CN207964652U - A kind of experimental provision being carried out at the same time zebrafish embryo gene sequencing and Phenotypic Observation - Google Patents

Abstract

The utility model discloses a kind of experimental provisions being carried out at the same time zebrafish embryo gene sequencing and Phenotypic Observation, including hole plate body, it is provided in the plate body of hole along horizontal and vertical in the reagent wells of array distribution, the hole plate body is configured with groove top lid processed and cutting head cover；The first top plug is provided on the bottom surface of the groove top lid processed, each first top is provided with identical frid processed beyond the Great Wall；The second top plug is provided on the bottom surface of the cutting head cover, each second top is provided with beyond the Great Wall identical cuts sample knife；Longitudinal perpendicular of the described lateral perpendicular cut where sample knife where with frid processed is mutually perpendicular to；The vertical extension for cutting sample knife is more than the vertical extension of frid processed.The device of the utility model simply can accurately realize the gene sequencing and Phenotypic Observation of zebrafish embryo, and a kind of experimental provision of simple possible is provided for zebra fish research.

Description

A kind of experimental provision being carried out at the same time zebrafish embryo gene sequencing and Phenotypic Observation

Technical field

The utility model belongs to biomedical sector, is related to zebra fish, and in particular to one kind being carried out at the same time zebrafish embryo The experimental provision of gene sequencing and Phenotypic Observation.

Background technology

Zebra fish as a kind of emerging animal model, with being easy to, express, the embryonic period, embryonic phase transparent live body that is easy to is seen by controlling gene Examine, for observation embryo's number it is more the features such as.CRISPR is emerging in recent years gene editing technology, it is drawn by gRNA It leads, is operated using Cas9 albumen, to be cut off to plurality of target cell DNA.Multinomial research in recent years is by CRISPR technologies For in the gene knockout of zebrafish embryo, it has also become study the important hand of molecule mechanism and pathogenic mechanism in biomedical sector Section.But most of genes have lethal, can not obtain the zebra fish homozygote embryo that can maintain breeding, therefore, Zhi Neng Embryonic period, embryonic phase carries out Phenotypic Observation and sequence verification simultaneously to the embryo of gene knockout heterozygote zebra fish.

Currently, most of study by the way that zebrafish embryo portion of residual cutting tissue is sequenced, and simultaneously to body It observes part.However list embryo's sequencing approach needs accurate operation under the microscope, and it is further still to lack effective device The specification operating process.

Invention content

In view of the deficienciess of the prior art, the purpose of the utility model is to provide one kind being carried out at the same time zebra fish embryo The experimental provision of tire gene sequencing and Phenotypic Observation is carried out at the same time live body embryo for gene knockout Zebrafish Embryo early stage Observation and fish tail sequencing cutting tissue extraction, special experimental facilities is provided for experiment.

In order to solve the above-mentioned technical problem, the utility model adopts the following technical solution is achieved：

A kind of experimental provision being carried out at the same time zebrafish embryo gene sequencing and Phenotypic Observation, including hole plate body, orifice plate It is provided in main body along horizontal and vertical in the reagent wells of array distribution, the hole plate body is configured with groove top lid processed With cutting head cover；

The first top plug that cooperation is corresponded with reagent wells, each first top are provided on the bottom surface of the groove top lid processed It is provided with identical frid processed beyond the Great Wall, frid processed beyond the Great Wall is vertical in the same longitudinal direction positioned at the top of each of same longitudinal row first It is laid in plane；

The second top plug that cooperation is corresponded with reagent wells, each second top are provided on the bottom surface of the cutting head cover Be provided with beyond the Great Wall it is identical cut sample knife, positioned at it is same each of laterally arrange the second top beyond the Great Wall to cut sample knife vertical in the same transverse direction It is laid in plane；

Longitudinal perpendicular of the described lateral perpendicular cut where sample knife where with frid processed is mutually perpendicular to；

The vertical extension for cutting sample knife is more than the vertical extension of frid processed.

The utility model also has following distinguishing feature：

The frid processed is located in longitudinal perpendicular where longitudinal diameter of end face is filled on the first top.

Described cuts on the transverse cuts string parallel with trans D that sample cutter spacing pushes up plug end face in second, described The distance in the top of the transverse cuts chordal distance second plug end face center of circle is that the second top fills in the 30% of diameter.

The top surface quadrangle of the hole plate body is provided with guide pin, and the groove top processed covers to be provided with matches with guide pin The first guide through hole closed is provided with the second guide through hole coordinated with guide pin on the cutting head cover.

The hole plate body is 96 orifice plates.

The utility model compared with prior art, has the following technical effect that：

The device of the utility model simply can accurately realize the gene sequencing and Phenotypic Observation of zebrafish embryo, be zebra Fish research provides a kind of experimental provision of simple possible.

The device of the utility model can once cut multiple zebrafish embryo samples, and each sample can be accurate Ground is cut into five/part and 4/5ths parts, and sample preparation standard increases the accuracy of experiment.

Description of the drawings

Fig. 1 is the external overall structure diagram of the utility model.

Fig. 2 is the structural schematic diagram of hole plate body.

Fig. 3 is the positive structure diagram of frid processed.

Fig. 4 is the left view structural representation of frid processed.

Fig. 5 is the positive structure diagram for cutting sample knife.

Fig. 6 is the left view structural representation for cutting sample knife.

Fig. 7 is the use state diagram of frid processed.

Fig. 8 is the use state diagram for cutting sample knife.

The meaning of each label is in figure：The holes 1- plate body, 2- reagent wells, 3- groove top lids, 4- cut head cover, and 5- is oriented to Pin, 6- agar mediums, 7- grooves；The tops 31- first plug, 32- frids, the first guide through hole of 33-；The tops 41- second plug, 42- Cut sample knife, the second guide through hole of 43-.

