CN212237207U

CN212237207U - DNA synthesis micropore plate

Info

Publication number: CN212237207U
Application number: CN202021974891.8U
Authority: CN
Inventors: 王升启; 彭泳缙; 荣振; 肖瑞; 陈宝财
Original assignee: Institute of Pharmacology and Toxicology of AMMS
Current assignee: Institute of Pharmacology and Toxicology of AMMS; Academy of Military Medical Sciences AMMS of PLA
Priority date: 2020-09-10
Filing date: 2020-09-10
Publication date: 2020-12-29
Anticipated expiration: 2030-09-10

Abstract

The utility model provides a DNA synthesis micropore board relates to biological medicine science technical field. The DNA synthesis microplate comprises a substrate; the base plate comprises an upper surface and a lower surface which are oppositely arranged, a plurality of counter bores are arranged on the upper surface, and a plurality of through holes are arranged at the bottoms of the counter bores. The utility model discloses a DNA synthesis micropore board sets up the counter bore on the plane, and sets up a plurality of through-holes in the bottom of counter bore, can concentrate and pour into a chemical reagent into to a counter bore when need pour into different chemical reagents into in the same batch of reaction process, can realize once only pouring into a chemical reagent's effect into to a plurality of through-holes, has improved chemical reagent's injection efficiency.

Description

DNA synthesis micropore plate

Technical Field

The utility model relates to a biological medicine science technical field particularly, relates to a DNA synthesis micropore board.

Background

De novo synthesis of DNA sequences enables the redesign of biological macromolecules, gene metabolic networks and even entire genomes. Therefore, DNA synthesis supports the development and trend of the field of synthetic biology, and a high-throughput, automatic and low-cost synthetic method is particularly important.

Currently, most oligonucleotides are synthesized by solid phase phosphoramidite chemistry, and the synthesis matrix is usually a multi-well plate containing a reaction carrier. The existing multi-well plates are usually 96-well plates and 384-well plates, and are difficult to meet the requirement of large-scale genome synthesis; in the one-time complete liquid injection process, a plurality of different chemical reagents can be injected, or the same chemical reagent can be injected into all the holes, so that the efficiency of injecting the chemical reagent into the through holes by using one liquid injection spray head or one row of spray heads is lower. Therefore, the miniaturized, compartmentalized design of multiwell plate synthesis sites helps to increase synthesis flux reaction rates.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a DNA synthesis micropore board, it helps solving above-mentioned technical problem.

The utility model discloses a realize like this:

a DNA synthesis microplate comprising a substrate; the base plate comprises an upper surface and a lower surface which are oppositely arranged, a plurality of counter bores are arranged on the upper surface, and a plurality of through holes are formed in the bottoms of the counter bores.

When the DNA synthesis microplate is used, since the operation of injecting the chemical agent into the through-holes is performed by an array of injection nozzles or an ink jet head, it takes a certain period of time to inject the chemical agent into each through-hole. When all the through holes need to be injected with a chemical reagent, the DNA synthesis micropore plate is not much different from the conventional synthesis micropore plate. When all the through holes need to be injected with various chemical reagents, each through hole of the conventional synthesis micro-porous plate still needs to spend one operation time, and in the DNA synthesis micro-porous plate, one chemical reagent is injected into one counter bore, so that the chemical reagents can be injected into the through holes within one operation time, and the injection efficiency of the chemical reagents is greatly improved.

Furthermore, the through hole is a reducing hole; the caliber of at least one position in the through hole is smaller than the caliber of the through hole on the bottom of the counter bore. The technical effects are as follows: and if one of the apertures of the through holes is smaller than the aperture of the through hole at the bottom of the counter bore, the reaction carrier can enter the inlet of the through hole and is clamped at the small aperture, and the reaction of DNA sample synthesis can be carried out in the through hole after the chemical reagent is injected.

Further, along the direction from the upper surface to the lower surface, the through hole comprises a first hole section and a second hole section which are communicated in sequence; the caliber of the first hole section is larger than that of the second hole section. The technical effects are as follows: under the prerequisite of reaction carrier is placed in the realization, design into the structure of two hole sections with the through-hole, the machine-shaping is more convenient.

Further, the connecting surface between the first hole section and the second hole section is funnel-shaped. The technical effects are as follows: the connecting surface is funnel-shaped, so that the caliber of the through hole is gradually changed between the first hole section and the second hole section, the reaction carrier can be correspondingly set to be conical or round table-shaped at the bottom, and the reaction carrier is more stable and is not easy to move and loosen.

