CN211620497U

CN211620497U - Kit heating oscillation device

Info

Publication number: CN211620497U
Application number: CN202020034859.5U
Authority: CN
Inventors: 王凤羽; 马冉冉; 张辉
Original assignee: Zhengzhou Huazhiyuan Medical Laboratory Co ltd
Current assignee: Zhengzhou Huazhiyuan Medical Laboratory Co ltd
Priority date: 2020-01-08
Filing date: 2020-01-08
Publication date: 2020-10-02
Anticipated expiration: 2030-01-08

Abstract

The utility model provides a kit heating oscillation device, relate to the mixed machine field that has the shake mechanism, which comprises a housin, the fixed heating pipe that is equipped with on the inside wall of casing, it is equipped with the annular still to fix on the inside wall of casing, the embedded rubber pad that is equipped with of annular, be equipped with the water bath bucket in the casing, the water bath bucket is placed on the rubber pad, the spout group has been seted up on the inside wall of water bath bucket, sliding connection has same bracing piece between the spout group, the bottom of water bath bucket is connected with the vibration dish, the casing bottom is rotated and is connected with the pivot, the pivot upper end stretches into in the casing and fixedly connected with rack, the rack top can be dismantled and be connected. The utility model discloses utilize interference between the arch to make the innovation point of vibration, the vibration that the kit received is more even than manual oscillation, more mitigatees than the oscillator vibration, and the vibrational force that every sample received all is unanimous, reaches and lets more steady, abundant purpose of sample cell lysis process.

Description

Kit heating oscillation device

Technical Field

The utility model belongs to the technical field of the mixer with shake mechanism and specifically relates to a kit heating oscillation device.

Background

In the process of gene extraction and purification, SDS is added into a test tube, and the SDS is sodium dodecyl sulfate, so that protein can be denatured and separated from DNA in the process of gene extraction, and after the SDS is added, the cell is shaken to disintegrate and crack, and the conventional shaking process is mostly manual shaking and incomplete in cracking, but the use of a multi-frequency oscillator can cause the breakage of organelles due to overhigh vibration intensity. In addition, the number of single oscillation of manual oscillation is limited, and most oscillators adopt a rotary centrifugation mode, so that the rotary strength of each unit in each test tube or kit is different, and the cracking condition is different.

SUMMERY OF THE UTILITY MODEL

Aiming at the situation, in order to overcome the defects of the prior art, the utility model aims at providing a kit, which can evenly and mildly carry out water bath constant temperature oscillation on the sample in the kit, so as to lead the cells to be fully cracked.

The heating and oscillating device for the kit comprises a shell and is characterized in that a heating pipe is fixedly arranged on the inner side wall of the shell, an annular groove is fixedly and convexly arranged on the inner side wall of the shell, a rubber pad is embedded in the annular groove, a water bath barrel with an open upper end is arranged in the shell, an extending part is convexly arranged on the upper edge of the water bath barrel and is placed on the rubber pad, a plurality of sliding groove groups are arranged on the inner side wall of the water bath barrel, each sliding groove group comprises two corresponding sliding grooves, the same supporting rod is slidably connected between the two corresponding sliding grooves, all the supporting rods are parallel to each other, a plurality of holes are also arranged on the side wall of the water bath barrel, the bottom of the water bath barrel is detachably connected with an upper vibrating disc, a plurality of bulges are arranged on the lower surface of the upper vibrating disc, the bottom of the shell is rotatably connected with a rotating shaft, the upper, the rack top can be dismantled and be connected with down the vibration dish, and the upper surface of vibration dish is protruding to be equipped with a plurality of archs down, and protruding equipartition sets up, vibration dish and last vibration dish interfere the setting down.

Preferably, the shell external sealing connection has the heat preservation, is the vacuum region between heat preservation and the shell, the intercommunication has the inlet tube on the lateral wall of shell, and the inlet tube stretches out the heat preservation and with heat preservation sealing connection, the intercommunication has the outlet pipe on the bottom surface of shell.

