A kind of on-line sample acquisition and storage device
Technical field
The utility model is related to microorganism culture field, more particularly to a kind of on-line sample acquisition and storage device.
Background technology
Existing microbial cultivation instrument device needs to gather sample to detect the growing state of microorganism.Side of the prior art
Formula adopts manual sampling mostly, and workload is big, and engineering is loaded down with trivial details, and cell sample easily pollutes, and is unable to monitor in real time.Traditional is micro-
Biological sampling mode be artificial culture dish is put into after the completion of sampling platform sampled, manually culture dish is taken out, is then sent
Enter incubator culture, this process is complicated, discontinuous, need personnel to intervene, efficiency and security are low, and the authenticity that monitors
Also reduce.
And, mix the high micro-biological samples of the semisolid and viscosity of liquefaction by hand, take that long, efficiency is low, work into
This height, operating personnel closely terminate have infection risk with the sample with pathogenic bacteria, meanwhile, the various microorganism samples of manual withdrawal
Product, are easily affected by human factor and environmental factor, and random and nonstandardized technique operation can affect the culture effect of microbionation,
Irreversible malpractice can be caused.
At present, existing automation microbiological specimens processing equipment, exist mixing liquefaction effect poor, the time is long, separate effect
Really undesirable, many defects such as structure design is unreasonable, it is impossible to meet microorganism field to microbiological specimens automatic business processing
Demand.Existing microbe sampling instrument species is very more, carries out moving ware when cannot also sample certainly(Many wares), will more not adopt
Sampling instrument, storage apparatus and cleaning instrument are combined as a whole.
Number of patent application is 201220653639.6 " laboratory sample collecting apparatus ", discloses medical science chemical examination auxiliary and sets
A kind of laboratory sample collecting apparatus in standby field, are extended by rotating shaft by the corresponding M type acquisition components of a pair of openings
Go out to accommodate hand pump, this laboratory sample collecting apparatus simple structure is simple to operate, safety, and energy in collection sample
Enough microorganism is effectively protected so as not to leaking out.But its using method is manually to be operated, it is impossible to realize
The automatic data collection operation of microbiological incubator sample.
" a kind of sample collecting apparatus " of the number of patent application for 201120292650.X, comprising collection body, covers base, goes out
Lid, the sampling body include:Hollow body;Entrance, outlet, accommodation space;The capping can coordinate the entrance and be assembled in
One section of hollow body;And the outlet cap, the outlet can be coordinated and be assembled in one end of hollow body.Make preservation sample and taking-up
Sample is able to be incorporated into same device, and allow inspection carry out convenient.But its using method is easily manually to operate, nothing
Method realizes the automatic data collection of sample.
Utility model content
For the problems referred to above, the utility model proposes a kind of on-line sample collection storage dress for microbiological incubator
Put, the device is simple to operate, and efficiency is higher, and security is also higher, it is possible to increase the Blending Efficiency of Blending of microbial culture medium.
Technical scheme
A kind of on-line sample acquisition and storage device, including sample collecting apparatus, sample storage device, cleaning device, machinery
Injection device and control device;Sample collecting apparatus are connected with mechanical injection device, and the sample liquid extracted in pipeline is injected
Into mechanical injection device;Cleaning device to mechanical injection device in injection needle carry out disinfection cleaning;Mechanical injection device will
In sample liquid injection sample storage device;Described control device and harvester, sample storage device, cleaning device, machinery
Injection device is connected.
Further technical scheme, described mechanical injection device include mechanical arm, pin folder and injection needle;Described machinery
Arm is connected with injection needle by pin folder, and described mechanical arm controls motion of the injection needle in space, and described mechanical arm is with
Control device is connected.Mechanical arm can realize injection needle, every bottle stopper plate, the alignment of sample pore;When injection needle is moved downward, alignment
Insertion sample cell aperture;After injection needle injection sample cell, injection needle is moved in Washing cup and is cleaned.
Further technical scheme, the sample collecting apparatus include syringe, sampling needle, fixed plate, push-and-pull slide block, V
Type block, depression bar sub-assembly, line slideway, lead screw motor, feed screw nut's connecting plate, optocoupler catch, optocoupler, three-way pipe;Described
The piston handle of syringe is fixed on line slideway by push-and-pull slide block, and line slideway is fixed on the top of fixed plate;
, on feed screw nut's connecting plate, optocoupler is installed in the middle part of fixed plate for described optocoupler catch;Control device
By judging the reset of optocoupler signal control motor, it is to avoid the piston of syringe is pressed onto bottom.
