CN106198111A - A kind of on-line sample acquisition and storage device - Google Patents
A kind of on-line sample acquisition and storage device Download PDFInfo
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- CN106198111A CN106198111A CN201610691575.1A CN201610691575A CN106198111A CN 106198111 A CN106198111 A CN 106198111A CN 201610691575 A CN201610691575 A CN 201610691575A CN 106198111 A CN106198111 A CN 106198111A
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- syringe
- entry needle
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- 238000003860 storage Methods 0.000 title claims abstract description 49
- 239000007788 liquid Substances 0.000 claims abstract description 89
- 238000002347 injection Methods 0.000 claims abstract description 43
- 239000007924 injection Substances 0.000 claims abstract description 43
- 238000005070 sampling Methods 0.000 claims abstract description 39
- 238000004140 cleaning Methods 0.000 claims abstract description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- 238000012544 monitoring process Methods 0.000 claims abstract description 7
- 239000012528 membrane Substances 0.000 claims description 31
- 239000002699 waste material Substances 0.000 claims description 28
- 239000012530 fluid Substances 0.000 claims description 25
- 238000005406 washing Methods 0.000 claims description 23
- 241001411320 Eriogonum inflatum Species 0.000 claims description 13
- 238000000605 extraction Methods 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 239000011148 porous material Substances 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 8
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 244000005700 microbiome Species 0.000 abstract description 8
- 238000012258 culturing Methods 0.000 abstract description 4
- 239000000523 sample Substances 0.000 description 158
- 239000003814 drug Substances 0.000 description 17
- 238000001514 detection method Methods 0.000 description 15
- 229940079593 drug Drugs 0.000 description 14
- 238000002156 mixing Methods 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 9
- 230000002906 microbiologic effect Effects 0.000 description 7
- 239000010413 mother solution Substances 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 229940088515 ciloxan Drugs 0.000 description 3
- 229960003405 ciprofloxacin Drugs 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 239000006101 laboratory sample Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 1
- 230000004308 accommodation Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
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- 238000012549 training Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The open a kind of on-line sample acquisition and storage device of the present invention, relates to microorganism culturing field, including sample collecting apparatus, sample storage device, cleans device, mechanical injection device and control device；Sample collecting apparatus is connected with mechanical injection device, and the sample liquid extracted in pipeline is injected in mechanical injection device；Clean device the entry needle in mechanical injection device to be carried out disinfection cleaning；Sample liquid is injected in sample storage device by mechanical injection device；Described control device and harvester, sample storage device, clean device, mechanical injection device is connected.The present invention is capable of automatic sampling, storage and cleaning operation, it is to avoid efficiency that manual operation causes, safety, monitoring verity low, and this device is simple to operate, and efficiency is higher, and safety is the highest.
Description
Technical field
The present invention relates to microorganism culturing field, particularly relate to a kind of on-line sample acquisition and storage device.
Background technology
Existing microorganism culturing instrument needs to gather sample to detect the growing state of microorganism.Side of the prior art
Formula uses manual sampling mostly, and workload is big, and engineering is loaded down with trivial details, and cell sample easily pollutes, and can not monitor in real time.Traditional is micro-
Biological sampling mode is that artificial sampling platform of being put into by culture dish carries out after sampling completes, manually being taken out by culture dish, then sending
Entering incubator to cultivate, this process is complicated, discontinuous, needs personnel to get involved, and efficiency and safety are low, and the verity monitored
Also reduce.
And, mix the semisolid of liquefaction and the micro-biological samples that viscosity is high by hand, take long, efficiency is low, work into
This height, operator closely terminate there is infection risk with the sample with pathogenic bacterium, meanwhile, manual withdrawal various microorganism sample
Product, are easily affected by anthropic factor and environmental factors, and random and nonstandardized technique operation can affect the culture effect of microbionation,
Irreversible malpractice can be caused.
At present, existing automatization microbiological specimens processing equipment, there is mixing liquefaction weak effect, the time is long, separates effect
The most undesirable, many defects such as structure design is unreasonable, it is impossible to meet microorganism field to microbiological specimens automatic business processing
Demand.Existing microbe sampling instrument kind is the most, carries out, from moving ware (many wares), more not adopting when also cannot sample
Sampling instrument, storage apparatus and cleaning instrument are combined as a whole.
