CN211292434U - Device for testing fat content in aquatic organism - Google Patents
Device for testing fat content in aquatic organism Download PDFInfo
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- CN211292434U CN211292434U CN201922075725.8U CN201922075725U CN211292434U CN 211292434 U CN211292434 U CN 211292434U CN 201922075725 U CN201922075725 U CN 201922075725U CN 211292434 U CN211292434 U CN 211292434U
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Abstract
The utility model discloses a device of fat content in test aquatic organism, including tissue homogenate and reagent conveying system, filtration and extraction system, heating and reagent recovery system, weighing system and automatic control screen display system. The utility model discloses an instrument equipment expense high in the past and consuming time, loaded down with trivial details fat extraction method in the past are changed, provide technical support for confirming aquatic organism internal fat content.
Description
Technical Field
The utility model relates to a technical field is formulated to the healthy water environment quality benchmark of human body, and more specifically the device that relates to a test aquatic organism internal fat content that says so.
Background
The fat in the fresh water aquatic organisms contains a large amount of unsaturated fatty acid with high nutritive value, and the unsaturated fatty acid has various physiological functions of regulating blood fat, clearing thrombus, resisting inflammation and the like, and can also tonify brain, strengthen the immunity of the organism and the like; increasing the intake of aquatic organism fat, promoting growth and development, reducing the occurrence of diseases and being beneficial to the development of human health. However, contaminants, particularly nonionic organic substances, are easily concentrated and accumulated in the fat of aquatic organisms, and thus various kinds of pollutants are also taken into the human body along with the intake of the fat of aquatic organisms.
Along with the improvement of living standard, people pay more and more attention to the aspect of dietetic nutrition and health, the demand for the ingestion of aquatic organisms containing fat is larger and larger, and the intake risk of corresponding pollutants is higher and higher. Therefore, the research and the establishment of the water quality standard for protecting the human health are carried out in China, the national standard is issued, and however, in the aspect of fat content parameters of edible aquatic organisms related to the human health standard, fat content parameters, test methods and test devices of various directly related aquatic organisms are lacked. Meanwhile, fat in aquatic organisms is influenced by various factors, not only is related to geographical positions, but also is related to food habits, seasons, climate changes and fish types, so that data of the fat content of the aquatic organisms in various regions are needed for establishing human health standards, and a large amount of testing work of the fat content of the aquatic organisms is also needed.
Therefore, how to provide a device for conveniently, quickly, simply and efficiently testing the fat content in aquatic organisms is a problem to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model discloses an it is expensive in the past to change the instrument and equipment expense and consuming time in the past, loaded down with trivial details fat extraction method, provide a device of full-automatic test aquatic organism internal fat content, provide the basis for confirming aquatic organism internal fat content.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
an apparatus for measuring the fat content in an aquatic organism, comprising
A tissue homogenization and reagent delivery system comprising a reagent storage device, a homogenizer, and an induction moisture meter, the reagent storage device being in communication with the homogenizer via a conduit, and the induction moisture meter being located within the homogenizer;
the filtering and extracting system comprises a porous funnel and a separating funnel, the porous funnel is positioned at the bottom of the homogenizer, and the separating funnel is connected with a liquid outlet of the porous funnel;
the heating and reagent recovery system comprises an extraction cup, a reagent recovery device, a heating device and a condensing device, wherein the extraction cup is positioned at the liquid outlet of the separating funnel, the extraction cup is connected with the reagent recovery device through a pipeline, and the reagent recovery device is communicated with the reagent storage device;
a weighing system for weighing the organisms and the extraction cup;
and the automatic control screen display system is used for setting parameters and specific operation processes required by the whole system and receiving and displaying the measurement results.
Preferably, in the above apparatus for testing fat content in aquatic organisms, the reagent storage means is composed of three storage bottles for storing distilled water, chloroform and methanol, respectively, and the three storage bottles are all communicated to the homogenizer through pipes.
The beneficial effects of the above technical scheme are: the reagent storage device can be replaced by other solvents such as ether or petroleum ether according to actual conditions, the system does not need to be set under the condition of standard reagents (namely distilled water, chloroform and methanol), the test can be automatically completed, and when other reagents are used, the test can be completed after the automatic control screen display system is reset. The three reagents are selected to have better extraction effect, so that the fat is extracted more cleanly, and the fat loss is reduced.
Preferably, in the above apparatus for testing fat content in aquatic organisms, a nozzle is disposed at one end of the pipeline located at the homogenizer, and the nozzle is connected with three conduits controlled by valves, corresponding to the three storage bottles.
