CN104458348A - Method and device for collecting PAEs in gas exhaled by human body - Google Patents
Method and device for collecting PAEs in gas exhaled by human body Download PDFInfo
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- CN104458348A CN104458348A CN201410694843.6A CN201410694843A CN104458348A CN 104458348 A CN104458348 A CN 104458348A CN 201410694843 A CN201410694843 A CN 201410694843A CN 104458348 A CN104458348 A CN 104458348A
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- human body
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Abstract
The invention discloses a method and a device for collecting PAEs in gas exhaled by a human body. The method comprises the following steps: step 1, carrying out pretreatment on an absorbent by using a Soxhlet extraction method; step 2, after the completion of the pretreatment, taking out the absorbent in a gauze bag and carrying out rotary evaporation under the negative atmospheric pressure till the absorbent is dry, wherein the oil bath temperature in the rotary evaporation is controlled within 44-46 DEG C; then putting the absorbent in an airtight glass container for later use; step 3, collecting the gas exhaled by the human body into an absorber filled with the absorbent, wherein the absorbent can absorb the PAEs in the gas exhaled by the human body and other organic pollutants. According to the method and the device, the collection of specific volatile pollutants, semi-volatile organic pollutants and the like by the human body through a respiratory pathway can be simply and feasibly realized, so that a basis is provided for further researching the absorption efficiency of a human body respiratory tract, evaluating the effect of specific pollutants in indoor air on human body health and the like.
Description
Technical field
The chemical environment that the invention belongs to environmental chemistry is polluted and health field, particularly relates to a kind of method and the operative installations thereof of collecting PAEs in human body breath.
Background technology
For a long time, the environmental contaminants of low concentration are a difficult problem for ecotoxicology research field on the impact of health always, and its Research Challenges is be difficult to determine the absorption efficiency of human body to pollutant first.At present, similar invention or document relate to characteristics of contaminated respiratory droplets gas area research, mainly concentrate in detection method, as: the simultaneously integrated analysis device (application publication number: CN103168233A) of EBCs and VOCs in human body breath, and for example: a kind of method (application publication number: CN102507720A) of NO content in human body breath.So far also there is no an invention, relate to characteristics of contaminated respiratory droplets gas collector specially.Market there is no the instrument about collection human body breath that can buy; And for the research of the above-mentioned type, in its experimentation, if desired collect human body breath, either complicated operation, process take time and effort, or instrument price is expensive.
At present, the home ventilator that on market, degree of recognition is higher has the two level respirator of Rui Simai S9 VPAP ST, the two level respirators of the big health BiPAP S/T in Philip etc., its price is all between 10,000 yuan ~ 20,000 yuans, what hospital intensive care unit etc. used has wound lung ventilator, and price is then higher.The present invention is different from above-mentioned medical auxiliary equipment human body respiration device, is mainly used in pollutant in air to the research of the aspects such as Health Impact.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of method and the operative installations thereof of collecting PAEs in human body breath are provided.
The technical solution adopted for the present invention to solve the technical problems is as follows:
Collect a method of PAEs in human body breath, comprise the steps:
Step 1, with soxhlet extraction, pre-treatment is carried out to absorbing agent;
Absorbing agent loads in hand seam gauze bag by 1-1., then hand is stitched gauze bag puts into apparatus,Soxhlet's, and termination flask under apparatus,Soxhlet's, pours washing agent methylene chloride into apparatus,Soxhlet's, till the amount energy submergence gauze bag of washing agent methylene chloride; Controlled by the bath temperature of flask at 60 DEG C ~ 65 DEG C, carry out first time washing, wash time is 46h ~ 50h;
After 1-2. has washed for the first time, to be cooled to normal temperature, abandon the washing agent methylene chloride in most flask, then in apparatus,Soxhlet's, washing agent methyl alcohol is again poured into, till the amount energy submergence gauze bag of washing agent methyl alcohol, now controlled by the bath temperature of flask at 80 DEG C ~ 83 DEG C, carry out second time washing, wash time is 46h ~ 50h;
After step 2, twice washing complete, taken out by the absorbing agent in gauze bag, steam to dry at negative atmospheric pressure backspin, the oil bath temperature revolving steaming controls at 44 DEG C ~ 46 DEG C; Then absorbing agent is placed on closed glass containers for future use;
Step 3, by the gas collection of characteristics of contaminated respiratory droplets in the absorber that absorbing agent is housed, absorbing agent can absorb the organic contaminant in the gas of characteristics of contaminated respiratory droplets.
Described absorbing agent is the organic resin of polyethylene kind, as: the absorbing agent for PAEs is XAD-2 resin, and the absorbing agent for VOC is Tenax-TA resin.