Further, the connection surface between the first hole section and the second hole section is an annular plane. The technical effects are as follows: the connecting surface is an annular plane, so that the caliber of the through hole is in a sudden change shape between the first hole section and the second hole section, and the reaction carrier can be correspondingly set into a cylinder, so that the adaptability of the DNA synthesis microporous plate is stronger, and the appearance using requirements of the existing various reaction carriers can be met.

Further, along the direction from the upper surface to the lower surface, the through hole comprises a first hole section, a second hole section and a third hole section which are sequentially communicated; the caliber of the first hole section is larger than that of the second hole section, and the caliber of the third hole section is larger than or equal to that of the second hole section. The technical effects are as follows: similar to the structure with the two hole sections, the diameter of the third hole section arranged in the structure is larger than that of the second hole section, so that the discharge speed can be reduced due to the larger diameter when waste liquid is discharged.

Further, the aperture of the through hole gradually decreases along the direction from the upper surface to the lower surface. The technical effects are as follows: in the structure, the whole through hole is a circular truncated cone-shaped through hole, correspondingly, the reaction carrier is also circular truncated cone-shaped, the tightness of the reaction carrier is higher, and the reaction carriers with different specifications can also be used on the DNA synthesis microporous plate.

Further, the shape of the counter bore is circular or regular polygon. The technical effects are as follows: the round or regular polygonal counter bores have regular shapes, and a plurality of through holes are favorably arranged at the bottoms of the counter bores according to a specific arrangement sequence. Because the liquid injection nozzle injects the chemical reagents according to a specific sequence under the control of a program in the using process, the injection efficiency and the accuracy of the chemical reagents can be improved by the through holes which are regularly distributed.

Further, the through holes are distributed in a row type or in an annular shape at the bottom of the counter bore. The technical effects are as follows: for the same purpose, the distribution of the plurality of through holes should follow a specific sequence. When the counter bores are circular, the through holes can be designed to be distributed annularly, and when the counter bores are regular polygons, the through holes can be designed to be distributed in a row.

Furthermore, a boss is arranged on the lower surface corresponding to the position of the counter bore; the port of the through hole is positioned on the boss. The technical effects are as follows: the exit end of through-hole among the prior art generally is the end of pipeline section, sets up the export of a plurality of through-holes on the boss, then DNA synthesis micropore board production preparation is convenient, and the packing of being convenient for also does benefit to and collects the waste liquid in the exit of through-hole.

The utility model has the advantages that:

the utility model discloses a DNA synthesis micropore board sets up the counter bore on the plane, and sets up a plurality of through-holes in the bottom of counter bore, can concentrate and pour into a chemical reagent into to a counter bore when need pour into different chemical reagents into in the same batch of reaction process, can realize once only pouring into a chemical reagent's effect into to a plurality of through-holes, has improved chemical reagent's injection efficiency.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic view of the internal structure of a DNA synthesis microplate according to a first embodiment of the present invention;

FIG. 2 is a schematic view of the internal structure of a DNA synthesis microplate according to a second embodiment of the present invention;

FIG. 3 is a schematic view of the internal structure of a DNA synthesis microplate according to a third embodiment of the present invention;

FIG. 4 is a schematic view of the internal structure of a DNA synthesis microplate according to a fourth embodiment of the present invention;

FIG. 5 is a schematic diagram of the internal structure of a DNA synthesis microplate according to a fifth embodiment of the present invention;

FIG. 6 is a perspective view of a first combination of shapes of wells and through holes in a DNA synthesis microplate according to any embodiment of the present invention;

FIG. 7 is a top view of a first combination of shapes for wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 8 is a bottom view of a first combination of shapes for wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 9 is a perspective view of a second combination of shapes for wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 10 is a top view of a second combination of shapes for wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 11 is a bottom view of a second combination of shapes for wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 12 is a perspective view of a third combination of shapes of wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 13 is a top view of a third combination of shapes for wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 14 is a bottom view of a third combination of shapes for wells and through holes in a DNA synthesis microplate according to any one of the embodiments of the present invention;

FIG. 15 is a first schematic view of a DNA synthesis microplate provided in the present invention;

FIG. 16 is a second outline view of the DNA synthesis microplate according to the present invention.