The beneficial effects of the above technical scheme are: according to the set liquid adding volume, the spray head can spray corresponding solvent amount into the refiner according to the set experimental solvent adding proportion, and the four walls and the bottom of the refiner are washed.
Preferably, in the above apparatus for testing the fat content in an aquatic organism, a stirring blade is provided in the homogenizer.
The beneficial effects of the above technical scheme are: the stirring tool bit stirs the fish body according to the experiment to the rotational speed of stirring tool bit can be adjusted through system's setting.
Preferably, in the apparatus for measuring fat content in an aquatic organism as described above, the probe of the induction type moisture meter is placed at a middle position of the bottom of the mixer head, and the probe is fixed while being attached to the mixer head.
The beneficial effects of the above technical scheme are: along with the rotation of the stirring tool bit, the probe of the induction type moisture tester can automatically extend to aquatic organism tissues, detect the moisture of the aquatic organism tissues and display the moisture on the automatic control screen display system.
Preferably, in the above apparatus for measuring the fat content in aquatic organisms, the bottom of the homogenizer is composed of two stainless steel semicircles, and the opening and closing of the two stainless steel semicircles are automatically performed by system control.
The beneficial effects of the above technical scheme are: after homogenization, the two semicircles automatically separate to two sides according to an electric signal, aquatic organism tissues and liquid can flow into a funnel of a filtering and extracting system at the bottom, and the two semicircles are closed immediately.
Preferably, in the above apparatus for testing fat content in aquatic organisms, a sensor for controlling the opening and closing of the separating funnel and a detection rod for detecting liquid change are arranged at the glass piston of the separating funnel.
The beneficial effects of the above technical scheme are: separating funnel divide into chloroform and methyl alcohol two-layerly, and glass piston can control separating funnel's switch according to the sensor, and test probe can detect the transform of two kinds of liquid, and when lower liquid flowed into in the extraction cup and flowed, when the methyl alcohol liquid appeared, glass piston self-closing, when weighing the back to the extraction cup, the glass stopper was opened automatically and is released methyl alcohol.
The method for testing the fat content in the aquatic organisms by the device comprises the following steps:
(1) putting the processed aquatic organism tissues into a weighing system to weigh the aquatic organism tissues, and displaying the weighed value at the position of the organism weight in the automatic control screen display system;
(2) feeding the aquatic organism tissue into a homogenizer, setting parameters according to an automatic control screen display system, controlling and opening an induction type moisture meter through the automatic control screen display system to measure the moisture content of the aquatic organism tissue, and further automatically calculating the moisture content of aquatic organisms according to the weight and the moisture content of the aquatic organism tissue;
(3) adding distilled water, chloroform and methanol into a homogenizer according to the water content of aquatic organisms for homogenizing, wherein the extraction method of chloroform-methanol mixed liquor can well extract the combined lipid from tissues, and is particularly suitable for polar fat food containing more phospholipids;
(4) the homogenized material flows onto a porous funnel with filter paper from the bottom, the material is pumped and filtered by a pump, and the liquid flowing out of the porous funnel directly flows into a separating funnel at the lower part, and the liquid is kept stand and layered;
(5) the lower chloroform layer flows into an extraction cup, chloroform is heated and evaporated, chloroform gas flows into a reagent recovery device through a conduit, when the system detects that no chloroform flows into the conduit, the extraction cup is conveyed to a weighing system for weighing, and numerical values are recorded according to an automatic control screen display system;
(6) the system automatically opens a valve of the separating funnel, the methanol flows into the extraction cup, the methanol is evaporated, the methanol flows into the reagent recovery device through the conduit, and the wastewater and the residues are discharged into a waste collection bag for centralized treatment;
(7) and (3) obtaining the fat content according to the fat mass measured in the step (5) and the biological tissue weight measured in the step (1), and displaying the calculation result on an automatic control screen display system.
