CN210893868U

CN210893868U - Simple and easy making devices of one shot forming tissue chip acceptor wax stone

Info

Publication number: CN210893868U
Application number: CN201921639330.XU
Authority: CN
Inventors: 周军; 段玲; 谢余澄; 吕孟兴; 高燕; 阿智祥; 李霁伟
Original assignee: Kunming Childrens Hospital
Current assignee: Kunming Childrens Hospital
Priority date: 2019-09-29
Filing date: 2019-09-29
Publication date: 2020-06-30
Anticipated expiration: 2029-09-29

Abstract

The utility model discloses a simple and easy making devices of one shot forming tissue chip receptor wax stone, including the embedding box, the die block, top cap and contact pin device, but the embedding box is installed on the die block with removable fraction, the detachable top cap of installing in embedding box top, all offer on the capping of shown top cap and the bottom surface of embedding box etc. big, a plurality of circular port of equivalent, and the centre of a circle of a set of circular port corresponding on the upper and lower two-layer face all is in the one-to-one alignment distribution on the same vertical line, the contact pin device is including arranging the number of rows and quantity and the corresponding unanimity of circular port quantity on the top cap capping, and a plurality of contact pins of the downward vertical range distribution that length equals, the diameter of contact. The utility model discloses the tissue chip receptor wax stone of preparation is one shot forming, need not manual single punching, has overcome current ordinary preparation appearance and need punch through a lot of and could prepare the technical obstacle of array wax stone to the shortcoming of punching in-process wax stone fracture has been stopped completely.

Description

Simple and easy making devices of one shot forming tissue chip acceptor wax stone

Technical Field

The utility model relates to an application of a simple manufacturing device of a one-step formed tissue chip receptor wax block in the field of clinical pathology and tissue chip basic research.

Background

Tissue chip (also called tissue microarray, TAM) is an important biochip technology which appears after gene chips and protein chips, and is one of the important methods for genomics and proteomics research in the post-genome era. The tissue chip (tissue chip) technology is a tissue array chip formed by orderly integrating dozens of thousands of tiny tissues on a glass slide, is a high-flux biochip technology rapidly developed in recent years, and can overcome the limitation that the conventional pathological tissue section technology cannot rapidly and efficiently detect a plurality of samples. The method has the greatest potential effects that the research on gene and protein levels is combined with tissue morphology, the same experiment index is applied, the idea of rapidly researching a large number of different tissue samples (high flux and multiple samples) is realized, the experiment error is reduced, the efficiency of the tissue morphology research is improved by dozens of times or hundreds of times, the experiment materials and reagents are saved, the experiment result has more reliable comparability, and the method has wide attention of global scientists due to the huge application potential and practical value.

The preparation of the tissue chip combines the semi-automatic operation of a tissue chip preparation instrument with a manual simple manufacturing method. At present, the related materials such as a commercialized tissue chip preparation instrument, an acceptor wax block and the like are high in price and are protected by patents, so that the tissue chip preparation instrument is not suitable for popularization. For the primary medical institution and clinical pathology researchers, a simple and easy-to-implement manual manufacturing method is particularly important. The tissue chip receptor wax block is difficult to manufacture manually, the current common method is a hollow needle punching method, a mold casting method and the like, the manufactured receptor wax block is an independent wax block, and the receptor wax block needs to be combined with an embedding box molten wax again in the later period. In addition, because the proportion of the hardness and the flexibility of the common embedding wax is not ideal, the hollow needle punching method is easy to cause wax block fracture, uneven punching size, uneven arrangement or 'break', the mold casting method is easy to cause failure in demolding, the two methods for manufacturing the receptor wax block have more steps, and the two methods are difficult to be used for manufacturing the high-flux receptor wax block and cannot be used for batch manufacturing.

Disclosure of Invention

The invention aims to provide a simple device for manufacturing a receptor wax block of a one-step formed tissue chip, which can be used for quickly and manually manufacturing the receptor wax block which is integrally formed, does not need to punch at the later stage and fuse the wax block and an embedding box, and can directly implant a donor tissue core. The method really achieves the purpose of obtaining the integrally formed tissue chip receptor wax block by a simple and rapid manual manufacturing method, and is more suitable for basic medical institutions.

