CN206408215U - A kind of cell block embedding machine - Google Patents
A kind of cell block embedding machine Download PDFInfo
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- CN206408215U CN206408215U CN201720076785.XU CN201720076785U CN206408215U CN 206408215 U CN206408215 U CN 206408215U CN 201720076785 U CN201720076785 U CN 201720076785U CN 206408215 U CN206408215 U CN 206408215U
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- centrifuge
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Abstract
The utility model embodiment discloses a kind of cell block embedding machine, including the embedding component and centrifuge being made up of embedding base and centrifuge tube；The openend of centrifuge tube is detachably connected with the connecting portion on embedding base；The inner surface of centrifuge pot surrounds into centrifugal chamber, bottom surface inwall and sets primary heater；Centrifugation electric machine main shaft is stretched into centrifugal chamber and the mounting seat for centrifugal turntable to be fixedly mounted is fixedly installed in top；Centrifugal turntable includes that multiple circumferences are equally distributed, set the turntable base of non-penetrating fixing groove；There is inclination angle in the axis of fixing groove and centrifugal turntable.In cell block embedding process, centrifuge accurate temperature controlling can carry out multiple thermograde centrifugally operateds to cell block, it is ensured that cell block and the parcel of matrix, effectively prevent cell loss, improve cell block embedding quality；Moreover, removably being combined using embedding base and centrifuge tube, the collection of cell block can be once realized, cell block embedding efficiency is further improved.
Description
Technical field
The utility model is related to technical field of medical equipment, more particularly to a kind of cell block embedding machine.
Background technology
In clinical cytology pathology and scientific research field, cell block is subjected to FFPE, be carry out cytopathologic diagnosis, it is thin
The scientific research such as born of the same parents histochemistry and antibody screening and the important foundation of clinical position.
In order to prepare cell block, at present generally using the method for centrifugation, this method is entered first with test tube to few cells
Row is collected, and is centrifuged in centrifuge, is removed supernatant and is obtained few cells；Few cells are placed into after being carried out in embedded box
Continuous dehydration and FFPE flow, obtain cell block.Will using the method for matrix (such as egg, agar) in addition, also having
Cell encapsulation gets up the method for carrying out cell block preparation, but causes showing for the scattered separation of cell during matrix is wrapped up
As equally causing follow-up cell block to prepare of poor quality.
But utility model people by study find, in the above-mentioned methods, due to test tube assemble few cells not by
Matrix is wrapped up, and cell loss is easily lead in follow-up dehydration and FFPE flow, cell block prepares of poor quality.
Therefore, the preparation quality for how improving cell block is the technical problem of those skilled in the art's urgent need to resolve.
The content of the invention
A kind of cell block embedding machine is provided in the utility model embodiment, is prepared with solving cell block of the prior art
Ropy problem.
In order to solve the above-mentioned technical problem, the utility model embodiment discloses following technical scheme：
The utility model embodiment provides a kind of cell block embedding machine, including embedding component and centrifuge, wherein：
The centrifuge includes centrifuge pot, primary heater and centrifugation motor；The inner surface of the centrifuge pot
Surround into centrifugal chamber；First is set to add on the bottom surface inwall of the centrifuge pot, along the side inner walls of the centrifuge pot
Hot device；The centrifugation motor is arranged on the bottom of centrifuge and the electric machine main shaft of centrifugation motor passes through the centrifuge pot
Bottom surface is stretched into the centrifugal chamber；Mounting seat is fixedly installed at the top of the centrifugation electric machine main shaft, centrifugal turntable is fixedly mounted
In the mounting seat；
The centrifugal turntable includes the equally distributed turntable base of multiple circumferences；It is respectively provided with the side wall of each turntable base
There is a non-penetrating fixing groove, the axis of the fixing groove and centrifugal turntable has inclination angle, and the notch of the fixing groove is close
Electric machine main shaft；
The embedding component includes embedding base and centrifuge tube；First connecting portion is provided with the embedding base, institute
The openend and the connecting portion for stating centrifuge tube are detachably connected；The articulated end of the centrifuge tube is provided with the second connection
Portion, the second connecting portion matches with the fixing groove, and the embedding component is slidably hung on phase by second connecting portion
In 2 fixing grooves of the opposite flank of adjacent turntable base.