Number of patent application is " the laboratory sample collecting apparatus " of 201220653639.6, discloses medical science chemical examination auxiliary and sets
A kind of laboratory sample collecting apparatus in standby field, the M type acquisition component corresponding by pair of openings is extended by rotating shaft
Go out to accommodate hand pump, this laboratory sample collecting apparatus simple in construction, simple to operate, safety, and energy in gathering sample
Enough microorganism is effectively protected so it does not leak out.But its using method is manually to operate, it is impossible to realize
The automatic acquisition operations of microbiological incubator sample.
Number of patent application is " a kind of sample collecting apparatus " of 201120292650.X, comprises collection body, covers base, go out
Lid, this sampling body includes: hollow body；Entrance, outlet, accommodation space；This capping can coordinate this entrance to be assembled in
One section of hollow body；And this outlet cap, this outlet can be coordinated to be assembled in one end of hollow body.Make preservation sample and taking-up
Sample is incorporated into same device, and allow inspection carry out convenient.But its using method is easily manual operation, nothing
Method realizes the automatic collection of sample.
Summary of the invention
For the problems referred to above, the present invention proposes a kind of on-line sample acquisition and storage device for microbiological incubator, should
Device is simple to operate, and efficiency is higher, and safety is the highest, it is possible to increase the Blending Efficiency of Blending of microbial culture medium.
Technical scheme
A kind of on-line sample acquisition and storage device, including sample collecting apparatus, sample storage device, cleans device, mechanical injection
Device and control device；Sample collecting apparatus is connected with mechanical injection device, and the sample liquid extracted in pipeline is injected into machine
In tool injection device；Clean device the entry needle in mechanical injection device to be carried out disinfection cleaning；Mechanical injection device is by sample
Liquid injects in sample storage device；Described control device and harvester, sample storage device, cleans device, mechanical injection
Device is connected.
Further technical scheme, described mechanical injection device includes mechanical arm, pin folder and entry needle；Described machinery
Arm is connected with entry needle by pin folder, and described mechanical arm controls entry needle motion in space, described mechanical arm and in
Control device is connected.Mechanical arm is capable of entry needle, every bottle stopper plate, the alignment of sample pore；When entry needle moves downward, alignment
Insert sample cell aperture；After entry needle injects sample cell, move to entry needle Washing cup cleans.
Further technical scheme, described sample collecting apparatus includes syringe, sampling needle, fixed plate, push-and-pull slide block, V
Type block, depression bar molectron, line slideway, lead screw motor, feed screw nut's connecting plate, optocoupler catch, optocoupler, tee T；Described
The piston handle of syringe is fixed on line slideway by push-and-pull slide block, and line slideway is fixed on the top of fixed plate；
Described optocoupler catch is arranged on feed screw nut's connecting plate, and optocoupler is arranged on the middle part of fixed plate；Control device passes through
Judge the reset of optocoupler signal control motor, it is to avoid the piston of syringe is pressed onto bottom.
The staving of described syringe is fixed on the bottom of fixed plate by depression bar molectron, V-block；
Described lead screw motor is connected with push-and-pull slide block by feed screw nut's connecting plate；Push-and-pull slide block can be at the band of lead screw motor
Move up and down at slide rail under Dong, and then drive pull-up and the depression of syringe.
Described lead screw motor is connected with control device.
Described syringe is connected with the entry needle of sampling needle, mechanical injection device by tee T.
Further technical scheme, described tee T is Y-shaped three-way pipe, described tee T and sampling needle, machinery note
The pipeline that the entry needle of injection device connects is provided with pinch valve；Pinch valve has two, the most normally opened pinch valve and the normally off clamp
Valve.When syringe pulls up, normally opened pinch valve is opened, and normally closed pinch valve closes, and completes the extraction of sample liquid；When pressing under syringe,
Normally opened pinch valve closes, and normally closed pinch valve is opened, and completes sample liquid and is injected into sample storage device.
Further technical scheme, described tee T fixed by pinch valve, and pinch valve is fixed on pipe clamp.
