CN210794115U

CN210794115U - Kit for DNA seamless cloning

Info

Publication number: CN210794115U
Application number: CN201921662365.5U
Authority: CN
Inventors: 常静瑶
Original assignee: Tianjin Anbisen Bioscience Co ltd
Current assignee: Tianjin Anbisen Bioscience Co ltd
Priority date: 2019-09-30
Filing date: 2019-09-30
Publication date: 2020-06-19
Anticipated expiration: 2029-09-30

Abstract

The utility model discloses a kit for seamless cloning of DNA, the power distribution box comprises a box body, box roof central point puts and has seted up first mounting groove, diapire fixedly connected with installation piece in the first mounting groove, a plurality of standing grooves have been seted up to installation piece roof, symmetrical fixedly connected with dead lever around box roof one side, the box roof is kept away from dead lever one side intermediate position and is seted up flutedly. The utility model discloses a have a plurality of standing grooves to place the reagent that is used for the seamless cloning of DNA, the opening part can be covered and close, protects the inside reagent that is used for the seamless cloning of DNA of depositing, and the lid closes the mechanism and is automatic elevating system, and degree of automation is higher, and it is comparatively convenient high-efficient when the access is used for the reagent of the seamless cloning of DNA.

Description

Kit for DNA seamless cloning

Technical Field

The utility model relates to a kit technical field especially relates to a kit for seamless cloning of DNA.

Background

The Seamless Cloning (Seamless Cloning/In-Fusion Cloning) is different from the traditional PCR product Cloning, the only difference is that the tail end of a vector and the tail end of a primer have 15-20 homologous bases, 15-20 bases which are homologous with a vector sequence are respectively carried at two ends of the obtained PCR product, complementary pairing and cyclization are realized by virtue of the acting force between the bases, the PCR product can be directly used for transforming host bacteria without enzyme connection, and a linear plasmid (circular) entering the host bacteria repairs gaps by virtue of a self enzyme system.

At present, more reagents for DNA seamless cloning exist, the reagents are stored and used often in a centralized management manner, the existing kit has poor protection effect and inconvenient access, in addition, the automation degree is low, and the efficiency is poor when the reagents for DNA seamless cloning are actually accessed.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects in the prior art and providing a kit for seamless cloning of DNA.

In order to achieve the above purpose, the utility model adopts the following technical scheme: the utility model provides a kit for seamless cloning of DNA, includes the box, box roof central point puts and has seted up first mounting groove, diapire fixedly connected with installation piece in the first mounting groove, a plurality of standing grooves have been seted up to installation piece roof, symmetrical fixedly connected with dead lever around box roof one side, the box roof is kept away from dead lever one side intermediate position and is seted up flutedly, the second mounting groove has been seted up to the diapire in the recess, diapire central point puts fixed mounting and has the motor in the second mounting groove, motor output shaft fixedly connected with screw rod, two dead lever sliding connection has same mounting panel, mounting panel diapire central point puts the fixedly connected with stopper.

Preferably, the mounting block is fixedly connected with the inner side wall of a first mounting groove, the mounting block is made of hard rubber, and the placing grooves are arranged in equidistant rectangular arrays.

Preferably, sliding grooves are symmetrically formed in the mounting plate between the upper side wall and the lower side wall of one side, close to the fixing rod, of the mounting plate in a front-back mode, and the fixing rod on the side is connected in the sliding grooves in a sliding mode.

Preferably, a thread groove is formed between the upper side wall and the lower side wall of the middle position of one side, away from the fixing rod, of the mounting plate, and a screw rod on the side is in threaded connection with the thread groove.

Preferably, the screw is slidably connected in the groove.

Preferably, the limiting block is located above the first mounting groove, and a plurality of sealing rings are fixedly sleeved on the peripheral side wall of the limiting block at equal intervals.

The utility model discloses following beneficial effect has:

compared with the prior art, the utility model, adopt to have a plurality of standing grooves to place the reagent that is used for the seamless cloning of DNA, the opening part can cover and close, and the inside reagent that is used for the seamless cloning of DNA of depositing of protection, the lid closes the mechanism and is automatic elevating system, and degree of automation is higher, and it is comparatively convenient high-efficient when using the reagent that is used for the seamless cloning of DNA to access.

Drawings

Fig. 1 is a front view of the present invention.

Fig. 2 is a schematic view of the structure of the present invention.

Fig. 3 is a top view of the present invention.