Alternatively, the embedding base includes setting fluted in box body, the box body；The side lateral wall of the box body
On be provided with the top of wedge-shaped platform, the wedge-shaped platform and be provided with the first neck；It is provided with and is mutually perpendicular on the bottom surface of the groove
Reinforcement, the reinforcement intersects at the center of groove floor；The position relative with first neck on groove bottom wall
The second neck of upper setting, second neck is located on reinforcement muscle line；The connecting portion is round table-like structure, and the connection
Portion is internally provided with the uniform cavity through round table-like structure, the bottom center of the connecting portion and the center superposition of groove；Institute
The internal diameter for stating connecting portion is less than or equal to the external diameter of centrifuge tube openend.
Alternatively, the connecting portion is connected with the matching of centrifuge tube openend screw thread.
Alternatively, the embedding component also includes the embedding lid matched with the embedding base, the embedding lid
A side on the first buckle matched with the first neck be set, set what is matched with the second neck on another side
Second buckle.
Alternatively, at the bottom land position of the fixing groove, along the direction horizontally disposed second that stretches out of hold-down support
Heater；Two relative secondary heaters of adjacent turntable base constitute a secondary heater group；Each described second adds
The hot separate temperature control of device group.
Alternatively, multiple fixing grooves being parallel to each other, adjacent fixing groove are provided with each side wall of the turntable base
Bottom land horizontal direction on spacing be more than or equal to centrifuge tube length, and the fixing groove length along away from centrifugation
Electric machine main shaft direction gradually increases.
Alternatively, the second connecting portion includes head rod and the second connection；The head rod and second connects
The axis of extension bar overlaps and vertical with the body of centrifuge tube；The end of the head rod and the second connecting rod
It can be respectively sleeved in corresponding fixing groove.
Alternatively, the head rod and the length of the second connecting rod are unequal.
A kind of cell block embedding machine provided from above technical scheme, the utility model embodiment, the embedding of cell block
Centrifuge described in machine includes centrifuge pot, primary heater and centrifugation motor；The inner surface of the centrifuge pot is surrounded into
Centrifugal chamber；Primary heater is set on the bottom surface inwall of the centrifuge pot, along the side inner walls of the centrifuge pot；Institute
State centrifugation motor and be arranged on the bottom of centrifuge and centrifuge the electric machine main shaft of motor and stretched into through the bottom surface of the centrifuge pot
In the centrifugal chamber；Mounting seat is fixedly installed at the top of the centrifugation electric machine main shaft, centrifugal turntable is fixedly mounted on the peace
Fill on seat；The centrifugal turntable includes the equally distributed turntable base of multiple circumferences；It is respectively provided with the side wall of each turntable base
There is a non-penetrating fixing groove, the axis of the fixing groove and centrifugal turntable has inclination angle, and the notch of the fixing groove is close
Electric machine main shaft.The embedding component includes embedding base and centrifuge tube；First connecting portion is provided with the embedding base, institute
The openend and the connecting portion for stating centrifuge tube are detachably connected；The articulated end of the centrifuge tube is provided with the second connection
Portion, the second connecting portion matches with the fixing groove, and the embedding component is slidably hung on phase by second connecting portion
In 2 fixing grooves of the opposite flank of adjacent turntable base., can be right by centrifuge accurate temperature controlling in cell block embedding process
Cell block carries out the centrifugally operated of multiple thermogrades, so as to ensure cell block and effective parcel of matrix, effectively prevents cell
Lose, and then improve cell block embedding quality；Moreover, removably combined by using embedding base and centrifuge tube, can
The collection of cell block is once realized, cell block embedding efficiency is further increased.
Brief description of the drawings
, below will be to embodiment in order to illustrate more clearly of the utility model embodiment or technical scheme of the prior art
Or the accompanying drawing used required in description of the prior art is briefly described, it should be apparent that, for ordinary skill people
For member, on the premise of not paying creative work, other accompanying drawings can also be obtained according to these accompanying drawings.
A kind of structural representation for embedding component that Fig. 1 provides for the utility model embodiment；
A kind of structural representation for embedding base that Fig. 2 implements to provide for the utility model；
A kind of structural representation for embedding lid that Fig. 3 provides for the utility model embodiment；
A kind of structural representation for centrifuge that Fig. 4 provides for the utility model embodiment；
A kind of structural representation for centrifugal turntable that Fig. 5 provides for the utility model embodiment；
A kind of structural representation for turntable base that Fig. 6 provides for the utility model embodiment；
Another centrifuge partial structural diagram that Fig. 7 provides for the utility model embodiment.
