CN211112036U - Device for promoting cells to release vesicles - Google Patents
Device for promoting cells to release vesicles Download PDFInfo
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- CN211112036U CN211112036U CN201921107587.0U CN201921107587U CN211112036U CN 211112036 U CN211112036 U CN 211112036U CN 201921107587 U CN201921107587 U CN 201921107587U CN 211112036 U CN211112036 U CN 211112036U
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Abstract
The utility model is suitable for a cell technology field provides an equipment that promotes cell release vesicle, equipment includes: the cell suspension conveying unit is communicated and connected with the rotary spiral pipe and conveys the cell suspension to the rotary spiral pipe; the spiral pipe is rotated and takes a spiral shape; and the irradiation lamp is used for promoting the cells to release the vesicles and irradiating the cell suspension in the rotating spiral tube. Therefore, the utility model discloses a promote equipment of cell release vesicle can induce apoptosis for it is more convenient to promote the batch operation of cell release vesicle.
Description
Technical Field
The utility model relates to a cell processing technology field especially relates to an equipment that promotes cell release vesicle.
Background
A Chinese patent with the publication number of CN102302784A entitled "a tumor chemotherapy medicine preparation and a preparation method thereof" discloses a tumor chemotherapy medicine preparation and a preparation method thereof, wherein cell membrane fragments released by apoptosis are used as a carrier to wrap chemotherapy medicines, the cell membrane is phospholipid bilayer and has permeability, the spherical structure of the preparation can be maintained by centripetal pull force formed by protein fiber filaments called cytoskeleton in cells, under the condition of higher concentration of the chemotherapy medicines, the chemotherapy medicines enter the cells along concentration gradient to form uniform solute in the cells, when the cells are stimulated or apoptotic, part of the protein fiber filaments at the cell membrane attaching part of the cytoskeleton are broken or lose attachment, the centripetal pull force is suddenly disappeared, so that the local cell membrane structure expands outwards under the action of the centripetal pull force, protrudes and wraps the cell contents and is released to the outside in the form of vesicles to be sub-between the cells and molecules In the hierarchical structure, chemotherapy drugs in cells are wrapped, and the vesicles containing the chemotherapy drugs and with the diameter of 0.1-1 mu m are released. However, in the field of vesicle release by induced cells, an apoptosis inducer and a drug are often used to act on cells or ultraviolet rays are used to irradiate the cells to promote apoptosis and generate vesicles, but the apoptosis inducer and the drug introduce foreign substances which can affect the collection of target products in the later period, and the ultraviolet irradiation method is generally adopted to induce apoptosis.
The invention patent with application number CN102302784A discloses that the tumor chemotherapy pharmaceutical preparation is used for inducing cells to release vesicles through ultraviolet treatment, however, ultraviolet-induced apoptosis is in a laboratory stage, no special equipment is used for inducing apoptosis, and batch treatment is limited. Thus, the prior art techniques for promoting the release of vesicles from cells have significant limitations.
In view of the above, the existing techniques for inducing apoptosis obviously have inconvenience and disadvantages in practical use, so there is a need for improvement.
SUMMERY OF THE UTILITY MODEL
In view of the above-mentioned drawbacks, an object of the present invention is to provide a device for promoting cell to release vesicles, which provides a predetermined device to induce apoptosis, so that batch operation of promoting cell to release vesicles is more convenient.
In order to achieve the above object, the present invention provides an apparatus for promoting release of vesicles from cells, the apparatus comprising:
the cell suspension conveying unit is communicated and connected with the rotary spiral pipe and conveys the cell suspension to the rotary spiral pipe;
the spiral pipe is rotated and takes a spiral shape;
and the irradiation lamp is used for promoting the cells to release the vesicles and irradiating the cell suspension in the rotating spiral tube.
According to the device for promoting the cells to release the vesicles, the rotary spiral tube is horizontally arranged.
