CN211689079U - Air-lift type amino acid fermentation tank - Google Patents
Air-lift type amino acid fermentation tank Download PDFInfo
- Publication number
- CN211689079U CN211689079U CN201922236548.7U CN201922236548U CN211689079U CN 211689079 U CN211689079 U CN 211689079U CN 201922236548 U CN201922236548 U CN 201922236548U CN 211689079 U CN211689079 U CN 211689079U
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- Prior art keywords
- fermentation
- amino acid
- tank body
- cooling
- defoaming
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- 238000000855 fermentation Methods 0.000 title claims abstract description 76
- 230000004151 fermentation Effects 0.000 title claims abstract description 76
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 25
- 238000001816 cooling Methods 0.000 claims abstract description 35
- 239000000463 material Substances 0.000 claims description 15
- 239000000498 cooling water Substances 0.000 claims description 12
- 238000005070 sampling Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 8
- 230000001502 supplementing effect Effects 0.000 claims description 8
- 238000007599 discharging Methods 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 229910000619 316 stainless steel Inorganic materials 0.000 claims description 3
- 239000005388 borosilicate glass Substances 0.000 claims description 3
- 230000009286 beneficial effect Effects 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 238000002425 crystallisation Methods 0.000 abstract description 7
- 230000008025 crystallization Effects 0.000 abstract description 7
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 5
- 239000001301 oxygen Substances 0.000 abstract description 5
- 229910052760 oxygen Inorganic materials 0.000 abstract description 5
- 238000010008 shearing Methods 0.000 abstract description 5
- 239000002518 antifoaming agent Substances 0.000 abstract description 3
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 239000006260 foam Substances 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 2
- 239000007789 gas Substances 0.000 description 14
- 239000000047 product Substances 0.000 description 8
- 239000013078 crystal Substances 0.000 description 5
- 239000006052 feed supplement Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000010907 mechanical stirring Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000002411 adverse Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
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Abstract
The utility model provides an air-lift formula amino acid fermentation cylinder utilizes aseptic air to drive the zymotic fluid circulation, utilizes pH electrode, dissolved oxygen electrode to detect fermentation process, utilizes the defoaming oar to combine the foam state in the defoaming agent control fermentation cylinder, utilizes two sections cooling to form the temperature difference about the fermentation cylinder, and zymotic fluid circulation accelerates, promotes heat and mass transfer, and the shearing force is less among the fermentation process, helps the fermentation product crystallization, forms bigger result crystallization, is favorable to the collection separation of crystallization product.
Description
Technical Field
The utility model relates to a bioengineering equipment, especially an air-lift formula amino acid fermentation cylinder.
Background
Traditional amino acid fermentation equipment is mainly mechanical stirring ventilation formula fermentation cylinder, in order to satisfy dissolved oxygen demand in amino acid fermentation process, improves stirring rotational speed usually, but high stirring rotational speed causes the shearing force too big, produces adverse effect to the lower amino acid crystallization process of solubility, causes the fine crushing of formation crystal, causes the difficulty for the separation extraction of low reaches amino acid product, increases product manufacturing cost.
The airlift fermentation tank has no mechanical stirring, and the fermentation liquid is driven by sterile air to form circulation in the fermentation tank, so that the airlift fermentation tank has the advantages of small shearing force, low energy consumption, high air utilization efficiency and the like. However, the traditional airlift fermentation tank has no devices such as pH, dissolved oxygen, defoaming paddle and the like, and causes difficulties in analysis and control of the amino acid fermentation process, amplification of a reactor and the like.
SUMMERY OF THE UTILITY MODEL
The technical problem to be solved by the utility model is to provide an air-lift amino acid fermentation tank.