Further technical scheme, described sample storage device include reducing motor, bearing block, rotation dish, sample box, every
Bottle stopper plate；Described sample box is located on rotation dish；Described reducing motor is connected with sample box support；Described bearing block is solid
Being scheduled on sample box support, described rotation dish is connected with the screw mandrel of reducing motor, and described is fixed on sample box every bottle stopper plate
On support, being positioned at the surface at sample box center, described reducing motor is connected with control device.When entry needle is from every bottle stopper
When the downward fluid injection of plate is to sample cell, sample cell pipe cap can be avoided to come off with the rising of entry needle.
Further technical scheme, described sample box is provided with sample pore, is used for placing sample cell.Sample cell is placed on
On sample pore, wait the injection of entry needle.
Further technical scheme, also includes Hall element, described hall sensing in described sample storage device
The bottom of sample box is located at by device, is fixed on the position that sample box mid-stent is on the lower side, and the bottom of sample box is fixed with fritter
Magnet, when sample collecting apparatus electrification reset, control device judges the position of Magnet by Hall element, completes sample box
The reset of original position.
Further technical scheme, described cleaning device includes Washing cup, membrane pump, level sensor, connects liquid bottle, waste liquid
Bottle；Described membrane pump includes that the first membrane pump and the second membrane pump, the first described membrane pump extraction connect the cleaning in liquid bottle
Liquid, in the described waste liquid in the second membrane pump extraction Washing cup to waste liquid bottle.Described level sensor is located at and is connect liquid bottle one
Side, connects the cleanout fluid surplus in liquid bottle for monitoring, and the first described membrane pump and the second membrane pump are all connected with control device.
While entry needle moves into Washing cup, the first membrane pump work so that cleanout fluid rinses entry needle.Meanwhile, the second membrane pump
Work, the waste liquid in extraction Washing cup enters in waste liquid bottle.When the cleanout fluid deficiency connect in liquid bottle, level sensor can
Monitoring the remaining cleaning fluid volume of cleanout fluid and be uploaded to control device send warning operation.
Further technical scheme, described Washing cup electroplate sheath.
Further technical scheme, described on-line sample harvester also includes ultraviolet sterilization device, described ultraviolet
Sterilizing unit is located at the side of entry needle.
Further technical scheme, described sample cell is vacuum test tube.
Further technical scheme, a kind of on-line sample acquisition and storage device, the user of described sample collecting apparatus
Method is:
Step one: start syringe by the sample liquid in sampling needle collect specimen to syringe, then by the sample in syringe
Product liquid is expelled in specimen, repeat the above steps；
Step 2: pushing syringe, the waste liquid in the pipeline be connected syringe with entry needle is discharged；Described waste liquid is discharged can
To be expelled in Washing cup, use cleanout fluid that entry needle syringe needle is carried out simultaneously；
Step 3: after waste liquid is completely exhausted out, is injected to entry needle by sample liquid, collects sample liquid.
Further technical scheme, the number of repetition of described step one is at least one times.
Further technical scheme, further technical scheme, the amount of the described sample liquid in syringe is more than being wanted
Collect the amount of sample liquid.
When the circulation fluid path of microorganism culturing works, control device sends sample collecting and instructs to sample collecting apparatus,
Wherein syringe makes pressure twice i.e. pump drainage twice of leaving behind by oneself, carries out the microbial culture medium cultivated in pipeline mixed of incubator
Even, then carry out sample collecting.Per injection device pump drainage markers is originally by gathering in pin entrance syringe, during pressure in syringe
Sample liquid is pushed in specimen by gathering pin, confuses with specimen, then proceedes to take out specimen with syringe, then press, at least twice
After taking out pressure, not having the sample liquid residual of last collection in syringe, the sample liquid collected is this sample gathered
Liquid.
General once sampling needs two sample cells to collect sample, operates to realize the pre-discharge opeing of syringe, sample liquid
Extraction amount need the quantitative values more than two sample cells.After sample liquid enters entry needle, entry needle elder generation removal waste fluid, main mesh
The waste liquid remained in when being to sample the last time in entry needle discharge fall.Waste liquid is discharged in Washing cup by entry needle, makes
With cleanout fluid, entry needle is carried out.After the cleaning of cleanout fluid, move entry needle by mechanical arm, then by the electricity that slows down
Machine rotates specimen disc, it is achieved entry needle and new sample cell, being directed at every bottle plate washer aperture, and syringe is pressed still further below, completes note
Sample liquid in emitter enters the injection in sample cell and storage operation.
