passing back according to oxygen concentration sensor successively; The smog control unit, it by switch triode and solid state relay and the 7th magnetic valve J7, enters in the gas mixer 6 for the Data Control flue gas of passing back according to the flue gas concentration sensor successively; The air inlet control unit, it is successively by switch triode and solid state relay and the second magnetic valve J2, be used for advancing plume control valve V1 by control and control mixed gas and enter in the cell contamination chamber, wherein can manually control also can timing control for mixed gas the contamination process in the cell contamination chamber of entering; The control unit of bleeding, it is successively by a switch triode and solid state relay and the 3rd magnetic valve J3, is used for discharging cell by the gas after extraction flow control valve V2 and the off-gas pump 9 control tests and contaminates outside the chamber; Ozone is discharged control unit, and it is successively by a switch triode and solid state relay and the 4th magnetic valve J4, is used for ozone after the control sterilization and discharges cell and contaminate outside the chamber; The constant temperature water tank control unit is used for the control thermostat and maintains temperature required; Upper computer is used for carrying out communication with control unit.
Above embodiment is described preferred implementation of the present invention; be not that scope of the present invention is limited; design under the prerequisite of spirit not breaking away from the present invention; various distortion and improvement that the common engineering technical personnel in this area make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (7)
1. cell gas exposure instrument, it is characterized in that, comprise cell contamination chamber, the inlet mouth in described cell contamination chamber is connected with gas mixer, the inlet mouth of described gas mixer links to each other with the air outlet of smoking machine, described cell contamination chamber is connected with gas barrier by its air outlet, the fluid inlet in described cell contamination chamber is connected with nutrient solution atomizing feedway, also comprise for the thermostat of the constant temperature toxic environment being provided for described cell contamination chamber, and the control device that is used for the control said apparatus; Also comprise for the ozonizer (1) of giving described cell contamination chamber sterilization, the escape pipe of described ozonizer (1) is provided with first magnetic valve (J1), described cell contamination chamber, described gas mixer, described gas barrier, described nutrient solution atomizing feedway, described thermostat and described ozonizer (1) are positioned at the clean work station (2) of box shape, wherein:
Described cell contamination chamber is the capsule shape chamber, it comprises the last jet chamber (3) of half capsule shape and the following nutrition chamber (4) of half capsule shape that is mated, described nutrition chamber (4) down is interior to be equipped be used to the culture dish that holds viable cell (5) by carriage (8), described first inlet pipe that is provided with straight-through described culture dish (5) in the jet chamber (3) that, the upper end of described first inlet pipe protrudes upward the spherical upper end in described jet chamber (3) and links to each other with the escape pipe of described gas mixer and the escape pipe of described ozonizer (1) respectively by pipeline, the described jet chamber (3) of is provided with first escape pipe at the position that is positioned at described first inlet pipe, one side, described first escape pipe links to each other with described gas barrier, described nutrition chamber (4) down is provided with first liquid-inlet pipe and first drain pipe, described first liquid-inlet pipe links to each other with described nutrient solution atomizing feedway, the lower ends downward of described first drain pipe is stretched out the described spherical lower end in nutrition chamber (4) down and is connected to a Y-tube, second passage of described Y-tube links to each other with the influx of described nutrient solution atomizing feedway and constitutes the closed cycle device, and the 3rd passage of described Y-tube stretches out outside the described clean work station (2) by a bend pipe.
2. cell gas exposure instrument according to claim 1, it is characterized in that, described gas mixer comprises a gas mixer (6), described gas mixer (6) is provided with for the oxygen intake pipe that links to each other with oxygen generating plant and at least one is for the toxic gas inlet pipe that links to each other with the toxic gas generating unit, one of them described toxic gas inlet pipe links to each other with the smoke outlet of described smoking machine (7), on described oxygen intake pipe and the described toxic gas inlet pipe respectively correspondence the 6th magnetic valve (J6) and the 7th magnetic valve (J7) are installed, be equipped with gas concentration sensor in described oxygen intake pipe and the described toxic gas inlet pipe, the outlet of described gas mixer (6) with advance plume control valve (V1) and link to each other, describedly advance plume control valve (V1) and link to each other with described first inlet pipe by a pipe connecting, second magnetic valve (J2) is installed on the described pipe connecting.
3. cell gas exposure instrument according to claim 2, it is characterized in that, described gas barrier comprises off-gas pump (9), the inlet mouth of described off-gas pump (9) links to each other with described first escape pipe by extraction flow control valve (V2), the 3rd magnetic valve (J3), the tar strainer (10) of serial connection successively, and stretch out to the right outside the described clean work station (2) air outlet of described off-gas pump (9).
4. cell gas exposure instrument according to claim 3, it is characterized in that, described nutrient solution atomizing feedway comprises ultrasonic humidifier (11), the escape pipe of described ultrasonic humidifier (11) links to each other with described first liquid-inlet pipe, be provided with the 5th magnetic valve (J5) and pressure unit (12) on the escape pipe of described ultrasonic humidifier (11) successively, be provided with humidity sensor (13) in described first liquid-inlet pipe, the liquid-inlet pipe of described ultrasonic humidifier (11) links to each other with described Y-tube, and the liquid-inlet pipe of described ultrasonic humidifier (11) is provided with the 4th magnetic valve (J4).
