CN1727902A - Full-automatic urine analysis system - Google Patents

Full-automatic urine analysis system Download PDF

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CN1727902A
CN1727902A CN 03152971 CN03152971A CN1727902A CN 1727902 A CN1727902 A CN 1727902A CN 03152971 CN03152971 CN 03152971 CN 03152971 A CN03152971 A CN 03152971A CN 1727902 A CN1727902 A CN 1727902A
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urine
sediment
mixer
system
sample
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CN 03152971
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王晓波
章春志
杜洪涛
徐昌奎
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合肥新月电子技术有限责任公司
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Abstract

A fully automatic analysis system of urine consists of a computer, sediment microscopic examination device controlled by computer, flow cell counting device , dry chemical analyzing device and integrated piping system for providing sample solution. It works as carrying out dry chemical analyzing and / or flow cell counting detection on urine sample, producing standard urine test report if detection result is negative and recording sample number and detection result if result is positive, sending positive sample to sediment microscopic examination device for producing out urine test report .

Description

全自动尿液分析系统 Automatic urine analysis system

技术领域 FIELD

发明涉及一种医疗领域中用于对尿液进行检测分析的技术。 FIELD The invention relates to a medical technique for detecting urine analysis.

技术背景尿液分析是尿液外观、理学、化学、显微镜检查的综合分析,是临床检验三大常规之一,广泛应用于临床,对肾脏及泌尿系统疾病的诊断、治疗及疗效监测具有重要价值。 BACKGROUND urinalysis urine appearance, science, chemistry, comprehensive analysis of microscopic examination, clinical laboratory is one of three conventional, widely used in clinical diagnosis of kidney and urinary system diseases, treatment and monitoring of the efficacy of great value . 目前国内外医院已普遍使用尿液分析装置进行尿常规检测,仅能检测尿液中的某些生化成分,如蛋白质、糖等,而不能检测尿液中的有形成分,如红细胞、白细胞、管型、结晶等。 At home and abroad has been widely used hospital urinalysis urine detection apparatus can only detect some urine biochemical components, such as proteins, sugars, etc., can not be detected in urine physical component, such as erythrocytes, leukocytes, casts, crystals and the like. 而临床上检查尿液中的有形成分均采用显微镜下人工进行分析判别,称为尿沉渣镜检(Examination of urinarysediments)。 Clinical examination and urine formed elements are used for analysis under the microscope artificial discriminant, called urine sediment examination (Examination of urinarysediments).

而传统的尿沉渣镜检,由于缺乏标准化的装置器和检查手段,人为因素造成操作者之间、实验室之间缺乏可比性,结果的差异极大,不能客观地反映检测的真实情况,不利于尿沉渣分析进行质量控制,得出定性或估量的结果还往往使医生难以对患者在诊断过程中进行动态的观察,直接影响临床诊断价值。 The traditional urine sediment examination, due to the lack of standardization of equipment and inspection means, caused by human factors between the operator, the lack of comparability between laboratories, the great differences in the results, can not objectively reflect the real situation detection, not Urine analysis is conducive to quality control, measure or qualitative results obtained also tend to make it difficult for doctors to observe patients in the dynamics of the diagnostic process, a direct impact on clinical diagnostic value.

近年来随着中国临床检验标准分委会(CCCLS)与美国临床检验标准委员会(NCCLS)接轨,我国已经开始着手制定尿液分析标准化文件,以提高日常尿分析的准确性和可比性。 In recent years, as China's Sub-Committee for Clinical Laboratory Standards (CCCLS) and the US Committee for Clinical testing standards (NCCLS) standards, China has started to develop a standardized file urine analysis to improve the accuracy and comparability of routine urinalysis. 要做到尿分析过程标准化,首先要使操作规范化,包括尿液标本采集规范化、使用器材的规范化、检查方法的规范化和判断标准的规范化等。 To do urinalysis process standardization, the first to make the standardized operation, comprising a standardized urine sample collection, using standardized equipment, standardization and criterion of standardized inspection method and the like. 因此,提供一种尿液分析标准化、规范化的系统装备已迫在眉睫。 Therefore, to provide a urine analysis standardization of systems and equipment is imminent.