Sample liquids after collection is saved in the sample storage environment of 4 degrees Celsius.Can be used for the counting inspection of cell, antibacterial liquid
Survey or be used for the detection of liquor strength.
Beneficial effect:
1, a kind of on-line sample acquisition and storage device of the present invention is capable of automatic sampling, storage and cleaning operation, it is to avoid
Efficiency that manual operation causes, safety, monitoring verity low, and this device is simple to operate, and efficiency is higher, and safety is the most relatively
High.
2, Washing cup surface of the present invention is provided with silver-plated sheath, it is possible to reduce germ contamination, keeps the nothing of Washing cup self
Collarium border, and silver-plated with low cost, effective time is long, promotes the cleaning performance of entry needle.
3, the mechanical arm of the present invention is capable of entry needle, every bottle stopper plate, the alignment of sample pore；Entry needle moves downward
Time, alignment inserts sample cell aperture；After entry needle injects sample cell, move to entry needle Washing cup cleans, it is possible to
Realize automatic sample, and be automatically directed at sample cell.
4, the control device of the present invention reset by optocoupler signal control motor, it is to avoid the piston of syringe is pressed onto the end
Portion.
5, automatization's sample collecting apparatus of the present invention reduces production cost, and eliminates the height that labyrinth brings
The problem of fault rate.
6, the present invention can separate vacuum specimen tube and entry needle every bottle stopper plate, prevent subnormal ambient hemostasis to be needled into
Frictional force is relatively big after vacuum specimen tube and between rubber surface plug, pull-up vacuum specimen tube.
7, the Hall element of the present invention has the feature of stable performance, accurate positioning, for bottom with little magnetic piece
The initial reset of sample box.Especially in the case of 4 degrees Celsius of sample preserves, condensed water, Hall element phase can be produced
The original papers such as contrast infrared sensor have the characteristic not affected by condensed water, it is possible to stably play a role.
8, the membrane pump in the present invention can be in severe working environment, such as during discharge of wastewater, due in sewage
Impurity is many and complicated, and pipeline is prone to blocking, and electric pump so just produces the situation that load is too high, and the motor feels hot and rapid wear
Consumption, and pneumatic diaphragm pump can be adjustable by granule and flow, is automatically stopped to unobstructed during line clogging.Level sensor can be real
Executing monitoring, prevent cleanout fluid to lack and cause cleaning unsuccessfully, the situation of sample contamination occurs.And can automatically monitor, reduce people
Work checks cost and region internal contamination probability.
9, the ultraviolet-sterilization of the present invention is different from other sterilization modes, has good sterilizing function, and safety to air
Pollution-free, it is possible to control at any time, it is arranged on entry needle side it can be avoided that the sample in glass tube with vacuum is caused damage.
10, inventive samples pipe uses vacuum specimen tube, it is possible to avoids polluting, and is enclosed environment, more conventional sampling tube,
Vacuum specimen tube need not manually close the lid, and is prevented effectively from pollution, keeps sample data verity.
