CN211329854U

CN211329854U - Special ultracentrifuge for exosome extraction and separation

Info

Publication number: CN211329854U
Application number: CN201921990798.3U
Authority: CN
Inventors: 陈锦阳; 乔博超; 刘军权
Original assignee: Zhejiang Healthfuture Biomedical Technology Co ltd
Current assignee: Zhejiang Healthfuture Biomedical Technology Co ltd
Priority date: 2019-11-18
Filing date: 2019-11-18
Publication date: 2020-08-25
Anticipated expiration: 2029-11-18

Abstract

The utility model relates to the technical field of exosome extraction and separation, in particular to a special ultracentrifuge for exosome extraction and separation, which comprises a box body, wherein a transparent glass window is arranged at the central position of a box cover, a thermometer is arranged on a fixed plate, a semiconductor refrigerating sheet is arranged at the central position on the right side surface of the inner part of the box body, a centrifugal mechanism is arranged at the central position of the inner part of the box body, the centrifugal mechanism comprises an electric telescopic rod, the electric telescopic rod is fixedly connected with the bottom of the box body, a base is arranged at the top end of the electric telescopic rod, a cylinder is arranged on the base, a high-speed motor is arranged inside the cylinder, an output; this special ultracentrifuge of exosome extraction separation sets up transparent glass window person of facilitating the use and observes inside centrifugal state, and semiconductor refrigeration piece carries out inside intensification or cooling, helps the centrifugation, and electric telescopic handle adds establishes, is convenient for extend the box with whole centrifugal mechanism, and person of facilitating the use lays the centrifuging tube.

Description

Special ultracentrifuge for exosome extraction and separation

Technical Field

The utility model relates to an exosome draws separation technical field, specifically speaking relates to a special ultracentrifuge of exosome draws separation.

Background

Exosomes are usually minute membrane vesicles of 30-150 nm, have lipid bilayer structures, are derived from the luminal membrane of the multivesicular body in almost all normal cells and tumor cells, and are thought to participate in communication between cells by loading proteins, metabolites and nucleic acids, etc. from original cells, which can be released into extracellular space after fusion with cell membranes, and many cell surface membrane proteins can be detected on exosomes, some of which are commonly used for early detection, diagnosis and prognosis of cancer, are ultracentrifuges, which are very time-consuming and labor-consuming and require an ultracentrifuge;

among the prior art, ultracentrifuge constructs complicacy, and the cost is higher, and most use of being not convenient for, and the exosomal extraction and separation is consuming time and is used hard, carries out centrifugal separation under the different temperatures and draws, and it is more convenient to draw, and general ultracentrifuge is laying the centrifuging tube inconvenient, and is difficult for letting the user observe centrifugal situation of change.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a special ultracentrifuge of exosome extraction and separation to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme:

an ultracentrifuge special for exosome extraction and separation comprises a box body, wherein the interior of the box body is hollow, a box cover is hinged to the top of the box body, a transparent glass window is arranged at the center position of the box cover, a fixed plate is arranged on the left side face of the interior of the box body, a thermometer is arranged on the fixed plate, a semiconductor refrigerating sheet is arranged at the center position of the right side face of the interior of the box body, heat conducting blocks are connected to two sides of the semiconductor refrigerating sheet, a centrifugal mechanism is arranged at the center position of the interior of the box body and comprises an electric telescopic rod, the electric telescopic rod is fixedly connected with the bottom of the box body, a base is arranged at the top end of the electric telescopic rod, a cylinder is arranged on the base, a high-speed motor is arranged in the cylinder, an output shaft of the high, the rotating shaft is sleeved with a tray, and the upper surface of the tray is provided with a plurality of arc-shaped grooves.

Preferably, the transparent glass window is fixedly bonded with the case cover, and a circle of rubber pad is arranged on the lower surface of the case cover, so that a user can conveniently observe the centrifugal condition through the transparent glass window and can observe the change condition of the thermometer.

Preferably, the fixing plate and the box body are of an integrally formed structure, and the thermometer is fixedly bonded with the fixing plate, so that a user can conveniently and timely master the change of the internal temperature, and the temperature can be adjusted better.

Preferably, the semiconductor refrigeration piece passes through bolt fixed connection with the box, the semiconductor refrigeration piece passes through bolt fixed connection with the heat conduction piece, and the adding of semiconductor refrigeration piece is established, and the inside temperature variation condition of user's hand control box helps the better exosome of carrying on to draw the separation.

As preferred, electric telescopic handle passes through bolt fixed connection with the box, electric telescopic handle passes through bolt fixed connection with the base, and electric telescopic handle adds and establishes, lets whole centrifugal mechanism extend the box, and the user of being convenient for is better lays the centrifuging tube, convenient operation.

Preferably, the base is fixedly connected with the cylinder through bolts, the high-speed motor is fixed with the cylinder through bolts, the rotating shaft and the disc are of an integrally formed structure, and the tray and the rotating shaft are of an integrally formed structure, so that the mounting and dismounting are convenient, and the stability is realized.

