CN106399076B - Micro-fluidic gel gas-liquid interface fume exposure device - Google Patents
Micro-fluidic gel gas-liquid interface fume exposure device Download PDFInfo
- Publication number
- CN106399076B CN106399076B CN201610826876.0A CN201610826876A CN106399076B CN 106399076 B CN106399076 B CN 106399076B CN 201610826876 A CN201610826876 A CN 201610826876A CN 106399076 B CN106399076 B CN 106399076B
- Authority
- CN
- China
- Prior art keywords
- gel
- cell
- gas
- layer chip
- liquid interface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
Abstract
The invention discloses a kind of micro-fluidic gel gas-liquid interface fume exposure device, including the upper layer chip of flue gas access way is provided, the lower layer chip in cell culture solution flow channel is provided and between levels chip for load gel and on gel cell middle layer chip；Wherein, the cell culture fluid in lower layer chip provides living environment for the cell in middle layer chip, and the gel in middle layer chip becomes gas-liquid interface and its cell is made to be in direct contact with flue gas.The present invention uses the combination of three layers of chip, cell to be located above gel, be not in direct contact with cell culture fluid, avoid culture solution and cause directly to impact to cell surface；Culture solution can be supplied cell by gel and keep cells survival ambient stable again simultaneously；Cell can be in direct contact with flue gas on this basis, to obtain related biological information of the flue gas to cytositimulation.
Description
Technical field
The present invention relates to a kind of fume exposure more particularly to a kind of micro-fluidic gel gas-liquid interface fume exposure devices.
Background technology
All the time, cigarette smoke is widely noticed the health risk of human body.Studies have reported that display, smoker relative to
Non-smoker, suffering from lung cancer, chronic obstructive pulmonary disease and the possibility of acute myocardial infarction can increase to some extent.Research shows that
In a variety of smoking relevant diseases caused by cigarette smoke, oxidative stress is the pathophysiological mechanism of key.Cigarette smoke is
Active oxygen (ROS) increases in a kind of mixture of complexity, wherein oxidizing substance meeting inducing cell, and cigarette smoke induces body production
Raw ROS can cause to damage to intracellular protein, lipid and DNA etc..But existing Evaluation Platform to its formation mechenism, at
Limitation is all compared in the researchs such as point harmfulness.Therefore, exploitation is to the in vitro toxicity research platform of cell, tool of the assessment flue gas to health
Body harmfulness, it is necessary to.
Cigarette smoke is a kind of complicated aerosol being made of thousands of kinds of chemical substances, by granule phase substance and gas gas-phase objects two
Divide and is constituted.Cigarette smoke toxicologic study is mostly focused on the toxicity research aspect of Smoke Particulate at present, and contaminating mode is more
Based on exposing with solution, there is huge difference with the actual respiratory tract gas-liquid interface exposure condition of cigarette smoke, it cannot be comprehensive
Truly reflect the biological effect of smoke mixture system.And research now directly exposed to full flue gas, mostly use commodity
The Vitrocell of the Cultex and Germany such as Japan are set in makeup, both equipment are essentially identical, and exposure chamber is gas-liquid interface
Exposure.But such exposing device is bulky, expensive, common lab is difficult to bear, and limits pushing away for such device
It is wide and universal.In addition, characteristic of the cigarette due to its natural products, there is obvious difference between different sources and different batches
It is different, therefore need a large amount of work, existing equipment to be difficult to meet the basic need of toxicity evaluation completely the assessment of its toxicity
It asks.Therefore, it is badly in need of establishing the simple and easy gas-liquid interface cell exposure platform that a kind of laboratory uses and is commented for gas toxicity.
Invention content
Goal of the invention：The object of the present invention is to provide a kind of fume exposures for laboratory research, portable and cheap
Device.
Technical solution：Micro-fluidic gel gas-liquid interface fume exposure device of the present invention, including flue gas disengaging is provided
The upper layer chip in channel, the lower layer chip that cell culture solution flow channel is provided and between levels chip for loading
Gel and on gel cell middle layer chip；Wherein, the cell culture fluid in lower layer chip is in middle layer chip
Cell provides living environment, and the gel in middle layer chip becomes gas-liquid interface and its cell is made to be in direct contact with flue gas.
Wherein, the upper layer chip includes the gas access way being set up in parallel and the gas access positioned at channel both sides
And gas vent, wherein gas access at least there are two, there is no limit such as two entrances are passed through two kinds to gas vent quantity
Different gas, mutually dilution to form gas concentration gradient, so can on a chip, while realize it is right under different condition
Cell carries out stimulation test, by once test just obtain it is multiple in the case of the stimulated result of cell.
