CN211263103U - Cell suspension concentration measurement and liquid preparation device - Google Patents
Cell suspension concentration measurement and liquid preparation device Download PDFInfo
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- CN211263103U CN211263103U CN201922159591.8U CN201922159591U CN211263103U CN 211263103 U CN211263103 U CN 211263103U CN 201922159591 U CN201922159591 U CN 201922159591U CN 211263103 U CN211263103 U CN 211263103U
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Abstract
The utility model discloses a cell suspension concentration measurement and join in marriage liquid device, its characterized in that: comprises an MCU, a monochromatic light source, a cuvette, a photoelectric detector, a cassette, a light guide tube, a quantitative liquid taking device and a liquid taking rod; the device adopts a monochromatic LED as a light source, and directly uses a light guide tube with certain length and diameter to realize the function of a collimating lens without using the collimating lens to collimate the light source; no filter is required to filter out light of other wavelengths; as long as before using liquid-based thin-layer cytology pelleter to make the film, get a certain amount of samples to the cell suspension and add and measure in the cell cuvette, can estimate the concentration value of the cell suspension that awaits measuring well promptly, dilute the ratio according to given dilution ratio parameter, obtain the cell suspension after diluting, the sample of making under this dilution ratio concentration has good homogeneity, can very big improvement film-making quality, overcome empty piece, take off the piece, pile up and the film-making result difference is big, the problem that the uniformity is not good.
Description
Technical Field
The utility model belongs to biomedical instrument design field, concretely relates to cell suspension concentration measurement and join in marriage liquid device for improving liquid-based thin layer cytology system piece quality.
Background
Cervical cancer is the second largest malignancy in women, with the incidence of malignancy in the female reproductive tract leading to its first occurrence. In 2015, the number of new cases in China is 9.89 ten thousand, the number of cases of death is 3.05 ten thousand, and the onset of cervical cancer shows a trend of youthfulness. The traditional manual film preparation method for cervical cancer screening is mainly a cervical smear pap smear, has low detection rate of abnormal cervical cells, high false negative rate and low film preparation speed, and cannot meet the requirements of large-scale, quick and accurate diagnosis in modern clinic.
The liquid-based thin-layer cytology (TCT) slide is produced by catalysis, the main methods include centrifugal slide, membrane slide and sedimentation slide, and different slide production methods need different kinds of equipment. Among them, there is a TCT membrane slide machine of hologic company, which performs slide making by blowing cells attached to a TCT membrane tube onto a slide glass by negative pressure and positive pressure. The prepared specimen has the problems of large difference, poor uniformity and the like because the membrane is blocked due to impurities and the like in the sample. In addition, a sedimentation type flaking machine of BD company can automatically complete flake preparation and cytological staining, and the flaking background is clear and the cell morphology is intact. However, the pretreatment process is very complicated, the flaking time is long, the cells are naturally settled and stacked, and the cells are not flatly laid on one layer, which causes inconvenience for observation under a mirror.
TCT film-making compares in traditional film-making method, and it has solved the not enough that traditional film-making exists to a certain extent, has improved the film-making effect. However, since the cell concentration of the cell suspension sample is not monitored in the process of preparing the cell specimen, the prepared cell specimen has the problems of slide removal, stacking, large difference of different sample preparation results, poor uniformity and the like, and the factors can directly influence the manual microscopic examination of a doctor or cause the condition of erroneous machine interpretation, namely erroneous judgment, after a pathology scanner is used for scanning the slide. This reduces the detection rate, requires re-production and interpretation, and even makes secondary sampling possible, thereby increasing the inspection period and the possibility of missed and erroneous determinations.
The method generally used for cell counting includes a counter measurement method, so that the cell concentration can be calculated, and the method needs a cell counter and uses a microscope to observe and count, is complex in operation, takes a long time, and cannot perform rapid detection. The spectrophotometer for measuring concentration has complex instrument mechanism, high cost and large volume, is commonly used for nucleic acid quantification and colorimetric protein quantification, needs a computer for use, is inconvenient to operate, cannot continuously work for a long time, and cannot be applied to actual measurement in the field. In addition, there is a method of using laser as a light source for measurement, and the measurement object is mainly suspended particles, which cannot be used for measurement of transparent cells. The utility model entitled cell measurement device (grant publication No. CN 103543093B), which relates to flow cytometry, requires fluorescent labeling of cells, whereas the art requires that the cells to be measured are colorless transparent label-free cell suspensions, which cannot be used for measurement. In addition, the concentration is measured by a photoelectric colorimeter, which is a colorimetry method based on the color of a solution of a substance to be measured or a colored solution formed by adding a color-developing agent, and cannot be used for measuring the concentration of colorless and transparent cells.
