CN210803043U - Pig respiratory track microorganism aerosol quantitative collection system - Google Patents
Pig respiratory track microorganism aerosol quantitative collection system Download PDFInfo
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- CN210803043U CN210803043U CN201921742886.1U CN201921742886U CN210803043U CN 210803043 U CN210803043 U CN 210803043U CN 201921742886 U CN201921742886 U CN 201921742886U CN 210803043 U CN210803043 U CN 210803043U
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Abstract
The utility model provides a pig respiratory track microorganism aerosol quantitative acquisition device relates to poultry animal doctor technical field. The pig respiratory tract microorganism aerosol quantitative acquisition device comprises a pig mouth binding sleeve, an air inlet unit and an air outlet unit; the air inlet unit comprises an air filter, an air compressor and a valve which are sequentially connected through an air inlet pipe; the air outlet unit comprises a negative pressure air extractor, a trapping bottle and a valve which are sequentially connected through an air outlet pipe; the tail ends of the air outlet pipe and the air inlet pipe are respectively inserted into the positions of the pig mouth binding sleeve corresponding to the pig nostrils. The utility model discloses collection system simple structure can avoid the interference of original microorganism in the air, also can avoid the gas of exhalation to get into the interference that the respiratory tract caused by collecting after once more in the collection process, consequently can be in the accurate determination unit interval through breathing really from the volume of respiratory tract exhaust microorganism.
Description
Technical Field
The utility model relates to a poultry animal doctor technical field, concretely relates to pig respiratory track microorganism aerosol quantitative collection system.
Background
The existing microorganism sampler can collect microorganisms in ambient air, and the existing sampler can be matched with a quantitative detection technology to calculate the quantity of pathogenic microorganisms contained in unit air. However, the distance between the gas outlet and the gas inlet is very short, so that the collected gas cannot be repeatedly collected by equipment through the gas inlet after being discharged from the gas outlet in the collecting process, invalid circulation is caused, and the final result is inaccurate. On the other hand, the current microorganism sampler can collect pathogens in the air in local environment, but cannot accurately obtain the amount of microorganisms discharged by an animal at a specific time, and is greatly influenced by various parameters during sample collection, such as ventilation, humidity, temperature, breeding density, weight and the like.
In animal experiments such as animal models, vaccine evaluation and drug evaluation, pathogen content in samples such as nasal swabs, saliva and blood is usually adopted to indirectly measure the detoxification rule of animals under certain action conditions, so as to judge the evaluation standard of the animal models, the evaluation of vaccine immune efficiency and the evaluation of drug efficacy. However, the detection results of these samples cannot directly reflect the amount of pathogenic microorganisms actually discharged into the environment by animals, and only indirectly reflect the general rule of pathogen discharge. Some samplers can be sleeved on the pig nose for collection, but the device can collect microorganisms exhaled from the respiratory tract, collect microorganisms in the environment, interfere with the microorganisms and cannot reflect the amount of the microorganisms really exhausted from the respiratory tract of the animal.
Disclosure of Invention
In order to solve the problem, the utility model provides a pig respiratory track microorganism aerosol quantitative collection system, this collection system, simple structure can avoid the interference of original microorganism in the air, also can avoid the gas of exhalation to be collected the interference that causes after getting into the respiratory track once more in the collection process, consequently can gather live pig single breathing the volume of the microorganism or the volume of breathing really following respiratory track exhaust microorganism in the unit interval.
The purpose of the utility model is realized by adopting the following technical scheme.
A pig respiratory tract microorganism aerosol quantitative acquisition device comprises a pig mouth binding sleeve, an air inlet unit and an air outlet unit; the air inlet unit comprises an air filter, an air compressor and a valve which are sequentially connected through an air inlet pipe; the air outlet unit comprises a negative pressure air extractor, a trapping bottle and a valve which are sequentially connected through an air outlet pipe; the tail ends of the air outlet pipe and the air inlet pipe are respectively inserted into the positions of the pig mouth binding sleeve corresponding to the pig nostrils.
In the preferred technical scheme, a gas pressure gauge is arranged between a valve of the gas inlet unit and the pigsty tightening sleeve; and a gas flowmeter is arranged between the valve of the gas outlet unit and the trapping bottle.