11, the present invention is during gathering sample liquid, and the sample liquid that can remain in last time in syringe is carried out at least
Twice pump drainage, can be discharged into the waste liquid remained in pipeline in culture vessel, the syringe after pump drainage through twice in advance
The sample liquid being finally extracted into is consistent with specimen.One section of liquid is first discharged discharging the when that sample liquid entering sample cell,
Then entry needle is cleaned, it is possible to remove the probability of sample carryover last time.Through this step, detection can be significantly improved accurately
Rate, and without changing syringe, reduce equipment cost.Advantageously ensure that the sample liquid of collection does not has sample last time
Residual, and then improve experiment Detection accuracy.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention
The front view of sample collecting apparatus in Fig. 2 present invention；
Fig. 3 is the rearview of sample collecting apparatus in the present invention；
Fig. 4 is sample storage device in the present invention, cleans the structural representation of device；
Fig. 5 is embodiment of the present invention Chinese medicine standard curve；
Labelling in figure: 1, sample collecting apparatus, 2, sample storage device, 3, clean device, 4, mechanical injection device, 5, middle control dress
Put, 6, ultraviolet sterilization device；
1-1, syringe, 1-2, sampling needle, 1-3, fixed plate, 1-4, push-and-pull slide block, 1-5, V-block, 1-6, depression bar molectron, 1-
7, line slideway, 1-8, lead screw motor, 1-9, feed screw nut's connecting plate, 1-10, optocoupler catch, 1-11, optocoupler, 1-12, threeway
Pipe, 1-13, pinch valve, 1-14, pipe clamp；1-15, injection pipeline；1-16 sample lines；1-17 culture fluid pipeline；
2-1, reducing motor, 2-2, bearing block, 2-3, rotation dish, 2-4, sample box, 2-5, sample box support, 2-6, every bottle stopper
Plate, 2-7, Hall element, 2-8, sample cell；
3-1, Washing cup, 3-2, the first membrane pump, 3-3, the second membrane pump, 3-4, level sensor, 3-5, connect liquid bottle, 3-6,
Waste liquid bottle；
4-1, mechanical arm, 4-2, pin folder, 4-3, entry needle.
Detailed description of the invention
The present invention is further detailed explanation with embodiment below in conjunction with the accompanying drawings.
Embodiment
As shown in Figures 1 to 4, a kind of on-line sample acquisition and storage device, including sample collecting apparatus 1, sample storage device
2, clean device 3, mechanical injection device 4 and control device 5；Sample collecting apparatus 1 is connected with mechanical injection device 4, and will pipe
In road, the sample liquid of extraction is injected in mechanical injection device 4；Clean device 3 the entry needle 4-3 in mechanical injection device 4 is entered
Row sterilization is cleaned；Sample liquid is injected in sample storage device 2 by mechanical injection device 4；Described control device 5 and harvester
1, sample storage device 2, clean device 3, mechanical injection device 4 is connected.
Described mechanical injection device 4 includes that mechanical arm 4-1, pin press from both sides 4-2 and entry needle 4-3；Described mechanical arm 4-1 leads to
Crossing pin folder 4-2 to be connected with entry needle 4-3, described mechanical arm 4-1 controls entry needle 4-3 motion in space, described machine
Mechanical arm 4-1 is connected with control device 5.Mechanical arm 4-1 is capable of entry needle 4-1, every bottle stopper plate 2-6, the alignment of sample pore；
When entry needle 4-3 moves downward, alignment inserts sample cell aperture；After entry needle 4-3 injects sample cell 2-8, by entry needle
4-3 moves to clean in Washing cup 3-1.
Described sample collecting apparatus 1 includes syringe 1-1, sampling needle 1-2, fixed plate 1-3, push-and-pull slide block 1-4, V-block
1-5, depression bar molectron 1-6, line slideway 1-7, lead screw motor 1-8, feed screw nut connecting plate 1-9, optocoupler catch 1-10, optocoupler
1-11, tee T 1-12；The piston handle of described syringe 1-1 is fixed on line slideway 1-7 by push-and-pull slide block 1-4,
Line slideway 1-7 is fixed on the top of fixed plate 1-3；Described optocoupler catch 1-10 is arranged on feed screw nut connecting plate 1-9,
Optocoupler 1-11 is arranged on the middle part of fixed plate 1-3；Control device 5 is by judging the reset of optocoupler signal control motor, it is to avoid note
The piston of emitter 1-1 is pressed onto bottom.
The staving of described syringe 1-1 is fixed on the bottom of fixed plate 1-3 by depression bar molectron 1-6, V-block 1-5；
Described lead screw motor 1-8 is connected with push-and-pull slide block 1-4 by feed screw nut connecting plate 1-9；Push-and-pull slide block 1-4 can be at screw mandrel
Move up and down at slide rail under the drive of motor 1-8, and then drive pull-up and the depression of syringe 1-1.Described screw mandrel electricity
Machine 1-8 is connected with control device 5.Described syringe 1-1 is by tee T 1-12 and sampling needle 1-2, mechanical injection device 4
Entry needle 4-3 connects.
Described tee T 1-12 is Y-shaped three-way pipe, described tee T and sampling needle 1-2, the note of mechanical injection device 4
The pipeline penetrating pin 4-3 connection is provided with pinch valve 1-13；Pinch valve has two, the most normally opened pinch valve and the normally off pinch valve.