Preferably, an upper cover is arranged above the shell, an annular convex edge is fixedly arranged on the upper surface of the shell upwards, the outer diameter of the convex edge is consistent with the outer diameter of the shell, the inner diameter of the convex edge is larger than the inner diameter of the shell, the diameter of the upper cover is consistent with the inner diameter of the convex edge, and a sealing ring embedded on the shell is arranged on a contact part of the upper cover and the shell.

Preferably, the convex edge is rotatably connected with a pressure head, and after the upper cover is covered on the shell, the edge of the upper cover is flush with the convex edge.

Preferably, the protrusions are hemispherical structures.

The utility model has the advantages that: compare prior art has the innovation point that makes the vibration with the interference between the arch, the arch on the vibration dish is constantly lifted on the lower vibration dish, and whole water bath bucket bears multi-direction complex vibration, and the vibration that the kit received is more even than manual oscillation, more mitigatees than the oscillator vibration, and the vibrational force that each sample received all is unanimous, combines homothermal water bath environment, reaches and lets more steady, abundant purpose of sample cell lysis process.

Drawings

FIG. 1 is a sectional view of the present invention;

FIG. 2 is a schematic view of the present water tub;

FIG. 3 is a schematic view of the outer structure of the present invention;

fig. 4 is an enlarged view of I in fig. 2.

Detailed Description

The following describes the embodiments of the present invention in further detail with reference to the accompanying drawings.

As shown in fig. 1-4, a kit heating and oscillating device comprises a casing 1, and is characterized in that a heating pipe 2 is fixedly arranged on the inner side wall of the casing 1, after water is injected into the casing 1, the heating pipe 2 starts heating water to make the temperature of the water bath reach the temperature suitable for storing samples, an annular groove 101 is fixedly and convexly arranged on the inner side wall of the casing 1, a rubber gasket 102 is embedded in the annular groove 101, a water bath barrel 3 with an open upper end is arranged in the casing 1, an extending part 31 is convexly arranged on the upper edge of the water bath barrel 3, the extending part 31 is arranged on the rubber gasket 102, the water bath barrel 3 is supported by the rubber gasket 102 and the annular groove 101, because the rubber gasket 102 is an elastic part, a space capable of moving exists when the water bath barrel 3 is subjected to an external force, the floating connection effect between the water bath barrel 3 and the casing 1 is realized, a plurality of sliding grooves 32 groups are arranged on, the chute 32 group comprises two corresponding chutes 32, the same support rod 33 is connected between the two corresponding chutes 32 in a sliding manner, all the support rods 33 are parallel to each other, the types of the reagent kit 14 are multiple and various, some reagent kits 14 are not provided with covers and are directly placed at the bottom of the water bath barrel 3, the samples are submerged to cause the water inlet failure of the samples, in order to improve the universality of the water bath barrel 3, the length of each chute 32 is set to be shorter than the height of the water bath barrel 3 to prevent the reagent kit 14 from being submerged due to too low position, and different from the plane support of a common oscillator, the reagent kit 14 is supported by the support rods 33 which are parallel to each other, even if water enters the reagent kit 14, the water can quickly fall back into the water bath barrel 3, all the reagent kits 14 can be placed into the water bath barrel 3, test tubes with longer bottoms can also extend out from the two support rods 33, and the side wall of the water bath, the water enters the water bath barrel 3 from the hole 34 to submerge the support frame and surround the middle lower part of the reagent box 14, the bottom of the water bath barrel 3 is detachably connected with an upper vibration disk 9, a plurality of bulges are arranged on the lower surface of the upper vibration disk 9 in a protruding manner, the bottom of the shell 1 is rotatably connected with a rotating shaft 11, the upper end of the rotating shaft 11 extends into the shell 1 and is fixedly connected with a horizontal placing frame 12, a lower vibration disk 13 is detachably connected above the placing frame 12, the lower end of the rotating shaft 11 is connected with a speed reducer or a motor, the rotating shaft 11 rotates at a low speed, meanwhile, the lower vibration disk 13 on the placing frame 12 also synchronously rotates, the motor can be connected with a time relay, the timing function of vibration time is not limited to be realized by a single chip microcomputer or the time relay, the upper surface of the lower vibration disk 13 is convexly provided with a plurality of bulges, the bulges are uniformly distributed, the lower vibration disk 13 and the upper vibration disk, the lower vibration disk 13 continuously jacks up the protrusions on the upper vibration disk 9 when rotating to cause the vibration of the water bath barrel 3, although the rotating shaft 11 rotates at a low speed, because the number of the protrusions is large, the contact and separation processes between every two protrusions cause one vibration, the vibration frequency transmitted to the water bath barrel 3 is very high, namely the protrusions play a role in raising the frequency, the supporting rod 33 is in sliding connection with the sliding groove 32, the amplitude transmitted from the protrusions and the side wall is amplified by the supporting rod 33, and the vibration effect of the reagent kit 14 per se is very obvious; the reagent vessel 14 itself is not subjected to significant centrifugal forces and the projections are evenly distributed, meaning that the vibrations imparted to each sample tube in the reagent vessel 14 are uniform.