Embodiment
In order that those skilled in the art more fully understand the technical scheme in the utility model, below in conjunction with this reality
With the accompanying drawing in new embodiment, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that
Described embodiment is only a part of embodiment of the utility model, rather than whole embodiments.Based on the utility model
In embodiment, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example, should all belong to the scope of the utility model protection.
The utility model embodiment provides a kind of cell block embedding machine, and the cell block embedding machine includes the embedding He of component 1
Centrifuge 2.
Referring to Fig. 1, the structural representation of a kind of embedding component provided for the utility model embodiment, as shown in figure 1, institute
Stating embedding component 1 includes embedding base 11 and centrifuge tube 12.Be provided with connecting portion 117 on the embedding base 11, it is described from
The openend 121 of heart test tube is detachably connected with the connecting portion 117.
In the utility model embodiment, the centrifuge tube 12 includes the body 122 of both ends open, the body 122
Inside it is provided with the hollow cavity having through the body；The centrifuge tube openend 121 can be understood as the body
Any one end, the other end of the body 122 can be understood as the articulated end of centrifuge tube；The mounting of the centrifuge tube
Second connecting portion 123 is provided with end；In one exemplary embodiment, the second connecting portion 123 can be pipe cap shape structure,
The one-shot forming mode of second connecting portion 123 is realized to be fixedly connected with body 122, or the second connecting portion 123 can
Realized and be detachably connected with body 122 in the way of being connected through a screw thread；Is provided with the outer wall of the second connecting portion 123
One connecting rod 124 and the second connecting rod 125, in use, the embedding base 11 pass through first connecting portion 117 and centrifugation
Test tube 12 is connected, and the centrifuge tube 12 is further hung on centrifuge by second connecting portion 123, so that centrifugation
2 pairs of embedding components 1 of machine carry out centrifugally operated.Moreover, being additionally provided with graduation mark on the body 122, user is facilitated to use
Measured in journey.
Referring to Fig. 2, the structural representation for a kind of embedding base for implementing to provide for the utility model, as shown in Fig. 2 described
Embedding base 11 includes box body 111, wherein the box body 111 can be any one in rectangle, square and circle,
Do not limited in the utility model embodiment；Set fluted 112 in the box body 111, the shape of the groove 112 can be with
The shape of box body 111 is consistent or different, such as the described bit length quadrangle box of box body 111, and the groove 112 could be arranged to rectangular
Connected in star or circular groove etc..Multiple equally distributed cavernous structures are provided with the bottom surface of the groove 112；The box body
Wedge-shaped platform 113 is provided with 111 lateral wall, the top of the wedge-shaped platform 113 is provided with the first neck 114；It is described recessed
Be provided with orthogonal reinforcement 115 on the bottom surface of groove 112, the reinforcement 115 intersect at the bottom surface of groove 112 center,
Intensity for improving the embedding base 11, improves durability；It is relative with first neck 114 on the bottom wall of groove 112
Position on the second neck 116 is set, second neck 116 is located on reinforcement muscle line；The connecting portion 117 is round table-like
The uniform cavity of round table-like structure is provided through in structure, and the connecting portion 117 along the axis of the connecting portion 117,
The bottom center of the connecting portion 117 and the center superposition of the groove 112, the reinforcement 115 are only set in the specific implementation
Put in the square indentations 112 outside the connecting portion 117；The internal diameter of the connecting portion 117 is opened less than or equal to centrifuge tube
The external diameter at mouthful end 121, or the external diameter of the connecting portion 117 are more than or equal to the internal diameter of centrifuge tube openend 121, so exist
During specifically used, centrifuge tube openend 121 can be interference fitted with connecting portion 117, so realize centrifuge tube 12 with
Embed being detachably connected for base 11.The bottom surface of the connecting portion 117 constitutes hemi-closure space with side, so works as centrifuge tube
12 are connected to when being centrifuged on connecting portion 117, cell block can note be attached on the bottom surface of connecting portion 117.
In order to further strengthen the reliability that centrifuge tube 12 is connected with embedding base 11, and centrifuge tube 12 and embedding
The flexibility that base 11 is connected, in the utility model embodiment, the connecting portion 117 and the centrifuge tube openend 121
Screw thread matching connection.In one exemplary embodiment, internal thread, the centrifugation are additionally provided with the inwall of the connecting portion 117
External screw thread is additionally provided with the outer wall of test tube openend 121, or, external screw thread is provided with the outer wall of the connecting portion 117, institute
Be provided with internal thread on the inwall for stating centrifuge tube openend 121, by internal thread and it is externally threaded be mutually matched, realize centrifugation
Test tube openend 121 and the threaded connection of connecting portion 117, and then realize that centrifuge tube 12 connects with the detachable of embedding base 11
Connect.