The staving of described syringe is fixed on the bottom of fixed plate by depression bar sub-assembly, V-block;
Described lead screw motor is connected with push-and-pull slide block by feed screw nut's connecting plate;Push-and-pull slide block can be in lead screw motor
Drive under move up and down in slide rail, and then drive syringe pull-up and depression.
Described lead screw motor is connected with control device.
Described syringe is connected with the injection needle of sampling needle, mechanical injection device by three-way pipe.
Further technical scheme, described three-way pipe are Y-shaped three-way pipe, and described three-way pipe is noted with sampling needle, machinery
The pipeline of the injection needle connection of injection device is provided with pinch valve;Pinch valve has two, respectively normally opened pinch valve and often pass clamp
Valve.When syringe is pulled up, normally opened pinch valve is opened, and normally closed pinch valve is closed, and completes the extraction of sample liquid;When syringe is pushed,
Normally opened pinch valve is closed, and normally closed pinch valve is opened, completes sample liquid and be injected into sample storage device.
Further technical scheme, described three-way pipe are fixed by pinch valve, and pinch valve is fixed on pipe clamp.
Further technical scheme, the sample storage device include reducing motor, bearing block, rotation dish, sample box, every
Bottle stopper plate;Described sample box is in rotation dish;Described reducing motor is connected with sample box support;Described bearing block is solid
It is scheduled on sample box support, described rotation dish is connected with the screw mandrel of reducing motor, described is fixed on sample box every bottle stopper plate
On support, positioned at the surface at sample box center, described reducing motor is connected with control device.When injection needle is from every bottle stopper
When the downward fluid injection of plate is to sample cell, sample cell pipe cap can be avoided from coming off with the rising of injection needle.
Further technical scheme, described sample box is provided with sample pore, for placing sample cell.Sample cell is placed on
On sample pore, the injection of injection needle is waited.
Further technical scheme, also includes Hall element, described hall sensing in described sample storage device
Device is fixed on sample box mid-stent position on the lower side, and the bottom of sample box is fixed with fritter located at the bottom of sample box
Magnet, when sample collecting apparatus electrification reset, control device judges the position of magnet by Hall element, completes sample box
The reset of original position.
Further technical scheme, the cleaning device include Washing cup, membrane pump, level sensor, connect liquid bottle, waste liquid
Bottle;Described membrane pump includes that the first membrane pump and the second membrane pump, the first described membrane pump extract the cleaning connect in liquid bottle
Liquid, the waste liquid in described the second membrane pump extraction Washing cup is into waste liquid bottle.Described level sensor is located at and connects liquid bottle one
Side, connects the cleaning fluid surplus in liquid bottle for monitoring, and the first described membrane pump and the second membrane pump are connected with control device.
While injection needle moves into Washing cup, the first barrier film pump work so that cleaning fluid rinses injection needle.Meanwhile, the second membrane pump
Work, the waste liquid extracted in Washing cup are entered in waste liquid bottle.When connecing the cleaning fluid in liquid bottle and being not enough, level sensor can
Monitor the remaining cleaning fluid volume of cleaning fluid and be uploaded to control device and send warning operation.
Further technical scheme, described Washing cup electroplate sheath.
Further technical scheme, described on-line sample harvester also include ultraviolet sterilization device, and described is ultraviolet
Sterilizing unit is located at the side of injection needle.
Further technical scheme, described sample cell are vacuum test tube.
Further technical scheme, a kind of on-line sample acquisition and storage device, the user of described sample collecting apparatus
Method is:
Step one:Start syringe by the sample liquid in sampling needle collect specimen into syringe, then by syringe
Sample liquid be expelled in sample, repeat the above steps;
Step 2:Pushing syringe, the waste liquid in the pipeline that syringe is connected with injection needle are discharged;Described waste liquid row
Go out and can be expelled in Washing cup, while being cleaned to injection needle syringe needle using cleaning fluid;
Step 3:After waste liquid is completely exhausted out, sample liquid is injected to injection needle, collects sample liquid.
Further technical scheme, the number of repetition of described step one is at least one times.
Further technical scheme, further technical scheme, the amount of the sample liquid in described syringe is more than wanted
Collect the amount of sample liquid.