Preferably, in the above method for measuring the fat content in aquatic organisms, the step (3) specifically comprises:
A. setting the volumes of chloroform and methanol added for the first time in the system as 5/4 water content and 5/2 water content respectively, and setting the homogenizing time as 2 min;
B. setting the volume of chloroform as solvent for the second time of the system as 5/4 water content and setting the homogenization time as 30 s;
C. the volume of solvent water added for the third time of the system was set at 5/4 moisture content and the homogenization time was set at 30 s. The beneficial effects of the above technical scheme are: chloroform is used for dissolving lipid components, methanol can crack lipid bound with protein, and after water is added, small molecular substances such as polysaccharide, amino acid and the like in a sample are dissolved in a methanol-water phase. The first addition of methanol cleaves the protein-bound lipids in the sample, and chloroform dissolves the lipid components, since chloroform is denser and immiscible than methanol, with chloroform in the lower layer and methanol in the upper layer. Adding chloroform for the second time can extract lipid in the sample again; and water is added for the third time to dissolve small molecular substances such as polysaccharide, amino acid and the like in the sample, and because the water is mutually soluble with the methanol and is not soluble with the chloroform, the water and the methanol are on the upper layer, and the chloroform is on the lower layer.
Preferably, in one of the above methods for testing the fat content in aquatic organisms, the chloroform rinsing agitator and the residue flowing out of the wall of the homogenizer in a volume of 15/8, whose water content is a water content, after the filtration in the step (4).
The beneficial effects of the above technical scheme are: on the one hand, the method aims to extract fat to the maximum extent and reduce the residue of the fat on the wall of the container and residues. On the other hand, the reagent is saved. Through multiple experiments, the residual fat recovered at this ratio was found to be approximately 6% of the total extracted fat.
According to the technical scheme, compared with the prior art, the utility model discloses a device of full-automatic test aquatic organism internal fat content changes expensive instrument and equipment expense in the past and consuming time in the past, loaded down with trivial details fat extraction method, provides technical support for confirming aquatic organism internal fat content.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of a frame structure of the device of the present invention;
FIG. 2 is a schematic diagram of the overall structure of the device of the present invention;
FIG. 3 is a schematic view of the bottom of the homogenizer according to the present invention;
fig. 4 is a schematic view of the process flow of the device of the present invention.
In the figure:
1 is a motor, 2 is a stirring cutter head, 3 is a homogenizer, 4 is a pipeline, 5 is a reagent storage device, 6 is a probe, 7 is an automatic control screen display system, 8 is a porous funnel, 9 is a separating funnel, 10 is a pump, 11 is a glass piston, 12 is a detection rod, 13 is an extraction cup, 14 is a reagent recovery device, 15 is a condensing device, 16 is a weighing scale and 17 is a heating device.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it should be understood that the described embodiments are only some embodiments of the present invention, but not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a device of fat content in test aquatic organism, the full-automatic fat that draws of mainly used to reach the fat content that contains in each organism, can realize the automatic purpose of calculating out the organism fat content of surveying.
The device for testing the fat content in the aquatic organisms provided by the embodiment comprises a tissue homogenate and reagent conveying system, a filtering and extracting system, a heating and reagent recovery system, a weighing system and an automatic control screen display system.
The tissue homogenate and reagent conveying system firstly measures the moisture content of an organism through an induction type moisture tester arranged in the tissue homogenate and reagent conveying system, calculates the amount of a required injected solvent according to the proportion of moisture and each solvent, conveys the reagent into a homogenizer quantitatively through a pipeline, and a stirring tool bit homogenizes the organism according to set time;
the filtering and extracting system firstly filters the homogenized organisms according to filtering layers with different pore diameters, and then separates two immiscible liquids formed after filtering in a separating funnel;
the heating and reagent recovery system is used for evaporating the solvent left from the separating funnel according to heating so as to evaporate the solvent to extract fat, and recovering and recycling the reagent through a recovery pipeline;
the weighing system is used for weighing organisms before tissue homogenization and weighing fat after heating evaporation;
and automatically controlling screen display system equipment to set parameters required by the system according to a touch screen and setting a specific operation flow of an experiment.