Referring to Fig. 3, the structural representation of a kind of embedding lid provided for the utility model embodiment, as shown in figure 3, institute
It is lid-like to state embedding lid 13, and the size of the embedding lid 13 and the size of the embedding base 11 match, so that
The embedding lid 13 can cover the embedding base 11, so as to realize the sealing to embedding base 11.The embedding lid
The edge of the embedding base 11 is provided with the first frame 131, and size and the institute of first frame 131 on 13 bottom surface
The size for stating groove 112 matches, when embedding lid 13 is fastened into embedding base 12, makes the inwall of the embedding base 11
It can be in close contact with the outer wall of first frame 131；In first frame 131, on the bottom surface of the embedding lid 13
2 reinforcements and multiple equally distributed cavernous structures are provided with, the reinforcement is mutually perpendicular to and intersects at the embedded box
The center of lid 13；The center of the embedding lid 13 is additionally provided with the second frame 132, and second frame 132 is circular frame,
The center of second frame 132 and the center superposition of the embedding lid 13, the internal diameter of second frame 132 are more than or waited
External diameter in the top of connecting portion 117, when embedding lid 13 so being fastened into embedding base 12, the second frame 132 can
Sealing is realized to the cavity in connecting portion 117；First matched with the first neck 114 is set on one side of the lid
The second buckle 134 matched with the second neck 116 is set on buckle 133, another side, passes through the first neck 114 and the
One buckle 133, and the second neck 116 and the second buckle 134 cooperation snapping, realize embedding lid 13 and embedding base 11
It is detachably connected, the convenient cell block to being stored in embedding base 11 is preserved.
Referring to Fig. 4, the structural representation of a kind of centrifuge provided for the utility model embodiment, as shown in figure 4, described
Centrifuge 2 includes centrifuge pot 21, primary heater 22 and centrifugation motor 23；The inner surface of the centrifuge pot 21 is surrounded
Into centrifugal chamber 24；First is set on the bottom surface inwall of the centrifuge pot 21, along the side inner walls of the centrifuge pot 21
Heater 22；The centrifugation motor 23 is arranged on the bottom of centrifuge 2 and centrifuges the electric machine main shaft 231 of motor 23 described in
The bottom surface of centrifuge pot 21 is stretched into the centrifugal chamber 24；The top of the centrifugation electric machine main shaft 231 is fixedly installed installation
Seat 25, centrifugal turntable 26 is fixedly mounted in the mounting seat 25.In the specific implementation, the centrifuge pot 21 can be circle
Column pot, the primary heater 22 can be discoid heater.
Participation Fig. 5, a kind of structural representation of the centrifugal turntable provided for the utility model embodiment, referring also to Fig. 6,
A kind of centrifugal turntable partial structural diagram provided for the utility model embodiment, the centrifugal turntable 26 include multiple edges from
The circumferential equally distributed turntable base 261 of heart rotating disk 26；Non-penetrating fixation is provided with the side wall of each turntable base 261
Groove 262；There is angle in the axis of the fixing groove 262 and centrifugal turntable 26, and the fixing groove 262 notch close to motor
Main shaft 231, by setting inclined fixing groove, when centrifuge is centrifuged to embedding component, the effect of centrifugal force is enabled to
The bottom land that component is in fixing groove all the time is embedded, prevents embedding component from slipping, and then ensure being smoothed out for centrifugally operated；It is described
Embed component 1 and pass through the connecting rod 125 of head rod 124 and second and 2 fixing grooves on adjacent discs bearing opposite flank
262 connections, can also easily realize the disengaging with the centrifugal turntable 26.It should be noted that the fixing groove 262
Notch can be understood as along centrifugation electric machine main shaft 231 bearing of trend, close to the upper surface of turntable base 261 end, it is described
The notch of fixing groove 262 can be understood as the bearing of trend along centrifugation motor 231, the end away from the upper surface of turntable base 261.
In one exemplary embodiment, the centrifugal turntable 26 includes 6 equally distributed turntable bases 261, described turn
Disk bearing 261 is the bearing of sector structure；6 screws are additionally provided with the centrifugal turntable 26, pass through bolt and mounting seat 25
It is fixed, and rotated under the drive of centrifugation electric machine main shaft 231.Certainly in the specific implementation, the quantity of the turntable base 261 is simultaneously
The quantity in above-described embodiment is not limited to, those skilled in the art can set any number of turntable bases according to centrifugation demand
261, do not limited in the utility model embodiment.
