CN210900804U

CN210900804U - Tissue culture reactor for blueberries

Info

Publication number: CN210900804U
Application number: CN201921959472.4U
Authority: CN
Inventors: 胡重九; 陈相涛; 陈芳芳; 王方
Original assignee: Hangzhou Mumu Biotechnology Co Ltd
Current assignee: Hangzhou Mumu Biotechnology Co Ltd
Priority date: 2019-11-13
Filing date: 2019-11-13
Publication date: 2020-07-03
Anticipated expiration: 2029-11-13

Abstract

The utility model discloses a tissue culture reactor for blueberry, the on-line screen storage device comprises a base, the middle part on base top is inserted and is established reaction vessel, and reaction vessel's top has cup jointed the closing cap, the middle part of closing cap is inserted and is equipped with the intake pipe, and the bottom of intake pipe extends to the inside bottom of reaction vessel, the gas outlet has evenly been seted up on the surface of intake pipe, and the gas outlet all runs through the intake pipe inner wall, the left and right sides at closing cap middle part has all run through the outlet duct, and one side that the intake pipe was kept away from to the outlet duct all is provided with the exhaust head, the top of exhaust head all is linked together with the outlet duct, the inside of exhaust head all is provided with the chock, and the top of. The utility model discloses increase the range of reaction vessel vibration to improve the fluctuation range of the inside blueberry tissue base fluid of reaction vessel, increase the mixed efficiency of biography oxygen of blueberry tissue base fluid, and guaranteed that reaction vessel is inside to be in sterile condition, it is effectual to cultivate.

Description

Tissue culture reactor for blueberries

Technical Field

The utility model relates to a cultivate reactor technical field, specifically be a tissue culture reactor for blueberry.

Background

The plant tissue culture is called in-vitro culture, which means that tissues, organs or cells and the like meeting the requirements are separated from plant bodies, the culture is carried out under the condition of manual control through aseptic operation to obtain regenerated complete plants or produce other products with economic value, the tissue culture technology is widely applied to the culture of blueberries, the conventional tissue culture reactor is generally used for mixing and oxygen transferring in a stirring mode, the histiocytes in the culture medium liquid are easy to damage, while the conventional bubble culture reactor has small damage to the histiocytes, but the fluctuation in the culture medium liquid is small, and the mixed oxygen transfer efficiency is low.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a tissue culture reactor for blueberry to the inside undulant less of culture medium liquid mixes the problem of oxygen transfer inefficiency in the current bubble type culture reactor who proposes among the solution above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a tissue culture reactor for blueberry, includes the base, the middle part on base top is inserted and is equipped with reaction vessel, and reaction vessel's top has cup jointed the closing cap, the middle part of closing cap is inserted and is equipped with the intake pipe, and the bottom of intake pipe extends to the inside bottom of reaction vessel, the gas outlet has evenly been seted up on the surface of intake pipe, and the gas outlet all runs through the intake pipe inner wall, the left and right sides at closing cap middle part has all run through the outlet duct, and the outlet duct one side of keeping away from the intake pipe all is provided with the exhaust head, the top of exhaust head all is linked together with the outlet duct, the inside of exhaust head all is provided with the chock, and the top of chock all welds there is the second spring, the top of second spring all extends to the inside and.

Preferably, the inner wall of the top end of the base is uniformly welded with first springs, the other ends of the first springs are all welded with supporting sheets, and the supporting sheets are attached to the reaction vessel.

Preferably, the outer side of the top end of the reaction container is provided with an external thread, and the inner wall of the sealing cover is provided with an internal thread.

Preferably, the sealing ring is embedded in the inner wall of the top end of the sealing cover, and the sealing ring is attached to the edge of the top end of the reaction container.

Preferably, the outlet duct all is L shape, the orientation of exhaust head opening part all is downwards, the middle part of exhaust head bottom all is provided with the sponge net.

Preferably, the top ends of the exhaust head and the chock block are conical, and the cross section area of the bottom end of the exhaust head is larger than that of the bottom end of the chock block.

Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses a be provided with intake pipe and first spring, aseptic oxygen enters into blueberry tissue base liquid through the gas outlet in the intake pipe inside, make the inside gassing of blueberry tissue base liquid, thereby pass oxygen to the histocyte mixture in the blueberry tissue base liquid, and blueberry tissue base liquid receives the promotion of oxygen and can produce certain undulant, make reaction vessel produce slight vibration, resilience force through first spring promotes reaction vessel rebound that resets, increase the range of reaction vessel vibration, thereby improve the fluctuation range of the inside blueberry tissue base liquid of reaction vessel, increase the mixed efficiency of passing oxygen of blueberry tissue base liquid.

The utility model discloses a be provided with the outlet duct, aseptic oxygen is after blueberry tissue base liquid, in the middle of entering into the outlet duct, promote the chock move down for gas passes exhaust head discharge device, when reaction vessel is inside not have the air escape, resilience force pulling chock through the second spring is blockked up the top of exhaust head, thereby inside effectually having prevented that outside air from entering into reaction vessel through exhaust head and outlet duct, has guaranteed that reaction vessel is inside to be in sterile condition, cultivates effectually.

Drawings

Fig. 1 is a schematic front sectional view of the present invention;

fig. 2 is an enlarged schematic view of a point a in fig. 1 according to the present invention;

fig. 3 is an enlarged schematic view of the position B in fig. 1 according to the present invention;

fig. 4 is a front view schematically illustrating the present invention.

In the figure: 1. a base; 2. a reaction vessel; 3. a first spring; 4. a support sheet; 5. sealing the cover; 6. a seal ring; 7. an air inlet pipe; 8. an air outlet; 9. an air outlet pipe; 10. an exhaust head; 11. a sponge web; 12. a chock block; 13. a second spring.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, the present invention provides an embodiment:

a tissue culture reactor for blueberries comprises a base 1, a reaction container 2 is inserted in the middle of the top end of the base 1, first springs 3 are uniformly welded on the inner wall of the top end of the base 1, supporting pieces 4 are welded at the other ends of the first springs 3, the supporting pieces 4 are attached to the reaction container 2, when bubbles enter the blueberry tissue base liquid, the blueberry tissue base liquid is pushed by oxygen to generate certain fluctuation, so that the reaction container 2 generates fine vibration, the reaction container 2 vibrates and inclines to press down the supporting pieces 4, the first springs 3 are compressed, the reaction container 2 is pushed to reset and rebound by resilience force of the first springs 3, the vibration amplitude of the reaction container 2 is increased, the fluctuation amplitude of the blueberry tissue base liquid inside the reaction container 2 is increased, the oxygen transfer efficiency of blueberry tissue base liquid mixing is increased, a sealing cover 5 is sleeved on the top end of the reaction container 2, the outer side of the top end of the reaction container 2 is provided with an external thread, the inner wall of the sealing cover 5 is provided with an internal thread, when workers need to clean the interior of the reaction container 2, the sealing cover 5 can be rotated, the sealing cover 5 is detached from the top end of the reaction container 2 through a thread structure, the workers can clean the reaction container 2 conveniently, a sealing ring 6 is embedded in the inner wall of the top end of the sealing cover 5, the sealing ring 6 is attached to the edge of the top end of the reaction container 2, the sealing performance between the reaction container 2 and the sealing cover 5 is effectively improved, bacteria are prevented from entering the interior of the reaction container 2 along with external air, an air inlet pipe 7 is inserted in the middle of the sealing cover 5, the bottom end of the air inlet pipe 7 extends to the bottom end of the interior of the reaction container 2, air outlets 8 are uniformly formed;

the left side and the right side of the middle part of the sealing cover 5 are both penetrated by air outlet pipes 9, one sides of the air outlet pipes 9 far away from the air inlet pipe 7 are both provided with air outlet heads 10, the top ends of the air outlet heads 10 are both communicated with the air outlet pipes 9, the air outlet pipes 9 are all L-shaped, the directions of openings of the air outlet heads 10 are all downward, the middle parts of the bottom ends of the air outlet heads 10 are both provided with sponge nets 11, dust and bacteria are prevented from falling into the air outlet heads 10 and entering the reaction container 2 through the air outlet pipes 9, the interiors of the air outlet heads 10 are both provided with plugs 12, the top ends of the plugs 12 are both welded with second springs 13, the top ends of the second springs 13 extend into the interiors of the air outlet pipes 9 and are welded and fixed with the inner walls of the air outlet pipes 9, the top ends of the air outlet heads 10 and the plugs 12 are both conical, the cross section area of the bottom ends, promote the chock 12 and remove downwards, stretch second spring 13 to pass the exhaust head 10 and discharge the device, when reaction vessel 2 is inside not to have the air to discharge, the resilience force through second spring 13 stimulates chock 12 and blocks up the top of exhaust head 10, thereby effectually prevented that outside air from entering into reaction vessel 2 inside through exhaust head 10 and outlet duct 9, guaranteed that reaction vessel 2 is inside to be in sterile condition, cultivates effectually.

