CN101487788A - Multi-channel flowing counting pool - Google Patents
Multi-channel flowing counting pool Download PDFInfo
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- CN101487788A CN101487788A CNA2008100101064A CN200810010106A CN101487788A CN 101487788 A CN101487788 A CN 101487788A CN A2008100101064 A CNA2008100101064 A CN A2008100101064A CN 200810010106 A CN200810010106 A CN 200810010106A CN 101487788 A CN101487788 A CN 101487788A
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Abstract
The invention relates to a multi-channel running counting cell fixed on an object stage. The invention is characterized in that the multi-channel running counting cell has an integral spacer (3) structure, hollow counting chambers (1) are processed in the spacer (3), the counting chambers (1) are corresponding to the counter channels (2) located at the upper side and the lower side of the shafts of the counting chambers, and the quantity of the counting chambers is identical to that of the counter channels; and 2-100 counting chambers (1) are provided. With respect to the prior art, the invention has the following advantages that the counting cell has a plurality of channels, the spacers are made of titanium alloy materials and the shell is made of metal; the counting cell has better use stability, lower working pollution and higher use efficiency; and the counting cell has obviously foreseeable great economic and social benefits.
Description
Technical field:
The present invention relates to medical science and chemical analysis and experimental science, a kind of multi-channel flowing counting pool is provided especially.
Background technology:
1988, the U.S. developed the arena automatic analyzer of first high-speed camera formula in the world, this instrument be particle image show with sample on the screen of computing machine, differentiated by the reviewer.Nineteen ninety, Japan produces the urine sediments analyzer of imaging cytometer art, mainly form, comprise strobo, enlarging objective, plane flow cell, CCD camera chain, image information processor and cathode ray oscilloscope (CRO) etc. by the mobile site of continuous high speed camera chain.But it is former thereby fail to popularize than costliness etc. that because this type of arena automatic analyzer is very dissatisfied to image particle mapping, processing power is low, poor repeatability, cast are differentiated unclear, price.Nineteen ninety-five, 1996,1003, Japan, Germany, the U.S.'s different urine sediments analyzer that releases one after another, equipment are more and more advanced.See on the principle of not associating that urine sediments analyzer roughly divides two classes, a class is a flow cytometry; Another kind of is to carry out image analysing computer again by the direct microscopy of arena, draws corresponding techniques data and experimental result.
Flow cytometry is the principle design that the size of in various degree fluorescence intensity, forward scattering light intensity and electrical impedance is arranged according to each cell.Instrument is transformed into electric signal with light signals such as fluorescence, scattered lights, and various signals are analyzed, and obtains histogram (histogram) and scatter diagram (scattergram) that each urine specimen produces at last.By analyzing these figures, can distinguish each cell and draw the form of cells involved.Therefore this analytical approach, when test result is contentious, still selects direct microscopy to identify at last because the process opto-electronic conversion can cause distortion in various degree.
The image-type urine sediments analyzer mainly scans the sample counting pond by microscope, camera lens gained image dataization, and get a plurality of equilibrium criterions.The early stage cell counting count board (sample panel) that adopts can be placed 10 and through pre-service centrifuged sample tally be inserted truss, imports scanning platform automatically into, and instrument is autoscan just.The cleaning of plug-in type tally, use pretty troublesomely, it is many to handle the sample that data need, and the cycle is long, and in the last few years, usefulness single channel flow-type counting chamber replaced the plug-in type tally gradually.Along with the user is more and more higher to efficient and accuracy requirement, the single channel counting chamber can not meet the demands.
Therefore, design, development, production hyperchannel flow count device are the important innovation of urine sediments analyzer, and it will have good market outlook.
Summary of the invention:
The purpose of this invention is to provide a kind of novel flowing counting pool, especially hyperchannel, metal material partition, metal shell, stability in use is better, the multi-channel flowing counting pool littler, that service efficiency is higher is polluted in work.
A kind of multi-channel flowing counting pool of the present invention, described counting chamber is fixed on the objective table; It is characterized in that: described multi-channel flowing counting pool has whole partition 3 structures, processes the counting chamber 1 of hollow in partition 3, and axially the counter passage 2 of both sides is corresponding up and down with being positioned at it for counting chamber 1, and the quantity of the two is identical; The quantity of counting chamber is 2～100.Metal tube is connected with counting chamber 1 and forms the passage of liquid flow.
