CN211263338U - Two-dimensional liquid chromatograph capable of reducing matrix effect of LC-MS - Google Patents
Two-dimensional liquid chromatograph capable of reducing matrix effect of LC-MS Download PDFInfo
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- CN211263338U CN211263338U CN201922080445.6U CN201922080445U CN211263338U CN 211263338 U CN211263338 U CN 211263338U CN 201922080445 U CN201922080445 U CN 201922080445U CN 211263338 U CN211263338 U CN 211263338U
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Abstract
The utility model provides a can reduce two-dimentional liquid chromatograph of LC-MS instrument matrix effect, include: a first flow channel, a second flow channel, a third flow channel, an analysis flow channel connected with a second chromatographic column (C2), a waste liquid flow channel (L7), a first multi-flow channel switching valve (V1) and a second multi-flow channel switching valve (V2) which are connected, an AIR flow channel connected with an AIR filter (AIR) and an AIR compression pump (P3), and the like. The utility model discloses an introduce compressed air's mode, with first chromatographic column and in its continuous pipeline in first mobile phase discharge apparatus to reduce or all get rid of first mobile phase and get into the production of mass spectrum part and lead to the matrix effect, avoid the harmful effects to response, stability and the reproducibility of mass spectrum.
Description
Technical Field
The utility model belongs to liquid chromatography-mass spectrometer instrument equipment field, concretely relates to can reduce two-dimensional liquid chromatograph of liquid chromatography-mass spectrometer matrix effect.
Background
Two-dimensional liquid chromatography is a chromatographic instrument developed on the basis of ordinary liquid chromatography, and improves the separation capacity by increasing the number of chromatographic separation stages and the number of chromatographic columns. Since the liquid chromatography cannot directly obtain the structural information of the target object, the unknown object must be judged by comparing with the standard object, and different detectors are usually required to be replaced for special substances, such as: for compounds without ultraviolet absorption, common ultraviolet absorption detectors cannot be used for detection, and a fluorescence detector, a differential detector or an electrochemical detector and the like are required to be replaced; on the other hand, the method is limited by detection limits of various detectors, the two-dimensional liquid phase has obvious limitations in trace analysis, the mass spectrum detector has the advantages of wide analysis range (almost all compounds can be detected), strong separation capability, reliable qualitative analysis result, low detection limit, short analysis time and the like, and the liquid chromatography-mass spectrum combination is widely applied to a plurality of fields such as drug analysis, food analysis, environmental analysis and the like.
The mass spectrometer is limited by the technical defects of the mass spectrometer, and the mass spectrometer is required to purify a sample to be analyzed and can analyze the sample after having certain purity. Separating the sample in the mass spectrum part and the flowing phase, ionizing, and separating ion fragments by mass through a mass analyzer of the mass spectrum to obtain a mass spectrum. After chromatographic separation, the co-elution substances enter the mass spectrum part together, so that the ionization efficiency of the component to be detected is changed, and the signal inhibition or improvement caused by the co-elution substances is called matrix effect. The occurrence of matrix effects is considered to be a significant obstacle to the liquid chromatography-mass spectrometry technique, since it largely impairs the stability of the response and reproducibility of the results.
In the existing two-dimensional liquid chromatography-mass spectrometer, in order to remove endogenous impurities of a biological sample in a first dimension as much as possible, ion pair reagents, buffer salts and the like are inevitably needed, and the substances are often transferred to the two-dimensional chromatogram along with a target object to different degrees and then enter a mass spectrum part to cause the generation of matrix effect, so that the response, stability and reproducibility of the mass spectrum are influenced, and the application of the two-dimensional liquid chromatography-mass spectrometer in conventional detection is limited.
SUMMERY OF THE UTILITY MODEL
For solving the technical problem in the background, the utility model aims at improving a two-dimensional liquid chromatograph, reducing or eliminating the matrix effect that two-dimensional liquid chromatograph brought for the mass spectrometer.
