CN202330106U - Mould for preparing wax mould - Google Patents

Mould for preparing wax mould Download PDF

Info

Publication number
CN202330106U
CN202330106U CN 201120436295 CN201120436295U CN202330106U CN 202330106 U CN202330106 U CN 202330106U CN 201120436295 CN201120436295 CN 201120436295 CN 201120436295 U CN201120436295 U CN 201120436295U CN 202330106 U CN202330106 U CN 202330106U
Authority
CN
China
Prior art keywords
wax
mould
pattern
wax mould
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 201120436295
Other languages
Chinese (zh)
Inventor
侯英勇
石园
何德明
侯君
卢韶华
胡沁
徐晨
刘亚岚
宿杰阿克苏
曾海英
谭云山
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhongshan Hospital Fudan University
Original Assignee
Zhongshan Hospital Fudan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhongshan Hospital Fudan University filed Critical Zhongshan Hospital Fudan University
Priority to CN 201120436295 priority Critical patent/CN202330106U/en
Application granted granted Critical
Publication of CN202330106U publication Critical patent/CN202330106U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Landscapes

  • Molds, Cores, And Manufacturing Methods Thereof (AREA)

Abstract

The utility model relates to a mould for preparing a wax mould. The mould is characterized by comprising a wax mould plate and a wax mould frame. The mould provided by the utility model has the advantages that the wax mould frame and the wax mould plate can be prepared into a paraffin wax tissue chip wax mould after the wax mould frame is embedded with the wax mould plate; the prepared wax mould is easy to separate from the wax mould plate; letters and numbers on the surface of the wax mould are beneficial to subsequent sequential implanting of tissue chip bars; and grid recesses and round recesses are arranged on the surface of the wax mould, and the grid recesses and round recesses are the positions where the tissue chip bars are arranged in the wax mould, so that the implantation of the tissue chip bars is ensured to be neat and ordered. Due to the adoption of the mould for preparing the wax mould, the tissue chip wax mould is prepared conveniently and rapidly by using simple steps; and paraffin wax tissue chips fabricated by using the wax mould are low in price and easier in promotion and popularization.