In order to solve the technical problem, the technical scheme of the utility model is that:
an airlift amino acid fermentation tank comprises an end cover and a tank body, wherein the end cover and the tank body are connected by flanges,
the end cover comprises a flange plate (3), a defoaming motor (1), a defoaming paddle (15), a material supplementing hole (2), a material inoculating hole (16) and a tail gas pipe (17), wherein the defoaming motor (1) is connected with the defoaming paddle (15) through a stirring shaft, the defoaming motor (1) is positioned at the central position of the end cover, the stirring shaft is connected with the flange plate (3) through a shaft seal, the defoaming motor (1) and the defoaming paddle (15) are respectively arranged on the upper side and the lower side of the flange plate (1), and the material supplementing hole (2), the material inoculating hole (16), the tail gas pipe (17) and fixing screws (18) are respectively distributed on the flange plate (3);
the middle part of the tank body is a cylinder, the bottom part of the tank body is a cone, the middle lower part of the cylinder and the outer side of the bottom part of the cone are sequentially wrapped with a middle cooling jacket (4) and a bottom cooling jacket (7), one side of the middle part of the tank body is provided with a sight hole (14), the lower part of the sight hole is provided with a sampling pipe (5), the sampling pipe (5) is communicated with a stop valve inside and outside the fermentation tank, the fermentation tank is used for sampling in the fermentation process, three sockets are arranged on one side of the middle of the tank body, a thermometer sleeve (10), a first sensor (11) and a second sensor (12) are respectively installed on the sockets, a guide cylinder (13) used for guiding the flow of fermentation liquor is arranged in the tank body, a gas distributor (9) is arranged on the lower portion of the guide cylinder (13) of the tank body, and a vent pipe (6) penetrates through the middle-upper portion of the curved surface of the bottom of the cone of the tank body from the outside of the fermentation tank and is communicated with the gas distributor (9) and; the bottom of the tank body is provided with a discharge hole (8) for discharging wastes in the fermentation tank.
The socket arranged in the middle of the tank body can be provided with a temperature electrode, a dissolved oxygen electrode, a pH electrode, an online turbidity sensor, a glucose concentration online sensor and the like when necessary.
Preferably, the number of the material supplementing openings (2) is three to better meet the requirement of the fermentation process.
Preferably, in order to meet the requirements of structural strength and air tightness of flange connection between the end cover of the fermentation tank and the tank body, the flange plate (3) is symmetrically provided with 10 fixing screws (18).
Preferably, the material of the middle cooling jacket (4) and the material of the bottom cooling jacket (7) of the airlift amino acid fermentation tank are both 316 stainless steel.
Preferably, in the air-lift amino acid fermentation tank, the guide cylinder (13) is spirally raised around the outer side of the inner wall (13-2) by a semicircular cooling coil (13-4) and welded together, and a cooling pipe cavity (13-5) is formed between the cooling coil and the inner wall for cooling water to flow inside for heat exchange and cooling of fermentation liquor; one side of the upper part of the guide shell cooling pipe and the other side of the symmetrical lower part are respectively provided with a cooling water inlet (13-3) and a cooling water outlet (13-1).
Preferably, the conical angle of the bottom of the cone is 60-70 degrees, so that the collection of the crystal product formed by fermentation is facilitated.
Preferably, in the airlift amino acid fermentation tank, the sight hole (14) is made of transparent borosilicate glass, so that the condition in the fermentation tank can be observed conveniently.
Preferably, in the airlift amino acid fermentation tank, the gas distributor (9) is a circular tube type gas distributor, and the circular tube (8-1) is provided with spray holes (8-2) with upward openings, so that sterile air is dispersed into small bubbles as much as possible, and the gas-liquid contact area is increased.
Has the advantages that:
according to the air-lift type amino acid fermentation tank, sterile air is used for driving the fermentation liquor to circulate, the fermentation process is detected by using the pH electrode and the dissolved oxygen electrode, the defoaming paddle is combined with the defoaming agent to control the foam state in the fermentation tank, the temperature difference between the upper part and the lower part of the fermentation tank is formed by using two-stage cooling, the circulation of the fermentation liquor is accelerated, the heat transfer and mass transfer are promoted, the shearing force in the fermentation process is small, the crystallization of a fermentation product is facilitated, the larger product crystallization is formed, and the collection.
