CN201885912U - Mold for manufacturing tissue chip with one-step molding - Google Patents
Mold for manufacturing tissue chip with one-step molding Download PDFInfo
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- CN201885912U CN201885912U CN2010205880378U CN201020588037U CN201885912U CN 201885912 U CN201885912 U CN 201885912U CN 2010205880378 U CN2010205880378 U CN 2010205880378U CN 201020588037 U CN201020588037 U CN 201020588037U CN 201885912 U CN201885912 U CN 201885912U
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Abstract
The utility model provides a mold for manufacturing a tissue chip with one-step molding, belonging to the technical field of manufacture of the tissue chips and solving the problems of inconvenience in use and high cost of the existing mold for manufacturing the tissue chips. The mold comprises an array hole copper plate, an array needle copper plate and two L-shaped copper gaskets, wherein the array hole copper plate is provided with rectangular array through holes; one side of the array needle copper plate is provided with array needles which are in one-to-one correspondence to the array through holes; the cross section and the height of each array needle are the same as the section and the depth of the array through hole; the array needles are inserted into the array through holes; the two L-shaped copper gaskets are spliced into a rectangle and are padded between the array hole copper plate and the array needle copper plate; and the array needles are positioned in the two L-shaped copper gaskets. For the mold, the use is convenient, the structure is simple and the cost is low.
Description
Technical field
The utility model belongs to the organization chip manufacture technology field, relates to a kind of mould of making the one-shot forming organization chip.
Background technology
Organization chip (tissue chip) claims that again (tissue microarray TMA), is fitly to be arranged on the microslide and the histotomy of making to thousands of cells tens of to micro-array tissue.It is another the important biochip that occurs after genetic chip, protein-chip, is mainly used in the situation that research is expressed in different cell or tissues with a kind of gene or protein molecule, advantage such as have high flux, large sample, save time fast.From the clear and definite notion of proposition organization chips such as Kononen in 1998 and since confirming its effect, the tissue array technology development rapidly.This technology has been applied to human genome research, medical diagnosis and fundamental research, has especially shown huge potential at oncogene screening, tumour antigen screening and the searching aspects such as label relevant with tumour generation, development and prognosis.
Organization chip has the following advantages: (1) organization chip is a kind of high flux, large sample technology, and an experimental implementation can be finished tens times even thousands of times operation of traditional means of experiment, significantly improves the efficient of Histopathology research, saves time fast; (2) consumptive material is few, and required expense is 1/10～1/100 of traditional pathology method; (3) it is big to contain the biological information amount, helps to carry out the retrospective study of large sample.(3) the organization chip experiment condition is relatively more consistent, helps the contrived experiment control group; (4) can reduce in the traditional means of experiment batch in and batch between error; (6) view data of organization chip can adopt the automated analysis method, is convenient to data acquisition, storage and retrieval; (7) one block organization's chip wax stones contain the information of tens kinds even thousands of kinds of tissue specimens, the preservation of the wax stone of being convenient to file.The organization chip applied range can be used for basic medical research and clinical research, also can be used for the original position analysis of molecular diagnosis, treatment target spot location, antibody or new medicament screen and gene or protein expression etc.Organization chip is widely used in oncology studies.
The application prospect of organization chip is boundless, is very much loaded down with trivial details but make organization chip, needs to make hollow wax matrix, and secondary embedding etc. also need to have the organization chip that vacuumizes function and make instrument etc.And manufacturing process technical requirement height, distortion or cracking appear when making hollow wax matrix easily; Occur organizing core to merge not good during the secondary embedding easily or dewaxing is excessive; Tissue surface's out-of-flatness; Tissue displacement during section, even problem such as flake.At present more advanced organization chip instrument price is up to hundreds of thousands Renminbi, even homemade several ten thousand Renminbi of also wanting, and all very heavy.These shortcomings all make the development of organization chip and application be subjected to very big restriction, more make tissue array technology to promote at basic hospital or R﹠D institution.
Summary of the invention
The utility model has proposed a kind of mould of making the one-shot forming organization chip at the problems referred to above that prior art exists, and this mould is easy to use, and is simple in structure, and cost is low.
The utility model is realized by following technical proposal: a kind of mould of making the one-shot forming organization chip, it is characterized in that, this mould comprises the array hole copper coin, array pin copper coin and two L shaped copper backings, the array hole copper coin is provided with the array through-hole of rectangle, one side of array pin copper coin is provided with and above-mentioned array through-hole array pin one to one, the xsect of array pin is identical with the cross section and the degree of depth of array through-hole with height, array cushion is connected in the array through-hole, above-mentioned two L shaped copper backings are combined into rectangle, and fill up between array hole copper coin and array pin copper coin, above-mentioned array pin is positioned at two L shaped copper backings.