As preferred, the through-hole is annular equidistant distribution, and has eight in quantity, the arc wall is annular equidistant distribution, and has eight in quantity, the position one-to-one of through-hole and arc wall position, through-hole and arc wall correspond, and the centrifuging tube of being convenient for lays, prevents in the centrifugation, breaks away from centrifugal mechanism, influences the experiment.

Compared with the prior art, the beneficial effects of the utility model are that:

the utility model discloses a set up transparent glass window person of facilitating the use and observe inside centrifugal state, in time observe the change condition of inside thermometer, person of facilitating the use adjusts the semiconductor refrigeration piece and heaies up or cool down, the setting of heat conduction piece is convenient for even arrive the heat, let inside be heated evenly, be convenient for better carry out exosome and draw the separation, electric telescopic handle adds establishes, be convenient for extend the box with whole centrifugal mechanism, person of facilitating the use lays the centrifuging tube, and simultaneously, when not touching the centrifuging tube, the user can see centrifugal situation, the construction cost is low, and convenient for operation.

Drawings

FIG. 1 is a schematic view of the whole structure of an ultracentrifuge for exosome extraction and separation of the present invention;

FIG. 2 is a schematic view of the special ultracentrifuge for exosome extraction and separation of the present invention in an open state;

FIG. 3 is a schematic structural view of a de-centrifugation mechanism of the present invention;

FIG. 4 is a schematic structural view of a centrifugal mechanism of the present invention;

FIG. 5 is a schematic view of the centrifugal mechanism removing centrifuge tube of the present invention;

fig. 6 is an exploded view of the centrifugal mechanism of the present invention.

In the figure:

1. a box body; 10. a fixing plate;

2. a box cover;

3. a transparent glass window;

4. a thermometer;

5. a centrifugal mechanism; 50. an electric telescopic rod; 51. a base; 52. a cylinder; 520. a high-speed motor; 53. a tray; 530. an arc-shaped slot; 54. a disc; 540. a through hole; 55. a rotating shaft;

6. a semiconductor refrigeration sheet; 60. a heat conducting block;

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Referring to fig. 1-6, the present invention provides a technical solution:

a special ultracentrifuge for exosome extraction and separation comprises a box body 1, the interior of the box body 1 is hollow, a box cover 2 is hinged to the top of the box body 1, a transparent glass window 3 is arranged at the center of the box cover 2, a fixing plate 10 is arranged on the left side face of the interior of the box body 1, a thermometer 4 is arranged on the fixing plate 10, a semiconductor refrigerating sheet 6 is arranged at the center of the right side face of the interior of the box body 1, heat conducting blocks 60 are connected to two sides of the semiconductor refrigerating sheet 6, a centrifugal mechanism 5 is arranged at the center of the interior of the box body 1, the centrifugal mechanism 5 comprises an electric telescopic rod 50, the electric telescopic rod 50 is fixedly connected with the bottom of the box body 1, a base 51 is arranged at the top end of the electric telescopic rod 50, a cylinder 52 is arranged on the base 51, a high-, the disc 54 is provided with a plurality of through holes 540, the tray 53 is sleeved in the middle of the rotating shaft 55, and the upper surface of the tray 53 is provided with a plurality of arc-shaped grooves 530.

In this embodiment, transparent glass window 3 and case lid 2 bond fixedly, and the lower surface of case lid 2 still is equipped with the round rubber pad, and convenient to use person sees through transparent glass window 3 and observes the centrifugal condition, can also observe the change condition of thermometer 4, and when closed case lid 2, case lid 2 can closely laminate with box 1, and the rubber pad increases its gas tightness, prevents that external disturbance from arriving inside.

Secondly, the fixed plate 10 and the box body 1 are of an integrally formed structure, and the thermometer 4 is fixedly bonded with the fixed plate 10, so that a user can conveniently and timely master the change of the internal temperature, and the temperature can be adjusted better.

Further, semiconductor refrigeration piece 6 passes through bolt fixed connection with box 1, and semiconductor refrigeration piece 6 passes through bolt fixed connection with heat conduction piece 60, and adding of semiconductor refrigeration piece 6 establishes, and the inside temperature variation condition of convenient to use person's manual going control box 1 helps better carrying out the exosome and draws the separation, and semiconductor refrigeration piece 6 is connected with external power, and the user can be through changing positive negative pole, control the cooling of semiconductor refrigeration piece 6 and rising the temperature.

Specifically, electric telescopic handle 50 passes through bolt fixed connection with box 1, and electric telescopic handle 50 passes through bolt fixed connection with base 51, and adding of electric telescopic handle 50 establishes, lets whole centrifugal mechanism 5 extend box 1, and the better centrifuging tube of laying of the user of being convenient for, convenient operation, electric telescopic handle 50 external power source user manual control is flexible.

It should be noted that the base 51 is fixedly connected with the cylinder 52 through bolts, the high-speed motor 520 is fixed with the cylinder 52 through bolts, the rotating shaft 55 and the disc 54 are integrally formed, and the tray 53 and the rotating shaft 55 are integrally formed, so that the mounting and dismounting are facilitated, and the stability is achieved.