Specifically include the essentially identical following three kinds of structures of housing 7 and length and width size in the described multi-channel flowing counting pool: partition 3, cover glass 4, wave carrier piece 5; Has one-sided groove shape structure on the housing 7, wave carrier piece 5, partition 3 and cover glass 4 and be fixedly mounted on from inside to outside successively respectively in the groove shape structure on the housing 7.
In the preferred version:
Be positioned at the counting chamber 1 axial counter passage 2 of both sides up and down and be circumscribed with metal tube 6, itself and counter passage 2 constitute liquid flow paths jointly;
In addition, multi-channel flowing counting pool of the present invention also has one of following feature or its combination:
The diameter of metal tube 6 is 0.4～1.0mm;
The tradition flowing counting pool is single pass, so overall dimensions is less, and external fluid communication circuit is single, installs easily and puts; If a plurality of passages, then whole counting chamber volume is bigger, and external fluid connection circuit is many, and counter need steadily be fixed on the objective table, and is clear, reliable during with the assurance microscopic examination.
The present invention has specially designed one and can fix with objective table, again can with the housing 7 of hyperchannel counting chamber coupling.Counting chamber can be put into housing 7, and can be convenient, smooth with glue, stable adhesion together; Simultaneously, profile will guarantee not influence being connected and distribution of counting chamber passage 2 and extraneous liquid line; Also to reserve with threaded hole so that fixing usefulness around the housing 7; The material of housing 7 can adopt the stainless steel of certain resistance to corrosion, adopts titanium alloy then more suitable.
The flow count chamber that gets a clear view is to form with the partition 3 of the wave carrier piece 5 of high-quality optical glass processing and materials such as cover glass 4, titanium alloy; Counting chamber 1 is bright and clean smooth, does not have any slit, guarantees that image is bright, the transmittance height, and no refraction effect provides best sharpness.The partition 3 of past single channel flowing counting pool adopts optical glass or stainless steel.The optical glass partition is smooth, and is corrosion-resistant, but the unusual difficulty of processing, for the hyperchannel counting chamber of complexity, counting chamber is 1 bigger, the cavity is many, and processing can't reach precision; The stainless steel thin slice is good and cheap, and is easy to process, but its corrosion resistivity ability is limited, often uses the phenomenon that made counting chamber 1 pollution continue to use with regard to corrosion phenomena occurring in 2 months.In addition, the easy attached cell of stainless steel surfaces is difficult for cleaning, and influences measuring accuracy.
The partition 3 of titanium alloy material has splendid antibiont liquid corrosion ability, and biological microbody such as cell is difficult for adhering to, and simultaneously titanium alloy has the characteristic of metal again, processing, installs all easily, based on this, adopts the partition 3 of titanium alloy.The size of partition 3 is identical with microslide and cover glass, is convenient to bondingly, keeps precision.The wave carrier piece 5 of single channel counting chamber is generally rectangle, and laterally incline respectively has one perpendicular to the side, with the consistent side opening of wave carrier piece 5 length directions.Side opening one end connects metal tube, and the other end is communicated with counting chamber 1.The degree of depth of side opening is generally 4～5 times of its diameter, and slightly larger in diameter is convenient to bonding in metal tube 6 diameters.Cover glass 4 is generally thinner, and size and wave carrier piece 5, partition 3 are measure-alike.In the middle of partition 3 was placed on, the below of partition 3 was a wave carrier piece 5, and the top is a cover glass 4, and the three forms counting chamber 1 after bonding, was used for observing, checking, and saw accompanying drawing 1,2.Sample can import from the metal tube 6 of an end, and observed in counting chamber 1, counting is discharged from the metal tube 6 of the other end then, finishes count measurement one time.
Because single channel once can only pass through a kind of sample, finish test after, also will abundant washes clean, for the big user of test volume, single channel can't meet the demands.Increase the physical dimension of wave carrier piece 5, especially increase lateral length, at the many groups of horizontal rib processing side opening, form the passage of several groups of metal tube+side openings+counting chamber+side opening+metal tube in the both sides of wave carrier piece 5, each passage is independently of one another, mutually noninterfere, operation separately, thereby realize the ability that a plurality of samples are tested simultaneously, improve the test speed concrete structure greatly referring to accompanying drawing 1,2.
In terms of existing technologies, the present invention has following design feature: hyperchannel, metal material partition, metal shell, and its stability in use is better, the work pollution is littler, service efficiency is higher; Have significantly foreseeable huge economy and social value.
