CN211367553U - Multi-channel circular liquid drop matrix preparation device special for test tube baby micro-operation vessel - Google Patents
Multi-channel circular liquid drop matrix preparation device special for test tube baby micro-operation vessel Download PDFInfo
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- CN211367553U CN211367553U CN201920642405.3U CN201920642405U CN211367553U CN 211367553 U CN211367553 U CN 211367553U CN 201920642405 U CN201920642405 U CN 201920642405U CN 211367553 U CN211367553 U CN 211367553U
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Abstract
The utility model discloses a multichannel circular liquid drop matrix preparation device special for a test tube baby micro-operation vessel, which comprises a pressure transmission disc, a filter element and a liquid-transfering gun head; the pressure transmission disc is of a flat rectangular structure; the utility model has the advantages that firstly, the matrix preparation device of the utility model is connected with the single-channel liquid shifter, so that the preparation of the often complicated and boring round liquid drops in each IVF laboratory can be easily and conveniently realized, operators can be greatly liberated from the operation of the repeated machines, and more important matters can be made with concentrated attention; secondly, the utility model discloses a matrix preparation ware is synchronous once only to prepare a set of a plurality of circular liquid drops in same operation ware or culture dish, and the water electrolyte osmotic pressure of the same circular liquid drop inside of group in same operation ware or culture dish keeps highly uniform, has guaranteed the accuracy and the repeatability of IVF experiment, and it is great to guarantee IVF experimental result effect high efficiently.
Description
Technical Field
The utility model relates to a medical science and biological technical field especially relate to a circular liquid drop matrix preparation ware of special multichannel of test tube baby micro-operation ware.
Background
The operation of preparing circular liquid drops in a handling dish or a culture dish in the test tube infant technology is one of the most basic, most conventional and most work-intensive operation steps, and the current operation is mainly to perform various types of relevant liquid transfer and prepare various types of operation and culture liquid drops by using a 200ul single-channel manual pipettor (gun) matched with a pipetting plastic gun head, wherein the operation or the culture uses the circular liquid drops, because the using amount is large, 8-10 circular liquid drops are generally required to be prepared in each conventional operation or culture dish, and the number of the handling dishes or the culture dishes required to be used every day in a laboratory with large work capacity is huge, so the work load of preparing the circular liquid drops is more remarkable.
At present, the method is limited by the force of the prior art and the conditions of a matched pipettor and a pipette tip, all the operations are manually single-droplet processing operations each time, 8-10 circular droplets are arranged at one point on an operating dish or a culture dish in a manual method to form 2 rows of rectangular arrays, and the aim of preparing a plurality of droplet operating dishes or culture dishes is fulfilled.
SUMMERY OF THE UTILITY MODEL
1. Technical problem to be solved
The technical problem to be solved by the utility model is to provide a circular liquid drop matrix preparation ware of special multichannel of test tube baby micromanipulation ware, solve the problem that need prepare a large amount of operation dishes or culture dishes that carry 8-10 matrix arrangement circular liquid drops in IVF (in vitro reproduction) scientific research clinical production work, under the existing condition, operating personnel must all cooperate single liquid-transfering gun head through single track manual pipettor, two rows of circular liquid drops of about 4 or 5 uL volume range size are carefully put on each operation dish or culture dish one by one, other operations are cooperated again, corresponding operation dishes or culture dishes are prepared, this method wastes time and energy, under the high flux requirement, physical strength and endurance of operating personnel are serious tests, and the time that these circular liquid drops are prepared has, lead to the internal water electrolyte osmotic pressure between the prepared liquid drops and the like not consistent, the method also causes certain trouble to downstream IVF experiments, influences the accuracy and repeatability of the whole IVF experiment, designs a special multi-channel circular droplet matrix preparation device special for IVF, matches with conventional single-channel manual pipettors with various volumes in an IVF laboratory, the method can synchronously prepare 8-10 circular operations or culture liquid drops meeting requirements in an IVF laboratory at one time, greatly facilitates the preparation of an IVF operation dish or culture dish under the requirement of high flux, ensures the high consistency of the osmotic pressure of the water electrolyte inside a group of circular liquid drops in the same operation dish or culture dish, ensures the strict accuracy and high repeatability of various IVF experiment results, efficiently solves the problem of the preparation of a circular liquid drop matrix operation dish or the high-flux preparation of a culture dish in the IVF experiment under the requirement of high flux, and can be matched with various automatic liquid transfer operation systems.
2. Technical scheme
In order to realize the purpose, the technical scheme of the utility model is that: a multi-channel circular liquid drop matrix preparation device special for a test tube baby micromanipulator comprises a pressure transmission disc, a filter element and a liquid-transfer gun head; the pressure transmission disc is of a flat rectangular structure, the center of the top surface of the pressure transmission disc extends upwards to form a liquid transfer device joint, and the liquid transfer device joint is provided with an air inlet along the axis; the bottom surface of the pressure transmission disc extends at intervals along the axis to form a plurality of pipette tips, and the pipette tips are in a matrix shape along the length and width directions of the pressure transmission disc and are arranged at equal intervals in the length direction and the width direction respectively; a cavity is formed in the pressure transmission disc and is communicated with the air inlet hole and the pipette tips; the filter element is mounted within the cavity.