发明内容 SUMMARY

本发明就是要克服现有尿液分析技术中存在的诸多缺陷,提供一种自动化、标准化、高精度的尿液分析系统,结合并利用于化学检验、流式细胞检验和尿沉渣镜检的各自优势,对尿液进行快速、准确的实施分析。 The present invention is to overcome the many deficiencies of the prior art urine analysis is present, there is provided an automated, standardized, high-precision urine analysis system, used for binding and chemical testing, flow testing and urine sediment examination respective advantages of urine fast, accurate implementation analysis.

发明内容 SUMMARY

为实现上述目的,本发明是通过采用以下技术方案实现的:全自动尿液分析系统,其特征在于:系统包括一个计算机和由该计算机控制的沉渣镜检装置、流式细胞计数装置和干化学分析装置,本系统还包括一个用于控制并向上述装置提供样液的进给集成管路系统。 To achieve the above object, the present invention is achieved by the following technical solutions: automatic urine analysis system, comprising: a computer system comprising means for controlling sediment microscopy and by the computer, flow cytometry, and dry chemistry apparatus analysis means further comprises a system for controlling and said means to provide the sample solution feeding pipe system integration.

系统首先对尿样进行干化学分析和/或流式细胞计数的检测,并对检测结果予以判断,检测结果为阴性则直接生成标准的尿检报告;检测结果为阳性则装置记录样本号及检测结果;同一批次标本全部结束第一阶段的过筛检验后,由自动取样装置进行离心,将阳性标本送至沉渣镜检装置,继续尿沉渣检验分析并得出尿检报告。 The system first chemical analysis of the urine sample is dry and / or flow cytometry to detect, and to give the detection determination result, the detection result is directly generated negative urine standard reports; Sample tested positive number and the detection result of means ; same batch after sieving test specimens of all the first stage, performed by the automatic sampling device centrifugation, the sediment microscopy positive samples to the apparatus, and continue to draw urine sediment analysis test report.

所述的进给集成管路系统接受计算机的控制信号并完成向对应装置提供并输送正确模式标本,其中具体完成将原尿输送并喷洒到干化学分析装置的检验槽,将原尿输送并通过高压喷射到流式细胞计数装置的细胞检测通道,将样液高速离心后把0.2毫升沉渣、0.2毫升染色沉渣或倍量稀释沉渣输送到镜检流动计数池中,并完成进样管道的清洗。 Said feeding pipe system integrated computer and receiving a control signal to provide complete and correct conveying specimens to a corresponding apparatus, wherein the specific original complete urinary conveyed and sprayed into a dry chemical analysis test device slot, the original conveyed by the urine cells injected into the high-pressure detecting passage of the flow cytometry apparatus, the sample was centrifuged at high speed after the sediment 0.2 ml, 0.2 ml or stained sediment sediment times the amount of dilution flow delivered to the microscopic cell counting, and complete cleaning of the injection pipe.

系统计算机还连有结果显示设备、键盘/鼠标操作设备和结果输出设备。 The computer system is also connected with a result display device, a keyboard / mouse device and a result output device.

由上述技术方案可知,本发明所提供的全自动尿液分析系统是新一代尿液分析检测系统,其中采用了电学、光学、流体力学,利用计算机技术、电子技术、流式细胞技术、图象处理技术、单片机技术、步进电机细分技术、光刻掩膜技术,使用模糊控制原理,采用独特的动力传动系统,定量计数池,实现了装置器动作的自动化、智能化、定量化,以及显微镜动作的全自动化。 Seen from the above technical solutions, automatic urine analysis system of the present invention provides a new generation of urine analysis detection system which uses the electrical, optical, fluid dynamics, using computer technology, electronic technology, flow cytometry, image processing, microcontroller technology, the stepping motor subdivision technology, photolithography masking techniques using the principles of fuzzy control, a unique powertrain, counting cell quantitative, automated, intelligent, quantitative actuation means, and Fully automated microscope operation. 采用多媒体编程和数据库编程技术,实现了图像和数据库的有机结合,及大容量多媒体数据查询。 Multimedia programming and database programming technology, a combination of the image and the database, and query large-capacity multimedia data. 使用VERTMENU控件及视频采集,对图象进行处理。 Use VERTMENU control and video capture, the image processing. 采用中值滤波、轮廓提取、或值分隔等,提高了细胞分类识别的准确度、可信度。 Median filtering, contour extraction, partition, or the like values, improve the accuracy of cell classification, and reliability.