Description of drawings:
Fig. 1 is a two-pass flow counting chamber synoptic diagram front view;
Fig. 2 is the A-A cut-open view of Fig. 1.
Embodiment:
Embodiment 1
A kind of two-pass flow counting chamber, shown in accompanying drawing 1,2, described counting chamber is fixed on the objective table; It is characterized in that: described multi-channel flowing counting pool has whole partition 3 structures, processes the counting chamber 1 of hollow in partition 3, and axially the counter passage 2 of both sides is corresponding up and down with being positioned at it for counting chamber 1, and the quantity of the two is identical; The quantity of counting chamber is 2.Metal tube is connected with counting chamber 1 and forms the passage of liquid flow.
Specifically include the essentially identical following three kinds of structures of housing 7 and length and width size in the described multi-channel flowing counting pool: partition 3, cover glass 4, wave carrier piece 5; Has one-sided groove shape structure on the housing 7, wave carrier piece 5, partition 3 and cover glass 4 and be fixedly mounted on from inside to outside successively respectively in the groove shape structure on the housing 7.
In addition, described multi-channel flowing counting pool also has following feature: the diameter of metal tube 6 is 0.4mm; Partition 3 is pure titanium partition, and its thickness is 0.02mm; Housing 7, partition 3, cover glass 4, wave carrier piece 5 these length along counting chamber 1 axial direction are 30mm.
Present embodiment and embodiment 1 content are basic identical, and its difference is:
The quantity of the counting chamber 1 of multi-channel flowing counting pool is 3, and metal tube is connected with counting chamber 1 and forms the passage of liquid flow.
Long 42 millimeters of present embodiment utilization, wide 36 millimeters, thick 2 millimeters high-precision quartz material microslides 5; Long 42 millimeters, wide 36 millimeters, thick 0.2 millimeter high-precision quartz cover glass 4; The partition 3 of long 42 millimeters, wide 36 millimeters, thick 0.1 millimeter pure titanium material; Long 42 millimeters, wide 50 millimeters titanium alloy casing---housing 7,0.6 millimeter of six diameter, the metal tube 6 of long 14 millimeters titanium alloy materials forms a sealing counting chamber through corrosion-resistant gluing connecing, counting chamber can flow through liquid simultaneously at three passages, for the instrument and equipment analytical test.
Present embodiment and embodiment 2 contents are basic identical, and its difference is:
Described multi-channel flowing counting pool also has following feature: the diameter of metal tube 6 is 1.0mm; Partition 3 is pure titanium partition, and its thickness is 0.5mm; Housing 7, partition 3, cover glass 4, wave carrier piece 5 these length along counting chamber 1 axial direction are 60mm.
Present embodiment and embodiment 1 content are basic identical, and its difference is:
Multi-channel flowing counting pool, the quantity of counting chamber 1 are 4.
Present embodiment adopts long 48 millimeters, wide 46 millimeters, thick 4 millimeters optical quartz microslides 5; Long 48 millimeters, wide 46 millimeters, thick 0.5 millimeter optical quartz cover glass 4; The partition 3 of long 48 millimeters, wide 46 millimeters, thick 0.05 millimeter pure titanium material; Select long 48 millimeters, wide 58 millimeters stainless steel casing---housing 7 for use; Have 0.8 millimeter of eight diameter, long 16 steel pipes that do not become rusty form a sealing counting chamber 1 through corrosion-resistant gluing connecing, and counting chamber can flow through liquid simultaneously at four passages, for the instrument and equipment analytical test.
Present embodiment and embodiment 4 contents are basic identical, and its difference is:
Described multi-channel flowing counting pool also has one of following feature or its combination:
The diameter of metal tube 6 is 0.6mm; Partition 3 is pure titanium partition, and its thickness is 0.05mm; Housing 7, partition 3, cover glass 4, wave carrier piece 5 these length along counting chamber 1 axial direction are: 50mm.
Present embodiment and embodiment 1 content are basic identical, and its difference is:
Multi-channel flowing counting pool, the quantity of counting chamber are 200.
In addition, the described multi-channel flowing counting pool of present embodiment also has one of following feature or its combination: the diameter of metal tube 6 is 0.4～1.0mm; Partition 3 is pure titanium partition, and its thickness is 0.02～0.5mm; Housing 7, partition 3, cover glass 4, wave carrier piece 5 these length along counting chamber 1 axial direction are: 30～60mm.