Description

A kind of mould for preparing wax-pattern
Technical field
The utility model relates to a kind of mould that is used to prepare wax-pattern, is used to prepare the paraffin organization chip wax-pattern, belongs to special biochip technology field.
Background technology
Tissue array technology is the development and the extension of biochip technology, and is the same with cell chip, protein chip, antibody chip, belongs to a kind of special biochip technology.Tissue array technology can be analyzed and researched by the series arrangement of design in advance the clinical tissue sample of dozens of even thousands of Different Individual on a slide, be the analysis tool of a kind of high flux, multisample.Tissue array technology can be analyzed the scientific research personnel simultaneously simultaneously to hundreds of even thousands of kinds of tissue samples, carry out some or a plurality of special genes, or the research of relative expression product.This technology not only can combine with traditional pathological technique, histochemistry and immunohistochemistry technique; Can also combine with technology such as DNA, RNA, protein, antibody, and can on gene, genetic transcription and three levels of correlated expression product biological function, study.The research and development that this learns human genome; Especially to the research of gene and protein and disease relationship; The molecular diagnosis of the checking of disease related gene, the exploitation of novel drugs and screening, disease, aspects such as the tracking of therapeutic process and prognosis have important and practical meanings and vast market prospect.
The organization chip preparation process has following a few step: 1, and the preparation wax-pattern; 2, sampling obtains destination organization; 3, destination organization is seeded in wax-pattern in order; 4, the good wax-pattern afterwards of inoculation is placed the polymerization of polymerization appearance, it is that organization chip has been accomplished that polymerization is accomplished, the research that experimentizes of just can cutting into slices.Yet the wax-pattern of at present a lot of research units mainly is to buy from offshore company, expensive, and about 500 yuan one, therefore, only just there are funds and condition to carry out the correlative study of organization chip in large-scale research unit, be difficult to promote.
Summary of the invention
The purpose of the utility model provides a kind of mould that can prepare the paraffin organization chip wax-pattern easily, and the wax-pattern for preparing can be used for making organization chip.
In order to achieve the above object, the utility model provides a kind of mould for preparing wax-pattern, it is characterized in that, comprises wax-pattern frame and wax template.
Preferably; The central authorities of described wax template are provided with a rectangular region that is surrounded by four first grooves; First groove can be chimeric with the wax-pattern frame; Be provided with at least one transverse concave groove and at least one longitudinal fluting in the rectangular region, transverse concave groove and longitudinal fluting are divided into a plurality of convexities with described rectangular region, and described bump array is latticed.
Preferably, the length of described wax template is 60cm-70cm, and wide is 30cm-40cm, and thick is 0.5cm-1cm.
Preferably, the length of described rectangular region is 30cm-40 cm, and wide is 15cm-25 cm.
Preferably, the wide of described first groove is 0.2mm-0.4 mm, is 0.1 mm deeply.
Preferably, described transverse concave groove has the 4-10 bar, and longitudinal fluting has the 6-15 bar, protruding total 24-150.
Preferably, the length of described convexity is 1mm-3 mm, and wide is 1mm-3 mm.
Preferably; The described raised surface except that first that is arranged in horizontal first row is carved with mirror image numeral or mirror image letter depression; The raised surface except that first that is arranged in vertical first row is carved with mirror image letter or mirror image numeral depression, and remaining raised surface is provided with a circular protrusions.
More preferably, the diameter of described circular protrusions is 1mm.
Preferably, the length of described wax-pattern frame is 30cm-40cm, and wide is 15cm-25cm, and height is 10cm-15cm, and wall thickness is 0.2mm-0.4mm.
The utility model has the advantages that:
1, wax-pattern frame and wax template can prepare the paraffin organization chip wax-pattern after chimeric, and the wax-pattern and the wax template that make are easily separated;
2, the letter on wax-pattern surface and digital helps follow-up orderly plantation organization chip bar;
3, there are grid depression and circular depressed in the wax-pattern surface, helps the position of standard organization chip bar in wax-pattern, makes the plantation of organization chip bar neat and orderly;
4, adopt this Wax mold to prepare the organization chip wax-pattern, step is simple, and is convenient and swift;
5,The paraffin organization chip that adopts wax-pattern to make is cheap, more is prone to penetration and promotion.
Description of drawings
Fig. 1 is the mould structure synoptic diagram of preparation wax-pattern;
Fig. 2 is a wax-pattern mount structure synoptic diagram;
Fig. 3 is the wax template structural representation;
Fig. 4 is the mould that the paraffin of thawing is housed;
Fig. 5 is the structural representation of wax-pattern;
Fig. 6 is the process synoptic diagram of inoculation organization chip on wax-pattern;
Fig. 7 is seeded in the synoptic diagram on the wax-pattern for strip of tissue;
Fig. 8 is for accomplishing whole postvaccinal wax-pattern synoptic diagram;
Fig. 9 is the synoptic diagram of wax-pattern after the finishing polymerization.
Embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is described further.
Embodiment
As shown in Figure 1, be the mould structure synoptic diagram of preparation wax-pattern, the mould of described preparation wax-pattern comprises wax-pattern frame 1 and wax template 2.As shown in Figure 2, be wax-pattern mount structure synoptic diagram, described wax-pattern frame 1 is square, and it is long to be 40cm, and wide is 20cm, and height is 12cm, wall thickness is 0.3mm.
As shown in Figure 3, be the wax template structural representation, described wax template 2 is square, and it is long to be 65cm, and wide is 35cm, and thick is 0.8cm.The central authorities of described wax template 2 are provided with a rectangular region that is surrounded by four first grooves, and the length of described rectangular region is 39.4cm, and wide is 19.4cm.The wide of described first groove is 0.3mm, is 0.1 mm deeply.First groove can be chimeric with the wax-pattern frame, is provided with 8 transverse concave grooves and 11 longitudinal flutings in the rectangular region, and transverse concave groove and longitudinal fluting are divided into 108 convexities with described rectangular region, and described bump array is latticed.The length of described convexity is 2mm, and wide is 2 mm.The described raised surface except that first that is arranged in horizontal first row is carved with mirror image numeral depression, and the raised surface except that first that is arranged in vertical first row is carved with mirror image letter depression, and remaining raised surface is provided with a circular protrusions.The diameter of described circular protrusions is 1mm.
During use, wax-pattern frame 1 and wax template 2 is chimeric, again the paraffin 3 that melts is poured into wax-pattern frame 1, as shown in Figure 4; Be the mould of paraffin that thawing is housed, treat the paraffin cooled and solidified after, separate wax-pattern frame 1 and wax template 2; Wax-pattern 4 is processed, and is as shown in Figure 5, is the structural representation of wax-pattern.With the circular depressed place inserting needle punching of perforating needle 5 in wax-pattern 4 grids depression, as shown in Figure 6, be the process synoptic diagram of inoculation organization chip on wax-pattern; According to numeral on the wax-pattern 4 and letter insert organization chip bar 6 smoothly successively again, as shown in Figure 7, for strip of tissue is seeded in the synoptic diagram on the wax-pattern; Up to accomplishing complete organization chip 7, at last organization chip 7 is separated from wax-pattern frame 1, as shown in Figure 8; For accomplishing whole postvaccinal wax-pattern synoptic diagram,, obtain the smooth organization chip of polymerization 8 through polymerization process; As shown in Figure 9, be the synoptic diagram of wax-pattern after the finishing polymerization, just can cut into slices afterwards for follow-up study.