附图概述图1是本发明的原理框图;图2是本发明的结构原理图。 BRIEF DESCRIPTION FIG. 1 is a block diagram of the present invention; FIG. 2 is a block schematic diagram of the present invention.

以下结合附图1和检测过程对本发明的原理作进一步的说明。 In conjunction with the accompanying drawings and the following one pair of detection process of the principles of the present invention will be further described.

干化学装置原理:干式尿液分析装置是在单一化学试带法测定尿液各种成分的基础上发展起来的。 Dry chemical means principle: Dry urine analysis device is developed on the basis of a single measurement of various components of urine test strips on the method. 过去是利用肉眼观察试带颜色的变化,与标准板进行比较得出相应的数值。 Past observation with the naked eye a color change of the test strip, a standard plate with the corresponding value obtained comparison. 而干式尿液分析装置则是在微电脑的控制下利用球面积分析仪将试带反射回来的光聚集到光电检测器上,经其转换成电信号,再经电路和计算机处理来测定试带上的颜色的变化进行阶段定量分析。 Dry the urine analysis apparatus is then determined by the test with the use of circuits and computer processing light onto the photodetector ball area analyzer with a reflective back again under the control of the microcomputer, which is converted into electric signals by, quantitative phase analysis was performed on the color change. 由于干式尿液分析装置自动完成上述测量过程,因此可获得比肉眼更精确的测试结果。 Since the urine analysis apparatus automatically dry the above measurement process, and therefore provides a more accurate test results than the naked eye. 干式尿液分析装置常用的试纸条(带)是在一条数十厘米长,5mm宽的塑料条上每隔约2mm粘上一块5mm宽的四方形状的试纸块,每个试纸块对应一项测量指标,另外在试纸条上还有一块不参加反应的空白块——补偿块作为尿液本底颜色,以对有色尿液及仪器变化等所引起的误差进行补偿。 Commonly used test strip (tape) Dry urine analysis device is about 2mm a glue strip 5mm wide block of a rectangular shape on an every tens of centimeters long, 5mm wide plastic strips, each strip corresponding to a block term measure, in addition there is a strip of blank blocks does not participate in the reaction - compensation block urine as the background color, to compensate for the colored urine and errors caused by instrument changes. 测试时,将尿液浸泡后的试纸条放在仪器的测试架上,使其受到光电扫描机构的扫描。 Test, urine test strip after immersion in the test stand equipment, it is scanned photoelectric scanning mechanism. 光电检测机构上方是一个长条状的光源灯,光源灯发出的光可以通过球面积分的上下通光孔照射在试纸条的试剂块上,试剂块则会将一部分光反射到积分球内。 Photodetector means is above a elongated light source lamp, light emitted from the light source lamp may be on a reagent test strip block, block reagent will reflect light to pass through the aperture is irradiated vertically on the surface integral part of the integrating sphere. 由于试剂块颜色色及深浅不同,其反射的光强也不一样。 Due to the different colors and shades of color reagent cake, reflected light intensity is not the same. 例如,尿液中某种化学成分的含量较低,其相应的试剂块的颜色就较浅,它所反射的光强度;反之,若含量较高,则反射的光强度较弱。 For example, certain chemical components urine low content of the corresponding color on the reagent cake shallow, the intensity of light reflected from it; otherwise, if the content is higher, the intensity of the reflected light is weak.

球面积分仪具有会聚光线的作用,试剂块上所反射的光被球面积分仪会聚后通过滤光片照射到光电检测器上,光电检测器将光信号转换为电信号,电信号经放大器及数模转换送一到微处理器系统,微处理器求出被测的每种试剂块的反射率及补偿块的反射率并与事先存在微机中的反射率及表示分析成分浓度的曲线进行比较,最后由打印机输出半定量的等级符号及浓度值。 Spherical integrator has the effect of converging light, the light reflected on the reagent block is converged by the spherical integrator filter is irradiated onto the photodetector, the photodetector converts the optical signal into an electrical signal by the amplifier and the number of Get analog converter to the microprocessor system, the microprocessor obtains the measured reflectance and the reflectance of each reagent compensation block and the block is present in the microcomputer in advance the reflectance and constituent concentration curve represents the analysis are compared, semi-quantitative level of the final output symbols and the density value by the printer.