In use, the openend of centrifuge tube 12 is fixed on to the first connecting portion 117 of embedding base 11 first
On, centrifuge tube 12 is assembled with embedding base 11；Then, the head rod 124 and second in second connecting portion 123
The end of connecting rod 125 is respectively sleeved in two adjacent fixing grooves 262, wherein described two adjacent fixing grooves 262 can
With the fixing groove 262 being interpreted as on two relative sides of adjacent turntable base 261, so, pass through second connecting portion 123
The connecting rod 125 of head rod 124 and second realizes being detachably connected for embedding component 1 and centrifuge 2.Moreover, described
One connecting rod 124 overlaps and vertical with the body 122 of centrifuge tube 12 with the axis of the second connecting rod 125；Described
The spacing of the end of one connecting rod 124 and the second connecting rod 125 is equal to fixing groove 262 on the opposite flank of adjacent discs bearing 261
Groove face spacing；In the utility model embodiment, the length of the head rod 124 and the second connecting rod 125 can phase
Together, i.e., head rod 124 is symmetrical arranged with the second connecting rod 125 relative to the axis of centrifuge tube 12.
Referring to Fig. 6, another centrifuge partial structural diagram provided for the utility model embodiment, as shown in fig. 6,
On the side wall of the turntable base 26, at the bottom land position of the fixing groove 262, along the side of stretching out of hold-down support 261
To horizontally disposed secondary heater 27.Two relative secondary heaters 27 of adjacent turntable base 261 constitute one second and added
Hot device group；Each separate temperature control of secondary heater group, can set for each secondary heater group in the specific implementation
Corresponding PLC temperature conditioning units are put, so as to realize independent temperature control.In centrifugally operated, the centrifuge tube 12 in embedding component 1 is understood into
Horizontality, it is at bottom land position and horizontally arranged by the way that secondary heater 27 is arranged on, then it ensure that the second heating
Device 27 can effectively be heated along the body of centrifuge tube 12 to the cell block of its inside and the mixed liquor of matrix, and then accurately
Temperature control.By setting secondary heater 27, and the secondary heater group that the secondary heater 27 is constituted, carry out high temperature from
During the heart, further the cell block and the mixed liquor of matrix in corresponding centrifuge tube 12 and embedding base 11 can be entered
Row heating, by finely tuning the temperature of secondary heater group, realizes the accurate control to temperature；Moreover, in the specific implementation, due to
Different secondary heater group independence temperature controls, can in same centrifuge, while carry out different temperatures gradient centrifugation, from
And further increase the embedding quality of cell block embedding machine and the flexibility of embedding operation.
During centrifugally operated, it may be necessary to while being centrifuged to multiple cell blocks, in order to improve the embedding of cell block
The practicality of machine, under the first performance, referring to Fig. 7, another turntable base provided for the utility model embodiment
Structural representation, in the utility model embodiment, multiple phases can be provided with each side wall of the turntable base 261
Spacing in mutual parallel fixing groove 262, the bottom land horizontal direction of adjacent fixing groove is more than or equal to the length of centrifuge tube, and
And the length of the fixing groove gradually increases along away from centrifugation electric machine main shaft direction.As shown in fig. 7, in an exemplary embodiment
In, it is provided with 2 fixing grooves being parallel to each other 262, i.e. the first fixing groove and second on each side wall of the turntable base 261
Fixing groove；First fixing groove is away from centrifugation electric machine main shaft 231, and the second fixing groove is close to centrifugation electric machine main shaft 231, the first fixing groove
Length L1 be more than the second fixing groove L2 length；Moreover, in the bottom land horizontal direction of the first fixing groove and the second fixing groove
Space D is more than the length of centrifuge tube 12；It is horizontally disposed on the outside of the bottom position of the first fixing groove to have one second
Heater, it is horizontally disposed on the outside of the bottom position of the second fixing groove to have another secondary heater, wherein fixing groove
Bottom position on the outside of, it can be understood as through the vertical extension in axis of bottom land and the centrifugation electric machine main shaft 231 of fixing groove
On line and principle centrifuge electric machine main shaft 231 position.
