CN216771254U - Plant microarray paraffin chip preparation device - Google Patents
Plant microarray paraffin chip preparation device Download PDFInfo
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- CN216771254U CN216771254U CN202220038551.7U CN202220038551U CN216771254U CN 216771254 U CN216771254 U CN 216771254U CN 202220038551 U CN202220038551 U CN 202220038551U CN 216771254 U CN216771254 U CN 216771254U
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Abstract
The utility model discloses a preparation device of a plant microarray paraffin chip. The device includes sample anchor plate and side fixed knot, and sample anchor plate includes roof, last limiting plate, lower limiting plate and bottom plate from the top down in proper order. The utility model can be used for realizing the integral dehydration and transparent embedding of multiple samples, and the array distribution of the samples is convenient for the later integral block repairing and slicing to obtain the matrix paraffin slice. The utility model not only avoids the repeated operations of dehydration, transparence, wax infiltration and the like to each material in the sample preparation process of large-batch paraffin section, but also can realize the synchronous block trimming and section of multiple samples. Compared with the prior art, the time cost from sample preparation to sheet forming of large-batch samples is greatly saved, the use of a large amount of consumables such as centrifuge tubes and glass slides is reduced, and the experimental operation efficiency is greatly improved.
Description
Technical Field
The utility model belongs to the technical field of tissue chips, and particularly relates to a preparation device of a plant microarray paraffin chip.
Background
Paraffin sections are a basic experimental method for observing the microstructure of a tissue in cell biology, and are widely applied to human, animal and plant research. The traditional paraffin section method at present cannot be efficiently carried out in batch in the field of plant science research due to complicated operation steps.
For plant samples, when a large number of samples are dehydrated, transparent, waxed and the like in the traditional method, different reagents of different samples need to be replaced one by one. The later embedding, blocking and slicing processes also require separate separation of different samples and slice sticking respectively. These processes are time consuming and labor intensive, greatly delaying the progress of the experiment. At present, although some automatic instruments or convenient processing devices designed for the early sample preparation process exist, the sample preparation efficiency is obviously improved, but the practicability still needs to be improved. The plant samples need to be separated one by one in the steps of embedding, block trimming, slicing and the like, which is also a key problem for limiting the efficiency of paraffin slicing, and the existing instrument or device cannot solve the problem.
In the existing equipment, aiming at the preparation of tissue chips, more tissue chips related to animal tissues or cell layers are mostly based on the design principle that different samples are punctured and cored through a hollow column, and then the core is filled into a mould with a micro-array small hole and then sliced to obtain the micro-array paraffin chip. These devices are not useful for the preparation of plant tissue chips due to differences in the volume and structure of animal and plant samples.
SUMMERY OF THE UTILITY MODEL
Technical problem to be solved
The utility model aims to provide a plant microarray paraffin chip preparation device which is small in size, simple and convenient to operate and capable of greatly improving experimental efficiency.
(II) technical scheme
In order to solve the problems, the utility model provides the following technical scheme, and provides a plant microarray paraffin chip preparation device, which comprises the following specific steps.
A plant microarray paraffin chip preparation device is characterized by comprising a sample anchor plate and a lateral fixing buckle; the sample anchor plate comprises a top plate (1), an upper limiting plate (5), a lower limiting plate (9) and a bottom plate (10) from top to bottom in sequence; the top plate (1) and the upper limiting plate (5) are attached to each other, the lower limiting plate (9) and the bottom plate (10) are attached to each other, and the top plate (1) and the bottom plate (10) are used for preventing a sample from falling; the upper limiting plate (5) and the lower limiting plate (9) are parallel to each other but not attached to each other, and are separated by a proper distance for the sample to be inserted and placed; the upper limiting plate (5) and the lower limiting plate (9) are both provided with anchoring holes (6) distributed in an array manner, the anchoring holes (6) of the upper limiting plate and the lower limiting plate are located at the same relative position, and the upper anchoring hole (6) and the lower anchoring hole (6) realize position limitation on a single sample; two side fixing buckles are arranged on the left side and the right side of the sample anchoring plate respectively when the sample anchoring plate is fixed; the left and right parts of the top plate (1), the upper limiting plate (5), the lower limiting plate (9) and the bottom plate (10) are provided with side wing protrusions, the side wing protrusions and the side fixing buckles are matched with each other, and fixing and dismounting of the whole sample anchoring plate are achieved.