When the circulation fluid path of microorganism culture works, control device sends sample collection and instructs to sample collecting apparatus,
Wherein syringe is made pull-up by oneself and pushes twice i.e. pump drainage and twice, carries out the mixed of microbial culture medium in the culture pipeline of incubator
Even, then carry out sample collection.During per injection device pump drainage, sample is entered in syringe, during pressure in syringe by gathering pin
Sample liquid is pushed in sample by gathering pin, is confused with sample, is then proceeded to take out sample with syringe, then press, at least twice
After taking out pressure, in syringe, there is no the sample liquid residual of last collection, the sample liquid for being collected is the sample of this collection
Liquid.
General once sampling needs two sample cells to collect sample, in order to realize the pre- discharge opeing operation of syringe, sample liquid
Extraction amount need the quantitative values more than two sample cells.After sample liquid enters injection needle, injection needle elder generation removal waste fluid, main mesh
Be will last sampling when the waste liquid that remain in injection needle discharge.Injection needle is discharged to waste liquid in Washing cup, makes
Injection needle is cleaned with cleaning fluid.After the cleaning of cleaning fluid, injection needle is moved by mechanical arm, then by the electricity that slows down
Machine rotates sample disc, realizes injection needle and new sample cell, being aligned every bottle plate washer aperture, and syringe is pressed still further below, completes to note
Injection and storage operation that sample liquid in emitter is entered in sample cell.
Sample liquids after collection are stored in 4 degrees Celsius of sample storage environment.Can be used for the counting inspection of cell, bacterium liquid
Survey or the detection for liquor strength.
Beneficial effect:
1st, a kind of on-line sample acquisition and storage device of the present utility model can realize automatic sampling, storage and cleaning behaviour
Make, it is to avoid the artificial authenticity for operating caused efficiency, security, monitoring is low, and the device is simple to operate, and efficiency is higher, peace
Full property is also higher.
2nd, the utility model Washing cup surface is provided with silver-plated sheath, can reduce germ contamination, keeps Washing cup itself
Gnotobasis, and silver-plated with low cost, effective time is long, lifts the cleaning performance of injection needle.
3rd, mechanical arm of the present utility model can realize injection needle, every bottle stopper plate, the alignment of sample pore;Injection needle is downward
During motion, alignment insertion sample cell aperture;After injection needle injection sample cell, injection needle is moved in Washing cup and is cleaned,
Automatic sample can be realized, and is directed at automatically sample cell.
4th, reset of the control device of the present utility model by optocoupler signal control motor, it is to avoid the piston of syringe is pressed onto
Bottom.
5th, automation sample collecting apparatus of the present utility model reduce production cost, and eliminate labyrinth and bring
High fault rate problem.
6th, of the present utility model can separate vacuum specimen tube and injection needle every bottle stopper plate, prevent injection needle under subnormal ambient
After piercing vacuum specimen tube, frictional force is larger and rubber surface plug between, pull-up vacuum specimen tube.
7th, Hall element of the present utility model has the feature of stable performance, accurate positioning, carries little magnetic for bottom
The initial reset of the sample box of block.Especially in the case of 4 degrees Celsius of preservations of sample, condensed water, hall sensing can be produced
Device compares the original papers such as infrared sensor with the characteristic not affected by condensed water, stably can play a role.
8th, the membrane pump in the utility model can be in severe working environment, during such as discharge of wastewater, due to sewage
In impurity is more and complicated, pipeline is easy to block, and so just produces the too high situation of load to electric pump, and the motor feels hot and easily
Loss, and pneumatic diaphragm pump can pass through particle and flow is adjustable, is automatically stopped during line clogging to unobstructed.Level sensor can
Implementing monitoring, preventing cleaning fluid to lack causes the situation of cleaning failure, sample contamination to occur.And can monitor automatically, reduce
Cost and region in possibility of pollution is manually checked.
9th, ultraviolet-sterilization of the present utility model is different from other sterilization modes, and there is good sterilizing function to air, and
Safety non-pollution, can be controlled at any time, be arranged on beside injection needle and can avoid causing damage the sample in glass tube with vacuum.
10th, the utility model sample cell adopts vacuum specimen tube, can avoid polluting, and is enclosed environment, more conventional adopts
Sample pipe, vacuum specimen tube manually need not close the lid, and be prevented effectively from pollution, keep sample data authenticity.
