CN211013766U

CN211013766U - FFPE sample preprocessing device

Info

Publication number: CN211013766U
Application number: CN201921942095.3U
Authority: CN
Inventors: 魏子涵; 王晓娜; 王艳茹; 李宏鑫
Original assignee: Shanghai Rols Biotech Co ltd
Current assignee: Shanghai Rols Biotech Co ltd
Priority date: 2019-11-11
Filing date: 2019-11-11
Publication date: 2020-07-14
Anticipated expiration: 2029-11-11

Abstract

The utility model discloses a FFPE sample preprocessing device relates to the dewaxing technical field of FFPE sample, including the preprocessing cavity, the storage cavity that communicate each other, separate the cavity, still include: the convex blocks are fixedly arranged at the two end parts of the separator; the clamping groove is formed in one side, close to the separating mechanism, of the pretreatment mechanism, and is formed in one side, close to the separating mechanism, of the storage mechanism; the fixing groove is communicated with the clamping groove; and telescopic partition boards arranged at two end sides of the partition mechanism along the height direction. The separation aspect between the water phase layer and the oil phase layer is arranged in the separation cavity, the separation cavity is completely separated from the pretreatment cavity and the storage cavity by arranging the telescopic separation plate, so that the water phase layer and the oil phase layer are completely separated, when a tester receives the device, the tester directly utilizes a pipette to absorb the solution in the storage cavity to test, the convenience is brought to a quick key, the interference of the oil phase layer is avoided, and the reliability of the test result is effectively improved.

Description

FFPE sample preprocessing device

Technical Field

The utility model relates to a dewaxed technical field of FFPE sample, more specifically says that it relates to an FFPE sample preprocessing device.

Background

Formalin-fixed paraffin embedding (FFPE) is a tissue preservation technique. This technique is used to maintain the nuclear protein structure by formalin fixation and then embedding the tissue in paraffin for histological diagnosis or study after sectioning. The method can fully maintain the original tissue morphology of the sample, can be stored for a long time and the like, and is widely applied to the fields of clinical pathological diagnosis, pathological mechanism research, cancer medication guidance and the like at present.

The traditional Chinese patent with publication number CN109142012A discloses a fast dewaxing device for an FFPE sample, and relates to the field of FFPE sample dewaxing. The FFPE sample rapid dewaxing device comprises a sample incubation chamber and a heating module, wherein the sample incubation chamber is connected with the heating module, the heating module is arranged to heat the sample incubation chamber to a specified temperature, and a closable opening is arranged above or on the side surface of the sample incubation chamber.

However, in the practical use process of the above-mentioned device, the amount of different layer solutions dissolved out from different samples may be different, and it cannot be guaranteed that the oil phase layer and the water phase layer are completely separated when the valve is started, if the amount of the water phase layer is less, the solution in the oil phase layer may enter the sample storage chamber, and the interference of the oil phase layer solution may be caused when the water phase layer solution is taken at a later time, which affects the inspection result, and needs to be improved urgently.

SUMMERY OF THE UTILITY MODEL

To this problem in the practical application, the utility model aims at providing an FFPE sample preprocessing device, the concrete scheme is as follows:

the utility model provides a FFPE sample preprocessing device, includes pretreatment mechanism, storage mechanism, partition mechanism, pretreatment mechanism is equipped with the preliminary treatment cavity, storage mechanism is equipped with the storage cavity, partition mechanism is equipped with the partition cavity, the preliminary treatment cavity the storage cavity, partition cavity communicate each other, just the partition cavity sets up the preliminary treatment cavity with between the storage cavity, still include: the convex blocks are fixedly arranged at two end parts of the separating mechanism along the height direction; the clamping groove is formed in one side, close to the separating mechanism, of the pretreatment mechanism, and is formed in one side, close to the separating mechanism, of the storage mechanism; the fixing groove is formed in one side, close to the separating mechanism, of the pretreatment mechanism, is formed in one side, close to the separating mechanism, of the storage mechanism, and is communicated with the clamping groove; clamping the lug in the clamping groove, rotating the lug to clamp the lug into the fixing groove so as to fix the connection between the pretreatment mechanism and the separation mechanism and fix the connection between the storage mechanism and the separation mechanism; the telescopic separation plates are arranged at two end sides of the separation mechanism in the height direction and are used for separating the pretreatment chamber from the separation chamber, and separating the storage chamber from the separation chamber; when the telescopic separation plate close to one side of the pretreatment mechanism is pulled out, the pretreatment chamber is separated from the separation chamber; when the telescopic partition plate near one side of the storage chamber is pulled out, the storage chamber is separated from the partition chamber.