The utility model discloses a to introduce compressed air's mode, with first chromatographic column and in its first mobile phase discharge apparatus who links to each other in the pipeline to reduce or all get rid of first mobile phase and get into the mass spectrum part and lead to the production of matrix effect.
The purpose of the utility model is realized through the following technical scheme: a two-dimensional liquid chromatograph capable of reducing effects of a hplc matrix, comprising: a first flow channel for conveying a first mobile phase; a second flow channel for conveying a second mobile phase;
an analysis flow channel connected with a second chromatographic column and used for separating and detecting the captured substances; a waste liquid flow passage for discharging waste liquid; the first multi-channel switching valve and the second multi-channel switching valve are connected and used for connecting and switching each channel; the first multi-channel switching valve and the second multi-channel switching valve are provided with a plurality of ports; the air flow channel is connected with an air filter and an air compression pump; one port of the first multi-channel switching valve is provided with a plug, and any two other ports are respectively connected with the first channel and the air channel; a first chromatographic column is connected between any two ports of the second multi-channel switching valve; any three ports of the second multi-channel switching valve are also respectively connected with a second channel, a waste liquid channel and an analysis channel.
The first multi-channel switching valve is provided with 4 ports which are respectively a port 1, a port 2, a port 3 and a port 4; the second multi-channel switching valve is provided with 6 ports, namely a port 5, a port 6, a port 7, a port 8, a port 9 and a port 10; the first flow channel is connected with the port 1, the air flow channel is connected with the port 3, the port 2 is provided with a plug, the port 4 is connected with the port 6, the flow channels of the port 7 and the port 10 are connected with the first chromatographic column, the port 8 is connected with the analysis flow channel, and the port 9 is connected with the second flow channel.
The preferred scheme is as follows: the device also comprises a third flow channel for conveying a third mobile phase, and the third flow channel is connected to the first flow channel. As only the first mobile phase in the first chromatographic column and the connected pipeline is discharged out of the instrument, part of inorganic salt or substances with weak ionic strength still remain in the chromatographic column, and the third mobile phase is introduced to wash the first chromatographic column and the connected pipeline, the related substances can be further reduced from entering a mass spectrum part, so that the matrix effect of the two-dimensional liquid chromatography on a mass spectrum detector is reduced or eliminated.
And the first flow channel is provided with a first chromatographic pump and a sample injector.
Further preferably, the third flow channel is connected to the first flow channel located at the rear end of the sample injector.
And a second chromatographic pump is arranged on the second flow channel.
The analysis flow channel is connected to a detector.
The air compression pump enables the pressure in the pipeline to reach a set value of 1MPa-1.2 MPa.
The air filter can filter out particulate matters with the diameter larger than 0.2 micron in the compressed air.
The third flow may be any solvent that can dissolve salts and is difficult to ionize, such as water, acid, and the like.
The utility model discloses for prior art have following advantage and effect:
1. the utility model discloses a can reduce two-dimensional liquid chromatograph of LC-MS matrix effect through introducing compressed air with first chromatographic column and the first mobile phase discharge instrument in its continuous pipeline to reduce or all get rid of first mobile phase and get into the mass spectrum part and lead to the production of matrix effect.
2. The utility model discloses a can reduce two-dimensional liquid chromatograph that LC-MS matrix effect filters the diameter that filters in the compressed AIR through AIR AIR cleaner and is greater than 0.2 micron's particulate matter, improves compressed AIR's purity, reduces the influence of impurity to equipment, lifting means life.
3. The utility model discloses a can reduce two-dimensional liquid chromatograph of LC-MS matrix effect washes first chromatographic column and continuous pipeline through introducing the third mobile phase, can further reduce the material that partial inorganic salt or ionic strength are weak and get into the mass spectrum part to reduce or eliminate the production of two matrix effects, avoid the harmful effects to response, stability and the reproducibility of mass spectrum.