In the preferred technical scheme, the pig mouth tightening sleeve is provided with vent holes corresponding to two nostrils of a pig respectively, and the tail ends of the air inlet pipe and the air outlet pipe penetrate out of one vent hole respectively.
In a preferred technical scheme, the quantitative collecting device is further provided with a microcomputer controller, the valve is an electromagnetic valve, and the microcomputer controller is respectively connected with an air compressor, an electromagnetic valve and a gas pressure gauge of the air inlet unit; and the microcomputer controller is also respectively connected with the negative pressure air extractor, the electromagnetic valve and the gas flowmeter of the air outlet unit.
In the utility model, the microcomputer controller can automatically record the cycle times; each circulation comprises one pumping and one pumping; pumping gas: the microcomputer controller sends a closing instruction to a negative pressure air extractor and a valve of the air outlet unit, and sends an opening instruction to an air compressor and a valve of the air inlet unit, the air compressor pumps air to the respiratory tract and the lung of the pig through an air inlet pipe, and the gas pressure gauge measures the air pressure in the end, close to the lung, of the air inlet pipe and transmits the result to the microcomputer controller; air extraction: when the gas pressure in the near-lung end of the air inlet pipe reaches a preset maximum value of the pressure intensity of the air inlet pipe, the microcomputer controller sends a closing instruction to the electromagnetic valve of the air inlet unit and the air compressor and sends an opening instruction to the electromagnetic valve of the air outlet unit and the negative pressure air pump, the negative pressure air pump pumps out the gas in the respiratory tract and the lung of the pig through the air outlet pipe, the collecting bottle collects the pumped gas, the gas pressure gauge measures the gas pressure in the near-lung end of the air inlet pipe and transmits the result to the microcomputer controller, and when the gas pressure in the near-lung end of the air inlet pipe reaches a preset minimum value of the pressure intensity of the; pumping gas and pumping air for several times.
In the preferable technical scheme, the preset maximum value of the pressure intensity of the air inlet pipe is 1.1-1.25 standard atmospheric pressures, and the preset minimum value of the pressure intensity of the air inlet pipe is 0.75-0.9 standard atmospheric pressures.
The utility model also provides an adopt the device ration gathers pig respiratory tract microorganism aerosol's method, including following step:
(1) tightening the pig mouth and sleeving the pig nose so that the tested pig can not breathe by mouth, and respectively inserting the tail ends of the air inlet pipe and the air outlet pipe into the left nostril and the right nostril;
(2) pumping gas: closing a negative pressure air extractor and a valve of the air outlet unit, opening an air compressor and a valve of the air inlet unit, and pumping air to the respiratory tract and the lung of the pig by the air compressor through an air inlet pipe; air extraction: after the air compressor of the air inlet unit works for 3-5 seconds, the air compressor and the valve of the air inlet unit are closed, the valve of the air outlet unit and a negative pressure air pump are opened, the negative pressure air pump pumps out air in the respiratory tract and the lung of the pig through an air outlet pipe, a trapping bottle collects the air pumped out from the respiratory tract of the pig, and the working time of the negative pressure air pump is 3-5 seconds;
(3) and (3) repeating the step (2) for a plurality of times, and collecting the collected liquid in the trapping bottle to obtain the porcine respiratory tract microorganism collecting liquid.
The utility model discloses in, adopt gas pressure gauge to detect the interior gas pressure of inlet tube, adopt gas flowmeter to detect the gaseous volume of collecting, the valve of the unit of admitting air and the unit of giving vent to anger is the solenoid valve.
In the utility model, the quantitative collecting device carries out a plurality of cycles, each cycle comprises one pumping and one pumping; the microcomputer controller can automatically record the cycle times; pumping gas: the microcomputer controller sends a closing instruction to a negative pressure air extractor and a valve of the air outlet unit, and sends an opening instruction to an air compressor and a valve of the air inlet unit, the air compressor pumps air to the respiratory tract and the lung of the pig through an air inlet pipe, and the gas pressure gauge measures the air pressure in the end, close to the lung, of the air inlet pipe and transmits the result to the microcomputer controller; air extraction: when the gas pressure in the near-lung end of the air inlet pipe reaches a preset maximum value of the pressure intensity of the air inlet pipe, the microcomputer controller sends a closing instruction to the electromagnetic valve of the air inlet unit and the air compressor and sends an opening instruction to the electromagnetic valve of the air outlet unit and the negative pressure air pump, the negative pressure air pump pumps out the gas in the respiratory tract and the lung of the pig through the air outlet pipe, the collecting bottle collects the pumped gas, the gas pressure gauge measures the gas pressure in the near-lung end of the air inlet pipe and transmits the result to the microcomputer controller, and when the gas pressure in the near-lung end of the air inlet pipe reaches a preset minimum value of the pressure intensity of the; pumping gas and pumping air for several times.