Similar, the lower layer chip includes the liquid flow path being set up in parallel and the culture solution positioned at channel both sides
Entrance and waste liquid outlet, wherein culture solution entrance at least there are two, waste liquid outlet there is no limit, to form fluid gradient G.
The middle layer chip includes the array structure being made of cell culture well, the cell culture well and levels chip
In channel communicate, while cell culture well in load gel and cell, gel and the cell culture fluid in lower layer chip it is direct
Contact.
In the present invention, middle layer chip body cannot penetrate gas or liquid, therefore cell training is opened up above
Hole is supported, gel filled in hole, cell is cultivated in gel upper surface, and gel plays the role of interval and ditch aeration liquid at this time；In this way
Nutriment can be conveyed to cell by the solution of lower layer through gel infiltration, while the metabolite of cell being oozed by gel
Thoroughly to taking away in liquid, the stabilization of cellular environment is maintained.In addition, array mass detection may be implemented using array structure.
In the present invention, the channel direction in upper layer chip and lower layer chip is perpendicular or parallel.In the chip of upper layer, each row are
A kind of concentration conditions of gas, in lower layer chip, each row are a kind of concentration conditions of liquid, by taking 4*4 arrays as an example, altogether 4
Row, it is the stimulated experiment under identical conditions often to show 4 cells.It equally, can be as needed if being designed to 6*6 arrays
Change simultaneously corresponding fluid passage.At this time if liquid inlet is the liquid of two kinds of various concentrations, gas access be two kinds not
With the gas of concentration, gas-liquid channel is perpendicular, and the stimulation under 16 different conditions can be realized on chip；Gas-liquid channel can also
It is parallel, the stimulation under 4 different conditions can be realized on chip at this time.
The upper layer chip, middle layer chip and lower layer chip are combined by dimethyl silicone polymer layer and plastic layer.
Wherein, plastic layer is by polymethyl methacrylate, makrolon, polystyrene or polypropylene or polyethylene terephthalate
It is made.
The gel is the gel that disclosure satisfy that cell biological compatibility.Wherein, gel be agarose, collagen hydrogel,
Polylysine or chitosan.
It, can addition stimulating drug forms cell solution exposure dress in the cell culture fluid of lower layer chip again in the present invention
It sets, realizes the stimulation test to cell.Under conditions of analogue body inner cell growing environment, cell is stimulated, with observation
The existence metabolic condition of cell.Using solution exposing device, it can be achieved that screening to drug, to assess the drug effect of drug, this is right
It is significant in medicament research and development；Simultaneously can also analogue body inner cell by the pollutants such as flue gas stimulate, degree of impairment.For example,
Flue gas extract is dissolved in cell culture fluid, exposure stimulation cell can detect the variation of ROS, various transcription factors in cell
Situation.
Advantageous effect：Compared with prior art, remarkable advantage of the invention is：The fume exposure device includes upper, middle and lower
Three layers of chip structure；Wherein, upper layer chip can be passed through cigarette smoke, to stimulate cell；Middle layer chip is used for loading cells
And culture, lower layer chip lead to cell culture fluid, constantly to provide nutrient, and export cell metabolite in real time.In the present invention
Cell is located above gel, is not in direct contact with cell culture fluid, avoids culture solution and cause directly to impact to cell surface；Together
When culture solution again can pass through gel supply cell and keep cells survival ambient stable；Cell can be with flue gas on this basis
It is in direct contact, to obtain related biological information of the flue gas to cytositimulation.
Description of the drawings
Fig. 1 is the structural schematic diagram of fume exposure device of the present invention upper layer chip；
Fig. 2 is the structural schematic diagram of fume exposure device middle layer chip of the present invention；
Fig. 3 is the structural schematic diagram of fume exposure device lower layer chip of the present invention；
Fig. 4 is fume exposure device overall structure figure of the present invention；
Fig. 5 is the partial cutaway view of a certain row of fume exposure device of the present invention；
Fig. 6 is the experimental comparison group cell activity figure of fume exposure device of the present invention；
Fig. 7 is the fume exposure group cell activity figure of fume exposure device of the present invention.
In figure：The gas pipeline of the upper layers 1- chip；The gas accesses 2-；3- gas vents；The cell of 4- middle layer chips is trained
Support hole；The fluid passage of 5- lower layer chips；6- culture solution entrances；7- waste liquid outlets；8- cells；9- gels；10- living cells；11-
Apoptotic cell；12- non-viable non-apoptotic cells.