Claims (10)

1. a mould for preparing wax-pattern is characterized in that, comprises wax-pattern frame (1) and wax template (2).
2. the mould of preparation wax-pattern as claimed in claim 1; It is characterized in that; The central authorities of described wax template (2) are provided with a rectangular region that is surrounded by four first grooves, and first groove can be chimeric with the wax-pattern frame, are provided with at least one transverse concave groove and at least one longitudinal fluting in the rectangular region; Transverse concave groove and longitudinal fluting are divided into a plurality of convexities with described rectangular region, and described bump array is latticed.
3. the mould of preparation wax-pattern as claimed in claim 2 is characterized in that, described wax template (2) is square, and it is long to be 60cm-70cm, and wide is 30cm-40cm, and thick is 0.5cm-1cm.
4. the mould of preparation wax-pattern as claimed in claim 2 is characterized in that, the length of described rectangular region is 30cm-40 cm, and wide is 15cm-25 cm.
5. the mould of preparation wax-pattern as claimed in claim 2 is characterized in that, the wide of described first groove is 0.2mm-0.4 mm, is 0.1 mm deeply.
6. the mould of preparation wax-pattern as claimed in claim 2 is characterized in that, described transverse concave groove has the 4-10 bar, and longitudinal fluting has the 6-15 bar, protruding total 24-150.
7. the mould of preparation wax-pattern as claimed in claim 2 is characterized in that, the length of described convexity is 1mm-3 mm, and wide is 1mm-3 mm.
8. the mould of preparation wax-pattern as claimed in claim 2; It is characterized in that; The described raised surface except that first that is arranged in horizontal first row is carved with mirror image numeral or mirror image letter depression; The raised surface except that first that is arranged in vertical first row is carved with mirror image letter or mirror image numeral depression, and remaining raised surface is provided with a circular protrusions.
9. the mould of preparation wax-pattern as claimed in claim 8 is characterized in that, the diameter of described circular protrusions is 1mm.
10. the mould of preparation wax-pattern as claimed in claim 1 is characterized in that, described wax-pattern frame (1) is square, and it is long to be 30cm-40cm, and wide is 15cm-25cm, and height is 10cm-15cm, and wall thickness is 0.2mm-0.4mm.
CN 201120436295 2011-11-07 2011-11-07 Mould for preparing wax mould Expired - Fee Related CN202330106U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120436295 CN202330106U (en) 2011-11-07 2011-11-07 Mould for preparing wax mould

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201120436295 CN202330106U (en) 2011-11-07 2011-11-07 Mould for preparing wax mould

Publications (1)

Publication Number Publication Date
CN202330106U true CN202330106U (en) 2012-07-11

Family

ID=46442084

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201120436295 Expired - Fee Related CN202330106U (en) 2011-11-07 2011-11-07 Mould for preparing wax mould

Country Status (1)

Country Link
CN (1) CN202330106U (en)

Similar Documents

Publication Publication Date Title
CN102719352B (en) Cell chip slide for preparing microarray cell chips and preparation method
SCHÖNSWETTER et al. Disjunctions in relict alpine plants: phylogeography of Androsace brevis and A. wulfeniana (Primulaceae)
US20110046017A1 (en) Mold of recipient block and usage thereof
CN101694429A (en) Method for embedding wax tissue chip
CN106255744A (en) Culture dish
CN103695557A (en) Gingko karyotype analysis method based on stem tips
CN102998153A (en) Method for making tissue chip based on one-step molding
WO2001073134A3 (en) Gene profiling arrays
CN201583439U (en) Petrolin tissue chip paraffin block manufacture equipment
CN202330106U (en) Mould for preparing wax mould
CN203794894U (en) Culture dish special for micro-injection of zebrafish embryos
CN102042922B (en) Method for making once-forming tissue chip and mold applied to same
CN110146325B (en) Multifunctional tissue section die
CN101556224A (en) Manufacturing method of paraffin tissue chip
CN201885912U (en) Mold for manufacturing tissue chip with one-step molding
CN204265761U (en) cell slide culture apparatus
Nordby Light microscopy of meiotic zoosporogenesis and mitotic gametogenesis in Ulva mutabilis Føyn
CN216771254U (en) Plant microarray paraffin chip preparation device
CN202453243U (en) Paraffin embedding tool for fish tissue
CN205038401U (en) Special slide glass convenient to under a microscope observes cell slide
CN205133606U (en) Micro - sampling device of fence formula
CN100587451C (en) Method for trapping tissue special region and special equipment
CN203337665U (en) Die for preparing cell tissue blocks
CN208420502U (en) A kind of device making one-pass molding organization chip acceptor wax block
CN105296469A (en) Application of plant leaf grinding device for high-throughput rapid DNA extraction

Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120711

Termination date: 20171107

CF01 Termination of patent right due to non-payment of annual fee