Since it is necessary to avoid contamination of the specimen in practical use, it is impossible to use a device for measuring the cell concentration of the specimen by directly inserting the specimen into the specimen liquid, and the total amount of the specimen liquid is only about 6ml, and it is impossible to use a device for measuring which requires a large amount of the specimen of the cell suspension.
Therefore, a device for measuring the concentration of a cell suspension, which is convenient to use, high in reliability, rapid and accurate, is needed to solve the problems of preparation of a specimen, cell stacking, cell dropping and empty slide, so that the interpretation accuracy and the slide reading efficiency can be greatly improved.
SUMMERY OF THE UTILITY MODEL
At least one in defect or improvement demand more than prior art, the utility model provides a cell suspension concentration measurement and join in marriage liquid device for improve liquid-based thin layer cytology system piece quality. The device adopts a monochromatic LED as a light source, and directly uses a light guide tube with certain length and diameter to realize the function of a collimating lens without using the collimating lens to collimate the light source; no filter is required to filter out light of other wavelengths; the concentration value of the sample to be detected can be obtained by taking a certain amount of sample from the cell suspension liquid and adding the sample into a cuvette for measurement before the liquid-based thin-layer cytology pelleter is used for flaking.
In order to achieve the above object, according to one aspect of the present invention, there is provided a cell suspension concentration measuring and liquid preparing apparatus, comprising a MCU, a cell suspension concentration measuring apparatus, and a cell suspension liquid preparing apparatus;
the cell suspension concentration measuring device comprises a monochromatic light source, a cuvette, a photoelectric detector, a cassette and a light guide cylinder; the monochromatic light source, the cuvette, the photoelectric detector and the light guide cylinder are arranged in the cassette, the cuvette is arranged between the monochromatic light source and the photoelectric detector, and the two sides of the cuvette are respectively provided with the light guide cylinders which are opposite and are respectively arranged between the monochromatic light source and the photoelectric detector;
the cell suspension liquid preparation device comprises a quantitative liquid taking device and a liquid taking rod; the quantitative liquid taking device is connected with the liquid taking rod, and the liquid taking rod comprises a liquid taking rod of a liquid to be detected and a diluent taking rod;
and the MCU is respectively connected with the monochromatic light source, the photoelectric detector, the light guide cylinder and the quantitative liquid taking device in a control mode.
Preferably, the cell suspension concentration measuring device further comprises a light source driving device connected between the monochromatic light source and the MCU for maintaining the stability of the light intensity of the light source and adjusting the light intensity.
Preferably, the cell suspension concentration measuring device further comprises a signal amplifying device connected between the photodetector and the MCU, and configured to collect a weak current signal generated by the photodetector and convert the weak current signal into a voltage signal that can be directly collected.
Preferably, the cell suspension concentration measuring apparatus further comprises a cassette cover mounted to the cassette; when the cassette cover is opened, the cuvette is taken and placed from the cassette.
Preferably, the quantitative liquid taking device of the cell suspension liquid dispensing device is a microliter-level flow liquid transfer pump.
Preferably, the microliter-level flow liquid transfer pump is respectively connected with the liquid taking rod for the liquid to be detected and the liquid taking rod for the diluent through silicone tubes.
Preferably, the cell suspension concentration measuring and liquid preparing device further comprises a display screen connected with the MCU, and used for operation prompt and information display in the measuring and liquid preparing process, providing a user interaction interface and facilitating the use of an operator.
The above-described preferred features may be combined with each other as long as they do not conflict with each other.
Generally, through the utility model discloses above technical scheme who conceives compares with prior art, has following beneficial effect:
1. the cell suspension concentration measuring and liquid preparing device of the utility model improves the quality of liquid-based thin-layer cytology slides; the device adopts a monochromatic LED as a light source, and directly uses a light guide tube with certain length and diameter to realize the function of a collimating lens without using the collimating lens to collimate the light source; no filter is required to filter out light of other wavelengths; the concentration value of the sample to be detected can be obtained by taking a certain amount of sample from the cell suspension liquid and adding the sample into a cuvette for measurement before the liquid-based thin-layer cytology pelleter is used for flaking.