The working principle is as follows: firstly, a worker connects the top end of an air inlet pipe 7 with an external air source, sterile oxygen enters the air inlet pipe 7 through the top end of the air inlet pipe 7 and enters the blueberry tissue base liquid through an air outlet 8, bubbles are generated inside the blueberry tissue base liquid, so that mixed oxygen transfer is performed on tissue cells in the blueberry tissue base liquid, when the bubbles enter the blueberry tissue base liquid, the blueberry tissue base liquid is pushed by the oxygen to generate certain fluctuation, so that the reaction container 2 generates fine vibration, the reaction container 2 is vibrated and inclined to press down some supporting sheets 4, so that the first spring 3 is compressed, the reaction container 2 is pushed to reset and rebound through the resilience force of the first spring 3, the vibration amplitude of the reaction container 2 is increased, the fluctuation amplitude of the blueberry tissue base liquid inside the reaction container 2 is improved, the mixed oxygen transfer efficiency of the blueberry tissue base liquid is increased, after the sterile oxygen passes through the blueberry tissue base liquid, enter into in the middle of the outlet duct 9, promote chock 12 and remove downwards, stretch second spring 13 to pass the exhaust head 10 and discharge the device, when reaction vessel 2 is inside not to have the air to discharge, the resilience force through second spring 13 stimulates chock 12 and blocks up the top of exhaust head 10, thereby effectually prevented that outside air from entering into reaction vessel 2 through exhaust head 10 and outlet duct 9 inside, guaranteed that reaction vessel 2 is inside to be in sterile condition.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. A tissue culture reactor for blueberries, comprising a base (1), characterized in that: a reaction container (2) is inserted in the middle of the top end of the base (1), a sealing cover (5) is sleeved on the top end of the reaction container (2), an air inlet pipe (7) is inserted in the middle of the sealing cover (5), the bottom end of the air inlet pipe (7) extends to the bottom end inside the reaction vessel (2), the surface of the air inlet pipe (7) is uniformly provided with air outlets (8), the air outlets (8) all penetrate through the inner wall of the air inlet pipe (7), the air outlet pipes (9) all penetrate through the left side and the right side of the middle part of the sealing cover (5), and one side of the air outlet pipe (9) far away from the air inlet pipe (7) is provided with an air outlet head (10), the top end of the exhaust head (10) is communicated with the air outlet pipe (9), the inside of the exhaust head (10) is provided with a chock block (12), and the top of chock (12) all welds second spring (13), the top of second spring (13) all extends to the inside and air outlet pipe (9) inner wall welded fastening of air outlet pipe (9).

2. The tissue culture reactor for blueberries according to claim 1, wherein: evenly weld on the inner wall on base (1) top has first spring (3), and the other end of first spring (3) all welds and has backing sheet (4) to backing sheet (4) all laminate mutually with reaction vessel (2).

3. The tissue culture reactor for blueberries according to claim 1, wherein: the outer side of the top end of the reaction container (2) is provided with an external thread, and the inner wall of the sealing cover (5) is provided with an internal thread.

4. The tissue culture reactor for blueberries according to claim 1, wherein: sealing washer (6) are embedded to have on the inner wall on closing cap (5) top, and sealing washer (6) laminate mutually with the edge on reaction vessel (2) top.

5. The tissue culture reactor for blueberries according to claim 1, wherein: the air outlet pipes (9) are L-shaped, the opening of the exhaust head (10) faces downwards, and the middle of the bottom end of the exhaust head (10) is provided with a sponge net (11).

6. The tissue culture reactor for blueberries according to claim 1, wherein: the top of the exhaust head (10) and the top of the chock block (12) are both conical, and the cross section area of the bottom end of the exhaust head (10) is larger than that of the bottom cross section of the chock block (12).