11st, during collection sample liquid, the sample liquid that last time can be remained in syringe is carried out the utility model
Pump drainage, can be discharged into the waste liquid remained in pipeline in culture vessel in advance at least twice, through noting after pump drainage twice
The sample liquid that emitter is finally extracted into is consistent with sample.One section liquid is first discharged discharging when sample liquid enters sample cell
Body, then cleans injection needle, can remove the possibility of last time sample carryover.Through the step, detection can be significantly improved accurate
True rate, and syringe need not be changed, reduce equipment cost.Advantageously ensure that in the sample liquid of collection without last time sample
Residual, and then improve experiment Detection accuracy.
Description of the drawings
Fig. 1 is structural representation of the present utility model
Fig. 2 is the front view of sample collecting apparatus in the utility model;
Fig. 3 is the rearview of sample collecting apparatus in the utility model;
Fig. 4 is sample storage device in the utility model, the structural representation of cleaning device;
Fig. 5 is medicine calibration curve in the utility model embodiment;
Mark in figure:1st, sample collecting apparatus, 2, sample storage device, 3, cleaning device, 4, mechanical injection device, 5, in
Control device, 6, ultraviolet sterilization device;
1-1, syringe, 1-2, sampling needle, 1-3, fixed plate, 1-4, push-and-pull slide block, 1-5, V-block, 1-6, depression bar combination
Part, 1-7, line slideway, 1-8, lead screw motor, 1-9, feed screw nut's connecting plate, 1-10, optocoupler catch, 1-11, optocoupler, 1-12,
Three-way pipe, 1-13, pinch valve, 1-14, pipe clamp;1-15, injection pipeline;1-16 sample lines;1-17 cultivates liquid pipeline;
2-1, reducing motor, 2-2, bearing block, 2-3, rotation dish, 2-4, sample box, 2-5, sample box support, 2-6, every bottle
Baffle plate, 2-7, Hall element, 2-8, sample cell;
3-1, Washing cup, 3-2, the first membrane pump, 3-3, the second membrane pump, 3-4, level sensor, connect liquid bottle at 3-5,
3-6, waste liquid bottle;
4-1, mechanical arm, 4-2, pin folder, 4-3, injection needle.
Specific embodiment
With reference to the accompanying drawings and examples the utility model is described in further detail.
Embodiment
As shown in Figures 1 to 4, a kind of on-line sample acquisition and storage device, including sample collecting apparatus 1, sample storage device
2, cleaning device 3, mechanical injection device 4 and control device 5;Sample collecting apparatus 1 are connected with mechanical injection device 4, and will pipe
The sample liquid extracted in road is injected in mechanical injection device 4;Cleaning device 3 enters to the injection needle 4-3 in mechanical injection device 4
Row sterilization cleaning;Mechanical injection device 4 is by sample liquid injection sample storage device 2;Described control device 5 and harvester
1, sample storage device 2, cleaning device 3, mechanical injection device 4 are connected.
Described mechanical injection device 4 includes mechanical arm 4-1, pin folder 4-2 and injection needle 4-3;Described mechanical arm 4-1 leads to
Cross pin folder 4-2 to be connected with injection needle 4-3, described mechanical arm 4-1 controls motions of the injection needle 4-3 in space, described machine
Tool arm 4-1 is connected with control device 5.Mechanical arm 4-1 can realize injection needle 4-1, every bottle stopper plate 2-6, the alignment of sample pore;
When injection needle 4-3 is moved downward, alignment insertion sample cell aperture;After injection needle 4-3 injection sample cell 2-8, by injection needle
4-3 is moved in Washing cup 3-1 and is cleaned.
The sample collecting apparatus 1 include syringe 1-1, sampling needle 1-2, fixed plate 1-3, push-and-pull slide block 1-4, V-block
1-5, depression bar sub-assembly 1-6, line slideway 1-7, lead screw motor 1-8, feed screw nut connecting plate 1-9, optocoupler catch 1-10, optocoupler
1-11, three-way pipe 1-12;The piston handle of described syringe 1-1 is fixed on line slideway 1-7 by push-and-pull slide block 1-4,
Line slideway 1-7 is fixed on the top of fixed plate 1-3;Described optocoupler catch 1-10 is arranged on feed screw nut connecting plate 1-9,
Optocoupler 1-11 is installed in the middle part of fixed plate 1-3;Control device 5 passes through the reset for judging optocoupler signal control motor, it is to avoid note
The piston of emitter 1-1 is pressed onto bottom.
