CN104458348B - Method and device for collecting PAEs in gas exhaled by human body - Google Patents
Method and device for collecting PAEs in gas exhaled by human body Download PDFInfo
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- CN104458348B CN104458348B CN201410694843.6A CN201410694843A CN104458348B CN 104458348 B CN104458348 B CN 104458348B CN 201410694843 A CN201410694843 A CN 201410694843A CN 104458348 B CN104458348 B CN 104458348B
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- absorbent
- human body
- paes
- gas
- detergent
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Abstract
The invention discloses a method and a device for collecting PAEs in gas exhaled by a human body. The method comprises the following steps: step 1, carrying out pretreatment on an absorbent by using a Soxhlet extraction method; step 2, after the completion of the pretreatment, taking out the absorbent in a gauze bag and carrying out rotary evaporation under the negative atmospheric pressure till the absorbent is dry, wherein the oil bath temperature in the rotary evaporation is controlled within 44-46 DEG C; then putting the absorbent in an airtight glass container for later use; step 3, collecting the gas exhaled by the human body into an absorber filled with the absorbent, wherein the absorbent can absorb the PAEs in the gas exhaled by the human body and other organic pollutants. According to the method and the device, the collection of specific volatile pollutants, semi-volatile organic pollutants and the like by the human body through a respiratory pathway can be simply and feasibly realized, so that a basis is provided for further researching the absorption efficiency of a human body respiratory tract, evaluating the effect of specific pollutants in indoor air on human body health and the like.
Description
Technical field
The invention belongs to the chemical environment pollution of Environmental Chemistry and health field, more particularly, to a kind of collection human body expired gass
The method of paes and its use device in body.
Background technology
For a long time, the impact to health for the environmental contaminants of low concentration is always the difficulty of environmental toxicology research field
Topic, and first its Research Challenges is to be difficult to determine the absorption efficiency to pollutant for the human body.At present, it is similar to invention or document is related to
Characteristics of contaminated respiratory droplets gas area research, be concentrated mainly in detection method, such as: simultaneously detection characteristics of contaminated respiratory droplets gas in ebcs and
The integrated analysis device (application publication number: cn103168233a) of vocs, and for example: in a kind of detection characteristics of contaminated respiratory droplets gas, no contains
The method (application publication number: cn102507720a) of amount.So far also there is no an invention, be directed exclusively to characteristics of contaminated respiratory droplets collection and confinement of gases
Device.The commercially available instrument with regard to collecting human body exhaled gas is not had on market；And it is directed to the research of the above-mentioned type, its experiment
During if desired collect human body exhaled gas, or complex operation, process take time and effort, or instrument price is expensive.
At present, on market, the higher home ventilator of degree of recognition has the double level respirator of Rui Simai s9 vpap st, flies
Double level respirator of the big health bipap s/t in sharp Pu etc., its price all between 10,000 yuan～20,000 yuans, hospital intensive care
The invasive respirator of the uses such as ward, price is then higher.The present invention is different from above-mentioned medical auxiliary equipment human body respiration device, main
The research to aspects such as Health Impacts for the pollutant of in the air to be used for.
Content of the invention
The purpose of the present invention is for the deficiencies in the prior art, provides a kind of method collecting paes in human body exhaled gas
And its use device.