Under second of performance, on the basis of the embodiment shown in Fig. 7, in the side wall of each turntable base 261
On fixing groove 262 could be arranged to equal length, by set the connecting rod 125 of head rod 124 and second be different length
Degree, is centrifuged while realizing multiple embedding component 11.The length of head rod 124 can be for example set to be more than the second connecting rod
125 length, so in a pair of adjacent turntable bases 261, by the head rod of the embedding component 11 of centrifugation simultaneously
124 are arranged on the side wall of different turntable bases 261, it is possible to achieve embedding component 11 is wrong in the circumference of centrifugal turntable 26
Open, it is ensured that centrifuged while multiple embedding component 11.Moreover, the need for according to centrifugally operated, multiple different lengths can be set
The connecting rod 125 of head rod 124 and second combination, meet it is any number of embedding component 11 while centrifuge.
So, when multiple embedding bases 11 and corresponding centrifuge tube 12 are installed at the same time, each embedding He of base 11
Centrifugal pipe 12 can with it is interlaced, be independent of each other, can either meet it is multiple embedding components centrifuge simultaneously, can effectively save again
Space.
The cell block embedding machine provided from the description of above-described embodiment, the utility model embodiment, including embedding group
Part and centrifuge；Wherein, the embedding component includes embedding base and centrifuge tube；Connection is provided with the embedding base
Portion, the centrifuge tube openend is detachably connected with the connecting portion；The centrifuge includes centrifuge pot, the first heating
Device and centrifugation motor；The inner surface of the centrifuge pot surrounds into centrifugal chamber；On the bottom surface inwall of the centrifuge pot, edge
The side inner walls of the centrifuge pot set primary heater；The centrifugation motor is arranged on bottom and the centrifugation of centrifuge
The electric machine main shaft of motor is stretched into the centrifugal chamber through the bottom surface of the centrifuge pot；The top of the centrifugation electric machine main shaft
Mounting seat is fixedly installed, centrifugal turntable is fixedly mounted in the mounting seat；The centrifugal turntable includes multiple along centrifugation turn
The circumferentially equally distributed turntable base of disk；Non-penetrating fixing groove is provided with the side wall of each turntable base；The fixation
There is inclination angle in the axis of groove and centrifugal turntable, and the fixing groove notch close to electric machine main shaft；The embedding component includes
Embed base and centrifuge tube；Be provided with first connecting portion on the embedding base, the openend of the centrifuge tube with it is described
Connecting portion is detachably connected；The articulated end of the centrifuge tube is provided with second connecting portion, the second connecting portion with it is described solid
Determine groove to match, the embedding component is slidably hung on 2 of the opposite flank of adjacent discs bearing by second connecting portion
In fixing groove.In cell block embedding process, multiple thermogrades can be carried out to cell block by centrifuge accurate temperature controlling
Centrifugally operated, so as to ensure cell block and effective parcel of matrix, effectively prevents cell loss, and then improve cell block embedding matter
Amount；Moreover, removably being combined by using embedding base and centrifuge tube, the collection of cell block can be once realized, enters one
Step improves cell block embedding efficiency.
Corresponding with a kind of cell block embedding machine embodiment that the utility model is provided, the utility model additionally provides one kind
Cell block embedding method based on above-mentioned cell block embedding machine, this method comprises the following steps：
Step S101：Determine that cell block embeds parameter by the dyeing and SABC of analyzing paraffin section, wherein, it is described
Embedding parameter includes cell block embedding matrix type, substrate concentration, matrix parcel temperature, centrifuge speed and centrifuging temperature；
When it is implemented, the agar of various concentrations is prepared as cellular matrix, and by the agar of various concentrations respectively in different temperature
It is lower to add in above-mentioned cell block, and centrifuged respectively in high-temperature gradient centrifuge, compare centrifugation before and after addition Agar substrate
Cell block size, inquire into the conditions such as suitable centrifuging temperature, it is ensured that cell block can be wrapped up by effective aggregation in Agar substrate
And keep good form；The cell block for having wrapped up Agar substrate is separated, into follow-up gradient alcohol dehydration and paraffin bag
Bury program and paraffin section is carried out to cell block, on the one hand carry out normal dyeing, observe cellular morphology, on the other hand carry out cell
Groupization, observes groups of cells result, gropes optimal Agar substrate formula and high-temperature gradient according to cellular morphology and groupization result
Centrifugal condition；
Step S102：Centrifuged by regular growth, remove supernatant；
Step S103：Assembling embedding base and centrifuge tube, adds matrix in centrifuge tube, and will embedding base and from
The embedding component of heart test tube composition is installed on centrifuge；
Step S104：Under the conditions of the centrifuge speed and centrifuging temperature during the cell block embeds parameter, centrifuge pair
Cell block and the mixed liquor of matrix carry out high-temperature gradient centrifugation；In the specific implementation, the control of centrifuging temperature condition is at 20 DEG C to 70
In the range of DEG C；
Step S105：Centrifuge tube is taken apart with embedding base, embedding base closes the lid, carry out follow-up (fixation) dehydration
Embedding.