Drawings
FIG. 1 is a front view of the structure of the present invention;
FIG. 2 is a left side view of the structure of the present invention;
FIG. 3 is a schematic top view of the present invention;
FIG. 4 is a partial cross-sectional view of a draft tube;
FIG. 5 is a schematic view of an end cap configuration;
FIG. 6 is a cross-sectional view of an air distributor;
fig. 7 is a schematic diagram of the operation of the apparatus.
In the figure, 1, a defoaming motor, 2, a material supplementing port, 3, a flange plate, 4, a middle cooling jacket, 5, a sampling tube, 6, a vent pipe, 7, a bottom cooling jacket, 8, a material discharging port, 9, a gas distributor, 10, a thermometer sleeve, 11, a first sensor, 12, a second sensor, 13, a guide cylinder, 14, a sight hole, 15, a defoaming paddle, 16, a seed receiving port, 17, a tail gas pipe, 18, a fixing screw, 8-1-ring pipes, 8-2-spray holes, 13-1-cooling water outlet, 13-2-inner walls, 13-3-cooling water inlet, 13-4-cooling coil pipes and 13-5-cooling pipe cavities are arranged.
Detailed Description
Example 1
As shown in figures 1-6, the airlift amino acid fermentation tank comprises an end cover and a tank body, wherein the end cover and the tank body are connected by flanges,
the end cover comprises a flange plate 3, a defoaming motor 1, a defoaming paddle 15, a feed supplement port 2, an inoculation port 16 and a tail gas pipe 17, wherein the defoaming motor 1 is connected with the defoaming paddle 15 through a stirring shaft, the defoaming motor 1 is positioned at the central position of the end cover, the stirring shaft is connected with the flange plate 3 through a shaft seal, the defoaming motor 1 and the defoaming paddle 15 are respectively arranged at the upper side and the lower side of the flange plate 1, the feed supplement port 2, the inoculation port 16, the tail gas pipe 17 and ten fixing screws 18 are symmetrically distributed on the flange plate 3, and the number of the feed supplement ports is three in order to meet the requirement of a;
the middle part of the tank body is a cylinder, the bottom of the tank body is a cone, the cone angle of the bottom of the cone is 60-70 degrees, the collection of a crystallization product formed by fermentation is facilitated, the middle lower part of the cylinder and the outer side of the bottom of the cone are sequentially wrapped by a middle cooling jacket 4 and a bottom cooling jacket 7, the middle cooling jacket 4 and the bottom cooling jacket 7 are made of 316 stainless steel, one side of the middle part of the tank body is provided with a sight hole 14, the sight hole 14 is made of transparent borosilicate glass and facilitates observation of the condition in the fermentation tank, the lower part of the sight hole is provided with a sampling tube 5, the sampling tube 5 is communicated with a stop valve inside and an external stop valve and is used for sampling in the fermentation process, one side of the middle part of the tank body is provided with three sockets, and is respectively provided with a thermometer sleeve 10, a first, pH electrode, on-line turbidity sensor, glucose concentration on-line sensor, etc. A guide cylinder 13 for guiding the flowing of the fermentation liquor is arranged in the tank body, the guide cylinder 13 is spirally raised around the outer side of the inner wall 13-2 by a semicircular cooling coil 13-4 for assisting in controlling the temperature of the fermentation liquor and is welded together, a cooling pipe cavity 13-5 is formed between the cooling coil and the inner wall, and cooling water flows inside the cooling pipe cavity for heat exchange and cooling of the fermentation liquor; one side of the upper part and the other side of the symmetrical lower part of the cooling pipe of the guide shell are respectively provided with a cooling water inlet 13-3 and a cooling water outlet 13-1; the lower part of the tank body draft tube 13 is provided with a gas distributor 9, the gas distributor 9 is a circular tube type gas distributor, the circular tube 8-1 is provided with spray holes 8-2 with upward openings, so that sterile air is dispersed into small bubbles as much as possible, the gas-liquid contact area is increased, and the vent pipe 6 penetrates through the middle upper part of the curved surface of the bottom of the cone of the tank body from the outside of the fermentation tank and is communicated with the gas distributor 9 for introducing the sterile air into the fermentation tank; the bottom of the tank body is provided with a discharge hole 8 for discharging wastes in the fermentation tank.