The staving of described syringe 1-1 is fixed on the bottom of fixed plate 1-3 by depression bar sub-assembly 1-6, V-block 1-5;
Described lead screw motor 1-8 is connected with push-and-pull slide block 1-4 by feed screw nut's connecting plate 1-9;Push-and-pull slide block 1-4 can be in screw mandrel
Move up and down in slide rail under the drive of motor 1-8, and then drive pull-up and the depression of syringe 1-1.Described screw mandrel electricity
Machine 1-8 is connected with control device 5.Described syringe 1-1 is by three-way pipe 1-12 and sampling needle 1-2, mechanical injection device 4
Injection needle 4-3 connects.
Described three-way pipe 1-12 is Y-shaped three-way pipe, described three-way pipe and sampling needle 1-2, the note of mechanical injection device 4
The pipeline for penetrating pin 4-3 connections is provided with pinch valve 1-13;Pinch valve has two, respectively normally opened pinch valve and often pass pinch valve.
When syringe is pulled up, normally opened pinch valve is opened, and normally closed pinch valve is closed, and completes the extraction of sample liquid;When syringe is pushed, normally opened
Pinch valve is closed, and normally closed pinch valve is opened, completes sample liquid and be injected into sample storage device.Described three-way pipe 1-12 passes through pinch valve
1-13 is fixed, and pinch valve 1-13 is fixed on pipe clamp.
The sample storage device 2 includes reducing motor 2-1, bearing block 2-2, rotation dish 2-3, sample box 2-4, sample box
Support 2-5, every bottle stopper plate 2-6;Described sample box 2-4 is in rotation dish 2-3;Described reducing motor 2-1 and sample box
Support 2-5 connects;Described bearing block 2-2 is fixed on sample box support 2-5, described rotation dish 2-3 and reducing motor 2-1
Screw mandrel connection, described is fixed on sample box support 2-5, positioned at the surface at sample box 2-4 centers every bottle stopper plate 2-6,
Described reducing motor 2-1 is connected with control device 5.When injection needle 4-3 is from every the downward fluid injections of bottle stopper plate 2-6 to sample cell 2-
When 8, sample cell pipe cap can be avoided from coming off with the rising of injection needle.Described sample box 2-4 is provided with sample pore, for putting
Put sample cell 2-8.Sample cell 2-8 is placed on sample pore, waits the injection of injection needle 4-3.
Also include in described sample storage device 2 that Hall element 2-7, described Hall element 2-7 are located at sample
The bottom of box 2-4, is fixed on position on the lower side in the middle part of sample box support 2-5, and the bottom of sample box 2-4 is fixed with fritter magnetic
Iron, when 1 electrification reset of sample collecting apparatus, control device 5 judges the position of magnet by Hall element 2-7, completes sample
The reset of product box 2-4 original positions.
The cleaning device 3 includes Washing cup 3-1, membrane pump, level sensor 3-4, meets liquid bottle 3-5, waste liquid bottle 3-6;
Described membrane pump includes that the first membrane pump 3-2 and the second membrane pump 3-3, the first described membrane pump 3-2 are extracted and met liquid bottle 3-5
In cleaning fluid, described the second membrane pump 3-3 extracts waste liquid in Washing cup 3-1 into waste liquid bottle 3-6.Described liquid level sense
Device 3-4 is answered located at connecing liquid bottle 3-5 sides, the cleaning fluid surplus in liquid bottle is connect for monitoring, the first described membrane pump 3-2 and the
Two membrane pump 3-3 are connected with control device 5.While injection needle 4-3 moves into Washing cup 3-1, the first membrane pump 3-2 works
Make so that cleaning fluid rinses injection needle 4-3.Meanwhile, the second membrane pump 3-3 works, and the waste liquid entrance that extracts in Washing cup 3-1 is useless
In liquid bottle 3-6.When connecing the cleaning fluid in liquid bottle 3-5 and being not enough, level sensor 3-4 can monitor the remaining cleaning fluid of cleaning fluid
Measure and be uploaded to control device 5 and send warning operation.Described Washing cup 3-1 electroplate sheaths.
Described on-line sample harvester 1 also includes ultraviolet sterilization device 6, and described ultraviolet sterilization device is located at injection
The side of pin 4-3.
Described sample cell is vacuum test tube.