When syringe pulls up, normally opened pinch valve is opened, and normally closed pinch valve closes, and completes the extraction of sample liquid；When pressing under syringe, normally opened
Pinch valve closes, and normally closed pinch valve is opened, and completes sample liquid and is injected into sample storage device.Described tee T 1-12 passes through pinch valve
1-13 fixes, and pinch valve 1-13 is fixed on pipe clamp.
Described sample storage device 2 includes reducing motor 2-1, bearing block 2-2, rotation dish 2-3, sample box 2-4, sample box
Support 2-5, every bottle stopper plate 2-6；Described sample box 2-4 is located on rotation dish 2-3；Described reducing motor 2-1 and sample box
Support 2-5 connects；Described bearing block 2-2 is fixed on sample box support 2-5, described rotation dish 2-3 and reducing motor 2-1
Screw mandrel connect, described is fixed on sample box support 2-5 every bottle stopper plate 2-6, is positioned at the surface at sample box 2-4 center,
Described reducing motor 2-1 is connected with control device 5.When entry needle 4-3 is from every the bottle stopper downward fluid injection of plate 2-6 to sample cell 2-
When 8, sample cell pipe cap can be avoided to come off with the rising of entry needle.Described sample box 2-4 is provided with sample pore, is used for putting
Put sample cell 2-8.Sample cell 2-8 is placed on sample pore, waits the injection of entry needle 4-3.
Described sample storage device 2 also including, Hall element 2-7, described Hall element 2-7 are located at sample
The bottom of box 2-4, is fixed on position on the lower side in the middle part of sample box support 2-5, and the bottom of sample box 2-4 is fixed with fritter magnetic
Ferrum, when sample collecting apparatus 1 electrification reset, control device 5 judges the position of Magnet by Hall element 2-7, completes sample
The reset of product box 2-4 original position.
Described cleaning device 3 includes Washing cup 3-1, membrane pump, level sensor 3-4, meets liquid bottle 3-5, waste liquid bottle 3-6；
Described membrane pump includes that the first membrane pump 3-2 and the second membrane pump 3-3, the first described membrane pump 3-2 extraction meet liquid bottle 3-5
In cleanout fluid, in the waste liquid in described second membrane pump 3-3 extraction Washing cup 3-1 to waste liquid bottle 3-6.Described liquid level sense
Answer device 3-4 to be located at and connect liquid bottle 3-5 side, connect the cleanout fluid surplus in liquid bottle for monitoring, the first described membrane pump 3-2 and the
Two membrane pump 3-3 are all connected with control device 5.While entry needle 4-3 moves into Washing cup 3-1, the first membrane pump 3-2 work
Make so that cleanout fluid rinses entry needle 4-3.Meanwhile, the second membrane pump 3-3 work, the waste liquid in extraction Washing cup 3-1 enters useless
In liquid bottle 3-6.When the cleanout fluid deficiency connect in liquid bottle 3-5, level sensor 3-4 can monitor the remaining cleanout fluid of cleanout fluid
Measure and be uploaded to control device 5 and send warning operation.Described Washing cup 3-1 electroplate sheath.
Described on-line sample harvester 1 also includes that ultraviolet sterilization device 6, described ultraviolet sterilization device are located at injection
The side of pin 4-3.
Described sample cell is vacuum test tube.
A kind of on-line sample acquisition and storage device, the using method of described sample collecting apparatus is:
Step one: by the sample liquid in sampling needle collect specimen to syringe, then the sample liquid in syringe is expelled to
In specimen, it is repeated once above-mentioned steps；
Step 2: pushing syringe, the waste liquid in the pipeline be connected syringe with entry needle is discharged；Described waste liquid is discharged can
To be expelled in Washing cup, use cleanout fluid that entry needle syringe needle is carried out simultaneously；
Step 3: after waste liquid is completely exhausted out, is injected to entry needle by sample liquid, collects sample liquid.
A kind of verification experimental verification of the pull effect of on-line sample harvester.
Test prepares:
1, Ciloxan (mother solution) 1000.5 mg/L of high concentration is configured.