The technical solution adopted for the present invention to solve the technical problems is as follows:
A kind of method collecting paes in human body exhaled gas, comprises the steps:
Step 1, with soxhlet extraction, pre-treatment is carried out to absorbent；
1-1. loads absorbent in handss seam gauze bag, then handss seam gauze bag is put in apparatus,Soxhlet'ses, surname extraction
Terminate flask under device, pour detergent dichloromethane into apparatus,Soxhlet'ses, until the amount energy submergence gauze of detergent dichloromethane
Till bag；The bath temperature of flask is controlled at 60 DEG C～65 DEG C, carries out washing for the first time, wash time is 46h～50h；
After the completion of 1-2. washs for the first time, it is cooled to room temperature, abandons the detergent dichloromethane in most flask, then to rope
Detergent methanol is poured again into, till the amount energy submergence gauze bag of detergent methanol, now by flask in family name's extractor
Bath temperature controls at 80 DEG C～83 DEG C, carries out second washing, and wash time is 46h～50h；
After the completion of step 2, twice washing, the absorbent in gauze bag is taken out, under negative atmospheric pressure, revolving, to being dried, is revolved
The oil bath temperature steaming controls at 44 DEG C～46 DEG C；Then absorbent is placed on closed glass containers for future use；
Step 3, by the collection and confinement of gases of characteristics of contaminated respiratory droplets in the absorber equipped with absorbent, absorbent can absorb human body and exhale
Organic pollution in the gas going out.
Described absorbent is the organic resin of polyethylene kind, such as: the absorbent for paes is xad-2 resin, for
The absorbent of voc is tenax-ta resin.
It is pure that described detergent dichloromethane and methanol are analysis.
The device that a kind of method collecting paes in human body exhaled gas is used, including acquisition port, safety and filtration dress
Put and absorber；Acquisition port is patch skin-type full bag mouth dew nose formula plastic mask, and both sides are coupled with rubber band, are fixed on two ears, examination
Holding snuffing gas during testing, exhaled with mouth；Acquisition port is connected by rubber tube with the air inlet of filtering device with safety, peace
Entirely it is connected by rubber tube with the air inlet of absorber with the gas outlet of filtering device；Absorber upper end is provided with gas outlet, with
The external world is connected.
Described safety is special u-shaped glass sampling tube with filtering device, is inside provided with quartz particles interlayer.
It is placed with the absorbent through pre-treatment in described absorber.
The present invention has the beneficial effect that:
The present invention is different from the medical auxiliary equipment such as commercially available respiratory organ on market, can realize human body to simple possible
By the collection to the pollutant in special air for the respiratory pathwayss, realize sucking pollutant levels to human body and exhalation pollutant are dense
The sampling respectively of degree, thus the pollutant for research specific pollutants respiratory tract absorption efficiency further, the indoor in the air of assessment
Place mat is made in the research such as impact to health.
Apparatus of the present invention can be realized human body being sucked with the sampling respectively of pollutant levels and exhalation pollutant levels, after being
Continuous mensure is realized providing feasible approach with correlational study.
Brief description
Fig. 1 is apparatus of the present invention schematic diagram.
In figure: acquisition port 1, air inlet 2, safety and filtering device 3, absorber 4, gas outlet 5.
Specific embodiment
A kind of method collecting paes in human body exhaled gas, specifically includes following steps:
Step 1, with soxhlet extraction, pre-treatment is carried out to absorbent.
1-1. loads absorbent in handss seam gauze bag, then handss seam gauze bag is put in apparatus,Soxhlet'ses, surname extraction
Terminate flask under device, pour detergent dichloromethane into apparatus,Soxhlet'ses, until the amount submergence gauze bag of detergent dichloromethane
Till；The bath temperature of flask is controlled at 60～65 DEG C, carries out washing for the first time, wash time is 46h～50h；
After the completion of 1-2. washs for the first time, it is cooled to room temperature, abandons the detergent dichloromethane in most flask, then to rope
Detergent methanol is poured again into, till the amount submergence gauze bag of detergent methanol, now by the water of flask in family name's extractor
Bath temperature controls at 80～83 DEG C, carries out second washing, and wash time is 46h～50h；
Described absorbent is the organic resin of polyethylene kind, such as: the absorbent for paes is xad-2 resin, for
The absorbent of voc is tenax-ta resin；
It is pure that described detergent dichloromethane and methanol are analysis.
After the completion of step 2, twice washing, absorbent in gauze bag is taken out, under negative atmospheric pressure, uses rotary evaporator re-
To being dried, the oil bath temperature of revolving controls at 44 DEG C～46 DEG C 52aa revolving；Then absorbent is placed on closed glass container
In standby.