In the utility model, the maximum preset value of the pressure intensity of the air inlet pipe is 1.1-1.25 standard atmospheric pressures, and the minimum preset value of the pressure intensity of the air inlet pipe is 0.75-0.9 standard atmospheric pressures; the power of the air compressor and the negative pressure air extractor is 150w-300 w.
The utility model discloses pig respiratory track microorganism aerosol quantitative acquisition device, moreover, the steam generator is simple in structure, breathe with the mouth through having sealed the animal under test, make it breathe in and can only follow the pipeline entering of a nostril, exhale the pipeline exhalation that can only insert another nostril, air cleaner has been set up in the unit of admitting air, can filter air original microorganism, the gas that can avoid the exhalation simultaneously is collected the interference that causes after getting into the respiratory track once more in the collection process, consequently can gather live pig single volume of breathing the microorganism or the volume of breathing really following respiratory track exhaust microorganism in the unit interval. In the establishment of attacking the poison model, the evaluation of vaccine immunity efficiency and the medicine curative effect experimentation, can pass through the utility model discloses pig respiratory track microorganism aerosol quantitative collection device survey the rule that the animal tested discharged specific microorganism during different experiments, draw its toxin expelling (fungus) curve to this is index evaluation animal model, the immune efficiency of vaccine and the curative effect of medicine, and is more direct, objective.
Drawings
FIG. 1 is a schematic view showing the structure of the collecting apparatus in example 1, wherein 1-air inlet unit, 2-air outlet unit, 3-pig's beak binding set, 11-air filter, 12-air compressor, 13-valve, 15-air inlet pipe, 21-negative pressure air pump, 22-trap bottle, 24-valve, and 25-air outlet pipe.
FIG. 2 is a simplified structure of the present invention
The device comprises a gas inlet unit 1, a gas outlet unit 2, a pig mouth binding sleeve 3, a microcomputer controller 4, an air filter 11, an air compressor 12, an electromagnetic valve 13, a gas pressure gauge 14, a gas inlet pipe 15, a negative pressure air extractor 21, a trapping bottle 22, a gas flowmeter 23, an electromagnetic valve 24 and a gas outlet pipe 25.
Detailed Description
Example 1
The structure of the present invention will be described with reference to fig. 1.
A pig respiratory tract microorganism aerosol quantitative acquisition device comprises a pig mouth binding sleeve 3, an air inlet unit 1 and an air outlet unit 2; the air inlet unit comprises an air filter 11, an air compressor 12 and a valve 13 which are connected in sequence through an air inlet pipe 15; the air outlet unit 2 comprises a negative pressure air pump 21, a collecting bottle 22 and a valve 24 which are connected in sequence through an air outlet pipe 25; the tail ends of the air outlet pipe and the air inlet pipe are respectively inserted into the positions of the pig mouth binding sleeve corresponding to the pig nostrils. The pig mouth tightening sleeve is provided with a vent hole corresponding to the two nostrils of the pig respectively, and the tail ends of the air inlet pipe and the air outlet pipe of the quantitative collecting device penetrate out of the vent holes respectively.
The pigsty tightening sleeve comprises a cup-shaped main body and two nylon belts, the cup-shaped main body is used for being sleeved on the outer side of a pig nose, the two nylon belts are arranged at the opening of the cup-shaped main body, each nylon belt is provided with a magic tape, and the cup-shaped main body is matched with the pig nose. When the novel pig nose cap is used, the pig nose is sleeved into the cup-shaped main body, the two nylon belts are adhered through the magic tapes, and the novel pig nose cap is sleeved on the pig neck to prevent the pig from breathing with the mouth. The cup-shaped main body material is high foaming silicon rubber, the appearance of the pig mouth is attached, and the foaming silicon rubber adopts a closed bubble process, so that the pig mouth has the characteristic of high elasticity and air impermeability. Because the silica gel ware has the characteristics of difficult adhesion dirt and high temperature resistance, also be convenient for wash and high temperature disinfection operation.