2, the pure water of about 4L is prepared, with the dilution of the hydrochloric environment sand star solution in pipeline to be recycled.
Process of the test:
1) build the fluid path of circulation, utilize culture medium bag as power, to form closed circuit by pipeline as container, pump.Training
Support base bag to be positioned on wobbler, accelerate mixing.In culture medium bag, inject the pure water of 1L, wait 10min so that closed circuit is steady
Fixed.Sampling needle is positioned at pipeline.
Mix the time needing 10 minutes at 1L sample completely, be loaded latter 20 minutes and be sampled detection.
Starting the clock after fluid path stable circulation, initial time is 0, sampling detection.
2) in order to reach drug level 8.06mg/L, running latter 10 minutes injection mother solution amounts is 8.06 ml；Sampling in 30 minutes
Detection
3) in order to reach drug level 11.49mg/L, run continuation in latter 40 minutes injecting mother solution amount is 3.43ml.Take when 60 minutes
Sample detects；
4) in order to reach drug level 15.96mg/L, run continuation in latter 70 minutes injecting mother solution amount is 4.47ml.90 minutes points
Sampling detection during clock；
5) in order to reach drug level 11.29mg/L, run latter 100 minutes and first discharge mixed liquor 293ml, continue to inject pure water
293ml.Sampling detection when 120 minutes minutes；
6) in order to reach drug level 7.2mg/L, run latter 130 minutes and first discharge mixed liquor 362ml, continue to inject pure water
362ml.To be diluted.Sampling detection when 150 minutes minutes；
7) in order to reach drug level 3.95mg/L, run latter 160 minutes and first discharge mixed liquor 452ml, continue to inject pure water
452ml, to be diluted.Sampling detection when 180 minutes minutes；
8) in order to reach drug level 2.51mg/L, run latter 190 minutes and first discharge mixed liquor 364ml, continue to inject pure water
364ml, sampling detection during to be diluted 210 minutes minutes；
9) in order to reach drug level 1.65mg/L, run latter 220 minutes and first discharge mixed liquor 342ml, continue to inject pure water
342ml.Sampling detection during to be diluted 240 minutes minutes；
Sample collecting:
In each moment point, use our on-line sample harvester to carry out direct drawing liquid sampling and (do not mix, the most pre-waste discharge
Liquid) and mixing drawing liquid sampling (2 mixings of pull, pre-removal waste fluid).Then after using 723N visible spectrophotometer to sample
The mensuration of liquor strength, the absorbance of test sample sample.By calculating the extinction of comparative drug (ciprofloxacin)
Angle value, detects the concentration of comparative drug.
During experiment, detection uses spectrophotometer to start shooting before detecting and preheats 20-30 min, then by wavelength
It is transferred to 320 nm.Return to zero with deionized water for blank.The variable concentrations of the mother solution of detection Ciloxan
The absorbance of diluent, duplicate detection twice.
Obtain the standard curve of the absorbance drug level corresponding with ciprofloxacin.
After direct sampling sampling and mixing drawing liquid sampling, detect solution in each sampling tube by the number order of vacuum test tube
Absorbance, then according to " pharmaceutical standards curve ", removes the drug level of the anti-sample solution releasing ciprofloxacin.
Experimental result:
Through actual testing inspection, do not applied two kinds of microbiological specimens systems of pull mixing and application pull mixing
The comparing result of the drug level of Ciloxan, as described in following table one.
The mixing of table 1 not pull and two kinds of drug level contrasts of pull mixing
From upper table and Fig. 5 it can be seen that use mixing pull can significantly improve the Blending Efficiency of Blending of medicine, the medicine detected for 2 times
Concentration value is closer to the Configuration Values of actual liquor strength.
Claims (12)
1. an on-line sample acquisition and storage device, it is characterised in that include sample collecting apparatus, sample storage device, clean
Device, mechanical injection device and control device；Sample collecting apparatus is connected with mechanical injection device, and the sample that will extract in pipeline
Product liquid is injected in mechanical injection device；Clean device the entry needle in mechanical injection device to be carried out disinfection cleaning；Machinery note
Sample liquid is injected in sample storage device by injection device；Described control device and harvester, sample storage device, cleans dress
Putting, mechanical injection device is connected.