4. The utility model discloses a can reduce two-dimensional liquid chromatograph of LC-MS matrix effect adds the third mobile phase increase volume when advancing the appearance, and the volume 50 microliters are advanced to the biggest appearance usually when not adding, reaches 1 milliliter at advancing the appearance volume after increasing the third mobile phase, obtains the punctiform effect of advancing of ideal, reduces and advances a kind error, improves and advances kind accuracy and repeatability.
Drawings
FIG. 1 is a schematic view of the structure of a liquid chromatograph in example 1;
fig. 2 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 1;
fig. 3 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 1;
fig. 4 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 1;
FIG. 5 is a schematic view of the structure of a liquid chromatograph in example 2;
fig. 6 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 2;
fig. 7 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 2;
fig. 8 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 2;
fig. 9 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 2;
fig. 10 is an operation state diagram of the two-dimensional liquid chromatograph of embodiment 2;
wherein, S1 first mobile phase, S2 second mobile phase, S3 third mobile phase; p1 first chromatography pump, P2 second chromatography pump, P3 air compressor pump, P4 third chromatography pump; a first section of an L1 first flow channel, a second section of an L2 first flow channel, a third section of an L3 first flow channel, a first connecting pipeline of an L4, a second connecting pipeline of an L5, a third connecting pipeline of an L6, an L7 waste liquid flow channel, a first section of an L8 second flow channel, a second section of an L9 second flow channel, a first section of an L10 analyzing flow channel, a second section of an L11 analyzing flow channel, a first section of an L12 air line, a second section of an L13 air line, a first section of an L14 third flow channel, and a second section of an L15 third flow channel; v1 first multi-channel switching valve; v2 second multi-channel switching valve; a T1 plug; c1 first chromatography column; a C2 second chromatography column; a W1 waste port; a D1 detector; SIL autosampler; AIR filters; J1T-junction; the thick solid lines in the figure represent flowing phase flow paths and the dashed lines represent non-flowing lines.
Detailed Description
The invention will be further explained and explained with reference to the drawings and examples.
Example 1
A two-dimensional liquid chromatograph capable of reducing effects of a hplc matrix, comprising: a first flow path for conveying a first mobile phase S1; a second flow path for conveying a second mobile phase S2; an analysis flow channel connected with a second chromatographic column C2 for separating and detecting the captured substance; a waste liquid flow path L7 for discharging waste liquid; a first multi-channel switching valve V1 and a second multi-channel switching valve V2 connected to each other for connecting and switching the channels; a plurality of ports are arranged on the first multi-channel switching valve V1 and the second multi-channel switching valve V2; the AIR purifier also comprises an AIR flow channel connected with an AIR filter AIR and an AIR compression pump P3; one port of the first multi-channel switching valve V1 is provided with a plug T1, and any two other ports are respectively connected with the first channel and the air channel; a first chromatographic column C1 is connected between any two ports of the second multi-channel switching valve V2; any three ports of the second multi-channel switching V2 valve are respectively connected with a second channel, a waste liquid channel L7 and an analysis channel.
As shown in fig. 1, the first multi-channel switching valve has 4 ports, which are port 1, port 2, port 3 and port 4; the second multi-channel switching valve is provided with 6 ports, namely a port 5, a port 6, a port 7, a port 8, a port 9 and a port 10; the first flow channel is connected with a port 1, the air flow channel is connected with a port 3, a plug T1 is arranged on the port 2, the port 4 is connected with a port 6, a first chromatographic column C1 is connected on the flow channels of the port 7 and the port 10, the port 8 is connected with an analysis flow channel, and the port 9 is connected with a second flow channel.
As shown in fig. 2, the first multi-way switching valve V1 is in the position state of 1-way 4, 2-way 3, and the second multi-way switching valve V2 is in the position state of 5-way 10, 6-way 7, 8-way 9.
The AIR compression pump P3 is started and delivers the AIR filtered by the AIR filter AIR to the second L13 pipeline, and the AIR compression pump P3 stops working after the pressure in the pipeline reaches the set value (1Mpa) because the port 2 of the first multi-channel switching valve V1 uses the plug T1 to plug the channel. Compressed AIR input into the equipment through the P3 AIR pump needs to pass through an AIR AIR filter to filter out particulate matters with the diameter larger than 0.2 micron in the AIR, so that the service life of the equipment is prolonged.