The utility model discloses collection system's application method: the pig nose is sleeved with the pig mouth binding sleeve 3, the pig nose is fixed, the breathing of the oral cavity is forbidden, the tail ends of the air inlet pipe 15 and the air outlet pipe 25 are respectively plugged in the left nostril and the right nostril, so that the air flow can only enter the pig respiratory tract through the air inlet pipe, and the air flow in the pig respiratory tract can only be discharged from the air outlet pipe. Pumping gas: and opening an air compressor and a valve of the air inlet unit, wherein the negative pressure air extractor and the valve of the air outlet unit are in a closed state, and the air compressor pumps air to the respiratory tract and the lung of the pig through an air inlet pipe. Air extraction: and after the air compressor works for 3-5 seconds, the air compressor and the valve of the air inlet unit are closed, the valve of the air outlet unit and the negative pressure air pump are opened, the negative pressure air pump starts to pump air, so that the air in the respiratory tract and the lung reaches the trapping bottle through the air flow meter, and the working time of the negative pressure air pump is 3-5 seconds. The power of the air compressor and the negative pressure air extractor is 250 w. And then repeating the pumping and exhausting steps in sequence.
By adopting the method, the collection frequency can be controlled to be 6-10 times/min, and the microbial biomass exhaled for a certain time is collected circularly to measure the risk factors of the collected object in the sampling stage. Adopt the utility model discloses the device is collected pig respiratory track microorganism aerosol after the certain time, takes off the collection liquid in the entrapment bottle, and the sediment is got in the centrifugation, carries out the fluorescence quantitative PCR of various pathogenies, carries out absolute quantitative determination to the pathogen. The amounts of various microorganisms excreted from the respiratory tract and lungs by exhalation per breath (collection frequency 6-10/min) or per unit time were calculated from the test results.
Example 2
The structure of the present invention will be described with reference to fig. 2.
A pig respiratory tract microorganism aerosol quantitative acquisition device comprises a pig mouth binding sleeve 3, an air inlet unit 1 and an air outlet unit 2; the air inlet unit 1 comprises an air filter 11, an air compressor 12 and a valve 13 which are connected in sequence through an air inlet pipe 15; the air outlet unit 2 comprises a negative pressure air pump 21, a collecting bottle 22 and a valve 23 which are connected in sequence through an air outlet pipe 25; the tail ends of the air outlet pipe 25 and the air inlet pipe 15 are respectively inserted into the positions of the pig nose binding sleeve 3 corresponding to the nostrils of the pig. The pig mouth tightening sleeve is provided with a vent hole corresponding to the two nostrils of the pig respectively, and the tail ends of the air inlet pipe and the air outlet pipe of the quantitative collecting device penetrate out of the vent holes respectively.
The air filter is preferably a high efficiency air filter which prevents microorganisms in the air from entering the porcine respiratory tract.
The pig mouth binding sleeve 3 is a detachable device, and can be installed in different sizes according to the size of the pig nostrils, so that the nostrils are prevented from leaking.
The pigsty tightening sleeve comprises a cup-shaped main body and two nylon belts, the cup-shaped main body is used for being sleeved on the outer side of a pig nose, the two nylon belts are arranged at the opening of the cup-shaped main body, each nylon belt is provided with a magic tape, and the cup-shaped main body is matched with the pig nose. When the novel pig nose cap is used, the pig nose is sleeved into the cup-shaped main body, the two nylon belts are adhered through the magic tapes, and the novel pig nose cap is sleeved on the pig neck to prevent the pig from breathing with the mouth. The cup-shaped main body material is high foaming silicon rubber, the appearance of the pig mouth is attached, and the foaming silicon rubber adopts a closed bubble process, so that the pig mouth has the characteristic of high elasticity and air impermeability. Because the silica gel ware has the characteristics of difficult adhesion dirt and high temperature resistance, also be convenient for wash and high temperature disinfection operation.
And a gas pressure gauge 14 is arranged between the valve of the gas inlet unit and the pig mouth binding sleeve 3. A gas flowmeter 23 is arranged between the valve 24 of the gas outlet unit and the trapping bottle.