Icon: 100-a substrate; 101-upper surface; 102-lower surface; 200-counter bore; 300-a through hole; 301-a first bore section; 302-a second bore section; 303-connecting surface; 304-a third bore section; 400-boss.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. The components of embodiments of the present invention, as generally described and illustrated in the figures herein, can be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the position or positional relationship based on the position or positional relationship shown in the drawings, or the position or positional relationship which is usually placed when the product of the present invention is used, and are only for convenience of description and simplification of the description, but do not indicate or imply that the device or element referred to must have a specific position, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another and are not to be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present invention, it should also be noted that, unless otherwise explicitly specified or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Some embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The embodiments described below and the features of the embodiments can be combined with each other without conflict.

The first embodiment:

FIG. 1 is a schematic diagram of the internal structure of a DNA synthesis microplate according to a first embodiment of the present invention. Referring to fig. 1, the present embodiment provides a DNA synthesis microplate, which includes a substrate 100; the base plate 100 comprises an upper surface 101 and a lower surface 102 which are oppositely arranged, a plurality of counter bores 200 are arranged on the upper surface 101, and a plurality of through holes 300 are arranged at the bottoms of the counter bores 200.

Further, as shown in fig. 1, the through hole 300 is a reducing hole; the aperture of at least one of the through holes 300 is smaller than the aperture of the through hole 300 at the bottom of the counterbore 200.

At this time, the diameter of the through-hole 300 on the bottom of the counterbore 200 is large, and a reaction carrier can be put in, and the diameter of one place inside the through-hole 300 becomes small, so that the reaction carrier can be caught inside the through-hole 300. After injecting the chemical reagent, the reaction of DNA sample synthesis can be performed in the through-hole 300.

Further, as shown in fig. 1, in a direction from the upper surface 101 to the lower surface 102, the through hole 300 includes a first hole section 301 and a second hole section 302 which are sequentially communicated; the aperture of the first hole section 301 is larger than the aperture of the second hole section 302.

Conventional multi-well plates are typically 96-well plates and 384-well plates, and are difficult to meet large-scale genome synthesis requirements. On the other hand, the DNA synthesis microplate according to the present example can be used to produce a plurality of types of multi-well plates:

1. 2400 pore plates, where the pore size of the first pore section 301 is 1 mm and the pore size of the second pore section 302 is 0.8 mm.

2. 3456 orifice plate, wherein the first orifice section 301 has an orifice diameter of 0.8 mm and the second orifice section 302 has an orifice diameter of 0.6 mm.

3. 6144 orifice plate, wherein the first orifice section 301 has an orifice diameter of 0.6 mm and the second orifice section 302 has an orifice diameter of 0.4 mm.

4. 13824 orifice plate: wherein the first hole section 301 has a hole diameter of 0.4 mm and the second hole section 302 has a hole diameter of 0.3 mm.

In this structure, on the premise of placing the reaction carrier, the through hole 300 is designed into a structure with two hole sections, so that the processing and molding are more convenient.

Further, as shown in fig. 1, a connection surface 303 between the first hole section 301 and the second hole section 302 is funnel-shaped.

In this structure, connecting face 303 is hopper-shaped and makes the bore of through-hole 300 be the gradual change form between first hole section 301 and second hole section 302, and the reaction carrier also can set to the bottom correspondingly and be coniform or round platform form, and the reaction carrier is placed more firmly and is difficult for the activity pine taking off.

It should be noted that the counterbore 200 and the through hole 300 at the bottom of the counterbore 200 may be selected as desired. The counter bore 200 may be provided in a circular shape and the through-hole 300 may be provided in a square shape, the counter bore 200 may be provided in a square shape and the through-hole 300 may be provided in a circular shape, or both the counter bore 200 and the through-hole 300 may be provided in a circular shape or a square shape.

Among them, the substrate 100 should preferably be provided as a rectangular flat plate, and one of four corners of the rectangular flat plate should be provided with a missing structure to determine the orientation of the rectangular flat plate. And the counter bores 200 on the substrate 100 are arranged in a plurality and are regularly distributed in a row.