According to the device for promoting the cells to release the vesicles, the irradiation lamp is cylindrical;
a hollow cavity communicated with the middle part of the spiral rotary spiral pipe is formed in the middle part of the spiral rotary spiral pipe;
the irradiation lamp penetrates through the cavity of the rotary spiral tube.
The device for promoting the cells to release the vesicles further comprises a heat insulation sleeve, wherein the heat insulation sleeve is arranged in the cavity of the rotary spiral tube around the periphery of the irradiation lamp.
According to the device for promoting the cells to release the vesicles, a fixed support is erected between the rotary spiral tube and the heat insulation sleeve to support the heat insulation sleeve.
According to the device for promoting the release of the vesicles from the cells, the irradiation lamp is an ultraviolet irradiation lamp.
The apparatus for promoting release of vesicles from cells according to, the delivery cell unit comprising:
the liquid supplementing bottle is used for storing the cell suspension;
one end of the first hose extends into the liquid replenishing bottle, and the other end of the first hose is communicated with the rotary spiral pipe;
the first peristaltic pump is fixed on the first hose, and the first peristaltic pump works to convey the cell suspension in the liquid supplementing bottle to the rotary spiral tube through the first hose.
According to the device for promoting cells to release vesicles, a bottle cap is covered on the liquid supplement bottle, a first through hole and a second through hole are formed in the bottle cap, and the first hose penetrates through the first through hole and extends into the liquid supplement bottle; and the second through hole is provided with a sterile filter.
The apparatus for facilitating release of vesicles from cells according to, the apparatus further comprising:
the liquid storage bottle is used for storing the cell suspension irradiated by the irradiation lamp;
one end of the second hose extends into the liquid storage bottle, and the other end of the second hose is communicated with the rotary spiral pipe.
According to the device for promoting the cells to release the vesicles, the rotary spiral tube is made of quartz glass;
the heat insulation sleeve is made of quartz glass.
The utility model discloses a cell suspension's of being connected with rotatory spiral pipe intercommunication unit will the cell suspension carries extremely rotatory spiral pipe, it is right to promote the lamp that the cell released the vesicle in the rotatory spiral pipe the cell suspension shines, shines behind the predetermined time, makes the cell suspension can release the vesicle. Therefore, the utility model discloses a promote equipment of cell release vesicle can induce apoptosis for it is more convenient to promote the batch operation of cell release vesicle.
Drawings
Fig. 1 is a front view of an apparatus for promoting vesicle release from cells according to an embodiment of the present invention;
fig. 2 is a schematic structural view of a rotary spiral tube and a radiation lamp according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1-2, in a first embodiment of the present invention, there is provided an apparatus 100 for facilitating release of vesicles from cells, the apparatus 100 comprising:
a cell suspension conveying unit which is communicated and connected with the rotary spiral tube 10 and conveys the cell suspension to the rotary spiral tube 10;
the spiral tube 10 is rotated to be spiral;
a lamp 20 for promoting the release of vesicles from the cells irradiates the cell suspension in the spiral tube 10.
In this embodiment, the cell suspension is delivered to the spiral rotary tube 10 through a cell suspension delivery unit connected in communication with the spiral rotary tube 10, and then the cell suspension in the spiral rotary tube 10 is irradiated by using an irradiation lamp 20 for promoting the release of vesicles, for example, for 5 to 30 minutes, since the liquid of the cell suspension flows in the spiral rotary tube 10 in a rotating manner, all the cells of the cell suspension can be irradiated by the irradiation lamp 20, so that the cells in the cell suspension can be apoptotic, and the release of the vesicles is enabled. Preferably, the lamp 20 is an ultraviolet lamp 20. The spiral rotary tube 10 is preferably made of quartz glass, and can completely transmit the ultraviolet rays emitted by the ultraviolet irradiation lamp 20, so that the cell suspension in the spiral rotary tube 10 can more efficiently absorb the energy of the ultraviolet rays, the energy loss is reduced, the irradiation intensity is enhanced, the irradiation time is shortened, and the irradiation amount in unit time is increased.