Through above-mentioned technical scheme, set up the separation aspect between aqueous phase layer and the oil phase layer in dividing the cavity, the rethread sets up flexible division board, will divide cavity and preliminary treatment cavity, divide the cavity and separate the cavity and store the cavity completely and separate, makes aqueous phase layer and oil phase layer separate completely to what make to store in the storage cavity is pure aqueous phase layer, does not have remaining oil phase layer. When the tester receives the device, the storage mechanism is taken down, the liquid in the storage chamber can be directly absorbed by a pipette or a dropper for testing, the shortcut key is convenient, the interference of an oil phase layer is avoided, and the reliability of the test result is effectively improved.

Furthermore, the telescopic separation plate is arranged in the middle of the separation mechanism, telescopic rods are arranged on two sides of the telescopic separation plate in the length direction, and the telescopic rods penetrate through the separation mechanism and are communicated with the outer side of the separation mechanism; when the telescopic rod deviates from the telescopic partition plate to move, the telescopic partition plate seals the end face of the partition mechanism.

Through above-mentioned technical scheme, the experimenter of being convenient for pulls open flexible division board through the telescopic link in order to separate the preliminary treatment cavity with separate the cavity and, the storage cavity with separate the cavity, and when the experimenter pulled out the telescopic link, can utilize the telescopic link to form a rotation portion, and the experimenter rotates the telescopic link and can rotates separating mechanism, avoids directly holding separating mechanism lateral wall and rotates and produce the smooth phenomenon of hand.

Furthermore, one side of the telescopic rod, which is far away from the telescopic partition board, is provided with a hand-pulling part, and the cross-sectional area of the hand-pulling part is larger than that of the telescopic rod.

Through above-mentioned technical scheme, utilize hand power portion to be convenient for tester hand power telescopic link in order to drive the flexible division board and pull open, prevent to follow hand power portion length direction and follow the roll-off on hand power portion when tester hand power portion.

Furthermore, a heating mechanism is wrapped at the outer side wall of the pretreatment mechanism.

By the technical scheme, the dewaxing speed of the FFPE sample is increased, and the water phase layer and the oil phase layer are separated more completely.

Furthermore, the partition mechanism comprises a fixing rod, the fixing rod is fixedly arranged inside the side wall of the partition mechanism, and the end part of the fixing rod is fixedly connected with the bump.

Through the technical scheme, the connection between the fixed lug and the separation mechanism improves the overall stability of the separation mechanism.

Further, the separation mechanism comprises a fixing ring, the fixing ring is fixedly arranged inside the side wall of the separation mechanism, and the fixing rod penetrates through the fixing ring and is fixedly connected with the bump.

Further preferably, the protrusion and the fixing rod together form a 7-shape.

Further preferably, an end face of the partition mechanism is uniformly provided with four bumps along the circumferential direction of the partition mechanism.

Compared with the prior art, the beneficial effects of the utility model are as follows: through setting up the partition mechanism, set up the separation aspect between aqueous phase layer and the oil phase layer in dividing the cavity, the rethread sets up flexible division board, will divide cavity and preliminary treatment cavity, separate cavity and storage chamber and separate completely, makes aqueous phase layer and oil phase layer separate completely to what make storage chamber in save is pure aqueous phase layer, does not have remaining oil phase layer. When the device is received to the test personnel, take off storage mechanism, directly utilize pipette or burette to absorb the solution in the storage chamber, can obtain pure aqueous phase layer solution, alright test, make things convenient for the fast key, and avoided the interference of oil phase layer, effectively improve the test result credibility.

Drawings

FIG. 1 is a schematic sectional view of the apparatus taken along the height direction;

FIG. 2 is a schematic view of the separation mechanism as a whole;

fig. 3 is an overall view of the partition mechanism after the side wall is hidden.

Reference numerals: 1. a pretreatment mechanism; 2. a storage mechanism; 3. a separation mechanism; 4. a pre-processing chamber; 5. a storage chamber; 6. a separation chamber; 7. a bump; 8. a telescopic partition plate; 9. a telescopic rod; 10. a hand-pulling part; 11. a heating mechanism; 12. fixing the rod; 13. and (4) fixing the ring.

Detailed Description

The present invention will be described in further detail with reference to the following examples and drawings, but the embodiments of the present invention are not limited thereto.