Specifically, the utility model discloses a realize like this:

simple and easy making devices of one shot forming tissue chip receptor wax stone, including the embedding box, the die block, top cap and contact pin device, the embedding box is installed on the die block with removable fraction, the detachable top cap of installing in embedding box top, all seted up on the capping of shown top cap and the bottom surface of embedding box and wait big, a plurality of circular port of equivalent, and the centre of a circle of a set of circular port corresponding on the upper and lower two-layer face all is in same vertical line one-to-one alignment distribution, the contact pin device is including arranging the number and quantity and the corresponding unanimity of circular port quantity on the top cap capping, and a plurality of contact pins of downward vertical range distribution that length equals, the diameter of contact pin is not more than the.

Furthermore, the pin inserting device comprises a flat plate, the pins are vertically installed on the bottom surface of the flat plate downwards respectively, and a handle is arranged on the top surface of the flat plate.

Further, the embedding box material makes for rigid plastic or stainless steel, and the size of a dimension of embedding box suits with the size of the common slicer chuck in market, the die block is made for the stainless steel metalwork, the top cap is the same with the embedding box material, can detain firmly on the embedding box, the contact pin is stainless steel material cylinder physique.

Furthermore, the length of the contact pin is 5cm, and the aperture size of the circular hole is 1 mm-2 mm.

Furthermore, the cover surface of the top cover and the bottom surface of the embedding box are 30 hole types, the number of array through holes is 10 × 3, the hole diameter is 3mm, and the hole distance is 3mm, or 60 hole types, the number of array through holes is 10 × 6, the hole diameter is 2mm, and the hole distance is 2mm, or 90 hole types, the number of array through holes is 10 × 9, the hole diameter is 1.5mm, and the hole distance is 1mm, or 150 hole types, the number of array through holes is 12 × 15, the hole diameter is 1mm, and the hole distance is 1mm, and the number of the contact pins is equal to the number of the contact pins.

Introduction of the working principle: when the device is used, the bottom die and the embedding box are installed in a butt joint mode, receptor wax block wax liquid is poured into the embedding box and permeates into the bottom die until the embedding box is filled with the receptor wax block wax liquid, then the top cover is covered, the contact pins penetrate through the circular holes of the top cover from top to bottom and then reach the bottom die until the contact pins also penetrate through the corresponding circular holes in the bottom of the embedding box, all the circular holes are fully inserted with the contact pins, the volume of the contact pins is squeezed to occupy partial space of the receptor wax block wax liquid, and after the receptor wax block wax liquid is cooled to form receptor wax blocks, the contact pins are completely pulled out, so that the drilling operation of the receptor wax blocks is completed, and the tissue cores are convenient to fill in a later; the design that a plurality of contact pins are arranged on the flat plate can keep simultaneously and synchronously carrying out contact pin and needle pulling operation on all round holes, and is efficient and convenient; the flat board lacks the square block body of the design of a horn piece, is used for when the contact pin, and the space of the partial acceptor wax stone wax liquid that the needle body occupy is outwards discharged from remaining circular hole, inserts the square block body of last needle again, then can avoid the wax liquid of the volume of occupying to extrude or spill over from circular hole surface top from the die block gap in a large number, leads to being difficult to pull out the problem of needle.

The utility model discloses a beneficial effect introduces:

the simple device for manufacturing the tissue chip receptor wax block by one-step molding is characterized in that the bottom of the embedding box and the circular hole on the top cover are in one-to-one correspondence, the hole diameters of the bottom small hole and the circular hole of the top cover are respectively set to be 1mm, 1.5mm and 2mm, the tissue chip receptor wax block manufactured by the simple device is formed by one-step molding, manual punching is not needed, the technical obstacle that the array wax block can be manufactured by multiple times of punching in the existing common preparation instrument is overcome, and the defect that the wax block is cracked in the punching process is completely avoided; the number of the micro-arrays is increased, and the integral forming can ensure that the spacing between the holes is reduced to the minimum, so that the number of the micro-arrays in the same area is increased, and more tissue cores can be implanted into the same wax block; the difference between the receptor wax block batches is reduced. The mold can be compatible with plastic embedding boxes commonly adopted in the market at present, facilitates the unified arrangement of data, and is a very good tissue chip receptor wax block manufacturing device.