试剂块颜色的深浅除随各被测成分及含量的不同变外,还与尿液本身的颜色有关。 Block agents with different color shades other variations of each component to be measured and the content, but also with the color of the urine itself. 为消除这一误差,在试纸条上设有空白块(补偿块),它虽不随被测成分而发生颜色变化,但却随尿液的颜色变化。 To eliminate this error, it is provided on the test strip blank blocks (compensation block), though the color change which occurs with the component to be measured, but with urine color changes.

目前干式尿液分析装置采用双波长(通过滤光片的一束测定和一束参考光)测定法。 Currently the dry urine analysis device dual wavelength measurement (measured by a bundle of filter and one reference beam) method. 仪器可按下述公式自动计算出反射率R。 Instrument according to the following equation to automatically calculate the reflectance R.

试纸的反射率R试纸=Tm(试剂对测量波长的反射强度)/TS(试剂对参考波长的反射强度)×100%空白的反射率R空白=Cm(空白对测量波长的反射强度)/CS(空白对参考波长的反射强度)×100%总反射率RR=R试纸/R空白=Tm CS/TS Cm×100%流式细胞工作原理:流式细胞技术包括液流系统、光学系统、和电子系统。 Paper reflectance R dipstick = Tm (reagent reflection intensity measuring wavelength) / TS (reagent reflection intensity of a reference wavelength) × 100% white reflectance R white = Cm (white reflection intensity measuring wavelength) / CS (blank reference reflection intensity of the wavelength) × 100% total reflectivity strips RR = R / R blank = Tm CS / TS Cm × 100% flow operating principle: flow cytometry system comprising a flow, an optical system, and electronic system. 待测样本中的细胞经液流系统传送依序地通过流式细胞装置中激光照射的区域,细胞受激光的激发产生信号,被信号接收器接受并放大,这些放大了的信号经计算机分析处理,并以图表的形式直观地显示出来;流式细胞装置产生并分析的信号主要有光散射信号和萤光信号。 Signal analysis and processing by the computer system for transmitting the flow cell in the test sample by flow sequentially irradiated region of the laser apparatus, the excitation signal is generated by a laser cell, the amplified signal is accepted and a receiver, the amplified and graphically displayed visually; flow signal generating means and analyzing the main light scattering signal and fluorescence signal. 光散射信号的强弱可以反映细胞的大小、形态及胞浆颗粒化的程度等。 Intensity light scattering signal can reflect the size, shape and degree of cytoplasmic granules in the cells. 依萤光素的不同,用不同波长的光激发,发射出不同波长的萤光,可显示不同的颜色,这些萤光信号就可以反映不同的细胞生物学特性。 According to different luciferin, excitation with light of a different wavelength, emit fluorescence of different wavelengths, can display different colors, the fluorescent signal can reflect different biological characteristics of cells.

将待测细胞染色后制成单细胞悬液。 After staining test cell made of a single cell suspension. 用一定压力将待测样品压入流动室,不含细胞的平衡缓冲液在高压下从鞘液管喷出,鞘液管入口方向与待测样品流成一定角度,这样,鞘液就能够包绕着样品高速流动,组成一个圆形的流束,待测细胞在鞘液的包被下单行排列,依次通过激光照射区(液柱与入射的激光束垂直相交点称为检测区),被萤光染色的细胞受强烈的激光照射后发出各色萤光,同时产生光散射。 The sample to be tested with a certain pressure to flow into the pressure chamber, the equilibration buffer is ejected from the cell-free sheath fluid under high pressure pipe, the sheath liquid inlet pipe flow direction of the test sample at an angle so that it is possible to package the sheath liquid high velocity flow around the sample, consisting of a circular stream, sheath fluid in the test cell is the single row, followed by laser irradiation zone (liquid column of the incident laser beam perpendicularly intersecting point is called the detection zone), was cells stained fluorescent color emitted by the strong fluorescence after the laser irradiation, while the light scattering. 萤光信号的收集可在激光束垂直的90度方向放置光学系统(透镜、光栏、和滤片等)用以收集萤光信号,投向检测器。 Collecting fluorescent signals may be placed an optical system (lens, diaphragm, filter and the like) to collect fluorescence signals toward the detector 90 in the direction perpendicular to the laser beam. 萤光检测器是光电倍增管,散射光检测器是光电二极管,用来收集前向角散射光。 Fluorescence detectors are photomultiplier tubes, the scattered light detector is a photodiode, a scattered light collection angle used before. 光散射信号在前向小角(2-10度)进行深测,这种测量称“前向角散射”或“前方散射”。 Light scattering signal measured front to the deep small angle (2-10 degrees), this measurement called "forward scatter", or "forward scattering." 在这种情况下产生的光散射信号基本上反映了细胞体积的大小。 Light scattering signal in this case is produced substantially reflects the size of the cell volume. 细胞发出的萤光信号和光散射信号同时被呈90度角方向的萤光光电倍增管和前向光电二极管接收,被放大积分后转换为电子信号输入多道(1024道)脉冲分析装置,并在计算机屏幕上,以一维直方图、二维位图、或三维图形显示出来。 Fluorescent signals and light scattering signals are simultaneously emitted Cell 90 degree angle fluorescent photomultiplier tubes and photodiodes receiving forward, after being amplified integrated into an electronic multi-channel input signal (1024) pulse analysis means, and on a computer screen, a one-dimensional histogram, a two-dimensional bitmap, or three-dimensional graphic display. 或将图形和数据直接输入联机专用的计算机,或存入磁盘以备分析。 Or graphics data directly input line and a dedicated computer, or saved to disk for analysis. 计算机快速而精确地将所测数据计算出来,结合多参数分析,从而实现了细胞定量分析。 The computer quickly and accurately calculated from the measured data, in conjunction with multi-parameter analysis of cells in order to achieve a quantitative analysis.

