CN210826165U - Culture bottle sleeve for bacterial culture - Google Patents

Abstract

The utility model discloses a blake bottle sleeve pipe for bacterial culture belongs to microbial cultivation technical field. The culture bottle sleeve comprises a tube body and a filter screen, wherein the top of the tube body is provided with a top opening and a tube edge, and the filter screen is fixed at the bottom opening of the tube body. The utility model discloses an utilize the filter screen to choose fungus device and culture medium separation to can clear up fast and choose the fungus device of choosing that stays after the fungus is cultivateed, the fungus liquid is used in the follow-up experiment of being convenient for.

Description

Culture bottle sleeve for bacterial culture

Technical Field

The utility model belongs to the technical field of the microbial cultivation, in particular to a blake bottle sleeve pipe for bacterial cultivation.

Background

Biological experimental research usually uses a research means of culturing bacteria in culture flasks, and the picking and culturing of monoclonal colonies are the most basic operations for bacterial culture.

In the operation of picking monoclonal colonies, generally, a burned forceps is used to clamp a bacteria picking device such as a sterilization toothpick or a pipette tip, and then the bacteria picking device is used to pick the monoclonal colonies on a solid culture plate, and finally the bacteria picking device is directly placed in a liquid culture medium in a culture bottle for amplification culture.

In the process of implementing the present invention, the inventor finds that the prior art has at least the following problems:

after the culture of the monoclonal bacterial colony is completed, subsequent downstream operations are required, for example, bacteria are collected centrifugally or bacterial liquid DNA is extracted in a small amount directly, and bacteria selecting devices such as toothpicks or pipette tips are arranged in culture bottles, so that the bacteria selecting devices cannot be cleaned quickly, and the bacterial liquid cannot be subjected to the downstream operations directly.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a strain picking device that is used for bacteria cultivation's blake bottle sleeve pipe can clear up fast and choose the fungus to cultivate to stay. The technical scheme is as follows:

the embodiment of the utility model provides a blake bottle sleeve pipe for bacterial culture, the blake bottle sleeve pipe includes body and filter screen, the bottom of body is equipped with the bottom opening, the filter screen sets up the bottom opening part, the top of body is provided with open-top and is used for hanging the pipe edge of establishing on the opening of blake bottle, the pipe edge open-top's outward flange circumference is arranged, open-top passes through the body with the filter screen with the bottom opening intercommunication.

Further, the tube extends outwardly to form a groove for mating with an open edge of the culture bottle, the groove being disposed toward the bottom of the tube body.

Further, the bottom of body is equipped with the carrier ring, the carrier ring is arranged along bottom open-ended inward flange circumference, the filter screen sets up on the carrier ring.

Further, the outer edge of the filter screen is adhered to the bearing ring.

Further, the outer edge of the screen is affixed to the side of the carrier ring facing the top of the tube.

Further, the pipe edge, the pipe body and the bearing ring are all plastic structural members.

Further, the pipe edge, the pipe body and the bearing ring are an integrated structural member.

Further, the wall thickness of body is 1 ~ 2 mm.

Further, the height of the filter screen is 1.5-3.5 cm.

Furthermore, the mesh number of the filter screen is 12-18 meshes.

The embodiment of the utility model provides a beneficial effect that technical scheme brought is:

through setting up the blake bottle sleeve pipe, this blake bottle sleeve pipe includes body and filter screen, and the top of body is provided with open-top and tub edge, and the bottom opening part at the body is fixed to the filter screen. Fix the blake bottle sleeve on the opening of blake bottle through the pipe edge, put the bacterium together with choosing fungus device on the filter screen from the open-top of body, will choose the culture solution keep in touch in fungus device and the blake bottle through the filter screen during bacterial cultivation, after bacterial cultivation is accomplished, take out the blake bottle sleeve, utilize the filter screen to choose fungus device and culture medium separation to can clear up fast and choose the fungus device of choosing that the fungus is cultivateed and is left, consequently, it is more convenient when the fungus liquid is used in follow-up experiment.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic structural view of a culture flask casing according to an embodiment of the present invention;

fig. 2 is an axial cross-sectional view of a culture bottle sleeve according to an embodiment of the present invention.

The symbols in the drawings represent the following meanings:

1-pipe body, 11-bottom opening, 12-top opening, 13-pipe edge, 14-groove, 15-bearing ring and 2-filter screen.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention will be described in further detail below with reference to the accompanying drawings.