Specific implementation mode
Technical scheme of the present invention is described further below in conjunction with the accompanying drawings.
One, the manufacturing process of chip platform
1. upper layer chip：By uncured dimethyl silicone polymer (aggressiveness before PDMS：Curing agent=10:1), it is taped against upper layer
In chip template, to remove bubble, 70 DEG C of 45min that are heating and curing of warm table take off, are beaten in designated position vacuum suction 10min
Fig. 1 is seen in hole to get to upper layer chip.
2. middle layer chip：Using laser engraving machine, the poroid knot of 4*4 arrays is carved to the organic plastic plate of 0.5mm thickness
Structure, aperture 1mm, you can be used for loading cells, see Fig. 2.
3. lower layer chip：By uncured dimethyl silicone polymer (aggressiveness before PDMS：Curing agent=10:1), it is taped against lower layer
In chip template, to remove bubble, 70 DEG C of 45min that are heating and curing of warm table take off, are beaten in designated position vacuum suction 10min
Fig. 3 is seen in hole to get to lower layer chip.
4. intermediate layer cell culture hole and upper and lower layer chip channel are vertical corresponding, chip fits closely, and constitutes complete micro-
Flow control gel gas-liquid interface cell culture and fume exposure platform, are shown in Fig. 4-5.
Two, chip loading cells and culture
1. the low melting-point agarose gel solution for being prepared 2% using phosphate buffer solution (PBS), is placed in 70 DEG C of water-baths
Dissolving, concussion form homogeneous aqueous solution.
2. handling cell, 10 are obtained6The cell solution of cell/L.
3. 10min ultraviolet light sterilizations processing lower layer will be passed through and middle layer is taken out, is bonded, pipetted successively using liquid-transfering gun
2% gel, 0.7 μ L inject micropore, and chip is put into 4 DEG C of refrigerator 5min, gel solidification.
4. pipetting 0.7 μ L cell solutions injection micropore successively again using liquid-transfering gun.
5. syringe pump and 5mL syringes are used, with 5 μ L/min speed continuous pourings cell culture fluid 24 hours.
6. observing cell activity situation.
Three, chip cell fume exposure
1., will be on loading cells to chip by the above.
2. syringe pump and 5mL syringes are equally used, with 5 μ L/min speed continuous pouring cell culture fluids.
3. fume collection is connected to gas access in flue gas bag；Gas vent connects gas flowmeter and vacuum hair
Raw device；The cell fume exposure of certain flue gas flow rate can be realized, experiment exposes 20min with 8mL/min flow velocitys.
4. fluorescence probe dyestuff AO-EB incubated cells are used, Fluirescence observation, as shown in Figure 7.
5. Fig. 6 is experimental comparison group, i.e., stimulated without flue gas, the cell activity situation normally cultivated.
Situation is influenced on cell activity 6. thus calculating and obtaining flue gas, according to formula：Cell mortality=(apoptotic cell+
Non-viable non-apoptotic cell)/total number of cells.
7. Fig. 6 is experimental comparison group, cell mortality≤5%；Fig. 7 is fume exposure group, cell mortality >=50%；By
This can be seen that causes damage by flue gas to cell, which is effectively used for experimental study.
Gas-liquid interface is introduced micro-fluidic chip by the present invention, may be implemented to be exposed in gaseous environment human lung's cell
The good simulation of virtual condition, and on-line checking is carried out to important oxidative stress toxicity index, while having both micromation, automatically
Change and cheap feature.Therefore, the present invention is based on microfluidic chip technology, the exposure of research and development gas-liquid interface cell and corresponding cigarettes
The quick detection platform of gas oxidative stress toxicity index, the research for cigarette smoke Evaluation of Harmfulness and its toxic effect mechanism
It has great significance.
Claims (10)
1. a kind of micro-fluidic gel gas-liquid interface fume exposure device, it is characterised in that：Including providing the upper of flue gas access way
Layer chip provides the lower layer chip in cell culture solution flow channel and between levels chip for loading gel and position
In the middle layer chip of cell on gel；Wherein, the cell culture fluid in lower layer chip provides for the cell in middle layer chip
Living environment, the gel in middle layer chip become gas-liquid interface and its cell are made to be in direct contact with flue gas.
2. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：The upper layer chip
Gas access way including being set up in parallel and the gas access positioned at channel both sides and gas vent, wherein gas access
There are two at least, to form gas concentration gradient.
3. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：The lower layer chip
Including the liquid flow path being set up in parallel and positioned at the culture solution entrance and waste liquid outlet of channel both sides, wherein culture solution
Entrance at least there are two, to form fluid gradient G.
4. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：The middle layer core
Piece includes the array structure being made of cell culture well, which communicates with the channel in levels chip, while thin
Gel is loaded in born of the same parents' culture hole and cell, gel are in direct contact with the cell culture fluid in lower layer chip.
5. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：The upper layer chip
It is perpendicular or parallel with the channel direction in lower layer chip.
6. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：The upper layer core
Piece, middle layer chip and lower layer chip are combined by dimethyl silicone polymer layer and plastic layer.
7. micro-fluidic gel gas-liquid interface fume exposure device according to claim 6, it is characterised in that：The plastic layer by
Polymethyl methacrylate, makrolon, polystyrene or polypropylene or polyethylene terephthalate are made.
8. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：The gel is energy
Enough meet the gel of cell biological compatibility.
9. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：The gel is fine jade
Lipolysaccharide, collagen hydrogel, polylysine or chitosan.
10. micro-fluidic gel gas-liquid interface fume exposure device according to claim 1, it is characterised in that：Lower layer's core
Stimulating drug is added in the cell culture fluid of piece forms cell solution exposing device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610826876.0A CN106399076B (en) | 2016-09-14 | 2016-09-14 | Micro-fluidic gel gas-liquid interface fume exposure device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610826876.0A CN106399076B (en) | 2016-09-14 | 2016-09-14 | Micro-fluidic gel gas-liquid interface fume exposure device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106399076A CN106399076A (en) | 2017-02-15 |
| CN106399076B true CN106399076B (en) | 2018-10-02 |
Family
ID=57997349
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610826876.0A Active CN106399076B (en) | 2016-09-14 | 2016-09-14 | Micro-fluidic gel gas-liquid interface fume exposure device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106399076B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112300929B (en) * | 2019-07-31 | 2022-12-02 | 上海新微技术研发中心有限公司 | Microfluidic experiment plate and double-sided cell culture method |
| CN111019828B (en) * | 2019-12-26 | 2021-12-21 | 武汉大学 | Open type high-flux microfluidic oocyte dynamic three-dimensional culture chip and application thereof |
| CN111019827A (en) * | 2019-12-26 | 2020-04-17 | 武汉大学 | Hydrogel oocyte in-vitro three-dimensional culture micro-fluidic chip based on different cross-linking degrees and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| DE19811735C2 (en) * | 1998-03-18 | 2000-10-19 | Reinhard K Teichmann | Procedure for determining the influence of an influence on a respiratory system |
| JP2004222533A (en) * | 2003-01-20 | 2004-08-12 | Japan Tobacco Inc | Smoke exposure testing module, and smoke exposure testing method using the module |
| CN100458442C (en) * | 2002-05-24 | 2009-02-04 | 英美烟草(投资)有限公司 | An exposure device |
| WO2013064466A1 (en) * | 2011-10-31 | 2013-05-10 | British American Tobacco (Investments) Limited | Fluid distribution in a sample exposure device |
| CN203754721U (en) * | 2013-11-15 | 2014-08-06 | 北京慧荣和科技有限公司 | Cell gas exposure system |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201092574Y (en) * | 2007-09-29 | 2008-07-30 | 中国烟草总公司郑州烟草研究院 | Mainstream smoke exposing device for cigarette external toxicology |
| CN201842846U (en) * | 2010-10-28 | 2011-05-25 | 天津开发区合普工贸有限公司 | Control device of cell exposure gas with controllable concentration |
| CN102517204B (en) * | 2011-12-21 | 2013-09-11 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | Cell gas poisoning apparatus |
| CN102676382A (en) * | 2012-05-29 | 2012-09-19 | 云南烟草科学研究院 | Cell gas-liquid interface contact type cigarette whole smoke exposure device |
| CN103146570A (en) * | 2013-03-28 | 2013-06-12 | 云南烟草科学研究院 | Direct exposure system of germ/cell flue gas |
-
2016
- 2016-09-14 CN CN201610826876.0A patent/CN106399076B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19526533A1 (en) * | 1995-07-20 | 1997-01-23 | Fraunhofer Ges Forschung | Nutritional medium delivered to cell culture through permeable membrane |