层流原理:Re>2300为湍流Re≤2300为稳流Re=dρν/ηd:管径ρ:液体密度ν:液体流速η:黏滞系数Bemoulli定律:S1V1=S2V2S,V分别为管道的截面积和液体的流速沉渣镜检原理:通过微机控制实现尿沉渣标本和染色的定量提取及混合染色等一系列自动程序(对高密度标本自动倍量稀释)。 Layer-flow principle: Re> 2300 steady flow is turbulent Re≤2300 Re = dρν / ηd: diameter ρ: liquid density ν: liquid flow rate η: viscosity coefficient Bemoulli Law: S1V1 = S2V2S, V respectively the cross-sectional area of ​​the pipe liquid flow rate and sediment microscopy principle: the computer control to achieve quantitative extraction sediment specimens and staining and staining mixing a series of automatic procedures (the amount of auto-fold dilution of samples of high density). 将混合、染色后的标本输送至特制的光学沉渣定量流动计数池中,通过内置数码位相差显微镜摄像系统即可在显示器屏幕上获得清晰的彩色尿沉渣图像。 After mixing the specimen, dye delivered to the special optical counting cell sediment constant flow, urinary sediment to obtain a clear color image on a display screen by a phase contrast microscope imaging system built-in digital bits. 采用中值滤波、轮廓提取、或值分隔等,进行细胞分类识别,并对尿沉渣中有形成份进行定量计算。 Median filtering, contour extraction, or the like partition values ​​for cell classification, and urinary sediment formed element for quantitative calculation. 实现了尿液有形成分的定量分析。 To achieve a quantitative analysis of urine formed elements.

以上所述介绍了有关沉渣镜检装置、流式细胞计数装置和干化学分析装置各自的工作原理,而本发明的核心在于将它们有机地结合在一起。 For the above described apparatus sediment microscopy, flow cytometry and working principle of each dry chemistry analysis device, the core of the present invention is that they are organically combined. 首先沉渣镜检装置、流式细胞计数装置和干化学分析装置是各自独立的设备,都可以对尿液进行检测,并且得出对应的结论。 First means sediment microscopy, flow cytometric analysis of dry chemistry apparatus and devices is independent of the device, can be detected in urine, and derives the corresponding conclusions.

