CN209346995U - A kind of record conscious mouse respiratory movement and habituation and remove habituation device - Google Patents
A kind of record conscious mouse respiratory movement and habituation and remove habituation device Download PDFInfo
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- CN209346995U CN209346995U CN201721659639.6U CN201721659639U CN209346995U CN 209346995 U CN209346995 U CN 209346995U CN 201721659639 U CN201721659639 U CN 201721659639U CN 209346995 U CN209346995 U CN 209346995U
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- habituation
- mouse
- micro controller
- breathing
- record
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Abstract
The utility model relates to a kind of record conscious mouse respiratory movement and habituation and remove habituation device, it is characterized by: it includes for detecting mouse breathing situation and generating the breathing recording device of corresponding event signal, for monitoring mouse movement situation and generating the motion profile monitoring device of corresponding motor message, for monitor mouse to Odor stimulation or habituation with go habituation training mission and generate corresponding detection signal to device of air, for receiving event signal, motor message and the micro controller for detecting signal analysis and processing；For showing that the display of each data, the display are connected to micro controller.The utility model whole experiment process is full-automation, only needs to be switched in experiment and is put into mouse and connects connecting line；The device can accurate recording mouse habituation with go habituation during breathing and motion profile signal, thus for breathing advantageous scientific basis is provided during detected by scent.
Description
Technical field
The utility model relates to a kind of record conscious mouse respiratory movement and habituation and remove habituation device.
Background technique
The detected by scent process of humans and animals is required by the way that constantly periodically air-breathing is extraneous to obtain and update
Matter information is smelt, therefore respiratory is most important to the perception of olfactory information, this pays close attention to many smell researchers always to exhale
Inhale effect during detected by scent.A large amount of correlative studys in recent years be related to people and rat and it is various types of other
Animal, especially compared on toy such as mouse research get up relative difficulty；And the experiment of traditional record animal breath movement is more
It is measured using trachea cannula pneumography, recorder method (physiograph method) and using movement of thorax, this kind of side
Method is although simple and easy, but have damage, take time and effort, accuracy it is inadequate；And at present respiratory movement for mouse habituation with
It goes role during habituation still unclear, also there is no accurate effective method by conscious mouse respiratory movement and habit
Usedization combines with the device of habituation is removed.
Utility model content
For the above, the purpose of this utility model is to provide a kind of record conscious mouse respiratory movement and habituations
With remove habituation device, with solve the above problems at least one aspect.
In order to realize that technical solution used by the purpose of this utility model is: a kind of record conscious mouse respiratory movement and
Habituation and remove habituation device, it is characterised in that: it includes for detecting mouse breathing situation and generating corresponding event signal
Breathing recording device be used for for monitoring mouse movement situation and generating the motion profile monitoring device of corresponding motor message
Monitoring mouse to Odor stimulation or habituation with go habituation training mission and generate it is corresponding detect signal to device of air, be used for
Receive event signal, motor message and the micro controller for detecting signal analysis and processing；It is described for showing the display of each data
Display be connected to micro controller；
Breathing recording device, motion profile monitoring device and the output end to device of air respectively connected micro controller
Input terminal, by micro controller to event signal, motor message and detection signal be analyzed and processed, finally mouse is exhaled
The physiology and appearance of suction, smell and movement is monitored in real time.
Further, the breathing recording device includes breathing casing, small hose, baroceptor and amplifier；It is described
Breathing casing be made of conduit and conduit cap, conduit cap is socketed on conduit, and mouse suitable position is inserted into one end of conduit
Nasal cavity in, the other end is connected to that leakproofness is good, is suitble to the small hose of diameter.
Further, the baroceptor is caused soft intraductal atmospheric pressure in the expiration of detection mouse and breathing process
Variation and generate breathing inductive signal, described in baroceptor be located in small hose, the output end of baroceptor connects
Then amplifier.
Further, the amplifier is the breathing inductive signal amplification that will be recorded, and the input terminal of amplifier is connected to
Baroceptor, output end are connected to micro controller, so that amplified breathing inductive signal is transmitted to micro controller, micro controller pair
Inductive signal is transmitted to display and is shown after being handled.
Further, the motion profile monitoring device include for monitor and record the camera of mouse movement track and
Strut, the camera are located at the top that can monitor mouse movement track, and camera is connected to micro controller, thus by being acquired
To motor message be transmitted to micro controller, micro controller is shown to being transmitted on display after motor message processing；Described
Strut is used for fixing camera.
Further, described to include training place and multi-channel gas generation device to device of air.
Further, the training place is the place that mouse can move freely in certain spacious space.