Referring to fig. 1, in the second embodiment of the present invention, the rotary spiral tube 10 is horizontally disposed, and the deposition of cells in the cell suspension can be prevented by horizontally disposing the rotary spiral tube 10.
Referring to fig. 1 to 2, in a third embodiment of the present invention, the irradiation lamp 20 has a cylindrical shape;
a hollow cavity 50 communicated with the middle part of the spiral rotary spiral pipe 10;
the lamp 20 is disposed through the cavity 50 of the rotary solenoid 10.
In this embodiment, the spiral-shaped rotary spiral tube 10 has a hollow cavity 50 formed at the middle thereof, so that the irradiation lamp 20 can be disposed through the hollow cavity 50 of the spiral-shaped rotary spiral tube 10, thereby uniformly irradiating the cell suspension in the spiral-shaped rotary spiral tube 10.
Referring to fig. 1 to 2, in a fourth embodiment of the present invention, the apparatus 100 further comprises a heat insulation sleeve 30, the heat insulation sleeve 30 is disposed in the cavity 50 of the spiral rotary tube 10 around the periphery of the irradiation lamp 20, that is, the irradiation lamp 20 is disposed in the center of the spiral rotary tube through the heat insulation sleeve 30, the heat insulation sleeve 30 can insulate the heat of the ultraviolet irradiation lamp 20 to prevent the local temperature of the cell suspension from being too high, and the heat insulation sleeve 30 is preferably made of quartz glass and has no absorption to ultraviolet rays.
Referring to fig. 2, in the fifth embodiment of the present invention, a fixing bracket 40 is installed between the spiral rotary pipe 10 and the heat insulating sleeve 30 to support the heat insulating sleeve 30 and prevent the heat insulating sleeve 30 from directly contacting the spiral rotary pipe 10.
Referring to fig. 1, in a sixth embodiment of the present invention, the delivery cell unit includes:
a fluid supplement bottle 60 for storing the cell suspension;
a first hose 80, one end of which extends into the fluid supplement bottle 60, and the other end of which is connected with the rotary spiral tube 10;
a first peristaltic pump 70 fixed to the first flexible tube 80, wherein the first peristaltic pump 70 is operated to transfer the cell suspension in the fluid supplement bottle 60 to the spiral tube 10 through the first flexible tube 80.
In this embodiment, the cell suspension is stored in the solution bottle 60 in advance under a sterile environment; one end of a first hose 80 is extended into the fluid supplement bottle 60, the other end of the first hose is connected with the rotary spiral tube 10 in a communicating manner, a fluid inlet is formed in the rotary spiral tube 10, the first hose 80 is connected with the fluid inlet, and a first peristaltic pump 70 is fixed on the first hose 80, so that the cell suspension in the fluid supplement bottle 60 can be conveyed to the rotary spiral tube 10 through the first hose 80 when the first peristaltic pump 70 works.
Preferably, a bottle cap 90 is covered on the fluid supplement bottle 60, a first through hole 91 and a second through hole 92 are formed in the bottle cap 90, and the first hose 80 passes through the first through hole 91 and extends into the fluid supplement bottle 60; the second through hole 92 is provided with a sterile filter 93. The second through hole 92 allows the internal and external pressures of the fluid supplement bottle 60 to be the same, so that the flow rate of the cell suspension is uniform when the cell suspension is transferred to the spiral tube 10 through the first hose 80.
Referring to fig. 1, in a seventh embodiment of the present invention, the apparatus 100 further comprises:
a reservoir 110 for storing the cell suspension irradiated by the irradiation lamp 20;
one end of the second hose 120 extends into the liquid storage bottle 110, and the other end is connected to the rotary spiral tube 10.
In this embodiment, a liquid outlet is formed in the spiral rotary tube 10, one end of the second flexible tube 120 extends into the liquid storage bottle 110, and the other end of the second flexible tube 120 is connected to the liquid outlet of the spiral rotary tube 10, when the cell suspension in the spiral rotary tube 10 is irradiated by the irradiation lamp 20 for a predetermined time, the first peristaltic pump 70 is operated to deliver the cell suspension irradiated by the irradiation lamp 20 to the liquid storage bottle 110 through the second flexible tube 120.