Drawings

FIG. 1 is a schematic structural view of an embedding cassette;

FIG. 2 is a schematic view showing the structural installation of a simple device for manufacturing a receptor wax block of a one-step molded tissue chip;

FIG. 3 is a schematic view of a plate with pins;

FIG. 4 is a schematic structural view of a stabilizing frame;

FIG. 5 is a schematic structural view of the present invention using a stable operation frame;

FIG. 6 is a schematic structural view of the present invention after the insertion of the contact pin;

FIG. 7 is a cross-sectional view of the structure of the present invention after insertion of the contact pin;

FIG. 8 is a schematic structural view of a cube;

FIG. 9 is a schematic view of a gear assembly having a geared plate;

FIG. 10 is a schematic structural view of a simple apparatus for manufacturing a receptor wax block of a one-step-formed tissue chip in example 5.

Wherein: 1 embedding box, 2 bottom dies, 3 top covers, 4 circular holes, 5 pins, 6 flat plates, 61 handles, 62 square blocks, 63 stable operation frames, 64 placing grooves, 65 hasp fixing strips, 66 guide posts, 67 guide holes, 68 external gear nuts, 69 transmission gears, 70 main gears, 71 lifting thumb wheels, 72 screw posts and 8 flat plate covers

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in detail with reference to the accompanying drawings. It should be understood that the description is intended to be illustrative only and is not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.

Embodiment 1 as shown in fig. 1 and 2, a simple device for manufacturing a wax block of a tissue chip receptor for one-step molding comprises an embedding box 1, a bottom die 2, a top cover 3 and a pin inserting device, wherein the embedding box 1 is detachably mounted on the bottom die 2, the top cover 3 is detachably mounted at the top of the embedding box 1, circular holes 4 which are equal in size and quantity and are distributed according to 10 × 3, the aperture is 3mm, and the hole interval is 3mm are respectively formed in the cover surface of the top cover 3 and the bottom surface of the embedding box 1, the centers of a group of corresponding circular holes 4 on the upper layer and the lower layer of the cover are respectively aligned and distributed on the same vertical line, the pin inserting device comprises 30 downward vertically arranged and distributed pins 5 which are arranged and have the same quantity and length as the circular holes 4 on the cover surface of the top cover 3, the diameter of the pins 5 is not greater than that of the circular holes 4, when the device is used, the top cover 3 is opened, the wax block is filled with the embedding box 1, the wax liquid of the wax block is poured into the bottom die 2 and the embedding box 1, the wax block is poured into the embedding box, the wax block is slowly pulled out, and solidified, and the tissue chip wax block is obtained, and the tissue chip wax block is held by the device, and the tissue chip wax block.

Example 2: on the basis of embodiment 1, as shown in fig. 3, all the contact pins 5 are collectively installed on the bottom surface of a flat plate 6, the contact pins 5 are respectively vertically installed downwards on the bottom surface of the flat plate 6, a handle 61 is arranged on the top surface of the flat plate 6, when the device is used, the handle 61 is held by hand, and after the device is used for hole positions of the circular holes 4 on the top cover 3, all the contact pins 5 are slowly and downwards inserted into the circular holes 4, so that the whole process is carried out, and the inserting and extracting efficiency can be effectively improved.

In the above two embodiments, the embedding box 1 is made of rigid plastic or stainless steel, the size of the embedding box 1 is matched with the size of a slicing machine chuck commonly used in the market, the bottom die 2 is made of a stainless steel metal piece, the top cover 3 is the same as the embedding box 1 in material and can be firmly buckled on the embedding box 1, and the contact pin 5 is a stainless steel cylindrical body.

The length of the contact pin 5 is 5cm, and the aperture size of the circular hole 4 is 1 mm-2 mm.