The utility model discloses in adopt gas pressure gauge to detect the nearly lung of intake pipe and hold interior gas pressure, adopt gas flowmeter to detect the gaseous volume of collecting, the valve is the solenoid valve.
The quantitative collecting device of the utility model is also provided with a microcomputer controller 4 which is respectively connected with an air compressor 12, a solenoid valve 13 and a gas pressure gauge 14 of the air inlet unit; the microcomputer controller is also connected with the negative pressure air pump 21, the electromagnetic valve 24 and the gas flowmeter 23 of the air outlet unit respectively.
The microcomputer controller can automatically record the circulation times, and each circulation comprises one air pumping and one air pumping. Pumping gas: the microcomputer controller sends a closing instruction to a negative pressure air extractor and a valve of the air outlet unit, and sends an opening instruction to an air compressor and a valve of the air inlet unit, the air compressor pumps air to the respiratory tract and the lung of the pig through an air inlet pipe, and the gas pressure gauge measures the air pressure in the end, close to the lung, of the air inlet pipe and transmits the result to the microcomputer controller. Air extraction: when the gas pressure in the near-lung end of the air inlet pipe reaches a preset maximum value of the pressure intensity of the air inlet pipe, the microcomputer controller sends a closing instruction to the electromagnetic valve of the air inlet unit and the air compressor and sends an opening instruction to the electromagnetic valve of the air outlet unit and the negative pressure air pump, the negative pressure air pump pumps out the gas in the respiratory tract and the lung of the pig through the air outlet pipe, the collecting bottle collects the pumped gas, the gas pressure gauge measures the gas pressure in the near-lung end of the air inlet pipe and transmits the result to the microcomputer controller, and when the gas pressure in the near-lung end of the air inlet pipe reaches a preset minimum value of the pressure intensity of; pumping gas and pumping air for several times.
The maximum preset value of the pressure intensity of the air inlet pipe is 1.25 standard atmospheric pressures, and the minimum preset value of the pressure intensity of the air inlet pipe is 0.75 standard atmospheric pressure.
The utility model discloses a use method: the pig nose is sleeved with the pig mouth binding sleeve 3, the pig nose is fixed, the breathing of the oral cavity is forbidden, the tail ends of the air inlet pipe 15 and the air outlet pipe 25 are respectively plugged in the left nostril and the right nostril, so that the air flow can only enter the pig respiratory tract through the air inlet pipe, and the air flow in the pig respiratory tract can only be discharged from the air outlet pipe. The power supply is started, the quantitative collecting device carries out a plurality of cycles, each cycle comprises one time of pumping and one time of pumping, and the microcomputer controller can automatically record the cycle times. Pumping gas: the microcomputer controller sends a closing instruction to a negative pressure air extractor and a valve of the air outlet unit, sends an opening instruction to an air compressor 12 and an electromagnetic valve 13 of the air inlet unit, the air compressor 12 pumps air to the respiratory tract and the lung of the pig through an air inlet pipe, and the air pressure gauge measures the air pressure in the end, close to the lung, of the air inlet pipe and transmits the result to the microcomputer controller. Air extraction: when the gas pressure in the near-lung end of the air inlet pipe reaches a preset maximum value of the pressure intensity of the air inlet pipe, the microcomputer controller sends a closing instruction to the electromagnetic valve 13 and the air compressor 12 of the air inlet unit and sends an opening instruction to the electromagnetic valve 24 and the negative pressure air pump 21 of the air outlet unit, the negative pressure air pump pumps out the gas in the respiratory tract and the lung of the pig through the air outlet pipe, the collecting bottle collects the pumped gas, the gas pressure gauge measures the gas pressure in the near-lung end of the air inlet pipe and transmits the result to the microcomputer controller, and when the gas pressure in the near-lung end of the air inlet pipe reaches a preset minimum value of the pressure intensity of; the method is used for pumping air and pumping air for a plurality of times in sequence, and the quantitative collection of the microbial aerosol in a certain time can be completed. The maximum preset value of the pressure intensity of the air inlet pipe is 1.25 standard atmospheric pressures, and the minimum preset value of the pressure intensity of the air inlet pipe is 0.75 standard atmospheric pressure. The power of the air compressor and the negative pressure air extractor during working is 220w, the collection frequency is 8 cycles/min, and the microbial biomass exhaled in a certain time is collected in a circulating mode to measure risk factors of the collected object in the sampling stage. Adopt the utility model discloses the device is collected pig respiratory track microorganism aerosol after the certain time, takes off the collection liquid in the entrapment bottle, and the sediment is got in the centrifugation, carries out the fluorescence quantitative PCR of various pathogenies, carries out absolute quantitative determination to the pathogen. And calculating the amount of various microorganisms discharged in each breath according to the detection result.