Described negative atmospheric pressure is no strict with, and uses common shb-The evacuation such as multiplex vavuum pump of circulating water type.
Step 3, by the collection and confinement of gases of characteristics of contaminated respiratory droplets in the absorber of the organic resin absorbent equipped with polyethylene kind, inhale
Receive agent and can absorb the organic pollutions such as the paes in the gas of characteristics of contaminated respiratory droplets.
The device that a kind of method collecting paes in human body exhaled gas is used, including acquisition port 1, safety and filtration dress
Put 3 and absorber 4；Acquisition port 1 is patch skin-type full bag mouth dew nose formula plastic mask, and both sides are coupled with rubber band, are fixed on two ears,
Holding snuffing gas in process of the test, exhaled with mouth；Acquisition port 1 is connected by rubber tube with the air inlet 2 of safety and filtering device 3
Connect, safety is connected by rubber tube with the air inlet of absorber 4 with the gas outlet of filtering device 3；Absorber 4 upper end is provided with out
QI KOU 5, gas outlet 5 is connected with ambient atmosphere.
Described safety is special u-shaped glass sampling tube with filtering device 3, is inside provided with quartz particles interlayer.Characteristics of contaminated respiratory droplets
Moisture in gas is higher, can filter a part of vapor by quartz particles, after improving the organic pollutions such as paes
The continuous accuracy measuring；Meanwhile, when another effect of safety and filtering device 3 is to be prevented from absorbent suck-back, absorbent
Granule enters human body via oral cavity, thus working the mischief to health.
It is placed with the absorbent through pre-treatment in described absorber 4.
Claims (3)
1. a kind of method collecting paes in human body exhaled gas is it is characterised in that comprise the steps:
Step 1, with soxhlet extraction, pre-treatment is carried out to absorbent；
1-1. loads absorbent in handss seam gauze bag, then handss seam gauze bag is put in apparatus,Soxhlet'ses, under apparatus,Soxhlet'ses
Termination flask, pours detergent dichloromethane into apparatus,Soxhlet'ses, until the amount energy submergence gauze bag of detergent dichloromethane is
Only；The bath temperature of flask is controlled at 60 DEG C～65 DEG C, carries out washing for the first time, wash time is 46h～50h；
After the completion of 1-2. washs for the first time, it is cooled to room temperature, abandons the detergent dichloromethane in most flask, then to surname extraction
Detergent methanol is poured again into, till the amount energy submergence gauze bag of detergent methanol, now by the water-bath temperature of flask in device
Degree controls at 80 DEG C～83 DEG C, carries out second washing, and wash time is 46h～50h；
After the completion of step 2, twice washing, the absorbent in gauze bag is taken out, uses rotary evaporator revolving under negative atmospheric pressure
To being dried, the oil bath temperature of revolving controls at 44 DEG C～46 DEG C；Then absorbent is placed on closed glass containers for future use；
Step 3, by the collection and confinement of gases of characteristics of contaminated respiratory droplets in the absorber equipped with organic resin absorbent, absorbent can absorb people
Paes in the gas of body exhalation；
A kind of device of being used of method collecting paes in human body exhaled gas, including acquisition port, safety and filtering device and
Absorber；Acquisition port is patch skin-type full bag mouth dew nose formula plastic mask, and both sides are coupled with rubber band, are fixed on two ears, tested
Holding snuffing gas in journey, exhaled with mouth；Acquisition port is connected by rubber tube with the air inlet of filtering device with safety, safety with
The gas outlet of filtering device is connected by rubber tube with the air inlet of absorber；Absorber upper end is provided with gas outlet, with the external world
It is connected；
Described safety is special u-shaped glass sampling tube with filtering device, is inside provided with quartz particles interlayer；
It is placed with the absorbent through pre-treatment in described absorber.
2. as claimed in claim 1 a kind of method collecting paes in human body exhaled gas it is characterised in that described absorption
Agent is the organic resin of polyethylene kind.