The special multi-channel circular liquid drop matrix preparation device for the test tube baby micro-operation vessel is characterized in that the pipettor joint is of a hollow circular truncated cone structure with a large upper part and a small lower part, the diameter of the large end is 7mm, the diameter of the small end is 5.5mm, and the wall thickness of the pipettor joint is 1 mm.
The multi-channel circular droplet matrix preparation device special for the test tube baby micromanipulator is characterized in that the upper part of the inner peripheral wall of the pipettor joint extends inwards along the radial direction to form a reinforcing ring.
The circular liquid drop matrix preparation ware of foretell special multichannel of test tube baby micro-manipulation ware, wherein, pipettor connects periphery wall top to be provided with the frosting, just frosting length is 4 mm.
The multi-channel circular droplet matrix preparation device special for the test tube baby micro-manipulation vessel is characterized in that the pressure transmission disc is 20mm in length, 12mm in width and 1mm in thickness; the cavity is of a rectangular structure, the length of the cavity is 18mm, the width of the cavity is 10mm, and the thickness of the cavity is 0.5 mm.
According to the multi-channel circular liquid drop matrix preparation device special for the test tube baby micromanipulation dish, a plurality of pipette tips form a four-row two-column matrix.
The multi-channel circular liquid drop matrix preparation device special for the test tube baby micromanipulator is characterized in that the center distance between two adjacent pipette tips in the same row or the same column is 4 mm.
The multi-channel circular droplet matrix preparation device special for the test tube baby micromanipulator is characterized in that the minimum distance between a plurality of pipette tips and the side line of the pressure transmission disc is 4 mm.
The multi-channel circular liquid drop matrix preparation device special for the test tube baby micro-operation vessel is characterized in that the liquid transfer gun head consists of an upper cylinder and a lower bell mouth; the outer diameter of the cylinder is 4mm, the wall thickness is 0.5mm, and the length is 9 mm; the external diameter of the bottom of the bell mouth is 0.8mm, the wall thickness is 0.2mm, and the length is 1 mm.
3. Advantageous effects
To sum up, the beneficial effects of the utility model reside in that:
(1) the matrix preparation device of the utility model is connected with the single-channel pipettor, so that the preparation work of the often complicated and boring round liquid drops in each IVF laboratory can be easily and conveniently changed, operators can be greatly liberated from the operation of the repeated machines, and the operators can concentrate on doing other more important matters;
(2) the utility model discloses a matrix preparation ware is in step once only to prepare a set of a plurality of circular liquid drops in same operation ware or culture dish, and the water electrolyte osmotic pressure of the circular liquid drop inside of the same group in same operation ware or culture dish keeps highly uniform, has guaranteed the accuracy and the repeatability of IVF experiment, and it is great to guarantee IVF experimental result effect high efficiently.
Drawings
Fig. 1 is a schematic structural view of the multi-channel circular droplet matrix preparation device special for the test tube baby micro-manipulation vessel of the present invention.
FIG. 2 is a sectional view of the multi-channel circular droplet matrix preparation device for the test tube baby micro-manipulation vessel of the present invention.
Detailed Description
The embodiments of the present invention will be further explained with reference to the drawings.
Referring to fig. 1 and 2, a multi-channel circular droplet matrix preparation device special for a test tube baby micromanipulator comprises a pressure transmission disc 1, a filter element 4 and a pipette tip 2; the pressure transmission disc 1 is of a flat rectangular structure, the center of the top surface of the pressure transmission disc 1 extends upwards to form a liquid transfer device joint 3, and the liquid transfer device joint 3 is provided with an air inlet 301 along the axis; the bottom surface of the pressure transmission disc 1 extends at intervals along the axis to form a plurality of pipette tips 2, and the pipette tips 2 are in a matrix shape along the length and width directions of the pressure transmission disc 1 and are arranged at equal intervals in the length direction and the width direction respectively; a cavity 101 is formed in the pressure transmission disc 1, and the cavity 101 is communicated with the air inlet hole 301 and the pipette tips 2; the filter element 4 is mounted in the cavity 101.
The outer contour of the pipettor joint 3 is of a hollow round platform structure with a large upper part and a small lower part, the diameter of the large end is 7mm, the diameter of the small end is 5.5mm, the wall thickness of the pipettor joint is 1mm, and the pipettor joint 3 is connected with a 200uL manual single-channel pipettor and used for transmitting pipetting air pressure generated by the pipettor.
The upper part of the inner peripheral wall of the pipette joint 3 extends inwards along the radial direction to form a reinforcing ring 303, so that the connecting friction force between the pipette joint 3 and a manual single-channel pipette can be effectively increased, and the connecting disc 1 is prevented from sliding off in the pipetting operation process to cause pipetting failure.
The top of the outer peripheral wall of the pipette joint 3 is provided with a frosted surface 302, the length of the frosted surface 302 is 4mm, a hand contact surface is conveniently added under special conditions when the special gun head is manually tightened or removed as required, manual direct operation under the special conditions is facilitated, and marking is also conveniently carried out during operation.