It is pure that described washing agent methylene chloride and methyl alcohol are analysis.
The device that the method for collecting PAEs in human body breath uses, comprises acquisition port, safety and filtering device and absorber; Acquisition port is for pasting skin-type full bag mouth dew nose formula plastic mask, and both sides rubber band connects, and is fixed on two ears, maintenance snuffing gas in process of the test, exhales with mouth; Acquisition port is connected by proofed sleeve with the air intake opening of filtering device with safety, and safety is connected by proofed sleeve with the air intake opening of absorber with the gas outlet of filtering device; Absorber upper end is provided with gas outlet, is connected with the external world.
Described safety and filtering device are special U-shaped sampling glass tube, are inside provided with quartz particles interlayer.
The absorbing agent through pre-treatment is placed with in described absorber.
Beneficial effect of the present invention is as follows:
The present invention is different from the medical auxiliary equipment such as commercially available respirator on market, can realize to simple possible human body by the collection of respiratory pathways to the pollutant in special air, realize the sampling respectively to human body suck pollution substrate concentration and exhalation pollutant levels, thus for research specific pollutants respiratory tract absorption efficiency, the pollutant assessed in room air make place mat to researchs such as the impacts of health further.
Apparatus of the present invention can realize the sampling respectively to human body suck pollution substrate concentration and exhalation pollutant levels, for subsequent measurements and correlative study realize providing feasible approach.
Accompanying drawing explanation
Fig. 1 is apparatus of the present invention schematic diagram.
In figure: acquisition port 1, air intake opening 2, safety and filtering device 3, absorber 4, gas outlet 5.
Embodiment
Collect a method of PAEs in human body breath, specifically comprise the steps:
Step 1, with soxhlet extraction, pre-treatment is carried out to absorbing agent.
Absorbing agent loads in hand seam gauze bag by 1-1., then hand is stitched gauze bag puts into apparatus,Soxhlet's, and termination flask under apparatus,Soxhlet's, pours washing agent methylene chloride into apparatus,Soxhlet's, till the amount submergence gauze bag of washing agent methylene chloride; Controlled by the bath temperature of flask at 60 ~ 65 DEG C, carry out first time washing, wash time is 46h ~ 50h;
After 1-2. has washed for the first time, to be cooled to normal temperature, abandon the washing agent methylene chloride in most flask, then in apparatus,Soxhlet's, washing agent methyl alcohol is again poured into, till the amount submergence gauze bag of washing agent methyl alcohol, now control at 80 ~ 83 DEG C by the bath temperature of flask, carry out second time washing, wash time is 46h ~ 50h;
Described absorbing agent is the organic resin of polyethylene kind, as: the absorbing agent for PAEs is XAD-2 resin, and the absorbing agent for VOC is Tenax-TA resin;
It is pure that described washing agent methylene chloride and methyl alcohol are analysis.
After step 2, twice washing complete, taken out by absorbing agent in gauze bag, revolve with rotary evaporator RE-52AA under negative atmospheric pressure and steam to dry, the oil bath temperature revolving steaming controls at 44 DEG C ~ 46 DEG C; Then absorbing agent is placed on closed glass containers for future use.
Described negative atmospheric pressure, without strict demand, uses common SHB-
the multiplex vacuum pump of circulating water type etc. vacuumizes.
Step 3, by the gas collection of characteristics of contaminated respiratory droplets in the absorber of organic resin absorbing agent that polyethylene kind is housed, absorbing agent can absorb the organic contaminants such as the PAEs in the gas of characteristics of contaminated respiratory droplets.
The device that the method for collecting PAEs in human body breath uses, comprises acquisition port 1, safety and filtering device 3 and absorber 4; Acquisition port 1 is for pasting skin-type full bag mouth dew nose formula plastic mask, and both sides rubber band connects, and is fixed on two ears, maintenance snuffing gas in process of the test, exhales with mouth; Acquisition port 1 is connected by proofed sleeve with the air intake opening 2 of filtering device 3 with safety, and safety is connected by proofed sleeve with the air intake opening of absorber 4 with the gas outlet of filtering device 3; Absorber 4 upper end is provided with gas outlet 5, and gas outlet 5 is connected with ambient atmosphere.
Described safety and filtering device 3 are special U-shaped sampling glass tube, are inside provided with quartz particles interlayer.Moisture in characteristics of contaminated respiratory droplets gas is higher, can filter a part of water vapor by quartz particles, improves the accuracy of the organic contaminant subsequent measurements such as PAEs; Meanwhile, it is that when can prevent absorbing agent suck-back, absorbent granules enters human body via oral cavity, thus works the mischief to health that another of safety and filtering device 3 acts on.