In order to realize the accurate temperature controlling of different temperatures gradient, in the utility model embodiment, alternatively, the centrifuge
High-temperature gradient centrifugation is carried out to cell block and the mixed liquor of matrix, including：
Step S1041：The heating-up temperature of primary heater is set, under the first heating-up temperature, to cell block and matrix
Mixed liquor is centrifuged；It is achieved in that the mixed liquor of cell block and matrix is centrifuged under the first heating-up temperature；
Step S1042：Set the heating-up temperature of each secondary heater group, under the second heating-up temperature, to cell block and
The mixed liquor of matrix is centrifuged., can be with when needing to carry out second and centrifuging or when needing to be adjusted centrifuging temperature
Heated using secondary heater group, centrifuging temperature is adjusted, so as to when reaching the second heating-up temperature, be mixed to cell block and matrix
Close liquid and carry out second of centrifugation.
Certainly, it is necessary to which explanation, in specific centrifugal process, the process of above-mentioned high-temperature gradient centrifugation is only an example
Property embodiment, in the specific implementation, can carry out it is any repeatedly, the centrifugally operated of different temperatures gradient；Moreover, it is also possible to one
During secondary centrifugation, by adjusting the power of different secondary heater groups, the centrifugation of various kinds of cell block multi-temperature gradient is realized
Operation.
As seen from the above-described embodiment, a kind of cell block embedding method that the utility model embodiment is provided, by analyzing stone
The dyeing of wax section and SABC determine that cell block embeds parameter, wherein, the embedding parameter includes cell block and embeds matrix
Type, substrate concentration, matrix parcel temperature, centrifuge speed and centrifuging temperature；Centrifuged by regular growth, remove supernatant；
Matrix is added in centrifuge tube；Under the conditions of the centrifuge speed and centrifuging temperature during the cell block embeds parameter, centrifugation
Machine carries out high-temperature gradient centrifugation to cell block and the mixed liquor of matrix；By the cell block wrapped by matrix the separation after centrifugation,
Dehydration embedding.In cell block embedding process, multiple thermogrades can be carried out to cell block by centrifuge accurate temperature controlling
Centrifugally operated, so as to ensure cell block and effective parcel of matrix, effectively prevents cell loss, and then improve cell block embedding matter
Amount；Moreover, removably being combined by using embedding base and centrifuge tube, the collection of cell block can be once realized, enters one
Step improves cell block embedding efficiency.
It should be noted that herein, the relational terms of such as " first " and " second " or the like are used merely to one
Individual entity or operation make a distinction with another entity or operation, and not necessarily require or imply these entities or operate it
Between there is any this actual relation or order.Moreover, term " comprising ", "comprising" or its any other variant are intended to
Cover including for nonexcludability, so that process, method, article or equipment including a series of key elements not only include those
Key element, but also other key elements including being not expressly set out, or also include for this process, method, article or set
Standby intrinsic key element.In the absence of more restrictions, the key element limited by sentence "including a ...", it is not excluded that
Also there is other identical element in the process including the key element, method, article or equipment.
Described above is only embodiment of the present utility model, is made skilled artisans appreciate that or realizing this
Utility model.A variety of modifications to these embodiments will be apparent to one skilled in the art, institute herein
The General Principle of definition can in other embodiments be realized in the case where not departing from spirit or scope of the present utility model.
Therefore, the utility model is not intended to be limited to the embodiments shown herein, and is to fit to and original disclosed herein
The reason most wide scope consistent with features of novelty.