The pressure transmission disc 1 is 20mm in length, 12mm in width and 1mm in thickness; the cavity 102 is a rectangular structure, and has a length of 18mm, a width of 10mm, and a thickness of 0.5 mm.
A plurality of pipette head 2 constitutes four rows and two lists of matrix, and is a plurality of minimum distance between pipette head 2 and the 1 sideline of pressure transmission dish is 4mm, and is with the adjacent two of line or with the line the centre-to-centre spacing of pipette head 2 is 4mm, guarantees that pressure transmission is direct timely even, can be with the liquid-transfering pressure smoothly at the single track pipettor and the supporting a plurality of being connected mutually between the pipette head 2 when liquid-transfering divides the operation to the pressure of 3 inside ends of each pipette head keeps strict synchronization during liquid-transfering operation, ensures that the liquid-transfering volume is unanimous, and good reproducibility.
The pipette tip 2 consists of an upper cylinder 201 and a lower bell mouth 202; the cylinder 201 has an outer diameter of 4mm, a wall thickness of 0.5mm and a length of 9 mm; the external diameter of the bottom of the bell mouth 202 is 0.8mm, the wall thickness is 0.2mm, and the length is 1 mm.
The using method comprises the following steps: the pipette joint is connected with a 200uL Eppendorf single-channel pipette, then the pipette head 2 directly extends out of the pressure transmission disc 1 to carry out 120uL total pipetting volume, 8 circular droplet matrixes are synchronously prepared for example, and meanwhile, the pipette head 2 can provide multiple channels of the type suitable for liquid separation requirements of different measuring range ranges according to different specific applications, and the pipette head 2 is connected with the single-channel pipette.
The above only is the preferred embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structure changes made by the content of the present invention or the direct or indirect application attached to the technical field of other related products are included in the patent protection scope of the present invention.
Claims (9)
1. A special multichannel circular liquid drop matrix preparation device for a test tube baby micro-manipulation vessel is characterized in that: comprises a pressure transmission disc (1), a filter element (4) and a liquid-transfering gun head (2); the pressure transmission disc (1) is of a flat rectangular structure, the center of the top surface of the pressure transmission disc (1) extends upwards to form a liquid transfer device joint (3), and the liquid transfer device joint (3) is provided with an air inlet (301) along the axis; the bottom surface of the pressure transmission disc (1) extends at intervals along the axis to form a plurality of pipette tips (2), and the pipette tips (2) are in a matrix shape along the length and width directions of the pressure transmission disc (1) and are arranged at equal intervals in the length direction and the width direction respectively; a cavity (101) is formed in the pressure transmission disc (1), and the cavity (101) is communicated with the air inlet hole (301) and the pipetting tips (2); the filter element (4) is mounted in the cavity (101).
2. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the outer contour of the liquid transfer device joint (3) is of a hollow round platform structure with a large upper part and a small lower part, the diameter of the large end is 7mm, the diameter of the small end is 5.5mm, and the wall thickness of the liquid transfer device joint is 1 mm.
3. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the upper part of the inner peripheral wall of the pipette joint (3) extends inwards in the radial direction to form a reinforcing ring (303).
4. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the top of the outer peripheral wall of the pipette joint (3) is provided with a frosting surface (302), and the length of the frosting surface (302) is 4 mm.
5. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the length of the pressure transmission disc (1) is 20mm, the width of the pressure transmission disc is 12mm, and the thickness of the pressure transmission disc is 1 mm; the cavity (101) is of a rectangular structure, and is 18mm in length, 10mm in width and 0.5mm in thickness.
6. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the pipette tips (2) form a matrix with four rows and two columns.
7. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the distance between the centers of two adjacent pipette tips (2) in the same row or the same column is 4 mm.
8. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the minimum distance between the pipette tips (2) and the sidelines of the pressure transmission disc (1) is 4 mm.
9. The multi-channel circular droplet matrix preparation device special for the test tube infant micromanipulator of claim 1, wherein: the pipette tip (2) consists of an upper cylinder (201) and a lower bell mouth (202); the outer diameter of the cylinder (201) is 4mm, the wall thickness is 0.5mm, and the length is 9 mm; the external diameter of the bottom of the bell mouth (202) is 0.8mm, the wall thickness is 0.2mm, and the length is 1 mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920642405.3U CN211367553U (en) | 2019-05-07 | 2019-05-07 | Multi-channel circular liquid drop matrix preparation device special for test tube baby micro-operation vessel |
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| CN201920642405.3U CN211367553U (en) | 2019-05-07 | 2019-05-07 | Multi-channel circular liquid drop matrix preparation device special for test tube baby micro-operation vessel |
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| CN211367553U true CN211367553U (en) | 2020-08-28 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988705A (en) * | 2019-05-07 | 2019-07-09 | 刘晓 | A kind of test-tube baby's micromanipulation ware special multichannel circular drop matrix preparing device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109988705A (en) * | 2019-05-07 | 2019-07-09 | 刘晓 | A kind of test-tube baby's micromanipulation ware special multichannel circular drop matrix preparing device |
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