干化学检验速度非常快,可以达到20秒/标本,用它进行定性检测;流式细胞检验速度比较快,可以达到50秒/标本,能够准确地记录每微升尿液中的红细胞、白细胞含量,但它们都无法识别其它有形成分,两种检验都是一种过筛检测;而沉渣镜检装置完成的尿沉渣镜检是对尿液在镜下图象进行观测处理,利用摄像和计算机图象处理技术结合计算机自动识别技术,可以记录尿样中的各种有形成分,它的检测结果最全面,但它即使在充分利用各种图象技术的基础上,检测速度相比之下为最慢的一种,只能达到120秒/标本。 Dry chemical test very fast, up to 20 seconds / sample, it is used for qualitative detection; faster test flow, can reach 50 seconds / sample, it can be accurately recorded per microliter of the urine erythrocytes, leukocytes content , but they are unable to recognize other tangible components, sieving the two tests is a kind of detection; means the sediment microscopy examination is complete urine sediment observation in the endoscopic image processing using an imaging computer and computer image processing technology in conjunction with automatic identification technology, can be recorded in a tangible components in urine, it is the most comprehensive detection results, but even in full use of various imaging techniques based on detecting the speed comparison It is the slowest, only up to 120 seconds / sample. 本发明正是利用了上述各设备装置的各自优势,将其结合在一起。 The present invention is the use of the respective advantages of each of the equipment devices, join them. 本发明所公开的系统首先对尿样同时进行尿干化学和流式细胞的检测,并对得出的结果进行判断,判断的方法是利用计算机中的二进制数据,如果是阴性标本,就立刻生成一份标准的尿检报告,但若是阳性标本,装置会自动记录标本号及检测结果,等一次20个标本全部结束第一阶段的过筛检验后,由自动取样装置进行离心,并把阳性标本送到镜检装置,由该装置继续完成尿沉渣检验分析,并出具尿检报告。 The system first disclosed to simultaneously urine flow and urinary chemical testing, and the results for determination, the method determines that the binary data using a computer, if the sample is negative, is generated immediately a standard urine report, but if the sample is positive, the device will automatically record the sample number and the detection result, and the like all over the first 20 samples sieved test after the first stage, centrifuged by the automatic sampling device and the samples sent positive the examination device by the device continue to complete the analysis of urine sediment test, urine test and issue a report.

以下结合图2说明本发明中进给集成管路系统的结构和原理:所述的进给集成管路系统是由混合器16、泵、阀和管路构成的,其中样品液依次经由定量蠕动泵4、阀11与混合器16相连,染色液依次经由定量蠕动泵3、阀10与混合器16相连,稀释液依次经由定量蠕动泵2、阀9与混合器16相连,清洗液依次经由定量蠕动泵1、阀8与混合器16相连,混合器16的输出端分别与干化学分析装置的检验槽、流式细胞计数装置的细胞检测通道及镜检流动计数池连通。 The following description of the invention in conjunction with an integrated feeding system piping structure and principle Figure 2: Integration of the feed line system 16 by a mixer, pumps, valves and piping configuration, wherein the fluid sample sequentially through a peristaltic quantitative pump 4, a valve 11 is connected to the mixer 16, the quantitative staining solution sequentially through a peristaltic pump 3, the valve 10 is connected to the mixer 16, the diluent quantitative sequentially via a peristaltic pump 2, the valve 9 is connected to the mixer 16, the cleaning liquid sequentially through quantitative a peristaltic pump, the valve 8 is connected to the mixer 16, the output of mixer 16 are grooves with a dry chemical analysis test device, detecting cells by flow cytometry and microscopic flow passage means counting cell communication.

所述的进给集成管路系统中的混合器16依次经由阀15、高压泵7将原尿输送并喷洒到干化学分析装置的检验槽上,在高压泵7与干化学分析装置的检验槽之间设有压力传感器17,设置压力传感器17是对样液的压力加以控制,确保经由高压泵7的样液以雾状方式喷洒到试纸条上。 The integrated feed line system 16 sequentially through the mixer valve 15, the high pressure pump 7 urinary original conveyed and sprayed onto the dry chemical analysis test device grooves, the high pressure pump 7 and the inspection cups dry chemical analysis apparatus in It is provided between the pressure sensor 17, pressure sensor 17 is provided to control the pressure of the sample solution, to ensure that a mist is sprayed onto the strip manner via a high-pressure pump 7 of the sample solution.

所述的进给集成管路系统中的混合器16依次经由阀14、高压泵6将原尿喷射到流式细胞计数装置的细胞检测通道,在高压泵6与流式细胞计数装置的细胞检测通道之间设有液位传感器18,设置液位传感器18是用来精确控制一定体积的样液能够喷射到检测通道上。 The feed mixer 16 are sequentially integrated line system via the valve 14, the original high-pressure pump 6 cells injected into the urine detection channel flow cytometry apparatus, a high-pressure pump 6 detects cells in flow cytometry apparatus level sensor 18 is provided between the channels, the level sensor 18 is provided to accurately control the volume of sample solution can be injected into the detection channel.