A kind of on-line sample acquisition and storage device, the using method of described sample collecting apparatus is:
Step one:In by the sample liquid in sampling needle collect specimen to syringe, then the sample liquid in syringe is arranged
In going out to sample, above-mentioned steps are repeated once;
Step 2:Pushing syringe, the waste liquid in the pipeline that syringe is connected with injection needle are discharged;Described waste liquid row
Go out and can be expelled in Washing cup, while being cleaned to injection needle syringe needle using cleaning fluid;
Step 3:After waste liquid is completely exhausted out, sample liquid is injected to injection needle, collects sample liquid.
A kind of verification experimental verification of the pull effect of on-line sample harvester.
Test prepares:
1st, the Ciloxan of high concentration is configured(Mother liquor) 1000.5 mg/L.
2nd, prepare the pure water of 4L or so, with the dilution of the hydrochloric environment sand star solution in pipeline to be recycled.
Process of the test:
1)The fluid path of circulation is built, by the use of culture medium bag as container, pump is formed by pipeline and looped back as power
Road.Culture medium bag is located on wobbler, accelerates to mix.The pure water of 1L is injected in culture medium bag, 10min is waited so that circulation
Loop stability.Sampling needle is located at pipeline.
Being mixed in 1L samples completely needs the time of 10 minutes, is sampled detection within 20 minutes after the completion of sample-adding.
Start the clock after fluid path stable circulation, initial time is 0, sampling detection.
2)In order to reach drug concentration 8.06mg/L, after operation, 10 minutes injection mother liquor amounts are 8.06 ml;Sample within 30 minutes
Detection
3)In order to reach drug concentration 11.49mg/L, it is 3.43ml that 40 minutes after operation continue injection mother liquor amount.60 minutes
When sampling detection;
4、)In order to reach drug concentration 15.96mg/L, it is 4.47ml that 70 minutes after operation continue injection mother liquor amount.90 points
Clock samples detection during minute;
5)In order to reach drug concentration 11.29mg/L, first discharge mixed liquor 293ml within 100 minutes after operation, continue injection pure
Water 293ml.Sampling detection during 120 minute minute;
6)In order to reach drug concentration 7.2mg/L, first discharge mixed liquor 362ml within 130 minutes after operation, continue injection pure water
362ml.To be diluted.Sampling detection during 150 minute minute;
7)In order to reach drug concentration 3.95mg/L, first discharge mixed liquor 452ml within 160 minutes after operation, continue injection pure
Water 452ml, to be diluted.Sampling detection during 180 minute minute;
8)In order to reach drug concentration 2.51mg/L, first discharge mixed liquor 364ml within 190 minutes after operation, continue injection pure
Water 364ml, to be diluted sampling detection during 210 minute minute;
9)In order to reach drug concentration 1.65mg/L, first discharge mixed liquor 342ml within 220 minutes after operation, continue injection pure
Water 342ml.To be diluted sampling detection during 240 minute minute;
Sample collection:
In each moment point, direct drawing liquid sampling is carried out using our on-line sample harvester(Do not mix, not walkthrough
Waste liquid)Sample with drawing liquid is mixed(2 mixings of pull, pre- removal waste fluid).Then sampled using 723N visible spectrophotometers
The measure of liquor strength afterwards, the absorbance of test sample sample.By calculating comparative drug(Ciprofloxacin Hydrochloride)Suction
Shading value, detects the concentration of comparative drug.
During experiment, detection uses spectrophotometer to start shooting before being detected and preheats 20-30 min, then by wavelength
It is transferred to 320 nm.Returned to zero with deionized water as blank.The variable concentrations of the mother liquor of detection Ciloxan
The absorbance of dilution, duplicate detection is twice.
Obtain calibration curve Fig. 5 of absorbance and the corresponding drug concentration of Ciprofloxacin Hydrochloride.
After direct sampling is sampled and mixes drawing liquid sampling, detect by the number order of vacuum test tube molten in each sampling pipe
The absorbance of liquid, then according to " pharmaceutical standards curve ", removes the drug concentration of the anti-sample solution for releasing Ciprofloxacin Hydrochloride.
Experimental result:
Through actual testing inspection, two kinds of microbiological specimens systems that pull is mixed and application pull is mixed are not applied
The comparing result of the drug concentration of the Ciloxan of system, as described in following table one.
The two kinds of drug concentration contrasts that pull is not mixed and pull is mixed of table 1
The Blending Efficiency of Blending that can significantly improve medicine using mixing pull for 2 times is can be seen that from upper table and Fig. 5, is detected
Drug concentration closer to actual liquor strength Configuration Values.