Embodiment 3, as shown in fig. 8, based on embodiment 2, the cover surface of the top cover 3 and the bottom surface of the embedding box 1 are 60-hole type, the number of through holes in the array is 10 × 6, the hole diameter is 2mm, and the hole distance is 2mm, the flat plate 6 is a special-shaped flat plate 6, one end corner of which lacks a square block 62 with at least one insertion pin 5, the square block 62 is formed separately, and can form a complete flat plate 6 with the special-shaped flat plate 6, the flat plate 6 lacks a square block 62 with a corner block design, and is used for discharging part of the space occupied by the needle body for receiving the wax block from the residual circular holes 4 outwards when the insertion pin 5 is inserted, and then the problem that the needle is difficult to pull due to the fact that the wax liquid in the occupied volume of the circular holes is extruded from the gaps of the bottom mold 2 or a large amount overflows from the upper surface of the bottom mold 4 when the square block.

Embodiment 4 on the basis of

embodiment

2 or 3, as shown in fig. 4 to 7, the circular hole 4 is provided with 90 holes, the number of through holes in the array is 10 × 9, the hole diameter is 1.5mm, the hole distance is 1mm, and the pin inserting device further includes a stabilizing operation frame 63, a placement groove 64 is provided in the middle of the stabilizing operation frame 63, two hinged snap fastener fixing strips 65 are provided on two side edges of the placement groove 64, the bottom mold 2 and the embedding box 1 are placed in the placement groove 64 of the stabilizing operation frame 63, the two hinged snap fastener fixing strips 65 are located above two side edges of the top of the embedding box 1 and do not block the circular hole 4, a guide post 66 extending vertically upward is provided on an end face of at least one snap fastener fixing strip 65, a guide hole 67 adapted to the guide post 66 is provided on at least one end corner of the flat plate 6, when the pin inserting device is used in this embodiment, the two snap fastener fixing strips 65 are turned upward, the bottom mold 2 and the embedding box 1 are placed in the placement groove 64 of the stabilizing operation frame 63, the bottom mold 2 and the embedding box 1 are aligned with the wax liquid receptor block, the wax liquid is poured over the two snap fastener fixing strips 65, the embedding box 6, the needle inserting device is aligned with the guide post 6, the needle inserting handle 6 and the needle inserting head 6, the needle inserting device can be slowly, the needle inserting device can be more efficiently, the needle inserting device can be performed in the needle inserting device, the needle.

Embodiment 5 as shown in fig. 9 and 10, the structure is further optimized on the basis of embodiment 4, the circular hole 4 array is 150 holes, the array through holes 12 × 15, the hole diameter 1mm, and the hole pitch 1mm, the number of pins 5 on the plate 6 is equal to that of the circular hole array, the guide posts 66 are two screw posts 72 with screw threads respectively located at the tail end and the tip end of two snap-fastening strips 65, the guide holes 67 are opened at two opposite end corners of the plate 6, and screw caps 68 adapted to the screw posts 72 are installed above the guide holes 67, the external screw caps 68 are screwed on the screw posts 72, the two external screw caps 68 are respectively in transmission connection with the external gear 70 through a transmission gear 69, the main gear 70 is installed under the handle 61 and fixedly connected with a lifting thumb wheel 71 sleeved on the handle 61, the lifting thumb wheel 71 can manually drive the external gear 70 to rotate on the handle 61, the steps of the wax-receiving body wax-liquid of the external gear is the same as that of the previous embodiment, the main gear 70 is installed under the handle 61 and fixedly connected with the lifting thumb wheel 71 sleeved on the handle 61, the lifting thumb wheel 71 is capable of being driven by the lifting and the external gear 70, the lifting and the external gear 70 of the lifting and the external gear 70, the lifting and the flat plate 6 are capable of being driven by the lifting and the flat plate 6, thereby capable of keeping the flat plate 6 rotating, the whole flat plate 5 rotating, the flat plate 6, the flat plate 5, the flat plate 6 rotating speed of being more stably maintained, the handle is capable of being more stably maintained, the same, the outer gear 70 being capable of being lifted and being more stably maintained, the lifting and being lifted and of being lifted and being lifted and lifted by the flat plate when the flat.

It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the invention and are not to be construed as limiting the invention. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.