Further, the multi-channel gas generation device is by gas pipeline, flowmeter, solenoid valve, odor bottle, air pressure
Contracting pump, relay and infrared tube composition；Described gas pipeline one end is connected to odor bottle, and the other end is connected to training place；It is described
Odor bottle inside be equipped with a certain proportion of odor liquids；The air pressure pump is connected to odor bottle, passes through air pressure pump
Give odor bottle certain air pressure, by the control of flowmeter, so that the smell in odor bottle is transported to by gas pipeline
Training place so that mouse sucking smell and realize the stimulation of smell.
Further, the solenoid valve is connected in connecting tube, and solenoid valve is connected to relay；The relay is defeated
Enter end and is connected to micro controller.
Further, the flowmeter is located on gas pipeline, and the output end of flowmeter is connected to micro controller.
Further, the infrared tube is located at the side of gas pipeline gas outlet in training place, infrared tube it is defeated
Outlet is connected to micro controller, and the infrared tube can monitor that mouse exploration goes the action signal of smelling the moment, and will
The information is transmitted to micro controller.
Further, data acquisition module is equipped in the micro controller, thus by data acquisition module to each data
It is acquired and saves.
Further, be equipped with can be with the amplifier, camera, relay, flowmeter and infrared for the micro controller
The communication control interface compatible to pipe.
The utility model has the beneficial effects that
1, whole experiment process is full-automation, only needs to be switched in experiment and is put into mouse and connects connecting line i.e.
Can, other are all automatically performed by animal and the device.
2, the device can accurate recording mouse habituation with go habituation during breathing and motion profile signal,
To provide advantageous scientific basis during detected by scent for breathing；
3, the device can accurate recording mouse habituation with go habituation during breathing and motion profile signal,
To provide advantageous scientific basis during detected by scent for breathing；It is simultaneously also that evaluation relevant nerve diseases treatment is new
The powerful of medicine；
4, the breathing recording device is easy to operate, small to the damage of mouse, convenient and easy, very big to realize mouse and instructing
Practice moveing freely for place；
5, it by monitoring the respiratory rate of mouse, on the one hand can be controlled according to the frequency of breathing to gas, on the other hand
Breathing role in odor discrimination can be studied；
6, the camera monitoring device used, can detect motion profile of mouse during experiment at any time, thus
The effect for being study movement in olfactory function provides a kind of foundation.
Detailed description of the invention
Fig. 1 is the utility model overall structure diagram.
Fig. 2 is breathing cannula structure schematic diagram figure.
Fig. 3 is gas generation apparatus structural schematic diagram.
Fig. 4 is the utility model circuit diagram.
In figure, 1- camera, 2- strut, 3- micro controller, 4- display, 5- baroceptor, 6- amplifier, 7- flue
Road, 8- gas generation apparatus, 9- infrared tube, 10- mouse, 11- training place, 12- conduit cap, 13- conduit, 14- nasal cavity,
15- odor bottle, 16- solenoid valve, 17- relay, 18- air pressure pump, 19- flowmeter.
Specific embodiment
The utility model is described in further detail in the following with reference to the drawings and specific embodiments.
Fig. 1, Fig. 2, Fig. 3, Fig. 4 schematically show a kind of record conscious mouse respiratory movement of the utility model and habit
Change and go the structure of habituation device.
As shown in Figure 1, Figure 2, Figure 3, Figure 4, a kind of record conscious mouse respiratory movement and habituation and habituation device is removed,
It is characterized by: it includes for detecting mouse breathing situation and generating the breathing recording device of corresponding event signal, for supervising
It surveys mouse movement situation and generates the motion profile monitoring device of corresponding motor message, for monitoring mouse to Odor stimulation or habit
Usedization with go habituation training mission and generate corresponding detection signal to device of air, for receiving event signal, motor message
With the micro controller of detection signal analysis and processing and display；
Breathing recording device, motion profile monitoring device and the output end to device of air respectively connected micro controller
Input terminal, by micro controller to event signal, motor message and detection signal be analyzed and processed, finally mouse is exhaled
The physiology and appearance of suction, smell and movement is monitored and is recorded in real time.
As shown in Figure 1 and Figure 2, the breathing recording device includes breathing casing, small hose, baroceptor and amplification
Device；The breathing casing is made of conduit and conduit cap, and conduit cap is socketed on conduit, and one end insertion mouse of conduit is suitable
In the nasal cavity that coincidence is set, the other end is connected to the small hose of good, the suitable diameter of leakproofness.