所述的进给集成管路系统中的混合器16依次经由阀13、双向蠕动泵5将样液经高速离心后把0.2毫升沉渣、0.2毫升染色沉渣或倍量稀释沉渣输送到镜检流动计数池中。 The feed line system integrated mixer 16 via the valve 13 sequentially, bidirectional peristaltic pump the sample solution after the 5-speed centrifugation to sediment 0.2 ml, 0.2 ml dyeing amount of sediment or sludge delivered to fold dilution flow microscopic counting pool.

所述的进给集成管路系统中的混合器16经阀12接回废液收集容器,所述的流式细胞计数装置的细胞检测通道的废液和镜检流动计数池中废液经管道接回废液收集容器,就是说,检测完毕后,对管路、器件和混合器进行清洗,并用容器接受废液,设备清洗干净后以备下次使用。 The feed line system integrated mixer 16 through valve 12 back to the waste collection container, the waste liquid flow detection channel cell counting means and the flow of waste through line microscopic cell counting back to the waste liquid collecting container, that is, after testing of the pipeline, cleaning device and a mixer, and accepts the waste container, the device clean after the next use.

Claims (9)

  1. 1.一种全自动尿液分析系统,其特征在于:系统包括一个计算机和由该计算机控制的沉渣镜检装置、流式细胞计数装置和干化学分析装置,本系统还包括一个用于控制并向上述装置提供样液的进给集成管路系统和自动取样装置。 An automatic urine analysis system, characterized in that: the system comprises a computer and a sediment microscopy means controlled by the computer, and a dry chemical analysis apparatus flow cytometry apparatus, a system for controlling and further comprising providing to said sample liquid conduit means integrated feeding system and automatic sampling device.
  2. 2.根据权利要求1所述的全自动尿液分析系统,其特征在于:系统首先对尿样进行干化学分析和/或流式细胞计数的检测,并对检测结果予以判断,检测结果为阴性则直接生成标准的尿检报告;检测结果为阳性则装置记录样本号及检测结果;同一批次标本全部结束第一阶段的过筛检验后,由自动取样装置进行离心,将阳性标本送至沉渣镜检装置,继续尿沉渣检验分析并得出尿检报告。 2. Automatic urine analysis system according to claim 1, wherein: the system first chemical analysis of the urine sample is dry and / or flow cytometry to detect, and to give the detection determination result, the detection result is negative urine directly generate a standard report; test positive for the apparatus and a detection result sample number; same batch after sieving test specimens of all the first stage, was centrifuged by an automatic sampling device, the positive specimen to sediment mirror A biopsy apparatus, continue to test urine sediment analysis and urine test results report.
  3. 3.根据权利要求1或2所述的全自动尿液分析系统,其特征在于:所述的进给集成管路系统接受计算机的控制信号并完成向对应装置提供并输送正确模式标本,其中具体完成将原尿输送并喷洒到干化学分析装置的检验槽,将原尿输送并通过高压喷射到流式细胞计数装置的细胞检测通道,将样液高速离心后把0.2毫升沉渣、0.2毫升染色沉渣或倍量稀释沉渣输送到镜检流动计数池中,并完成进样管道的清洗。 The automatic urine analysis system according to claim 1, wherein: a feed line integrated computer system receiving the control signal and to provide complete and correct conveying specimens to a corresponding device, wherein in particular Urine complete original conveyed and sprayed into a dry chemical analysis test device slot, the original conveyed and sprayed into cells in urine detection channel flow cytometry apparatus by a high pressure, the sample was centrifuged at high speed after the sediment 0.2 ml, 0.2 ml staining sediment times the amount of sediment or dilution flow delivered to the microscopic cell counting, and complete cleaning of the injection pipe.
  4. 4.根据权利要求1或2所述的全自动尿液分析系统,其特征在于:计算机还连有结果显示设备、键盘/鼠标操作设备和结果输出设备。 4. The automatic urine analysis system according to claim 12, wherein: the computer is also connected with a result display device, a keyboard / mouse device and a result output device.