A first mobile phase S1 is conveyed by a first chromatographic pump P1 to pass through a first section L1 of a first flow channel and a second section L2 of the first flow channel, carries a sample by an autosampler SIL, passes through a third section L3 of the first flow channel, a first connecting pipeline L4 and a second connecting pipeline L5, and leads to a first chromatographic column C1 to carry out primary separation on the sample; impurities remaining on the first chromatographic column C1 and having a strength less than that of the target first pass through the third connecting line L6, the waste liquid flow channel L7, and then exit the apparatus through the waste liquid port W1.
As shown in fig. 3, the first multi-channel switching valve V1 was switched to 1-pass 2 and 3-pass 4 before the target and impurities having similar retention strength to the target on the first column C1 were eluted from the first column C1. The position of the second multi-flow passage switching valve V2 is not changed.
Simultaneously, the first chromatographic pump P1 stops working, the conveying of the first mobile phase S1 is stopped, and compressed air passes through the second section L13 of the air pipeline, the first connecting pipeline L4, the second connecting pipeline L5, the third connecting pipeline L6 and the waste liquid channel pipeline L7, and the pipeline and the first mobile phase S1 in the first chromatographic column C1 are discharged out of the instrument through a waste liquid port W1; when the pressure of the compressed air in the pipeline is lower than the set value of 1Mpa, the compressed air pump P3 is automatically started to maintain the pressure in the pipeline.
As shown in fig. 4, when all of the second connecting line L5, the third connecting line L6, and the first mobile phase S1 in the first chromatographic column C1 were discharged from the apparatus by compressed air, the first multi-channel switching valve V1 was set to 1-pass 4, 2-pass 3, and the second multi-channel switching valve V2 was set to 5-pass 6, 7-pass 8, 9-pass 10.
At this time, the first chromatographic pump P1 delivers the first mobile phase S1 through the first flow channel first section L1, the first flow channel second section L2, the first flow channel third section L3, the first connecting line L4 and the waste flow channel L7, and the first mobile phase S1 is stopped to be delivered after the discharge device or the first chromatographic pump P1 is suspended through the waste flow port W1.
Meanwhile, the second chromatographic pump P2 delivers the second mobile phase S2 to pass through the second flow channel first section L8, the second flow channel second section L9, and the second connecting line L5, to the first chromatographic column C1, and elutes the target from the first chromatographic column C1, passes through the third connecting line L6, the analysis flow channel first section L10, to the second chromatographic column C2 to complete separation, and passes through the analysis flow channel second section L11 to enter the detector D to complete detection.
Example 2
As shown in fig. 5, a two-dimensional liquid chromatograph capable of reducing the effect of the hplc matrix further includes a third flow channel for delivering a third mobile phase S3 on the basis of embodiment 1; and the third flow channel is connected to the first flow channel and is positioned on the flow channel at the rear end of the sample injector. Since only the first chromatographic column C1 and the first mobile phase S1 in the connected pipeline are discharged out of the instrument, part of inorganic salts or substances with weak ionic strength still remain in the chromatographic column, and the third mobile phase S3 is introduced to flush the first chromatographic column C1 and the connected pipeline, so that the related substances can be further reduced to enter a mass spectrum part, and the matrix effect of two-dimensional liquid chromatography on a mass spectrum detector is reduced or eliminated.
As shown in FIG. 6, the first multi-channel switching valve V1 is in the position of 1-through 4, 2-through 3, and the second multi-channel switching valve V2 is in the position of 5-through 10, 6-through 7, 8-through 9.
The AIR compressor pump P3 is started and delivers the AIR filtered by the AIR filter AIR to the second segment L13 of the AIR line, and since the port 2 of the first multi-channel switching valve V1 uses the plug T1 to block the passage, the AIR compressor pump P3 stops working after the pressure in the line reaches the set value (1 Mpa).