2. The utility model discloses a cell suspension concentration measurement and join in marriage liquid device can estimate the concentration value of the cell suspension that awaits measuring well, dilutes the ratio according to given dilution ratio parameter, obtains diluting back cell suspension, and the sample of making under this dilution ratio concentration has good homogeneity, and the quality of improvement film-making that can be very big has overcome empty piece, has taken off the piece, has piled up and the film-making result difference is big, the not good problem of uniformity.
3. The utility model discloses a cell suspension concentration measurement and join in marriage liquid device, the device are finally applied to liquid-based thin-layer cytology pelleter film-making in-process, have improved the film-making quality to improve the artifical microscopic examination of doctor read piece efficiency and machine and read the interpretation rate of accuracy of piece, especially the relevance ratio of cervical cancer cell.
4. The utility model discloses a cell suspension concentration measurement and join in marriage liquid device, if the cell suspension of measurement is the cell of same classification, then only need do once the calibration when using for the first time, alright direct use.
Drawings
Fig. 1 is a schematic view of the overall structure of a cell suspension concentration measuring and dispensing device according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Furthermore, the technical features mentioned in the embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other. The present invention will be described in further detail with reference to the following embodiments.
As a preferred embodiment of the present invention, as shown in fig. 1, the present invention provides a rapid and stable cell suspension concentration measurement and liquid preparation device, which comprises an MCU1, a light source driving device 2, a monochromatic light source 3 (for example, a monochromatic LED light source), a cuvette 4, a photodetector 5 (for example, a photodiode), a signal amplification device 6, a cassette 7, a cassette lid 8, a light guide tube 9, a display screen 10, a quantitative liquid taking device 11, a silicone tube 12, a liquid taking rod 13, and a button control area 14.
The MCU1 is used for maintaining the normal stable work and the data acquisition of whole system, adopts the power supply of 220V. The light source driving device 2 is connected with the MCU1 through a lead for ensuring the constant luminous intensity and the light intensity adjustment of the LED light source. The LED light source is used for providing a signal source for measurement, is fixed at the middle position of the left side of the cassette 7, and is connected with the light source driving device 2 through a lead. The cuvette 4 is used for containing a cell suspension sample to be detected and is placed in a groove in the middle of the cassette 7, so that the cuvette 4 is positioned in the middle between the LED light source and the photodiode. The photoelectric detector 5 is used for detecting the transmission light intensity of the light emitted by the receiving light source after the light penetrates through the cuvette and the suspension sample to be detected in the cuvette. The signal amplification device 6 is connected with the photoelectric detector through a signal wire and is used for collecting weak current signals generated by the photoelectric detector, converting the weak current signals into voltage signals which can be directly collected and amplifying the voltage signals; the MCU1 is connected with the signal amplification device 6 through a signal line and is used for collecting output voltage signals. The cassette 7 is used for installing and fixing a light source, a cuvette and a photoelectric detector to form the whole reaction generating device. The cassette cover 8 is mounted above the cassette in order to ensure that the entire reaction process is carried out in dark conditions. The light guide tube 9 is positioned on two sides of the cuvette 4, thereby isolating stray light to a certain extent and achieving the collimation function. The display screen 10 is used for operation prompt in the operation process of an operator and display of finally obtained solution concentration and dilution ratio parameters, provides a user interaction interface, and is convenient for the operator to use. The quantitative liquid taking device 11 is used for quantitatively taking the liquid to be measured and the diluent. The liquid taking rod 13 is connected with the quantitative liquid taking device 11 through a rubber tube 12, and the liquid taking rod 13 comprises a liquid taking rod of a liquid to be detected and a diluent taking rod. The button control area 14 is composed of a measuring button B0, a sample liquid taking and discharging button B1, and a diluent liquid taking and discharging button B2.
