JPS6019587Y2 - Lou test tube - Google Patents

Lou test tube

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Publication number
JPS6019587Y2
JPS6019587Y2 JP1982140339U JP14033982U JPS6019587Y2 JP S6019587 Y2 JPS6019587 Y2 JP S6019587Y2 JP 1982140339 U JP1982140339 U JP 1982140339U JP 14033982 U JP14033982 U JP 14033982U JP S6019587 Y2 JPS6019587 Y2 JP S6019587Y2
Authority
JP
Japan
Prior art keywords
test tube
roux
large diameter
diameter portion
potato
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
JP1982140339U
Other languages
Japanese (ja)
Other versions
JPS5944299U (en
Inventor
茂 井田
Original Assignee
ゼリア新薬工業株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ゼリア新薬工業株式会社 filed Critical ゼリア新薬工業株式会社
Priority to JP1982140339U priority Critical patent/JPS6019587Y2/en
Publication of JPS5944299U publication Critical patent/JPS5944299U/en
Application granted granted Critical
Publication of JPS6019587Y2 publication Critical patent/JPS6019587Y2/en
Expired legal-status Critical Current

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Devices For Use In Laboratory Experiments (AREA)

Description

【考案の詳細な説明】 本考案は微生物の培養に使用するルー試験管に関する。[Detailed explanation of the idea] The present invention relates to roux test tubes used for culturing microorganisms.

病源微生物、その他の微生物例えば結核菌の培養には、
従来第1図イに示すような先端から約115〜1/4の
ところを細径にしたルー試験管1′を用い、該ルー試験
管1′の先端部に培地2(例えばツートン無蛋白合成液
体培地)を入れ、細径部上方に馬鈴薯片3(ツートン馬
鈴薯培地)を入れて培養している(ツートン馬鈴薯培地
培養)。
For culturing pathogenic microorganisms and other microorganisms such as Mycobacterium tuberculosis,
Conventionally, a roux test tube 1' with a diameter of about 115 to 1/4 from the tip as shown in FIG. A liquid medium) is placed in the tube, and potato pieces 3 (two-tone potato medium) are placed above the narrow diameter portion for cultivation (two-tone potato medium culture).

培養が進む(約6週)と馬鈴薯片3に結核菌4が発育し
、培地2表面には菌膜5が形成されるが、該菌膜5を採
取するため先ず第1図口に示すように自白金耳6をルー
試験管1′に挿入し馬鈴薯片3を抜き取り、第1図へに
示すように抜き取った馬鈴薯片3をシャーレ7に移した
後、再び白金耳6をルー試験管1′内に挿入して培地2
表面の菌膜5を採取腰第1図工に示すように継代用の三
角コルベン8に移植する必要があった。
As the culture progresses (about 6 weeks), Mycobacterium tuberculosis 4 grows on the potato pieces 3, and a bacterial film 5 is formed on the surface of the medium 2. In order to collect the bacterial film 5, first, as shown in Figure 1, Insert the platinum loop 6 into the roux test tube 1' and pull out the potato pieces 3. As shown in FIG. 'Insert medium 2 into
It was necessary to collect the bacterial membrane 5 on the surface and transfer it to a triangular Kolben 8 for passage as shown in the first drawing.

しかし、この一連の作業に於ては、結核菌の発育した馬
鈴薯片3を抜き取る時にルー試験管1′の内壁に結核菌
4が付着してしまい、次に白金耳6を挿入して培地2表
面の菌膜5を採取する際、白金耳6全体がルー試験管1
′壁に付着した結核菌4により汚染されてしまう。
However, in this series of operations, when removing the potato pieces 3 on which Mycobacterium tuberculosis has grown, Mycobacterium tuberculosis 4 adheres to the inner wall of the Roux test tube 1', and then a platinum loop 6 is inserted and the medium 2 When collecting the bacterial membrane 5 on the surface, the entire platinum loop 6 is placed in the roux test tube 1.
'It becomes contaminated with tuberculosis bacteria4 that adheres to the wall.

又、抜き取った馬鈴薯片3もシャーレ7上に開放のまま
放置するので大変危険な状態にある。
Furthermore, the potato pieces 3 that have been removed are also left open on the Petri dish 7, which is very dangerous.

本考案は斯かる従来の培養用のルー試験管の欠点を除去
するために為したものであり、結核感染に対する安全性
の向上と継代移植の作業手順の簡素化を目的としたルー
試験管にかかるものである。
The present invention was made to eliminate the drawbacks of the conventional Roux test tube for culture, and is a Roux test tube aimed at improving safety against tuberculosis infection and simplifying the work procedure for subculture transplantation. This applies to

以下、図面に基づいて本考案の実施例を説明する。Embodiments of the present invention will be described below based on the drawings.