Explanation is further explained in detail to the particular content of the utility model with reference to embodiments.

Specific implementation mode

Specific embodiment of the utility model is given below, it should be noted that the utility model is not limited to following tool Body embodiment, all equivalent transformations made on the basis of the technical solutions of the present application each fall within the scope of protection of the utility model.

Embodiment 1：

The present embodiment provides a kind of experimental provision being carried out at the same time zebrafish embryo gene sequencing and Phenotypic Observation, such as Fig. 1 Shown in Fig. 8, including hole plate body 1, be provided in hole plate body 1 along it is horizontal and vertical be in array distribution reagent wells 2, the hole plate body 1 is configured with groove top lid 3 processed and cutting head cover 4；

The first top plug 31, Mei Ge that cooperation is corresponded with reagent wells 2 is provided on the bottom surface of the groove top lid 3 processed It is provided with identical frid processed 32 on one top plug 31, the frid processed 32 on the top of each of same longitudinal row first plug 31 is same It is laid in one longitudinal perpendicular；

The second top plug 41, Mei Ge that cooperation is corresponded with reagent wells 2 is provided on the bottom surface of the cutting head cover 4 Be provided on two top plugs 41 it is identical cut sample knife 42, cut sample knife 42 same on the second top plug 41 positioned at same each of laterally arrange It is laid in one lateral perpendicular；

The lateral perpendicular for cutting 42 place of sample knife and longitudinal perpendicular where frid processed 32 are mutually perpendicular to；

The vertical extension for cutting sample knife 42 is more than the vertical extension of frid 32 processed.

As a kind of preferred embodiment of the present embodiment, frid 32 processed is located at where longitudinal diameter of the first top 31 end faces of plug In longitudinal perpendicular.

As a kind of preferred embodiment of the present embodiment, one and trans D that sample knife 42 fills in 41 end faces positioned at the second top are cut On parallel transverse cuts string, the distance in the transverse cuts chordal distance second top 41 end face centers of circle of plug is that the second top plug 41 is straight The 30% of diameter.Each sample is enable accurately to be cut into five/part and 4/5ths parts.

As a kind of preferred embodiment of the present embodiment, the top surface quadrangle of hole plate body 1 is vertically provided with guide pin 5, described Groove top lid 3 processed on be provided with the first guide through hole 33 coordinated with guide pin 5, be provided with and lead on the cutting head cover 4 The second guide through hole 43 coordinated to pin 5.Convenient for alignment.

As a kind of preferred embodiment of the present embodiment, hole plate body 1 is 96 orifice plates.

The device of the present embodiment is when in use, preferred, in the reagent wells 2 that agar is dissolved by heating to entrance hole plate body 1, uses In forming agar medium 6, it is capped groove top lid 3 processed so that 31 lid of the first top plug closes in reagent wells 2, and frid 32 processed stretches into examination It in agent hole 2 and submerges in agar, waits for that agar cools down for static several minutes, pull out groove top lid 3 processed, can be formed on agar medium 6 Groove 7.

Then by zebrafish embryo according to needed for experiment observe position be positioned on groove 7, be added zebra fish culture solution and Anesthetic carries out corresponding microscopically observation.

Finally capping cutting head cover 4, cuts the fish tail portion of zebra embryo, and cutting head cover 4 is pulled out after the completion of cutting, Tail portion is drawn with sample injector from top blank and be removed tissue, be put into the extraction that genomic DNA is carried out in corresponding container；Zebra The remainder of fish embryo can continue subsequently to cultivate observation.

Claims (5)

1. a kind of experimental provision being carried out at the same time zebrafish embryo gene sequencing and Phenotypic Observation, including hole plate body (1), orifice plate It is provided in main body (1) along horizontal and vertical in the reagent wells (2) of array distribution, which is characterized in that the orifice plate Main body (1) is configured with groove top lid (3) processed and cutting head cover (4)；

The first top plug (31) that cooperation is corresponded with reagent wells (2) is provided on the bottom surface of the groove top lid (3) processed, each It is provided with identical frid processed (32) on first top plug (31), the slot processed being located on the first top plug (31) of each of same longitudinal row Plate (32) is laid in same longitudinal perpendicular；

The second top plug (41) that cooperation is corresponded with reagent wells (2) is provided on the bottom surface of the cutting head cover (4), each Be provided on second top plug (41) it is identical cut sample knife (42), cut sample on the second top plug (41) positioned at same each of laterally arrange Knife (42) is laid in the same lateral perpendicular；

It is described cut sample knife (42) where lateral perpendicular be mutually perpendicular to longitudinal perpendicular where frid processed (32)；

The vertical extension for cutting sample knife (42) is more than the vertical extension of frid processed (32).

2. experimental provision as described in claim 1, which is characterized in that the frid processed (32) is located at the first top and fills in (31) end In longitudinal perpendicular where longitudinal diameter in face.

3. experimental provision as described in claim 1, which is characterized in that the sample knife (42) of cutting fills in (41) end positioned at the second top On one transverse cuts string parallel with trans D in face, the transverse cuts chordal distance second top plug (41) end face center of circle Distance be second top fill in (41) diameter 30%.

4. experimental provision as described in claim 1, which is characterized in that the top surface quadrangle of the hole plate body (1) is vertically set It is equipped with guide pin (5), the first guide through hole (33) with guide pin (5) cooperation is provided on the groove top lid (3) processed, it is described Cutting head cover (4) on be provided with guide pin (5) cooperation the second guide through hole (43).

5. experimental provision as described in claim 1, which is characterized in that the hole plate body (1) is 96 orifice plates.