Preferably, the lateral fixing buckle (13) is provided with an upper rectangular hole and a lower rectangular hole which are consistent in size, the width of each rectangular hole is consistent with the protruding width of the side wings of the limiting plate and the bottom plate, the height difference of the top and the bottom in each hole of the upper rectangular hole (14) is the sum of the thicknesses of the upper limiting plate (5) and the top plate (1), and the height difference of the top and the bottom in each hole of the lower rectangular hole (15) is the sum of the thicknesses of the lower limiting plate (9) and the bottom plate (10).
Preferably, the top edge of the upper rectangular hole (14) of the side fixing buckle (13) is provided with a top edge notch (16), so that the side wing protrusion (4) of the top plate can be placed from the upper side and then horizontally slide, and the clamping and fixing are realized.
Preferably, the top plate (1) and the bottom plate (10) are designed in a matrix window format, and the middle main body part is a frame strip which is intersected horizontally and vertically; in the process of manufacturing the paraffin embedding block, the positions of the grid points of the intersected transverse and vertical frame strips correspond to the positions of the anchoring holes of the upper limiting plate and the lower limiting plate one by one, so that samples are prevented from falling out of the anchoring holes of the upper limiting plate and the lower limiting plate.
Preferably, the right lower sides of the top plate (1), the upper limiting plate (5), the lower limiting plate (9) and the bottom plate (10) are all provided with oblique angles, so that the front and the back of each part can be conveniently and quickly identified and aligned.
Preferably, the central main body area of the top plate (1), the upper limiting plate (5), the lower limiting plate (9) and the bottom plate (10) are all 24.6 × 30 mm in size, the thickness of the top plate (1) and the bottom plate (10) is 1 mm, the thickness of the upper limiting plate (5) and the lower limiting plate (9) is 3 mm, the height interval between the upper limiting plate (5) and the lower limiting plate (9) is 6 mm, and the inner diameter of the anchoring hole (6) is 1.8 mm.
Preferably, the anchoring holes (6) of the upper limiting plate (5) and the lower limiting plate (9) are respectively provided with 36, and a 6 x 6 matrix design is adopted.
When the plant microarray paraffin chip preparation device is used, the preparation method specifically comprises the following steps:
s1, attaching the lower limiting plate and the bottom plate, and inserting the side wings of the lower limiting plate and the bottom plate into the lower rectangular holes of the lateral fixing buckles in a protruding manner to realize alignment and fixation of the lower limiting plate and the bottom plate;
s2, inserting the side wing of the upper limiting plate into the upper rectangular hole of the lateral fixing buckle in a protruding manner, and aligning the array anchoring holes of the upper limiting plate and the lower limiting plate one by one;
s3, inserting a plurality of plant samples into the anchoring holes of the lower limiting plate from top to bottom one by one through the array-type anchoring holes of the upper limiting plate, inserting one plant sample into each anchoring hole, and enabling all the plant samples to be vertically placed;
s4, placing the side wing of the top plate into the notch at the top edge of the upper rectangular hole in a protruding manner, and then sliding into the upper rectangular hole along the horizontal direction to realize the alignment and fixation of the upper limiting plate and the top plate;
s5, carrying out experimental treatment on the plant microarray paraffin section device filled with the plant sample, and then embedding the whole device; cutting off redundant paraffin after the paraffin is completely solidified, and then flattening the side surface of the interlayer wax block of the limiting plate until the side surface of the interlayer wax block is flush with the side edge of the limiting plate to obtain a paraffin embedded block;
and S6, removing the side wing fixing buckle of the paraffin embedding block, removing the top plate and the upper limiting plate to expose the interlayer wax block of the limiting plate, and repairing and flattening the surface to obtain the embedding block to be sliced for subsequent slicing treatment.
Preferably, the experimental treatment of step S5 includes one or more of dehydration, transparentization, and wax impregnation.
Preferably, the type of plant sample is an elongated-like tissue, including but not limited to roots, stems, and leaves.
(III) advantageous effects
The plant microarray paraffin chip preparation device provided by the utility model limits the two ends of a sample, and then the side wing bulges of the sample anchoring plate are embedded through the rectangular square holes of the side fixing buckles, so that the vertical separation and the left and right fixation are realized. Compared with the prior equipment, the utility model has the following obvious and positive technical effects:
firstly, compared with the existing equipment, the whole volume of the device is lighter and smaller, and a plurality of devices are convenient to use simultaneously to carry out a large batch paraffin section experiment.