The utility model discloses use the LED light source, its is with low costs, longe-lived, simple structure, easy control use. In order to improve the precision of the measuring system, a light guide tube structure is designed in the cassette 7, the function of the light guide tube structure is equivalent to the function of collimating the light emitted by the LED light source, the parallel light is ensured to be incident into the solution in the cuvette 4, and the influence of the stray light on the measurement is reduced. In addition, the cuvette 4 has the advantages of higher transmissivity to near ultraviolet and visible light, increased transmitted light intensity and reduced energy loss, thereby increasing the concentration range of measurable cell suspension and improving the measurement accuracy. The used quantitative liquid taking device 11, such as a peristaltic pump, is a high-precision micro-flow liquid transferring pump, the precision can reach a microliter level, and the liquid can be accurately and quantitatively taken.
The utility model discloses a cell suspension concentration measurement and join in marriage liquid working method of device, including following step:
s1, measuring a cell suspension absorption spectrum curve through a spectrophotometer or a spectrometer to obtain the maximum absorption wavelength, adopting a monochromatic light source with the wavelength, carrying out primary calibration (see the following step S2), injecting a plurality of groups of cell suspension samples with different concentrations into a cuvette, sequentially measuring the transmission light intensity of light emitted by the monochromatic light source after the light penetrates through the cuvette and an internal cell suspension sample, and converting the transmission light intensity into absorbance so as to obtain a standard curve of the relation between the concentration and the absorbance;
s2, a working power supply of the whole system is turned on, after preheating is carried out for more than 5 minutes, the cassette cover 8 is uncovered, the empty cuvette 4 is placed in the cassette 7, the cassette cover 8 is covered, the measurement button B0 is pressed according to the prompt on the display screen 10, and the MCU carries out zero calibration before measurement;
s3, sucking 1.5ml of fully and uniformly mixed cell suspension to be measured by using a burette, slowly extruding the cell suspension to be measured in the burette by attaching a titration nozzle to the inner wall of an empty cuvette, avoiding the generation of bubbles and influencing the accuracy of the measurement result, covering a cassette cover 8 after the cell suspension is added, pressing a measurement button B0, measuring the transmission light intensity of the cell suspension to be measured, converting the transmission light intensity into absorbance, and substituting the absorbance into a standard curve of the relation between the concentration and the absorbance to obtain the concentration of the cell suspension to be measured;
s4, calculating and controlling the respective sampling amounts of the liquid taking rod of the liquid to be detected and the liquid taking rod of the diluent according to the measured concentration of the cell suspension liquid to be detected and the preset dilution ratio parameters, mixing and fully shaking up, wherein the mixing step specifically comprises the steps of connecting the liquid taking rod of the liquid to be detected with a disposable pipette tip, putting the pipette tip into the cell suspension liquid to be detected which is fully and uniformly mixed in the step S3, pressing a button B1 for taking and putting the sample liquid, stopping sampling after the MCU controls the calculated sampling amount and finishing taking the liquid by a display screen, putting the pipette tip into an empty test tube, pressing a button B1 for taking and putting the sample liquid again, discharging the sample liquid and putting the pipette tip after the sample liquid is completely discharged; similarly, the diluent taking and discharging button B2 is used for sucking the diluent with the calculated sampling amount by using a diluent taking rod, and the diluent is also put into the same test tube and fully shaken up; obtaining 4ml of diluted and mixed liquid for tabletting, and removing 2ml of the liquid for tabletting by using a tabletting machine;
s5, replacing the cuvette, repeating the steps S2-S4, and carrying out concentration measurement and solution preparation on the next cell suspension to be detected.
Through contrast test discovery, not use the utility model discloses concentration measurement and join in marriage the A liquid of liquid device have the cell to take off the piece, pile up and inhomogeneous condition, and B liquid cell density is on the high side, and C liquid has the inhomogeneous condition of picture, does not use the utility model discloses concentration measurement and join in marriage a single sample slide uniformity of making of liquid device are not good, and the difference nature is big between the different concentration sample liquid film-making result, and the uniformity is poor. And use the utility model discloses behind the concentration measurement and the liquid preparation device, single slide sample cell smear is more even, and difference is less between the different concentration sample liquid film-making results.
Through a plurality of experimental tests, the cell suspension concentration measuring and preparing device can well estimate the concentration value of the cell suspension to be measured, dilution proportioning is carried out according to given dilution proportioning parameters to obtain the diluted cell suspension, and a specimen prepared under the dilution proportioning concentration has good uniformity and can greatly improve the quality of the slide.