The working principle and the operation method of the DNA synthesis microplate are as follows:

since the operation of injecting the chemical into the through-holes 300 is performed by an array of injection nozzles or an inkjet head, it takes a certain period of operation time to inject the chemical into each of the through-holes 300. When all the through-holes 300 need to be filled with a chemical, the DNA synthesis microplate of the present embodiment is not much different from the conventional synthesis microplate. When a plurality of chemical reagents need to be injected into all the through holes 300, each through hole 300 of the conventional synthesis microplate still needs to spend one operation time, and in the DNA synthesis microplate of the present embodiment, one chemical reagent is injected into one counter bore 200, so that the chemical reagents can be injected into the plurality of through holes 300 within one operation time, thereby greatly improving the injection efficiency of the chemical reagents.

Second embodiment:

FIG. 2 is a schematic diagram of the internal structure of a DNA synthesis microplate according to a second embodiment of the present invention. Referring to fig. 2, the present embodiment provides a DNA synthesis microplate, which is substantially the same as the DNA synthesis microplate of the first embodiment, and the difference between the two embodiments is that the connection surface 303 between the first well section 301 and the second well section 302 in the DNA synthesis microplate of the present embodiment is an annular plane.

In the DNA synthesis microplate of the embodiment, the connecting surface 303 in the through hole 300 is an annular plane, so that the caliber of the through hole 300 is in a shape of sudden change between the first hole section 301 and the second hole section 302, and the reaction carrier can be correspondingly set into a cylinder, so that the DNA synthesis microplate has stronger adaptability and can meet the use requirements of the appearance of the existing various reaction carriers.

The third embodiment:

FIG. 3 is a schematic diagram of the internal structure of a DNA synthesis microplate according to a third embodiment of the present invention. Referring to fig. 3, the present embodiment provides a DNA synthesis microplate, which is substantially the same as the DNA synthesis microplate of the first embodiment or the second embodiment, and the difference between the two embodiments is that in the DNA synthesis microplate of the present embodiment, along the direction from the upper surface 101 to the lower surface 102, the through hole 300 includes a first hole section 301, a second hole section 302, and a third hole section 304 that are sequentially connected; the aperture of the first hole section 301 is larger than the aperture of the second hole section 302, and the aperture of the third hole section 304 is larger than or equal to the aperture of the second hole section 302.

The DNA synthesis microplate of the present embodiment, similar to the above-described structure having two well sections, has a larger aperture of the third well section 304 than that of the second well section 302, and thus can reduce the discharge rate when discharging waste liquid because the aperture becomes larger.

The fourth embodiment:

FIG. 4 is a schematic diagram of the internal structure of a DNA synthesis microplate according to a fourth embodiment of the present invention. Referring to fig. 4, the present embodiment provides a DNA synthesis microplate which is substantially the same as the DNA synthesis microplates of the first to third embodiments, and the difference between the two embodiments is that the aperture of the through hole 300 in the DNA synthesis microplate of the present embodiment gradually decreases along the direction from the upper surface 101 to the lower surface 102.

In the DNA synthesis microplate of the present embodiment, the whole through hole 300 is a circular truncated cone-shaped through hole 300, and accordingly, the reaction carrier is also circular truncated cone-shaped, the fastening performance of the reaction carrier is also higher, and the reaction carriers of different specifications can also be used in the DNA synthesis microplate.

On the basis of any of the above embodiments, further, as shown in fig. 4, the shape of the counterbore 200 is a circle or a regular polygon. At this time, the circular or regular polygonal counter bores 200 have a regular shape, and it is advantageous to arrange a plurality of through holes 300 at the bottom of the counter bores 200 in a specific arrangement order. Since the liquid injection nozzle injects the chemical reagents in a specific sequence under the control of a program in the using process, the through holes 300 which are regularly distributed can improve the injection efficiency and accuracy of the chemical reagents.

Fifth embodiment:

FIG. 5 is a schematic diagram of the internal structure of a DNA synthesis microplate according to a fifth embodiment of the present invention. Referring to fig. 5, the present embodiment provides a DNA synthesis microplate, which is substantially the same as the DNA synthesis microplate of any one of the above embodiments, and the difference between the two embodiments is that a boss 400 is disposed on the lower surface 102 of the DNA synthesis microplate of the present embodiment, at a position corresponding to the counterbore 200; the port of the through-hole 300 is located on the boss 400.

Wherein, the exit end of through-hole 300 among the prior art generally is the end of a pipeline section, sets up the export of a plurality of through-holes 300 on boss 400, then DNA synthesis micropore board production preparation is convenient, the packing of being convenient for, also does benefit to and collects the waste liquid in the exit of through-hole 300.