Claims (6)
1, a kind of multi-channel flowing counting pool, described counting chamber is fixed on the objective table; It is characterized in that:
Described multi-channel flowing counting pool has whole partition (3) structure, processes the counting chamber (1) of hollow in partition (3), and axially the counter passage (2) of both sides is corresponding up and down with being positioned at it for counting chamber (1), and the quantity of the two is identical; The quantity of counting chamber 1 is 2～100.
2, according to the described multi-channel flowing counting pool of claim 1, it is characterized in that: described multi-channel flowing counting pool includes housing (7) and the essentially identical following three kinds of structures of length and width size: partition (3), cover glass (4), wave carrier piece (5); Have one-sided groove shape structure on the housing (7), wave carrier piece (5), partition (3) and cover glass (4) are fixedly mounted on respectively in the groove shape structure on the housing (7) successively from inside to outside.
3, according to the described multi-channel flowing counting pool of claim 2, it is characterized in that: the partition in the described multi-channel flowing counting pool (3), cover glass (4), wave carrier piece (5) are respectively with corrosion-resistant glue bond together and be fixed on the housing (7).
4, according to the described multi-channel flowing counting pool of claim 3, it is characterized in that: housing in the described multi-channel flowing counting pool (7) and partition (3) are the titanium alloy material.
5, according to the described multi-channel flowing counting pool of claim 3, it is characterized in that:
Be positioned at the axial counter passage (2) of both sides up and down of counting chamber (1) and be circumscribed with metal tube (6), itself and counter passage (2) constitute liquid flow path jointly.
6, according to the described multi-channel flowing counting pool of claim 5, it is characterized in that: described multi-channel flowing counting pool also has one of following feature or its combination:
The diameter of metal tube (6) is 0.4～1.0mm;
Partition (3) is pure titanium partition, and its thickness is 0.02～0.5mm;
Housing (7), partition (3), cover glass (4), this length along counting chamber (1) axial direction of wave carrier piece (5) are: 30～60mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100101064A CN101487788A (en) | 2008-01-16 | 2008-01-16 | Multi-channel flowing counting pool |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100101064A CN101487788A (en) | 2008-01-16 | 2008-01-16 | Multi-channel flowing counting pool |
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| CN101487788A true CN101487788A (en) | 2009-07-22 |
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| CNA2008100101064A Pending CN101487788A (en) | 2008-01-16 | 2008-01-16 | Multi-channel flowing counting pool |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103196815A (en) * | 2013-04-08 | 2013-07-10 | 桂林优利特医疗电子有限公司 | Urinary sediment counting chamber |
| CN106526158A (en) * | 2016-11-14 | 2017-03-22 | 宜兴市晶科光学仪器有限公司 | Triple counter |
| CN107063977A (en) * | 2017-04-23 | 2017-08-18 | 湖南乾康科技有限公司 | A kind of multichannel counting chamber |
| CN107287119A (en) * | 2017-08-23 | 2017-10-24 | 湖南开启时代生物科技有限责任公司 | A kind of cell culture counting device |
| CN112052931A (en) * | 2020-09-02 | 2020-12-08 | 中国环境科学研究院 | Plankton counting frame |
| CN113933227A (en) * | 2021-12-17 | 2022-01-14 | 睿克环境科技(中国)有限公司 | Multi-channel microscopic observation counting frame and clamping device with same |
-
2008
- 2008-01-16 CN CNA2008100101064A patent/CN101487788A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103196815A (en) * | 2013-04-08 | 2013-07-10 | 桂林优利特医疗电子有限公司 | Urinary sediment counting chamber |
| CN103196815B (en) * | 2013-04-08 | 2014-11-26 | 桂林优利特医疗电子有限公司 | Urinary sediment counting chamber |
| CN106526158A (en) * | 2016-11-14 | 2017-03-22 | 宜兴市晶科光学仪器有限公司 | Triple counter |
| CN107063977A (en) * | 2017-04-23 | 2017-08-18 | 湖南乾康科技有限公司 | A kind of multichannel counting chamber |
| CN107287119A (en) * | 2017-08-23 | 2017-10-24 | 湖南开启时代生物科技有限责任公司 | A kind of cell culture counting device |
| CN112052931A (en) * | 2020-09-02 | 2020-12-08 | 中国环境科学研究院 | Plankton counting frame |
| CN113933227A (en) * | 2021-12-17 | 2022-01-14 | 睿克环境科技(中国)有限公司 | Multi-channel microscopic observation counting frame and clamping device with same |
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Open date: 20090722 |