As shown in Figure 1 and Figure 2, the baroceptor is caused jewelry, expensive clothing and other valuables in the expiration of detection mouse and breathing process
The variation of intraductal atmospheric pressure and generate breathing inductive signal, described in baroceptor be located in small hose, baroceptor
Output end is connected to amplifier.
As shown in Figure 1 and Figure 2, the amplifier is the breathing inductive signal amplification that will be recorded, the input terminal of amplifier
Be connected to baroceptor, output end is connected to micro controller, thus amplified breathing inductive signal is transmitted to micro controller and
It is shown.
As shown in Figure 1 and Figure 2, the surgical procedure for breathing casing loading mouse nasal cavity is as follows: on the mouse nasal cavity of anesthesia
The aperture and the broken bone in cleaning hole of drill with ferrule proper diameter, one end of conduit is inserted in the hole, is around wrapped up with dental cement
Get up, after cement drying, by the other end of conduit cap insertion conduit and tightens, to prevent blocking nasal cavity；In the course of surgery,
In order to judge the accurate of catheter insertion site, therefore before insertion, a small amount of physiological saline is first dripped in the top in hole, observes water
Whether face is fluctuated therewith breathing, and illustrates that position is accurate if having fluctuation；After operation is completed, it is pre- to give mouse anti-infectives
It is anti-, it is placed in rest 3-5 days in cage；After state recovery after can recording respiration, conduit cap is unscrewed and is taken out, connect small hose,
Baroceptor and signal amplifier etc.；If breathing is not recorded for the first time, need to be rapidly injected air with 1ml syringe
Keep its unobstructed until breathing is recorded, the state of observation mouse is also needed in venting process, to prevent the excessive air of injection
It is damaged to alveolar tissue etc..
As shown in Figure 1 and Figure 2, the breathing recording device is in the nasal cavity by breathing catheter insertion mouse, and the other end connects
Then leakproofness is good, is suitble to the small hose of diameter；The variation of breathing inductive signal, Jin Ertong are monitored by baroceptor
Inductive signal amplification will be breathed by crossing signal amplifier, and amplified breathing inductive signal is transmitted to micro controller, at this time micro-control
Device is acquired to breathing inductive signal and shows corresponding data by display screen, so as to accurately realize respiratory rate with
And the record of amplitude.
As shown in Figure 1, Figure 3, described to include training place and multi-channel gas generation device to device of air.
As shown in Figure 1, Figure 3, the training place is the place that mouse can move freely in certain spacious space.
As shown in Figure 1, Figure 3, the multi-channel gas generation device is by gas pipeline, flowmeter, solenoid valve, smell
Bottle, air pressure pump, connecting tube, relay and infrared tube composition；Described gas pipeline one end is connected to odor bottle, the other end
It is connected to training place；A certain proportion of odor liquids are equipped with inside the odor bottle；The air pressure pump is connected to odor bottle,
Give odor bottle certain air pressure by air pressure pump, by the control of flowmeter, so that the smell in odor bottle be passed through
Gas pipeline is transported to training place so that mouse sucking smell and realize the stimulation of smell.
As shown in Figure 1, Figure 3, the odor bottle is equipped with multiple, and a kind of odor liquids are loaded in each odor bottle；Institute
The connecting tube stated be equipped with it is multiple, one end of connecting tube is connected to the output end of air pressure pump, and the other end is connected to a smell
Bottle.
As shown in Figure 1, Figure 3, the solenoid valve is equipped with multiple, and solenoid valve is located in connecting tube, in each connecting tube
Equipped with a solenoid valve, solenoid valve is connected to relay；The relay input terminal is connected to micro controller.
As shown in Figure 1, Figure 3, the micro controller issues control instruction to relay, so that relay controls an electromagnetism
Valve is opened, so that the gas that air pressure pump generates passes through the corresponding odor bottle of the solenoid valve, so that gas has the odor bottle
The smell of interior odor liquids, and exported by gas pipeline, mouse can receive the thorn of smell by the end of gas pipeline
Swash.
As shown in Figure 1, Figure 3, the flowmeter is located on gas pipeline, and the output end of flowmeter is connected to micro controller,
When gas flows in odor bottle, flowmeter generates flow signal and is sent to micro controller, so that micro controller passes through data acquisition module
Flow signal is acquired.
As shown in Figure 1, Figure 3, the infrared tube is located at the side of gas pipeline gas outlet in training place, infrared right
The output end of pipe is connected to micro controller, and the infrared tube can monitor that mouse exploration goes the action signal of smelling the moment
And the time of smelling, and transmit this information to micro controller.