To sum up, the utility model has the following substantive features and is showing the progress:
the cell suspension concentration measuring and liquid preparing device of the utility model improves the quality of liquid-based thin-layer cytology slides; the device adopts a monochromatic LED as a light source, and directly uses a light guide tube with certain length and diameter to realize the function of a collimating lens without using the collimating lens to collimate the light source; no filter is required to filter out light of other wavelengths; the concentration value of the sample to be detected can be obtained by taking a certain amount of sample from the cell suspension liquid and adding the sample into a cuvette for measurement before the liquid-based thin-layer cytology pelleter is used for flaking.
The utility model discloses a cell suspension concentration measurement and join in marriage liquid device can estimate the concentration value of the cell suspension that awaits measuring well, dilutes the ratio according to given dilution ratio parameter, obtains diluting back cell suspension, and the sample of making under this dilution ratio concentration has good homogeneity, and the quality of improvement film-making that can be very big has overcome empty piece, has taken off the piece, has piled up and the film-making result difference is big, the not good problem of uniformity.
The utility model discloses a cell suspension concentration measurement and join in marriage liquid device, the device are finally applied to liquid-based thin-layer cytology pelleter film-making in-process, have improved the film-making quality to improve the artifical microscopic examination of doctor read piece efficiency and machine and read the interpretation rate of accuracy of piece, especially the relevance ratio of cervical cancer cell.
The utility model discloses a cell suspension concentration measurement and join in marriage liquid device, if the cell suspension of measurement is the cell of same classification, then only need set up the parameter of standard curve when first using, alright direct use.
It will be understood by those skilled in the art that the foregoing is merely a preferred embodiment of the present invention, and is not intended to limit the invention to the particular forms disclosed, but on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.
Claims (7)
1. A cell suspension concentration measurement and liquid preparation device is characterized in that: comprises an MCU, a cell suspension concentration measuring device and a cell suspension liquid preparation device;
the cell suspension concentration measuring device comprises a monochromatic light source, a cuvette, a photoelectric detector, a cassette and a light guide cylinder; the monochromatic light source, the cuvette, the photoelectric detector and the light guide cylinder are arranged in the cassette, the cuvette is arranged between the monochromatic light source and the photoelectric detector, and the two sides of the cuvette are respectively provided with the light guide cylinders which are opposite and are respectively arranged between the monochromatic light source and the photoelectric detector;
the cell suspension liquid preparation device comprises a quantitative liquid taking device and a liquid taking rod; the quantitative liquid taking device is connected with the liquid taking rod, and the liquid taking rod comprises a liquid taking rod of a liquid to be detected and a diluent taking rod;
and the MCU is respectively connected with the monochromatic light source, the photoelectric detector, the light guide cylinder and the quantitative liquid taking device in a control mode.
2. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:
the cell suspension concentration measuring device also comprises a light source driving device which is connected between the monochromatic light source and the MCU and is used for maintaining the stability of the light intensity of the light source and adjusting the light intensity.
3. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:
the cell suspension concentration measuring device also comprises a signal amplifying device which is connected between the photoelectric detector and the MCU and used for collecting weak current signals generated by the photoelectric detector and converting the weak current signals into voltage signals which can be directly collected.
4. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:
the cell suspension concentration measuring device further comprises a cassette cover mounted on the cassette; when the cassette cover is opened, the cuvette is taken and placed from the cassette.
5. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:
the quantitative liquid taking device of the cell suspension liquid preparation device is a microliter-level flow liquid transfer pump.
6. The cell suspension concentration measuring and dispensing apparatus of claim 5, wherein:
the microliter-level flow liquid-moving pump is respectively connected with the liquid-taking rod of the liquid to be detected and the diluent liquid-taking rod through silicone tubes.
7. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:
the cell suspension concentration measuring and liquid preparing device further comprises a display screen which is connected with the MCU and used for operation prompt and information display in the measuring and liquid preparing process, a user interaction interface is provided, and the use by an operator is facilitated.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201922159591.8U CN211263103U (en) | 2019-12-05 | 2019-12-05 | Cell suspension concentration measurement and liquid preparation device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201922159591.8U CN211263103U (en) | 2019-12-05 | 2019-12-05 | Cell suspension concentration measurement and liquid preparation device |
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| CN211263103U true CN211263103U (en) | 2020-08-14 |
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