As shown in fig. 1-3, an FFPE sample pretreatment device, including pretreatment mechanism 1, storage mechanism 2, partition mechanism 3, pretreatment mechanism 1 is equipped with pretreatment chamber 4, and storage mechanism 2 is equipped with storage chamber 5, and partition mechanism 3 is equipped with partition chamber 6, and pretreatment chamber 4, storage chamber 5, partition chamber 6 communicate each other, and partition chamber 6 sets up between pretreatment chamber 4 and storage chamber 5, still includes: the projections 7 are fixedly arranged at two end parts of the separating mechanism 3 along the height direction; the clamping groove is formed in one side, close to the separating mechanism 3, of the pretreatment mechanism 1, and is formed in one side, close to the separating mechanism 3, of the storage mechanism 2; the fixing groove is formed in one side, close to the separating mechanism 3, of the pretreatment mechanism 1 and one side, close to the separating mechanism 3, of the storage mechanism 2, and the fixing groove and the clamping groove are communicated with each other; the convex block 7 is clamped in the clamping groove, the convex block 7 is rotated to clamp the convex block 7 into the fixing groove, so that the connection between the pretreatment mechanism 1 and the separation mechanism 3 is fixed, and the connection between the storage mechanism 2 and the separation mechanism 3 is fixed; the telescopic partition plates 8 are arranged at two end sides of the partition mechanism 3 in the height direction and used for partitioning the pretreatment chamber 4, the partition chamber 6, the storage chamber 5 and the partition chamber 6; when the telescopic partition plate 8 close to one side of the pretreatment mechanism 1 is pulled out, the pretreatment chamber 4 is separated from the partition chamber 6; when the telescopic partition plate 8 near the side of the storage chamber 5 is pulled out, the storage chamber 5 is separated from the partition chamber 6.

Through above-mentioned technical scheme, set up the separation aspect between water phase layer and the oil phase layer in separating cavity 6, the rethread sets up flexible division board 8, will separate cavity 6 and pretreatment chamber 4, separate cavity 6 and storage chamber 5 and separate completely, make water phase layer and oil phase layer separate completely to what make storage cavity 5 in store is pure water phase layer, does not have remaining oil phase layer. When the tester receives the device, the storage mechanism 2 is taken down, the liquid in the storage chamber 5 can be directly absorbed by a pipette or a dropper for testing, the shortcut key is convenient, the interference of an oil phase layer is avoided, and the reliability of the test result is effectively improved.

Further, the telescopic partition plate 8 is arranged in the middle of the partition mechanism 3, telescopic rods 9 are arranged on two sides of the telescopic partition plate 8 in the length direction, and the telescopic rods 9 penetrate through the partition mechanism 3 and are communicated with the outer side of the partition mechanism 3; when the telescopic rod 9 deviates from the telescopic partition plate 8 to move, the telescopic partition plate 8 seals the end face of the partition mechanism 3.

Through the technical scheme, the tester can conveniently pull the telescopic partition plate 8 through the telescopic rod 9 to separate the pretreatment chamber 4 from the separation chamber 6 and the storage chamber 5 from the separation chamber 6, and when the tester pulls out the telescopic rod 9, the telescopic rod 9 can be utilized to form a rotating part, the tester can rotate the telescopic rod 9 to rotate the separation mechanism 3, and the phenomenon that the outer side wall of the separation mechanism 3 is directly held to rotate to generate hand slip is avoided.

Further, one side of the telescopic rod 9 departing from the telescopic partition plate 8 is provided with a hand-pulling part 10, and the sectional area of the hand-pulling part 10 is larger than that of the telescopic rod 9.

Through the technical scheme, the hand-pulling part 10 is used for facilitating a tester to pull the telescopic rod 9 to drive the telescopic partition plate 8 to be pulled open, and the tester is prevented from sliding out of the hand-pulling part 10 along the length direction of the hand-pulling part 10 when the tester pulls the hand-pulling part 10.

Further, the outer side wall of the pretreatment mechanism 1 is wrapped with a heating mechanism 11.

Furthermore, the partition mechanism 3 comprises a fixing rod 12, the fixing rod 12 is fixedly arranged inside the side wall of the partition mechanism 3, and the end part of the fixing rod 12 is fixedly connected with the lug 7.

Through above-mentioned technical scheme to the fixed lug 7 is connected with between the partition mechanism 3, improves partition mechanism 3 overall stability.

Further, the partition mechanism 3 comprises a fixing ring 13, the fixing ring 13 is fixedly arranged inside the side wall of the partition mechanism 3, and the fixing rod 12 penetrates through the fixing ring 13 and is fixedly connected with the bump 7.

Further preferably, the projection 7 and the fixing rod 12 together form a 7-shape.

Further preferably, an end surface of the partition mechanism 3 is uniformly provided with four projections 7 along the circumferential direction of the partition mechanism 3.