As shown in Figure 1, Figure 3, when experiment starts, the mouse being trained will be needed to shift to an earlier date ten minutes and be placed on training place
Institute, and then mouse is allowed to adapt to environment, the switch for next adjusting flowmeter arrives the scale of very little, generates dress by multi-channel gas
It sets and gives a kind of smell, continue two minutes, to depressed beam after two minutes, multi-channel gas generation device repeats above behaviour
Make, is repeated 4 times with this；To the 5th, another smell will be given to gas system, repeats above operation.
As shown in Figure 1, Figure 3, the gas pipeline is replaceable pipeline, to avoid obscuring for smell and cause dirt
Dye.
As shown in Figure 1, Figure 3, the training place is will to need to be trained small for the movable place of mouse
Mouse is placed on trained place, and then mouse is allowed to adapt to environment.
As shown in Figure 1, the micro controller is connect with computer communication, to can check exhaling for mouse by computer
It inhales, the physiology and appearance data information of smell and movement.
As shown in Figure 1, the motion profile monitoring device includes the camera shooting for monitoring and recording mouse movement track
Head and strut, the camera are located at the top that can monitor mouse movement track, and camera is connected to micro controller, thus by institute
Collected motor message is transmitted to micro controller, and micro controller is transmitted on display after handling motor message and shows；Institute
The strut stated is used for fixing camera.
As Figure 1 and Figure 4, electronic switch, the input terminal of electronic switch are additionally provided between camera and micro controller connection
It is connected to micro controller, output end is connected to camera；During the test, micro controller issues control instruction to electronic switch, from
And the opening of electronic switch is controlled, so that camera starts to image, the information for monitoring toy is transmitted to micro controller and is saved simultaneously
Corresponding computer is transmitted this information to by micro controller, so as to check the fortune of toy during the experiment by computer
Dynamic state.
As shown in Figure 1, Figure 2, Figure 3, Figure 4, the standard value of mouse breathing frequency, mouse breathing are equipped in the micro controller
Respiratory rate in metastable situation in a certain range (general 2-5Hz), when baroceptor detects mouse
When respiratory rate is below or above standard value, micro controller generates control instruction and is sent to relay, so that relay connection
Solenoid valve is obtained electric and is attracted, so that the air in odor bottle is flowed into training place by gas pipeline, supplies breathing institute to mouse
The stimulation of the air and smell that need.
As shown in Figure 1, Figure 2, Figure 3, Figure 4, the information that the micro controller is transmitted by flowmeter is to gas flow
Data are acquired, while can also be triggered by the variation for the respiratory rate being recorded to device of air；Mouse breathing is opposite
In the case where stabilization, so that triggering comes to give mouse certain density Odor stimulation to device of air.
As shown in Figure 1, Figure 2, Figure 3, Figure 4, the present apparatus is additionally provided with control switch, and control switch is connected to power supply, another
End is separately connected each electronic device, so that when needing to start test control switch opening can be provided to electric energy to the present apparatus,
Consequently facilitating the work of each electronic device.
As shown in Figure 1, Figure 2, Figure 3, Figure 4, OPA234 model amplifier or AD620BR model can be selected in the amplifier
Amplifier etc..
As shown in Figure 1, Figure 2, Figure 3, Figure 4, the micro controller using can analyze, control, the micro controller of timing and storage
(such as: 424-CHIPKIT-UC32 micro controller, EP1K100QC208-3 micro controller or 410-301 micro controller).
Above-described is only some embodiments of the utility model.For those of ordinary skill in the art,
Without departing from the concept of the present invention, at least one modification and improvement can also be made, these belong to this
The protection scope of utility model.
Claims (10)
1. a kind of record conscious mouse respiratory movement and habituation and removing habituation device, it is characterised in that: it includes for examining
It surveys mouse breathing situation and generates the breathing recording device of corresponding event signal, for monitoring mouse movement situation and generating correspondence
The motion profile monitoring device of motor message, for monitoring mouse to Odor stimulation or habituation and going habituation training mission simultaneously
The corresponding detection signal of generation gives device of air, for receiving event signal, motor message and the micro-control for detecting signal analysis and processing
Device；For showing that the display of each data, the display are connected to micro controller；
Breathing recording device, motion profile monitoring device and the output end to device of air respectively connected the defeated of micro controller
Enter end, event signal, motor message and detection signal is analyzed and processed by micro controller, finally to the breathing of mouse, gas
Taste and the physiology and appearance of movement are monitored in real time.