Secondly, the device is hollow and porous, so that the state of the sample in the experimental process can be conveniently observed, the sample can fully contact with the reagent in the subsequent dehydration, transparence, wax infiltration and embedding processes, and the reagent replacement process is more thorough and convenient.
Thirdly, after embedding is completed, the existing equipment needs to separate samples in an embedding block one by one and then make block trimming slices, anchoring holes of the device are distributed in an array mode, the samples can be vertically anchored in the device in an independent and orderly mode, and the corresponding samples can be confirmed through the positions of the anchoring holes, so that the whole embedding block can be trimmed and sliced, a large amount of time for trimming the block and slicing is saved, and the experimental efficiency is remarkably improved.
Fourthly, the device is made of recyclable materials, the preparation of the microarray paraffin chip is realized, and compared with the existing equipment, the device has less consumption of consumables such as centrifuge tubes, glass slides and the like, is an innovation in the preparation of the plant paraffin chip, and has remarkable and positive economic and social benefits.
Drawings
FIG. 1 is a schematic structural diagram of a plant microarray paraffin chip preparation device
FIG. 2 is a front view of a plant microarray paraffin chip preparation apparatus (with sample)
FIG. 3 is a plan view of a plant microarray paraffin chip preparation apparatus
Description of reference numerals: 1. a top plate; 2. an upper limiting plate; 3. frame strips; 4. the side wings of the top plate are protruded; 5. an upper limiting plate; 6. an anchor hole; 7, the side wing of the limiting plate protrudes; 8. limiting plate mold fixing holes; 9. a lower limiting plate; 10. a base plate; 11. the side wings of the bottom plate are protruded; 12. a bottom plate mold fixing hole; 13. a lateral fixing buckle; 14. An upper rectangular hole; 15. a lower rectangular hole; 16. a top edge notch; 17. a root sample; 18. leaf samples; 19. stem samples.
Detailed Description
The utility model is further explained below with reference to the drawings and the embodiments.
Fig. 1-3 show the plant microarray paraffin chip preparation device provided by the utility model, the array anchoring holes are arranged in a 6 × 6 way, the inner diameter is 1.8 mm, the hole edge distance is 2 mm, the connecting lines of four corners of the anchoring hole array form a square, the side length is 20.8 mm, and is slightly smaller than the wide side of the glass slide. Before using, go up the limiting plate earlier, lower limiting plate, the bottom plate passes through the flank protrusion and detains cooperation fixing device with the side is fixed, then place the device in the plate that is equipped with stationary liquid, insert the sample in the corresponding anchoring hole of lower limiting plate from last anchoring hole down through upper limiting plate in order again, the sample is placed and is accomplished the back, put into the roof side flank protrusion from the fixed topside breach of detaining of side, again along the horizontal direction downthehole of rectangle in the slip, realize the alignment and the fixed of upper limiting plate and roof, accomplish the anchoring process of sample. In addition, metal wires can be inserted into the corresponding die fixing holes of the upper limiting plate, the lower limiting plate and the bottom plate to serve as lifting rings, so that the device can be conveniently transferred among dehydrating agents, transparent agents and wax cup containers with different gradients step by step. After embedding is finished, the wax block outside the three-dimensional space where the device is located is cut off as far as possible until the lateral fixing buckles, the lateral protrusions, the upper surface of the top plate, the lower surface of the bottom plate and the lateral sides of the anchoring plate are exposed, and then the left lateral fixing buckle, the right lateral fixing buckle, the top plate and the upper limiting plate are sequentially removed. When the upper limiting plate is disassembled, the upper surface of the upper limiting plate can be heated for a short time at 60 ℃, so that wax in the anchoring hole of the upper limiting plate is slightly melted, local samples in the anchoring hole are loosened, and the surface of a sandwich wax block in the middle of the limiting plate is relatively flat after the upper limiting plate is disassembled. And in the block trimming process, trimming off redundant wax blocks to ensure that the maximum width of the plane rectangle where the sample is positioned does not exceed the wide edge of the glass slide. And finally, slicing and performing subsequent operation to finish the preparation of the plant microarray paraffin chip.
The specific examples described in the application are only illustrative of the spirit of the utility model. Various modifications, additions and substitutions of types may be made by those skilled in the art without departing from the spirit of the utility model or exceeding the scope of the utility model as defined in the accompanying claims.