For a better understanding of the present invention, the structure of the culture flask casing for bacterial culture is briefly described below:

fig. 1 is the structural schematic diagram of the culture bottle sleeve provided by the embodiment of the present invention, as shown in fig. 1, this culture bottle sleeve includes a tube body 1 and a filter screen 2, the bottom of the tube body 1 is provided with a bottom opening 11, the filter screen 2 is disposed at the bottom opening 11, the top of the tube body 1 is provided with a top opening 12 and a tube edge 13 for hanging on the opening of the culture bottle, the tube edge 13 is circumferentially disposed along the outer edge of the top opening 12, and the top opening 12 is communicated with the bottom opening 11 through the tube body 1 and the filter screen 2.

The embodiment of the utility model provides a through setting up the blake bottle sleeve pipe, this blake bottle sleeve pipe includes body and filter screen, and the top of body is provided with open-top and pipe edge, and the bottom opening part at the body is fixed to the filter screen. Fix the blake bottle sleeve on the opening of blake bottle through the pipe edge, put the bacterium together with choosing fungus device on the filter screen from the open-top of body, will choose the culture solution keep in touch in fungus device and the blake bottle through the filter screen during bacterial cultivation, after bacterial cultivation is accomplished, take out the blake bottle sleeve, utilize the filter screen to choose fungus device and culture medium separation to can clear up fast and choose the fungus device of choosing that the fungus is cultivateed and is left, consequently, it is more convenient when the fungus liquid is used in follow-up experiment.

Fig. 2 is an axial sectional view of a culture bottle sleeve according to an embodiment of the present invention, as shown in fig. 2, the tube edge 13 extends outward to form a groove 14 for matching with an opening edge of a culture bottle, and the groove 14 is disposed toward the bottom of the tube body 1.

In the above embodiment, the culture bottle sleeve is matched with the opening edge of the culture bottle through the groove 14, so that the culture bottle sleeve is convenient to mount and dismount.

Illustratively, the culture bottle sleeves are of different sizes so that the culture bottle sleeves can fit on different culture bottles. For different culture bottles, the culture bottle sleeve is provided with a matched pipe edge 13, so that the culture bottle sleeve is conveniently fixed on the opening of the culture bottle sleeve through the groove 14. The culture bottle sleeve can be respectively suitable for fixing culture bottles such as a deep hole plate, a centrifuge tube, various test tubes, a triangular flask and the like according to different diameters and lengths.

Further, the bottom of the tubular body 1 is provided with a carrier ring 15, the carrier ring 15 being arranged circumferentially along the inner edge of the bottom opening 11, the filter screen 2 being provided on the carrier ring 15.

In the above described embodiment, the carrier ring 15 facilitates mounting and fixing of the screen 2.

Exemplarily, be connected through the mode of fillet between carrier ring 15 and the body 1, this fillet has great radian, can make filter screen 2 spread along the upper surface of carrier ring 15 in the installation, and the installation of the filter screen 2 of being convenient for prevents to produce the clearance between filter screen 2 and the body 1.

Further, the outer edge of the screen 2 is glued to the carrier ring 15.

In the above embodiment, the filter net 2 is fixed on the bearing ring 15 by means of adhesion, so that the filter net 2 can be replaced conveniently.

Illustratively, the filter screen 2 may be affixed to the carrier ring 15 by means of edge banding glue for affixing the filter screen 2 to the carrier ring.

Further, the outer edge of the sieve 2 is glued on the side of the carrying ring 15 facing the top of the tubular body 1.

In the above embodiment, the screen 2 is fixed on the side facing the top of the tubular body 1 to facilitate the load of the load ring 15.

It is easy to understand that, in the process of bacteria culture, the filter screen 2 is in direct contact with the culture medium of the culture bottle, and the filter screen 2 not only can keep the bacteria selecting device in contact with the culture medium, but also can play a role in separating the bacteria selecting device from the culture medium after the bacteria culture is completed. On the one hand, among the bacterial culture process, keep filter screen 2 and culture medium contact and can guarantee that the bacterium of choosing fungus device smuggleing secretly above filter screen 2 can utilize the culture medium to breed, on the other hand, after the bacterial culture, take out the blake bottle sleeve pipe from the opening part of blake bottle, realize choosing fungus device and culture medium separation through filter screen 2.