The absorbing agent through pre-treatment is placed with in described absorber 4.
Claims (6)
1. collect a method of PAEs in human body breath, it is characterized in that comprising the steps:
Step 1, with soxhlet extraction, pre-treatment is carried out to absorbing agent;
Absorbing agent loads in hand seam gauze bag by 1-1., then hand is stitched gauze bag puts into apparatus,Soxhlet's, and termination flask under apparatus,Soxhlet's, pours washing agent methylene chloride into apparatus,Soxhlet's, till the amount energy submergence gauze bag of washing agent methylene chloride; Controlled by the bath temperature of flask at 60 DEG C ~ 65 DEG C, carry out first time washing, wash time is 46h ~ 50h;
After 1-2. has washed for the first time, be cooled to normal temperature, abandon the washing agent methylene chloride in most flask, then in apparatus,Soxhlet's, washing agent methyl alcohol is again poured into, till the amount energy submergence gauze bag of washing agent methyl alcohol, now controlled by the bath temperature of flask at 80 DEG C ~ 83 DEG C, carry out second time washing, wash time is 46h ~ 50h;
After step 2, twice washing complete, taken out by the absorbing agent in gauze bag, revolve with rotary evaporator under negative atmospheric pressure and steam to dry, the oil bath temperature revolving steaming controls at 44 DEG C ~ 46 DEG C; Then absorbing agent is placed on closed glass containers for future use;
Step 3, by the gas collection of characteristics of contaminated respiratory droplets in the absorber that organic resin absorbing agent is housed, absorbing agent can absorb the PAEs in the gas of characteristics of contaminated respiratory droplets.
2. a kind of method of collecting PAEs in human body breath as claimed in claim 1, is characterized in that, it is characterized in that described absorbing agent is the organic resin of polyethylene kind.
3. a kind of method of collecting PAEs in human body breath as claimed in claim 1, is characterized in that, it is characterized in that described washing agent methylene chloride and methyl alcohol are analysis pure.
4. the device that the method for collecting PAEs in human body breath uses, is characterized in that comprising acquisition port, safety and filtering device and absorber; Acquisition port is for pasting skin-type full bag mouth dew nose formula plastic mask, and both sides rubber band connects, and is fixed on two ears, maintenance snuffing gas in process of the test, exhales with mouth; Acquisition port is connected by proofed sleeve with the air intake opening of filtering device with safety, and safety is connected by proofed sleeve with the air intake opening of absorber with the gas outlet of filtering device; Absorber upper end is provided with gas outlet, is connected with the external world.
5. the device that uses of a kind of method of collecting PAEs in human body breath as claimed in claim 4, is characterized in that described safety and filtering device are special U-shaped sampling glass tube, is inside provided with quartz particles interlayer.
6. the device that uses of a kind of method of collecting PAEs in human body breath as claimed in claim 4, is characterized in that the absorbing agent be placed with in described absorber through pre-treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410694843.6A CN104458348B (en) | 2014-11-27 | 2014-11-27 | Method and device for collecting PAEs in gas exhaled by human body |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410694843.6A CN104458348B (en) | 2014-11-27 | 2014-11-27 | Method and device for collecting PAEs in gas exhaled by human body |
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| CN104458348A true CN104458348A (en) | 2015-03-25 |
| CN104458348B CN104458348B (en) | 2017-02-01 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112841069A (en) * | 2021-02-26 | 2021-05-28 | 公安部南昌警犬基地 | Training method and auxiliary device for identifying dogs for lung cancer based on exhaled air of people |
| CN114487190A (en) * | 2022-01-25 | 2022-05-13 | 南京信息工程大学 | Medical human body exhaled VOC gas detection device based on optical PID sensor |
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| US4092845A (en) * | 1976-05-28 | 1978-06-06 | Comitato Nazionale per l'Energia Nucleare -- Cnen | Lung simulating aerosol sampler |
| US5266496A (en) * | 1992-04-10 | 1993-11-30 | Dacruz Amelia L | Headspace analysis |
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| X. Q. PEI ET AL.: "Concentration and risk assessment of phthalates present in indoor air from newly decorated apartments", 《ATMOSPHERIC ENVIRONMENT》 * |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112841069A (en) * | 2021-02-26 | 2021-05-28 | 公安部南昌警犬基地 | Training method and auxiliary device for identifying dogs for lung cancer based on exhaled air of people |
| CN114487190A (en) * | 2022-01-25 | 2022-05-13 | 南京信息工程大学 | Medical human body exhaled VOC gas detection device based on optical PID sensor |
| CN114487190B (en) * | 2022-01-25 | 2023-10-03 | 南京信息工程大学 | Medical human body exhale VOC gas detection device based on light PID sensor |
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