3. as claimed in claim 1 a kind of method collecting paes in human body exhaled gas it is characterised in that described washing
It is pure that agent dichloromethane and methanol are analysis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410694843.6A CN104458348B (en) | 2014-11-27 | 2014-11-27 | Method and device for collecting PAEs in gas exhaled by human body |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410694843.6A CN104458348B (en) | 2014-11-27 | 2014-11-27 | Method and device for collecting PAEs in gas exhaled by human body |
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| Publication Number | Publication Date |
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| CN104458348A CN104458348A (en) | 2015-03-25 |
| CN104458348B true CN104458348B (en) | 2017-02-01 |
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| CN201410694843.6A Expired - Fee Related CN104458348B (en) | 2014-11-27 | 2014-11-27 | Method and device for collecting PAEs in gas exhaled by human body |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112841069B (en) * | 2021-02-26 | 2022-11-15 | 公安部南昌警犬基地 | Auxiliary device is distinguished to lung cancer based on people's expired gas |
| CN114487190B (en) * | 2022-01-25 | 2023-10-03 | 南京信息工程大学 | Medical human body exhale VOC gas detection device based on light PID sensor |
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|---|---|---|---|---|
| US4079621A (en) * | 1976-05-28 | 1978-03-21 | E. I. Du Pont De Nemours And Company | Apparatus for determining the dust index of a particulate solid |
| US4092845A (en) * | 1976-05-28 | 1978-06-06 | Comitato Nazionale per l'Energia Nucleare -- Cnen | Lung simulating aerosol sampler |
| US5266496A (en) * | 1992-04-10 | 1993-11-30 | Dacruz Amelia L | Headspace analysis |
| CN2310992Y (en) * | 1996-04-17 | 1999-03-17 | 李成刚 | Multifunctional breath synchronous sampler |
| DE20211433U1 (en) * | 2002-07-12 | 2002-10-17 | Fuchs Juergen | Passive collector for volatile air pollutants |
| CN203178240U (en) * | 2013-04-15 | 2013-09-04 | 江苏省环境监测中心 | Anti-jamming device for determining phthalate acid esters |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7101341B2 (en) * | 2003-04-15 | 2006-09-05 | Ross Tsukashima | Respiratory monitoring, diagnostic and therapeutic system |
-
2014
- 2014-11-27 CN CN201410694843.6A patent/CN104458348B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4079621A (en) * | 1976-05-28 | 1978-03-21 | E. I. Du Pont De Nemours And Company | Apparatus for determining the dust index of a particulate solid |
| US4092845A (en) * | 1976-05-28 | 1978-06-06 | Comitato Nazionale per l'Energia Nucleare -- Cnen | Lung simulating aerosol sampler |
| US5266496A (en) * | 1992-04-10 | 1993-11-30 | Dacruz Amelia L | Headspace analysis |
| CN2310992Y (en) * | 1996-04-17 | 1999-03-17 | 李成刚 | Multifunctional breath synchronous sampler |
| DE20211433U1 (en) * | 2002-07-12 | 2002-10-17 | Fuchs Juergen | Passive collector for volatile air pollutants |
| CN203178240U (en) * | 2013-04-15 | 2013-09-04 | 江苏省环境监测中心 | Anti-jamming device for determining phthalate acid esters |
Non-Patent Citations (3)
| Title |
|---|
| Concentration and risk assessment of phthalates present in indoor air from newly decorated apartments;X. Q. Pei et al.;《Atmospheric Environment》;20131231(第68期);第18页第二栏第2、3段 * |
| 住宅室内降尘中邻苯二甲酸酯污染特征及暴露评价;王夫美 等;《中国环境科学》;20121231;第32卷(第5期);第780-786段 * |
| 夏季学生宿舍室内降尘中PAEs污染特征和暴露评价;邹德坤 等;《中国环境科学》;20131231;第33卷(第11期);第2075-2080段 * |
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| CN104458348A (en) | 2015-03-25 |
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Granted publication date: 20170201 Termination date: 20191127 |