Further, the through-hole 540 is the equidistant distribution of annular, and has eight in quantity, and the arc wall 530 is the equidistant distribution of annular, and has eight in quantity, and the position of through-hole 540 and the position one-to-one of arc wall 530, through-hole 540 corresponds with arc wall 530, and the centrifugal tube of being convenient for lays, prevents in the centrifugation, breaks away from centrifugal mechanism 5, influences the experiment, and through-hole 540 inclines 30 degrees, the better centrifugation of being convenient for.

It is worth supplementing that the electric compression rod 50 can be 38STG-50-220V electric compression rod manufactured by Wenzhou Simmons Motor Co., Ltd, and the high-speed motor 520 can be HSP-3000r-220V three-phase induction high-speed motor manufactured by Shanghai Yansen manufacturing Co., Ltd; the external power supply carries out alternating current power supply, and a matched circuit and a power supply are also provided by the manufacturer; furthermore, the circuits and electronic components and modules involved in the present invention are prior art and can be implemented by those skilled in the art without further elaboration, nor is the present invention directed to improvements in software and methods.

When the special ultracentrifuge for extracting and separating exosome of the embodiment is used, a user firstly manually fixedly connects the electric telescopic rod 50 with the box body 1 through a bolt, the base 51 is fixedly connected with the electric telescopic rod 50 through a bolt, the high-speed motor 520 is fixed in the cylinder 52 through a bolt, the cylinder 52 is fixedly connected with the base 51 through a bolt, the semiconductor refrigeration sheet 6 is fixedly connected with the box body 1 through a bolt, the thermometer 4 is installed on the fixing plate 10, the semiconductor refrigeration sheet 6 is externally connected with a power supply, the high-speed motor 520 is externally connected with a power supply, the user installs the centrifugal tube in the through hole 540, the bottom of the centrifugal tube is tightly contacted with the arc-shaped groove 530, the box cover 2 is closed, the user manually controls the positive and negative contacts, through observing the change of the thermometer 4, a user can regulate and control the temperature according to different conditions, so that the centrifugation is better performed.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the description in the above embodiments and the description is only preferred examples of the present invention, and is not intended to limit the present invention, and that the present invention can have various changes and modifications without departing from the spirit and scope of the present invention, and these changes and modifications all fall into the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a special ultracentrifuge of exosome extraction separation, includes box (1), its characterized in that: the improved refrigerator is characterized in that the interior of the box body (1) is hollow, the top of the box body (1) is hinged with a box cover (2), a transparent glass window (3) is arranged at the center of the box cover (2), a fixing plate (10) is arranged on the left side face of the interior of the box body (1), a thermometer (4) is arranged on the fixing plate (10), a semiconductor refrigerating sheet (6) is arranged at the center of the right side face of the interior of the box body (1), heat conducting blocks (60) are connected to the two sides of the semiconductor refrigerating sheet (6), a centrifugal mechanism (5) is arranged at the center of the interior of the box body (1), the centrifugal mechanism (5) comprises an electric telescopic rod (50), the electric telescopic rod (50) is fixedly connected with the bottom of the box body (1), a base (51) is arranged at the top end of the electric telescopic rod (50), a cylinder (52), the high-speed motor (520) is characterized in that an output shaft penetrates through the cylinder (52), the output shaft is coaxially connected with a rotating shaft (55), a disc (54) is arranged at the top of the rotating shaft (55), a plurality of through holes (540) are formed in the disc (54), a tray (53) is sleeved in the middle of the rotating shaft (55), and a plurality of arc-shaped grooves (530) are formed in the upper surface of the tray (53).

2. The ultracentrifuge for exosome extraction and separation according to claim 1, characterized in that: the transparent glass window (3) is fixedly bonded with the case cover (2), and a circle of rubber pad is arranged on the lower surface of the case cover (2).

3. The ultracentrifuge for exosome extraction and separation according to claim 1, characterized in that: the fixing plate (10) and the box body (1) are of an integrally formed structure, and the thermometer (4) is fixedly bonded with the fixing plate (10).

4. The ultracentrifuge for exosome extraction and separation according to claim 1, characterized in that: the semiconductor refrigeration piece (6) is fixedly connected with the box body (1) through bolts, and the semiconductor refrigeration piece (6) is fixedly connected with the heat conducting block (60) through bolts.

5. The ultracentrifuge for exosome extraction and separation according to claim 1, characterized in that: the electric telescopic rod (50) is fixedly connected with the box body (1) through bolts, and the electric telescopic rod (50) is fixedly connected with the base (51) through bolts.

6. The ultracentrifuge for exosome extraction and separation according to claim 1, characterized in that: the base (51) is fixedly connected with the cylinder (52) through bolts, the high-speed motor (520) is fixed with the cylinder (52) through bolts, the rotating shaft (55) and the disc (54) are of an integrally formed structure, and the tray (53) and the rotating shaft (55) are of an integrally formed structure.

7. The ultracentrifuge for exosome extraction and separation according to claim 1, characterized in that: the through holes (540) are distributed in an annular equidistant mode and are eight in number, the arc-shaped grooves (530) are distributed in an annular equidistant mode and are eight in number, and the positions of the through holes (540) correspond to the positions of the arc-shaped grooves (530) one by one.