Owing to be lined with L shaped copper backing between array hole copper coin and the array pin copper coin, therefore on array through-hole, can reserve and the identical hole of L shaped copper backing thickness, these holes are holds wax pan, organization chip is positioned over and holds in the wax pan, through in the manufacturing process, extract L shaped copper backing out, press the array hole copper coin, the array pin of array pin copper coin can be with the organization chip jack-up of holding in the wax pan.
In the mould of above-mentioned making one-shot forming organization chip, also be placed with on the described array hole copper coin and hold wax pan, this holds the copper cubic frame structure that wax pan is a upper and lower opening, and top is bucket-shaped wide mouthful, and the bottom is the slot structure of tubular.
In the mould of above-mentioned making one-shot forming organization chip, described top of holding wax pan is provided with step-like birdsmouth.This birdsmouth is corresponding with the plastic embedding frame that pathology department uses, just can support.
In the mould of above-mentioned making one-shot forming organization chip, described array hole copper coin and array pin copper coin are rectangle, and both width are identical, the length that the length of array pin copper coin slightly is longer than the array hole copper coin; The long limit of described L shaped copper backing is slightly longer than the width of array pin copper coin.
In the mould of above-mentioned making one-shot forming organization chip, described array pin copper coin size 50 * 30mm
2, thick 3mm, pin spacing are 1mm, the diameter of array pin is 2mm;
In the mould of above-mentioned making one-shot forming organization chip, described array hole copper coin size is 45 * 30mm
2, thick 4mm, the aperture 2mm of array through-hole, pitch of holes 1mm;
In the mould of above-mentioned making one-shot forming organization chip, in the mould of above-mentioned making one-shot forming organization chip, described L shaped copper backing is thick to be 1mm, and hem width is 3mm.
Compared with prior art, four kinds of copper moulds of the utility model, simple in structure, cost is low, and is easy to use, and easy left-hand seat is smooth by the organization chip tissue surface of this Mold Making, integraty good, do not fall advantages such as core is unshift during section.
Description of drawings
Fig. 1 is the structural representation after set of molds installs.
Fig. 2 is the coronal-plane sectional drawing that holds the wax-pattern piece.
Fig. 3 is the sagittal plane sectional drawing that holds the wax-pattern piece.
Fig. 4 is the structural representation of array hole copper coin.
Fig. 5 is the structural representation of array pin copper coin.
Fig. 6 is the structural representation of L shaped copper backing.
Among the figure, 1, the array hole copper coin; 2, array pin copper coin; 3, L shaped copper backing; 4, array through-hole; 5, array pin; 6, hold wax pan; 7, die cavity.
Embodiment
Below be specific embodiment of the utility model, and in conjunction with the accompanying drawings the technical solution of the utility model be further described, but the utility model is not limited to these embodiment.
The mould of making the one-shot forming organization chip in the utility model comprises array hole copper coin 1, array pin copper coin 2 and two L shaped copper backings 3, array hole copper coin 1 is provided with the array through-hole 4 of n * n rectangle, and the size of array through-hole 4 and arrangement can change as required flexibly.One side of array pin copper coin 2 is provided with and array through-hole 4 array pin 5 one to one, array pin 5 is arranged with array through-hole 4 identical, both the xsect of array pin 5 was identical with the cross section and the degree of depth of array through-hole 4 with height, array hole copper coin 1 and array pin copper coin 2 are rectangle, both width are identical, the length that the length of array pin copper coin 2 slightly is longer than array hole copper coin 1, but easy assembly and disassembly.With 6 * 7 arrays is that example provides reference data: array through-hole 4 numbers of array hole copper coin 1 are 6 * 7; Aperture 2mm, pitch of holes 1mm; Array hole copper coin 1 size is 45 * 30mm
2, thick 4mm.The pin number of array pin copper coin 2 is 6 * 7, pin diameter 2mm, pin spacing 1mm, array pin copper coin 2 sizes 50 * 30mm
2, thick 3mm.As shown in Figure 4 and Figure 5.
As shown in Figure 1, two L shaped copper backings 3 are combined into rectangle, and fill up between array hole copper coin 1 and array pin copper coin 2, and array pin 5 is positioned at two L shaped copper backings 3.Because the existence of L shaped copper backing 3, array pin 5 is plugged in the array through-hole 4, has formed die cavity 7 in array through-hole 4, and as shown in Figure 6, L shaped copper backing 3 is thick to be 1mm, and hem width is 3mm.The long limit of L shaped copper backing 3 is slightly longer than the width of array pin copper coin 2, grow part can conveniently make in the L shaped copper backing 3 of dismounting.