5. cell gas exposure instrument according to claim 4, it is characterized in that, described thermostat comprises that correspondence covers on described upper water box (14) and the lower header of on jet chamber (3) and the described nutrition chamber (4) down (15) respectively, described upper water box (14) and described lower header (15) communicate, described upper water box (14) links to each other with electric heating constant temperature water tank (16), described electric heating constant temperature water tank (16) links to each other with the suction port of water pump (17), and the relief outlet of described water pump (17) links to each other with described lower header (15).
6. cell gas exposure instrument according to claim 5 is characterized in that described control device comprises:
Ozone air inlet control unit, it links to each other with described first magnetic valve (J1) with a solid state relay by a switch triode successively, and the ozone that is used for the described ozonizer of control (1) generation enters in the described cell contamination chamber;
Humidity controlling unit, it links to each other with described the 5th magnetic valve (J5) with a solid state relay by a switch triode successively, enters described cell for the Data Control nutrient solution of passing back according to described humidity sensor (13) and described pressure unit (12) and contaminates in the chamber;
The oxygen control unit, it links to each other with described the 6th magnetic valve (J6) with a solid state relay by a switch triode successively, and the Data Control oxygen that is used for passing back according to oxygen concentration sensor enters in the described gas mixer (6);
The smog control unit, it links to each other with described the 7th magnetic valve (J7) with a solid state relay by a switch triode successively, and the Data Control flue gas that is used for passing back according to the flue gas concentration sensor enters in the described gas mixer (6);
The air inlet control unit, it links to each other with described second magnetic valve (J2) with a solid state relay by a switch triode successively, is used for advancing plume control valve (V1) and controlling mixed gas and enter described cell and contaminate in the chamber by described;
The control unit of bleeding, it links to each other with described the 3rd magnetic valve (J3) with a solid state relay by a switch triode successively, is used for discharging outside the described cell contamination chamber by the gas after described extraction flow control valve (V2) and described off-gas pump (9) the control test;
Ozone is discharged control unit, and it links to each other with described the 4th magnetic valve (J4) with a solid state relay by a switch triode successively, and the ozone that is used for after control is sterilized is discharged outside the described cell contamination chamber;
The constant temperature water tank control unit is used for the described thermostat of control and maintains temperature required;
Upper computer is used for carrying out communication with above-mentioned control unit.
7. cell gas exposure instrument according to claim 6, it is characterized in that, on the described pipeline that advances between plume control valve (V1) and the described gas mixer (6) into cigarette strainer (M1) is installed, on the pipeline between described extraction flow control valve (V2) and the described off-gas pump (9) strainer of bleeding (M2) is installed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110432490 CN102517204B (en) | 2011-12-21 | 2011-12-21 | Cell gas poisoning apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110432490 CN102517204B (en) | 2011-12-21 | 2011-12-21 | Cell gas poisoning apparatus |
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| Publication Number | Publication Date |
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| CN102517204A CN102517204A (en) | 2012-06-27 |
| CN102517204B true CN102517204B (en) | 2013-09-11 |
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| CN 201110432490 Expired - Fee Related CN102517204B (en) | 2011-12-21 | 2011-12-21 | Cell gas poisoning apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106399076B (en) * | 2016-09-14 | 2018-10-02 | 南京大学 | Micro-fluidic gel gas-liquid interface fume exposure device |
| CN108212922B (en) * | 2018-02-11 | 2023-04-28 | 中铁建设集团有限公司 | Intelligent ultrasonic air conveying pipeline cleaning system and method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10158964A1 (en) * | 2001-11-30 | 2003-08-07 | Digi Plan Gmbh Planungsgesells | Detecting the presence of living cells and the like, in a solid, liquid or gas medium, comprises isolated cells from a sample, cultivating them in a chamber under light, and taking optical images from a sensor for evaluation |
| CN102168012B (en) * | 2011-01-08 | 2013-08-14 | 中国烟草总公司郑州烟草研究院 | Toxicity contamination testing device suitable for adding harmful components under full smoke gas toxicity contamination way of cigarettes |
| CN202054824U (en) * | 2011-03-11 | 2011-11-30 | 云南烟草科学研究院 | Device for whole cigarette smoke exposure |
| CN102191176B (en) * | 2011-03-28 | 2013-03-20 | 天津开发区合普工贸有限公司 | Experimental device for cell exposure contamination with combination of radon gas and cigarette smoke |
| CN102212469A (en) * | 2011-04-14 | 2011-10-12 | 苏州大学 | Cell radon gas and benzene combined exposure poisoning test equipment |
| CN202430221U (en) * | 2011-12-21 | 2012-09-12 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Cell gas contamination device |
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