Claims (7)
1. A plant microarray paraffin chip preparation device is characterized by comprising a sample anchor plate and a lateral fixing buckle; the sample anchor plate comprises a top plate (1), an upper limiting plate (5), a lower limiting plate (9) and a bottom plate (10) from top to bottom in sequence; the top plate (1) and the upper limiting plate (5) are attached to each other, the lower limiting plate (9) and the bottom plate (10) are attached to each other, and the top plate (1) and the bottom plate (10) are used for preventing a sample from falling; the upper limiting plate (5) and the lower limiting plate (9) are parallel to each other but not attached to each other, and are separated by a proper distance for the sample to be inserted and placed; the upper limiting plate (5) and the lower limiting plate (9) are both provided with anchoring holes (6) distributed in an array manner, the anchoring holes (6) of the upper limiting plate and the anchoring holes (6) of the lower limiting plate are located at the same relative position, and the upper anchoring hole and the lower anchoring hole (6) realize position limitation on a single sample; two side fixing buckles are arranged on the left side and the right side of the sample anchoring plate respectively when the sample anchoring plate is fixed; the left and right parts of the top plate (1), the upper limiting plate (5), the lower limiting plate (9) and the bottom plate (10) are provided with side wing protrusions, the side wing protrusions and the side fixing buckles are matched with each other, and fixing and dismounting of the whole sample anchoring plate are achieved.
2. The plant microarray paraffin chip preparation device according to claim 1, wherein the side fixing buckle (13) is provided with two rectangular holes with the same size, the width of the rectangular hole is the same as the protruding width of the lateral wings of the limiting plate and the bottom plate, the height difference of the top and bottom in the hole of the upper rectangular hole (14) is the sum of the thicknesses of the upper limiting plate (5) and the top plate (1), and the height difference of the top and bottom in the hole of the lower rectangular hole (15) is the sum of the thicknesses of the lower limiting plate (9) and the bottom plate (10).
3. The device for preparing the plant microarray paraffin chip according to claim 2, wherein the top edge of the upper rectangular hole (14) of the side fixing buckle (13) is provided with a top edge notch (16) which is convenient for the side wing protrusion (4) of the top plate to be placed from the upper part and then horizontally slide to realize clamping and fixing.
4. The plant microarray paraffin chip preparation device according to claim 1, characterized in that the top plate (1) and the bottom plate (10) are designed in a matrix window format, and the middle main body part is a frame strip (3) which is intersected horizontally and vertically; in the process of manufacturing the paraffin embedding block, the crossed lattice point positions of the transverse and vertical frame strips (3) correspond to the anchoring hole positions of the upper limiting plate and the lower limiting plate one by one, so that samples are prevented from falling out of the anchoring holes of the upper limiting plate and the lower limiting plate.
5. The apparatus for preparing a paraffin chip for a plant microarray as claimed in claim 1, wherein the top plate (1), the upper limiting plate (5), the lower limiting plate (9) and the bottom plate (10) are provided with chamfered corners at the lower right side to facilitate the rapid recognition and alignment of the front and back sides of each member.
6. The apparatus for preparing a paraffin chip for plant microarray according to claim 1, wherein the central body region of the top plate (1), the upper limiting plate (5), the lower limiting plate (9) and the bottom plate (10) are all 24.6 x 30 mm in size, the thickness of the top plate (1) and the bottom plate (10) is 1 mm, the thickness of the upper limiting plate (5) and the lower limiting plate (9) is 3 mm, the height interval between the upper limiting plate (5) and the lower limiting plate (9) is 6 mm, and the inner diameter of the anchoring hole (6) is 1.8 mm.
7. The apparatus for preparing a plant microarray paraffin chip according to claim 1, wherein the anchoring holes (6) of the upper and lower limiting plates (5, 9) are 36 each, and are designed in a 6 x 6 matrix.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220038551.7U CN216771254U (en) | 2022-01-02 | 2022-01-02 | Plant microarray paraffin chip preparation device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202220038551.7U CN216771254U (en) | 2022-01-02 | 2022-01-02 | Plant microarray paraffin chip preparation device |
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| CN216771254U true CN216771254U (en) | 2022-06-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114295449A (en) * | 2022-01-02 | 2022-04-08 | 华中农业大学 | Plant microarray paraffin chip preparation device and method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114295449A (en) * | 2022-01-02 | 2022-04-08 | 华中农业大学 | Plant microarray paraffin chip preparation device and method |
| CN114295449B (en) * | 2022-01-02 | 2024-04-05 | 华中农业大学 | Plant microarray paraffin chip preparation device and method |
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