Claims

1. Simple and easy making devices of one shot forming tissue chip receptor wax stone, a serial communication port, including the embedding box, the die block, top cap and contact pin device, the embedding box is installed on the die block with removable fraction, the detachable top cap of installing in embedding box top, all seted up on the capping of shown top cap and the bottom surface of embedding box etc. greatly, a plurality of circular port of equivalent, and the centre of a circle of a set of circular port corresponding on the upper and lower two-layer face all is in same vertical line one-to-one alignment distribution, the contact pin device is including arranging the corresponding unanimity of circular port quantity on number and the top cap capping, and a plurality of contact pins of the downward vertical range distribution that length equals, the diameter of contact pin is not more than the diameter.

2. The apparatus for easily manufacturing the receptor wax of one-shot tissue chip as claimed in claim 1, wherein the pin inserting device comprises a plate, the pins are respectively vertically installed downwards on the bottom surface of the plate, and the top surface of the plate is provided with a handle.

3. The simple device for manufacturing the receptor wax block of the one-step formed tissue chip according to claim 1 or 2, wherein the embedding box is made of rigid plastic or stainless steel, the size of the embedding box is matched with the size of a chuck of a common microtome in the market, the bottom die is made of a stainless steel metal piece, the top cover and the embedding box are made of the same material and can be firmly buckled on the embedding box, and the contact pin is a stainless steel cylindrical body.

4. The device for simply manufacturing the receptor wax block of the one-step formed tissue chip according to claim 3, wherein the length of the contact pin is 5cm, and the diameter of the circular hole is 1mm to 2 mm.

5. The device for easily manufacturing the receptor wax block of the one-step formed tissue chip as claimed in claim 3, wherein the cover surface of the top cover and the bottom surface of the embedding box have 30 holes, the number of the array through holes is 10 × 3, the aperture is 3mm, and the hole spacing is 3mm, or

60 pass with array through holes 10 × 6, aperture 2mm, hole spacing 2mm, or

90 pass, array through hole number 10 × 9, aperture 1.5mm, hole spacing 1mm, or

150 hole patterns, the number of array through holes is 12 × 15, the aperture is 1mm, and the hole spacing is 1 mm;

the number of the pins is equal to the number of the pins.

6. The device for easily manufacturing the receptor wax block of the one-step formed tissue chip according to claim 2, wherein the flat plate is a special-shaped flat plate with one end corner lacking a square block body with at least one contact pin, and the square block body is formed separately and can form a complete flat plate together with the special-shaped flat plate.

7. The simple and easy making device of one shot forming tissue chip receptor wax stone as claimed in claim 2 or 6, characterized in that, the simple and easy making device of one shot forming tissue chip receptor wax stone still includes and stabilizes the handling frame, stabilize the handling frame middle part and be the standing groove, both sides limit department is equipped with two articulated hasp fixed strips on the standing groove, die block and embedding box are placed in the standing groove of stabilizing the handling frame, two articulated hasp fixed strips are located the both sides limit top at embedding box top and do not shelter from the circular port, be provided with the guide post that upwards extends perpendicularly on the terminal surface of at least one hasp fixed strip, set up the guiding hole with guide post looks adaptation on dull and stereotyped at least one end angle.

8. The device for easily manufacturing the once-formed tissue chip receptor wax block according to claim 7, wherein the two guiding posts are respectively located at the tail end and the end of the two fastening strips, the guiding posts are threaded screw posts, the guiding holes are formed in two opposite end corners of the flat plate, external gear nuts matched with the screw posts are installed above the guiding holes, the external gear nuts are screwed on the screw posts, the external gears of the two external gear nuts are respectively in transmission connection with a main gear, the main gear is installed below the handle in a penetrating manner and is fixedly connected with a lifting shifting wheel sleeved on the handle, and the lifting shifting wheel can manually drive the main gear to rotate on the handle.

9. The apparatus for easily manufacturing the receptor wax for one-step molding tissue chip according to claim 8, wherein the flat plate cover is integrally mounted on the main gear, the transmission gear and the external gear nut, and the flat plate cover, the main gear, the transmission gear, the external gear nut and the flat plate cover are packaged and molded.