第2図イは本考案の一実施例であり、ルー試験管1の先
端から約1/、5〜1/4の長さのところを細径とし、
該ルー試験管1の中程のところを所要の長さく口元から
約115〜315)に亘って大径とし、下端から順次先
端部9、中間部10、大径部11としである。
Figure 2A shows an embodiment of the present invention, in which the diameter of the roux test tube 1 is made narrower at a point approximately 1/5 to 1/4 of the length from the tip.
The middle part of the roux test tube 1 has a large diameter extending over a required length from the mouth to approximately 115 to 315 mm, and a tip part 9, a middle part 10, and a large diameter part 11 are formed in order from the lower end.

第2図イに示すように先端部9に培地2を入れ、中間部
10に馬鈴薯片3を入れて結核菌を培養した後、白金耳
6を挿入して馬鈴薯片3を刺し、第2図口に示すように
白金耳6により馬鈴薯片3を大径部11まで引き上げ、
馬鈴薯片3の結核菌4の発育している面が前記太径部1
1の管壁に当接するよう反転させ、馬鈴薯片3を太径部
11の角12にひっかけて、白金耳6を抜き、第2図へ
に示すように白金耳6を抜き出すことなくそのまま培地
2表面に形成された菌膜5を採取し、第2図工に示すよ
うに継代培養用の三角コルベン8に移植する。
As shown in FIG. 2A, a culture medium 2 is placed in the tip part 9, a potato piece 3 is placed in the middle part 10, and tuberculosis bacteria is cultured. After that, a platinum loop 6 is inserted and the potato piece 3 is stabbed. As shown in the figure, the potato pieces 3 are pulled up to the large diameter portion 11 using the platinum loops 6,
The surface of the potato piece 3 on which the tubercle bacillus 4 is growing is the thick diameter part 1
The potato piece 3 is hooked onto the corner 12 of the large diameter portion 11, and the platinum loop 6 is removed.As shown in FIG. The fungal membrane 5 formed on the surface is collected and transplanted into a triangular Kolben 8 for subculturing as shown in the second drawing.

なお、本考案のルー試験管は上述の実施例のみに限定さ
れるものではなく、本考案の要旨を逸脱しない範囲内に
おいて種々変更を加え得ることは勿論である。
It should be noted that the Roux test tube of the present invention is not limited to the above-described embodiments, and it goes without saying that various changes can be made without departing from the gist of the present invention.

以上述べたように本考案のルー試験管によれば、以下の
如き種々の優れた効果を発揮する。
As described above, the Roux test tube of the present invention exhibits various excellent effects as described below.

(1)ルー試験管に大径部を設け、結核菌の発育した馬
鈴薯片を落し込むようにしたので、従来のように馬鈴薯
片を抜き取る必要がなく且つ大径部は馬鈴薯汁挿入部の
直上にあるため馬鈴薯片の移動に際しても結核菌による
内壁の汚染を防止できる。
(1) The roux test tube has a large diameter section into which the potato pieces with developed tuberculosis bacteria are dropped, so there is no need to remove the potato pieces as in the conventional method, and the large diameter section is directly above the potato juice insertion part. This prevents contamination of the inner wall with Mycobacterium tuberculosis when moving potato pieces.

(11)ルー試験管内壁の結核菌による汚染を最少限の
範囲に止どめることができるので、菌膜を採取した白金
耳の結核菌付着範囲を白金耳の先端に限局することが可
能であり、感染防止対策上極めて有効である。
(11) Since the contamination of the inner wall of the Roux test tube by M. tuberculosis can be kept to a minimum, it is possible to limit the area of M. tuberculosis adhesion to the platinum loop from which the bacterial membrane has been collected to the tip of the loop. Therefore, it is extremely effective in preventing infection.

(iii)結核菌の発育した馬鈴薯片を大径部に落とし
込んで保持するようにしたので、従来のように抜き取っ
た馬鈴薯片をシャーレ上で開放のまま放置する操作が不
要となり、安全性が向上する。
(iii) Potato pieces with developed tuberculosis bacteria are dropped into the large diameter part and held there, which eliminates the need for the conventional procedure of leaving the extracted potato pieces open on a petri dish, improving safety. do.

(ψ 継代移植操作において、ルー試験管への白金耳の
出し入れ回数が、従来2回であったものを1回で済ませ
ることができる。
(ψ In the passage transplantation operation, the platinum loop can be taken in and out of the Roux test tube only once, instead of twice conventionally.