To sum up, the utility model discloses a cell suspension's of being connected with rotatory spiral pipe intercommunication unit will cell suspension carries extremely rotatory spiral pipe promotes that the lamp of cell release vesicle is right in the rotatory spiral pipe cell suspension shines, shines behind the predetermined time, makes cell suspension can release the vesicle. Therefore, the utility model discloses a promote equipment of cell release vesicle can induce apoptosis for it is more convenient to promote the batch operation of cell release vesicle.
Naturally, the present invention can be embodied in many other forms without departing from the spirit or essential attributes thereof, and it should be understood that various changes and modifications can be made by one skilled in the art without departing from the spirit or essential attributes thereof, and it is intended that all such changes and modifications be considered as within the scope of the appended claims.
Claims (10)
1. An apparatus for facilitating the release of vesicles from a cell, the apparatus comprising:
the cell suspension conveying unit is communicated and connected with the rotary spiral pipe and conveys the cell suspension to the rotary spiral pipe;
the spiral pipe is rotated and takes a spiral shape;
and the irradiation lamp is used for promoting the cells to release the vesicles and irradiating the cell suspension in the rotating spiral tube.
2. The apparatus for facilitating release of vesicles from cells according to claim 1, wherein the rotating spiral tube is horizontally disposed.
3. The apparatus for promoting release of vesicles according to claim 2, wherein the irradiation lamp has a cylindrical shape;
a hollow cavity communicated with the middle part of the spiral rotary spiral pipe is formed in the middle part of the spiral rotary spiral pipe;
the illumination lamp is arranged through the cavity of the rotary spiral tube.
4. The apparatus for promoting release of vesicles according to claim 3, further comprising a heat insulating sleeve disposed around the outer circumference of the irradiation lamp in the cavity of the rotating spiral tube.
5. The apparatus for promoting release of vesicles according to claim 4, wherein a fixing support is erected between the rotating spiral tube and the heat insulation sleeve to support the heat insulation sleeve.
6. The apparatus for promoting release of vesicles according to claim 1, wherein the irradiation lamp is an ultraviolet irradiation lamp.
7. The apparatus for promoting release of vesicles from cells according to claim 1, wherein the delivery cell unit comprises:
the liquid supplementing bottle is used for storing the cell suspension;
one end of the first hose extends into the liquid replenishing bottle, and the other end of the first hose is communicated with the rotary spiral pipe;
the first peristaltic pump is fixed on the first hose, and the first peristaltic pump works to convey the cell suspension in the liquid supplementing bottle to the rotary spiral tube through the first hose.
8. The apparatus for promoting release of vesicles according to claim 7, wherein a bottle cap is disposed on the fluid bottle, a first through hole and a second through hole are disposed on the bottle cap, and the first hose passes through the first through hole and extends into the fluid bottle; and the second through hole is provided with a sterile filter.
9. The apparatus for promoting release of vesicles from cells according to claim 1, further comprising:
the liquid storage bottle is used for storing the cell suspension irradiated by the irradiation lamp;
one end of the second hose extends into the liquid storage bottle, and the other end of the second hose is communicated with the rotary spiral pipe.
10. The apparatus for promoting release of vesicles from cells according to claim 4, wherein the rotating spiral tube is made of quartz glass;
the heat insulation sleeve is made of quartz glass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921107587.0U CN211112036U (en) | 2019-07-16 | 2019-07-16 | Device for promoting cells to release vesicles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921107587.0U CN211112036U (en) | 2019-07-16 | 2019-07-16 | Device for promoting cells to release vesicles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211112036U true CN211112036U (en) | 2020-07-28 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921107587.0U Active CN211112036U (en) | 2019-07-16 | 2019-07-16 | Device for promoting cells to release vesicles |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211112036U (en) |
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2019
- 2019-07-16 CN CN201921107587.0U patent/CN211112036U/en active Active
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