The interference degree between the protrusions on the upper vibrating disk 9 and the protrusions on the lower vibrating disk 13 determines the vibration amplitude of the water bath barrel 3, when the amplitude is adjusted, the distance between the upper vibrating disk 9 and the lower vibrating disk 13 can be changed only by adding the rubber pad 102 in the ring groove 101 or replacing the thickness of the rubber pad 102, the more serious the interference between the protrusions on the upper vibrating disk 9 and the protrusions on the lower vibrating disk 13 is, the larger the amplitude of the water bath barrel 3 is, and otherwise, the smaller the interference between the protrusions on the upper vibrating disk 9 and the protrusions on the lower vibrating disk 13 is, the smaller the amplitude of the water bath barrel 3 is, so that the purpose of adjusting the amplitude is achieved; in addition, the upper and lower bulges are easy to wear due to continuous collision, the upper vibration disc 9 and the lower vibration disc 13 are taken as wearing parts and are detachably connected to the placing frame 12 or the water bath barrel 3, so that the replacement is convenient, and the interchangeability of the upper vibration disc 9 and the lower vibration disc 13 is increased.

The outer sealing connection of casing 1 has heat preservation 5, is the vacuum region between heat preservation 5 and the casing 1, the intercommunication has inlet tube 4 on the lateral wall of casing 1, and inlet tube 4 stretches out heat preservation 5 and with heat preservation 5 sealing connection, the intercommunication has outlet pipe 10 on the bottom surface of casing 1. In water entered into casing 1 through inlet tube 4, the aseptic distilled water of water preferred, after water is heated, set up heat preservation 5 outside casing 1 to prevent that the temperature from scattering and disappearing, guarantee for the oscillation process and last the constant temperature state, emit from outlet pipe 10 after using, outlet pipe 10 establishes inside casing 1, does not remain moisture, in order to avoid the rotten 3 internal environments of bacterial contamination shell water bath bucket that produce of water.

The utility model discloses a sealing device, including casing 1, upper cover 8, the fixed annular protruding edge 6 that is equipped with upwards on the upper surface of casing 1, the external diameter of protruding edge 6 is unanimous with the external diameter of casing 1, and the internal diameter of protruding edge 6 is greater than the internal diameter of casing 1, the diameter of upper cover 8 is unanimous with the internal diameter of protruding edge 6, is equipped with the sealing washer of inlaying on casing 1 on the contact site that upper cover 8 and casing 1 lid closed. Because the upper cover 8 is opened and closed frequently, the structure which is connected with the shell 1 through a bolt or fixedly connected with the shell 1 cannot be set, the shell 1 is provided with the convex edge 6, the upper cover 8 is covered with the shell 1 along the inner diameter of the convex edge 6, the upward convex edge 6 can prevent water in the shell 1 from flowing out, and the covered part is also embedded with a sealing ring to form an inner sealing and an outer sealing.