  5. 5.根据权利要求1或2所述的全自动尿液分析系统,其特征在于:所述的进给集成管路系统是由混合器(16)、泵、阀和管路构成的,其中样品液依次经由定量蠕动泵(4)、阀(11)与混合器(16)相连,染色液依次经由定量蠕动泵(3)、阀(10)与混合器(16)相连,稀释液依次经由定量蠕动泵(2)、阀(9)与混合器(16)相连,清洗液依次经由定量蠕动泵(1)、阀(8)与混合器(16)相连,混合器(16)的输出端分别与干化学分析装置的检验槽、流式细胞计数装置的细胞检测通道及镜检流动计数池连通。 The automatic urine analysis system according to claim 1, wherein: said feeding pipe system is integrated constituted by a mixer (16), pumps, valves and lines, wherein the sample washed successively with a mixer (16) is connected via a peristaltic pump quantitative (4), the valve (11), the quantitative staining solution via a peristaltic pump connected in sequence (3), the valve (10) and the mixer (16), sequentially through the diluent quantitative a peristaltic pump (2), the valve (9) (16) is connected to the mixer, successively connected to the cleaning liquid mixer (16) via a quantitative peristaltic pump (1), the valve (8), the output of the mixer (16), respectively groove dry chemical analysis and testing apparatus, flow cytometry and cell detection microscopic flow passage means counting cell communication.
  6. 6.根据权利要求5所述的全自动尿液分析系统,其特征在于:所述的进给集成管路系统中的混合器(16)依次经由阀(15)、高压泵(7)将原尿输送并喷洒到干化学分析装置的检验槽上,在高压泵(7)与干化学分析装置的检验槽之间设有压力传感器(17)。 6. The automatic urine analysis system as claimed in claim 5, wherein: said feed mixer (16) sequentially integrated pipe system via the valve (15), the high-pressure pump (7) the original Urine conveyed and sprayed onto the dry chemical analysis test device grooves, the high pressure pump is provided with a pressure sensor (17) between the groove and the dry chemical analysis test device (7).
  7. 7.根据权利要求5所述的全自动尿液分析系统,其特征在于:所述的进给集成管路系统中的混合器(16)依次经由阀(14)、高压泵(6)将原尿喷射到流式细胞计数装置的细胞检测通道,在高压泵(6)与流式细胞计数装置的细胞检测通道之间设有液位传感器(18)。 7. The automatic urine analysis system as claimed in claim 5, wherein: said feed mixer (16) sequentially integrated pipe system via the valve (14), the high-pressure pump (6) the original cells injected into the urine detection channel flow cytometry apparatus, provided with a level sensor (18) in the high-pressure pump (6) between the detection cell and the channel flow cytometry apparatus.
  8. 8.根据权利要求5所述的全自动尿液分析系统,其特征在于:所述的进给集成管路系统中的混合器(16)依次经由阀(13)、双向蠕动泵(5)将样液经高速离心后把0.2毫升沉渣、0.2毫升染色沉渣或倍量稀释沉渣输送到镜检流动计数池中。 8. The automatic urine analysis system according to claim 5, wherein: said feeding line system integrated mixer (16) via a sequence valve (13), a bidirectional peristaltic pump (5) after the sample was centrifuged at high speed to sediment 0.2 ml, 0.2 ml dyeing amount of sediment or sludge delivered to fold dilution flow microscopic cell counts.
  9. 9.根据权利要求5所述的全自动尿液分析系统,其特征在于:所述的进给集成管路系统中的混合器(16)经阀(12)接回废液收集容器,所述的流式细胞计数装置的细胞检测通道的废液和镜检流动计数池中废液经管道接回废液收集容器。 9. The automatic urine analysis system according to claim 5, wherein: said feeding line system integrated mixer (16) via a valve (12) back to the waste liquid collecting container, said a waste liquid flow detection channel cells and microscopic counting means counting cell flow back to waste via conduit waste collector container.
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US20110022327A1 (en) * 2009-07-24 2011-01-27 Mark Busenhart Urine work area manager for a urine work area
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CN101629946B (en) 2008-07-15 2014-04-09 彭艾 Urinary cell micro staining analysis method
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US9443058B2 (en) * 2009-07-24 2016-09-13 Roche Diagnostics Operations, Inc. Urine work area manager for a urine work area
US20110022327A1 (en) * 2009-07-24 2011-01-27 Mark Busenhart Urine work area manager for a urine work area
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CN102393705B (en) 2011-10-18 2013-12-18 爱威科技股份有限公司 Sample formed component analyzer automatic detection control apparatus and control method thereof
CN102393705A (en) * 2011-10-18 2012-03-28 长沙高新技术产业开发区爱威科技实业有限公司 Sample formed component analyzer automatic detection control apparatus and control method thereof
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