Further, the rim 13, the body 1 and the ring 15 are all plastic structures.

In the above embodiments, the plastic structural member is more stable and can be effectively subjected to radiation sterilization before use.

The rim 13, body 1 and carrier ring 15 may illustratively be PP plastic.

Further, the pipe rim 13, the pipe body 1 and the bearing ring 15 are an integral structural member.

In the above embodiment, the tube rim 13, the tube body 1 and the bearing ring 15 are an integral structure, which can prevent the components from being separated during use.

Illustratively, the culture flask cannulas may be disposable or may be cleaned and then autoclaved with the culture flasks for reuse.

Optionally, the wall thickness of the pipe body 1 is 1-2 mm.

Preferably, the wall thickness of the tubular body 1 is 1 mm.

In the above embodiment, the thickness of the pipe body 1 is 1-2 mm, so that the structural stability of the pipe body 1 can be ensured.

Preferably, the height of the filter screen 2 is 1.5-3.5 cm.

In above-mentioned embodiment, 2 direction of height of filter screen is the axial of body 1, and 2 highly for 1.5 ~ 3.5cm of filter screen can guarantee that 2 filter screens hold the fungus device of choosing of common size.

Exemplarily, the interval of body 1 and blake bottle bottom is 1 ~ 5mm, can prevent that culture medium and filter screen 2 contact inadequately in the process of cultivateing to influence the cultivation of bacterium.

Optionally, the mesh number of the filter screen 2 is 12-18 meshes.

Preferably, the mesh number of the filter screen 2 is 15 meshes.

In the above embodiment, the mesh number of the filter net 2 is 12 to 18 meshes, which can ensure sufficient contact between the bacteria selecting device and the culture medium and can prevent the bacteria selecting device from passing through the filter net.

The working steps of the utility model are as follows: the sealed culture bottle sleeve with the matched size is sterilized, a certain amount of culture medium is added into the culture bottle, the culture bottle sleeve is fixed on an opening of the culture bottle through a pipe edge 13, a bacterium picking device after bacterium picking and bacteria are placed into the culture bottle sleeve from a top opening 12 of a pipe body 1, the culture bottle is sealed by a cover, and finally the bacteria are cultured under proper conditions. After the culture is finished, the cover is opened and the culture bottle sleeve is taken out.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims (10)

1. The utility model provides a blake bottle sleeve pipe for bacterial culture, characterized in that, the blake bottle sleeve pipe includes body (1) and filter screen (2), the bottom of body (1) is equipped with bottom opening (11), filter screen (2) set up bottom opening (11) department, the top of body (1) is provided with open-top (12) and is used for hanging pipe edge (13) of establishing on the opening of blake bottle, pipe edge (13) are followed the outward flange circumference of open-top (12) is arranged, open-top (12) are passed through body (1) with filter screen (2) with bottom opening (11) intercommunication.

2. A culture flask sleeve according to claim 1, characterized in that the tube rim (13) extends outwards forming a groove (14) for mating with the open edge of the culture flask, the groove (14) being arranged towards the bottom of the tube body (1).

3. The culture flask sleeve according to claim 1, characterized in that the bottom of the tube body (1) is provided with a carrier ring (15), the carrier ring (15) being arranged circumferentially along the inner edge of the bottom opening (11), the sieve (2) being provided on the carrier ring (15).

4. The culture flask sleeve according to claim 3, characterized in that the outer edge of the sieve (2) is glued to the carrier ring (15).

5. The culture flask sleeve as claimed in claim 4, characterized in that the outer edge of the sieve (2) is glued on the side of the carrier ring (15) facing the top of the tube body (1).

6. The culture flask sleeve as claimed in claim 3, wherein the rim (13), the tubular body (1) and the bearing ring (15) are all plastic structural parts.

7. The culture flask sleeve as claimed in claim 6, wherein the tube rim (13), the tube body (1) and the carrier ring (15) are a one-piece structural member.

8. A culture flask sleeve according to any one of claims 1-7, wherein the wall thickness of the tube (1) is 1-2 mm.

9. A culture flask sleeve according to any one of claims 1-7, characterized in that the height of the sieve (2) is 1.5-3.5 cm.

10. A culture flask sleeve according to any one of claims 1-7, wherein the mesh number of the sieve (2) is 12-18 mesh.

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