Claims (8)
1. a kind of cell block embedding machine, it is characterised in that including centrifuge and embedding component, wherein：
The centrifuge includes centrifuge pot, primary heater and centrifugation motor；The inner surface of the centrifuge pot is surrounded
Into centrifugal chamber；Primary heater is set on the bottom surface inwall of the centrifuge pot, along the side inner walls of the centrifuge pot；
The centrifugation motor is arranged on the bottom of centrifuge and the electric machine main shaft of centrifugation motor is stretched through the bottom surface of the centrifuge pot
Enter in the centrifugal chamber；Mounting seat is fixedly installed at the top of the centrifugation electric machine main shaft, centrifugal turntable is fixedly mounted on described
In mounting seat；
The centrifugal turntable includes the equally distributed turntable base of multiple circumferences；It is provided with the side wall of each turntable base non-
There is inclination angle in the axis of the fixing groove of insertion, the fixing groove and centrifugal turntable, and the fixing groove notch close to motor
Main shaft；
The embedding component includes embedding base and centrifuge tube；Be provided with first connecting portion on the embedding base, it is described from
The openend of heart test tube is detachably connected with the connecting portion；The articulated end of the centrifuge tube is provided with second connecting portion, institute
State second connecting portion with the fixing groove to match, the embedding component is slidably hung on adjacent discs by second connecting portion
In 2 fixing grooves of the opposite flank of bearing.
2. cell block embedding machine according to claim 1, it is characterised in that the embedding base includes box body, the box
Set in vivo fluted；It is provided with the side lateral wall of the box body at the top of wedge-shaped platform, the wedge-shaped platform and is provided with first
Neck；Orthogonal reinforcement is provided with the bottom surface of the groove, the reinforcement intersects at the center of groove floor；
Second neck is set on groove bottom wall on the position relative with first neck, and second neck is located at reinforcement muscle line
On；The connecting portion is round table-like structure, and the connecting portion is internally provided with the uniform cavity through round table-like structure, described
The bottom center of connecting portion and the center superposition of groove；The internal diameter of the connecting portion is less than or equal to the outer of centrifuge tube openend
Footpath.
3. cell block embedding machine according to claim 1 or 2, it is characterised in that the connecting portion and the centrifuge tube
Openend screw thread matching connection.
4. cell block embedding machine according to claim 3, it is characterised in that the embedding component also includes and the embedding
Set on the embedding lid that base matches, a side of the embedding lid match with the first neck the first buckle,
The second buckle matched with the second neck is set on another side.
5. cell block embedding machine according to claim 1, it is characterised in that at the bottom land position of the fixing groove, edge
The direction that stretches out of hold-down support is horizontally disposed with secondary heater；Two relative secondary heater groups of adjacent turntable base
Into a secondary heater group；Each separate temperature control of secondary heater group.
6. cell block embedding machine according to claim 1 or 5, it is characterised in that on each side wall of the turntable base
The spacing being provided with multiple fixing grooves being parallel to each other, the bottom land horizontal direction of adjacent fixing groove is more than or equal to centrifuge tube
Length, and the length of the fixing groove gradually increases along away from centrifugation electric machine main shaft direction.
7. cell block embedding machine according to claim 1, it is characterised in that the second connecting portion includes head rod
With the second connection；The head rod overlaps and vertical with the body of centrifuge tube with the axis of the second connecting rod；
The head rod can be respectively sleeved in corresponding fixing groove with the end of the second connecting rod.
8. cell block embedding machine according to claim 7, it is characterised in that the head rod and the second connecting rod
Length is unequal.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720076785.XU CN206408215U (en) | 2017-01-20 | 2017-01-20 | A kind of cell block embedding machine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720076785.XU CN206408215U (en) | 2017-01-20 | 2017-01-20 | A kind of cell block embedding machine |
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| CN206408215U true CN206408215U (en) | 2017-08-15 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106680065A (en) * | 2017-01-20 | 2017-05-17 | 济南英盛生物技术有限公司 | Cell block embedding machine and embedding method |
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- 2017-01-20 CN CN201720076785.XU patent/CN206408215U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106680065A (en) * | 2017-01-20 | 2017-05-17 | 济南英盛生物技术有限公司 | Cell block embedding machine and embedding method |
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| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Workshop 405, building 12, industrialization base of small and medium sized enterprises, No. 1777, Dazheng Road, high tech Zone, Jinan, Shandong Patentee after: SHANDONG YING SHENG BIOTECHNOLOGY Co.,Ltd. Address before: 250100, 1 floor, building 2766, Ying Lu, Ji'nan hi tech Zone, Shandong, China Patentee before: JINAN YING SHENG BIOTECHNOLOGY Ltd. |
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Feng Zhen Inventor after: Jing Yesong Inventor after: Mo Zhao Yuan Inventor before: Shi Guizhi Inventor before: Feng Zhen Inventor before: Jing Yesong Inventor before: Mo Zhao Yuan |