The convex edge 6 is rotatably connected with a pressure head 7, the upper cover 8 covers the shell 1, and the edge of the upper cover 8 is flush with the convex edge 6. In order to ensure that the upper cover 8 does not shake or break away from the shell 1 in the vibration process, the pressing head 7 is arranged on the convex edge 6 in a rotating mode, one end of the pressing head 7 rotates on the connected convex edge 6, after the upper cover 8 is closed, the other end of the pressing head 7 is rotated to the upper cover 8, and the pressing heads 7 can be multiple in number and press the upper cover 8, so that the purpose of preventing the upper cover 8 from breaking away from the shell 1 is achieved.

The protrusions are of a hemispherical structure. The protrusions of the hemispherical structure are all point contacts when in contact, and have no edges and corners to reduce loss.

The utility model discloses when using, lead to earlier the income water, heat the temperature that adapts to the sample schizolysis according to the sample of difference, then open upper cover 8, place kit 14 on bracing piece 33, close upper cover 8 and compress tightly with pressure head 7, then starter motor drives pivot 11 and rotates and begin the vibration, and the sample in the kit 14 is under even vibration and SDS intensive mixing and schizolysis.

The above-mentioned embodiments are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art without departing from the design concept of the present invention should be included in the protection scope defined by the claims of the present invention.

Claims

1. The utility model provides a kit heating oscillation device, includes casing (1), its characterized in that, fixed heating pipe (2) that are equipped with on the inside wall of casing (1), still fixed protruding annular (101) that are equipped with on the inside wall of casing (1), annular (101) embedded rubber pad (102) that are equipped with, be equipped with open water bath barrel (3) in upper end in casing (1), the last edge of water bath barrel (3) is equipped with extension portion (31) to the evagination, and extension portion (31) are placed on rubber pad (102), seted up a plurality of spout groups on the inside wall of water bath barrel (3), spout group includes two corresponding spouts (32), and sliding connection has same bracing piece (33) between two corresponding spouts (32), and all bracing pieces (33) are all parallel to each other, still seted up a plurality of holes (34) on the lateral wall of water bath barrel (3), and the bottom of water bath barrel (3) can be dismantled and be connected with upper vibration dish (9), go up the lower surface epirelief of vibration dish (9) and be equipped with a plurality of archs, casing (1) bottom is rotated and is connected with pivot (11), and pivot (11) upper end is stretched into casing (1) and fixedly connected with horizontally rack (12), and rack (12) top can be dismantled and be connected with down vibration dish (13), and the upper surface of vibration dish (13) is protruding to be equipped with a plurality of archs down, and protruding equipartition sets up, vibration dish (13) and last vibration dish (9) are interfered the setting down.

2. The kit heating and oscillating device according to claim 1, wherein an insulating layer (5) is hermetically connected to the outer side of the housing (1), a vacuum area is formed between the insulating layer (5) and the housing (1), a water inlet pipe (4) is communicated with the side wall of the housing (1), the water inlet pipe (4) extends out of the insulating layer (5) and is hermetically connected with the insulating layer (5), and a water outlet pipe (10) is communicated with the bottom surface of the housing (1).

3. The reagent box heating and oscillating device according to claim 1, wherein an upper cover (8) is arranged above the casing (1), an annular convex edge (6) is fixedly arranged on the upper surface of the casing (1) upwards, the outer diameter of the convex edge (6) is consistent with the outer diameter of the casing (1), the inner diameter of the convex edge (6) is larger than the inner diameter of the casing (1), the diameter of the upper cover (8) is consistent with the inner diameter of the convex edge (6), and a sealing ring embedded on the casing (1) is arranged on the contact part of the upper cover (8) and the casing (1).

4. The reagent box heating and oscillating device according to claim 3, characterized in that the convex edge (6) is rotatably connected with a pressure head (7), and after the upper cover (8) is covered on the shell (1), the edge of the upper cover (8) is flush with the convex edge (6).

5. The device for heating and shaking a reagent cartridge according to claim 1, wherein the protrusion has a hemispherical structure.