As shown in Figures 2 and 3, also be placed with on the array hole copper coin 1 and hold wax pan 6, this holds the copper cubic frame structure that wax pan 6 is a upper and lower opening, and top is bucket-shaped wide mouthful, and the bottom is the slot structure of tubular.The top of holding wax pan 6 is provided with step-like birdsmouth, and this birdsmouth is corresponding with the plastic embedding frame that pathology department uses, just can support.The slot of bottom is according to the size of array through-hole, bigger it; Consider that section is convenient, height overall should be in 15mm.With 6 * 7 arrays is example: go up the wide 42 * 29mm2 of internal orifice (embedded box is 40 * 28), high 3mm; The high 4mm of rake; Wide 26 * the 24mm2 of bottom internal orifice, high 6mm; The thick about 2mm of wall.
Owing to be lined with L shaped copper backing 3 between array hole copper coin 1 and the array pin copper coin 2, therefore on array through-hole 4, can reserve and the identical hole of L shaped copper backing 3 thickness, these holes are holds wax pan 6, organization chip is positioned over and holds in the wax pan 6, through in the manufacturing process, extract L shaped copper backing 3 out, press array hole copper coin 1, the array pin 5 of array pin copper coin 2 can will hold the organization chip jack-up in the wax pan 6.
Claims (7)
1. mould of making the one-shot forming organization chip, it is characterized in that, this mould comprises array hole copper coin (1), array pin copper coin (2) and two L shaped copper backings (3), array hole copper coin (1) is provided with the array through-hole (4) of rectangle, one side of array pin copper coin (2) is provided with and above-mentioned array through-hole (4) array pin (5) one to one, the xsect of array pin (5) is identical with the cross section and the degree of depth of array through-hole (4) with height, array pin (5) is plugged in the array through-hole (4), above-mentioned two L shaped copper backings (3) are combined into rectangle, and fill up between array hole copper coin (1) and array pin copper coin (2), above-mentioned array pin (5) is positioned at two L shaped copper backings (3).
2. the mould of making one-shot forming organization chip according to claim 1, it is characterized in that, also be placed with on the described array hole copper coin (1) and hold wax pan (6), this holds the copper cubic frame structure that wax pan (6) is a upper and lower opening, top is bucket-shaped wide mouthful, the bottom is the slot structure of tubular, and described top of holding wax pan (6) is provided with step-like birdsmouth.
3. the mould of making one-shot forming organization chip according to claim 1 and 2, it is characterized in that, described array hole copper coin (1) and array pin copper coin (2) are rectangle, and both width are identical, and the length of array pin copper coin (2) slightly is longer than the length of array hole copper coin (1).
4. the mould of making one-shot forming organization chip according to claim 3 is characterized in that, the long limit of described L shaped copper backing (3) is longer than the width of array pin copper coin (2).
5. the mould of making one-shot forming organization chip according to claim 4 is characterized in that, described array pin copper coin (2) size 50 * 30mm
2, thick 3mm, pin spacing are 1mm, the diameter of array pin (5) is 2mm.
6. the mould of making one-shot forming organization chip according to claim 5 is characterized in that, described array hole copper coin (1) size is 45 * 30mm
2, thick 4mm, the aperture 2mm of array through-hole (2), pitch of holes 1mm.
7. the mould of making one-shot forming organization chip according to claim 6 is characterized in that, described L shaped copper backing (3) is thick to be 1mm, and hem width is 3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205880378U CN201885912U (en) | 2010-11-02 | 2010-11-02 | Mold for manufacturing tissue chip with one-step molding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010205880378U CN201885912U (en) | 2010-11-02 | 2010-11-02 | Mold for manufacturing tissue chip with one-step molding |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201885912U true CN201885912U (en) | 2011-06-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010205880378U Expired - Lifetime CN201885912U (en) | 2010-11-02 | 2010-11-02 | Mold for manufacturing tissue chip with one-step molding |
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| CN (1) | CN201885912U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102042922A (en) * | 2010-11-02 | 2011-05-04 | 浙江省台州医院 | Method for making once-forming tissue chip and mold applied to same |
| CN104111184A (en) * | 2013-04-17 | 2014-10-22 | 苏州住友电木有限公司 | Evaluation method of sealing performance, test piece used for evaluation, manufacturing mould and manufacturing method of the test piece |
| CN108801749A (en) * | 2018-05-18 | 2018-11-13 | 郑州大学第附属医院 | A kind of method and device making one-pass molding organization chip acceptor wax block |
-
2010
- 2010-11-02 CN CN2010205880378U patent/CN201885912U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102042922A (en) * | 2010-11-02 | 2011-05-04 | 浙江省台州医院 | Method for making once-forming tissue chip and mold applied to same |
| CN104111184A (en) * | 2013-04-17 | 2014-10-22 | 苏州住友电木有限公司 | Evaluation method of sealing performance, test piece used for evaluation, manufacturing mould and manufacturing method of the test piece |
| CN108801749A (en) * | 2018-05-18 | 2018-11-13 | 郑州大学第附属医院 | A kind of method and device making one-pass molding organization chip acceptor wax block |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| AV01 | Patent right actively abandoned |
Granted publication date: 20110629 Effective date of abandoning: 20120704 |