2. a kind of record conscious mouse respiratory movement according to claim 1 and habituation and habituation device is removed, it is special
Sign is: the breathing recording device includes breathing casing, small hose, baroceptor and amplifier；The breathing set
Pipe is made of conduit and conduit cap, and conduit cap is socketed on conduit, in the nasal cavity of one end insertion mouse suitable position of conduit,
The other end is connected to the small hose of good, the suitable diameter of leakproofness.
3. a kind of record conscious mouse respiratory movement according to claim 2 and habituation and habituation device is removed, it is special
Sign is: the baroceptor be detect mouse exhale and breathing process in caused soft intraductal atmospheric pressure variation and
Generate breathing inductive signal, described in baroceptor be located in small hose, the output end of baroceptor is connected to amplify
Device.
4. a kind of record conscious mouse respiratory movement according to claim 2 and habituation and habituation device is removed, it is special
Sign is: the amplifier is the breathing inductive signal amplification that will be recorded, and the input terminal of amplifier is connected to air pressure sensing
Device, output end are connected to micro controller, so that amplified breathing inductive signal is transmitted to micro controller, micro controller is to inductive signal
Display is transmitted to after being handled and is shown.
5. a kind of record conscious mouse respiratory movement according to claim 1 and habituation and habituation device is removed, it is special
Sign is: the motion profile monitoring device includes the camera and strut for monitoring and recording mouse movement track, institute
The camera stated is located at the top that can monitor mouse movement track, and camera is connected to micro controller, thus by the collected fortune of institute
Dynamic signal is transmitted to micro controller, and micro controller is transmitted on display after handling motor message and shows；The strut is used
In fixing camera.
6. a kind of record conscious mouse respiratory movement according to claim 1 and habituation and habituation device is removed, it is special
Sign is: described to include training place and multi-channel gas generation device to device of air；The training place is that mouse exists
The place that can be move freely in certain spacious space.
7. a kind of record conscious mouse respiratory movement according to claim 6 and habituation and habituation device is removed, it is special
Sign is: the multi-channel gas generation device by gas pipeline, flowmeter, solenoid valve, odor bottle, air pressure pump, after
Electric appliance and infrared tube composition；Described gas pipeline one end is connected to odor bottle, and the other end is connected to training place；The odor bottle
The inside is equipped with a certain proportion of odor liquids；The air pressure pump is connected to odor bottle, gives smell by air pressure pump
The certain air pressure of bottle, so that the smell in odor bottle is transported to training place by gas pipeline, is made by the control of flowmeter
Obtain the stimulation that mouse sucks smell and realizes smell.
8. a kind of record conscious mouse respiratory movement according to claim 7 and habituation and habituation device is removed, it is special
Sign is: the solenoid valve is connected in connecting tube, and solenoid valve is connected to relay；The relay input terminal connection
Micro controller；The flowmeter is located on gas pipeline, and the output end of flowmeter is connected to micro controller.
9. a kind of record conscious mouse respiratory movement according to claim 7 and habituation and habituation device is removed, it is special
Sign is: the infrared tube is located at the side of gas pipeline gas outlet in training place, the output end connection of infrared tube
Micro controller, the infrared tube can monitor that mouse exploration goes the action signal of smelling the moment, and the information is passed
It is sent to micro controller.
10. a kind of record conscious mouse respiratory movement according to claim 1 and habituation and habituation device is removed, it is special
Sign is: data acquisition module is equipped in the micro controller, to be acquired by data acquisition module to each data
And it saves；The micro controller is equipped with can be compatible with the amplifier, camera, relay, flowmeter and infrared tube
Communication control interface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721659639.6U CN209346995U (en) | 2017-12-01 | 2017-12-01 | A kind of record conscious mouse respiratory movement and habituation and remove habituation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721659639.6U CN209346995U (en) | 2017-12-01 | 2017-12-01 | A kind of record conscious mouse respiratory movement and habituation and remove habituation device |
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| Publication Number | Publication Date |
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| CN209346995U true CN209346995U (en) | 2019-09-06 |
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| CN201721659639.6U Expired - Fee Related CN209346995U (en) | 2017-12-01 | 2017-12-01 | A kind of record conscious mouse respiratory movement and habituation and remove habituation device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107874743A (en) * | 2017-12-01 | 2018-04-06 | 徐州医科大学 | One kind record conscious mouse respiratory movement and habituation are with removing habituation device |
-
2017
- 2017-12-01 CN CN201721659639.6U patent/CN209346995U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107874743A (en) * | 2017-12-01 | 2018-04-06 | 徐州医科大学 | One kind record conscious mouse respiratory movement and habituation are with removing habituation device |
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Granted publication date: 20190906 Termination date: 20201201 |