The utility model discloses the working process is as follows: the separation mechanism 3 is arranged between the pretreatment mechanism 1 and the storage mechanism, the projection 7 is clamped in the fixed groove to fix the connection of the pretreatment chamber 4, the separation chamber 6 and the storage chamber 5, the pretreatment chamber 4, the separation chamber 6 and the storage chamber 5 are communicated with each other to form a large chamber, the FFPE sample and the dewaxing liquid are placed in the large chamber, the heating mechanism 11 is started to heat the pretreatment chamber 4, the paraffin in the FFPE sample can be dissolved out from the dewaxing liquid, and the paraffin is insoluble in water, in the heating process, the nucleic acid sample enters an oil phase layer and is separated from a water phase layer, at the moment, the cracked nucleic acid sample in the water phase layer is obtained, and after dewaxing is completed, the storage mechanism is positioned at the bottom standing device for a period of time along the height direction, the telescopic rod 9 is stretched to pull out the telescopic partition plate 8, so that the pretreatment chamber 4, the partition chamber 6 and the storage chamber 5 are separated from each other, the storage mechanism is taken out, and the solution in the storage chamber 5 is directly sucked by a pipette or a dropper, whereby the test can be performed.

It is above only the utility model discloses a preferred embodiment, the utility model discloses a scope of protection does not only confine above-mentioned embodiment, the all belongs to the utility model discloses a technical scheme under the thinking all belongs to the utility model discloses a scope of protection. It should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims

1. The utility model provides a FFPE sample preprocessing device, includes pretreatment mechanism (1), stores mechanism (2), separating mechanism (3), pretreatment mechanism (1) is equipped with pretreatment chamber (4), store mechanism (2) and be equipped with storage chamber (5), separating mechanism (3) is equipped with separates cavity (6), pretreatment chamber (4), storage chamber (5), separate cavity (6) intercommunication each other, just separate cavity (6) and set up pretreatment chamber (4) with between the storage chamber (5), its characterized in that still includes:

the projections (7) are fixedly arranged at two end parts of the separating mechanism (3) along the height direction;

the clamping groove is formed in one side, close to the separating mechanism (3), of the pretreatment mechanism (1) and is formed in one side, close to the separating mechanism (3), of the storage mechanism (2);

the fixing groove is formed in one side, close to the separating mechanism (3), of the pretreatment mechanism (1), and is formed in one side, close to the separating mechanism (3), of the storage mechanism (2), and the fixing groove and the clamping groove are communicated with each other;

clamping the convex block (7) in the clamping groove, rotating the convex block (7) to clamp the convex block (7) in the fixing groove so as to fix the connection between the pretreatment mechanism (1) and the separation mechanism (3) and fix the connection between the storage mechanism (2) and the separation mechanism (3);

the telescopic separation plates (8) are arranged at two ends of the separation mechanism (3) in the height direction and are used for separating the pretreatment chamber (4) from the separation chamber (6), and separating the storage chamber (5) from the separation chamber (6);

when the telescopic separation plate (8) near one side of the pretreatment mechanism (1) is pulled out, the pretreatment chamber (4) is separated from the separation chamber (6);

when the telescopic partition plate (8) near the side of the storage chamber (5) is pulled out, the storage chamber (5) is separated from the partition chamber (6).

2. The FFPE sample pretreatment device according to claim 1, wherein the telescopic separation plate (8) is arranged in the middle of the separation mechanism (3), telescopic rods (9) are arranged along two sides of the telescopic separation plate (8) in the length direction, and the telescopic rods (9) penetrate through the separation mechanism (3) and are communicated with the outer side of the separation mechanism (3);

when the telescopic rod (9) deviates from the telescopic partition plate (8) to move, the telescopic partition plate (8) seals the end face of the partition mechanism (3).

3. The FFPE sample pretreatment device according to claim 2, wherein a hand-pulling part (10) is arranged on one side of the telescopic rod (9) which is far away from the telescopic separation plate (8),

the cross section area of the hand pulling part (10) is larger than that of the telescopic rod (9).

4. The FFPE sample pretreatment device according to claim 1, wherein a heating mechanism (11) is wrapped at the outer side wall of the pretreatment mechanism (1).

5. The FFPE sample pretreatment device according to claim 1, wherein the separation mechanism (3) comprises a fixing rod (12), the fixing rod (12) is fixedly arranged inside the side wall of the separation mechanism (3), and the end of the fixing rod (12) is fixedly connected with the lug (7).

6. The FFPE sample pretreatment device according to claim 5, wherein the separation mechanism (3) comprises a fixing ring (13), the fixing ring (13) is fixedly arranged inside the side wall of the separation mechanism (3), and the fixing rod (12) penetrates through the fixing ring (13) and is fixedly connected with the lug (7).

7. The FFPE sample pretreatment device of claim 6, wherein the bump (7) and the fixing bar (12) form a 7-shape together.

8. The FFPE sample pretreatment device according to claim 1, wherein an end face of the separation mechanism (3) is provided with four projections (7) uniformly along the circumferential direction of the separation mechanism (3).