The first mobile phase S1 is delivered by the first chromatographic pump P1 to pass through the first section L1 of the first flow channel and the second section L2 of the first flow channel, and the sample is carried by the autosampler SIL, and the third mobile phase S3 is delivered by the third chromatographic pump P4 to pass through the third section L3 of the first flow channel, the first connecting pipeline L4 and the second connecting pipeline L5 after being mixed with the first mobile phase S1 by the tee joint J1, and then the mixture is led to the first chromatographic column C1. The third mobile phase S3 is added during sample injection to increase the volume of sample injection, the maximum sample injection volume is usually 50 microliter when not added, and the sample injection volume reaches 1 milliliter after the third mobile phase S3 is added, so that an ideal point sample injection effect can be still obtained, and the sample injection error can be reduced.
As shown in fig. 7, after the sample injection is completed, the third chromatographic pump P4 is suspended and the third mobile phase S3 is stopped. The first mobile phase S1 delivered by the first chromatographic pump P1 performs a primary separation of the sample through the first chromatographic column C1; impurities with the strength smaller than that of the target substance remaining on the first chromatographic column C1 are discharged from the apparatus through the waste liquid port W1, first through the third connecting line L6, the waste liquid flow channel L7.
As shown in fig. 8, the first multi-channel switching valve V1 is switched to 1-pass 2, 3-pass 4 before the target and impurities having similar retention strength to the target on the first chromatographic column C1 are eluted from the first chromatographic column C1. The position of the second multi-flow passage switching valve V2 is not changed.
Simultaneously, the first chromatographic pump P1 and the third chromatographic pump P4 stop working, the delivery of the first mobile phase S1 and the third mobile phase S3 is stopped, compressed air passes through the second section L13 of the air pipeline, the first connecting pipeline L4, the second connecting pipeline L5, the third connecting pipeline L6 and the waste liquid channel L7, and the pipeline and the first mobile phase S1 in the first chromatographic column C1 are discharged out of the instrument through the waste liquid port W1; when the pressure of the compressed air in the pipeline is lower than the set value of 1Mpa, the compressed air pump P3 is automatically started to maintain the pressure in the pipeline.
As shown in fig. 9, when all of the second connecting line L5, the third connecting line L6, and the first mobile phase S1 in the first chromatographic column C1 were discharged from the apparatus by compressed air, the position state of the first multi-channel switching valve V1 was 1-pass 4, 2-pass 3, and the position state of the second multi-channel switching valve V2 was maintained.
At this time, the first chromatography pump P1 is still suspended and does not deliver the first mobile phase S1. And meanwhile, the third chromatographic pump P4 is started, the third mobile phase S3 is conveyed to pass through the third flow channel first section L14, the third flow channel second section L15, the first flow channel third section L3 and the first connecting pipeline L4, inorganic salt and other substances which are difficult to ionize and the like in the first mobile phase S1 remained in the second connecting pipeline L5, the third connecting pipeline L6 and the first chromatographic column C1 are eluted, and the substances pass through the third connecting pipeline L6 and the waste liquid flow channel L7 and are discharged out of the instrument through the waste liquid port W1.
After the substances, such as inorganic salts, which are difficult to ionize in the first mobile phase S1 remaining in the second connecting line L5, the third connecting line L6, and the first chromatographic column C1 are eluted, the state in which the compressed air is discharged out of the first mobile phase S1 is repeated, and the third mobile phase S3 in the second connecting line L5, the third connecting line L6, and the first chromatographic column C1 is discharged out of the apparatus.
As shown in fig. 10, when all of the second connecting line L5, the third connecting line L6, and the third mobile phase S3 in the first chromatographic column C1 were discharged from the apparatus by compressed air, the first multi-channel switching valve V1 was set to 1-pass 4, 2-pass 3, and the second multi-channel switching valve V2 was set to 5-pass 6, 7-pass 8, 9-pass 10.