The embodiment of the utility model provides a device of fat content in test aquatic organism specifically as follows:
1. tissue homogenate and reagent delivery system
The system mainly comprises a motor 1, a stirring cutter head 2, a refiner 3, an induction type moisture tester, a pipeline 4 with a spray head and a reagent storage device 5. A cylindrical motor 1 with the height of 15cm and the radius of 5cm is arranged at the upper part of the stirring device, a refiner 3 with the height of 30cm and the radius of 10cm is fixed on the motor 1, a stirring cutter head 2 capable of setting the rotating speed according to a system is arranged in the middle of the refiner 3, the kinetic energy of the stirring cutter head 2 is provided by the motor 1, and the stirring cutter head 2 is fixed at the upper part of the stirring device to stir aquatic organisms. The probe 6 of the induction type moisture tester is positioned in the middle of the stirring tool bit 2, and can automatically extend to the organism along with the opening of the instrument, detect the moisture of aquatic organism tissues and display the moisture on the automatic control screen display system 7. The spray head is positioned in the stainless steel homogenizer 3 and is 5cm away from the top of the homogenizer 3, and according to the set liquid volume, the spray head can spray corresponding solvent amount into the homogenizer 3 according to the set experimental solvent adding proportion, so that the four walls and the bottom of the stainless steel homogenizer 3 are washed. Three guide pipes are connected to the spray head, valves of the corresponding guide pipes are opened according to reagent requirements, the total length of each pipe is 1 meter, and the reagents are placed in 3 4L glass bottles and respectively filled with distilled water, chloroform and methanol. The bottom of the homogenizer 3 is composed of two stainless steel semicircles, after homogenization, the two semicircles are separated towards two sides, organisms and liquid can flow into a funnel of the bottom filtering and extracting system, and the two semicircles are closed immediately.
2. Filtration and extraction system
The system mainly comprises a ceramic porous funnel 8, a separating funnel 9 and a pump 10. At the bottom of the homogenizer 3 is a ceramic porous funnel 8, the inner diameter of the porous funnel 8 is 20cm, whatman No.1 filter paper is automatically changed by a grip handle, the homogenate flows through the funnel, and the funnel sucks chloroform and methanol into a lower separatory funnel 9 by means of suction of a pump. Chloroform methanol is divided into two layers by the pear-shaped glass separating funnel 9, the glass piston 11 can control the switch of the separating funnel 9 according to a sensor, the upper part of the glass piston 11 is provided with a detection rod 12 which can detect the conversion of two liquids, when the lower liquid flows into the extraction cup 13 of the heating and reagent recovery system and completely flows out, and methanol liquid appears, the glass piston 11 is automatically closed.
3. Heating and reagent recovery system
The system consists of an extraction cup 13, a reagent recovery device 14, a condensing device 15 and a heating device 17. Liquid flows into a lower extraction cup 13 through a separating funnel 9, the heating device 17 can evaporate a liquid solvent into a reagent recovery device 14 for storing chloroform through the set experimental heating temperature, when no chloroform flows in a conduit, the system can convey the extraction cup 13 into a weighing system 16 for weighing, the mass of fat is automatically recorded, the weight is displayed in an automatic control screen display system 7, then a glass piston 11 is automatically opened to release methanol, and the liquid is continuously evaporated into the reagent recovery device 14 for storing methanol through the set experimental heating temperature. The left upper part of the extraction cup 13 is provided with a pipeline with a valve, evaporated solvent gas is condensed by a condensing device 15 through the pipeline and then is converted into liquid to flow into a reagent recovery device 14 at the bottom of the pipeline, the used solvent is recovered, and the recovered reagent can be conveyed back to the reagent storage device 5 for storing the solvent through the pipeline for recycling.
4. Weighing system
The bottom of the system is provided with a weighing meter 16, the aquatic organism tissue is placed in the weighing system for weighing before being placed in the system, at the moment, the automatic control screen display system 7 automatically counts the mass of the organism, and then the system conveys the aquatic organism tissue into a homogenizer 3 of a tissue homogenizing and reagent conveying system. When the chloroform in the extraction cup 13 is completely evaporated, the system will send the dried fat to the weighing meter 16 for weighing, and display the specific value after the fat quality of the system 7 is automatically controlled and displayed.
5. Automatic control screen display system equipment
The whole experimental process can be controlled according to the automatic control screen display system 7, and the solvent ratio added in the experiment, the experimental time of each step, the experimental heating temperature, the display of the fat quality and the calculation of the fat content can be set in the automatic control screen display system 7. The automatic control screen display system 7 is 30cm long, 20cm wide and 8cm high, and can be set through a touch screen.
The method for testing the fat content in the aquatic organisms by the device comprises the following steps:
the fish body with the removed fish scales, the removed fish heads and the measured weight is put into a weighing system, the system automatically weighs the fish body, the specific numerical value of the weight of the organism is displayed in an automatic control screen display system, and then the system sends the fish body into a stirrer;
setting parameters according to an automatic control screen display system, opening an induction type moisture meter switch in the automatic control screen display system to measure the water content of the fish body, automatically calculating the water content of the fish body by the system according to the weight and the water content of the organism, automatically setting the volumes of chloroform and methanol added for the first time of the system to be 5/4 water content and 5/2 water content respectively (if the water content is 80ml, the volumes of the chloroform and the methanol added for the first time are respectively calculated to be 100ml and 200ml according to the proportion), and automatically setting the homogenate time to be 2 min; automatically setting the volume of chloroform as solvent for the second time to 5/4 water content (for example, if the water content has been calculated to be 80ml, then the required amount of chloroform is automatically calculated to be 100ml according to the proportion), and automatically setting the homogenization time to 30 s; the volume of solvent water added for the third time in the system was automatically set to 5/4 water content (e.g., if the system had calculated a water content of 80ml, then the proportion automatically calculated to require 100ml of water to be added), and the homogenization time was set to 30 s. When other non-recommended solvents are used, the proportion of each solvent and the homogenization time can be manually set through a control panel on the automatic control screen display system;
the homogenized material was passed from the bottom of the homogenizer to a coors No.3 porous funnel with whatman No.1 filter paper, and was suction filtered by a pump, the system was set to flow 15/8 with the amount of chloroform as water content from the homogenizer walls after filtration (the system automatically calculated the volume of chloroform based on the water content, e.g., 150ml for 80 ml), the homogenizer walls were rinsed and then passed out of the funnel to rinse the stirrer and residue;
the liquid flowing out of the porous funnel directly flows into a separating funnel at the lower part, the static time of the liquid flowing into the separating funnel through an automatic control screen display system is 2 minutes, the lower chloroform layer flows into an extraction cup, the chloroform is evaporated at the first heating temperature set by the automatic control screen display system, the chloroform gas firstly flows into a device for recovering the chloroform through a conduit, when the system detects that no chloroform flows into the conduit, the system can send the extraction cup into a weighing system for weighing, and the specific numerical value is displayed after the fat quality of the automatic control screen display system. And then, the system automatically opens a valve of the separating funnel, the methanol flows into the extraction cup, the second heating temperature is set according to the automatic control screen display system to evaporate the methanol, and the methanol flows back to the methanol recovery reagent recovery device through the guide pipe. The solvent in the recovery device is conveyed back to the corresponding storage device through a pipeline for reuse;
according to the calculation method set in the system, namely dividing the fat mass measured by the equipment by the weight of the organism to obtain the fat content, automatically calculating the fat content on the automatic control screen display system, and displaying the calculation result.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. An apparatus for measuring the fat content in an aquatic organism, comprising
A tissue homogenization and reagent delivery system comprising a reagent storage device, a homogenizer, and an induction moisture meter, the reagent storage device being in communication with the homogenizer via a conduit, and the induction moisture meter being located within the homogenizer;
the filtering and extracting system comprises a porous funnel and a separating funnel, the porous funnel is positioned at the bottom of the homogenizer, and the separating funnel is connected with a liquid outlet of the porous funnel;
the heating and reagent recovery system comprises an extraction cup, a reagent recovery device, a heating device and a condensing device, wherein the extraction cup is positioned at the liquid outlet of the separating funnel, the extraction cup is connected with the reagent recovery device through a pipeline, and the reagent recovery device is communicated with the reagent storage device;
a weighing system for weighing the organisms and the extraction cup;
and the automatic control screen display system is used for setting parameters and specific operation processes required by the whole system and receiving and displaying the measurement results.
2. The apparatus of claim 1, wherein the reagent storage means comprises three storage bottles for storing distilled water, chloroform and methanol, respectively, and the three storage bottles are connected to the homogenizer through pipes.
3. An apparatus as claimed in claim 1 or 2, wherein the conduit is provided with a spray head at one end of the homogenizer.
4. The apparatus of claim 1, wherein a stirring blade is disposed in the homogenizer.
5. The apparatus of claim 1, wherein the bottom of the homogenizer comprises two stainless steel semicircles.
6. The apparatus of claim 1, wherein a sensor for controlling the opening and closing of the separating funnel and a detection probe for detecting the liquid change are disposed at the glass piston of the separating funnel.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201922075725.8U CN211292434U (en) | 2019-11-26 | 2019-11-26 | Device for testing fat content in aquatic organism |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201922075725.8U CN211292434U (en) | 2019-11-26 | 2019-11-26 | Device for testing fat content in aquatic organism |
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| CN211292434U true CN211292434U (en) | 2020-08-18 |
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| CN201922075725.8U Active CN211292434U (en) | 2019-11-26 | 2019-11-26 | Device for testing fat content in aquatic organism |
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