Illustration of the drawings:

1. a box body; 2. a first mounting groove; 3. fixing the rod; 4. mounting a plate; 5. a limiting block; 6. a groove; 7. a second mounting groove; 8. a motor; 9. a screw; 10. a chute; 11. a thread groove; 12. a seal ring; 13. mounting blocks; 14. and (6) placing the groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-3, the present invention provides an embodiment: the utility model provides a kit for seamless cloning of DNA, the power distribution box comprises a

box body

1, 1 roof central point of box puts and has seted up first mounting groove 2, wall fixedly connected with installation piece 13 in 2 first mounting grooves, a plurality of standing grooves 14 have been seted up to installation piece 13 roof, place and be equipped with the test tube that is used for the seamless cloned reagent of DNA, symmetrical fixedly connected with dead lever 3 around 1 roof one side of box, 3 one side intermediate positions of dead lever are kept away from to 1 roof of box are seted up flutedly 6, second mounting groove 7 has been seted up to the wall in the recess 6, wall central point fixedly connected with motor 8 in 7 second mounting grooves, motor 8 output shaft fixedly connected with screw rod 9, driving screw rod 9 rotates, 3 sliding connection of two dead levers have same mounting panel 4, mounting panel 4 diapire central point fixedly connected with stopper 5, play.

2 inside walls of the first mounting groove of 13 lateral walls fixedly connected with of installation piece, installation piece 13 is the stereoplasm rubber material, play buffering cushioning effect, a plurality of standing grooves 14 are equidistant rectangular array and arrange, it is convenient to accomodate the test tube that uses the reagent that is used for the seamless cloning of DNA, mounting panel 4 has seted up spout 10 near the symmetry about 3 one side of dead lever between the wall of both sides, sliding connection has the dead lever 3 of this side in the spout 10, mounting panel 4 keeps away from and has seted up thread groove 11 about 3 one side intermediate position of dead lever between the wall of both sides, 11 female connections in thread groove have the screw rod 9 of this side, 9 sliding connection of screw rod is in recess 6, stopper 5 is located 2 tops in first mounting groove, stopper 5 fixed cover all around of lateral wall equidista.

The working principle is as follows: when in use, the test tube of the reagent for DNA seamless cloning is stored in a plurality of placing grooves 14, the placing grooves 14 are arranged on a mounting block 13, the mounting block 13 is made of hard rubber and plays a role of protecting the test tube of the reagent for DNA seamless cloning, at this time, a motor 8 is switched on, a driving screw rod 9 is driven to rotate, because the screw 9 is in threaded connection with the thread groove 11, the thread groove 11 is arranged on one side of the mounting plate 4, the other side of the mounting plate 4 is provided with the sliding groove 10, the sliding groove 10 is in sliding connection with the fixed rod 3, when the screw 9 rotates, the mounting plate 4 can move along the fixed rod 3, the moving direction of the mounting plate 4 can be controlled by the rotating direction of the screw 9, namely, the mounting plate 4 is lifted, when the mounting plate is lowered to the lowest end, the limiting block 5 is embedded into the first mounting groove 2 to seal the opening of the first mounting groove 2, the test tubes of the reagent for seamless cloning of DNA placed in the placement tank 14 are protected.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A kit for seamless cloning of DNA, comprising a box body (1), characterized in that: box (1) roof central point puts and has seted up first mounting groove (2), wall fixedly connected with installation piece (13) in first mounting groove (2), a plurality of standing grooves (14) have been seted up to installation piece (13) roof, symmetrical fixedly connected with dead lever (3) around box (1) roof one side, dead lever (3) one side intermediate position is kept away from to box (1) roof and is seted up fluted (6), second mounting groove (7) have been seted up to the wall in recess (6), wall central point puts fixed mounting has motor (8) in second mounting groove (7), motor (8) output shaft fixedly connected with screw rod (9), two dead lever (3) sliding connection has same mounting panel (4), mounting panel (4) diapire central point puts fixedly connected with stopper (5).

2. The kit for seamless cloning of DNA according to claim 1, wherein: the mounting block (13) is characterized in that the side wall of the mounting block (13) is fixedly connected with the inner side wall of the first mounting groove (2), the mounting block (13) is made of hard rubber, and the placing grooves (14) are arranged in equidistant rectangular arrays.

3. The kit for seamless cloning of DNA according to claim 1, wherein: the mounting plate (4) is close to and has seted up spout (10) around the symmetry between the wall of both sides about dead lever (3) one side, sliding connection has dead lever (3) of this side in spout (10).

4. The kit for seamless cloning of DNA according to claim 1, wherein: thread groove (11) have been seted up between the wall about mounting panel (4) keep away from dead lever (3) one side intermediate position, thread groove (11) female connection has screw rod (9) of this side.

5. The kit for seamless cloning of DNA according to claim 1, wherein: the screw rod (9) is connected in the groove (6) in a sliding mode.

6. The kit for seamless cloning of DNA according to claim 1, wherein: stopper (5) are located first mounting groove (2) top, stopper (5) lateral wall equidistance all around is fixed cup jointed a plurality of sealing washers (12).