Further, fig. 6 is a perspective view of a first shape combination of the counter bore 200 and the through hole 300 in the DNA synthesis microplate according to any embodiment of the present invention; FIG. 7 is a top view of a first combination of shapes of wells 200 and through holes 300 in a DNA synthesis microplate according to any one of the embodiments of the present invention; FIG. 8 is a bottom view of a first combination of shapes of wells 200 and through holes 300 in a DNA synthesis microplate according to any one of the embodiments of the present invention; FIG. 9 is a perspective view of a second combination of shapes of the wells 200 and the through holes 300 of the DNA synthesis microplate according to any one of the embodiments of the present invention; FIG. 10 is a top view of a second combination of shapes of wells 200 and through holes 300 in a DNA synthesis microplate according to any one of the embodiments of the present invention; FIG. 11 is a bottom view of a second combination of shapes of wells 200 and through holes 300 in a DNA synthesis microplate according to any one of the embodiments of the present invention; FIG. 12 is a perspective view of a third combination of shapes of the wells 200 and the through holes 300 of the DNA synthesis microplate according to any one of the embodiments of the present invention; FIG. 13 is a top view of a third combination of shapes of the wells 200 and the through holes 300 in a DNA synthesis microplate according to any embodiment of the present invention; FIG. 14 is a bottom view of a third combination of shapes of the wells 200 and the through holes 300 in the DNA synthesis microplate according to any embodiment of the present invention. As shown in fig. 6 to 14, the counter bore 200 and the through hole 300 may be designed as regular polygonal holes such as circular holes, square holes or hexagonal holes, as required. Regardless of the shape of the counterbore 200, the plurality of through holes 300 may be distributed in a row-by-row manner, in a ring-shaped manner, or in a honeycomb structure at the bottom of the counterbore 200.

In addition, FIG. 15 is a first external view of the DNA synthesis microplate provided by the present invention; FIG. 16 is a second outline view of the DNA synthesis microplate according to the present invention. As shown in fig. 15 and 16, a threaded hole or an insertion rail or a guide groove should be formed at the bottom of the substrate 100 to achieve the mounting and fixing of the substrate 100 and the working platform.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A DNA synthesis microplate comprising a substrate (100); the substrate (100) comprises an upper surface (101) and a lower surface (102) which are oppositely arranged, a plurality of counter bores (200) are arranged on the upper surface (101), and a plurality of through holes (300) are arranged at the bottoms of the counter bores (200).

2. The DNA synthesis microplate according to claim 1, wherein the through-hole (300) is a variable diameter hole; the caliber of at least one position in the through hole (300) is smaller than the caliber of the through hole (300) on the bottom of the counter bore (200).

3. The DNA synthesis microplate according to claim 2, wherein the through-hole (300) comprises a first well section (301) and a second well section (302) which are connected in series in a direction from the upper surface (101) to the lower surface (102); the aperture of the first hole section (301) is larger than the aperture of the second hole section (302).

4. The DNA synthesis microplate according to claim 3, wherein the connection surface (303) between the first well section (301) and the second well section (302) is funnel-shaped.

5. The DNA synthesis microplate according to claim 3, wherein the connection surface (303) between the first well section (301) and the second well section (302) is an annular plane.

6. The DNA synthesis microplate according to claim 2, wherein the through-hole (300) comprises a first well section (301), a second well section (302) and a third well section (304) in communication in this order in a direction from the upper surface (101) to the lower surface (102); the caliber of the first hole section (301) is larger than that of the second hole section (302), and the caliber of the third hole section (304) is larger than or equal to that of the second hole section (302).

7. The DNA synthesis microplate according to claim 2, wherein the bore of the through-hole (300) becomes gradually smaller in a direction from the upper surface (101) toward the lower surface (102).

8. The DNA synthesis microplate according to any one of claims 1 to 7, wherein the shape of the counter bore (200) is a circle or a regular polygon.

9. The DNA synthesis microplate according to claim 8, wherein a plurality of the through-holes (300) are arranged in a row or in a ring at the bottom of the wells (200).

10. The DNA synthesis microplate according to claim 1, wherein a boss (400) is provided on the lower surface (102) at a position corresponding to the counterbore (200); the port of the through hole (300) is positioned on the boss (400).