JPS6019587Y2 - Lou test tube - Google Patents

Description

[Detailed explanation of the idea] The present invention relates to roux test tubes used for culturing microorganisms.

For culturing pathogenic microorganisms and other microorganisms such as Mycobacterium tuberculosis,
Conventionally, a roux test tube 1' with a diameter of about 115 to 1/4 from the tip as shown in FIG. A liquid medium) is placed in the tube, and potato pieces 3 (two-tone potato medium) are placed above the narrow diameter portion for cultivation (two-tone potato medium culture).

As the culture progresses (about 6 weeks), Mycobacterium tuberculosis 4 grows on the potato pieces 3, and a bacterial film 5 is formed on the surface of the medium 2. In order to collect the bacterial film 5, first, as shown in Figure 1, Insert the platinum loop 6 into the roux test tube 1' and pull out the potato pieces 3. As shown in FIG. 'Insert medium 2 into
It was necessary to collect the bacterial membrane 5 on the surface and transfer it to a triangular Kolben 8 for passage as shown in the first drawing.

However, in this series of operations, when removing the potato pieces 3 on which Mycobacterium tuberculosis has grown, Mycobacterium tuberculosis 4 adheres to the inner wall of the Roux test tube 1', and then a platinum loop 6 is inserted and the medium 2 When collecting the bacterial membrane 5 on the surface, the entire platinum loop 6 is placed in the roux test tube 1.
'It becomes contaminated with tuberculosis bacteria4 that adheres to the wall.

Furthermore, the potato pieces 3 that have been removed are also left open on the Petri dish 7, which is very dangerous.

The present invention was made to eliminate the drawbacks of the conventional Roux test tube for culture, and is a Roux test tube aimed at improving safety against tuberculosis infection and simplifying the work procedure for subculture transplantation. This applies to

Embodiments of the present invention will be described below based on the drawings.

Figure 2A shows an embodiment of the present invention, in which the diameter of the roux test tube 1 is made narrower at a point approximately 1/5 to 1/4 of the length from the tip.
The middle part of the roux test tube 1 has a large diameter extending over a required length from the mouth to approximately 115 to 315 mm, and a tip part 9, a middle part 10, and a large diameter part 11 are formed in order from the lower end.

As shown in FIG. 2A, a culture medium 2 is placed in the tip part 9, a potato piece 3 is placed in the middle part 10, and tuberculosis bacteria is cultured. After that, a platinum loop 6 is inserted and the potato piece 3 is stabbed. As shown in the figure, the potato pieces 3 are pulled up to the large diameter portion 11 using the platinum loops 6,
The surface of the potato piece 3 on which the tubercle bacillus 4 is growing is the thick diameter part 1
The potato piece 3 is hooked onto the corner 12 of the large diameter portion 11, and the platinum loop 6 is removed.As shown in FIG. The fungal membrane 5 formed on the surface is collected and transplanted into a triangular Kolben 8 for subculturing as shown in the second drawing.

It should be noted that the Roux test tube of the present invention is not limited to the above-described embodiments, and it goes without saying that various changes can be made without departing from the gist of the present invention.

As described above, the Roux test tube of the present invention exhibits various excellent effects as described below.

(1) The roux test tube has a large diameter section into which the potato pieces with developed tuberculosis bacteria are dropped, so there is no need to remove the potato pieces as in the conventional method, and the large diameter section is directly above the potato juice insertion part. This prevents contamination of the inner wall with Mycobacterium tuberculosis when moving potato pieces.

(11) Since the contamination of the inner wall of the Roux test tube by M. tuberculosis can be kept to a minimum, it is possible to limit the area of M. tuberculosis adhesion to the platinum loop from which the bacterial membrane has been collected to the tip of the loop. Therefore, it is extremely effective in preventing infection.

(iii) Potato pieces with developed tuberculosis bacteria are dropped into the large diameter part and held there, which eliminates the need for the conventional procedure of leaving the extracted potato pieces open on a petri dish, improving safety. do.

(ψ In the passage transplantation operation, the platinum loop can be taken in and out of the Roux test tube only once, instead of twice conventionally.

Therefore, the work procedure can be simplified and work efficiency can be improved.

(V) It can be used not only for culturing Mycobacterium tuberculosis but also for culturing other microorganisms.

[Brief explanation of the drawing]

Figure 1 A 9, C, and 2 are diagrams showing the procedure for subculture transplantation using conventional Roux test tubes. Figure 1 A shows the state in which a platinum loop is inserted. Figure 1 C shows the potato piece pulled out and the platinum loop inserted again. Figure 1 shows the potato piece transplanted into the container for subculture transplantation. Figure 2 Figures A9, C, and II are diagrams showing the procedure for passage transplantation using the Roux test tube of the present invention; Figure 2A is a diagram showing the state in which a platinum loop is inserted; Figure 2 C shows a state in which a potato piece that has been stabbed is pulled up to the large diameter part, hooked on the corner of the large diameter part, and the platinum loop is pulled out. FIG. 2 is a diagram showing a state in which it is transplanted into a container for subculture transplantation. In the figure, 1 is a roux test tube, 2 is a culture medium, and 3 is a potato piece of 1.9
10 indicates a tip portion, 10 indicates an intermediate portion, and 11 indicates a large diameter portion.

Claims (1)

[Scope of utility model registration request] A large diameter portion capable of accommodating a solid medium piece is provided approximately in the longitudinal direction of the tube with one end sealed, a small diameter portion is provided between the large diameter portion and the opposite end, and the large diameter portion and the small diameter portion are provided between the large diameter portion and the opposite end. A roux test tube characterized in that it is configured to be able to hold the solid medium piece between the tube and the tube.