従って、作業手順も簡素化でき、作業性も向上できる。Therefore, the work procedure can be simplified and work efficiency can be improved.

(V) 結核菌の培養ばかりでなく、他の微生物の培
養にも使用できる。
(V) It can be used not only for culturing Mycobacterium tuberculosis but also for culturing other microorganisms.

【図面の簡単な説明】[Brief explanation of the drawing]

第1図イ9口、ハ、二は従来のルー試験管を用いた継代
移植の手順示す図であり第1図イは白金耳を挿入した状
態を示す図、第1図口は馬鈴薯片を引き上げた状態を示
す図、第1図ハは馬鈴薯片を引き抜いた後白金耳を再び
挿入した状態を示す図、第1図工は継代移植用容器に移
植した状態を示す図、第2図イ9口、ハ、二は本考案の
ルー試験管を用いた継代移植の手順を示す図であり、第
2図イは白金耳を挿入した状態を示す図、第2図口は白
金耳に刺した馬鈴薯片を大径部に引き上げ、大径部の角
にひっかけて白金耳を引き抜いた状態の図、第2図ハは
白金耳により菌膜を採取する状態を示す図、第2図工は
継代移植用容器に移植した状態を示す図である。 図中、1はルー試験管、2は培地、3は馬鈴薯片1.9
は先端部、10は中間部、11は大径部を示す。
Figure 1 A 9, C, and 2 are diagrams showing the procedure for subculture transplantation using conventional Roux test tubes. Figure 1 A shows the state in which a platinum loop is inserted. Figure 1 C shows the potato piece pulled out and the platinum loop inserted again. Figure 1 shows the potato piece transplanted into the container for subculture transplantation. Figure 2 Figures A9, C, and II are diagrams showing the procedure for passage transplantation using the Roux test tube of the present invention; Figure 2A is a diagram showing the state in which a platinum loop is inserted; Figure 2 C shows a state in which a potato piece that has been stabbed is pulled up to the large diameter part, hooked on the corner of the large diameter part, and the platinum loop is pulled out. FIG. 2 is a diagram showing a state in which it is transplanted into a container for subculture transplantation. In the figure, 1 is a roux test tube, 2 is a culture medium, and 3 is a potato piece of 1.9
10 indicates a tip portion, 10 indicates an intermediate portion, and 11 indicates a large diameter portion.

Claims (1)

【実用新案登録請求の範囲】[Scope of utility model registration request] 一端を封じた管の長手力向略中夫に固形培地片を収容し
得る大径部を設け、該大径部と対端との間に細径部を設
け、前記大径部と細径部との間に前記固形培地片を保持
し得るよう構成したことを特徴とするルー試験管。
A large diameter portion capable of accommodating a solid medium piece is provided approximately in the longitudinal direction of the tube with one end sealed, a small diameter portion is provided between the large diameter portion and the opposite end, and the large diameter portion and the small diameter portion are provided between the large diameter portion and the opposite end. A roux test tube characterized in that it is configured to be able to hold the solid medium piece between the tube and the tube.
JP1982140339U 1982-09-16 1982-09-16 Lou test tube Expired JPS6019587Y2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP1982140339U JPS6019587Y2 (en) 1982-09-16 1982-09-16 Lou test tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1982140339U JPS6019587Y2 (en) 1982-09-16 1982-09-16 Lou test tube

Publications (2)

Publication Number Publication Date
JPS5944299U JPS5944299U (en) 1984-03-23
JPS6019587Y2 true JPS6019587Y2 (en) 1985-06-12

Family

ID=30314247

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1982140339U Expired JPS6019587Y2 (en) 1982-09-16 1982-09-16 Lou test tube

Country Status (1)

Country Link
JP (1) JPS6019587Y2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5439327A (en) * 1977-09-05 1979-03-26 Kawasaki Steel Co Apparatus for electrolytically treating strips
JPS55148798A (en) * 1979-05-10 1980-11-19 Nippon Kokan Kk <Nkk> Manufacture of zinc elecroplated steel sheet
JPS5615889A (en) * 1979-07-19 1981-02-16 Tadashi Niimi Sewage purifying apparatus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5439327A (en) * 1977-09-05 1979-03-26 Kawasaki Steel Co Apparatus for electrolytically treating strips
JPS55148798A (en) * 1979-05-10 1980-11-19 Nippon Kokan Kk <Nkk> Manufacture of zinc elecroplated steel sheet
JPS5615889A (en) * 1979-07-19 1981-02-16 Tadashi Niimi Sewage purifying apparatus

Also Published As

Publication number Publication date
JPS5944299U (en) 1984-03-23

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