A kind of on-line sample acquisition and storage device the most according to claim 1, it is characterised in that described mechanical injection dress
Put and include mechanical arm, pin folder and entry needle；Described mechanical arm is connected with entry needle by pin folder, and described mechanical arm controls note
Penetrating pin motion in space, described mechanical arm is connected with control device.
A kind of on-line sample acquisition and storage device the most according to claim 1, it is characterised in that described sample collecting apparatus
Including syringe, sampling needle, fixed plate, push-and-pull slide block, V-block, depression bar molectron, line slideway, lead screw motor, feed screw nut
Connecting plate, optocoupler catch, optocoupler, tee T；
The piston handle of described syringe is fixed on line slideway by push-and-pull slide block, and line slideway is fixed on fixed plate
Top；
Described optocoupler catch is arranged on feed screw nut's connecting plate, and optocoupler is arranged on the middle part of fixed plate；
The staving of described syringe is fixed on the bottom of fixed plate by depression bar molectron, V-block；
Described lead screw motor is connected with push-and-pull slide block by feed screw nut's connecting plate；
Described syringe is connected with the entry needle of sampling needle, mechanical injection device by tee T；
Described lead screw motor is connected with control device.
A kind of on-line sample acquisition and storage device the most according to claim 3, it is characterised in that described tee T is Y
Type tee T, the pipeline that described tee T is connected with the entry needle of sampling needle, mechanical injection device is provided with pinch valve；Described
Tee T fixed by pinch valve, pinch valve is fixed on pipe clamp.
A kind of on-line sample acquisition and storage device the most according to claim 1, it is characterised in that described sample storage device
Including reducing motor, bearing block, rotation dish, sample box, every bottle stopper plate, sample box support；
Described sample box is located on rotation dish；
Described reducing motor is connected with sample box support；
Described bearing block is fixed on sample box support；
Described rotation dish is connected with the screw mandrel of reducing motor；
Described reducing motor is connected with control device；
Described is fixed on sample box support every bottle stopper plate, is positioned at the surface at sample box center.
A kind of on-line sample acquisition and storage device the most according to claim 5, it is characterised in that set on described sample box
There is sample pore, be used for placing sample cell.
A kind of on-line sample acquisition and storage device the most according to claim 5, it is characterised in that described sample storage dress
Also include in putting that Hall element, described Hall element are located at the bottom of sample box, are fixed on sample box mid-stent inclined
Under position, and the bottom of sample box is fixed with fritter Magnet.
A kind of on-line sample acquisition and storage device the most according to claim 1, it is characterised in that described cleaning device includes
Washing cup, membrane pump, level sensor, connect liquid bottle, waste liquid bottle；
Described membrane pump includes that the first membrane pump and the second membrane pump, the first described membrane pump extraction connect the cleaning in liquid bottle
Liquid, in the described waste liquid in the second membrane pump extraction Washing cup to waste liquid bottle；
The first described membrane pump and the second membrane pump are all connected with control device；
Described level sensor is located at and is connect liquid bottle side, connects the cleanout fluid surplus in liquid bottle for monitoring.
A kind of on-line sample acquisition and storage device the most according to claim 8, it is characterised in that described Washing cup surface
Silver-plated sheath.
A kind of on-line sample acquisition and storage device the most according to claim 1, it is characterised in that described on-line sample
Harvester also includes that ultraviolet sterilization device, described ultraviolet sterilization device are located at the side of entry needle.
11. according to a kind of on-line sample acquisition and storage device described in claim 5 or 6, it is characterised in that described sample cell
For vacuum test tube.
12. a kind of on-line sample acquisition and storage devices according to claim 3, it is characterised in that described sample collecting
The using method of device is:
Step one: by the sample liquid in sampling needle collect specimen to syringe, then the sample liquid in syringe is expelled to
In specimen, repeat the above steps；
Step 2: pushing syringe, the waste liquid in the pipeline be connected syringe with entry needle is discharged；Described waste liquid is discharged can
To be expelled in Washing cup, use cleanout fluid that entry needle syringe needle is carried out simultaneously；
Step 3: after waste liquid is completely exhausted out, is injected to entry needle by sample liquid, collects sample liquid.
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| CN106198111B (en) | 2019-07-19 |
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