| DE19811735C2 (en) * | 1998-03-18 | 2000-10-19 | Reinhard K Teichmann | Procedure for determining the influence of an influence on a respiratory system |
| CN100458442C (en) * | 2002-05-24 | 2009-02-04 | 英美烟草(投资)有限公司 | An exposure device |
| JP2004222533A (en) * | 2003-01-20 | 2004-08-12 | Japan Tobacco Inc | Smoke exposure testing module, and smoke exposure testing method using the module |
| WO2013064466A1 (en) * | 2011-10-31 | 2013-05-10 | British American Tobacco (Investments) Limited | Fluid distribution in a sample exposure device |
| CN203754721U (en) * | 2013-11-15 | 2014-08-06 | 北京慧荣和科技有限公司 | Cell gas exposure system |
Non-Patent Citations (6)
| Title |
|---|
| Comet assay and air-liquid interface exposure system: a new combination to evaluate genotoxic effects of cigarette whole smoke in human lung cell lines;Weber S 等;《Toxicology in Vitro》;20130930;第27卷(第6期);全文 * |
| Continual Exposure to Cigarette Smoke Extracts Induces Tumor-Like Transformation of Human Nontumor Bronchial Epithelial Cells in a Microfluidic Chip;Encheng Li 等;《Journal of Thoracic Oncology》;20140831;第9卷(第8期);全文 * |
| Macrophages promote benzopyrene-induced tumor transformation of human bronchial epithelial cells by activation of NF-κB and STAT3 signaling in a bionic airway chip culture and in animal models;Encheng Li 等;《Oncotarget》;20150312;第6卷(第11期);第8910页右栏第2段，图1及其图注 * |
| 基于微流控芯片技术COPD向肺鳞癌恶性转化的机制研究;李恩成;《中国博士学位论文全文数据库-医药卫生科技辑》;20150131(第1期);第54页第6节，第57页第1节，图16 * |
| 微纳尺度管道的构建、表征及应用;徐碧漪;《中国博士学位论文全文数据库-信息科技辑》;中国学术期刊（光盘版）电子杂志社有限公司;20140531(第5期);全文 * |
| 芯片上稳定气液双重梯度的形成及其在无剪切力的悬浮细胞毒性试验中的应用;徐碧漪，胡善文，徐静娟，陈洪渊;《2012年全国微纳尺度生物分离分析学术会议、第七届全国微全分析系统学术会议暨第三届国际微流控分析(西湖)学术论坛论文集》;20130918;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106399076A (en) | 2017-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Esch et al. | The role of body-on-a-chip devices in drug and toxicity studies | |
| US20210079330A1 (en) | Dynamic multi organ plate | |
| CN106399076B (en) | Micro-fluidic gel gas-liquid interface fume exposure device | |
| US20150247112A1 (en) | 3D Tissue Culture Devices and Systems | |
| US20210341462A1 (en) | Artificial human pulmonary airway and methods of preparation | |
| CN103476920A (en) | Apparatuses for and methods of processing cells and related structures | |
| WO2013086486A1 (en) | Integrated human organ-on-chip microphysiological systems | |
| Ponmozhi et al. | Development of skin-on-a-chip platforms for different utilizations: Factors to be considered | |
| Müller et al. | Measurement of respiration and acidification rates of mammalian cells in thermoplastic microfluidic devices | |
| US20200392440A1 (en) | Multigel tumor-on-a-chip system | |
| Bennet et al. | Airway-on-a-chip: designs and applications for lung repair and disease | |
| Grist et al. | Designing a microfluidic device with integrated ratiometric oxygen sensors for the long-term control and monitoring of chronic and cyclic hypoxia | |
| CN101268365A (en) | Chemosensitivity tester | |
| JP2018515146A (en) | Microfluidic device for in vitro 3D cell culture experiments | |
| Haque et al. | Organ-chip models: opportunities for precision medicine in pancreatic cancer | |
| CN103981085A (en) | Self-set concentration gradient drug screening organ chip and preparation method thereof | |
| Liu et al. | Recent advances of organ-on-a-chip in cancer modeling research | |
| Jandova et al. | Deuterium oxide (D2O) induces early stress response gene expression and impairs growth and metastasis of experimental malignant melanoma | |
| Herreros et al. | Alternative brain slice-on-a-chip for organotypic culture and effective fluorescence injection testing | |
| Sylman et al. | An Inquiry-based investigation of controlled-release drug delivery from hydrogels: an experiment for high school chemistry and biology | |
| Tan et al. | Exploratory evaluation of EGFR-targeted anti-tumor drugs for lung cancer based on lung-on-a-chip | |
| Nguyen et al. | 3d in vitro platform for cell and explant culture in liquid-like solids | |
| Naumann et al. | Combined systemic drug treatment with proton therapy: investigations on patient-derived organoids | |
| Dance | Building benchtop human models | |
| CN108138135A (en) | Cell culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |