CN211528282U - Detachable agarose gel electrophoresis sample application auxiliary plate - Google Patents
Detachable agarose gel electrophoresis sample application auxiliary plate Download PDFInfo
- Publication number
- CN211528282U CN211528282U CN201922085845.6U CN201922085845U CN211528282U CN 211528282 U CN211528282 U CN 211528282U CN 201922085845 U CN201922085845 U CN 201922085845U CN 211528282 U CN211528282 U CN 211528282U
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- Prior art keywords
- agarose gel
- plate
- gel electrophoresis
- spotting
- positioning
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- 238000000246 agarose gel electrophoresis Methods 0.000 title claims abstract description 20
- 239000011543 agarose gel Substances 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 229920003023 plastic Polymers 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 239000000499 gel Substances 0.000 description 15
- 238000001962 electrophoresis Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 238000010079 rubber tapping Methods 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000005574 cross-species transmission Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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Abstract
The utility model discloses an agarose gel electrophoresis application sample accessory plate of removable fraction, the accessory plate includes the upper plate and the hypoplastron of mutual lock, wherein, the hypoplastron is used for bearing the agarose gel of preparation, the upper plate be provided with one row with the round hole that the last point appearance hole of agarose gel corresponds is connected with miniature hourglass hopper-shaped's application of sample mouth on every round hole. The utility model discloses the design of accessory plate is economic, simple and convenient, has not only kept traditional agarose gel electrophoresis's function to the most common problem of agarose gel electrophoresis has been solved.
Description
Technical Field
The utility model belongs to the technical field of biology, but agarose gel electrophoresis application of sample accessory plate of split type is specifically designed.
Background
Agarose gel electrophoresis is used for identifying, separating and purifying nucleic acids (including DNA and RNA) with various fragment sizes and sometimes viruses according to the principle that nucleic acids with negative charges can migrate to a positive electrode in an electric field by taking agarose as a medium. The method is widely applied in various subject research fields.
At present, the common operation method is to prepare agarose gel by a gel making plate, then remove a comb for making gel, put the gel and the gel making plate into an electrophoresis tank, and require that electrophoresis buffer solution in the electrophoresis tank needs to be submerged in the agarose gel, and then sample the agarose gel in a sample application hole on the gel by using a pipette gun. Thus, a problem commonly encountered by laboratory operators is: 1) the agarose gel belongs to a semisolid, particularly in a liquid environment, a gun head has no force application point during sample application, experimenters lack of experience often have sample application deviation, and cannot accurately inject samples into sample application holes, so that the phenomenon of sample loss or mutual pollution of adjacent samples is caused, and the experimental result is seriously influenced; 2) many times, in order to ensure that a sample is completely injected into a sample application hole, an experimenter inserts a pipette head deeper into the sample application hole, but in this case, the sample is likely to leak out due to the fact that the force is not appropriate and the bottom of the sample application hole is punctured; 3) when the experimenter samples, sometimes can not be fine hold the sample and subside the time to the point appearance hole bottom, therefore, too fast point sample speed can cause some samples to spill over, can influence the concentration of sample equally and cause the pollution of adjacent sample, even to the sample that needs the tapping to retrieve, is a waste.
SUMMERY OF THE UTILITY MODEL
Utility model purpose: in order to solve the technical problem existing in the prior art, the utility model provides a but agarose gel electrophoresis sample application accessory plate of split type.
The technical scheme is as follows: in order to realize the technical purpose, the utility model discloses a but agarose gel electrophoresis application of sample accessory plate of split includes the upper plate and the hypoplastron of mutual lock, wherein, the hypoplastron is used for bearing the agarose gel of preparation, the upper plate be provided with one row with the round hole that the point appearance hole on the agarose gel corresponds is connected with miniature hourglass hopper-shaped sample application mouth on every round hole.
Wherein the diameter of the round hole is 0.5-1 mm smaller than that of the point sample hole.
The diameter of the bottom of the miniature funnel-shaped sample application port is 4-5 mm.
The lower surface of the upper plate is provided with positioning protrusions, the upper surface of the lower plate is provided with positioning grooves corresponding to the positioning protrusions, and the upper plate and the lower plate are positioned and fixed by inserting the positioning protrusions into the positioning grooves.
Preferably, the positioning protrusions and the positioning grooves are respectively disposed around the upper plate and the lower plate. More preferably, positioning protrusions and positioning grooves may be optionally provided at four corners of the upper and lower plates.
In another embodiment, the upper plate and the lower plate are respectively provided with positioning holes corresponding to each other, the inner surfaces of the positioning holes are provided with threads, and the upper plate and the lower plate are fixed by rotating the positioning pins into the positioning holes.
Preferably, the upper and lower plates are made of plastic.
Has the advantages that: compared with the prior art, the utility model has the advantages of as follows:
(1) the design of the miniature funnel-shaped sample application port on the upper plate can provide a stable acting point for sample application, and simultaneously, sample application holes are conveniently found, so that samples are prevented from being polluted by each other, and the problem of sample overflow caused by too high sample application speed can be effectively avoided, and even if the samples are floated upwards instantaneously, the samples can be gradually settled at the bottom of agarose gel under the blocking of the upper plate;
(2) the sample is applied through a micro-funnel and is far away from the agarose gel, so that the possibility that the bottom of the gel is punctured due to improper force application is avoided;
(3) the upper plate and the lower plate are detachably designed, so that the aim of repeated use is fulfilled, loss is basically avoided, only used gel needs to be discarded and cleaned, and the device is economical and simple.
Drawings
FIG. 1 is a side view of an auxiliary plate according to the present invention;
fig. 2 is a plan view of the auxiliary plate of the present invention.
Detailed Description
The technical solution of the present invention will be described in detail by the following specific examples.
As shown in fig. 1-2, the utility model discloses but agarose gel electrophoresis application auxiliary plate of split type includes upper plate 1 and hypoplastron 2 of mutual lock, upper plate 1 and hypoplastron 2 are the plastics material, wherein, hypoplastron 2 is used for bearing the agarose gel of preparation, upper plate 1 is provided with one row and the round hole 4 that point appearance hole 3 on the agarose gel corresponds, be connected with miniature hourglass hopper-shaped sample application mouth 5 on every round hole, the diameter in the diameter ratio point appearance hole of round hole is little 0.5~1mm, the diameter of miniature hourglass hopper-shaped sample application mouth bottom is 4~5 mm.
The upper plate and the lower plate are respectively provided with a corresponding positioning hole 6, the inner surface of the positioning hole is provided with threads, and the upper plate and the lower plate are rotated into the positioning hole through a positioning pin 7 to be fixed. Or optionally, four positioning protrusions are arranged on the periphery of the lower surface of the upper plate, four positioning grooves corresponding to the positioning protrusions are arranged on the upper surface of the lower plate, and the upper plate and the lower plate are positioned and fixed by inserting the positioning protrusions into the positioning grooves.
When the gel fixing device is used, gel is fixed in the lower plate, and after the gel is completely solidified, the upper plate is covered on the gel, is attached to the surface of the gel, and is fixed with the lower plate. Owing to have the existence of upper plate, find the impetus of point sample very easily when the point sample, because micro-funnel's design, find the point sample aperture very easily, the sample can not pollute each other, and simultaneously, because micro-funnel bottom little round hole's diameter is less than the diameter of point sample hole on the agarose gel, just can be fine avoid being used for the point sample speed too fast, the problem that the sample spills over, even if the sample has instantaneous rising, also can settle gradually in the bottom of agarose gel under the blockking of upper plate. Also, because the spotting is through a micro-funnel, away from the agarose gel, there is no possibility of improper force to puncture the bottom of the gel.
On the other hand, the upper plate and the lower plate are designed to be detachable, so that the aim of repeated use is fulfilled, loss is basically avoided, the used gel is only required to be discarded and cleaned, and the method is economical and simple. Meanwhile, for a sample needing tapping recovery, the design is necessary, and the gel after electrophoresis needs to be completely and quickly taken out so as to recover the target band at the fastest speed.
To sum up, the utility model discloses but the agarose gel electrophoresis application of sample accessory plate of split type's design economy, simple and convenient, not only remain traditional agarose gel electrophoresis's function to the most common 3 big problems of agarose gel electrophoresis have been solved.
Claims (7)
1. The utility model provides an agarose gel electrophoresis application of sample accessory plate of removable fraction, its characterized in that, the accessory plate includes upper plate and hypoplastron of mutual lock, wherein, the hypoplastron is used for bearing the agarose gel of preparation, the upper plate seted up one row with the round hole that the point appearance hole on the agarose gel corresponds, every round hole top is connected with miniature hourglass hopper-shaped point sample mouth.
2. The agarose gel electrophoresis spotting auxiliary plate of claim 1, wherein the diameter of the circular hole is 0.5 to 1mm smaller than the diameter of the spotting hole.
3. The agarose gel electrophoresis spotting auxiliary plate of claim 1, wherein the diameter of the bottom of the micro funnel-shaped spotting port is 4-5 mm.
4. The agarose gel electrophoresis spotting auxiliary plate of claim 1, wherein the lower surface of the upper plate is provided with positioning protrusions, the upper surface of the lower plate is provided with positioning grooves corresponding to the positioning protrusions, and the upper plate and the lower plate are positioned and fixed by inserting the positioning protrusions into the positioning grooves.
5. The agarose gel electrophoresis spotting auxiliary plate of claim 4, wherein the positioning protrusions and positioning grooves are respectively provided around the upper plate and the lower plate.
6. The agarose gel electrophoresis spotting auxiliary plate of claim 4, wherein the upper plate and the lower plate are respectively provided with positioning holes corresponding to each other, the inner surfaces of the positioning holes are provided with threads, and the upper plate and the lower plate are fixed by rotating the positioning pins into the positioning holes.
7. The agarose gel electrophoresis spotting auxiliary plate of claim 1, wherein the upper and lower plates are upper and lower plates made of plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922085845.6U CN211528282U (en) | 2019-11-28 | 2019-11-28 | Detachable agarose gel electrophoresis sample application auxiliary plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922085845.6U CN211528282U (en) | 2019-11-28 | 2019-11-28 | Detachable agarose gel electrophoresis sample application auxiliary plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211528282U true CN211528282U (en) | 2020-09-18 |
Family
ID=72464151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922085845.6U Expired - Fee Related CN211528282U (en) | 2019-11-28 | 2019-11-28 | Detachable agarose gel electrophoresis sample application auxiliary plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211528282U (en) |
-
2019
- 2019-11-28 CN CN201922085845.6U patent/CN211528282U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220720 Address after: 036899 Shengli South Road, Pinglu District, Shuozhou City, Shanxi Province (501, unit 2, A2, Nanshan No. 1) Patentee after: Shuozhou Changlin Trading Co.,Ltd. Address before: 030619 Shanxi University of Traditional Chinese Medicine, 121 University Street, College Park, Jinzhong City, Shanxi Province Patentee before: SHANXI University OF CHINESE MEDICINE |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200918 |