At this time, the first chromatographic pump P1 delivers the first mobile phase S1 to pass through the first flow channel first section L1, the first flow channel second section L2, the first flow channel third section L3, the first connecting line L4 and the waste flow channel L7, and the first mobile phase S1 is stopped to be delivered by the waste port W1 discharge device or the first chromatographic pump P1. The third chromatography pump P4 is suspended and the delivery of the third mobile phase S3 is stopped.
Meanwhile, the second chromatographic pump P2 delivers a second mobile phase S2 to pass through a second flow channel first section L8, a second flow channel second section L9 and a second connecting pipeline L5, and then to the first chromatographic column C1, so that the target is eluted from the first chromatographic column C1, passes through a third connecting pipeline L6 and an analysis flow channel first section L10 pipeline, and then passes through a second chromatographic column C2 to complete separation, and then passes through an analysis flow channel second section L11 mobile phase flow channel to enter a detector D to complete detection.
Claims (8)
1. A two-dimensional liquid chromatograph capable of reducing effects of a hplc matrix, comprising:
a first flow path for conveying a first mobile phase (S1);
a second flow path for conveying a second mobile phase (S2);
an analysis flow channel connected with a second chromatographic column (C2) for separating and detecting the captured substance;
a waste liquid flow passage (L7) for discharging waste liquid;
a first multi-channel switching valve (V1) and a second multi-channel switching valve (V2) connected to each other for connecting and switching the channels; the first multi-channel switching valve (V1) and the second multi-channel switching valve (V2) are provided with a plurality of ports;
it is characterized in that the AIR purifier also comprises an AIR flow passage connected with an AIR filter (AIR) and an AIR compression pump (P3); one port of the first multi-channel switching valve (V1) is provided with a plug (T1), and any two other ports are respectively connected with the first channel and the air channel; a first chromatographic column (C1) is connected between any two ports of the second multi-channel switching valve (V2); any three ports of the second multi-channel switching valve (V2) are respectively connected with a second channel, a waste liquid channel (L7) and an analysis channel.
2. The two-dimensional liquid chromatograph capable of reducing the effect of the substrate of the LC-MS of claim 1, wherein the first multi-channel switching valve (V1) has 4 ports, i.e., port 1, port 2, port 3, and port 4; the second multi-channel switching valve (V2) has 6 ports, port 5, port 6, port 7, port 8, port 9 and port 10; the first flow channel is connected with the port 1, the air flow channel is connected with the port 3, the port 2 is provided with a plug (T1), the port 4 is connected with the port 5, the flow channels of the port 7 and the port 10 are connected with a first chromatographic column (C1), the port 8 is connected with the analysis flow channel, and the port 9 is connected with the second flow channel.
3. The two-dimensional liquid chromatograph of claim 1, further comprising a third flow path for delivering a third mobile phase (S3), wherein the third flow path is connected to the first flow path.
4. The two-dimensional liquid chromatograph capable of reducing the effect of the LC-MS matrix according to any of claims 1-3, wherein the first flow channel is provided with a first chromatographic pump (P1) and a Sample Injector (SIL).
5. The two-dimensional liquid chromatograph capable of reducing the effect of the LC-MS matrix of claim 3, wherein the first flow channel is provided with a first chromatographic pump (P1) and a Sample Injector (SIL), and the third flow channel is connected to the first flow channel at a rear end of the sample injector.
6. The two-dimensional liquid chromatograph capable of reducing the effect of the LC-MS matrix according to any one of claims 1-3, wherein a second chromatographic pump (P2) is disposed on the second flow path.
7. The two-dimensional liquid chromatograph capable of reducing the effect of the matrix of the LC-MS of any of claims 1 to 3, wherein said analysis flow channel is connected to a detector (D1).
8. The two-dimensional liquid chromatograph of any of claims 1-3, wherein the air filter is a filter capable of filtering out particles with a diameter greater than 0.2 μm from compressed air.
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