Claims (6)
1. A pig respiratory tract microorganism aerosol quantitative acquisition device is characterized by comprising a pig mouth binding sleeve, an air inlet unit and an air outlet unit; the air inlet unit comprises an air filter, an air compressor and a valve which are sequentially connected through an air inlet pipe; the air outlet unit comprises a negative pressure air extractor, a trapping bottle and a valve which are sequentially connected through an air outlet pipe; the tail ends of the air outlet pipe and the air inlet pipe are respectively inserted into the positions of the pig mouth binding sleeve corresponding to the pig nostrils.
2. The quantitative collecting device for the pig respiratory tract microorganism aerosol according to claim 1, characterized in that a gas pressure gauge is arranged between a valve of the air inlet unit and the pig mouth tightening sleeve; and a gas flowmeter is arranged between the valve of the gas outlet unit and the trapping bottle.
3. The device for quantitatively collecting the porcine respiratory microorganism aerosol as claimed in claim 2, wherein the pig mouth binding sleeve is provided with vent holes corresponding to two nostrils of the pig respectively, and the tail ends of the air inlet pipe and the air outlet pipe respectively penetrate out of one vent hole.
4. The pig respiratory tract microorganism aerosol quantitative acquisition device according to any one of claims 1 to 3, characterized in that the quantitative acquisition device is further provided with a microcomputer controller, the valve is an electromagnetic valve, and the microcomputer controller is respectively connected with an air compressor, the electromagnetic valve and a gas pressure gauge of the air inlet unit; and the microcomputer controller is also respectively connected with the negative pressure air extractor, the electromagnetic valve and the gas flowmeter of the air outlet unit.
5. The quantitative acquisition device for the pig respiratory tract microorganism aerosol as claimed in claim 4, wherein the microcomputer controller can automatically record the cycle number; each circulation comprises one pumping and one pumping; pumping gas: the microcomputer controller sends a closing instruction to a negative pressure air extractor and a valve of the air outlet unit, and sends an opening instruction to an air compressor and a valve of the air inlet unit, the air compressor pumps air to the respiratory tract and the lung of the pig through an air inlet pipe, and the gas pressure gauge measures the air pressure in the end, close to the lung, of the air inlet pipe and transmits the result to the microcomputer controller; air extraction: when the gas pressure in the near-lung end of the air inlet pipe reaches a preset maximum value of the pressure intensity of the air inlet pipe, the microcomputer controller sends a closing instruction to the electromagnetic valve of the air inlet unit and the air compressor and sends an opening instruction to the electromagnetic valve of the air outlet unit and the negative pressure air pump, the negative pressure air pump pumps out the gas in the respiratory tract and the lung of the pig through the air outlet pipe, the collecting bottle collects the pumped gas, the gas pressure gauge measures the gas pressure in the near-lung end of the air inlet pipe and transmits the result to the microcomputer controller, and when the gas pressure in the near-lung end of the air inlet pipe reaches a preset minimum value of the pressure intensity of the; pumping gas and pumping air for several times.
6. The device for quantitatively collecting the porcine respiratory microorganism aerosol as claimed in claim 5, wherein the preset maximum value of the pressure of the air inlet pipe is 1.1-1.25 standard atmospheres, and the preset minimum value of the pressure of the air inlet pipe is 0.75-0.9 standard atmospheres.
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CN110567766A (en) * | 2019-10-17 | 2019-12-13 | 江苏省农业科学院 | Pig respiratory track microorganism aerosol quantitative collection system |
CN110567766B (en) * | 2019-10-17 | 2024-07-02 | 江苏省农业科学院 | Pig respiratory tract microorganism aerosol quantitative acquisition device |
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