As shown in FIG. 7, sterile air enters the fermentation tank through the air inlet pipe 6 and the air distributor 9 to drive the fermentation liquid to circulate, and meanwhile, the temperature of the conical bottom fermentation liquid is controlled by the bottom cooling jacket 7 and the middle jacket 4 to be slightly higher than the temperature of the middle fermentation liquid of the fermentation tank, so that the formed temperature gradient is favorable for the bottom fermentation liquid to move upwards, and the circulation of the fermentation liquid is accelerated.
When producing a large amount of bubbles in the fermentation process, can follow eyehole 14 discovery, open defoaming motor 1, carry out the defoaming through defoaming oar 15, also can flow from feed supplement mouth 2 and add certain defoaming agent when necessary. In addition, parameters such as cooling water flow rate of the jacket, material supplementing rate, ventilation quantity and the like are adjusted in time according to the temperature sensor 10 and other sensors (electrodes), so that the fermentation process is accurately controlled.
The product crystals in the fermentation tank form larger crystal particles due to smaller shearing force, and are deposited at the bottom of the fermentation tank under the action of gravity, and meanwhile, the product crystals can be discharged from a bottom discharge port 8 due to the fact that the pressure in the fermentation tank is higher than atmospheric pressure, and the utilization rate of the fermentation tank is improved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. The utility model provides an air-lift formula amino acid fermentation cylinder, includes the end cover and jar body, the end cover adopts flange joint, its characterized in that with jar body: wherein the content of the first and second substances,
the end cover comprises a flange plate (3), a defoaming motor (1), a defoaming paddle (15), a material supplementing hole (2), a material inoculating hole (16) and a tail gas pipe (17), wherein the defoaming motor (1) is connected with the defoaming paddle (15) through a stirring shaft, the defoaming motor (1) is positioned at the central position of the end cover, the stirring shaft is connected with the flange plate (3) through a shaft seal, the defoaming motor (1) and the defoaming paddle (15) are respectively arranged on the upper side and the lower side of the flange plate (3), and the material supplementing hole (2), the material inoculating hole (16), the tail gas pipe (17) and fixing screws (18) are respectively distributed on the flange plate (3);
the middle part of the tank body is a cylinder, the bottom part of the tank body is a cone, the middle lower part of the cylinder and the outer side of the bottom part of the cone are sequentially wrapped with a middle cooling jacket (4) and a bottom cooling jacket (7), one side of the middle part of the tank body is provided with a sight hole (14), the lower part of the sight hole is provided with a sampling pipe (5), the sampling pipe (5) is communicated with a stop valve inside and outside the fermentation tank, the fermentation tank is used for sampling in the fermentation process, three sockets are arranged on one side of the middle of the tank body, a thermometer sleeve (10), a first sensor (11) and a second sensor (12) are respectively installed on the sockets, a guide cylinder (13) used for guiding the flow of fermentation liquor is arranged in the tank body, a gas distributor (9) is arranged on the lower portion of the guide cylinder (13) of the tank body, and a vent pipe (6) penetrates through the middle-upper portion of the curved surface of the bottom of the cone of the tank body from the outside of the fermentation tank and is communicated with the gas distributor (9) and; the bottom of the tank body is provided with a discharge hole (8) for discharging wastes in the fermentation tank.
2. The airlift amino acid fermenter of claim 1, wherein: the number of the feed supplementing openings (2) is three.
3. The airlift amino acid fermenter of claim 1, wherein: the flange plate (3) is symmetrically provided with 10 fixing screws (18).
4. The airlift amino acid fermenter of claim 1, wherein: the middle cooling jacket (4) and the bottom cooling jacket (7) are both made of 316 stainless steel.
5. The airlift amino acid fermenter of claim 1, wherein: the guide cylinder (13) is spirally raised around the outer side of the inner wall (13-2) by a semicircular cooling coil (13-4) and is welded together, and a cooling pipe cavity (13-5) is formed between the cooling coil and the inner wall for cooling water to flow inside for heat exchange and cooling of fermentation liquor; one side of the upper part of the guide shell cooling pipe and the other side of the symmetrical lower part are respectively provided with a cooling water inlet (13-3) and a cooling water outlet (13-1).
6. The airlift amino acid fermenter of claim 1, wherein: the cone angle of the bottom of the cone is between 60 and 70 degrees, which is beneficial to the collection of the crystalline product formed by fermentation.
7. The airlift amino acid fermenter of claim 1, wherein: the material of sight hole (14) is transparent borosilicate glass, is convenient for observe the interior condition of fermentation cylinder.
8. The airlift amino acid fermenter of claim 1, wherein: the gas distributor (9) is a circular tube type gas distributor, and the circular tube (8-1) is provided with spray holes (8-2) with upward openings, so that sterile air is dispersed into small bubbles as much as possible, and the gas-liquid contact area is increased.
Priority Applications (1)
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CN201922236548.7U CN211689079U (en) | 2019-12-13 | 2019-12-13 | Air-lift type amino acid fermentation tank |
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CN201922236548.7U CN211689079U (en) | 2019-12-13 | 2019-12-13 | Air-lift type amino acid fermentation tank |
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Cited By (7)
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CN112625915A (en) * | 2020-11-30 | 2021-04-09 | 江苏神华药业有限公司 | Method for fermenting coriolus versicolor by using airlift fermentation tank fed batch |
CN112625914A (en) * | 2020-11-30 | 2021-04-09 | 江苏神华药业有限公司 | Method for repeatedly fermenting lucid ganoderma in batches by using airlift fermentation tank |
CN112940945A (en) * | 2020-12-18 | 2021-06-11 | 江苏神华药业有限公司 | Method for fermenting hirsutella sinensis |
CN113273607A (en) * | 2021-06-04 | 2021-08-20 | 童军 | Dairy product fermentation system and fermentation method |
CN114085753A (en) * | 2021-11-22 | 2022-02-25 | 石家庄吉瑞节能技术有限公司 | Forced circulation flow energy-saving fermentation tank |
CN114195252A (en) * | 2021-11-26 | 2022-03-18 | 大唐东北电力试验研究院有限公司 | Air-lift reactor for treating and refining soybean oil wastewater by using fermentation type hyphomycete |
CN115558577A (en) * | 2022-10-13 | 2023-01-03 | 齐鲁工业大学 | Fermentation desublimation crystallization device of tetramethylpyrazine |
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2019
- 2019-12-13 CN CN201922236548.7U patent/CN211689079U/en not_active Expired - Fee Related
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN112625915A (en) * | 2020-11-30 | 2021-04-09 | 江苏神华药业有限公司 | Method for fermenting coriolus versicolor by using airlift fermentation tank fed batch |
CN112625914A (en) * | 2020-11-30 | 2021-04-09 | 江苏神华药业有限公司 | Method for repeatedly fermenting lucid ganoderma in batches by using airlift fermentation tank |
CN112625914B (en) * | 2020-11-30 | 2023-04-21 | 江苏神华药业有限公司 | Method for repeatedly fermenting ganoderma lucidum in batches by using airlift fermentation tank |
CN112940945A (en) * | 2020-12-18 | 2021-06-11 | 江苏神华药业有限公司 | Method for fermenting hirsutella sinensis |
CN113273607A (en) * | 2021-06-04 | 2021-08-20 | 童军 | Dairy product fermentation system and fermentation method |
CN114085753A (en) * | 2021-11-22 | 2022-02-25 | 石家庄吉瑞节能技术有限公司 | Forced circulation flow energy-saving fermentation tank |
CN114195252A (en) * | 2021-11-26 | 2022-03-18 | 大唐东北电力试验研究院有限公司 | Air-lift reactor for treating and refining soybean oil wastewater by using fermentation type hyphomycete |
CN115558577A (en) * | 2022-10-13 | 2023-01-03 | 齐鲁工业大学 | Fermentation desublimation crystallization device of tetramethylpyrazine |
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Granted publication date: 20201016 |